HERC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC4
Entrez Gene ID	26091
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 4
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase 4 Names probable E3 ubiquitin-protein ligase HERC4 HECT domain and RCC1-like domain-containing protein 4 HECT-type E3 ubiquitin transferase HERC4 hect domain and RLD 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q21.3
Summary	HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609248

mRNA and Protein(s)

mRNA	Protein	Name
XM_017016041.1	XP_016871530.1	probable E3 ubiquitin-protein ligase HERC4 isoform X6
XM_024447932.1	XP_024303700.1	probable E3 ubiquitin-protein ligase HERC4 isoform X9
XM_024447927.1	XP_024303695.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_011539597.3	XP_011537899.1	probable E3 ubiquitin-protein ligase HERC4 isoform X7
XM_011539592.2	XP_011537894.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_024447928.1	XP_024303696.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
NM_015601.3	NP_056416.2	probable E3 ubiquitin-protein ligase HERC4 isoform b
NM_001278187.1	NP_001265116.1	probable E3 ubiquitin-protein ligase HERC4 isoform e
XM_011539592.3	XP_011537894.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_024447929.1	XP_024303697.1	probable E3 ubiquitin-protein ligase HERC4 isoform X3
XM_024447930.1	XP_024303698.1	probable E3 ubiquitin-protein ligase HERC4 isoform X3
XM_024447931.1	XP_024303699.1	probable E3 ubiquitin-protein ligase HERC4 isoform X4
NM_001278186.2	NP_001265115.1	probable E3 ubiquitin-protein ligase HERC4 isoform d
XM_024447933.1	XP_024303701.1	probable E3 ubiquitin-protein ligase HERC4 isoform X10
NM_001278186.1	NP_001265115.1	probable E3 ubiquitin-protein ligase HERC4 isoform d
NM_001278185.2	NP_001265114.1	probable E3 ubiquitin-protein ligase HERC4 isoform c
NM_001278187.2	NP_001265116.1	probable E3 ubiquitin-protein ligase HERC4 isoform e
XM_011539593.3	XP_011537895.1	probable E3 ubiquitin-protein ligase HERC4 isoform X2
XM_011539594.2	XP_011537896.1	probable E3 ubiquitin-protein ligase HERC4 isoform X5
XM_011539595.2	XP_011537897.1	probable E3 ubiquitin-protein ligase HERC4 isoform X5
XM_011539596.2	XP_011537898.1	probable E3 ubiquitin-protein ligase HERC4 isoform X6
NM_001278185.1	NP_001265114.1	probable E3 ubiquitin-protein ligase HERC4 isoform c
XM_011539593.2	XP_011537895.1	probable E3 ubiquitin-protein ligase HERC4 isoform X2
XM_011539597.2	XP_011537899.1	probable E3 ubiquitin-protein ligase HERC4 isoform X5
NM_022079.2	NP_071362.1	probable E3 ubiquitin-protein ligase HERC4 isoform a
NM_022079.3	NP_071362.1	probable E3 ubiquitin-protein ligase HERC4 isoform a
XM_017016041.2	XP_016871530.1	probable E3 ubiquitin-protein ligase HERC4 isoform X8
NM_015601.4	NP_056416.2	probable E3 ubiquitin-protein ligase HERC4 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	HERC4	NP_071362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006994	XP_308776.4
Canis lupus familiaris (dog)	HERC4	XP_005618991.1
Danio rerio (zebrafish)	herc4	XP_685685.5
Drosophila melanogaster (fruit fly)	CG9153	NP_001261249.1
Bos taurus (cattle)	HERC4	NP_001070362.2
Pan troglodytes (chimpanzee)	HERC4	XP_001167753.1
Mus musculus (house mouse)	Herc4	NP_084390.1
Rattus norvegicus (Norway rat)	Herc4	NP_001012074.1
Gallus gallus (chicken)	HERC4	XP_004942077.1
Arabidopsis thaliana (thale cress)	UVR8	NP_201191.1
Oryza sativa (rice)	Os02g0554100	NP_001047115.1
Xenopus tropicalis (tropical clawed frog)	herc4	NP_001122122.1
	HERC4	XP_001087608.1
Oryza sativa (rice)	Os04g0435700	NP_001052849.1

Gene Ontology
Bibliography

Related articles in PubMed

SIRT1/HERC4 Locus Associated With Bisphosphonate-Induced Osteonecrosis of the Jaw: An Exome-Wide Association Analysis.
Yang G, Hamadeh IS, Katz J, Riva A, Lakatos P, Balla B, Kosa J, Vaszilko M, Pelliccioni GA, Davis N, Langaee TY, Moreb JS, Gong Y
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research33(1)91-98(2018 Jan)

HERC4 Is Overexpressed in Hepatocellular Carcinoma and Contributes to the Proliferation and Migration of Hepatocellular Carcinoma Cells.
Zheng Y, Li J, Pan C, Zhou G, Zhuge L, Jin L, Fang P
DNA and cell biology36(6)490-500(2017 Jun)

Expression of HERC4 in lung cancer and its correlation with clinicopathological parameters.
Zeng WL, Chen YW, Zhou H, Zhou JY, Wei M, Shi R
Asian Pacific journal of cancer prevention : APJCP16(2)513-7(2015)

Disruption of the ubiquitin ligase HERC4 causes defects in spermatozoon maturation and impaired fertility.
Rodriguez CI, Stewart CL
Developmental biology312(2)501-8(2007 Dec)

A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
Grupe A, Li Y, Rowland C, Nowotny P, Hinrichs AL, Smemo S, Kauwe JS, Maxwell TJ, Cherny S, Doil L, Tacey K, van Luchene R, Myers A, Wavrant-De Vri?ze F, Kaleem M, Hollingworth P, Jehu L, Foy C, Archer N, Hamilton G, Holmans P, Morris CM, Catanese J, Sninsky J, White TJ, Powell J, Hardy J, O'Donovan M, Lovestone S, Jones L, Morris JC, Thal L, Owen M, Williams J, Goate A
American journal of human genetics78(1)78-88(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HERC4 has an effect on the occurrence and development of hepatoma carcinoma
Title: HERC4 Is Overexpressed in Hepatocellular Carcinoma and Contributes to the Proliferation and Migration of Hepatocellular Carcinoma Cells.

High HERC4 expression is associated with lung cancer.
Title: Expression of HERC4 in lung cancer and its correlation with clinicopathological parameters.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.

HERC4 ancestor emerged in nematodes and that the family expanded throughout evolution. The mRNA expression pattern of the HERC4 was found to be diverse in selected tissues and cells
Title: The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects.

The following HERC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07060	NM_015601.4 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu07060	XM_024447931.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu04334	NM_001278187.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu04334	XM_024447933.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu00493	NM_001278185.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu00507	NM_001278186.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu66795	XM_011539596.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu66796	XM_011539597.3 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu106447	XM_024447932.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu91278	XM_017016041.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu31116	NM_022079.3 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu31116	XM_024447929.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu31116	XM_024447930.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu66792	XM_024447927.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66792	XM_011539592.3 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66792	XM_024447928.1 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66793	XM_011539593.3 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66794	XM_011539595.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66794	XM_011539594.2 Latest version!	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu66792	XM_011539592.2	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu00507	NM_001278186.1	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu04334	NM_001278187.1	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu00493	NM_001278185.1	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu66793	XM_011539593.2	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu66796	XM_011539597.2	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu31116	NM_022079.2	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OHu91278	XM_017016041.1	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu07060	NM_015601.3	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC4 ( NM_015601.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056416.2 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.

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CloneID	OHu07060
Clone ID Related Accession (Same CDS sequence)	NM_015601.3 , XM_024447931.1 , NM_015601.4
Accession Version	NM_015601.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, BC039600.1 and BC018114.1. On Apr 19, 2007 this sequence version replaced NM_015601.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.147497.1, SRR1660803.84243.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056416.2
CDS	312..3461
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_015601.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015601.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_015601.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056416.2 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.

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CloneID	OHu07060
Clone ID Related Accession (Same CDS sequence)	NM_015601.3 , XM_024447931.1 , NM_015601.4
Accession Version	NM_015601.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, BC039600.1 and BC018114.1. On Jun 2, 2019 this sequence version replaced NM_015601.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.147497.1, BC039600.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056416.2
CDS	249..3398
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_015601.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015601.4

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447931.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303699.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu07060
Clone ID Related Accession (Same CDS sequence)	NM_015601.3 , XM_024447931.1 , NM_015601.4
Accession Version	XM_024447931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303699.1
CDS	616..3765
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447931.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447931.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGTACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA GACCGATGCA GTCTTACAGA TGCAGATGGC TATTGATCAG 
GCCCACAGGC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TGGTGCGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAACA TAGATTACAA GAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTG 
GATGCAGGAG GGGTGCGCAA AGAATTTTTC TTGCTCATCA TGAGGGAATT ATTGGATCCT 
AAATACGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT CCATTTGATT GGTGTTATCT GTGGCTTAGC AATTTATAAT 
TGTACCATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTACT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA AGAACTAATG CCTGATGTTG GGAGAAGCAT GCAACAGTTA 
CTGGATTATC CAGAAGATGA CATAGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAAGA GTTTGTCGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC 
CTTCTGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTCA TTGGAAATAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAAT ATTGGGCAGA ACATCCTACG 
ATAAAAATTT TTTGGGAAGT ATTTCACGAA TTACCATTGG AAAAGAAGAA ACAGTTTCTG 
TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT GAAACTAGTC 
ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACTTG TTTTAATCTT 
CTGGATCTTC CAAAATATAC AGAAAAAGAA ACTCTACGCT CTAAACTGAT CCAAGCTATT 
GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278187.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265116.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.

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CloneID	OHu04334
Clone ID Related Accession (Same CDS sequence)	NM_001278187.1 , XM_024447933.1 , NM_001278187.2
Accession Version	NM_001278187.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, AY650034.1 and BX101651.1. Transcript Variant: This variant (5) has an alternate 3'-most exon in place of most of the rest of the transcript compared to variant 1. The resulting isoform (e) has a shorter and distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK026808.1, AY650034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267781 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265116.1
CDS	312..644
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEFRSCFPGRSAMAPSRLTATSASQVQAILLPQPPE

Target ORF information:

RefSeq Version	NM_001278187.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278187.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278187.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265116.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.

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CloneID	OHu04334
Clone ID Related Accession (Same CDS sequence)	NM_001278187.1 , XM_024447933.1 , NM_001278187.2
Accession Version	NM_001278187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY650034.1, BX101651.1 and AC024258.9. On Jun 2, 2019 this sequence version replaced NM_001278187.1. Transcript Variant: This variant (5) has an alternate 3'-most exon in place of most of the rest of the transcript compared to variant 1. The resulting isoform (e) has a shorter and distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AY650034.1, AK026808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267781 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265116.1
CDS	249..581
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEFRSCFPGRSAMAPSRLTATSASQVQAILLPQPPE

Target ORF information:

RefSeq Version	NM_001278187.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278187.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447933.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303701.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X15, mRNA.

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CloneID	OHu04334
Clone ID Related Accession (Same CDS sequence)	NM_001278187.1 , XM_024447933.1 , NM_001278187.2
Accession Version	XM_024447933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303701.1
CDS	616..948
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEFRSCFPGRSAMAPSRLTATSASQVQAILLPQPPE

Target ORF information:

RefSeq Version	XM_024447933.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447933.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGTT TCGCTCTTGT 
TTCCCAGGCC GGAGTGCAAT GGCGCCATCT CGGCTCACCG CAACCTCTGC CTCCCAGGTT 
CAAGCAATTC TCCTGCCTCA GCCTCCCGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278185.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265114.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.

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CloneID	OHu00493
Clone ID Related Accession (Same CDS sequence)	NM_001278185.2 , NM_001278185.1
Accession Version	NM_001278185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY650033.1, AY650032.1 and BC018114.1. On Jun 2, 2019 this sequence version replaced NM_001278185.1. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AY650033.1, SRR1803611.123690.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GATCACAGTT GAAAACTTTG GTGCAACAGA AGTGAAAGAG 
CTGGTTCTAA ATGGTGCAGA CACAGCTGTT AACAAACAAA ATCGGCAAGA GTTTGTCGAT 
GCTTATGTGG ATTACATATT CAATAAATCA GTGGCTTCCT TATTTGATGC TTTTCATGCG 
GGCTTTCATA AGGTCTGTGG AGGAAAAGTC CTTCTGCTCT TTCAGCCTAA TGAACTACAA 
GCAATGGTCA TTGGAAATAC AAATTATGAT TGGAAGGAAC TGGAAAAGAA TACAGAATAC 
AAAGGGGAAT ATTGGGCAGA ACATCCTACG ATAAAAATTT TTTGGGAAGT ATTTCACGAA 
TTACCATTGG AAAAGAAGAA ACAGTTTCTG TTATTTTTGA CAGGTAGTGA TCGCATTCCT 
ATTCTTGGTA TGAAGAGTCT GAAACTAGTC ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT 
CTCCCAGTTT CCCATACTTG TTTTAATCTT CTGGATCTTC CAAAATATAC AGAAAAAGAA 
ACTCTACGCT CTAAACTGAT CCAAGCTATT GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265114.1
CDS	249..3188
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_001278185.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278185.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GATCACAGTT GAAAACTTTG GTGCAACAGA AGTGAAAGAG 
CTGGTTCTAA ATGGTGCAGA CACAGCTGTT AACAAACAAA ATCGGCAAGA GTTTGTCGAT 
GCTTATGTGG ATTACATATT CAATAAATCA GTGGCTTCCT TATTTGATGC TTTTCATGCG 
GGCTTTCATA AGGTCTGTGG AGGAAAAGTC CTTCTGCTCT TTCAGCCTAA TGAACTACAA 
GCAATGGTCA TTGGAAATAC AAATTATGAT TGGAAGGAAC TGGAAAAGAA TACAGAATAC 
AAAGGGGAAT ATTGGGCAGA ACATCCTACG ATAAAAATTT TTTGGGAAGT ATTTCACGAA 
TTACCATTGG AAAAGAAGAA ACAGTTTCTG TTATTTTTGA CAGGTAGTGA TCGCATTCCT 
ATTCTTGGTA TGAAGAGTCT GAAACTAGTC ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT 
CTCCCAGTTT CCCATACTTG TTTTAATCTT CTGGATCTTC CAAAATATAC AGAAAAAGAA 
ACTCTACGCT CTAAACTGAT CCAAGCTATT GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278185.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265114.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00493
Clone ID Related Accession (Same CDS sequence)	NM_001278185.2 , NM_001278185.1
Accession Version	NM_001278185.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, AY650033.1, AY650032.1 and BC018114.1. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AY650033.1, SRR1803611.123690.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GATCACAGTT GAAAACTTTG GTGCAACAGA AGTGAAAGAG 
CTGGTTCTAA ATGGTGCAGA CACAGCTGTT AACAAACAAA ATCGGCAAGA GTTTGTCGAT 
GCTTATGTGG ATTACATATT CAATAAATCA GTGGCTTCCT TATTTGATGC TTTTCATGCG 
GGCTTTCATA AGGTCTGTGG AGGAAAAGTC CTTCTGCTCT TTCAGCCTAA TGAACTACAA 
GCAATGGTCA TTGGAAATAC AAATTATGAT TGGAAGGAAC TGGAAAAGAA TACAGAATAC 
AAAGGGGAAT ATTGGGCAGA ACATCCTACG ATAAAAATTT TTTGGGAAGT ATTTCACGAA 
TTACCATTGG AAAAGAAGAA ACAGTTTCTG TTATTTTTGA CAGGTAGTGA TCGCATTCCT 
ATTCTTGGTA TGAAGAGTCT GAAACTAGTC ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT 
CTCCCAGTTT CCCATACTTG TTTTAATCTT CTGGATCTTC CAAAATATAC AGAAAAAGAA 
ACTCTACGCT CTAAACTGAT CCAAGCTATT GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265114.1
CDS	312..3251
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_001278185.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278185.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GATCACAGTT GAAAACTTTG GTGCAACAGA AGTGAAAGAG 
CTGGTTCTAA ATGGTGCAGA CACAGCTGTT AACAAACAAA ATCGGCAAGA GTTTGTCGAT 
GCTTATGTGG ATTACATATT CAATAAATCA GTGGCTTCCT TATTTGATGC TTTTCATGCG 
GGCTTTCATA AGGTCTGTGG AGGAAAAGTC CTTCTGCTCT TTCAGCCTAA TGAACTACAA 
GCAATGGTCA TTGGAAATAC AAATTATGAT TGGAAGGAAC TGGAAAAGAA TACAGAATAC 
AAAGGGGAAT ATTGGGCAGA ACATCCTACG ATAAAAATTT TTTGGGAAGT ATTTCACGAA 
TTACCATTGG AAAAGAAGAA ACAGTTTCTG TTATTTTTGA CAGGTAGTGA TCGCATTCCT 
ATTCTTGGTA TGAAGAGTCT GAAACTAGTC ATCCAGTCCA CAGGAGGTGG TGAGGAGTAT 
CTCCCAGTTT CCCATACTTG TTTTAATCTT CTGGATCTTC CAAAATATAC AGAAAAAGAA 
ACTCTACGCT CTAAACTGAT CCAAGCTATT GATCACAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278186.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265115.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu00507
Clone ID Related Accession (Same CDS sequence)	NM_001278186.2 , NM_001278186.1
Accession Version	NM_001278186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY650032.1 and BC018114.1. On Jun 2, 2019 this sequence version replaced NM_001278186.1. Transcript Variant: This variant (4) lacks two alternate exons compared to variant 1, and one causes a frameshift. These differences cause translation initiation at a downstream AUG and result in an isoform (d) with a shorter and distinct N-terminus and lacking an alternate internal segment, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY650032.1, BC015043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAAA TGGAAGGTGG AGTGTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGC 
CATAATTCTA CCAGTCATGA AATAAACCCA AGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTCACTGAGA TTGCTTGTGG ACGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTACTCTT TTGGGCTTGG TGGTAATGGG CAGCTGGGAA CCGGTTCAAC AAGCAACAGG 
AAAAGCCCCT TTACTGTAAA AGGAAATTGG TACCCCTATA ATGGGCAGTG TCTACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTAAAA AGAATTTTCT CAGGGGGAGA TCAAAGCTTT 
TCACATTACT CTAGTCCCCA GAACTGTGGG CCACCAGATG ACTTCAGATG TCCCAATCCG 
ACAAAGCAGA TCTGGACAGT GAATGAAGCT CTAATTCAGA AATGGCTGAG CTATCCTTCT 
GGAAGGTTTC CTGTGGAGAT AGCCAATGAG ATAGATGGAA CGTTTTCTTC CTCTGGTTGC 
CTAAATGGAA GTTTTTTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG TACCAGATTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCGCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGCTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGTAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAGACCG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ATCAGGCCCA CAGGCAGAAT GTCTCCTCTC TTTTTCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTGGTG CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAACATAGAT TACAAGAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTGGATGC AGGAGGGGTG CGCAAAGAAT TTTTCTTGCT CATCATGAGG 
GAATTATTGG ATCCTAAATA CGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTCCATT TGATTGGTGT TATCTGTGGC 
TTAGCAATTT ATAATTGTAC CATTGTGGAC CTCCATTTTC CTTTGGCTTT ATATAAGAAA 
CTACTGAAAA AGAAGCCATC CTTGGATGAT TTGAAAGAAC TAATGCCTGA TGTTGGGAGA 
AGCATGCAAC AGTTACTGGA TTATCCAGAA GATGACATAG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAAGAGTTTG TCGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTATTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AAGTCCTTCT GCTCTTTCAG CCTAATGAAC TACAAGCAAT GGTCATTGGA 
AATACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAATATTGG 
GCAGAACATC CTACGATAAA AATTTTTTGG GAAGTATTTC ACGAATTACC ATTGGAAAAG 
AAGAAACAGT TTCTGTTATT TTTGACAGGT AGTGATCGCA TTCCTATTCT TGGTATGAAG 
AGTCTGAAAC TAGTCATCCA GTCCACAGGA GGTGGTGAGG AGTATCTCCC AGTTTCCCAT 
ACTTGTTTTA ATCTTCTGGA TCTTCCAAAA TATACAGAAA AAGAAACTCT ACGCTCTAAA 
CTGATCCAAG CTATTGATCA CAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265115.1
CDS	762..3146
Translation	MMKMEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_001278186.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278186.2

ORF Insert Sequence:

ATGATGAAAA TGGAAGGTGG AGTGTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGC 
CATAATTCTA CCAGTCATGA AATAAACCCA AGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTCACTGAGA TTGCTTGTGG ACGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTACTCTT TTGGGCTTGG TGGTAATGGG CAGCTGGGAA CCGGTTCAAC AAGCAACAGG 
AAAAGCCCCT TTACTGTAAA AGGAAATTGG TACCCCTATA ATGGGCAGTG TCTACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTAAAA AGAATTTTCT CAGGGGGAGA TCAAAGCTTT 
TCACATTACT CTAGTCCCCA GAACTGTGGG CCACCAGATG ACTTCAGATG TCCCAATCCG 
ACAAAGCAGA TCTGGACAGT GAATGAAGCT CTAATTCAGA AATGGCTGAG CTATCCTTCT 
GGAAGGTTTC CTGTGGAGAT AGCCAATGAG ATAGATGGAA CGTTTTCTTC CTCTGGTTGC 
CTAAATGGAA GTTTTTTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG TACCAGATTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCGCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGCTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGTAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAGACCG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ATCAGGCCCA CAGGCAGAAT GTCTCCTCTC TTTTTCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTGGTG CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAACATAGAT TACAAGAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTGGATGC AGGAGGGGTG CGCAAAGAAT TTTTCTTGCT CATCATGAGG 
GAATTATTGG ATCCTAAATA CGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTCCATT TGATTGGTGT TATCTGTGGC 
TTAGCAATTT ATAATTGTAC CATTGTGGAC CTCCATTTTC CTTTGGCTTT ATATAAGAAA 
CTACTGAAAA AGAAGCCATC CTTGGATGAT TTGAAAGAAC TAATGCCTGA TGTTGGGAGA 
AGCATGCAAC AGTTACTGGA TTATCCAGAA GATGACATAG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAAGAGTTTG TCGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTATTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AAGTCCTTCT GCTCTTTCAG CCTAATGAAC TACAAGCAAT GGTCATTGGA 
AATACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAATATTGG 
GCAGAACATC CTACGATAAA AATTTTTTGG GAAGTATTTC ACGAATTACC ATTGGAAAAG 
AAGAAACAGT TTCTGTTATT TTTGACAGGT AGTGATCGCA TTCCTATTCT TGGTATGAAG 
AGTCTGAAAC TAGTCATCCA GTCCACAGGA GGTGGTGAGG AGTATCTCCC AGTTTCCCAT 
ACTTGTTTTA ATCTTCTGGA TCTTCCAAAA TATACAGAAA AAGAAACTCT ACGCTCTAAA 
CTGATCCAAG CTATTGATCA CAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_001278186.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265115.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.

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CloneID	OHu00507
Clone ID Related Accession (Same CDS sequence)	NM_001278186.2 , NM_001278186.1
Accession Version	NM_001278186.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, AY650032.1 and BC018114.1. On Aug 31, 2013 this sequence version replaced XM_005269686.1. Transcript Variant: This variant (4) lacks two alternate exons compared to variant 1, and one causes a frameshift. These differences cause translation initiation at a downstream AUG and result in an isoform (d) with a shorter and distinct N-terminus and lacking an alternate internal segment, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY650032.1, BC015043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAAA TGGAAGGTGG AGTGTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGC 
CATAATTCTA CCAGTCATGA AATAAACCCA AGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTCACTGAGA TTGCTTGTGG ACGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTACTCTT TTGGGCTTGG TGGTAATGGG CAGCTGGGAA CCGGTTCAAC AAGCAACAGG 
AAAAGCCCCT TTACTGTAAA AGGAAATTGG TACCCCTATA ATGGGCAGTG TCTACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTAAAA AGAATTTTCT CAGGGGGAGA TCAAAGCTTT 
TCACATTACT CTAGTCCCCA GAACTGTGGG CCACCAGATG ACTTCAGATG TCCCAATCCG 
ACAAAGCAGA TCTGGACAGT GAATGAAGCT CTAATTCAGA AATGGCTGAG CTATCCTTCT 
GGAAGGTTTC CTGTGGAGAT AGCCAATGAG ATAGATGGAA CGTTTTCTTC CTCTGGTTGC 
CTAAATGGAA GTTTTTTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG TACCAGATTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCGCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGCTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGTAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAGACCG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ATCAGGCCCA CAGGCAGAAT GTCTCCTCTC TTTTTCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTGGTG CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAACATAGAT TACAAGAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTGGATGC AGGAGGGGTG CGCAAAGAAT TTTTCTTGCT CATCATGAGG 
GAATTATTGG ATCCTAAATA CGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTCCATT TGATTGGTGT TATCTGTGGC 
TTAGCAATTT ATAATTGTAC CATTGTGGAC CTCCATTTTC CTTTGGCTTT ATATAAGAAA 
CTACTGAAAA AGAAGCCATC CTTGGATGAT TTGAAAGAAC TAATGCCTGA TGTTGGGAGA 
AGCATGCAAC AGTTACTGGA TTATCCAGAA GATGACATAG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAAGAGTTTG TCGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTATTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AAGTCCTTCT GCTCTTTCAG CCTAATGAAC TACAAGCAAT GGTCATTGGA 
AATACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAATATTGG 
GCAGAACATC CTACGATAAA AATTTTTTGG GAAGTATTTC ACGAATTACC ATTGGAAAAG 
AAGAAACAGT TTCTGTTATT TTTGACAGGT AGTGATCGCA TTCCTATTCT TGGTATGAAG 
AGTCTGAAAC TAGTCATCCA GTCCACAGGA GGTGGTGAGG AGTATCTCCC AGTTTCCCAT 
ACTTGTTTTA ATCTTCTGGA TCTTCCAAAA TATACAGAAA AAGAAACTCT ACGCTCTAAA 
CTGATCCAAG CTATTGATCA CAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265115.1
CDS	825..3209
Translation	MMKMEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_001278186.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278186.1

ORF Insert Sequence:

ATGATGAAAA TGGAAGGTGG AGTGTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGC 
CATAATTCTA CCAGTCATGA AATAAACCCA AGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTCACTGAGA TTGCTTGTGG ACGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTACTCTT TTGGGCTTGG TGGTAATGGG CAGCTGGGAA CCGGTTCAAC AAGCAACAGG 
AAAAGCCCCT TTACTGTAAA AGGAAATTGG TACCCCTATA ATGGGCAGTG TCTACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTAAAA AGAATTTTCT CAGGGGGAGA TCAAAGCTTT 
TCACATTACT CTAGTCCCCA GAACTGTGGG CCACCAGATG ACTTCAGATG TCCCAATCCG 
ACAAAGCAGA TCTGGACAGT GAATGAAGCT CTAATTCAGA AATGGCTGAG CTATCCTTCT 
GGAAGGTTTC CTGTGGAGAT AGCCAATGAG ATAGATGGAA CGTTTTCTTC CTCTGGTTGC 
CTAAATGGAA GTTTTTTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG TACCAGATTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCGCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGCTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGTAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAGACCG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ATCAGGCCCA CAGGCAGAAT GTCTCCTCTC TTTTTCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTGGTG CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAACATAGAT TACAAGAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTGGATGC AGGAGGGGTG CGCAAAGAAT TTTTCTTGCT CATCATGAGG 
GAATTATTGG ATCCTAAATA CGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTCCATT TGATTGGTGT TATCTGTGGC 
TTAGCAATTT ATAATTGTAC CATTGTGGAC CTCCATTTTC CTTTGGCTTT ATATAAGAAA 
CTACTGAAAA AGAAGCCATC CTTGGATGAT TTGAAAGAAC TAATGCCTGA TGTTGGGAGA 
AGCATGCAAC AGTTACTGGA TTATCCAGAA GATGACATAG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAAGAGTTTG TCGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTATTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AAGTCCTTCT GCTCTTTCAG CCTAATGAAC TACAAGCAAT GGTCATTGGA 
AATACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAATATTGG 
GCAGAACATC CTACGATAAA AATTTTTTGG GAAGTATTTC ACGAATTACC ATTGGAAAAG 
AAGAAACAGT TTCTGTTATT TTTGACAGGT AGTGATCGCA TTCCTATTCT TGGTATGAAG 
AGTCTGAAAC TAGTCATCCA GTCCACAGGA GGTGGTGAGG AGTATCTCCC AGTTTCCCAT 
ACTTGTTTTA ATCTTCTGGA TCTTCCAAAA TATACAGAAA AAGAAACTCT ACGCTCTAAA 
CTGATCCAAG CTATTGATCA CAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539596.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537898.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.

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CloneID	OHu66795
Clone ID Related Accession (Same CDS sequence)	XM_011539596.2
Accession Version	XM_011539596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGAAAT 
ATTAAAAGTT TGTCAGATAT CCAGATTGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT 
GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT 
TTAGGTACTG ACTGTAAAAA GCAAACTTCA CCGCAGCTGC TTAAGTCTTT GCTTGGAATC 
CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT 
ATCTTTGGAT GGGGACGCAA CAAGTTTGGT CAGCTAGGTC TTAATGATGA AAATGATAGG 
TATGTTCCTA ATTTACTAAA GTCACTAAGA TCTCAGAAAA TAGTTTATAT TTGTTGTGGA 
GAAGATCATA CTGCTGCTCT AACCAAGGAA GGTGGAGTGT TTACTTTTGG AGCTGGAGGG 
TATGGTCAGT TGGGCCATAA TTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA 
CTTATGGGAA GCATTGTCAC TGAGATTGCT TGTGGACGGC AGCACACTTC TGCTTTTGTT 
CCTTCATCAG GACGAATTTA CTCTTTTGGG CTTGGTGGTA ATGGGCAGCT GGGAACCGGT 
TCAACAAGCA ACAGGAAAAG CCCCTTTACT GTAAAAGGAA ATTGGTACCC CTATAATGGG 
CAGTGTCTAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TAAAAAGAAT TTTCTCAGGG 
GGAGATCAAA GCTTTTCACA TTACTCTAGT CCCCAGAACT GTGGGCCACC AGATGACTTC 
AGATGTCCCA ATCCGACAAA GCAGATCTGG ACAGTGAATG AAGCTCTAAT TCAGAAATGG 
CTGAGCTATC CTTCTGGAAG GTTTCCTGTG GAGATAGCCA ATGAGATAGA TGGAACGTTT 
TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA 
ACAGGTACCA GATTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT 
ATACAACCTG ATCATCCGCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT 
ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA 
CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT 
ACAGCTCTTG TGAACCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA 
CTTGAACCTC CACTATTCCT CAAGATAGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT 
TTGAAACTCT ACAAGATCGG TATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT 
CATACTGCAT TAAAGGTTTT AGAAATACTA CATAGGGTAA ATGAGAAAAT GGGACAGATT 
ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGAAATGAT 
TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG 
TTGGCAGATA TCCCTGTTAC AATCTGTACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAGACCGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA TCAGGCCCAC 
AGGCAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTGGTGC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AACATAGATT ACAAGAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTGGATGCA 
GGAGGGGTGC GCAAAGAATT TTTCTTGCTC ATCATGAGGG AATTATTGGA TCCTAAATAC 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTCCATTT GATTGGTGTT ATCTGTGGCT TAGCAATTTA TAATTGTACC 
ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TACTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAAGAACT AATGCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAT 
TATCCAGAAG ATGACATAGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AAGAGTTTGT CGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCTG 
CTCTTTCAGC CTAATGAACT ACAAGCAATG GTCATTGGAA ATACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAATATTGGG CAGAACATCC TACGATAAAA 
ATTTTTTGGG AAGTATTTCA CGAATTACCA TTGGAAAAGA AGAAACAGTT TCTGTTATTT 
TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTGAAACT AGTCATCCAG 
TCCACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CTTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ATACAGAAAA AGAAACTCTA CGCTCTAAAC TGATCCAAGC TATTGATCAC 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537898.1
CDS	610..3573
Translation	MAAHVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539596.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539596.2

ORF Insert Sequence:

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGAAAT 
ATTAAAAGTT TGTCAGATAT CCAGATTGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT 
GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT 
TTAGGTACTG ACTGTAAAAA GCAAACTTCA CCGCAGCTGC TTAAGTCTTT GCTTGGAATC 
CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT 
ATCTTTGGAT GGGGACGCAA CAAGTTTGGT CAGCTAGGTC TTAATGATGA AAATGATAGG 
TATGTTCCTA ATTTACTAAA GTCACTAAGA TCTCAGAAAA TAGTTTATAT TTGTTGTGGA 
GAAGATCATA CTGCTGCTCT AACCAAGGAA GGTGGAGTGT TTACTTTTGG AGCTGGAGGG 
TATGGTCAGT TGGGCCATAA TTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA 
CTTATGGGAA GCATTGTCAC TGAGATTGCT TGTGGACGGC AGCACACTTC TGCTTTTGTT 
CCTTCATCAG GACGAATTTA CTCTTTTGGG CTTGGTGGTA ATGGGCAGCT GGGAACCGGT 
TCAACAAGCA ACAGGAAAAG CCCCTTTACT GTAAAAGGAA ATTGGTACCC CTATAATGGG 
CAGTGTCTAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TAAAAAGAAT TTTCTCAGGG 
GGAGATCAAA GCTTTTCACA TTACTCTAGT CCCCAGAACT GTGGGCCACC AGATGACTTC 
AGATGTCCCA ATCCGACAAA GCAGATCTGG ACAGTGAATG AAGCTCTAAT TCAGAAATGG 
CTGAGCTATC CTTCTGGAAG GTTTCCTGTG GAGATAGCCA ATGAGATAGA TGGAACGTTT 
TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA 
ACAGGTACCA GATTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT 
ATACAACCTG ATCATCCGCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT 
ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA 
CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT 
ACAGCTCTTG TGAACCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA 
CTTGAACCTC CACTATTCCT CAAGATAGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT 
TTGAAACTCT ACAAGATCGG TATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT 
CATACTGCAT TAAAGGTTTT AGAAATACTA CATAGGGTAA ATGAGAAAAT GGGACAGATT 
ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGAAATGAT 
TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG 
TTGGCAGATA TCCCTGTTAC AATCTGTACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAGACCGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA TCAGGCCCAC 
AGGCAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTGGTGC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AACATAGATT ACAAGAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTGGATGCA 
GGAGGGGTGC GCAAAGAATT TTTCTTGCTC ATCATGAGGG AATTATTGGA TCCTAAATAC 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTCCATTT GATTGGTGTT ATCTGTGGCT TAGCAATTTA TAATTGTACC 
ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TACTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAAGAACT AATGCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAT 
TATCCAGAAG ATGACATAGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AAGAGTTTGT CGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCTG 
CTCTTTCAGC CTAATGAACT ACAAGCAATG GTCATTGGAA ATACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAATATTGGG CAGAACATCC TACGATAAAA 
ATTTTTTGGG AAGTATTTCA CGAATTACCA TTGGAAAAGA AGAAACAGTT TCTGTTATTT 
TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTGAAACT AGTCATCCAG 
TCCACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CTTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ATACAGAAAA AGAAACTCTA CGCTCTAAAC TGATCCAAGC TATTGATCAC 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539597.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537899.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu66796
Clone ID Related Accession (Same CDS sequence)	XM_011539597.3 , XM_011539597.2
Accession Version	XM_011539597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTAAA TGAGAAAATG GGACAGATTA TACAGTATGA TAAATTTTAT 
ATACATGAAG TACAAGAATT GATAGACATA AGAAATGATT ATATCAACTG GGTCCAACAG 
CAGGCCTATG GAATGGATGT CAACCATGGA TTAACTGAGT TGGCAGATAT CCCTGTTACA 
ATCTGTACAT ATCCATTTGT ATTTGATGCC CAAGCAAAAA CTACTCTGTT ACAGACCGAT 
GCAGTCTTAC AGATGCAGAT GGCTATTGAT CAGGCCCACA GGCAGAATGT CTCCTCTCTT 
TTTCTCCCAG TGATTGAATC TGTGAATCCC TGCTTAATTC TAGTGGTGCG TAGAGAAAAT 
ATTGTAGGAG ATGCAATGGA AGTCCTTAGG AAAACAAAGA ACATAGATTA CAAGAAGCCA 
CTCAAGGTTA TATTTGTTGG AGAAGATGCT GTGGATGCAG GAGGGGTGCG CAAAGAATTT 
TTCTTGCTCA TCATGAGGGA ATTATTGGAT CCTAAATACG GCATGTTTAG GTATTATGAA 
GATTCCAGGC TCATTTGGTT TTCTGATAAG ACATTTGAAG ACAGTGATTT GTTCCATTTG 
ATTGGTGTTA TCTGTGGCTT AGCAATTTAT AATTGTACCA TTGTGGACCT CCATTTTCCT 
TTGGCTTTAT ATAAGAAACT ACTGAAAAAG AAGCCATCCT TGGATGATTT GAAAGAACTA 
ATGCCTGATG TTGGGAGAAG CATGCAACAG TTACTGGATT ATCCAGAAGA TGACATAGAG 
GAAACATTTT GTCTTAATTT TACGATCACA GTTGAAAACT TTGGTGCAAC AGAAGTGAAA 
GAGCTGGTTC TAAATGGTGC AGACACAGCT GTTAACAAAC AAAATCGGCA AGAGTTTGTC 
GATGCTTATG TGGATTACAT ATTCAATAAA TCAGTGGCTT CCTTATTTGA TGCTTTTCAT 
GCGGGCTTTC ATAAGGTCTG TGGAGGAAAA GTCCTTCTGC TCTTTCAGCC TAATGAACTA 
CAAGCAATGG TCATTGGAAA TACAAATTAT GATTGGAAGG AACTGGAAAA GAATACAGAA 
TACAAAGGGG AATATTGGGC AGAACATCCT ACGATAAAAA TTTTTTGGGA AGTATTTCAC 
GAATTACCAT TGGAAAAGAA GAAACAGTTT CTGTTATTTT TGACAGGTAG TGATCGCATT 
CCTATTCTTG GTATGAAGAG TCTGAAACTA GTCATCCAGT CCACAGGAGG TGGTGAGGAG 
TATCTCCCAG TTTCCCATAC TTGTTTTAAT CTTCTGGATC TTCCAAAATA TACAGAAAAA 
GAAACTCTAC GCTCTAAACT GATCCAAGCT ATTGATCACA ATGAAGGCTT CAGTTTAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537899.1
CDS	290..3172
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539597.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539597.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTAAA TGAGAAAATG GGACAGATTA TACAGTATGA TAAATTTTAT 
ATACATGAAG TACAAGAATT GATAGACATA AGAAATGATT ATATCAACTG GGTCCAACAG 
CAGGCCTATG GAATGGATGT CAACCATGGA TTAACTGAGT TGGCAGATAT CCCTGTTACA 
ATCTGTACAT ATCCATTTGT ATTTGATGCC CAAGCAAAAA CTACTCTGTT ACAGACCGAT 
GCAGTCTTAC AGATGCAGAT GGCTATTGAT CAGGCCCACA GGCAGAATGT CTCCTCTCTT 
TTTCTCCCAG TGATTGAATC TGTGAATCCC TGCTTAATTC TAGTGGTGCG TAGAGAAAAT 
ATTGTAGGAG ATGCAATGGA AGTCCTTAGG AAAACAAAGA ACATAGATTA CAAGAAGCCA 
CTCAAGGTTA TATTTGTTGG AGAAGATGCT GTGGATGCAG GAGGGGTGCG CAAAGAATTT 
TTCTTGCTCA TCATGAGGGA ATTATTGGAT CCTAAATACG GCATGTTTAG GTATTATGAA 
GATTCCAGGC TCATTTGGTT TTCTGATAAG ACATTTGAAG ACAGTGATTT GTTCCATTTG 
ATTGGTGTTA TCTGTGGCTT AGCAATTTAT AATTGTACCA TTGTGGACCT CCATTTTCCT 
TTGGCTTTAT ATAAGAAACT ACTGAAAAAG AAGCCATCCT TGGATGATTT GAAAGAACTA 
ATGCCTGATG TTGGGAGAAG CATGCAACAG TTACTGGATT ATCCAGAAGA TGACATAGAG 
GAAACATTTT GTCTTAATTT TACGATCACA GTTGAAAACT TTGGTGCAAC AGAAGTGAAA 
GAGCTGGTTC TAAATGGTGC AGACACAGCT GTTAACAAAC AAAATCGGCA AGAGTTTGTC 
GATGCTTATG TGGATTACAT ATTCAATAAA TCAGTGGCTT CCTTATTTGA TGCTTTTCAT 
GCGGGCTTTC ATAAGGTCTG TGGAGGAAAA GTCCTTCTGC TCTTTCAGCC TAATGAACTA 
CAAGCAATGG TCATTGGAAA TACAAATTAT GATTGGAAGG AACTGGAAAA GAATACAGAA 
TACAAAGGGG AATATTGGGC AGAACATCCT ACGATAAAAA TTTTTTGGGA AGTATTTCAC 
GAATTACCAT TGGAAAAGAA GAAACAGTTT CTGTTATTTT TGACAGGTAG TGATCGCATT 
CCTATTCTTG GTATGAAGAG TCTGAAACTA GTCATCCAGT CCACAGGAGG TGGTGAGGAG 
TATCTCCCAG TTTCCCATAC TTGTTTTAAT CTTCTGGATC TTCCAAAATA TACAGAAAAA 
GAAACTCTAC GCTCTAAACT GATCCAAGCT ATTGATCACA ATGAAGGCTT CAGTTTAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539597.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537899.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu66796
Clone ID Related Accession (Same CDS sequence)	XM_011539597.3 , XM_011539597.2
Accession Version	XM_011539597.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTAAA TGAGAAAATG GGACAGATTA TACAGTATGA TAAATTTTAT 
ATACATGAAG TACAAGAATT GATAGACATA AGAAATGATT ATATCAACTG GGTCCAACAG 
CAGGCCTATG GAATGGATGT CAACCATGGA TTAACTGAGT TGGCAGATAT CCCTGTTACA 
ATCTGTACAT ATCCATTTGT ATTTGATGCC CAAGCAAAAA CTACTCTGTT ACAGACCGAT 
GCAGTCTTAC AGATGCAGAT GGCTATTGAT CAGGCCCACA GGCAGAATGT CTCCTCTCTT 
TTTCTCCCAG TGATTGAATC TGTGAATCCC TGCTTAATTC TAGTGGTGCG TAGAGAAAAT 
ATTGTAGGAG ATGCAATGGA AGTCCTTAGG AAAACAAAGA ACATAGATTA CAAGAAGCCA 
CTCAAGGTTA TATTTGTTGG AGAAGATGCT GTGGATGCAG GAGGGGTGCG CAAAGAATTT 
TTCTTGCTCA TCATGAGGGA ATTATTGGAT CCTAAATACG GCATGTTTAG GTATTATGAA 
GATTCCAGGC TCATTTGGTT TTCTGATAAG ACATTTGAAG ACAGTGATTT GTTCCATTTG 
ATTGGTGTTA TCTGTGGCTT AGCAATTTAT AATTGTACCA TTGTGGACCT CCATTTTCCT 
TTGGCTTTAT ATAAGAAACT ACTGAAAAAG AAGCCATCCT TGGATGATTT GAAAGAACTA 
ATGCCTGATG TTGGGAGAAG CATGCAACAG TTACTGGATT ATCCAGAAGA TGACATAGAG 
GAAACATTTT GTCTTAATTT TACGATCACA GTTGAAAACT TTGGTGCAAC AGAAGTGAAA 
GAGCTGGTTC TAAATGGTGC AGACACAGCT GTTAACAAAC AAAATCGGCA AGAGTTTGTC 
GATGCTTATG TGGATTACAT ATTCAATAAA TCAGTGGCTT CCTTATTTGA TGCTTTTCAT 
GCGGGCTTTC ATAAGGTCTG TGGAGGAAAA GTCCTTCTGC TCTTTCAGCC TAATGAACTA 
CAAGCAATGG TCATTGGAAA TACAAATTAT GATTGGAAGG AACTGGAAAA GAATACAGAA 
TACAAAGGGG AATATTGGGC AGAACATCCT ACGATAAAAA TTTTTTGGGA AGTATTTCAC 
GAATTACCAT TGGAAAAGAA GAAACAGTTT CTGTTATTTT TGACAGGTAG TGATCGCATT 
CCTATTCTTG GTATGAAGAG TCTGAAACTA GTCATCCAGT CCACAGGAGG TGGTGAGGAG 
TATCTCCCAG TTTCCCATAC TTGTTTTAAT CTTCTGGATC TTCCAAAATA TACAGAAAAA 
GAAACTCTAC GCTCTAAACT GATCCAAGCT ATTGATCACA ATGAAGGCTT CAGTTTAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537899.1
CDS	314..3196
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539597.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539597.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTAAA TGAGAAAATG GGACAGATTA TACAGTATGA TAAATTTTAT 
ATACATGAAG TACAAGAATT GATAGACATA AGAAATGATT ATATCAACTG GGTCCAACAG 
CAGGCCTATG GAATGGATGT CAACCATGGA TTAACTGAGT TGGCAGATAT CCCTGTTACA 
ATCTGTACAT ATCCATTTGT ATTTGATGCC CAAGCAAAAA CTACTCTGTT ACAGACCGAT 
GCAGTCTTAC AGATGCAGAT GGCTATTGAT CAGGCCCACA GGCAGAATGT CTCCTCTCTT 
TTTCTCCCAG TGATTGAATC TGTGAATCCC TGCTTAATTC TAGTGGTGCG TAGAGAAAAT 
ATTGTAGGAG ATGCAATGGA AGTCCTTAGG AAAACAAAGA ACATAGATTA CAAGAAGCCA 
CTCAAGGTTA TATTTGTTGG AGAAGATGCT GTGGATGCAG GAGGGGTGCG CAAAGAATTT 
TTCTTGCTCA TCATGAGGGA ATTATTGGAT CCTAAATACG GCATGTTTAG GTATTATGAA 
GATTCCAGGC TCATTTGGTT TTCTGATAAG ACATTTGAAG ACAGTGATTT GTTCCATTTG 
ATTGGTGTTA TCTGTGGCTT AGCAATTTAT AATTGTACCA TTGTGGACCT CCATTTTCCT 
TTGGCTTTAT ATAAGAAACT ACTGAAAAAG AAGCCATCCT TGGATGATTT GAAAGAACTA 
ATGCCTGATG TTGGGAGAAG CATGCAACAG TTACTGGATT ATCCAGAAGA TGACATAGAG 
GAAACATTTT GTCTTAATTT TACGATCACA GTTGAAAACT TTGGTGCAAC AGAAGTGAAA 
GAGCTGGTTC TAAATGGTGC AGACACAGCT GTTAACAAAC AAAATCGGCA AGAGTTTGTC 
GATGCTTATG TGGATTACAT ATTCAATAAA TCAGTGGCTT CCTTATTTGA TGCTTTTCAT 
GCGGGCTTTC ATAAGGTCTG TGGAGGAAAA GTCCTTCTGC TCTTTCAGCC TAATGAACTA 
CAAGCAATGG TCATTGGAAA TACAAATTAT GATTGGAAGG AACTGGAAAA GAATACAGAA 
TACAAAGGGG AATATTGGGC AGAACATCCT ACGATAAAAA TTTTTTGGGA AGTATTTCAC 
GAATTACCAT TGGAAAAGAA GAAACAGTTT CTGTTATTTT TGACAGGTAG TGATCGCATT 
CCTATTCTTG GTATGAAGAG TCTGAAACTA GTCATCCAGT CCACAGGAGG TGGTGAGGAG 
TATCTCCCAG TTTCCCATAC TTGTTTTAAT CTTCTGGATC TTCCAAAATA TACAGAAAAA 
GAAACTCTAC GCTCTAAACT GATCCAAGCT ATTGATCACA ATGAAGGCTT CAGTTTAATA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447932.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303700.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu106447
Clone ID Related Accession (Same CDS sequence)	XM_024447932.1
Accession Version	XM_024447932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GGATGTCAAC CATGGATTAA CTGAGTTGGC AGATATCCCT 
GTTACAATCT GTACATATCC ATTTGTATTT GATGCCCAAG CAAAAACTAC TCTGTTACAG 
ACCGATGCAG TCTTACAGAT GCAGATGGCT ATTGATCAGG CCCACAGGCA GAATGTCTCC 
TCTCTTTTTC TCCCAGTGAT TGAATCTGTG AATCCCTGCT TAATTCTAGT GGTGCGTAGA 
GAAAATATTG TAGGAGATGC AATGGAAGTC CTTAGGAAAA CAAAGAACAT AGATTACAAG 
AAGCCACTCA AGGTTATATT TGTTGGAGAA GATGCTGTGG ATGCAGGAGG GGTGCGCAAA 
GAATTTTTCT TGCTCATCAT GAGGGAATTA TTGGATCCTA AATACGGCAT GTTTAGGTAT 
TATGAAGATT CCAGGCTCAT TTGGTTTTCT GATAAGACAT TTGAAGACAG TGATTTGTTC 
CATTTGATTG GTGTTATCTG TGGCTTAGCA ATTTATAATT GTACCATTGT GGACCTCCAT 
TTTCCTTTGG CTTTATATAA GAAACTACTG AAAAAGAAGC CATCCTTGGA TGATTTGAAA 
GAACTAATGC CTGATGTTGG GAGAAGCATG CAACAGTTAC TGGATTATCC AGAAGATGAC 
ATAGAGGAAA CATTTTGTCT TAATTTTACG ATCACAGTTG AAAACTTTGG TGCAACAGAA 
GTGAAAGAGC TGGTTCTAAA TGGTGCAGAC ACAGCTGTTA ACAAACAAAA TCGGCAAGAG 
TTTGTCGATG CTTATGTGGA TTACATATTC AATAAATCAG TGGCTTCCTT ATTTGATGCT 
TTTCATGCGG GCTTTCATAA GGTCTGTGGA GGAAAAGTCC TTCTGCTCTT TCAGCCTAAT 
GAACTACAAG CAATGGTCAT TGGAAATACA AATTATGATT GGAAGGAACT GGAAAAGAAT 
ACAGAATACA AAGGGGAATA TTGGGCAGAA CATCCTACGA TAAAAATTTT TTGGGAAGTA 
TTTCACGAAT TACCATTGGA AAAGAAGAAA CAGTTTCTGT TATTTTTGAC AGGTAGTGAT 
CGCATTCCTA TTCTTGGTAT GAAGAGTCTG AAACTAGTCA TCCAGTCCAC AGGAGGTGGT 
GAGGAGTATC TCCCAGTTTC CCATACTTGT TTTAATCTTC TGGATCTTCC AAAATATACA 
GAAAAAGAAA CTCTACGCTC TAAACTGATC CAAGCTATTG ATCACAATGA AGGCTTCAGT 
TTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303700.1
CDS	2317..3945
Translation	MSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447932.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447932.1

ORF Insert Sequence:

ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTGAAC 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TAGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGTATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TACTACATAG GGTAAATGAG AAAATGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGAA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GGATGTCAAC CATGGATTAA CTGAGTTGGC AGATATCCCT 
GTTACAATCT GTACATATCC ATTTGTATTT GATGCCCAAG CAAAAACTAC TCTGTTACAG 
ACCGATGCAG TCTTACAGAT GCAGATGGCT ATTGATCAGG CCCACAGGCA GAATGTCTCC 
TCTCTTTTTC TCCCAGTGAT TGAATCTGTG AATCCCTGCT TAATTCTAGT GGTGCGTAGA 
GAAAATATTG TAGGAGATGC AATGGAAGTC CTTAGGAAAA CAAAGAACAT AGATTACAAG 
AAGCCACTCA AGGTTATATT TGTTGGAGAA GATGCTGTGG ATGCAGGAGG GGTGCGCAAA 
GAATTTTTCT TGCTCATCAT GAGGGAATTA TTGGATCCTA AATACGGCAT GTTTAGGTAT 
TATGAAGATT CCAGGCTCAT TTGGTTTTCT GATAAGACAT TTGAAGACAG TGATTTGTTC 
CATTTGATTG GTGTTATCTG TGGCTTAGCA ATTTATAATT GTACCATTGT GGACCTCCAT 
TTTCCTTTGG CTTTATATAA GAAACTACTG AAAAAGAAGC CATCCTTGGA TGATTTGAAA 
GAACTAATGC CTGATGTTGG GAGAAGCATG CAACAGTTAC TGGATTATCC AGAAGATGAC 
ATAGAGGAAA CATTTTGTCT TAATTTTACG ATCACAGTTG AAAACTTTGG TGCAACAGAA 
GTGAAAGAGC TGGTTCTAAA TGGTGCAGAC ACAGCTGTTA ACAAACAAAA TCGGCAAGAG 
TTTGTCGATG CTTATGTGGA TTACATATTC AATAAATCAG TGGCTTCCTT ATTTGATGCT 
TTTCATGCGG GCTTTCATAA GGTCTGTGGA GGAAAAGTCC TTCTGCTCTT TCAGCCTAAT 
GAACTACAAG CAATGGTCAT TGGAAATACA AATTATGATT GGAAGGAACT GGAAAAGAAT 
ACAGAATACA AAGGGGAATA TTGGGCAGAA CATCCTACGA TAAAAATTTT TTGGGAAGTA 
TTTCACGAAT TACCATTGGA AAAGAAGAAA CAGTTTCTGT TATTTTTGAC AGGTAGTGAT 
CGCATTCCTA TTCTTGGTAT GAAGAGTCTG AAACTAGTCA TCCAGTCCAC AGGAGGTGGT 
GAGGAGTATC TCCCAGTTTC CCATACTTGT TTTAATCTTC TGGATCTTCC AAAATATACA 
GAAAAAGAAA CTCTACGCTC TAAACTGATC CAAGCTATTG ATCACAATGA AGGCTTCAGT 
TTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_017016041.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871530.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu91278
Clone ID Related Accession (Same CDS sequence)	XM_017016041.1 , XM_017016041.2
Accession Version	XM_017016041.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTAAATG AGAAAATGGG ACAGATTATA CAGTATGATA AATTTTATAT ACATGAAGTA 
CAAGAATTGA TAGACATAAG AAATGATTAT ATCAACTGGG TCCAACAGCA GGCCTATGGA 
ATGGATGTCA ACCATGGATT AACTGAGTTG GCAGATATCC CTGTTACAAT CTGTACATAT 
CCATTTGTAT TTGATGCCCA AGCAAAAACT ACTCTGTTAC AGACCGATGC AGTCTTACAG 
ATGCAGATGG CTATTGATCA GGCCCACAGG CAGAATGTCT CCTCTCTTTT TCTCCCAGTG 
ATTGAATCTG TGAATCCCTG CTTAATTCTA GTGGTGCGTA GAGAAAATAT TGTAGGAGAT 
GCAATGGAAG TCCTTAGGAA AACAAAGAAC ATAGATTACA AGAAGCCACT CAAGGTTATA 
TTTGTTGGAG AAGATGCTGT GGATGCAGGA GGGGTGCGCA AAGAATTTTT CTTGCTCATC 
ATGAGGGAAT TATTGGATCC TAAATACGGC ATGTTTAGGT ATTATGAAGA TTCCAGGCTC 
ATTTGGTTTT CTGATAAGAC ATTTGAAGAC AGTGATTTGT TCCATTTGAT TGGTGTTATC 
TGTGGCTTAG CAATTTATAA TTGTACCATT GTGGACCTCC ATTTTCCTTT GGCTTTATAT 
AAGAAACTAC TGAAAAAGAA GCCATCCTTG GATGATTTGA AAGAACTAAT GCCTGATGTT 
GGGAGAAGCA TGCAACAGTT ACTGGATTAT CCAGAAGATG ACATAGAGGA AACATTTTGT 
CTTAATTTTA CGATCACAGT TGAAAACTTT GGTGCAACAG AAGTGAAAGA GCTGGTTCTA 
AATGGTGCAG ACACAGCTGT TAACAAACAA AATCGGCAAG AGTTTGTCGA TGCTTATGTG 
GATTACATAT TCAATAAATC AGTGGCTTCC TTATTTGATG CTTTTCATGC GGGCTTTCAT 
AAGGTCTGTG GAGGAAAAGT CCTTCTGCTC TTTCAGCCTA ATGAACTACA AGCAATGGTC 
ATTGGAAATA CAAATTATGA TTGGAAGGAA CTGGAAAAGA ATACAGAATA CAAAGGGGAA 
TATTGGGCAG AACATCCTAC GATAAAAATT TTTTGGGAAG TATTTCACGA ATTACCATTG 
GAAAAGAAGA AACAGTTTCT GTTATTTTTG ACAGGTAGTG ATCGCATTCC TATTCTTGGT 
ATGAAGAGTC TGAAACTAGT CATCCAGTCC ACAGGAGGTG GTGAGGAGTA TCTCCCAGTT 
TCCCATACTT GTTTTAATCT TCTGGATCTT CCAAAATATA CAGAAAAAGA AACTCTACGC 
TCTAAACTGA TCCAAGCTAT TGATCACAAT GAAGGCTTCA GTTTAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871530.1
CDS	314..3124
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_017016041.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016041.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTAAATG AGAAAATGGG ACAGATTATA CAGTATGATA AATTTTATAT ACATGAAGTA 
CAAGAATTGA TAGACATAAG AAATGATTAT ATCAACTGGG TCCAACAGCA GGCCTATGGA 
ATGGATGTCA ACCATGGATT AACTGAGTTG GCAGATATCC CTGTTACAAT CTGTACATAT 
CCATTTGTAT TTGATGCCCA AGCAAAAACT ACTCTGTTAC AGACCGATGC AGTCTTACAG 
ATGCAGATGG CTATTGATCA GGCCCACAGG CAGAATGTCT CCTCTCTTTT TCTCCCAGTG 
ATTGAATCTG TGAATCCCTG CTTAATTCTA GTGGTGCGTA GAGAAAATAT TGTAGGAGAT 
GCAATGGAAG TCCTTAGGAA AACAAAGAAC ATAGATTACA AGAAGCCACT CAAGGTTATA 
TTTGTTGGAG AAGATGCTGT GGATGCAGGA GGGGTGCGCA AAGAATTTTT CTTGCTCATC 
ATGAGGGAAT TATTGGATCC TAAATACGGC ATGTTTAGGT ATTATGAAGA TTCCAGGCTC 
ATTTGGTTTT CTGATAAGAC ATTTGAAGAC AGTGATTTGT TCCATTTGAT TGGTGTTATC 
TGTGGCTTAG CAATTTATAA TTGTACCATT GTGGACCTCC ATTTTCCTTT GGCTTTATAT 
AAGAAACTAC TGAAAAAGAA GCCATCCTTG GATGATTTGA AAGAACTAAT GCCTGATGTT 
GGGAGAAGCA TGCAACAGTT ACTGGATTAT CCAGAAGATG ACATAGAGGA AACATTTTGT 
CTTAATTTTA CGATCACAGT TGAAAACTTT GGTGCAACAG AAGTGAAAGA GCTGGTTCTA 
AATGGTGCAG ACACAGCTGT TAACAAACAA AATCGGCAAG AGTTTGTCGA TGCTTATGTG 
GATTACATAT TCAATAAATC AGTGGCTTCC TTATTTGATG CTTTTCATGC GGGCTTTCAT 
AAGGTCTGTG GAGGAAAAGT CCTTCTGCTC TTTCAGCCTA ATGAACTACA AGCAATGGTC 
ATTGGAAATA CAAATTATGA TTGGAAGGAA CTGGAAAAGA ATACAGAATA CAAAGGGGAA 
TATTGGGCAG AACATCCTAC GATAAAAATT TTTTGGGAAG TATTTCACGA ATTACCATTG 
GAAAAGAAGA AACAGTTTCT GTTATTTTTG ACAGGTAGTG ATCGCATTCC TATTCTTGGT 
ATGAAGAGTC TGAAACTAGT CATCCAGTCC ACAGGAGGTG GTGAGGAGTA TCTCCCAGTT 
TCCCATACTT GTTTTAATCT TCTGGATCTT CCAAAATATA CAGAAAAAGA AACTCTACGC 
TCTAAACTGA TCCAAGCTAT TGATCACAAT GAAGGCTTCA GTTTAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_017016041.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871530.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.

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CloneID	OHu91278
Clone ID Related Accession (Same CDS sequence)	XM_017016041.1 , XM_017016041.2
Accession Version	XM_017016041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTAAATG AGAAAATGGG ACAGATTATA CAGTATGATA AATTTTATAT ACATGAAGTA 
CAAGAATTGA TAGACATAAG AAATGATTAT ATCAACTGGG TCCAACAGCA GGCCTATGGA 
ATGGATGTCA ACCATGGATT AACTGAGTTG GCAGATATCC CTGTTACAAT CTGTACATAT 
CCATTTGTAT TTGATGCCCA AGCAAAAACT ACTCTGTTAC AGACCGATGC AGTCTTACAG 
ATGCAGATGG CTATTGATCA GGCCCACAGG CAGAATGTCT CCTCTCTTTT TCTCCCAGTG 
ATTGAATCTG TGAATCCCTG CTTAATTCTA GTGGTGCGTA GAGAAAATAT TGTAGGAGAT 
GCAATGGAAG TCCTTAGGAA AACAAAGAAC ATAGATTACA AGAAGCCACT CAAGGTTATA 
TTTGTTGGAG AAGATGCTGT GGATGCAGGA GGGGTGCGCA AAGAATTTTT CTTGCTCATC 
ATGAGGGAAT TATTGGATCC TAAATACGGC ATGTTTAGGT ATTATGAAGA TTCCAGGCTC 
ATTTGGTTTT CTGATAAGAC ATTTGAAGAC AGTGATTTGT TCCATTTGAT TGGTGTTATC 
TGTGGCTTAG CAATTTATAA TTGTACCATT GTGGACCTCC ATTTTCCTTT GGCTTTATAT 
AAGAAACTAC TGAAAAAGAA GCCATCCTTG GATGATTTGA AAGAACTAAT GCCTGATGTT 
GGGAGAAGCA TGCAACAGTT ACTGGATTAT CCAGAAGATG ACATAGAGGA AACATTTTGT 
CTTAATTTTA CGATCACAGT TGAAAACTTT GGTGCAACAG AAGTGAAAGA GCTGGTTCTA 
AATGGTGCAG ACACAGCTGT TAACAAACAA AATCGGCAAG AGTTTGTCGA TGCTTATGTG 
GATTACATAT TCAATAAATC AGTGGCTTCC TTATTTGATG CTTTTCATGC GGGCTTTCAT 
AAGGTCTGTG GAGGAAAAGT CCTTCTGCTC TTTCAGCCTA ATGAACTACA AGCAATGGTC 
ATTGGAAATA CAAATTATGA TTGGAAGGAA CTGGAAAAGA ATACAGAATA CAAAGGGGAA 
TATTGGGCAG AACATCCTAC GATAAAAATT TTTTGGGAAG TATTTCACGA ATTACCATTG 
GAAAAGAAGA AACAGTTTCT GTTATTTTTG ACAGGTAGTG ATCGCATTCC TATTCTTGGT 
ATGAAGAGTC TGAAACTAGT CATCCAGTCC ACAGGAGGTG GTGAGGAGTA TCTCCCAGTT 
TCCCATACTT GTTTTAATCT TCTGGATCTT CCAAAATATA CAGAAAAAGA AACTCTACGC 
TCTAAACTGA TCCAAGCTAT TGATCACAAT GAAGGCTTCA GTTTAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871530.1
CDS	290..3100
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_017016041.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016041.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTAAATG AGAAAATGGG ACAGATTATA CAGTATGATA AATTTTATAT ACATGAAGTA 
CAAGAATTGA TAGACATAAG AAATGATTAT ATCAACTGGG TCCAACAGCA GGCCTATGGA 
ATGGATGTCA ACCATGGATT AACTGAGTTG GCAGATATCC CTGTTACAAT CTGTACATAT 
CCATTTGTAT TTGATGCCCA AGCAAAAACT ACTCTGTTAC AGACCGATGC AGTCTTACAG 
ATGCAGATGG CTATTGATCA GGCCCACAGG CAGAATGTCT CCTCTCTTTT TCTCCCAGTG 
ATTGAATCTG TGAATCCCTG CTTAATTCTA GTGGTGCGTA GAGAAAATAT TGTAGGAGAT 
GCAATGGAAG TCCTTAGGAA AACAAAGAAC ATAGATTACA AGAAGCCACT CAAGGTTATA 
TTTGTTGGAG AAGATGCTGT GGATGCAGGA GGGGTGCGCA AAGAATTTTT CTTGCTCATC 
ATGAGGGAAT TATTGGATCC TAAATACGGC ATGTTTAGGT ATTATGAAGA TTCCAGGCTC 
ATTTGGTTTT CTGATAAGAC ATTTGAAGAC AGTGATTTGT TCCATTTGAT TGGTGTTATC 
TGTGGCTTAG CAATTTATAA TTGTACCATT GTGGACCTCC ATTTTCCTTT GGCTTTATAT 
AAGAAACTAC TGAAAAAGAA GCCATCCTTG GATGATTTGA AAGAACTAAT GCCTGATGTT 
GGGAGAAGCA TGCAACAGTT ACTGGATTAT CCAGAAGATG ACATAGAGGA AACATTTTGT 
CTTAATTTTA CGATCACAGT TGAAAACTTT GGTGCAACAG AAGTGAAAGA GCTGGTTCTA 
AATGGTGCAG ACACAGCTGT TAACAAACAA AATCGGCAAG AGTTTGTCGA TGCTTATGTG 
GATTACATAT TCAATAAATC AGTGGCTTCC TTATTTGATG CTTTTCATGC GGGCTTTCAT 
AAGGTCTGTG GAGGAAAAGT CCTTCTGCTC TTTCAGCCTA ATGAACTACA AGCAATGGTC 
ATTGGAAATA CAAATTATGA TTGGAAGGAA CTGGAAAAGA ATACAGAATA CAAAGGGGAA 
TATTGGGCAG AACATCCTAC GATAAAAATT TTTTGGGAAG TATTTCACGA ATTACCATTG 
GAAAAGAAGA AACAGTTTCT GTTATTTTTG ACAGGTAGTG ATCGCATTCC TATTCTTGGT 
ATGAAGAGTC TGAAACTAGT CATCCAGTCC ACAGGAGGTG GTGAGGAGTA TCTCCCAGTT 
TCCCATACTT GTTTTAATCT TCTGGATCTT CCAAAATATA CAGAAAAAGA AACTCTACGC 
TCTAAACTGA TCCAAGCTAT TGATCACAAT GAAGGCTTCA GTTTAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_022079.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071362.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.

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CloneID	OHu31116
Clone ID Related Accession (Same CDS sequence)	XM_024447929.1 , XM_024447930.1 , NM_022079.2 , NM_022079.3
Accession Version	NM_022079.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB459623.1, AY221963.1, BC015043.1 and BC018114.1. On Apr 19, 2007 this sequence version replaced NM_022079.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.787.1, SRR1660807.37710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071362.1
CDS	312..3485
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_022079.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022079.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( NM_022079.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071362.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.

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CloneID	OHu31116
Clone ID Related Accession (Same CDS sequence)	XM_024447929.1 , XM_024447930.1 , NM_022079.2 , NM_022079.3
Accession Version	NM_022079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY221963.1, BC015043.1 and BC018114.1. On Jun 2, 2019 this sequence version replaced NM_022079.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AY221963.1, SRR1803615.210228.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071362.1
CDS	249..3422
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	NM_022079.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022079.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447929.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303697.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu31116
Clone ID Related Accession (Same CDS sequence)	XM_024447929.1 , XM_024447930.1 , NM_022079.2 , NM_022079.3
Accession Version	XM_024447929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303697.1
CDS	616..3789
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447929.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447930.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303698.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

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CloneID	OHu31116
Clone ID Related Accession (Same CDS sequence)	XM_024447929.1 , XM_024447930.1 , NM_022079.2 , NM_022079.3
Accession Version	XM_024447930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303698.1
CDS	591..3764
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447930.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447930.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATTGTA 
CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGTACTG ACTGTAAAAA GCAAACTTCA 
CCGCAGCTGC TTAAGTCTTT GCTTGGAATC CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAGTTTGGT 
CAGCTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
TCTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCTCT AACCAAGGAA 
GGTGGAGTGT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGCCATAA TTCTACCAGT 
CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TGAGATTGCT 
TGTGGACGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA CTCTTTTGGG 
CTTGGTGGTA ATGGGCAGCT GGGAACCGGT TCAACAAGCA ACAGGAAAAG CCCCTTTACT 
GTAAAAGGAA ATTGGTACCC CTATAATGGG CAGTGTCTAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TAAAAAGAAT TTTCTCAGGG GGAGATCAAA GCTTTTCACA TTACTCTAGT 
CCCCAGAACT GTGGGCCACC AGATGACTTC AGATGTCCCA ATCCGACAAA GCAGATCTGG 
ACAGTGAATG AAGCTCTAAT TCAGAAATGG CTGAGCTATC CTTCTGGAAG GTTTCCTGTG 
GAGATAGCCA ATGAGATAGA TGGAACGTTT TCTTCCTCTG GTTGCCTAAA TGGAAGTTTT 
TTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGTACCA GATTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCGCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATAGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG TATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATACTA 
CATAGGGTAA ATGAGAAAAT GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGAAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGTACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAGACCGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA TCAGGCCCAC AGGCAGAATG TCTCCTCTCT TTTTCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTGGTGC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AACATAGATT ACAAGAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTGGATGCA GGAGGGGTGC GCAAAGAATT TTTCTTGCTC 
ATCATGAGGG AATTATTGGA TCCTAAATAC GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTCCATTT GATTGGTGTT 
ATCTGTGGCT TAGCAATTTA TAATTGTACC ATTGTGGACC TCCATTTTCC TTTGGCTTTA 
TATAAGAAAC TACTGAAAAA GAAGCCATCC TTGGATGATT TGAAAGAACT AATGCCTGAT 
GTTGGGAGAA GCATGCAACA GTTACTGGAT TATCCAGAAG ATGACATAGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AAGAGTTTGT CGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA AGTCCTTCTG CTCTTTCAGC CTAATGAACT ACAAGCAATG 
GTCATTGGAA ATACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAATATTGGG CAGAACATCC TACGATAAAA ATTTTTTGGG AAGTATTTCA CGAATTACCA 
TTGGAAAAGA AGAAACAGTT TCTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT 
GGTATGAAGA GTCTGAAACT AGTCATCCAG TCCACAGGAG GTGGTGAGGA GTATCTCCCA 
GTTTCCCATA CTTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGAAAA AGAAACTCTA 
CGCTCTAAAC TGATCCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447927.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303695.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66792
Clone ID Related Accession (Same CDS sequence)	XM_024447927.1 , XM_011539592.2 , XM_024447928.1 , XM_011539592.3
Accession Version	XM_024447927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303695.1
CDS	592..3837
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447927.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447927.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539592.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537894.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66792
Clone ID Related Accession (Same CDS sequence)	XM_024447927.1 , XM_011539592.2 , XM_024447928.1 , XM_011539592.3
Accession Version	XM_011539592.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537894.1
CDS	314..3559
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539592.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539592.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_024447928.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303696.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu66792
Clone ID Related Accession (Same CDS sequence)	XM_024447927.1 , XM_011539592.2 , XM_024447928.1 , XM_011539592.3
Accession Version	XM_024447928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303696.1
CDS	269..3514
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_024447928.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447928.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539592.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537894.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66792
Clone ID Related Accession (Same CDS sequence)	XM_024447927.1 , XM_011539592.2 , XM_024447928.1 , XM_011539592.3
Accession Version	XM_011539592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537894.1
CDS	290..3535
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539592.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539592.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGTA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAGACC 
GATGCAGTCT TACAGATGCA GATGGCTATT GATCAGGCCC ACAGGCAGAA TGTCTCCTCT 
CTTTTTCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAACATAGA TTACAAGAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTGGATG CAGGAGGGGT GCGCAAAGAA 
TTTTTCTTGC TCATCATGAG GGAATTATTG GATCCTAAAT ACGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTCCAT 
TTGATTGGTG TTATCTGTGG CTTAGCAATT TATAATTGTA CCATTGTGGA CCTCCATTTT 
CCTTTGGCTT TATATAAGAA ACTACTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAAGAA 
CTAATGCCTG ATGTTGGGAG AAGCATGCAA CAGTTACTGG ATTATCCAGA AGATGACATA 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAAGAGTTT 
GTCGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTATT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAAGTCCTTC TGCTCTTTCA GCCTAATGAA 
CTACAAGCAA TGGTCATTGG AAATACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAATATTG GGCAGAACAT CCTACGATAA AAATTTTTTG GGAAGTATTT 
CACGAATTAC CATTGGAAAA GAAGAAACAG TTTCTGTTAT TTTTGACAGG TAGTGATCGC 
ATTCCTATTC TTGGTATGAA GAGTCTGAAA CTAGTCATCC AGTCCACAGG AGGTGGTGAG 
GAGTATCTCC CAGTTTCCCA TACTTGTTTT AATCTTCTGG ATCTTCCAAA ATATACAGAA 
AAAGAAACTC TACGCTCTAA ACTGATCCAA GCTATTGATC ACAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539593.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537895.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu66793
Clone ID Related Accession (Same CDS sequence)	XM_011539593.3 , XM_011539593.2
Accession Version	XM_011539593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGTACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG CAGTCTTACA GATGCAGATG 
GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT TTCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT TCTTGCTCAT CATGAGGGAA 
TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTCCATTTGA TTGGTGTTAT CTGTGGCTTA 
GCAATTTATA ATTGTACCAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTA 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAAGAACTAA TGCCTGATGT TGGGAGAAGC 
ATGCAACAGT TACTGGATTA TCCAGAAGAT GACATAGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAA GAGTTTGTCG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAAG TCCTTCTGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT CATTGGAAAT 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA ATATTGGGCA 
GAACATCCTA CGATAAAAAT TTTTTGGGAA GTATTTCACG AATTACCATT GGAAAAGAAG 
AAACAGTTTC TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT 
CTGAAACTAG TCATCCAGTC CACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACT 
TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGAAAAAG AAACTCTACG CTCTAAACTG 
ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537895.1
CDS	290..3511
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539593.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539593.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGTACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG CAGTCTTACA GATGCAGATG 
GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT TTCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT TCTTGCTCAT CATGAGGGAA 
TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTCCATTTGA TTGGTGTTAT CTGTGGCTTA 
GCAATTTATA ATTGTACCAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTA 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAAGAACTAA TGCCTGATGT TGGGAGAAGC 
ATGCAACAGT TACTGGATTA TCCAGAAGAT GACATAGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAA GAGTTTGTCG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAAG TCCTTCTGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT CATTGGAAAT 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA ATATTGGGCA 
GAACATCCTA CGATAAAAAT TTTTTGGGAA GTATTTCACG AATTACCATT GGAAAAGAAG 
AAACAGTTTC TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT 
CTGAAACTAG TCATCCAGTC CACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACT 
TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGAAAAAG AAACTCTACG CTCTAAACTG 
ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539593.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537895.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66793
Clone ID Related Accession (Same CDS sequence)	XM_011539593.3 , XM_011539593.2
Accession Version	XM_011539593.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGTACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG CAGTCTTACA GATGCAGATG 
GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT TTCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT TCTTGCTCAT CATGAGGGAA 
TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTCCATTTGA TTGGTGTTAT CTGTGGCTTA 
GCAATTTATA ATTGTACCAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTA 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAAGAACTAA TGCCTGATGT TGGGAGAAGC 
ATGCAACAGT TACTGGATTA TCCAGAAGAT GACATAGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAA GAGTTTGTCG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAAG TCCTTCTGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT CATTGGAAAT 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA ATATTGGGCA 
GAACATCCTA CGATAAAAAT TTTTTGGGAA GTATTTCACG AATTACCATT GGAAAAGAAG 
AAACAGTTTC TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT 
CTGAAACTAG TCATCCAGTC CACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACT 
TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGAAAAAG AAACTCTACG CTCTAAACTG 
ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537895.1
CDS	314..3535
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539593.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539593.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCCTTTGGG CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTC TTTATAAATA AAAGGGTCCG AGATGTAGGA 
TGTGGACTCA GACATACTGT GTTTGTTCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACGTT AGCGCTAAAT 
GACAAAGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAGCTTGG CCTGGTAGGA 
TCAGAGGAAT GCATCAGAGT ACCCAGCTGC TTCCCGAAAA TCATGTGTGT CGACTCTCTG 
GTCAGAATTT GCTCCGGACT CTCTTATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATTG TACAGGTTGC TTGTGGTTAC TATCATTCAC TTGCACTTTC TAAAGCAAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAATTGG GTTTAGGTAC TGACTGTAAA 
AAGCAAACTT CACCGCAGCT GCTTAAGTCT TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATCTTTGG ATGGGGACGC 
AACAAGTTTG GTCAGCTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GATCTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCTGCT 
CTAACCAAGG AAGGTGGAGT GTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGCCAT 
AATTCTACCA GTCATGAAAT AAACCCAAGG AAAGTTTTTG AACTTATGGG AAGCATTGTC 
ACTGAGATTG CTTGTGGACG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TACTCTTTTG GGCTTGGTGG TAATGGGCAG CTGGGAACCG GTTCAACAAG CAACAGGAAA 
AGCCCCTTTA CTGTAAAAGG AAATTGGTAC CCCTATAATG GGCAGTGTCT ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTAAAAAGA ATTTTCTCAG GGGGAGATCA AAGCTTTTCA 
CATTACTCTA GTCCCCAGAA CTGTGGGCCA CCAGATGACT TCAGATGTCC CAATCCGACA 
AAGCAGATCT GGACAGTGAA TGAAGCTCTA ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA 
AGGTTTCCTG TGGAGATAGC CAATGAGATA GATGGAACGT TTTCTTCCTC TGGTTGCCTA 
AATGGAAGTT TTTTAGCTGT TAGCAATGAT GATCACTATA GAACAGGTAC CAGATTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCG 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGC 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTGAACCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATAG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGTATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAC TACATAGGGT AAATGAGAAA ATGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGAAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGTACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG CAGTCTTACA GATGCAGATG 
GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT TTCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT TCTTGCTCAT CATGAGGGAA 
TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTCCATTTGA TTGGTGTTAT CTGTGGCTTA 
GCAATTTATA ATTGTACCAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTA 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAAGAACTAA TGCCTGATGT TGGGAGAAGC 
ATGCAACAGT TACTGGATTA TCCAGAAGAT GACATAGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAA GAGTTTGTCG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAAG TCCTTCTGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT CATTGGAAAT 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA ATATTGGGCA 
GAACATCCTA CGATAAAAAT TTTTTGGGAA GTATTTCACG AATTACCATT GGAAAAGAAG 
AAACAGTTTC TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT 
CTGAAACTAG TCATCCAGTC CACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACT 
TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGAAAAAG AAACTCTACG CTCTAAACTG 
ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539594.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537896.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

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CloneID	OHu66794
Clone ID Related Accession (Same CDS sequence)	XM_011539594.2 , XM_011539595.2
Accession Version	XM_011539594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGCTGC 
TTCCCGAAAA TCATGTGTGT CGACTCTCTG GTCAGAATTT GCTCCGGACT CTCTTATGGC 
AGAATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATTG TACAGGTTGC TTGTGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCAAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAATTGG GTTTAGGTAC TGACTGTAAA AAGCAAACTT CACCGCAGCT GCTTAAGTCT 
TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATCTTTGG ATGGGGACGC AACAAGTTTG GTCAGCTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GATCTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCTGCT CTAACCAAGG AAGGTGGAGT GTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGCCAT AATTCTACCA GTCATGAAAT AAACCCAAGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTC ACTGAGATTG CTTGTGGACG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TACTCTTTTG GGCTTGGTGG TAATGGGCAG 
CTGGGAACCG GTTCAACAAG CAACAGGAAA AGCCCCTTTA CTGTAAAAGG AAATTGGTAC 
CCCTATAATG GGCAGTGTCT ACCAGATATT GATTCTGAAG AATATTTCTG TGTAAAAAGA 
ATTTTCTCAG GGGGAGATCA AAGCTTTTCA CATTACTCTA GTCCCCAGAA CTGTGGGCCA 
CCAGATGACT TCAGATGTCC CAATCCGACA AAGCAGATCT GGACAGTGAA TGAAGCTCTA 
ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA AGGTTTCCTG TGGAGATAGC CAATGAGATA 
GATGGAACGT TTTCTTCCTC TGGTTGCCTA AATGGAAGTT TTTTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGTAC CAGATTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCG CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGC TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTGAACCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATAG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGTATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAC TACATAGGGT AAATGAGAAA 
ATGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGAAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGTA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAGACC GATGCAGTCT TACAGATGCA GATGGCTATT 
GATCAGGCCC ACAGGCAGAA TGTCTCCTCT CTTTTTCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAACATAGA TTACAAGAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTGGATG CAGGAGGGGT GCGCAAAGAA TTTTTCTTGC TCATCATGAG GGAATTATTG 
GATCCTAAAT ACGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTCCAT TTGATTGGTG TTATCTGTGG CTTAGCAATT 
TATAATTGTA CCATTGTGGA CCTCCATTTT CCTTTGGCTT TATATAAGAA ACTACTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAAGAA CTAATGCCTG ATGTTGGGAG AAGCATGCAA 
CAGTTACTGG ATTATCCAGA AGATGACATA GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAAGAGTTT GTCGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTATT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAAGTCCTTC TGCTCTTTCA GCCTAATGAA CTACAAGCAA TGGTCATTGG AAATACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAATATTG GGCAGAACAT 
CCTACGATAA AAATTTTTTG GGAAGTATTT CACGAATTAC CATTGGAAAA GAAGAAACAG 
TTTCTGTTAT TTTTGACAGG TAGTGATCGC ATTCCTATTC TTGGTATGAA GAGTCTGAAA 
CTAGTCATCC AGTCCACAGG AGGTGGTGAG GAGTATCTCC CAGTTTCCCA TACTTGTTTT 
AATCTTCTGG ATCTTCCAAA ATATACAGAA AAAGAAACTC TACGCTCTAA ACTGATCCAA 
GCTATTGATC ACAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537896.1
CDS	610..3645
Translation	MAAHVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539594.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539594.2

ORF Insert Sequence:

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGCTGC 
TTCCCGAAAA TCATGTGTGT CGACTCTCTG GTCAGAATTT GCTCCGGACT CTCTTATGGC 
AGAATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATTG TACAGGTTGC TTGTGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCAAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAATTGG GTTTAGGTAC TGACTGTAAA AAGCAAACTT CACCGCAGCT GCTTAAGTCT 
TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATCTTTGG ATGGGGACGC AACAAGTTTG GTCAGCTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GATCTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCTGCT CTAACCAAGG AAGGTGGAGT GTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGCCAT AATTCTACCA GTCATGAAAT AAACCCAAGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTC ACTGAGATTG CTTGTGGACG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TACTCTTTTG GGCTTGGTGG TAATGGGCAG 
CTGGGAACCG GTTCAACAAG CAACAGGAAA AGCCCCTTTA CTGTAAAAGG AAATTGGTAC 
CCCTATAATG GGCAGTGTCT ACCAGATATT GATTCTGAAG AATATTTCTG TGTAAAAAGA 
ATTTTCTCAG GGGGAGATCA AAGCTTTTCA CATTACTCTA GTCCCCAGAA CTGTGGGCCA 
CCAGATGACT TCAGATGTCC CAATCCGACA AAGCAGATCT GGACAGTGAA TGAAGCTCTA 
ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA AGGTTTCCTG TGGAGATAGC CAATGAGATA 
GATGGAACGT TTTCTTCCTC TGGTTGCCTA AATGGAAGTT TTTTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGTAC CAGATTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCG CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGC TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTGAACCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATAG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGTATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAC TACATAGGGT AAATGAGAAA 
ATGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGAAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGTA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAGACC GATGCAGTCT TACAGATGCA GATGGCTATT 
GATCAGGCCC ACAGGCAGAA TGTCTCCTCT CTTTTTCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAACATAGA TTACAAGAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTGGATG CAGGAGGGGT GCGCAAAGAA TTTTTCTTGC TCATCATGAG GGAATTATTG 
GATCCTAAAT ACGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTCCAT TTGATTGGTG TTATCTGTGG CTTAGCAATT 
TATAATTGTA CCATTGTGGA CCTCCATTTT CCTTTGGCTT TATATAAGAA ACTACTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAAGAA CTAATGCCTG ATGTTGGGAG AAGCATGCAA 
CAGTTACTGG ATTATCCAGA AGATGACATA GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAAGAGTTT GTCGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTATT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAAGTCCTTC TGCTCTTTCA GCCTAATGAA CTACAAGCAA TGGTCATTGG AAATACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAATATTG GGCAGAACAT 
CCTACGATAA AAATTTTTTG GGAAGTATTT CACGAATTAC CATTGGAAAA GAAGAAACAG 
TTTCTGTTAT TTTTGACAGG TAGTGATCGC ATTCCTATTC TTGGTATGAA GAGTCTGAAA 
CTAGTCATCC AGTCCACAGG AGGTGGTGAG GAGTATCTCC CAGTTTCCCA TACTTGTTTT 
AATCTTCTGG ATCTTCCAAA ATATACAGAA AAAGAAACTC TACGCTCTAA ACTGATCCAA 
GCTATTGATC ACAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_011539595.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537897.1 Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

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CloneID	OHu66794
Clone ID Related Accession (Same CDS sequence)	XM_011539594.2 , XM_011539595.2
Accession Version	XM_011539595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGCTGC 
TTCCCGAAAA TCATGTGTGT CGACTCTCTG GTCAGAATTT GCTCCGGACT CTCTTATGGC 
AGAATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATTG TACAGGTTGC TTGTGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCAAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAATTGG GTTTAGGTAC TGACTGTAAA AAGCAAACTT CACCGCAGCT GCTTAAGTCT 
TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATCTTTGG ATGGGGACGC AACAAGTTTG GTCAGCTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GATCTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCTGCT CTAACCAAGG AAGGTGGAGT GTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGCCAT AATTCTACCA GTCATGAAAT AAACCCAAGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTC ACTGAGATTG CTTGTGGACG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TACTCTTTTG GGCTTGGTGG TAATGGGCAG 
CTGGGAACCG GTTCAACAAG CAACAGGAAA AGCCCCTTTA CTGTAAAAGG AAATTGGTAC 
CCCTATAATG GGCAGTGTCT ACCAGATATT GATTCTGAAG AATATTTCTG TGTAAAAAGA 
ATTTTCTCAG GGGGAGATCA AAGCTTTTCA CATTACTCTA GTCCCCAGAA CTGTGGGCCA 
CCAGATGACT TCAGATGTCC CAATCCGACA AAGCAGATCT GGACAGTGAA TGAAGCTCTA 
ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA AGGTTTCCTG TGGAGATAGC CAATGAGATA 
GATGGAACGT TTTCTTCCTC TGGTTGCCTA AATGGAAGTT TTTTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGTAC CAGATTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCG CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGC TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTGAACCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATAG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGTATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAC TACATAGGGT AAATGAGAAA 
ATGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGAAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGTA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAGACC GATGCAGTCT TACAGATGCA GATGGCTATT 
GATCAGGCCC ACAGGCAGAA TGTCTCCTCT CTTTTTCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAACATAGA TTACAAGAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTGGATG CAGGAGGGGT GCGCAAAGAA TTTTTCTTGC TCATCATGAG GGAATTATTG 
GATCCTAAAT ACGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTCCAT TTGATTGGTG TTATCTGTGG CTTAGCAATT 
TATAATTGTA CCATTGTGGA CCTCCATTTT CCTTTGGCTT TATATAAGAA ACTACTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAAGAA CTAATGCCTG ATGTTGGGAG AAGCATGCAA 
CAGTTACTGG ATTATCCAGA AGATGACATA GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAAGAGTTT GTCGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTATT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAAGTCCTTC TGCTCTTTCA GCCTAATGAA CTACAAGCAA TGGTCATTGG AAATACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAATATTG GGCAGAACAT 
CCTACGATAA AAATTTTTTG GGAAGTATTT CACGAATTAC CATTGGAAAA GAAGAAACAG 
TTTCTGTTAT TTTTGACAGG TAGTGATCGC ATTCCTATTC TTGGTATGAA GAGTCTGAAA 
CTAGTCATCC AGTCCACAGG AGGTGGTGAG GAGTATCTCC CAGTTTCCCA TACTTGTTTT 
AATCTTCTGG ATCTTCCAAA ATATACAGAA AAAGAAACTC TACGCTCTAA ACTGATCCAA 
GCTATTGATC ACAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537897.1
CDS	660..3695
Translation	MAAHVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPSCFPKIMCVDSLVRICSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_011539595.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539595.2

ORF Insert Sequence:

ATGGCAGCAC ACGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
TGTGGAGAAG CTCATACGTT AGCGCTAAAT GACAAAGGCC AGGTGTATGC TTGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGGTAGGA TCAGAGGAAT GCATCAGAGT ACCCAGCTGC 
TTCCCGAAAA TCATGTGTGT CGACTCTCTG GTCAGAATTT GCTCCGGACT CTCTTATGGC 
AGAATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATTG TACAGGTTGC TTGTGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCAAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAATTGG GTTTAGGTAC TGACTGTAAA AAGCAAACTT CACCGCAGCT GCTTAAGTCT 
TTGCTTGGAA TCCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATCTTTGG ATGGGGACGC AACAAGTTTG GTCAGCTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GATCTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCTGCT CTAACCAAGG AAGGTGGAGT GTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGCCAT AATTCTACCA GTCATGAAAT AAACCCAAGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTC ACTGAGATTG CTTGTGGACG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TACTCTTTTG GGCTTGGTGG TAATGGGCAG 
CTGGGAACCG GTTCAACAAG CAACAGGAAA AGCCCCTTTA CTGTAAAAGG AAATTGGTAC 
CCCTATAATG GGCAGTGTCT ACCAGATATT GATTCTGAAG AATATTTCTG TGTAAAAAGA 
ATTTTCTCAG GGGGAGATCA AAGCTTTTCA CATTACTCTA GTCCCCAGAA CTGTGGGCCA 
CCAGATGACT TCAGATGTCC CAATCCGACA AAGCAGATCT GGACAGTGAA TGAAGCTCTA 
ATTCAGAAAT GGCTGAGCTA TCCTTCTGGA AGGTTTCCTG TGGAGATAGC CAATGAGATA 
GATGGAACGT TTTCTTCCTC TGGTTGCCTA AATGGAAGTT TTTTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGTAC CAGATTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCG CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGC TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTGAACCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATAG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGTATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAC TACATAGGGT AAATGAGAAA 
ATGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGAAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGTA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAGACC GATGCAGTCT TACAGATGCA GATGGCTATT 
GATCAGGCCC ACAGGCAGAA TGTCTCCTCT CTTTTTCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTGGT GCGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAACATAGA TTACAAGAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTGGATG CAGGAGGGGT GCGCAAAGAA TTTTTCTTGC TCATCATGAG GGAATTATTG 
GATCCTAAAT ACGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTCCAT TTGATTGGTG TTATCTGTGG CTTAGCAATT 
TATAATTGTA CCATTGTGGA CCTCCATTTT CCTTTGGCTT TATATAAGAA ACTACTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAAGAA CTAATGCCTG ATGTTGGGAG AAGCATGCAA 
CAGTTACTGG ATTATCCAGA AGATGACATA GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAAGAGTTT GTCGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTATT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAAGTCCTTC TGCTCTTTCA GCCTAATGAA CTACAAGCAA TGGTCATTGG AAATACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAATATTG GGCAGAACAT 
CCTACGATAA AAATTTTTTG GGAAGTATTT CACGAATTAC CATTGGAAAA GAAGAAACAG 
TTTCTGTTAT TTTTGACAGG TAGTGATCGC ATTCCTATTC TTGGTATGAA GAGTCTGAAA 
CTAGTCATCC AGTCCACAGG AGGTGGTGAG GAGTATCTCC CAGTTTCCCA TACTTGTTTT 
AATCTTCTGG ATCTTCCAAA ATATACAGAA AAAGAAACTC TACGCTCTAA ACTGATCCAA 
GCTATTGATC ACAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIRT1/HERC4 Locus Associated With Bisphosphonate-Induced Osteonecrosis of the Jaw: An Exome-Wide Association Analysis.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research33(1)91-98(2018 Jan)
Yang G,Hamadeh IS,Katz J,Riva A,Lakatos P,Balla B,Kosa J,Vaszilko M,Pelliccioni GA,Davis N,Langaee TY,Moreb JS,Gong Y

HERC4 Is Overexpressed in Hepatocellular Carcinoma and Contributes to the Proliferation and Migration of Hepatocellular Carcinoma Cells.
DNA and cell biology36(6)490-500(2017 Jun)
Zheng Y,Li J,Pan C,Zhou G,Zhuge L,Jin L,Fang P

Expression of HERC4 in lung cancer and its correlation with clinicopathological parameters.
Asian Pacific journal of cancer prevention : APJCP16(2)513-7(2015)
Zeng WL,Chen YW,Zhou H,Zhou JY,Wei M,Shi R

Disruption of the ubiquitin ligase HERC4 causes defects in spermatozoon maturation and impaired fertility.
Developmental biology312(2)501-8(2007 Dec)
Rodriguez CI,Stewart CL

A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
American journal of human genetics78(1)78-88(2006 Jan)
Grupe A,Li Y,Rowland C,Nowotny P,Hinrichs AL,Smemo S,Kauwe JS,Maxwell TJ,Cherny S,Doil L,Tacey K,van Luchene R,Myers A,Wavrant-De Vri?ze F,Kaleem M,Hollingworth P,Jehu L,Foy C,Archer N,Hamilton G,Holmans P,Morris CM,Catanese J,Sninsky J,White TJ,Powell J,Hardy J,O'Donovan M,Lovestone S,Jones L,Morris JC,Thal L,Owen M,Williams J,Goate A

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HERC4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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