HSPB8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPB8
Entrez Gene ID	26353
Full Name	heat shock protein family B (small) member 8
Synonyms	CMT2L,DHMN2,E2IG1,H11,HMN2,HMN2A,HSP22
General protein information	Preferred Names heat shock protein family B (small) member 8 Names heat shock protein beta-8 E2-induced gene 1 protein alpha-crystallin C chain heat shock 22kDa protein 8 heat shock 27kDa protein 8 protein kinase H11 small stress protein-like protein HSP22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.23
Summary	The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008].
Disorder MIM:	608014
Disorder Html:	Neuropathy, distal hereditary motor, type IIA, 158590 (3); Charcot-Marie-Tooth disease, axonal, type 2L, 608673 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014365.2	NP_055180.1	heat shock protein beta-8
NM_014365.3	NP_055180.1	heat shock protein beta-8

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	hspb8	NP_001094427.2
Homo sapiens (human)	HSPB8	NP_055180.1
Pan troglodytes (chimpanzee)	HSPB8	XP_509417.1
Canis lupus familiaris (dog)	HSPB8	NP_001003029.1
Xenopus tropicalis (tropical clawed frog)	hspb8	NP_001005658.1
Mus musculus (house mouse)	Hspb8	NP_109629.1
Gallus gallus (chicken)	HSPB8	XP_415280.1
Bos taurus (cattle)	HSPB8	NP_001014955.1
	HSPB8	NP_001028109.1
Rattus norvegicus (Norway rat)	Hspb8	NP_446064.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered TDP-43-dependent splicing in HSPB8-related distal hereditary motor neuropathy and myofibrillar myopathy.
Cortese A, Laur? M, Casali C, Nishino I, Hayashi YK, Magri S, Taroni F, Stuani C, Saveri P, Moggio M, Ripolone M, Prelle A, Pisciotta C, Sagnelli A, Pichiecchio A, Reilly MM, Buratti E, Pareyson D
European journal of neurology25(1)154-163(2018 Jan)

Heat shock proteins HSPB8 and DNAJC5B have HCV antiviral activity.
Braga ACS, Carneiro BM, Batista MN, Akinaga MM, Bittar C, Rahal P
PloS one12(11)e0188467(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggest that HSPB8 may act as an intracellular factor against hepatitis C virus replication and that DNAJC5B has the same function, with more relevant results for genotype 3
Title: Heat shock proteins HSPB8 and DNAJC5B have HCV antiviral activity.

We report that overexpression of HSPB8 in immortalized motor neurones decreased the accumulation of TDP-25 and TDP-35 and that protection against mislocalized/truncated TDP-43 was observed for HSPB8 in Drosophila melanogaster Overexpression of HSP67Bc, the functional ortholog of human HSPB8, suppressed the eye degeneration caused by the cytoplasmic accumulation of a TDP-43 variant
Title: The chaperone HSPB8 reduces the accumulation of truncated TDP-43 species in cells and protects against TDP-43-mediated toxicity.

It has been demonstrated that HSPB8-BAG3-HSP70 ensures the functionality of stress granules and restores proteostasis by targeting defective ribosomal products for degradation.
Title: A Surveillance Function of the HSPB8-BAG3-HSP70 Chaperone Complex Ensures Stress Granule Integrity and Dynamism.

HSPB8 counteracts accumulation of aberrantly localized misfolded forms of TDP-43 and its 25 KDa fragment involved in most sporadic cases of Amyotrophic Lateral Sclerosis and of Fronto Lateral Temporal Dementia.
Title: Transcriptional induction of the heat shock protein B8 mediates the clearance of misfolded proteins responsible for motor neuron diseases.

expands the understanding of disease mechanisms, tissue involvement, and phenotypic outcome of HSPB8 mutations
Title: Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy.

The following HSPB8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPB8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26028	NM_014365.3 Latest version!	Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26028	NM_014365.2	Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPB8 ( NM_014365.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055180.1 Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.

Price & Availability↑

CloneID	OHu26028
Clone ID Related Accession (Same CDS sequence)	NM_014365.2 , NM_014365.3
Accession Version	NM_014365.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	heat shock protein beta-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF191017.1, BC002673.2 and AF217987.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 13, 2008 this sequence version replaced XR_019602.2, XR_017379.2, XR_038402.1, NM_014365.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF191017.1, SRR1660809.146443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACG GTCAGATGCC CTTCTCCTGC CACTACCCAA GCCGCCTGCG CCGAGACCCC 
TTCCGGGACT CTCCCCTCTC CTCTCGCCTG CTGGATGATG GCTTTGGCAT GGACCCCTTC 
CCAGACGACT TGACAGCCTC TTGGCCCGAC TGGGCTCTGC CTCGTCTCTC CTCCGCCTGG 
CCAGGCACCC TAAGGTCGGG CATGGTGCCC CGGGGCCCCA CTGCCACCGC CAGGTTTGGG 
GTGCCTGCCG AGGGCAGGAC CCCCCCACCC TTCCCTGGGG AGCCCTGGAA AGTGTGTGTG 
AATGTGCACA GCTTCAAGCC AGAGGAGTTG ATGGTGAAGA CCAAAGATGG ATACGTGGAG 
GTGTCTGGCA AACATGAAGA GAAACAGCAA GAAGGTGGCA TTGTTTCTAA GAACTTCACA 
AAGAAAATCC AGCTTCCTGC AGAGGTGGAT CCTGTGACAG TATTTGCCTC ACTTTCCCCA 
GAGGGTCTGC TGATCATCGA AGCTCCCCAG GTCCCTCCTT ACTCAACATT TGGAGAGAGC 
AGTTTCAACA ACGAGCTTCC CCAGGACAGC CAGGAAGTCA CCTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055180.1
CDS	524..1114
Translation	MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT

Target ORF information:

RefSeq Version	NM_014365.2
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014365.2

ORF Insert Sequence:

ATGGCTGACG GTCAGATGCC CTTCTCCTGC CACTACCCAA GCCGCCTGCG CCGAGACCCC 
TTCCGGGACT CTCCCCTCTC CTCTCGCCTG CTGGATGATG GCTTTGGCAT GGACCCCTTC 
CCAGACGACT TGACAGCCTC TTGGCCCGAC TGGGCTCTGC CTCGTCTCTC CTCCGCCTGG 
CCAGGCACCC TAAGGTCGGG CATGGTGCCC CGGGGCCCCA CTGCCACCGC CAGGTTTGGG 
GTGCCTGCCG AGGGCAGGAC CCCCCCACCC TTCCCTGGGG AGCCCTGGAA AGTGTGTGTG 
AATGTGCACA GCTTCAAGCC AGAGGAGTTG ATGGTGAAGA CCAAAGATGG ATACGTGGAG 
GTGTCTGGCA AACATGAAGA GAAACAGCAA GAAGGTGGCA TTGTTTCTAA GAACTTCACA 
AAGAAAATCC AGCTTCCTGC AGAGGTGGAT CCTGTGACAG TATTTGCCTC ACTTTCCCCA 
GAGGGTCTGC TGATCATCGA AGCTCCCCAG GTCCCTCCTT ACTCAACATT TGGAGAGAGC 
AGTTTCAACA ACGAGCTTCC CCAGGACAGC CAGGAAGTCA CCTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPB8 ( NM_014365.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055180.1 Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.

Price & Availability↑

CloneID	OHu26028
Clone ID Related Accession (Same CDS sequence)	NM_014365.2 , NM_014365.3
Accession Version	NM_014365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	heat shock protein beta-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002673.2. On Dec 4, 2019 this sequence version replaced NM_014365.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002673.2, AL136936.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACG GTCAGATGCC CTTCTCCTGC CACTACCCAA GCCGCCTGCG CCGAGACCCC 
TTCCGGGACT CTCCCCTCTC CTCTCGCCTG CTGGATGATG GCTTTGGCAT GGACCCCTTC 
CCAGACGACT TGACAGCCTC TTGGCCCGAC TGGGCTCTGC CTCGTCTCTC CTCCGCCTGG 
CCAGGCACCC TAAGGTCGGG CATGGTGCCC CGGGGCCCCA CTGCCACCGC CAGGTTTGGG 
GTGCCTGCCG AGGGCAGGAC CCCCCCACCC TTCCCTGGGG AGCCCTGGAA AGTGTGTGTG 
AATGTGCACA GCTTCAAGCC AGAGGAGTTG ATGGTGAAGA CCAAAGATGG ATACGTGGAG 
GTGTCTGGCA AACATGAAGA GAAACAGCAA GAAGGTGGCA TTGTTTCTAA GAACTTCACA 
AAGAAAATCC AGCTTCCTGC AGAGGTGGAT CCTGTGACAG TATTTGCCTC ACTTTCCCCA 
GAGGGTCTGC TGATCATCGA AGCTCCCCAG GTCCCTCCTT ACTCAACATT TGGAGAGAGC 
AGTTTCAACA ACGAGCTTCC CCAGGACAGC CAGGAAGTCA CCTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055180.1
CDS	383..973
Translation	MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT

Target ORF information:

RefSeq Version	NM_014365.3
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 8 (HSPB8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014365.3

ORF Insert Sequence:

ATGGCTGACG GTCAGATGCC CTTCTCCTGC CACTACCCAA GCCGCCTGCG CCGAGACCCC 
TTCCGGGACT CTCCCCTCTC CTCTCGCCTG CTGGATGATG GCTTTGGCAT GGACCCCTTC 
CCAGACGACT TGACAGCCTC TTGGCCCGAC TGGGCTCTGC CTCGTCTCTC CTCCGCCTGG 
CCAGGCACCC TAAGGTCGGG CATGGTGCCC CGGGGCCCCA CTGCCACCGC CAGGTTTGGG 
GTGCCTGCCG AGGGCAGGAC CCCCCCACCC TTCCCTGGGG AGCCCTGGAA AGTGTGTGTG 
AATGTGCACA GCTTCAAGCC AGAGGAGTTG ATGGTGAAGA CCAAAGATGG ATACGTGGAG 
GTGTCTGGCA AACATGAAGA GAAACAGCAA GAAGGTGGCA TTGTTTCTAA GAACTTCACA 
AAGAAAATCC AGCTTCCTGC AGAGGTGGAT CCTGTGACAG TATTTGCCTC ACTTTCCCCA 
GAGGGTCTGC TGATCATCGA AGCTCCCCAG GTCCCTCCTT ACTCAACATT TGGAGAGAGC 
AGTTTCAACA ACGAGCTTCC CCAGGACAGC CAGGAAGTCA CCTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered TDP-43-dependent splicing in HSPB8-related distal hereditary motor neuropathy and myofibrillar myopathy.
European journal of neurology25(1)154-163(2018 Jan)
Cortese A,Laur? M,Casali C,Nishino I,Hayashi YK,Magri S,Taroni F,Stuani C,Saveri P,Moggio M,Ripolone M,Prelle A,Pisciotta C,Sagnelli A,Pichiecchio A,Reilly MM,Buratti E,Pareyson D

Heat shock proteins HSPB8 and DNAJC5B have HCV antiviral activity.
PloS one12(11)e0188467(2017)
Braga ACS,Carneiro BM,Batista MN,Akinaga MM,Bittar C,Rahal P

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HSPB8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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