GCK gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_033508.1	NP_277043.1	glucokinase isoform 3
NM_000162.4	NP_000153.1	glucokinase isoform 1
NM_001354800.1	NP_001341729.1	hexokinase-4 isoform 4
XM_017011966.1	XP_016867455.1	glucokinase isoform X1
NM_033508.2	NP_277043.1	glucokinase isoform 3
NM_001354801.1	NP_001341730.1	hexokinase-4 isoform 7
NM_001354802.1	NP_001341731.1	hexokinase-4 isoform 5
NM_000162.3	NP_000153.1	glucokinase isoform 1
NM_001354803.1	NP_001341732.1	hexokinase-4 isoform 6
XM_024446707.1	XP_024302475.1	hexokinase-4 isoform X1
NM_033507.1	NP_277042.1	glucokinase isoform 2
NM_033507.2	NP_277042.1	glucokinase isoform 2
NM_000162.5	NP_000153.1	hexokinase-4 isoform 1
NM_033507.3	NP_277042.1	hexokinase-4 isoform 2
NM_033508.3	NP_277043.1	hexokinase-4 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18369	NM_000162.5 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu103002	NM_001354802.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu17606	NM_033508.3 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu17640	NM_033507.3 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu97159	NM_001354800.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu97159	XM_017011966.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102979	NM_001354803.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu102989	NM_001354801.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu105877	XM_024446707.1 Latest version!	Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu17606	NM_033508.1	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu18369	NM_000162.3	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17640	NM_033507.1	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu18369	NM_000162.4	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17640	NM_033507.2	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu17606	NM_033508.2	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
Hide Old Accession Versions >>

GCK ( NM_000162.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000153.1 Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18369
Clone ID Related Accession (Same CDS sequence)	NM_000162.4 , NM_000162.3 , NM_000162.5
Accession Version	NM_000162.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	glucokinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4, BC001890.1 and M90299.1. On Aug 29, 2017 this sequence version replaced NM_000162.3. Transcript Variant: This variant (1) encodes the isoform expressed specifically in pancreatic islet beta cells. Its first exon is specific to this variant, which has a unique 5' UTR. Isoform 1 has a distinct N-terminus; the remainder of the protein is identical to isoforms 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M90299.1, BC001890.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000153.1
CDS	487..1884
Translation	MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_000162.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000162.4

ORF Insert Sequence:

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_000162.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000153.1 Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18369
Clone ID Related Accession (Same CDS sequence)	NM_000162.4 , NM_000162.3 , NM_000162.5
Accession Version	NM_000162.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Homo sapiens(Human)
Product	glucokinase isoform 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001890.1 and M90299.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2008 this sequence version replaced NM_000162.2. Transcript Variant: This variant (1) encodes the isoform expressed specifically in pancreatic islet beta cells. Its first exon is specific to this variant, which has a unique 5' UTR. Isoform 1 has a distinct N-terminus; the remainder of the protein is identical to isoforms 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001890.1, SRR1803616.110638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000153.1
CDS	471..1868
Translation	MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_000162.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000162.3

ORF Insert Sequence:

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_000162.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000153.1 Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18369
Clone ID Related Accession (Same CDS sequence)	NM_000162.4 , NM_000162.3 , NM_000162.5
Accession Version	NM_000162.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4 and BC001890.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000162.4. Transcript Variant: This variant (1) encodes the isoform expressed specifically in pancreatic islet beta cells. Its first exon is specific to this variant, which has a unique 5' UTR. Isoform 1 has a distinct N-terminus; the remainder of the protein is identical to isoforms 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001890.1, M90299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000403799.8/ ENSP00000384247.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000153.1
CDS	487..1884
Translation	MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_000162.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000162.5

ORF Insert Sequence:

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_001354802.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341731.1 Homo sapiens glucokinase (GCK), transcript variant 5, mRNA.

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CloneID	OHu103002
Clone ID Related Accession (Same CDS sequence)	NM_001354802.1
Accession Version	NM_001354802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.300314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341731.1
CDS	358..588
Translation	MCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVA

Target ORF information:

RefSeq Version	NM_001354802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354802.1

ORF Insert Sequence:

ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033508.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_277043.1 Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

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CloneID	OHu17606
Clone ID Related Accession (Same CDS sequence)	NM_033508.1 , NM_033508.2 , NM_033508.3
Accession Version	NM_033508.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Homo sapiens(Human)
Product	glucokinase isoform 3
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4, M69051.1, DA640823.1, CD251038.1 and M90299.1. Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M69051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277043.1
CDS	299..1693
Translation	MPRPRSQLPQPNSQVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033508.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033508.1

ORF Insert Sequence:

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033508.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_277043.1 Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

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CloneID	OHu17606
Clone ID Related Accession (Same CDS sequence)	NM_033508.1 , NM_033508.2 , NM_033508.3
Accession Version	NM_033508.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	glucokinase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122876.1, M69051.1, DA640823.1, CD251038.1 and M90299.1. On Aug 24, 2017 this sequence version replaced NM_033508.1. Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M69051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277043.1
CDS	293..1687
Translation	MPRPRSQLPQPNSQVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033508.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033508.2

ORF Insert Sequence:

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033508.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_277043.1 Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

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CloneID	OHu17606
Clone ID Related Accession (Same CDS sequence)	NM_033508.1 , NM_033508.2 , NM_033508.3
Accession Version	NM_033508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122876.1, M69051.1, DA640823.1, CD251038.1 and M90299.1. On May 31, 2019 this sequence version replaced NM_033508.2. Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M69051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277043.1
CDS	293..1687
Translation	MPRPRSQLPQPNSQVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033508.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033508.3

ORF Insert Sequence:

ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG 
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC 
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC 
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG 
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG 
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC 
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG 
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC 
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA 
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG 
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG 
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG 
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT 
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG 
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC 
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC 
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC 
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG 
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA 
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG 
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG 
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT 
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033507.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_277042.1 Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

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CloneID	OHu17640
Clone ID Related Accession (Same CDS sequence)	NM_033507.1 , NM_033507.2 , NM_033507.3
Accession Version	NM_033507.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Homo sapiens(Human)
Product	glucokinase isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4, AK122876.1, M69051.1, CD251038.1 and M90299.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277042.1
CDS	169..1569
Translation	MAMDVTRSQAQTALTLVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033507.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033507.1

ORF Insert Sequence:

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033507.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_277042.1 Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

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CloneID	OHu17640
Clone ID Related Accession (Same CDS sequence)	NM_033507.1 , NM_033507.2 , NM_033507.3
Accession Version	NM_033507.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	glucokinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122876.1, M69051.1, CD251038.1 and M90299.1. On Aug 24, 2017 this sequence version replaced NM_033507.1. Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277042.1
CDS	163..1563
Translation	MAMDVTRSQAQTALTLVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033507.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033507.2

ORF Insert Sequence:

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_033507.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_277042.1 Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

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CloneID	OHu17640
Clone ID Related Accession (Same CDS sequence)	NM_033507.1 , NM_033507.2 , NM_033507.3
Accession Version	NM_033507.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122876.1, M69051.1, CD251038.1 and M90299.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_033507.2. Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277042.1
CDS	163..1563
Translation	MAMDVTRSQAQTALTLVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_033507.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033507.3

ORF Insert Sequence:

ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG 
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC 
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG 
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG 
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT 
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG 
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC 
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC 
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG 
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG 
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG 
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC 
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG 
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC 
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG 
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG 
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC 
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG 
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG 
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC 
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC 
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG 
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_001354800.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341729.1 Homo sapiens glucokinase (GCK), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu97159
Clone ID Related Accession (Same CDS sequence)	NM_001354800.1 , XM_017011966.1
Accession Version	NM_001354800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4. On Aug 24, 2017 this sequence version replaced XM_017011966.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.205900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341729.1
CDS	487..1857
Translation	MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVA

Target ORF information:

RefSeq Version	NM_001354800.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354800.1

ORF Insert Sequence:

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( XM_017011966.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867455.1 Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.

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CloneID	OHu97159
Clone ID Related Accession (Same CDS sequence)	NM_001354800.1 , XM_017011966.1
Accession Version	XM_017011966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	glucokinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867455.1
CDS	487..1857
Translation	MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVA

Target ORF information:

RefSeq Version	XM_017011966.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011966.1

ORF Insert Sequence:

ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG 
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC 
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT 
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC 
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG 
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA 
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT 
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT 
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG 
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC 
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT 
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC 
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC 
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG 
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC 
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC 
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG 
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC 
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_001354803.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341732.1 Homo sapiens glucokinase (GCK), transcript variant 6, mRNA.

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CloneID	OHu102979
Clone ID Related Accession (Same CDS sequence)	NM_001354803.1
Accession Version	NM_001354803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4 and M90299.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI767691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267757 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAAGAG CAGGGATGGG AGCGAGCCTG CGTCCGGGCA CTGGTCCCCA TCCCGACACG 
GGCGACCGCA AGCAGATCTA CAACATCCTG AGCACGCTGG GGCTGCGACC CTCGACCACC 
GACTGCGACA TCGTGCGCCG CGCCTGCGAG AGCGTGTCTA CGCGCGCTGC GCACATGTGC 
TCGGCGGGGC TGGCGGGCGT CATCAACCGC ATGCGCGAGA GCCGCAGCGA GGACGTAATG 
CGCATCACTG TGGGCGTGGA TGGCTCCGTG TACAAGCTGC ACCCCAGCTT CAAGGAGCGG 
TTCCATGCCA GCGTGCGCAG GCTGACGCCC AGCTGCGAGA TCACCTTCAT CGAGTCGGAG 
GAGGGCAGTG GCCGGGGCGC GGCCCTGGTC TCGGCGGTGG CCTGTAAGAA GGCCTGTATG 
CTGGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341732.1
CDS	19..450
Translation	MPRAGMGASLRPGTGPHPDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_001354803.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354803.1

ORF Insert Sequence:

ATGCCAAGAG CAGGGATGGG AGCGAGCCTG CGTCCGGGCA CTGGTCCCCA TCCCGACACG 
GGCGACCGCA AGCAGATCTA CAACATCCTG AGCACGCTGG GGCTGCGACC CTCGACCACC 
GACTGCGACA TCGTGCGCCG CGCCTGCGAG AGCGTGTCTA CGCGCGCTGC GCACATGTGC 
TCGGCGGGGC TGGCGGGCGT CATCAACCGC ATGCGCGAGA GCCGCAGCGA GGACGTAATG 
CGCATCACTG TGGGCGTGGA TGGCTCCGTG TACAAGCTGC ACCCCAGCTT CAAGGAGCGG 
TTCCATGCCA GCGTGCGCAG GCTGACGCCC AGCTGCGAGA TCACCTTCAT CGAGTCGGAG 
GAGGGCAGTG GCCGGGGCGC GGCCCTGGTC TCGGCGGTGG CCTGTAAGAA GGCCTGTATG 
CTGGGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( NM_001354801.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341730.1 Homo sapiens glucokinase (GCK), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu102989
Clone ID Related Accession (Same CDS sequence)	NM_001354801.1
Accession Version	NM_001354801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4 and M90299.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ424932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125, SAMEA2162568 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCG ACACGGGCGA CCGCAAGCAG ATCTACAACA TCCTGAGCAC GCTGGGGCTG 
CGACCCTCGA CCACCGACTG CGACATCGTG CGCCGCGCCT GCGAGAGCGT GTCTACGCGC 
GCTGCGCACA TGTGCTCGGC GGGGCTGGCG GGCGTCATCA ACCGCATGCG CGAGAGCCGC 
AGCGAGGACG TAATGCGCAT CACTGTGGGC GTGGATGGCT CCGTGTACAA GCTGCACCCC 
AGCTTCAAGG AGCGGTTCCA TGCCAGCGTG CGCAGGCTGA CGCCCAGCTG CGAGATCACC 
TTCATCGAGT CGGAGGAGGG CAGTGGCCGG GGCGCGGCCC TGGTCTCGGC GGTGGCCTGT 
AAGAAGGCCT GTATGCTGGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341730.1
CDS	8..394
Translation	MSSDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	NM_001354801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354801.1

ORF Insert Sequence:

ATGTCCAGCG ACACGGGCGA CCGCAAGCAG ATCTACAACA TCCTGAGCAC GCTGGGGCTG 
CGACCCTCGA CCACCGACTG CGACATCGTG CGCCGCGCCT GCGAGAGCGT GTCTACGCGC 
GCTGCGCACA TGTGCTCGGC GGGGCTGGCG GGCGTCATCA ACCGCATGCG CGAGAGCCGC 
AGCGAGGACG TAATGCGCAT CACTGTGGGC GTGGATGGCT CCGTGTACAA GCTGCACCCC 
AGCTTCAAGG AGCGGTTCCA TGCCAGCGTG CGCAGGCTGA CGCCCAGCTG CGAGATCACC 
TTCATCGAGT CGGAGGAGGG CAGTGGCCGG GGCGCGGCCC TGGTCTCGGC GGTGGCCTGT 
AAGAAGGCCT GTATGCTGGG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCK ( XM_024446707.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302475.1 Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu105877
Clone ID Related Accession (Same CDS sequence)	XM_024446707.1
Accession Version	XM_024446707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hexokinase-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302475.1
CDS	341..598
Translation	MCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

Target ORF information:

RefSeq Version	XM_024446707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucokinase (GCK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446707.1

ORF Insert Sequence:

ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC 
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG 
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG 
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC 
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GCK
Entrez Gene ID	2645
Full Name	glucokinase
Synonyms	FGQTL3,GK,GLK,HHF3,HK4,HKIV,HXKP,LGLK,MODY2
General protein information	Preferred Names glucokinase Names glucokinase ATP:D-hexose 6-phosphotransferase HK IV glucokinase (hexokinase 4) hexokinase D, pancreatic isozyme hexokinase type IV
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p13
Summary	This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017].
Disorder MIM:	138079
Disorder Html:	MODY, type II, 125851 (3); Diabetes mellitus, noninsulin-dependent, late onset, 125853 (3); Hyperinsulinemic hypoglycemia, familial, 3, 602485 (3); Diabetes mellitus, permanent neonatal, 606176 (3)

	GCK	XP_001093035.2
Bos taurus (cattle)	GCK	NP_001095772.1
Xenopus tropicalis (tropical clawed frog)	gck	NP_001096321.1
Mus musculus (house mouse)	Gck	NP_034422.2
Rattus norvegicus (Norway rat)	Gck	NP_036697.1
Gallus gallus (chicken)	GCK	XP_427930.4
Homo sapiens (human)	GCK	NP_000153.1
Pan troglodytes (chimpanzee)	GCK	XP_001143302.1
Danio rerio (zebrafish)	gck	NP_001038850.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GCK cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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