GCDH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCDH
Entrez Gene ID	2639
Full Name	glutaryl-CoA dehydrogenase
Synonyms	ACAD5,GCD
General protein information	Preferred Names glutaryl-CoA dehydrogenase Names glutaryl-CoA dehydrogenase, mitochondrial glutaryl-Coenzyme A dehydrogenase truncated GCDH truncated glutaryl-CoA dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12. [provided by RefSeq, Mar 2013].
Disorder MIM:	608801
Disorder Html:	Glutaricaciduria, type I, 231670 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527899.2	XP_011526201.1	glutaryl-CoA dehydrogenase, mitochondrial isoform X1
XM_011527900.2	XP_011526202.1	glutaryl-CoA dehydrogenase, mitochondrial isoform X2
NM_013976.4	NP_039663.1	glutaryl-CoA dehydrogenase, mitochondrial isoform b precursor
NM_013976.3	NP_039663.1	glutaryl-CoA dehydrogenase, mitochondrial isoform b precursor
NM_000159.4	NP_000150.1	glutaryl-CoA dehydrogenase, mitochondrial isoform a precursor
XM_017026580.1	XP_016882069.1	glutaryl-CoA dehydrogenase, mitochondrial isoform X3
XM_006722721.2	XP_006722784.1	glutaryl-CoA dehydrogenase, mitochondrial isoform X4
NM_000159.3	NP_000150.1	glutaryl-CoA dehydrogenase, mitochondrial isoform a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCDH	NP_000150.1
	GCDH	XP_001110430.1
Mus musculus (house mouse)	Gcdh	NP_032123.3
Canis lupus familiaris (dog)	GCDH	XP_533901.2
Danio rerio (zebrafish)	gcdhl	NP_001006024.1
Drosophila melanogaster (fruit fly)	CG9547	NP_609040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008501	XP_316942.3
Bos taurus (cattle)	GCDH	NP_001039404.1
Rattus norvegicus (Norway rat)	Gcdh	NP_001102366.1
Magnaporthe oryzae (rice blast fungus)	MGG_06561	XP_003716979.1
Oryza sativa (rice)	Os01g0159400	NP_001042084.1
Danio rerio (zebrafish)	gcdh	NP_957180.1
Caenorhabditis elegans (roundworm)	CELE_F54D5.7	NP_496469.1
Neurospora crassa	NCU02291	XP_959935.1
Arabidopsis thaliana (thale cress)	ACX4	NP_190752.1
Oryza sativa (rice)	Os05g0163700	NP_001054738.1
Xenopus tropicalis (tropical clawed frog)	gcdh	NP_001016289.1
Pan troglodytes (chimpanzee)	GCDH	XP_001170352.2
Gallus gallus (chicken)	GCDH	XP_001231726.3

Gene Ontology
Bibliography

Related articles in PubMed

Genotype-phenotype correlation in 18 Egyptian patients with glutaric acidemia type I.
Mosaeilhy A, Mohamed MM, C GPD, El Abd HSA, Gamal R, Zaki OK, Zayed H
Metabolic brain disease32(5)1417-1426(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data underscore the impact of GCDH protein interactions mediated by amino acid residues on the surface of GCDH required for proper enzymatic activity
Title: Disease-causing mutations affecting surface residues of mitochondrial glutaryl-CoA dehydrogenase impair stability, heteromeric complex formation and mitochondria architecture.

We report the allele frequencies for three known Glutaric aciduria type I low excretors GCDH variants (M405V, V400M and R227P) and note that both the M405V and V400M variants are significantly more common in the population of African ancestry compared to the general population
Title: The M405V allele of the glutaryl-CoA dehydrogenase gene is an important marker for glutaric aciduria type I (GA-I) low excretors.

Mutations in GCDH gene observed in the present study indicate genetic heterogeneity in GCDH gene among South Indian population. No definite genotype-phenotype correlations were observed.
Title: Spectrum of mutations in Glutaryl-CoA dehydrogenase gene in glutaric aciduria type I--Study from South India.

Point mutation of GCDH gene is associated with glutaric academia type I.
Title: [Mutation analysis of GCDH gene in four patients with glutaric academia type I].

Data indicate a homozygous c.1244-2A> C mutation of the glutaryl-CoA dehydrogenase (GCDH) gene in both patients.
Title: [Clinical investigation and genetic analysis of a Chinese family with glutaric acidemia type I].

The following GCDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27620	NM_000159.4 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25206	NM_013976.4 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OHu51543	XM_011527900.2 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu51544	XM_006722721.2 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu51544	XM_017026580.1 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu57938	XM_011527899.2 Latest version!	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu25206	NM_013976.3	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OHu27620	NM_000159.3	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCDH ( NM_000159.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000150.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27620
Clone ID Related Accession (Same CDS sequence)	NM_000159.4 , NM_000159.3
Accession Version	NM_000159.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290407.1, U69141.1 and BM855466.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000159.3. Transcript Variant: This variant (1) encodes the longer isoform (a, also known as the long isoform). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KR674114.1, KT996148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000222214.10/ ENSP00000222214.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTACACA TGACATTCAC 
GCCCTGATCC TTGGGAGAGC TATCACGGGA ATCCAGGCGT TCACGGCCAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000150.1
CDS	78..1394
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Target ORF information:

RefSeq Version	NM_000159.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000159.4

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTACACA TGACATTCAC 
GCCCTGATCC TTGGGAGAGC TATCACGGGA ATCCAGGCGT TCACGGCCAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( NM_000159.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000150.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27620
Clone ID Related Accession (Same CDS sequence)	NM_000159.4 , NM_000159.3
Accession Version	NM_000159.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA286320.1, AK290407.1, U69141.1 and BM855466.1. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2013 this sequence version replaced NM_000159.2. Transcript Variant: This variant (1) encodes the longer isoform (a, also known as the long isoform). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KR674114.1, KU641459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTACACA TGACATTCAC 
GCCCTGATCC TTGGGAGAGC TATCACGGGA ATCCAGGCGT TCACGGCCAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000150.1
CDS	109..1425
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Target ORF information:

RefSeq Version	NM_000159.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000159.3

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTACACA TGACATTCAC 
GCCCTGATCC TTGGGAGAGC TATCACGGGA ATCCAGGCGT TCACGGCCAG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( NM_013976.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_039663.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25206
Clone ID Related Accession (Same CDS sequence)	NM_013976.4 , NM_013976.3
Accession Version	NM_013976.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290407.1, U69141.1 and BM855466.1. On May 31, 2019 this sequence version replaced NM_013976.3. Transcript Variant: This variant (2) lacks a segment that results in a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (b, also known as the short isoform) has a distinct and shorter C-terminus, compared to isoform a. This variant lacks publicly available transcript support but is supported by data in PubMed ID:8541831. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.574059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGTCGTTCA GATGTGTTCC 
TTAAAAAGAA GATGGAATTC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_039663.1
CDS	78..1364
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEVVQMCSLKRRWNSL

Target ORF information:

RefSeq Version	NM_013976.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013976.4

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGTCGTTCA GATGTGTTCC 
TTAAAAAGAA GATGGAATTC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( NM_013976.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_039663.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.

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CloneID	OHu25206
Clone ID Related Accession (Same CDS sequence)	NM_013976.4 , NM_013976.3
Accession Version	NM_013976.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA286320.1, AK290407.1, U69141.1 and BM855466.1. On Mar 13, 2013 this sequence version replaced NM_013976.2. Transcript Variant: This variant (2) lacks a segment that results in a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (b, also known as the short isoform) has a distinct and shorter C-terminus, compared to isoform a. This variant lacks publicly available transcript support but is supported by data in PubMed ID:8541831. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.574059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGTCGTTCA GATGTGTTCC 
TTAAAAAGAA GATGGAATTC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_039663.1
CDS	109..1395
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEVVQMCSLKRRWNSL

Target ORF information:

RefSeq Version	NM_013976.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013976.3

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGTCGTTCA GATGTGTTCC 
TTAAAAAGAA GATGGAATTC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( XM_011527900.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526202.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X2, mRNA.

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CloneID	OHu51543
Clone ID Related Accession (Same CDS sequence)	XM_011527900.2
Accession Version	XM_011527900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526202.1
CDS	78..1409
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEELLIQQWDMTTVWLLSSDTPCYRGSQTRW

Target ORF information:

RefSeq Version	XM_011527900.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527900.2

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( XM_017026580.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882069.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu51544
Clone ID Related Accession (Same CDS sequence)	XM_017026580.1 , XM_006722721.2
Accession Version	XM_017026580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882069.1
CDS	78..1406
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEELLIQQWDMTTVWLLSSDTPCYRGSQTR

Target ORF information:

RefSeq Version	XM_017026580.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026580.1

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( XM_006722721.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722784.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X4, mRNA.

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CloneID	OHu51544
Clone ID Related Accession (Same CDS sequence)	XM_017026580.1 , XM_006722721.2
Accession Version	XM_006722721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006722721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722784.1
CDS	78..1406
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEELLIQQWDMTTVWLLSSDTPCYRGSQTR

Target ORF information:

RefSeq Version	XM_006722721.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722721.2

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGAGCTGCT CATACAACAG 
TGGGACATGA CAACGGTTTG GCTTCTCAGC TCAGACACAC CATGTTATAG GGGATCACAA 
ACAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCDH ( XM_011527899.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526201.1 Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X1, mRNA.

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CloneID	OHu57938
Clone ID Related Accession (Same CDS sequence)	XM_011527899.2
Accession Version	XM_011527899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutaryl-CoA dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTGAGCT CATTCATTCC 
ACAGCTGCTG CTGAAACAAT GACTCCCACA GAACCATCTC CAGACCCTCA CCCCACCCCG 
AGTTCCGGGT CAGAAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526201.1
CDS	78..1418
Translation	MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGELIHSTAAAETMTPTEPSPDPHPTPSSGSES

Target ORF information:

RefSeq Version	XM_011527899.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaryl-CoA dehydrogenase (GCDH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527899.2

ORF Insert Sequence:

ATGGCCCTGA GAGGCGTCTC CGTGCGGCTG CTGAGCCGCG GACCCGGCCT GCACGTCCTT 
CGCACGTGGG TCTCGTCGGC GGCGCAGACC GAGAAAGGCG GGAGAACACA GAGCCAACTG 
GCTAAGTCCT CGCGTCCCGA GTTTGACTGG CAGGACCCGC TGGTGCTGGA GGAGCAGCTG 
ACCACAGATG AGATCCTCAT CAGGGACACC TTCCGCACCT ACTGCCAGGA GAGACTCATG 
CCTCGCATCC TGTTGGCCAA TCGCAACGAA GTTTTTCATC GGGAGATCAT TTCGGAGATG 
GGGGAGTTGG GTGTGCTGGG CCCCACCATC AAAGGATATG GCTGTGCTGG GGTTTCGTCT 
GTGGCCTATG GGCTCCTGGC CCGAGAGCTG GAGCGGGTGG ACAGTGGCTA CAGGTCGGCG 
ATGAGTGTCC AGTCCTCCCT CGTCATGCAC CCTATCTATG CCTATGGCAG CGAGGAACAG 
CGGCAGAAGT ACCTGCCCCA GCTGGCCAAG GGGGAGCTCC TGGGCTGCTT CGGGCTCACA 
GAGCCCAACA GCGGAAGTGA CCCCAGCAGC ATGGAGACCA GAGCCCACTA CAACTCATCC 
AACAAGAGCT ACACCCTCAA TGGGACCAAG ACCTGGATCA CGAACTCGCC TATGGCCGAT 
CTGTTTGTAG TGTGGGCTCG GTGTGAAGAT GGCTGCATTC GGGGCTTCCT GCTGGAGAAG 
GGGATGCGGG GTCTCTCGGC CCCCAGGATC CAGGGCAAGT TCTCGCTGCG GGCCTCAGCC 
ACAGGCATGA TCATCATGGA CGGTGTGGAG GTGCCAGAGG AGAATGTGCT CCCTGGTGCA 
TCCAGCCTGG GGGGTCCCTT CGGCTGCCTG AACAACGCCC GGTACGGCAT CGCGTGGGGC 
GTGCTTGGAG CTTCGGAGTT CTGCTTGCAC ACAGCCCGGC AGTACGCCCT CGACAGGATG 
CAGTTTGGTG TCCCACTGGC CAGGAACCAG CTGATTCAGA AGAAGCTGGC AGACATGCTC 
ACTGAGATTA CCCTGGGCCT TCACGCCTGC CTGCAGCTCG GCCGCTTGAA GGACCAGGAC 
AAGGCTGCCC CCGAGATGGT TTCTCTGCTG AAGAGGAATA ACTGTGGGAA AGCCCTGGAC 
ATCGCCCGCC AGGCCCGAGA CATGCTGGGG GGGAATGGGA TTTCTGACGA GTATCACGTG 
ATCCGGCACG CCATGAACCT GGAGGCCGTG AACACCTACG AAGGTGAGCT CATTCATTCC 
ACAGCTGCTG CTGAAACAAT GACTCCCACA GAACCATCTC CAGACCCTCA CCCCACCCCG 
AGTTCCGGGT CAGAAAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genotype-phenotype correlation in 18 Egyptian patients with glutaric acidemia type I.
Metabolic brain disease32(5)1417-1426(2017 Oct)
Mosaeilhy A,Mohamed MM,C GPD,El Abd HSA,Gamal R,Zaki OK,Zayed H

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GCDH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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