GATM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GATM
Entrez Gene ID	2628
Full Name	glycine amidinotransferase
Synonyms	AGAT,AT,CCDS3
General protein information	Preferred Names glycine amidinotransferase Names glycine amidinotransferase, mitochondrial glycine amidinotransferase (L-arginine:glycine amidinotransferase) testicular secretory protein Li 19 transamidinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.1
Summary	This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008].
Disorder MIM:	602360
Disorder Html:	Cerebral creatine deficiency syndrome 3, 612718 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001482.3	NP_001473.1	glycine amidinotransferase, mitochondrial isoform 1 precursor
NM_001321015.1	NP_001307944.1	glycine amidinotransferase, mitochondrial isoform 2
NM_001482.2	NP_001473.1	glycine amidinotransferase, mitochondrial isoform 1 precursor
NM_001321015.2	NP_001307944.1	glycine amidinotransferase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GATM	XP_001163621.2
Rattus norvegicus (Norway rat)	Gatm	NP_112293.1
	GATM	XP_001111604.1
Danio rerio (zebrafish)	gatm	NP_955825.1
Xenopus tropicalis (tropical clawed frog)	gatm	NP_988971.1
Neurospora crassa	NCU07176	XP_957401.1
Homo sapiens (human)	GATM	NP_001473.1
Bos taurus (cattle)	GATM	NP_001039343.1
Mus musculus (house mouse)	Gatm	NP_080237.1
Canis lupus familiaris (dog)	GATM	XP_544663.2
Gallus gallus (chicken)	GATM	NP_990076.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Depletion of Adipocyte Creatine Metabolism Inhibits Diet-Induced Thermogenesis and Drives Obesity.
Kazak L, Chouchani ET, Lu GZ, Jedrychowski MP, Bare CJ, Mina AI, Kumari M, Zhang S, Vuckovic I, Laznik-Bogoslavski D, Dzeja P, Banks AS, Rosen ED, Spiegelman BM
Cell metabolism26(4)660-671.e3(2017 Oct)

Laboratory diagnosis of creatine deficiency syndromes: a technical standard and guideline of the American College of Medical Genetics and Genomics.
Sharer JD, Bodamer O, Longo N, Tortorelli S, Wamelink MM, Young S
Genetics in medicine : official journal of the American College of Medical Genetics19(2)256-263(2017 Feb)

Arginine-Glycine Amidinotransferase Deficiency and Functional Characterization of Missense Variants in GATM.
DesRoches CL, Bruun T, Wang P, Marshall CR, Mercimek-Mahmutoglu S
Human mutation37(9)926-32(2016 09)

Creatine biosynthesis and transport in health and disease.
Joncquel-Chevalier Curt M, Voicu PM, Fontaine M, Dessein AF, Porchet N, Mention-Mulliez K, Dobbelaere D, Soto-Ares G, Cheillan D, Vamecq J
Biochimie119146-65(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Measurements of creatine and guanidinoacetate in plasma are recommended for the diagnosis of AGAT and GAMT deficiency.Definitive confirmation of the diagnosis requires DNA sequencing of the appropriate gene and (if molecular analysis is ambiguous) measurement of AGAT or GAMT enzyme activity or of CRTR-mediated transport .
Title: Laboratory diagnosis of creatine deficiency syndromes: a technical standard and guideline of the American College of Medical Genetics and Genomics.

Results show the functional characterization of rare missense variants in GATM which cause GATM deficiency. Seven of them report 0% of wild-type GATM activity indicating a putative pathogenicity.
Title: Arginine-Glycine Amidinotransferase Deficiency and Functional Characterization of Missense Variants in GATM.

Data suggest that creatine is provided equally by diet and by endogenous synthesis from arginine and glycine with successive involvement of arginine glycine amidinotransferase [AGAT] and guanidinoacetate methyl transferase [GAMT]. [REVIEW]
Title: Creatine biosynthesis and transport in health and disease.

AGAT deficiency is a treatable intellectual disability.
Title: Arginine:glycine amidinotransferase (AGAT) deficiency: Clinical features and long term outcomes in 16 patients diagnosed worldwide.

Meta-analysis yielded a marginal, but null, association of GATM rs9806699 with statin-induced myopathy.
Title: GATM polymorphism associated with the risk for statin-induced myopathy does not replicate in case-control analysis of 715 dyslipidemic individuals.

The following GATM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GATM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25869	NM_001482.3 Latest version!	Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu100116	NM_001321015.2 Latest version!	Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu25869	NM_001482.2	Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu100116	NM_001321015.1	Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GATM ( NM_001482.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001473.1 Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25869
Clone ID Related Accession (Same CDS sequence)	NM_001482.3 , NM_001482.2
Accession Version	NM_001482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	glycine amidinotransferase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB457418.1, BC004141.2 and CD365153.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_001482.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005559.1, SRR3476690.175565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000396659.8/ ENSP00000379895.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGGG TGCGGTGTCT GCGCGGCGGG AGCCGCGGCG CCGAGGCGGT GCACTACATC 
GGATCTCGGC TTGGACGAAC CTTGACAGGA TGGGTGCAGC GAACTTTCCA GAGCACCCAG 
GCAGCTACGG CTTCCTCCCG GAACTCCTGT GCAGCTGACG ACAAAGCCAC TGAGCCTCTG 
CCCAAGGACT GCCCTGTCTC TTCTTACAAC GAATGGGACC CCTTAGAGGA AGTGATAGTG 
GGCAGAGCAG AAAACGCCTG TGTTCCACCG TTCACCATCG AGGTGAAGGC CAACACATAT 
GAAAAGTACT GGCCATTTTA CCAGAAGCAA GGAGGGCATT ATTTTCCCAA AGATCATTTG 
AAAAAGGCTG TTGCTGAAAT TGAAGAAATG TGCAATATTT TAAAAACGGA AGGAGTGACA 
GTAAGGAGGC CTGACCCCAT TGACTGGTCA TTGAAGTATA AAACTCCTGA TTTTGAGTCT 
ACGGGTTTAT ACAGTGCAAT GCCTCGAGAC ATCCTGATAG TTGTGGGCAA TGAGATTATC 
GAGGCTCCCA TGGCATGGCG TTCACGCTTC TTTGAGTACC GAGCGTACAG GTCAATTATC 
AAAGACTACT TCCACCGTGG CGCCAAGTGG ACAACAGCTC CTAAGCCCAC AATGGCTGAT 
GAGCTTTATA ACCAGGATTA TCCCATCCAC TCTGTAGAAG ACAGACACAA ATTGGCTGCT 
CAGGGAAAAT TTGTGACAAC TGAGTTTGAG CCATGCTTTG ATGCTGCTGA CTTCATTCGA 
GCTGGAAGAG ATATTTTTGC ACAGAGAAGC CAGGTTACAA ACTACCTAGG CATTGAATGG 
ATGCGTAGGC ATCTTGCTCC AGACTACAGA GTGCATATCA TCTCCTTTAA AGATCCCAAT 
CCCATGCATA TTGATGCTAC CTTCAACATC ATTGGACCTG GTATTGTGCT TTCCAACCCT 
GACCGACCAT GTCACCAGAT TGATCTTTTC AAGAAAGCAG GATGGACTAT CATTACTCCT 
CCAACACCAA TCATCCCAGA CGATCATCCA CTCTGGATGT CATCCAAATG GCTTTCCATG 
AATGTCTTAA TGCTAGATGA AAAACGTGTT ATGGTGGATG CCAATGAAGT TCCAATTCAA 
AAGATGTTTG AAAAGCTGGG TATCACTACC ATTAAAGTTA ACATTCGTAA TGCCAATTCC 
CTGGGAGGAG GCTTCCATTG CTGGACCTGC GATGTCCGGC GCCGAGGCAC CTTACAGTCC 
TACTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001473.1
CDS	92..1363
Translation	MLRVRCLRGGSRGAEAVHYIGSRLGRTLTGWVQRTFQSTQAATASSRNSCAADDKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQSYLD

Target ORF information:

RefSeq Version	NM_001482.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001482.3

ORF Insert Sequence:

ATGCTGCGGG TGCGGTGTCT GCGCGGCGGG AGCCGCGGCG CCGAGGCGGT GCACTACATC 
GGATCTCGGC TTGGACGAAC CTTGACAGGA TGGGTGCAGC GAACTTTCCA GAGCACCCAG 
GCAGCTACGG CTTCCTCCCG GAACTCCTGT GCAGCTGACG ACAAAGCCAC TGAGCCTCTG 
CCCAAGGACT GCCCTGTCTC TTCTTACAAC GAATGGGACC CCTTAGAGGA AGTGATAGTG 
GGCAGAGCAG AAAACGCCTG TGTTCCACCG TTCACCATCG AGGTGAAGGC CAACACATAT 
GAAAAGTACT GGCCATTTTA CCAGAAGCAA GGAGGGCATT ATTTTCCCAA AGATCATTTG 
AAAAAGGCTG TTGCTGAAAT TGAAGAAATG TGCAATATTT TAAAAACGGA AGGAGTGACA 
GTAAGGAGGC CTGACCCCAT TGACTGGTCA TTGAAGTATA AAACTCCTGA TTTTGAGTCT 
ACGGGTTTAT ACAGTGCAAT GCCTCGAGAC ATCCTGATAG TTGTGGGCAA TGAGATTATC 
GAGGCTCCCA TGGCATGGCG TTCACGCTTC TTTGAGTACC GAGCGTACAG GTCAATTATC 
AAAGACTACT TCCACCGTGG CGCCAAGTGG ACAACAGCTC CTAAGCCCAC AATGGCTGAT 
GAGCTTTATA ACCAGGATTA TCCCATCCAC TCTGTAGAAG ACAGACACAA ATTGGCTGCT 
CAGGGAAAAT TTGTGACAAC TGAGTTTGAG CCATGCTTTG ATGCTGCTGA CTTCATTCGA 
GCTGGAAGAG ATATTTTTGC ACAGAGAAGC CAGGTTACAA ACTACCTAGG CATTGAATGG 
ATGCGTAGGC ATCTTGCTCC AGACTACAGA GTGCATATCA TCTCCTTTAA AGATCCCAAT 
CCCATGCATA TTGATGCTAC CTTCAACATC ATTGGACCTG GTATTGTGCT TTCCAACCCT 
GACCGACCAT GTCACCAGAT TGATCTTTTC AAGAAAGCAG GATGGACTAT CATTACTCCT 
CCAACACCAA TCATCCCAGA CGATCATCCA CTCTGGATGT CATCCAAATG GCTTTCCATG 
AATGTCTTAA TGCTAGATGA AAAACGTGTT ATGGTGGATG CCAATGAAGT TCCAATTCAA 
AAGATGTTTG AAAAGCTGGG TATCACTACC ATTAAAGTTA ACATTCGTAA TGCCAATTCC 
CTGGGAGGAG GCTTCCATTG CTGGACCTGC GATGTCCGGC GCCGAGGCAC CTTACAGTCC 
TACTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GATM ( NM_001482.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001473.1 Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25869
Clone ID Related Accession (Same CDS sequence)	NM_001482.3 , NM_001482.2
Accession Version	NM_001482.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	glycine amidinotransferase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025580.8, DB457418.1, BC004141.2 and CD365153.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced NM_001482.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005559.1, SRR1660805.78846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGGG TGCGGTGTCT GCGCGGCGGG AGCCGCGGCG CCGAGGCGGT GCACTACATC 
GGATCTCGGC TTGGACGAAC CTTGACAGGA TGGGTGCAGC GAACTTTCCA GAGCACCCAG 
GCAGCTACGG CTTCCTCCCG GAACTCCTGT GCAGCTGACG ACAAAGCCAC TGAGCCTCTG 
CCCAAGGACT GCCCTGTCTC TTCTTACAAC GAATGGGACC CCTTAGAGGA AGTGATAGTG 
GGCAGAGCAG AAAACGCCTG TGTTCCACCG TTCACCATCG AGGTGAAGGC CAACACATAT 
GAAAAGTACT GGCCATTTTA CCAGAAGCAA GGAGGGCATT ATTTTCCCAA AGATCATTTG 
AAAAAGGCTG TTGCTGAAAT TGAAGAAATG TGCAATATTT TAAAAACGGA AGGAGTGACA 
GTAAGGAGGC CTGACCCCAT TGACTGGTCA TTGAAGTATA AAACTCCTGA TTTTGAGTCT 
ACGGGTTTAT ACAGTGCAAT GCCTCGAGAC ATCCTGATAG TTGTGGGCAA TGAGATTATC 
GAGGCTCCCA TGGCATGGCG TTCACGCTTC TTTGAGTACC GAGCGTACAG GTCAATTATC 
AAAGACTACT TCCACCGTGG CGCCAAGTGG ACAACAGCTC CTAAGCCCAC AATGGCTGAT 
GAGCTTTATA ACCAGGATTA TCCCATCCAC TCTGTAGAAG ACAGACACAA ATTGGCTGCT 
CAGGGAAAAT TTGTGACAAC TGAGTTTGAG CCATGCTTTG ATGCTGCTGA CTTCATTCGA 
GCTGGAAGAG ATATTTTTGC ACAGAGAAGC CAGGTTACAA ACTACCTAGG CATTGAATGG 
ATGCGTAGGC ATCTTGCTCC AGACTACAGA GTGCATATCA TCTCCTTTAA AGATCCCAAT 
CCCATGCATA TTGATGCTAC CTTCAACATC ATTGGACCTG GTATTGTGCT TTCCAACCCT 
GACCGACCAT GTCACCAGAT TGATCTTTTC AAGAAAGCAG GATGGACTAT CATTACTCCT 
CCAACACCAA TCATCCCAGA CGATCATCCA CTCTGGATGT CATCCAAATG GCTTTCCATG 
AATGTCTTAA TGCTAGATGA AAAACGTGTT ATGGTGGATG CCAATGAAGT TCCAATTCAA 
AAGATGTTTG AAAAGCTGGG TATCACTACC ATTAAAGTTA ACATTCGTAA TGCCAATTCC 
CTGGGAGGAG GCTTCCATTG CTGGACCTGC GATGTCCGGC GCCGAGGCAC CTTACAGTCC 
TACTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001473.1
CDS	330..1601
Translation	MLRVRCLRGGSRGAEAVHYIGSRLGRTLTGWVQRTFQSTQAATASSRNSCAADDKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQSYLD

Target ORF information:

RefSeq Version	NM_001482.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine amidinotransferase (GATM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001482.2

ORF Insert Sequence:

ATGCTGCGGG TGCGGTGTCT GCGCGGCGGG AGCCGCGGCG CCGAGGCGGT GCACTACATC 
GGATCTCGGC TTGGACGAAC CTTGACAGGA TGGGTGCAGC GAACTTTCCA GAGCACCCAG 
GCAGCTACGG CTTCCTCCCG GAACTCCTGT GCAGCTGACG ACAAAGCCAC TGAGCCTCTG 
CCCAAGGACT GCCCTGTCTC TTCTTACAAC GAATGGGACC CCTTAGAGGA AGTGATAGTG 
GGCAGAGCAG AAAACGCCTG TGTTCCACCG TTCACCATCG AGGTGAAGGC CAACACATAT 
GAAAAGTACT GGCCATTTTA CCAGAAGCAA GGAGGGCATT ATTTTCCCAA AGATCATTTG 
AAAAAGGCTG TTGCTGAAAT TGAAGAAATG TGCAATATTT TAAAAACGGA AGGAGTGACA 
GTAAGGAGGC CTGACCCCAT TGACTGGTCA TTGAAGTATA AAACTCCTGA TTTTGAGTCT 
ACGGGTTTAT ACAGTGCAAT GCCTCGAGAC ATCCTGATAG TTGTGGGCAA TGAGATTATC 
GAGGCTCCCA TGGCATGGCG TTCACGCTTC TTTGAGTACC GAGCGTACAG GTCAATTATC 
AAAGACTACT TCCACCGTGG CGCCAAGTGG ACAACAGCTC CTAAGCCCAC AATGGCTGAT 
GAGCTTTATA ACCAGGATTA TCCCATCCAC TCTGTAGAAG ACAGACACAA ATTGGCTGCT 
CAGGGAAAAT TTGTGACAAC TGAGTTTGAG CCATGCTTTG ATGCTGCTGA CTTCATTCGA 
GCTGGAAGAG ATATTTTTGC ACAGAGAAGC CAGGTTACAA ACTACCTAGG CATTGAATGG 
ATGCGTAGGC ATCTTGCTCC AGACTACAGA GTGCATATCA TCTCCTTTAA AGATCCCAAT 
CCCATGCATA TTGATGCTAC CTTCAACATC ATTGGACCTG GTATTGTGCT TTCCAACCCT 
GACCGACCAT GTCACCAGAT TGATCTTTTC AAGAAAGCAG GATGGACTAT CATTACTCCT 
CCAACACCAA TCATCCCAGA CGATCATCCA CTCTGGATGT CATCCAAATG GCTTTCCATG 
AATGTCTTAA TGCTAGATGA AAAACGTGTT ATGGTGGATG CCAATGAAGT TCCAATTCAA 
AAGATGTTTG AAAAGCTGGG TATCACTACC ATTAAAGTTA ACATTCGTAA TGCCAATTCC 
CTGGGAGGAG GCTTCCATTG CTGGACCTGC GATGTCCGGC GCCGAGGCAC CTTACAGTCC 
TACTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GATM ( NM_001321015.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307944.1 Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100116
Clone ID Related Accession (Same CDS sequence)	NM_001321015.1 , NM_001321015.2
Accession Version	NM_001321015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	glycine amidinotransferase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC039389.1, AC025580.8 and AI253042.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.96006.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGCAATA TTTTAAAAAC GGAAGGAGTG ACAGTAAGGA GGCCTGACCC CATTGACTGG 
TCATTGAAGT ATAAAACTCC TGATTTTGAG TCTACGGGTT TATACAGTGC AATGCCTCGA 
GACATCCTGA TAGTTGTGGG CAATGAGATT ATCGAGGCTC CCATGGCATG GCGTTCACGC 
TTCTTTGAGT ACCGAGCGTA CAGGTCAATT ATCAAAGACT ACTTCCACCG TGGCGCCAAG 
TGGACAACAG CTCCTAAGCC CACAATGGCT GATGAGCTTT ATAACCAGGA TTATCCCATC 
CACTCTGTAG AAGACAGACA CAAATTGGCT GCTCAGGGAA AATTTGTGAC AACTGAGTTT 
GAGCCATGCT TTGATGCTGC TGACTTCATT CGAGCTGGAA GAGATATTTT TGCACAGAGA 
AGCCAGGTTA CAAACTACCT AGGCATTGAA TGGATGCGTA GGCATCTTGC TCCAGACTAC 
AGAGTGCATA TCATCTCCTT TAAAGATCCC AATCCCATGC ATATTGATGC TACCTTCAAC 
ATCATTGGAC CTGGTATTGT GCTTTCCAAC CCTGACCGAC CATGTCACCA GATTGATCTT 
TTCAAGAAAG CAGGATGGAC TATCATTACT CCTCCAACAC CAATCATCCC AGACGATCAT 
CCACTCTGGA TGTCATCCAA ATGGCTTTCC ATGAATGTCT TAATGCTAGA TGAAAAACGT 
GTTATGGTGG ATGCCAATGA AGTTCCAATT CAAAAGATGT TTGAAAAGCT GGGTATCACT 
ACCATTAAAG TTAACATTCG TAATGCCAAT TCCCTGGGAG GAGGCTTCCA TTGCTGGACC 
TGCGATGTCC GGCGCCGAGG CACCTTACAG TCCTACTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307944.1
CDS	1025..1909
Translation	MCNILKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQSYLD

Target ORF information:

RefSeq Version	NM_001321015.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321015.1

ORF Insert Sequence:

ATGTGCAATA TTTTAAAAAC GGAAGGAGTG ACAGTAAGGA GGCCTGACCC CATTGACTGG 
TCATTGAAGT ATAAAACTCC TGATTTTGAG TCTACGGGTT TATACAGTGC AATGCCTCGA 
GACATCCTGA TAGTTGTGGG CAATGAGATT ATCGAGGCTC CCATGGCATG GCGTTCACGC 
TTCTTTGAGT ACCGAGCGTA CAGGTCAATT ATCAAAGACT ACTTCCACCG TGGCGCCAAG 
TGGACAACAG CTCCTAAGCC CACAATGGCT GATGAGCTTT ATAACCAGGA TTATCCCATC 
CACTCTGTAG AAGACAGACA CAAATTGGCT GCTCAGGGAA AATTTGTGAC AACTGAGTTT 
GAGCCATGCT TTGATGCTGC TGACTTCATT CGAGCTGGAA GAGATATTTT TGCACAGAGA 
AGCCAGGTTA CAAACTACCT AGGCATTGAA TGGATGCGTA GGCATCTTGC TCCAGACTAC 
AGAGTGCATA TCATCTCCTT TAAAGATCCC AATCCCATGC ATATTGATGC TACCTTCAAC 
ATCATTGGAC CTGGTATTGT GCTTTCCAAC CCTGACCGAC CATGTCACCA GATTGATCTT 
TTCAAGAAAG CAGGATGGAC TATCATTACT CCTCCAACAC CAATCATCCC AGACGATCAT 
CCACTCTGGA TGTCATCCAA ATGGCTTTCC ATGAATGTCT TAATGCTAGA TGAAAAACGT 
GTTATGGTGG ATGCCAATGA AGTTCCAATT CAAAAGATGT TTGAAAAGCT GGGTATCACT 
ACCATTAAAG TTAACATTCG TAATGCCAAT TCCCTGGGAG GAGGCTTCCA TTGCTGGACC 
TGCGATGTCC GGCGCCGAGG CACCTTACAG TCCTACTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GATM ( NM_001321015.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307944.1 Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.

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CloneID	OHu100116
Clone ID Related Accession (Same CDS sequence)	NM_001321015.1 , NM_001321015.2
Accession Version	NM_001321015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	glycine amidinotransferase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC039389.1, AC025580.8 and AI253042.1. On May 31, 2019 this sequence version replaced NM_001321015.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.96006.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGCAATA TTTTAAAAAC GGAAGGAGTG ACAGTAAGGA GGCCTGACCC CATTGACTGG 
TCATTGAAGT ATAAAACTCC TGATTTTGAG TCTACGGGTT TATACAGTGC AATGCCTCGA 
GACATCCTGA TAGTTGTGGG CAATGAGATT ATCGAGGCTC CCATGGCATG GCGTTCACGC 
TTCTTTGAGT ACCGAGCGTA CAGGTCAATT ATCAAAGACT ACTTCCACCG TGGCGCCAAG 
TGGACAACAG CTCCTAAGCC CACAATGGCT GATGAGCTTT ATAACCAGGA TTATCCCATC 
CACTCTGTAG AAGACAGACA CAAATTGGCT GCTCAGGGAA AATTTGTGAC AACTGAGTTT 
GAGCCATGCT TTGATGCTGC TGACTTCATT CGAGCTGGAA GAGATATTTT TGCACAGAGA 
AGCCAGGTTA CAAACTACCT AGGCATTGAA TGGATGCGTA GGCATCTTGC TCCAGACTAC 
AGAGTGCATA TCATCTCCTT TAAAGATCCC AATCCCATGC ATATTGATGC TACCTTCAAC 
ATCATTGGAC CTGGTATTGT GCTTTCCAAC CCTGACCGAC CATGTCACCA GATTGATCTT 
TTCAAGAAAG CAGGATGGAC TATCATTACT CCTCCAACAC CAATCATCCC AGACGATCAT 
CCACTCTGGA TGTCATCCAA ATGGCTTTCC ATGAATGTCT TAATGCTAGA TGAAAAACGT 
GTTATGGTGG ATGCCAATGA AGTTCCAATT CAAAAGATGT TTGAAAAGCT GGGTATCACT 
ACCATTAAAG TTAACATTCG TAATGCCAAT TCCCTGGGAG GAGGCTTCCA TTGCTGGACC 
TGCGATGTCC GGCGCCGAGG CACCTTACAG TCCTACTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307944.1
CDS	935..1819
Translation	MCNILKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQSYLD

Target ORF information:

RefSeq Version	NM_001321015.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycine amidinotransferase (GATM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321015.2

ORF Insert Sequence:

ATGTGCAATA TTTTAAAAAC GGAAGGAGTG ACAGTAAGGA GGCCTGACCC CATTGACTGG 
TCATTGAAGT ATAAAACTCC TGATTTTGAG TCTACGGGTT TATACAGTGC AATGCCTCGA 
GACATCCTGA TAGTTGTGGG CAATGAGATT ATCGAGGCTC CCATGGCATG GCGTTCACGC 
TTCTTTGAGT ACCGAGCGTA CAGGTCAATT ATCAAAGACT ACTTCCACCG TGGCGCCAAG 
TGGACAACAG CTCCTAAGCC CACAATGGCT GATGAGCTTT ATAACCAGGA TTATCCCATC 
CACTCTGTAG AAGACAGACA CAAATTGGCT GCTCAGGGAA AATTTGTGAC AACTGAGTTT 
GAGCCATGCT TTGATGCTGC TGACTTCATT CGAGCTGGAA GAGATATTTT TGCACAGAGA 
AGCCAGGTTA CAAACTACCT AGGCATTGAA TGGATGCGTA GGCATCTTGC TCCAGACTAC 
AGAGTGCATA TCATCTCCTT TAAAGATCCC AATCCCATGC ATATTGATGC TACCTTCAAC 
ATCATTGGAC CTGGTATTGT GCTTTCCAAC CCTGACCGAC CATGTCACCA GATTGATCTT 
TTCAAGAAAG CAGGATGGAC TATCATTACT CCTCCAACAC CAATCATCCC AGACGATCAT 
CCACTCTGGA TGTCATCCAA ATGGCTTTCC ATGAATGTCT TAATGCTAGA TGAAAAACGT 
GTTATGGTGG ATGCCAATGA AGTTCCAATT CAAAAGATGT TTGAAAAGCT GGGTATCACT 
ACCATTAAAG TTAACATTCG TAATGCCAAT TCCCTGGGAG GAGGCTTCCA TTGCTGGACC 
TGCGATGTCC GGCGCCGAGG CACCTTACAG TCCTACTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Depletion of Adipocyte Creatine Metabolism Inhibits Diet-Induced Thermogenesis and Drives Obesity.
Cell metabolism26(4)660-671.e3(2017 Oct)
Kazak L,Chouchani ET,Lu GZ,Jedrychowski MP,Bare CJ,Mina AI,Kumari M,Zhang S,Vuckovic I,Laznik-Bogoslavski D,Dzeja P,Banks AS,Rosen ED,Spiegelman BM

Laboratory diagnosis of creatine deficiency syndromes: a technical standard and guideline of the American College of Medical Genetics and Genomics.
Genetics in medicine : official journal of the American College of Medical Genetics19(2)256-263(2017 Feb)
Sharer JD,Bodamer O,Longo N,Tortorelli S,Wamelink MM,Young S

Arginine-Glycine Amidinotransferase Deficiency and Functional Characterization of Missense Variants in GATM.
Human mutation37(9)926-32(2016 09)
DesRoches CL,Bruun T,Wang P,Marshall CR,Mercimek-Mahmutoglu S

Creatine biosynthesis and transport in health and disease.
Biochimie119146-65(2015 Dec)
Joncquel-Chevalier Curt M,Voicu PM,Fontaine M,Dessein AF,Porchet N,Mention-Mulliez K,Dobbelaere D,Soto-Ares G,Cheillan D,Vamecq J

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GATM cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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