AMFR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMFR
Entrez Gene ID	267
Full Name	autocrine motility factor receptor
Synonyms	GP78,RNF45
General protein information	Preferred Names autocrine motility factor receptor Names E3 ubiquitin-protein ligase AMFR RING finger protein 45 RING-type E3 ubiquitin transferase AMFR
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q13
Summary	This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012].
Disorder MIM:	603243

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144.5	NP_001135.3	E3 ubiquitin-protein ligase AMFR isoform c
XM_005255890.3	XP_005255947.1	E3 ubiquitin-protein ligase AMFR isoform X1
XM_005255890.4	XP_005255947.1	E3 ubiquitin-protein ligase AMFR isoform X1
NM_001144.6	NP_001135.3	E3 ubiquitin-protein ligase AMFR isoform c
NM_001323511.1	NP_001310440.1	E3 ubiquitin-protein ligase AMFR isoform e
NM_001323512.1	NP_001310441.1	E3 ubiquitin-protein ligase AMFR isoform d
NM_001323511.2	NP_001310440.1	E3 ubiquitin-protein ligase AMFR isoform e
NM_001323512.2	NP_001310441.1	E3 ubiquitin-protein ligase AMFR isoform d

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AMFR	XP_005617971.1
Rattus norvegicus (Norway rat)	Amfr	XP_006255201.1
Pan troglodytes (chimpanzee)	AMFR	XP_001135064.2
Danio rerio (zebrafish)	amfr	NP_998328.1
Xenopus tropicalis (tropical clawed frog)	amfr	NP_001017083.1
Gallus gallus (chicken)	AMFR	XP_414064.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007538	XP_001237062.2
Bos taurus (cattle)	AMFR	NP_001039439.1
Mus musculus (house mouse)	Amfr	NP_035917.2
Homo sapiens (human)	AMFR	NP_001135.3
Caenorhabditis elegans (roundworm)	hrdl-1	NP_492602.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of HSPA1L as a cellular prion protein stabilizer in tumor progression via HIF-1?/GP78 axis.
Lee JH, Han YS, Yoon YM, Yun CW, Yun SP, Kim SM, Kwon HY, Jeong D, Baek MJ, Lee HJ, Lee SJ, Han HJ, Lee SH
Oncogene36(47)6555-6567(2017 Nov)

SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78.
Khodayari N, Wang RL, Marek G, Krotova K, Kirst M, Liu C, Rouhani F, Brantly M
PloS one12(3)e0172983(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first study to show that HSPA1L mediated HIF-1alpha stabilization. In addition, this is the first study to show that GP78 inactivation promotes cancer cell proliferation, migration and eventual tumor growth both in vivo and in vitro by increasing cellular prion protein.
Title: Role of HSPA1L as a cellular prion protein stabilizer in tumor progression via HIF-1?/GP78 axis.

overexpression of gp78 or SVIP suppression may eliminate the toxic gain of function associated with polymerization of ZAAT, thus providing a potential new therapeutic approach to the treatment of alpha-1 antitrypsin deficiency
Title: SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78.

Despite its interaction with gp78, Lnp does not seem to have a broad function in degradation of misfolded ER proteins.
Title: Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions.

Further study discovered that the gp78 CUE domain works as a proofreading machine during the growth of K48-linked polyubiquitin chains to ensure the linkage specificity. Together, our studies uncover a novel mechanism underlying the linkage specificity determination of longer polyubiquitin chains.
Title: The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination.

Catalytic inactivation of MGRN1 results in elevated levels of GP78 and a consequential increase in the initiation of mitophagy.
Title: Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.

The following AMFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14318	NM_001144.6 Latest version!	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu49337	NM_001323511.2 Latest version!	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu49337	XM_005255890.4 Latest version!	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu101965	NM_001323512.2 Latest version!	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu49337	XM_005255890.3	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu14318	NM_001144.5	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu49337	NM_001323511.1	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu101965	NM_001323512.1	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMFR ( NM_001144.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135.3 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14318
Clone ID Related Accession (Same CDS sequence)	NM_001144.5 , NM_001144.6
Accession Version	NM_001144.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069197.1, BX345849.2, CA748782.1, AC009102.10, BX409690.2 and AA767223.1. This sequence is a reference standard in the RefSeqGene project. On Feb 28, 2012 this sequence version replaced NM_001144.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069197.1, SRR1803613.88703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGGTC TCGGATTGCG AGCTGGCTGC CGAGTTTTTC GGTTGAAGTG 
ATGCACACCA CCAACATTCT TGGCATTACG CAGGCCAGCA ACTCCCAGCT CAATGCAATG 
GCTCATCAGA TTCAAGAGAT GTTTCCCCAG GTTCCATACC ATCTGGTACT GCAGGACCTC 
CAGCTGACAC GCTCAGTTGA AATAACAACA GACAATATTT TAGAAGGACG GATTCAAGTA 
CCTTTTCCTA CACAGCGGTC AGATAGCATC AGACCTGCAT TGAACAGTCC TGTGGAAAGG 
CCAAGCAGTG ACCAGGAAGA GGGAGAAACT TCTGCTCAGA CCGAGCGTGT GCCACTGGAC 
CTCAGTCCTC GCCTGGAGGA GACGCTGGAC TTCGGCGAGG TGGAAGTGGA GCCCAGTGAG 
GTGGAAGACT TCGAGGCTCG TGGGAGCCGC TTCTCCAAGT CTGCTGATGA GAGACAGCGC 
ATGCTGGTGC AGCGTAAGGA CGAACTCCTC CAGCAAGCTC GCAAACGTTT CTTGAACAAA 
AGTTCTGAAG ATGATGCGGC CTCAGAGAGC TTCCTCCCCT CGGAAGGTGC GTCCTCTGAC 
CCCGTGACCC TGCGTCGAAG GATGCTGGCT GCCGCCGCGG AACGGAGGCT TCAGAAGCAG 
CAGACCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135.3
CDS	212..2143
Translation	MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001144.5
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144.5

ORF Insert Sequence:

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGGTC TCGGATTGCG AGCTGGCTGC CGAGTTTTTC GGTTGAAGTG 
ATGCACACCA CCAACATTCT TGGCATTACG CAGGCCAGCA ACTCCCAGCT CAATGCAATG 
GCTCATCAGA TTCAAGAGAT GTTTCCCCAG GTTCCATACC ATCTGGTACT GCAGGACCTC 
CAGCTGACAC GCTCAGTTGA AATAACAACA GACAATATTT TAGAAGGACG GATTCAAGTA 
CCTTTTCCTA CACAGCGGTC AGATAGCATC AGACCTGCAT TGAACAGTCC TGTGGAAAGG 
CCAAGCAGTG ACCAGGAAGA GGGAGAAACT TCTGCTCAGA CCGAGCGTGT GCCACTGGAC 
CTCAGTCCTC GCCTGGAGGA GACGCTGGAC TTCGGCGAGG TGGAAGTGGA GCCCAGTGAG 
GTGGAAGACT TCGAGGCTCG TGGGAGCCGC TTCTCCAAGT CTGCTGATGA GAGACAGCGC 
ATGCTGGTGC AGCGTAAGGA CGAACTCCTC CAGCAAGCTC GCAAACGTTT CTTGAACAAA 
AGTTCTGAAG ATGATGCGGC CTCAGAGAGC TTCCTCCCCT CGGAAGGTGC GTCCTCTGAC 
CCCGTGACCC TGCGTCGAAG GATGCTGGCT GCCGCCGCGG AACGGAGGCT TCAGAAGCAG 
CAGACCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( NM_001144.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135.3 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14318
Clone ID Related Accession (Same CDS sequence)	NM_001144.5 , NM_001144.6
Accession Version	NM_001144.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092140.4, BC069197.1, BX345849.2, CA748782.1, AC009102.10, BX409690.2 and AA767223.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001144.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.88703.1, SRR1803617.150525.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290649.10/ ENSP00000290649.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGGTC TCGGATTGCG AGCTGGCTGC CGAGTTTTTC GGTTGAAGTG 
ATGCACACCA CCAACATTCT TGGCATTACG CAGGCCAGCA ACTCCCAGCT CAATGCAATG 
GCTCATCAGA TTCAAGAGAT GTTTCCCCAG GTTCCATACC ATCTGGTACT GCAGGACCTC 
CAGCTGACAC GCTCAGTTGA AATAACAACA GACAATATTT TAGAAGGACG GATTCAAGTA 
CCTTTTCCTA CACAGCGGTC AGATAGCATC AGACCTGCAT TGAACAGTCC TGTGGAAAGG 
CCAAGCAGTG ACCAGGAAGA GGGAGAAACT TCTGCTCAGA CCGAGCGTGT GCCACTGGAC 
CTCAGTCCTC GCCTGGAGGA GACGCTGGAC TTCGGCGAGG TGGAAGTGGA GCCCAGTGAG 
GTGGAAGACT TCGAGGCTCG TGGGAGCCGC TTCTCCAAGT CTGCTGATGA GAGACAGCGC 
ATGCTGGTGC AGCGTAAGGA CGAACTCCTC CAGCAAGCTC GCAAACGTTT CTTGAACAAA 
AGTTCTGAAG ATGATGCGGC CTCAGAGAGC TTCCTCCCCT CGGAAGGTGC GTCCTCTGAC 
CCCGTGACCC TGCGTCGAAG GATGCTGGCT GCCGCCGCGG AACGGAGGCT TCAGAAGCAG 
CAGACCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135.3
CDS	219..2150
Translation	MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001144.6
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144.6

ORF Insert Sequence:

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGGTC TCGGATTGCG AGCTGGCTGC CGAGTTTTTC GGTTGAAGTG 
ATGCACACCA CCAACATTCT TGGCATTACG CAGGCCAGCA ACTCCCAGCT CAATGCAATG 
GCTCATCAGA TTCAAGAGAT GTTTCCCCAG GTTCCATACC ATCTGGTACT GCAGGACCTC 
CAGCTGACAC GCTCAGTTGA AATAACAACA GACAATATTT TAGAAGGACG GATTCAAGTA 
CCTTTTCCTA CACAGCGGTC AGATAGCATC AGACCTGCAT TGAACAGTCC TGTGGAAAGG 
CCAAGCAGTG ACCAGGAAGA GGGAGAAACT TCTGCTCAGA CCGAGCGTGT GCCACTGGAC 
CTCAGTCCTC GCCTGGAGGA GACGCTGGAC TTCGGCGAGG TGGAAGTGGA GCCCAGTGAG 
GTGGAAGACT TCGAGGCTCG TGGGAGCCGC TTCTCCAAGT CTGCTGATGA GAGACAGCGC 
ATGCTGGTGC AGCGTAAGGA CGAACTCCTC CAGCAAGCTC GCAAACGTTT CTTGAACAAA 
AGTTCTGAAG ATGATGCGGC CTCAGAGAGC TTCCTCCCCT CGGAAGGTGC GTCCTCTGAC 
CCCGTGACCC TGCGTCGAAG GATGCTGGCT GCCGCCGCGG AACGGAGGCT TCAGAAGCAG 
CAGACCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( NM_001323511.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310440.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu49337
Clone ID Related Accession (Same CDS sequence)	XM_005255890.3 , XM_005255890.4 , NM_001323511.1 , NM_001323511.2
Accession Version	NM_001323511.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092140.4, AA280077.1, CD642692.1, BQ889409.1, AC009102.10 and BC051032.1. On Apr 22, 2016 this sequence version replaced XM_005255889.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76953.1, SRR1803611.226988.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310440.1
CDS	116..1762
Translation	MLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001323511.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323511.1

ORF Insert Sequence:

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( NM_001323511.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310440.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu49337
Clone ID Related Accession (Same CDS sequence)	XM_005255890.3 , XM_005255890.4 , NM_001323511.1 , NM_001323511.2
Accession Version	NM_001323511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092140.4, AA280077.1, CD642692.1, BQ889409.1, AC009102.10 and BC051032.1. On May 31, 2019 this sequence version replaced NM_001323511.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.76953.1, SRR1803611.226988.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310440.1
CDS	110..1756
Translation	MLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001323511.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323511.2

ORF Insert Sequence:

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( XM_005255890.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255947.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu49337
Clone ID Related Accession (Same CDS sequence)	XM_005255890.3 , XM_005255890.4 , NM_001323511.1 , NM_001323511.2
Accession Version	XM_005255890.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255947.1
CDS	557..2203
Translation	MLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	XM_005255890.3
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255890.3

ORF Insert Sequence:

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( XM_005255890.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255947.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.

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CloneID	OHu49337
Clone ID Related Accession (Same CDS sequence)	XM_005255890.3 , XM_005255890.4 , NM_001323511.1 , NM_001323511.2
Accession Version	XM_005255890.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005255890.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255947.1
CDS	557..2203
Translation	MLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	XM_005255890.4
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255890.4

ORF Insert Sequence:

ATGTTGGTGG CTAAGCTCAT CCAGTGTATT GTGTTTGGCC CTCTTCGAGT GAGTGAGAGA 
CAGCATCTCA AAGACAAATT TTGGAATTTT ATTTTCTACA AGTTCATTTT CATCTTTGGT 
GTGCTGAATG TCCAGACAGT GGAAGAGGTG GTCATGTGGT GCCTCTGGTT TGCCGGACTT 
GTCTTTCTGC ACCTGATGGT TCAGCTCTGC AAGGATCGAT TTGAATATCT TTCCTTCTCG 
CCCACCACGC CGATGAGCAG CCACGGTCGA GTCCTGTCCC TGTTGGTTGC CATGCTGCTT 
TCCTGCTGTG GACTGGCGGC CGTCTGCTCC ATCACCGGCT ACACCCACGG AATGCACACC 
TTGGCTTTCA TGGCTGCAGA GTCTCTTCTT GTGACAGTGA GGACTGCTCA TGTGATTTTA 
CGATACGTAA TTCACCTCTG GGACCTCAAC CACGAAGGGA CGTGGGAAGG AAAGGGGACG 
TATGTCTATT ACACAGACTT TGTCATGGAG CTCACTCTCC TGTCCCTGGA CCTCATGCAC 
CATATTCACA TGTTGTTATT TGGCAACATC TGGTTATCCA TGGCCAGCCT GGTCATCTTT 
ATGCAGCTGC GTTACCTGTT TCATGAGGTG CAACGTCGAA TTCGTCGGCA CAAGAACTAT 
CTACGTGTGG TTGGAAACAT GGAGGCCAGG TTTGCAGTTG CAACTCCAGA GGAGCTGGCT 
GTCAACAATG ACGACTGTGC CATCTGTTGG GACTCCATGC AGGCTGCGCG GAAACTGCCC 
TGTGGACATC TTTTCCACAA CTCCTGTCTT CGTTCCTGGC TAGAACAAGA CACCTCCTGT 
CCAACATGCA GAATGTCTCT TAATATTGCC GACAATAATC GTGTCAGGGA AGAACATCAA 
GGAGAGAACT TGGATGAGAA TTTGGTTCCT GTAGCAGCAG CCGAAGGGAG ACCTCGCTTA 
AACCAACACA ATCACTTCTT CCATTTCGAT GGGTCTCGGA TTGCGAGCTG GCTGCCGAGT 
TTTTCGGTTG AAGTGATGCA CACCACCAAC ATTCTTGGCA TTACGCAGGC CAGCAACTCC 
CAGCTCAATG CAATGGCTCA TCAGATTCAA GAGATGTTTC CCCAGGTTCC ATACCATCTG 
GTACTGCAGG ACCTCCAGCT GACACGCTCA GTTGAAATAA CAACAGACAA TATTTTAGAA 
GGACGGATTC AAGTACCTTT TCCTACACAG CGGTCAGATA GCATCAGACC TGCATTGAAC 
AGTCCTGTGG AAAGGCCAAG CAGTGACCAG GAAGAGGGAG AAACTTCTGC TCAGACCGAG 
CGTGTGCCAC TGGACCTCAG TCCTCGCCTG GAGGAGACGC TGGACTTCGG CGAGGTGGAA 
GTGGAGCCCA GTGAGGTGGA AGACTTCGAG GCTCGTGGGA GCCGCTTCTC CAAGTCTGCT 
GATGAGAGAC AGCGCATGCT GGTGCAGCGT AAGGACGAAC TCCTCCAGCA AGCTCGCAAA 
CGTTTCTTGA ACAAAAGTTC TGAAGATGAT GCGGCCTCAG AGAGCTTCCT CCCCTCGGAA 
GGTGCGTCCT CTGACCCCGT GACCCTGCGT CGAAGGATGC TGGCTGCCGC CGCGGAACGG 
AGGCTTCAGA AGCAGCAGAC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( NM_001323512.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310441.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101965
Clone ID Related Accession (Same CDS sequence)	NM_001323512.1 , NM_001323512.2
Accession Version	NM_001323512.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069197.1, DR005354.1, DB172402.1, BI766313.1, AC009102.10, BC051032.1 and CA425340.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.133709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGAGA TGACAGCTCC ATGCGTGTTA CTGCCCCTGA AGACGTTCCA 
GTGGGACAGG AGGTGGAGGT GGTCGACAGT GATACTGATG CTCCTGACCT GTGGTCTCGG 
ATTGCGAGCT GGCTGCCGAG TTTTTCGGTT GAAGTGATGC ACACCACCAA CATTCTTGGC 
ATTACGCAGG CCAGCAACTC CCAGCTCAAT GCAATGGCTC ATCAGATTCA AGAGATGTTT 
CCCCAGGTTC CATACCATCT GGTACTGCAG GACCTCCAGC TGACACGCTC AGTTGAAATA 
ACAACAGACA ATATTTTAGA AGGACGGATT CAAGTACCTT TTCCTACACA GCGGTCAGAT 
AGCATCAGAC CTGCATTGAA CAGTCCTGTG GAAAGGCCAA GCAGTGACCA GGAAGAGGGA 
GAAACTTCTG CTCAGACCGA GCGTGTGCCA CTGGACCTCA GTCCTCGCCT GGAGGAGACG 
CTGGACTTCG GCGAGGTGGA AGTGGAGCCC AGTGAGGTGG AAGACTTCGA GGCTCGTGGG 
AGCCGCTTCT CCAAGTCTGC TGATGAGAGA CAGCGCATGC TGGTGCAGCG TAAGGACGAA 
CTCCTCCAGC AAGCTCGCAA ACGTTTCTTG AACAAAAGTT CTGAAGATGA TGCGGCCTCA 
GAGAGCTTCC TCCCCTCGGA AGGTGCGTCC TCTGACCCCG TGACCCTGCG TCGAAGGATG 
CTGGCTGCCG CCGCGGAACG GAGGCTTCAG AAGCAGCAGA CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310441.1
CDS	212..2239
Translation	MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGDDSSMRVTAPEDVPVGQEVEVVDSDTDAPDLWSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001323512.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323512.1

ORF Insert Sequence:

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGAGA TGACAGCTCC ATGCGTGTTA CTGCCCCTGA AGACGTTCCA 
GTGGGACAGG AGGTGGAGGT GGTCGACAGT GATACTGATG CTCCTGACCT GTGGTCTCGG 
ATTGCGAGCT GGCTGCCGAG TTTTTCGGTT GAAGTGATGC ACACCACCAA CATTCTTGGC 
ATTACGCAGG CCAGCAACTC CCAGCTCAAT GCAATGGCTC ATCAGATTCA AGAGATGTTT 
CCCCAGGTTC CATACCATCT GGTACTGCAG GACCTCCAGC TGACACGCTC AGTTGAAATA 
ACAACAGACA ATATTTTAGA AGGACGGATT CAAGTACCTT TTCCTACACA GCGGTCAGAT 
AGCATCAGAC CTGCATTGAA CAGTCCTGTG GAAAGGCCAA GCAGTGACCA GGAAGAGGGA 
GAAACTTCTG CTCAGACCGA GCGTGTGCCA CTGGACCTCA GTCCTCGCCT GGAGGAGACG 
CTGGACTTCG GCGAGGTGGA AGTGGAGCCC AGTGAGGTGG AAGACTTCGA GGCTCGTGGG 
AGCCGCTTCT CCAAGTCTGC TGATGAGAGA CAGCGCATGC TGGTGCAGCG TAAGGACGAA 
CTCCTCCAGC AAGCTCGCAA ACGTTTCTTG AACAAAAGTT CTGAAGATGA TGCGGCCTCA 
GAGAGCTTCC TCCCCTCGGA AGGTGCGTCC TCTGACCCCG TGACCCTGCG TCGAAGGATG 
CTGGCTGCCG CCGCGGAACG GAGGCTTCAG AAGCAGCAGA CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMFR ( NM_001323512.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310441.1 Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101965
Clone ID Related Accession (Same CDS sequence)	NM_001323512.1 , NM_001323512.2
Accession Version	NM_001323512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase AMFR isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092140.4, BC069197.1, DR005354.1, DB172402.1, BI766313.1, AC009102.10, BC051032.1 and CA425340.1. On May 31, 2019 this sequence version replaced NM_001323512.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.133709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGAGA TGACAGCTCC ATGCGTGTTA CTGCCCCTGA AGACGTTCCA 
GTGGGACAGG AGGTGGAGGT GGTCGACAGT GATACTGATG CTCCTGACCT GTGGTCTCGG 
ATTGCGAGCT GGCTGCCGAG TTTTTCGGTT GAAGTGATGC ACACCACCAA CATTCTTGGC 
ATTACGCAGG CCAGCAACTC CCAGCTCAAT GCAATGGCTC ATCAGATTCA AGAGATGTTT 
CCCCAGGTTC CATACCATCT GGTACTGCAG GACCTCCAGC TGACACGCTC AGTTGAAATA 
ACAACAGACA ATATTTTAGA AGGACGGATT CAAGTACCTT TTCCTACACA GCGGTCAGAT 
AGCATCAGAC CTGCATTGAA CAGTCCTGTG GAAAGGCCAA GCAGTGACCA GGAAGAGGGA 
GAAACTTCTG CTCAGACCGA GCGTGTGCCA CTGGACCTCA GTCCTCGCCT GGAGGAGACG 
CTGGACTTCG GCGAGGTGGA AGTGGAGCCC AGTGAGGTGG AAGACTTCGA GGCTCGTGGG 
AGCCGCTTCT CCAAGTCTGC TGATGAGAGA CAGCGCATGC TGGTGCAGCG TAAGGACGAA 
CTCCTCCAGC AAGCTCGCAA ACGTTTCTTG AACAAAAGTT CTGAAGATGA TGCGGCCTCA 
GAGAGCTTCC TCCCCTCGGA AGGTGCGTCC TCTGACCCCG TGACCCTGCG TCGAAGGATG 
CTGGCTGCCG CCGCGGAACG GAGGCTTCAG AAGCAGCAGA CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310441.1
CDS	219..2246
Translation	MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGDDSSMRVTAPEDVPVGQEVEVVDSDTDAPDLWSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

Target ORF information:

RefSeq Version	NM_001323512.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autocrine motility factor receptor (AMFR), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323512.2

ORF Insert Sequence:

ATGCCGCTGC TCTTCCTCGA GCGCTTCCCC TGGCCCAGCC TCCGCACCTA CACGGGCCTC 
AGCGGCCTGG CCCTGCTGGG CACCATCATC AGCGCCTACC GCGCGCTCAG CCAGCCCGAG 
GCCGGCCCCG GCGAGCCGGA CCAGCTAACG GCCTCGCTGC AGCCTGAGCC GCCGGCGCCC 
GCCCGGCCGA GCGCCGGGGG ACCCCGGGCC CGCGATGTGG CCCAGTACCT GCTCTCAGAC 
AGCCTCTTCG TGTGGGTTCT AGTAAATACC GCTTGCTGTG TTTTGATGTT GGTGGCTAAG 
CTCATCCAGT GTATTGTGTT TGGCCCTCTT CGAGTGAGTG AGAGACAGCA TCTCAAAGAC 
AAATTTTGGA ATTTTATTTT CTACAAGTTC ATTTTCATCT TTGGTGTGCT GAATGTCCAG 
ACAGTGGAAG AGGTGGTCAT GTGGTGCCTC TGGTTTGCCG GACTTGTCTT TCTGCACCTG 
ATGGTTCAGC TCTGCAAGGA TCGATTTGAA TATCTTTCCT TCTCGCCCAC CACGCCGATG 
AGCAGCCACG GTCGAGTCCT GTCCCTGTTG GTTGCCATGC TGCTTTCCTG CTGTGGACTG 
GCGGCCGTCT GCTCCATCAC CGGCTACACC CACGGAATGC ACACCTTGGC TTTCATGGCT 
GCAGAGTCTC TTCTTGTGAC AGTGAGGACT GCTCATGTGA TTTTACGATA CGTAATTCAC 
CTCTGGGACC TCAACCACGA AGGGACGTGG GAAGGAAAGG GGACGTATGT CTATTACACA 
GACTTTGTCA TGGAGCTCAC TCTCCTGTCC CTGGACCTCA TGCACCATAT TCACATGTTG 
TTATTTGGCA ACATCTGGTT ATCCATGGCC AGCCTGGTCA TCTTTATGCA GCTGCGTTAC 
CTGTTTCATG AGGTGCAACG TCGAATTCGT CGGCACAAGA ACTATCTACG TGTGGTTGGA 
AACATGGAGG CCAGGTTTGC AGTTGCAACT CCAGAGGAGC TGGCTGTCAA CAATGACGAC 
TGTGCCATCT GTTGGGACTC CATGCAGGCT GCGCGGAAAC TGCCCTGTGG ACATCTTTTC 
CACAACTCCT GTCTTCGTTC CTGGCTAGAA CAAGACACCT CCTGTCCAAC ATGCAGAATG 
TCTCTTAATA TTGCCGACAA TAATCGTGTC AGGGAAGAAC ATCAAGGAGA GAACTTGGAT 
GAGAATTTGG TTCCTGTAGC AGCAGCCGAA GGGAGACCTC GCTTAAACCA ACACAATCAC 
TTCTTCCATT TCGATGGAGA TGACAGCTCC ATGCGTGTTA CTGCCCCTGA AGACGTTCCA 
GTGGGACAGG AGGTGGAGGT GGTCGACAGT GATACTGATG CTCCTGACCT GTGGTCTCGG 
ATTGCGAGCT GGCTGCCGAG TTTTTCGGTT GAAGTGATGC ACACCACCAA CATTCTTGGC 
ATTACGCAGG CCAGCAACTC CCAGCTCAAT GCAATGGCTC ATCAGATTCA AGAGATGTTT 
CCCCAGGTTC CATACCATCT GGTACTGCAG GACCTCCAGC TGACACGCTC AGTTGAAATA 
ACAACAGACA ATATTTTAGA AGGACGGATT CAAGTACCTT TTCCTACACA GCGGTCAGAT 
AGCATCAGAC CTGCATTGAA CAGTCCTGTG GAAAGGCCAA GCAGTGACCA GGAAGAGGGA 
GAAACTTCTG CTCAGACCGA GCGTGTGCCA CTGGACCTCA GTCCTCGCCT GGAGGAGACG 
CTGGACTTCG GCGAGGTGGA AGTGGAGCCC AGTGAGGTGG AAGACTTCGA GGCTCGTGGG 
AGCCGCTTCT CCAAGTCTGC TGATGAGAGA CAGCGCATGC TGGTGCAGCG TAAGGACGAA 
CTCCTCCAGC AAGCTCGCAA ACGTTTCTTG AACAAAAGTT CTGAAGATGA TGCGGCCTCA 
GAGAGCTTCC TCCCCTCGGA AGGTGCGTCC TCTGACCCCG TGACCCTGCG TCGAAGGATG 
CTGGCTGCCG CCGCGGAACG GAGGCTTCAG AAGCAGCAGA CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of HSPA1L as a cellular prion protein stabilizer in tumor progression via HIF-1?/GP78 axis.
Oncogene36(47)6555-6567(2017 Nov)
Lee JH,Han YS,Yoon YM,Yun CW,Yun SP,Kim SM,Kwon HY,Jeong D,Baek MJ,Lee HJ,Lee SJ,Han HJ,Lee SH

SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78.
PloS one12(3)e0172983(2017)
Khodayari N,Wang RL,Marek G,Krotova K,Kirst M,Liu C,Rouhani F,Brantly M

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AMFR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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