GJB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJB2
Entrez Gene ID	2706
Full Name	gap junction protein beta 2
Synonyms	CX26,DFNA3,DFNA3A,DFNB1,DFNB1A,HID,KID,NSRD1,PPK
General protein information	Preferred Names gap junction protein beta 2 Names gap junction beta-2 protein connexin 26 gap junction protein, beta 2, 26kDa mutant gap junction protein beta 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.11
Summary	This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008].
Disorder MIM:	121011
Disorder Html:	Deafness, autosomal recessive 1A, 220290 (3); Deafness, autosomal dominant 3A, 601544 (3); Vohwinkel syndrome, 124500 (3); Keratoderma, palmoplantar, with deafness, 148350 (3); Keratitis-ichthyosis-deafness syndrome, 148210 (3); Hystrix-like ichthyosis with deafness, 602540 (3); Bart-Pumphrey syndrome, 149200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011535049.1	XP_011533351.1	gap junction beta-2 protein isoform X1
XM_011535049.2	XP_011533351.1	gap junction beta-2 protein isoform X1
NM_004004.5	NP_003995.2	gap junction beta-2 protein
NM_004004.6	NP_003995.2	gap junction beta-2 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GJB2	XP_522618.1
Rattus norvegicus (Norway rat)	Gjb2	NP_001004099.1
Gallus gallus (chicken)	GJB2	NP_001257745.1
Homo sapiens (human)	GJB2	NP_003995.2
Canis lupus familiaris (dog)	GJB2	NP_001184077.1
Mus musculus (house mouse)	Gjb2	NP_032151.1
	GJB2	NP_001038200.1
Bos taurus (cattle)	GJB2	NP_001077106.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Testing of Non-familial Deaf Patients for CIB2 and GJB2 Mutations: Phenotype and Genetic Counselling.
Shaikh H, Waryah AM, Narsani AK, Iqbal M, Shahzad M, Waryah YM, Shaikh N, Mahmood A
Biochemical genetics55(5-6)410-420(2017 Dec)

GJB2 mutations: Genotypic and phenotypic correlation in a cohort of 690 hearing-impaired patients, toward a new mutation?
Lecl?re JC, Le Gac MS, Le Mar?chal C, Ferec C, Marianowski R
International journal of pediatric otorhinolaryngology10280-85(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WFS1 and GJB2 mutations were identified in eight of 74 cases of Low-Frequency Sensorineural Hearing Loss. Four cases had heterozygous WFS1 mutations; one had a heterozygous WFS1 mutation and a heterozygous GJB2 mutation; and three cases had biallelic GJB2 mutations. Three cases with WFS1 mutations were sporadic; two of them were confirmed to be caused by a de novo mutation based on the genetic analysis of their parents.
Title: WFS1 and GJB2 mutations in patients with bilateral low-frequency sensorineural hearing loss.

GJB2-related deafness leads to significantly better cochlear implantation outcomes when compared with acquired deafness caused by environmental etiologies. However, GJB2 mutation is not associated with a significantly better prognosis when compared with those whose deafness results from either nonsyndromic hearing loss of unknown origin or other types of genetic mutations in the absence of other neurologic deficits.
Title: Predictive Value of GJB2 Mutation Status for Hearing Outcomes of Pediatric Cochlear Implantation.

results demonstrate that 19.2% patients with nonsyndromic deafness were caused by mutations in three common deafness genes (GJB2, SLC26A4 and 12S rRNA) in our northern China patient group
Title: Mutation analysis of common GJB2, SCL26A4 and 12S rRNA genes among 380 deafness patients in northern China.

study suggests that the GJB2 235delC polymorphism, but not the 30-35delG variant, contributes to congenital deafness susceptibility in the Chinese population examined
Title: Effect of GJB2 235delC and 30-35delG genetic polymorphisms on risk of congenital deafness in a Chinese population.

Reduced expression of Cx26 and Cx43 is implicated in the pathophysiology of colonic dysmotility in the aganglionic bowel as well as, in the case of Cx26, the ganglionic bowel in Hirschsprung's disease.
Title: Expression of connexin 26 and connexin 43 is reduced in Hirschsprung's disease.

The following GJB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27143	NM_004004.6 Latest version!	Homo sapiens gap junction protein beta 2 (GJB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27143	XM_011535049.2 Latest version!	Homo sapiens gap junction protein beta 2 (GJB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu27143	XM_011535049.1	Homo sapiens gap junction protein, beta 2, 26kDa (GJB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu27143	NM_004004.5	Homo sapiens gap junction protein beta 2 (GJB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJB2 ( NM_004004.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003995.2 Homo sapiens gap junction protein beta 2 (GJB2), mRNA.

Price & Availability↑

CloneID	OHu27143
Clone ID Related Accession (Same CDS sequence)	XM_011535049.1 , XM_011535049.2 , NM_004004.5 , NM_004004.6
Accession Version	NM_004004.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	gap junction beta-2 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA287424.1, BC017048.1, BC071703.1, AA962716.1 and BI491091.1. This sequence is a reference standard in the RefSeqGene project. On Aug 2, 2008 this sequence version replaced NM_004004.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279855.3065.1, SRR1660805.117664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003995.2
CDS	216..896
Translation	MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Target ORF information:

RefSeq Version	NM_004004.5
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein beta 2 (GJB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004004.5

ORF Insert Sequence:

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJB2 ( NM_004004.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_003995.2 Homo sapiens gap junction protein beta 2 (GJB2), mRNA.

Price & Availability↑

CloneID	OHu27143
Clone ID Related Accession (Same CDS sequence)	XM_011535049.1 , XM_011535049.2 , NM_004004.5 , NM_004004.6
Accession Version	NM_004004.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	gap junction beta-2 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA287424.1, BC017048.1, BC071703.1 and AA962716.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004004.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279855.3065.1, SRR1660805.117664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000382848.5/ ENSP00000372299.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003995.2
CDS	179..859
Translation	MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Target ORF information:

RefSeq Version	NM_004004.6
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein beta 2 (GJB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004004.6

ORF Insert Sequence:

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJB2 ( XM_011535049.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533351.1 Homo sapiens gap junction protein, beta 2, 26kDa (GJB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27143
Clone ID Related Accession (Same CDS sequence)	XM_011535049.1 , XM_011535049.2 , NM_004004.5 , NM_004004.6
Accession Version	XM_011535049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	gap junction beta-2 protein isoform X1
Comment

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533351.1
Translation	MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Target ORF information:

RefSeq Version	XM_011535049.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens gap junction protein, beta 2, 26kDa (GJB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535049.1

ORF Insert Sequence:

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJB2 ( XM_011535049.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533351.1 Homo sapiens gap junction protein beta 2 (GJB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu27143
Clone ID Related Accession (Same CDS sequence)	XM_011535049.1 , XM_011535049.2 , NM_004004.5 , NM_004004.6
Accession Version	XM_011535049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gap junction beta-2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533351.1
CDS	207..887
Translation	MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Target ORF information:

RefSeq Version	XM_011535049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein beta 2 (GJB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535049.2

ORF Insert Sequence:

ATGGATTGGG GCACGCTGCA GACGATCCTG GGGGGTGTGA ACAAACACTC CACCAGCATT 
GGAAAGATCT GGCTCACCGT CCTCTTCATT TTTCGCATTA TGATCCTCGT TGTGGCTGCA 
AAGGAGGTGT GGGGAGATGA GCAGGCCGAC TTTGTCTGCA ACACCCTGCA GCCAGGCTGC 
AAGAACGTGT GCTACGATCA CTACTTCCCC ATCTCCCACA TCCGGCTATG GGCCCTGCAG 
CTGATCTTCG TGTCCACGCC AGCGCTCCTA GTGGCCATGC ACGTGGCCTA CCGGAGACAT 
GAGAAGAAGA GGAAGTTCAT CAAGGGGGAG ATAAAGAGTG AATTTAAGGA CATCGAGGAG 
ATCAAAACCC AGAAGGTCCG CATCGAAGGC TCCCTGTGGT GGACCTACAC AAGCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCCGCCTTC ATGTACGTCT TCTATGTCAT GTACGACGGC 
TTCTCCATGC AGCGGCTGGT GAAGTGCAAC GCCTGGCCTT GTCCCAACAC TGTGGACTGC 
TTTGTGTCCC GGCCCACGGA GAAGACTGTC TTCACAGTGT TCATGATTGC AGTGTCTGGA 
ATTTGCATCC TGCTGAATGT CACTGAATTG TGTTATTTGC TAATTAGATA TTGTTCTGGG 
AAGTCAAAAA AGCCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Testing of Non-familial Deaf Patients for CIB2 and GJB2 Mutations: Phenotype and Genetic Counselling.
Biochemical genetics55(5-6)410-420(2017 Dec)
Shaikh H,Waryah AM,Narsani AK,Iqbal M,Shahzad M,Waryah YM,Shaikh N,Mahmood A

GJB2 mutations: Genotypic and phenotypic correlation in a cohort of 690 hearing-impaired patients, toward a new mutation?
International journal of pediatric otorhinolaryngology10280-85(2017 Nov)
Lecl?re JC,Le Gac MS,Le Mar?chal C,Ferec C,Marianowski R

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GJB2 cDNA ORF clone, Homo sapiens(human)

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