GHRH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GHRH
Entrez Gene ID	2691
Full Name	growth hormone releasing hormone
Synonyms	GHRF,GRF,INN
General protein information	Preferred Names growth hormone releasing hormone Names somatoliberin growth hormone releasing factor sermorelin somatocrinin somatorelin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.23
Summary	This gene encodes a member of the glucagon family of proteins. The encoded preproprotein is produced in the hypothalamus and cleaved to generate the mature factor, known as somatoliberin, which acts to stimulate growth hormone release from the pituitary gland. Variant receptors for somatoliberin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016].
Disorder MIM:	139190
Disorder Html:	?Isolated growth hormone deficiency due to defect in GHRF (1); Gigantism due to GHRF hypersecretion (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184731.2	NP_001171660.1	somatoliberin isoform 2 preproprotein
XM_011528788.2	XP_011527090.1	somatoliberin isoform X2
XM_011528784.2	XP_011527086.1	somatoliberin isoform X1
NM_021081.5	NP_066567.1	somatoliberin isoform 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GHRH	NP_847895.1
Homo sapiens (human)	GHRH	NP_001171660.1
Mus musculus (house mouse)	Ghrh	NP_034415.1
Pan troglodytes (chimpanzee)	GHRH	XP_525318.2
	GHRH	XP_001093107.2
Canis lupus familiaris (dog)	GHRH	XP_542987.2
Rattus norvegicus (Norway rat)	Ghrh	NP_113765.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression-cloning and sequence of a cDNA encoding human growth hormone-releasing factor.
Mayo KE, Vale W, Rivier J, Rosenfeld MG, Evans RM
Nature306(5938)86-8(1983 Nov 3-9)

Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly.
Guillemin R, Brazeau P, B?hlen P, Esch F, Ling N, Wehrenberg WB
Science (New York, N.Y.)218(4572)585-7(1982 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report a high rate of GHRH-R and GHRH expression in apocrine breast carcinomas.
Title: The expression of GHRH and its receptors in breast carcinomas with apocrine differentiation-further evidence of the presence of a GHRH pathway in these tumors.

GHRH analogs of the Miami series powerfully suppress tumor growth, but have only a weak endocrine GH inhibitory activity. The suppression of tumor growth could be induced in part by the downregulation of
Title: Synthesis and structure-activity studies on novel analogs of human growth hormone releasing hormone (GHRH) with enhanced inhibitory activities on tumor growth.

GHRH stimulates a ligand-independent activation of EGFR involving at least cAMP/PKA and Src family signaling pathways
Title: Growth hormone-releasing hormone induced transactivation of epidermal growth factor receptor in human triple-negative breast cancer cells.

GHRH excess and blockade in X-LAG syndrome
Title: GHRH excess and blockade in X-LAG syndrome.

GHRH and GHRH-R loops are involved in placental choriocarcinoma cell line viability and apoptosis through Akt and eIF2a pathways.
Title: Impact of Axis of GHRH and GHRH Receptor on Cell Viability and Apoptosis of the Placental Choriocarcinoma Cell Line.

The following GHRH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GHRH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26804	NM_001184731.2 Latest version!	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu26946	NM_021081.5 Latest version!	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu26804	XM_011528788.2 Latest version!	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu26946	XM_011528784.2 Latest version!	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GHRH ( NM_001184731.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171660.1 Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26804
Clone ID Related Accession (Same CDS sequence)	NM_001184731.2 , XM_011528788.2
Accession Version	NM_001184731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	somatoliberin isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX102214.1, BC098161.1 and AW134884.1. On Jan 14, 2016 this sequence version replaced NM_001184731.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGGA ACTCCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171660.1
CDS	20..343
Translation	MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARLGRQVDSMWAEQKQMELESILVALLQKHRNSQG

Target ORF information:

RefSeq Version	NM_001184731.2
Organism	Homo sapiens(human)
Definition	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184731.2

ORF Insert Sequence:

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGGA ACTCCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRH ( NM_021081.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_066567.1 Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26946
Clone ID Related Accession (Same CDS sequence)	XM_011528784.2 , NM_021081.5
Accession Version	NM_021081.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	somatoliberin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX102214.1, BC098109.1 and AW134884.1. On Jan 14, 2016 this sequence version replaced NM_021081.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE348700.1, AI631051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGCA GGAACTCCCA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066567.1
CDS	20..346
Translation	MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARLGRQVDSMWAEQKQMELESILVALLQKHSRNSQG

Target ORF information:

RefSeq Version	NM_021081.5
Organism	Homo sapiens(human)
Definition	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021081.5

ORF Insert Sequence:

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGCA GGAACTCCCA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRH ( XM_011528788.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527090.1 Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu26804
Clone ID Related Accession (Same CDS sequence)	NM_001184731.2 , XM_011528788.2
Accession Version	XM_011528788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	somatoliberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGGA ACTCCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527090.1
CDS	95..418
Translation	MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARLGRQVDSMWAEQKQMELESILVALLQKHRNSQG

Target ORF information:

RefSeq Version	XM_011528788.2
Organism	Homo sapiens(human)
Definition	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528788.2

ORF Insert Sequence:

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGGA ACTCCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRH ( XM_011528784.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527086.1 Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu26946
Clone ID Related Accession (Same CDS sequence)	XM_011528784.2 , NM_021081.5
Accession Version	XM_011528784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	somatoliberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGCA GGAACTCCCA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527086.1
CDS	95..421
Translation	MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARLGRQVDSMWAEQKQMELESILVALLQKHSRNSQG

Target ORF information:

RefSeq Version	XM_011528784.2
Organism	Homo sapiens(human)
Definition	Homo sapiens growth hormone releasing hormone (GHRH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528784.2

ORF Insert Sequence:

ATGCCACTCT GGGTGTTCTT CTTTGTGATC CTCACCCTCA GCAACAGCTC CCACTGCTCC 
CCACCTCCCC CTTTGACCCT CAGGATGCGG CGGTATGCAG ATGCCATCTT CACCAACAGC 
TACCGGAAGG TGCTGGGCCA GCTGTCCGCC CGCAAGCTGC TCCAGGACAT CATGAGCAGG 
CAGCAGGGAG AGAGCAACCA AGAGCGAGGA GCAAGGGCAC GGCTTGGTCG TCAGGTAGAC 
AGCATGTGGG CAGAACAAAA GCAAATGGAA TTGGAGAGCA TCCTGGTGGC CCTGCTGCAG 
AAGCACAGCA GGAACTCCCA GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression-cloning and sequence of a cDNA encoding human growth hormone-releasing factor.
Nature306(5938)86-8(1983 Nov 3-9)
Mayo KE,Vale W,Rivier J,Rosenfeld MG,Evans RM

Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly.
Science (New York, N.Y.)218(4572)585-7(1982 Nov)
Guillemin R,Brazeau P,B?hlen P,Esch F,Ling N,Wehrenberg WB

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GHRH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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