MAGI3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGI3
Entrez Gene ID	260425
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms	MAGI-3,dJ730K3.2
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 3 Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 membrane-associated guanylate kinase inverted 3 membrane-associated guanylate kinase-related 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.2
Disorder MIM:	615943

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142782.2	NP_001136254.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
NM_152900.2	NP_690864.2	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 2
NM_001142782.1	NP_001136254.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
NM_152900.3	NP_690864.2	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 2
XM_011541208.2	XP_011539510.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
XM_017000974.1	XP_016856463.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X2
XM_005270737.3	XP_005270794.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X3
XM_017000975.1	XP_016856464.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Magi3	NP_620784.2
Canis lupus familiaris (dog)	MAGI3	XP_005630899.1
Gallus gallus (chicken)	MAGI3	NP_001012715.1
	LOC709341	XP_001097865.1
Bos taurus (cattle)	MAGI3	NP_001179675.1
Danio rerio (zebrafish)	LOC564220	XP_692662.4
Xenopus tropicalis (tropical clawed frog)	magi3	NP_001090712.1
Homo sapiens (human)	MAGI3	NP_001136254.1
Mus musculus (house mouse)	Magi3	NP_001152826.1
Danio rerio (zebrafish)	magi3	XP_690190.6

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variation and expression levels of tight junction genes identifies association between MAGI3 and inflammatory bowel disease.
Nor?n E, Almer S, S?derman J
BMC gastroenterology17(1)68(2017 May)

Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer.
Ni TK, Kuperwasser C
eLife5(2016 05)

MAGI3 negatively regulates Wnt/?-catenin signaling and suppresses malignant phenotypes of glioma cells.
Ma Q, Yang Y, Feng D, Zheng S, Meng R, Fa P, Zhao C, Liu H, Song R, Tao T, Yang L, Dai J, Wang S, Jiang WG, He J
Oncotarget6(34)35851-65(2015 Nov)

MAGI3 Suppresses Glioma Cell Proliferation via Upregulation of PTEN Expression.
Ma Q, Zhang Y, Meng R, Xie KM, Xiong Y, Lin S, He ZL, Tao T, Yang Y, Zhao JZ, He JQ
Biomedical and environmental sciences : BES28(7)502-9(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The altered mRNA isoform, called MAGI3(pPA), produces a truncated protein that acts in a dominant-negative manner to prevent full-length MAGI3 from interacting with the YAP oncoprotein, thereby relieving YAP inhibition and promoting malignant transformation of human mammary epithelial cells.
Title: Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer.

Results identify MAGI3 as a novel tumor suppressor and provide insight into the pathogenesis of glioma.
Title: MAGI3 negatively regulates Wnt/?-catenin signaling and suppresses malignant phenotypes of glioma cells.

Expression levels of MAGI3 and PTEN were significantly downregulated in gliomas.
Title: MAGI3 Suppresses Glioma Cell Proliferation via Upregulation of PTEN Expression.

MAGI-3 competes with NHERF-2 to negatively regulate LPA2 receptor signaling in colon cancer cells.
Title: MAGI-3 competes with NHERF-2 to negatively regulate LPA2 receptor signaling in colon cancer cells.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following MAGI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu87217	XM_017000975.1 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87216	XM_017000974.1 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu13370	NM_152900.3 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu13370	XM_005270737.3 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu78486	XM_011541208.2 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu13683	NM_001142782.2 Latest version!	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu13683	NM_001142782.1	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu13370	NM_152900.2	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGI3 ( XM_017000975.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856464.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu87217
Clone ID Related Accession (Same CDS sequence)	XM_017000975.1
Accession Version	XM_017000975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAT CACACTTCAC AAGAGATCCA TCCCAGCTTA AAGGTGTCCT TGTTCGAGCA 
TCACTGAAAA AAAGCACAAT GGGATTTGGT TTTACTATTA TTGGTGGAGA TAGACCTGAT 
GAGTTCCTAC AAGTGAAAAA TGTGCTGAAA GATGGTCCCG CAGCTCAGGA TGGGAAAATT 
GCACCAGGCG ATGTTATTGT AGACATCAAT GGCAACTGTG TCCTCGGTCA CACTCATGCA 
GATGTTGTCC AGATGTTTCA ATTGGTACCT GTCAATCAGT ATGTAAACCT CACTTTATGT 
CGTGGTTATC CACTTCCTGA TGACAGTGAA GATCCTGTTG TGGACATTGT TGCTGCTACC 
CCTGTCATCA ATGGACAGTC ATTAACCAAG GGAGAGACTT GCATGAATCC TCAGGATTTT 
AAGCCAGGAG CAATGGTTCT GGAGCAGAAT GGAAAATCGG GACACACTTT GACTGGTGAT 
GGTCTCAATG GACCATCAGA TGCAAGTGAG CAGAGAGTAT CCATGGCATC GTCAGGCAGC 
TCCCAGCCTG AACTAGTGAC TATCCCTTTG ATTAAGGGCC CTAAAGGGTT TGGGTTTGCA 
ATTGCTGACA GCCCTACTGG ACAGAAGGTG AAAATGATAC TGGATAGTCA GTGGTGTCAA 
GGCCTTCAGA AAGGAGATAT AATTAAGGAA ATATACCATC AAAATGTGCA GAATTTAACA 
CATCTCCAAG TGGTAGAGGT GCTAAAGCAG TTTCCAGTAG GTGCTGATGT ACCATTGCTT 
ATCTTAAGAG GAGGTCCTCC TTCACCAACC AAAACTGCCA AAATGAAAAC AGATAAAAAG 
GAAAATGCAG GAAGTTTGGA GGCCATAAAT GAGCCTATTC CTCAGCCTAT GCCTTTTCCA 
CCGAGCATTA TCAGGTCAGG ATCCCCAAAA TTGGATCCTT CTGAGGTCTA CCTGAAATCT 
AAGACTTTAT ATGAAGATAA ACCACCAAAC ACCAAAGATT TGGATGTTTT TCTTCGAAAA 
CAAGAGTCAG GGTTTGGCTT CAGGGTGCTA GGAGGAGATG GACCTGACCA GTCTATATAT 
ATTGGGGCTA TTATTCCCCT GGGAGCAGCT GAGAAAGATG GTCGGCTCCG CGCAGCTGAT 
GAACTAATGT GCATTGATGG AATTCCTGTT AAAGGGAAAT CACACAAACA AGTCTTGGAC 
CTCATGACAA CTGCTGCTCG AAATGGCCAT GTGTTACTAA CTGTCAGACG GAAGATCTTC 
TATGGAGAAA AACAACCCGA GGACGACAGC TCTCAGGCCT TCATTTCAAC ACAGAATGGA 
TCTCCCCGCC TGAACCGGGC AGAGGTCCCA GCCAGGCCTG CACCCCAGGA GCCCTATGAT 
GTTGTCTTGC AACGAAAAGA AAATGAAGGA TTTGGCTTTG TCATCCTCAC CTCCAAAAAC 
AAACCACCTC CAGGAGTTAT TCCTCATAAA ATTGGCCGAG TCATAGAAGG AAGTCCGGCT 
GACCGCTGTG GAAAACTGAA AGTTGGAGAT CATATCTCTG CAGTGAATGG GCAGTCCATT 
GTTGAACTGT CTCATGATAA CATTGTTCAG CTGATCAAAG ATGCTGGTGT CACCGTCACA 
CTAACGGTCA TTGCTGAAGA AGAGCATCAT GGTCCACCAT CAGGAACAAA CTCAGCCAGG 
CAAAGCCCAG CCCTGCAGCA CAGGCCCATG GGACAGTCAC AGGCCAACCA CATACCTGGG 
GACAGAAGTG CCCTAGAAGG TGAAATTGGA AAAGATGTCT CCACTTCTTA CAGACATTCT 
TGGTCAGACC ACAAGCACCT TGCACAGCCT GACACCGCAG TAATTTCAGT TGTAGGCAGT 
CGGCACAATC AGAACCTTGG TTGTTATCCA GTAGAGCTGG AGAGAGGCCC CCGGGGCTTT 
GGATTCAGCC TCCGAGGGGG GAAGGAGTAC AACATGGGGC TGTTCATCCT TCGTCTTGCT 
GAAGATGGTC CTGCCATCAA AGATGGCAGA ATTCATGTTG GTGACCAGAT TGTTGAAATC 
AATGGGGAAC CTACACAAGG AATCACACAT ACTCGAGCAA TTGAGCTCAT TCAGGCTGGT 
GGAAATAAAG TTCTTCTTCT TTTGAGGCCA GGAACTGGCT TGATACCTGA CCATGTCCTA 
GGCTTTTTCT CTTCCCTCTT CAGGTTTGGC TCCTTCCGGT CTGTGCTCCT ACGTGAAACC 
CGAGCAACAT TAAGGCTTTC AGGGCTTTTC TTGGTCTTTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856464.1
CDS	327..2591
Translation	MEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHVLGFFSSLFRFGSFRSVLLRETRATLRLSGLFLVFP

Target ORF information:

RefSeq Version	XM_017000975.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000975.1

ORF Insert Sequence:

ATGGAAAAAT CACACTTCAC AAGAGATCCA TCCCAGCTTA AAGGTGTCCT TGTTCGAGCA 
TCACTGAAAA AAAGCACAAT GGGATTTGGT TTTACTATTA TTGGTGGAGA TAGACCTGAT 
GAGTTCCTAC AAGTGAAAAA TGTGCTGAAA GATGGTCCCG CAGCTCAGGA TGGGAAAATT 
GCACCAGGCG ATGTTATTGT AGACATCAAT GGCAACTGTG TCCTCGGTCA CACTCATGCA 
GATGTTGTCC AGATGTTTCA ATTGGTACCT GTCAATCAGT ATGTAAACCT CACTTTATGT 
CGTGGTTATC CACTTCCTGA TGACAGTGAA GATCCTGTTG TGGACATTGT TGCTGCTACC 
CCTGTCATCA ATGGACAGTC ATTAACCAAG GGAGAGACTT GCATGAATCC TCAGGATTTT 
AAGCCAGGAG CAATGGTTCT GGAGCAGAAT GGAAAATCGG GACACACTTT GACTGGTGAT 
GGTCTCAATG GACCATCAGA TGCAAGTGAG CAGAGAGTAT CCATGGCATC GTCAGGCAGC 
TCCCAGCCTG AACTAGTGAC TATCCCTTTG ATTAAGGGCC CTAAAGGGTT TGGGTTTGCA 
ATTGCTGACA GCCCTACTGG ACAGAAGGTG AAAATGATAC TGGATAGTCA GTGGTGTCAA 
GGCCTTCAGA AAGGAGATAT AATTAAGGAA ATATACCATC AAAATGTGCA GAATTTAACA 
CATCTCCAAG TGGTAGAGGT GCTAAAGCAG TTTCCAGTAG GTGCTGATGT ACCATTGCTT 
ATCTTAAGAG GAGGTCCTCC TTCACCAACC AAAACTGCCA AAATGAAAAC AGATAAAAAG 
GAAAATGCAG GAAGTTTGGA GGCCATAAAT GAGCCTATTC CTCAGCCTAT GCCTTTTCCA 
CCGAGCATTA TCAGGTCAGG ATCCCCAAAA TTGGATCCTT CTGAGGTCTA CCTGAAATCT 
AAGACTTTAT ATGAAGATAA ACCACCAAAC ACCAAAGATT TGGATGTTTT TCTTCGAAAA 
CAAGAGTCAG GGTTTGGCTT CAGGGTGCTA GGAGGAGATG GACCTGACCA GTCTATATAT 
ATTGGGGCTA TTATTCCCCT GGGAGCAGCT GAGAAAGATG GTCGGCTCCG CGCAGCTGAT 
GAACTAATGT GCATTGATGG AATTCCTGTT AAAGGGAAAT CACACAAACA AGTCTTGGAC 
CTCATGACAA CTGCTGCTCG AAATGGCCAT GTGTTACTAA CTGTCAGACG GAAGATCTTC 
TATGGAGAAA AACAACCCGA GGACGACAGC TCTCAGGCCT TCATTTCAAC ACAGAATGGA 
TCTCCCCGCC TGAACCGGGC AGAGGTCCCA GCCAGGCCTG CACCCCAGGA GCCCTATGAT 
GTTGTCTTGC AACGAAAAGA AAATGAAGGA TTTGGCTTTG TCATCCTCAC CTCCAAAAAC 
AAACCACCTC CAGGAGTTAT TCCTCATAAA ATTGGCCGAG TCATAGAAGG AAGTCCGGCT 
GACCGCTGTG GAAAACTGAA AGTTGGAGAT CATATCTCTG CAGTGAATGG GCAGTCCATT 
GTTGAACTGT CTCATGATAA CATTGTTCAG CTGATCAAAG ATGCTGGTGT CACCGTCACA 
CTAACGGTCA TTGCTGAAGA AGAGCATCAT GGTCCACCAT CAGGAACAAA CTCAGCCAGG 
CAAAGCCCAG CCCTGCAGCA CAGGCCCATG GGACAGTCAC AGGCCAACCA CATACCTGGG 
GACAGAAGTG CCCTAGAAGG TGAAATTGGA AAAGATGTCT CCACTTCTTA CAGACATTCT 
TGGTCAGACC ACAAGCACCT TGCACAGCCT GACACCGCAG TAATTTCAGT TGTAGGCAGT 
CGGCACAATC AGAACCTTGG TTGTTATCCA GTAGAGCTGG AGAGAGGCCC CCGGGGCTTT 
GGATTCAGCC TCCGAGGGGG GAAGGAGTAC AACATGGGGC TGTTCATCCT TCGTCTTGCT 
GAAGATGGTC CTGCCATCAA AGATGGCAGA ATTCATGTTG GTGACCAGAT TGTTGAAATC 
AATGGGGAAC CTACACAAGG AATCACACAT ACTCGAGCAA TTGAGCTCAT TCAGGCTGGT 
GGAAATAAAG TTCTTCTTCT TTTGAGGCCA GGAACTGGCT TGATACCTGA CCATGTCCTA 
GGCTTTTTCT CTTCCCTCTT CAGGTTTGGC TCCTTCCGGT CTGTGCTCCT ACGTGAAACC 
CGAGCAACAT TAAGGCTTTC AGGGCTTTTC TTGGTCTTTC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( XM_017000974.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856463.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu87216
Clone ID Related Accession (Same CDS sequence)	XM_017000974.1
Accession Version	XM_017000974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGTC CTAGGCTTTT TCTCTTCCCT CTTCAGGTTT 
GGCTCCTTCC GGTCTGTGCT CCTACGTGAA ACCCGAGCAA CATTAAGGCT TTCAGGGCTT 
TTCTTGGTCT TTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856463.1
CDS	561..3998
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHVLGFFSSLFRFGSFRSVLLRETRATLRLSGLFLVFP

Target ORF information:

RefSeq Version	XM_017000974.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000974.1

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGTC CTAGGCTTTT TCTCTTCCCT CTTCAGGTTT 
GGCTCCTTCC GGTCTGTGCT CCTACGTGAA ACCCGAGCAA CATTAAGGCT TTCAGGGCTT 
TTCTTGGTCT TTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( NM_152900.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_690864.2 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.

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CloneID	OHu13370
Clone ID Related Accession (Same CDS sequence)	NM_152900.2 , NM_152900.3 , XM_005270737.3
Accession Version	NM_152900.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC130409.1 and AL133517.11. On Aug 15, 2007 this sequence version replaced NM_152900.1. Transcript Variant: This variant (2) uses an alternate splice site at the 5' end of the last coding exon, compared to variant 1, that results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC130409.1, AF213259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690864.2
CDS	182..3559
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSYVKPEQH

Target ORF information:

RefSeq Version	NM_152900.2
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152900.2

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( NM_152900.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_690864.2 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13370
Clone ID Related Accession (Same CDS sequence)	NM_152900.2 , NM_152900.3 , XM_005270737.3
Accession Version	NM_152900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL365225.13, BC130409.1 and AL133517.11. On May 31, 2019 this sequence version replaced NM_152900.2. Transcript Variant: This variant (2) uses an alternate splice site at the 5' end of the last coding exon, compared to variant 1, that results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC130409.1, AF213259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690864.2
CDS	520..3897
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSYVKPEQH

Target ORF information:

RefSeq Version	NM_152900.3
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152900.3

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( XM_005270737.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270794.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X3, mRNA.

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CloneID	OHu13370
Clone ID Related Accession (Same CDS sequence)	NM_152900.2 , NM_152900.3 , XM_005270737.3
Accession Version	XM_005270737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270794.1
CDS	182..3559
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSYVKPEQH

Target ORF information:

RefSeq Version	XM_005270737.3
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270737.3

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT TTGGCTCCTT CCGGTCTGTG CTCCTACGTG 
AAACCCGAGC AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( XM_011541208.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539510.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X1, mRNA.

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CloneID	OHu78486
Clone ID Related Accession (Same CDS sequence)	XM_011541208.2
Accession Version	XM_011541208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA TGGATAGCTC TCTTCCTGAA GAGGAAGAAG ATGAGGACAA GGAAGCTATT 
AATGGCAGTG GAAACGCAGA AAACAGAGAG AGGCATTCTG AGTCATCTGA CTGGATGAAG 
ACTGTTCCAA GTTACAACCA AACAAATAGC TCCATGGACT TTAGAAATTA TATGATGAGA 
GATGAGACTC TGGAACCACT GCCCAAAAAC TGGGAAATGG CCTACACTGA CACAGGGATG 
ATCTACTTCA TTGACCACAA TACCAAGACA ACCACCTGGT TGGATCCTCG TCTTTGTAAG 
AAAGCCAAAG CCCCTGAAGA CTGTGAAGAT GGAGAGCTTC CTTATGGCTG GGAGAAAATA 
GAGGACCCTC AGTATGGGAC ATACTATGTT GATCACCTTA ACCAGAAAAC CCAGTTTGAA 
AATCCAGTGG AGGAAGCCAA AAGGAAAAAG CAGTTAGGAC AGGTTGAAAT TGGGTCTTCA 
AAACCAGATA TGGAAAAATC ACACTTCACA AGAGATCCAT CCCAGCTTAA AGGTGTCCTT 
GTTCGAGCAT CACTGAAAAA AAGCACAATG GGATTTGGTT TTACTATTAT TGGTGGAGAT 
AGACCTGATG AGTTCCTACA AGTGAAAAAT GTGCTGAAAG ATGGTCCCGC AGCTCAGGAT 
GGGAAAATTG CACCAGGCGA TGTTATTGTA GACATCAATG GCAACTGTGT CCTCGGTCAC 
ACTCATGCAG ATGTTGTCCA GATGTTTCAA TTGGTACCTG TCAATCAGTA TGTAAACCTC 
ACTTTATGTC GTGGTTATCC ACTTCCTGAT GACAGTGAAG ATCCTGTTGT GGACATTGTT 
GCTGCTACCC CTGTCATCAA TGGACAGTCA TTAACCAAGG GAGAGACTTG CATGAATCCT 
CAGGATTTTA AGCCAGGAGC AATGGTTCTG GAGCAGAATG GAAAATCGGG ACACACTTTG 
ACTGGTGATG GTCTCAATGG ACCATCAGAT GCAAGTGAGC AGAGAGTATC CATGGCATCG 
TCAGGCAGCT CCCAGCCTGA ACTAGTGACT ATCCCTTTGA TTAAGGGCCC TAAAGGGTTT 
GGGTTTGCAA TTGCTGACAG CCCTACTGGA CAGAAGGTGA AAATGATACT GGATAGTCAG 
TGGTGTCAAG GCCTTCAGAA AGGAGATATA ATTAAGGAAA TATACCATCA AAATGTGCAG 
AATTTAACAC ATCTCCAAGT GGTAGAGGTG CTAAAGCAGT TTCCAGTAGG TGCTGATGTA 
CCATTGCTTA TCTTAAGAGG AGGTCCTCCT TCACCAACCA AAACTGCCAA AATGAAAACA 
GATAAAAAGG AAAATGCAGG AAGTTTGGAG GCCATAAATG AGCCTATTCC TCAGCCTATG 
CCTTTTCCAC CGAGCATTAT CAGGTCAGGA TCCCCAAAAT TGGATCCTTC TGAGGTCTAC 
CTGAAATCTA AGACTTTATA TGAAGATAAA CCACCAAACA CCAAAGATTT GGATGTTTTT 
CTTCGAAAAC AAGAGTCAGG GTTTGGCTTC AGGGTGCTAG GAGGAGATGG ACCTGACCAG 
TCTATATATA TTGGGGCTAT TATTCCCCTG GGAGCAGCTG AGAAAGATGG TCGGCTCCGC 
GCAGCTGATG AACTAATGTG CATTGATGGA ATTCCTGTTA AAGGGAAATC ACACAAACAA 
GTCTTGGACC TCATGACAAC TGCTGCTCGA AATGGCCATG TGTTACTAAC TGTCAGACGG 
AAGATCTTCT ATGGAGAAAA ACAACCCGAG GACGACAGCT CTCAGGCCTT CATTTCAACA 
CAGAATGGAT CTCCCCGCCT GAACCGGGCA GAGGTCCCAG CCAGGCCTGC ACCCCAGGAG 
CCCTATGATG TTGTCTTGCA ACGAAAAGAA AATGAAGGAT TTGGCTTTGT CATCCTCACC 
TCCAAAAACA AACCACCTCC AGGAGTTATT CCTCATAAAA TTGGCCGAGT CATAGAAGGA 
AGTCCGGCTG ACCGCTGTGG AAAACTGAAA GTTGGAGATC ATATCTCTGC AGTGAATGGG 
CAGTCCATTG TTGAACTGTC TCATGATAAC ATTGTTCAGC TGATCAAAGA TGCTGGTGTC 
ACCGTCACAC TAACGGTCAT TGCTGAAGAA GAGCATCATG GTCCACCATC AGGAACAAAC 
TCAGCCAGGC AAAGCCCAGC CCTGCAGCAC AGGCCCATGG GACAGTCACA GGCCAACCAC 
ATACCTGGGG ACAGAAGTGC CCTAGAAGGT GAAATTGGAA AAGATGTCTC CACTTCTTAC 
AGACATTCTT GGTCAGACCA CAAGCACCTT GCACAGCCTG ACACCGCAGT AATTTCAGTT 
GTAGGCAGTC GGCACAATCA GAACCTTGGT TGTTATCCAG TAGAGCTGGA GAGAGGCCCC 
CGGGGCTTTG GATTCAGCCT CCGAGGGGGG AAGGAGTACA ACATGGGGCT GTTCATCCTT 
CGTCTTGCTG AAGATGGTCC TGCCATCAAA GATGGCAGAA TTCATGTTGG TGACCAGATT 
GTTGAAATCA ATGGGGAACC TACACAAGGA ATCACACATA CTCGAGCAAT TGAGCTCATT 
CAGGCTGGTG GAAATAAAGT TCTTCTTCTT TTGAGGCCAG GAACTGGCTT GATACCTGAC 
CATGGTGATT GGGATATTAA TAATCCTTCG TCTTCAAATG TGATTTATGA TGAACAGTCA 
CCATTACCCC CATCTTCACA TTTTGCTTCC ATATTTGAAG AGTCTCACGT GCCAGTAATT 
GAAGAATCTT TGAGAGTTCA GATATGTGAA AAGGCAGAAG AATTAAAGGA CATTGTGCCT 
GAAAAGAAAA GCACTTTAAA TGAAAATCAG CCTGAGATAA AGCATCAGTC TCTTCTCCAG 
AAAAATGTGA GTAAGAGGGA TCCACCCAGC AGTCATGGGC ACAGTAACAA GAAAAATCTA 
TTAAAAGTAG AAAATGGTGT TACACGAAGA GGTAGATCGG TTAGTCCCAA AAAGCCAGCC 
AGTCAACATT CAGAGGAACA TTTGGATAAG ATTCCTAGTC CTCTAAAAAA TAACCCCAAA 
AGAAGACCCA GAGATCAATC CCTCAGCCCC AGCAAAGGGG AAAATAAAAG TTGTCAGGTC 
AGCACCAGGG CAGGCTCTGG ACAAGATCAG TGCAGAAAAA GCAGAGGTCG GTCGGCCAGC 
CCAAAAAAGC AGCAAAAAAT TGAAGGAAGC AAAGCTCCAT CAAATGCTGA GGCCAAATTA 
TTAGAGGGTA AGAGTCGAAG AATAGCAGGC TATACGGGCA GTAATGCTGA GCAGATCCCA 
GATGGGAAGG AAAAATCAGA CGTCATCAGG AAAGATGCAA AGCAGAATCA GTTGGAAAAA 
AGCAGAACAA GGTCTCCAGA GAAAAAAATC AAAAGAATGG TTGAGAAATC TCTTCCATCC 
AAAATGACTA ATAAGACTAC AAGTAAAGAA GTATCTGAAA ATGAAAAAGG AAAGAAAGTA 
ACCACAGGAG AAACAAGTTC TAGTAACGAT AAAATAGGAG AAAATGTCCA GCTATCAGAA 
AAGAGGCTGA AGCAAGAACC TGAAGAGAAG GTAGTTTCAA ACAAAACAGA AGATCACAAA 
GGGAAAGAAC TAGAGGCAGC TGACAAAAAC AAAGAGACTG GAAGGTTCAA ACCGGAAAGC 
AGTTCTCCAG TTAAGAAAAC ACTGATAACT CCAGGGCCCT GGAAGGTTCC AAGTGGAAAT 
AAAGTCACAG GCACTATTGG TATGGCTGAG AAACGGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539510.1
CDS	2817..6578
Translation	MERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASIFEESHVPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQSLLQKNVSKRDPPSSHGHSNKKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGENKSCQVSTRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYTGSNAEQIPDGKEKSDVIRKDAKQNQLEKSRTRSPEKKIKRMVEKSLPSKMTNKTTSKEVSENEKGKKVTTGETSSSNDKIGENVQLSEKRLKQEPEEKVVSNKTEDHKGKELEAADKNKETGRFKPESSSPVKKTLITPGPWKVPSGNKVTGTIGMAEKRQ

Target ORF information:

RefSeq Version	XM_011541208.2
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541208.2

ORF Insert Sequence:

ATGGAAAGAA TGGATAGCTC TCTTCCTGAA GAGGAAGAAG ATGAGGACAA GGAAGCTATT 
AATGGCAGTG GAAACGCAGA AAACAGAGAG AGGCATTCTG AGTCATCTGA CTGGATGAAG 
ACTGTTCCAA GTTACAACCA AACAAATAGC TCCATGGACT TTAGAAATTA TATGATGAGA 
GATGAGACTC TGGAACCACT GCCCAAAAAC TGGGAAATGG CCTACACTGA CACAGGGATG 
ATCTACTTCA TTGACCACAA TACCAAGACA ACCACCTGGT TGGATCCTCG TCTTTGTAAG 
AAAGCCAAAG CCCCTGAAGA CTGTGAAGAT GGAGAGCTTC CTTATGGCTG GGAGAAAATA 
GAGGACCCTC AGTATGGGAC ATACTATGTT GATCACCTTA ACCAGAAAAC CCAGTTTGAA 
AATCCAGTGG AGGAAGCCAA AAGGAAAAAG CAGTTAGGAC AGGTTGAAAT TGGGTCTTCA 
AAACCAGATA TGGAAAAATC ACACTTCACA AGAGATCCAT CCCAGCTTAA AGGTGTCCTT 
GTTCGAGCAT CACTGAAAAA AAGCACAATG GGATTTGGTT TTACTATTAT TGGTGGAGAT 
AGACCTGATG AGTTCCTACA AGTGAAAAAT GTGCTGAAAG ATGGTCCCGC AGCTCAGGAT 
GGGAAAATTG CACCAGGCGA TGTTATTGTA GACATCAATG GCAACTGTGT CCTCGGTCAC 
ACTCATGCAG ATGTTGTCCA GATGTTTCAA TTGGTACCTG TCAATCAGTA TGTAAACCTC 
ACTTTATGTC GTGGTTATCC ACTTCCTGAT GACAGTGAAG ATCCTGTTGT GGACATTGTT 
GCTGCTACCC CTGTCATCAA TGGACAGTCA TTAACCAAGG GAGAGACTTG CATGAATCCT 
CAGGATTTTA AGCCAGGAGC AATGGTTCTG GAGCAGAATG GAAAATCGGG ACACACTTTG 
ACTGGTGATG GTCTCAATGG ACCATCAGAT GCAAGTGAGC AGAGAGTATC CATGGCATCG 
TCAGGCAGCT CCCAGCCTGA ACTAGTGACT ATCCCTTTGA TTAAGGGCCC TAAAGGGTTT 
GGGTTTGCAA TTGCTGACAG CCCTACTGGA CAGAAGGTGA AAATGATACT GGATAGTCAG 
TGGTGTCAAG GCCTTCAGAA AGGAGATATA ATTAAGGAAA TATACCATCA AAATGTGCAG 
AATTTAACAC ATCTCCAAGT GGTAGAGGTG CTAAAGCAGT TTCCAGTAGG TGCTGATGTA 
CCATTGCTTA TCTTAAGAGG AGGTCCTCCT TCACCAACCA AAACTGCCAA AATGAAAACA 
GATAAAAAGG AAAATGCAGG AAGTTTGGAG GCCATAAATG AGCCTATTCC TCAGCCTATG 
CCTTTTCCAC CGAGCATTAT CAGGTCAGGA TCCCCAAAAT TGGATCCTTC TGAGGTCTAC 
CTGAAATCTA AGACTTTATA TGAAGATAAA CCACCAAACA CCAAAGATTT GGATGTTTTT 
CTTCGAAAAC AAGAGTCAGG GTTTGGCTTC AGGGTGCTAG GAGGAGATGG ACCTGACCAG 
TCTATATATA TTGGGGCTAT TATTCCCCTG GGAGCAGCTG AGAAAGATGG TCGGCTCCGC 
GCAGCTGATG AACTAATGTG CATTGATGGA ATTCCTGTTA AAGGGAAATC ACACAAACAA 
GTCTTGGACC TCATGACAAC TGCTGCTCGA AATGGCCATG TGTTACTAAC TGTCAGACGG 
AAGATCTTCT ATGGAGAAAA ACAACCCGAG GACGACAGCT CTCAGGCCTT CATTTCAACA 
CAGAATGGAT CTCCCCGCCT GAACCGGGCA GAGGTCCCAG CCAGGCCTGC ACCCCAGGAG 
CCCTATGATG TTGTCTTGCA ACGAAAAGAA AATGAAGGAT TTGGCTTTGT CATCCTCACC 
TCCAAAAACA AACCACCTCC AGGAGTTATT CCTCATAAAA TTGGCCGAGT CATAGAAGGA 
AGTCCGGCTG ACCGCTGTGG AAAACTGAAA GTTGGAGATC ATATCTCTGC AGTGAATGGG 
CAGTCCATTG TTGAACTGTC TCATGATAAC ATTGTTCAGC TGATCAAAGA TGCTGGTGTC 
ACCGTCACAC TAACGGTCAT TGCTGAAGAA GAGCATCATG GTCCACCATC AGGAACAAAC 
TCAGCCAGGC AAAGCCCAGC CCTGCAGCAC AGGCCCATGG GACAGTCACA GGCCAACCAC 
ATACCTGGGG ACAGAAGTGC CCTAGAAGGT GAAATTGGAA AAGATGTCTC CACTTCTTAC 
AGACATTCTT GGTCAGACCA CAAGCACCTT GCACAGCCTG ACACCGCAGT AATTTCAGTT 
GTAGGCAGTC GGCACAATCA GAACCTTGGT TGTTATCCAG TAGAGCTGGA GAGAGGCCCC 
CGGGGCTTTG GATTCAGCCT CCGAGGGGGG AAGGAGTACA ACATGGGGCT GTTCATCCTT 
CGTCTTGCTG AAGATGGTCC TGCCATCAAA GATGGCAGAA TTCATGTTGG TGACCAGATT 
GTTGAAATCA ATGGGGAACC TACACAAGGA ATCACACATA CTCGAGCAAT TGAGCTCATT 
CAGGCTGGTG GAAATAAAGT TCTTCTTCTT TTGAGGCCAG GAACTGGCTT GATACCTGAC 
CATGGTGATT GGGATATTAA TAATCCTTCG TCTTCAAATG TGATTTATGA TGAACAGTCA 
CCATTACCCC CATCTTCACA TTTTGCTTCC ATATTTGAAG AGTCTCACGT GCCAGTAATT 
GAAGAATCTT TGAGAGTTCA GATATGTGAA AAGGCAGAAG AATTAAAGGA CATTGTGCCT 
GAAAAGAAAA GCACTTTAAA TGAAAATCAG CCTGAGATAA AGCATCAGTC TCTTCTCCAG 
AAAAATGTGA GTAAGAGGGA TCCACCCAGC AGTCATGGGC ACAGTAACAA GAAAAATCTA 
TTAAAAGTAG AAAATGGTGT TACACGAAGA GGTAGATCGG TTAGTCCCAA AAAGCCAGCC 
AGTCAACATT CAGAGGAACA TTTGGATAAG ATTCCTAGTC CTCTAAAAAA TAACCCCAAA 
AGAAGACCCA GAGATCAATC CCTCAGCCCC AGCAAAGGGG AAAATAAAAG TTGTCAGGTC 
AGCACCAGGG CAGGCTCTGG ACAAGATCAG TGCAGAAAAA GCAGAGGTCG GTCGGCCAGC 
CCAAAAAAGC AGCAAAAAAT TGAAGGAAGC AAAGCTCCAT CAAATGCTGA GGCCAAATTA 
TTAGAGGGTA AGAGTCGAAG AATAGCAGGC TATACGGGCA GTAATGCTGA GCAGATCCCA 
GATGGGAAGG AAAAATCAGA CGTCATCAGG AAAGATGCAA AGCAGAATCA GTTGGAAAAA 
AGCAGAACAA GGTCTCCAGA GAAAAAAATC AAAAGAATGG TTGAGAAATC TCTTCCATCC 
AAAATGACTA ATAAGACTAC AAGTAAAGAA GTATCTGAAA ATGAAAAAGG AAAGAAAGTA 
ACCACAGGAG AAACAAGTTC TAGTAACGAT AAAATAGGAG AAAATGTCCA GCTATCAGAA 
AAGAGGCTGA AGCAAGAACC TGAAGAGAAG GTAGTTTCAA ACAAAACAGA AGATCACAAA 
GGGAAAGAAC TAGAGGCAGC TGACAAAAAC AAAGAGACTG GAAGGTTCAA ACCGGAAAGC 
AGTTCTCCAG TTAAGAAAAC ACTGATAACT CCAGGGCCCT GGAAGGTTCC AAGTGGAAAT 
AAAGTCACAG GCACTATTGG TATGGCTGAG AAACGGCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( NM_001142782.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136254.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13683
Clone ID Related Accession (Same CDS sequence)	NM_001142782.2 , NM_001142782.1
Accession Version	NM_001142782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL365225.13, BC130409.1, AK311589.1, AB046854.1 and AA992863.1. On Nov 22, 2018 this sequence version replaced NM_001142782.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000307546.14/ ENSP00000304604.9 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT GATTGGGATA TTAATAATCC TTCGTCTTCA 
AATGTGATTT ATGATGAACA GTCACCATTA CCCCCATCTT CACATTTTGC TTCCATATTT 
GAAGAGTCTC ACGTGCCAGT AATTGAAGAA TCTTTGAGAG TTCAGATATG TGAAAAGGCA 
GAAGAATTAA AGGACATTGT GCCTGAAAAG AAAAGCACTT TAAATGAAAA TCAGCCTGAG 
ATAAAGCATC AGTCTCTTCT CCAGAAAAAT GTGAGTAAGA GGGATCCACC CAGCAGTCAT 
GGGCACAGTA ACAAGAAAAA TCTATTAAAA GTAGAAAATG GTGTTACACG AAGAGGTAGA 
TCGGTTAGTC CCAAAAAGCC AGCCAGTCAA CATTCAGAGG AACATTTGGA TAAGATTCCT 
AGTCCTCTAA AAAATAACCC CAAAAGAAGA CCCAGAGATC AATCCCTCAG CCCCAGCAAA 
GGGGAAAATA AAAGTTGTCA GGTCAGCACC AGGGCAGGCT CTGGACAAGA TCAGTGCAGA 
AAAAGCAGAG GTCGGTCGGC CAGCCCAAAA AAGCAGCAAA AAATTGAAGG AAGCAAAGCT 
CCATCAAATG CTGAGGCCAA ATTATTAGAG GGTAAGAGTC GAAGAATAGC AGGCTATACG 
GGCAGTAATG CTGAGCAGAT CCCAGATGGG AAGGAAAAAT CAGACGTCAT CAGGAAAGAT 
GCAAAGCAGA ATCAGTTGGA AAAAAGCAGA ACAAGGTCTC CAGAGAAAAA AATCAAAAGA 
ATGGTTGAGA AATCTCTTCC ATCCAAAATG ACTAATAAGA CTACAAGTAA AGAAGTATCT 
GAAAATGAAA AAGGAAAGAA AGTAACCACA GGAGAAACAA GTTCTAGTAA CGATAAAATA 
GGAGAAAATG TCCAGCTATC AGAAAAGAGG CTGAAGCAAG AACCTGAAGA GAAGGTAGTT 
TCAAACAAAA CAGAAGATCA CAAAGGGAAA GAACTAGAGG CAGCTGACAA AAACAAAGAG 
ACTGGAAGGT TCAAACCGGA AAGCAGTTCT CCAGTTAAGA AAACACTGAT AACTCCAGGG 
CCCTGGAAGG TTCCAAGTGG AAATAAAGTC ACAGGCACTA TTGGTATGGC TGAGAAACGG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136254.1
CDS	520..4965
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASIFEESHVPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQSLLQKNVSKRDPPSSHGHSNKKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGENKSCQVSTRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYTGSNAEQIPDGKEKSDVIRKDAKQNQLEKSRTRSPEKKIKRMVEKSLPSKMTNKTTSKEVSENEKGKKVTTGETSSSNDKIGENVQLSEKRLKQEPEEKVVSNKTEDHKGKELEAADKNKETGRFKPESSSPVKKTLITPGPWKVPSGNKVTGTIGMAEKRQ

Target ORF information:

RefSeq Version	NM_001142782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142782.2

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT GATTGGGATA TTAATAATCC TTCGTCTTCA 
AATGTGATTT ATGATGAACA GTCACCATTA CCCCCATCTT CACATTTTGC TTCCATATTT 
GAAGAGTCTC ACGTGCCAGT AATTGAAGAA TCTTTGAGAG TTCAGATATG TGAAAAGGCA 
GAAGAATTAA AGGACATTGT GCCTGAAAAG AAAAGCACTT TAAATGAAAA TCAGCCTGAG 
ATAAAGCATC AGTCTCTTCT CCAGAAAAAT GTGAGTAAGA GGGATCCACC CAGCAGTCAT 
GGGCACAGTA ACAAGAAAAA TCTATTAAAA GTAGAAAATG GTGTTACACG AAGAGGTAGA 
TCGGTTAGTC CCAAAAAGCC AGCCAGTCAA CATTCAGAGG AACATTTGGA TAAGATTCCT 
AGTCCTCTAA AAAATAACCC CAAAAGAAGA CCCAGAGATC AATCCCTCAG CCCCAGCAAA 
GGGGAAAATA AAAGTTGTCA GGTCAGCACC AGGGCAGGCT CTGGACAAGA TCAGTGCAGA 
AAAAGCAGAG GTCGGTCGGC CAGCCCAAAA AAGCAGCAAA AAATTGAAGG AAGCAAAGCT 
CCATCAAATG CTGAGGCCAA ATTATTAGAG GGTAAGAGTC GAAGAATAGC AGGCTATACG 
GGCAGTAATG CTGAGCAGAT CCCAGATGGG AAGGAAAAAT CAGACGTCAT CAGGAAAGAT 
GCAAAGCAGA ATCAGTTGGA AAAAAGCAGA ACAAGGTCTC CAGAGAAAAA AATCAAAAGA 
ATGGTTGAGA AATCTCTTCC ATCCAAAATG ACTAATAAGA CTACAAGTAA AGAAGTATCT 
GAAAATGAAA AAGGAAAGAA AGTAACCACA GGAGAAACAA GTTCTAGTAA CGATAAAATA 
GGAGAAAATG TCCAGCTATC AGAAAAGAGG CTGAAGCAAG AACCTGAAGA GAAGGTAGTT 
TCAAACAAAA CAGAAGATCA CAAAGGGAAA GAACTAGAGG CAGCTGACAA AAACAAAGAG 
ACTGGAAGGT TCAAACCGGA AAGCAGTTCT CCAGTTAAGA AAACACTGAT AACTCCAGGG 
CCCTGGAAGG TTCCAAGTGG AAATAAAGTC ACAGGCACTA TTGGTATGGC TGAGAAACGG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGI3 ( NM_001142782.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136254.1 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.

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CloneID	OHu13683
Clone ID Related Accession (Same CDS sequence)	NM_001142782.2 , NM_001142782.1
Accession Version	NM_001142782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC130409.1, AK311589.1, AB046854.1 and AA992863.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT GATTGGGATA TTAATAATCC TTCGTCTTCA 
AATGTGATTT ATGATGAACA GTCACCATTA CCCCCATCTT CACATTTTGC TTCCATATTT 
GAAGAGTCTC ACGTGCCAGT AATTGAAGAA TCTTTGAGAG TTCAGATATG TGAAAAGGCA 
GAAGAATTAA AGGACATTGT GCCTGAAAAG AAAAGCACTT TAAATGAAAA TCAGCCTGAG 
ATAAAGCATC AGTCTCTTCT CCAGAAAAAT GTGAGTAAGA GGGATCCACC CAGCAGTCAT 
GGGCACAGTA ACAAGAAAAA TCTATTAAAA GTAGAAAATG GTGTTACACG AAGAGGTAGA 
TCGGTTAGTC CCAAAAAGCC AGCCAGTCAA CATTCAGAGG AACATTTGGA TAAGATTCCT 
AGTCCTCTAA AAAATAACCC CAAAAGAAGA CCCAGAGATC AATCCCTCAG CCCCAGCAAA 
GGGGAAAATA AAAGTTGTCA GGTCAGCACC AGGGCAGGCT CTGGACAAGA TCAGTGCAGA 
AAAAGCAGAG GTCGGTCGGC CAGCCCAAAA AAGCAGCAAA AAATTGAAGG AAGCAAAGCT 
CCATCAAATG CTGAGGCCAA ATTATTAGAG GGTAAGAGTC GAAGAATAGC AGGCTATACG 
GGCAGTAATG CTGAGCAGAT CCCAGATGGG AAGGAAAAAT CAGACGTCAT CAGGAAAGAT 
GCAAAGCAGA ATCAGTTGGA AAAAAGCAGA ACAAGGTCTC CAGAGAAAAA AATCAAAAGA 
ATGGTTGAGA AATCTCTTCC ATCCAAAATG ACTAATAAGA CTACAAGTAA AGAAGTATCT 
GAAAATGAAA AAGGAAAGAA AGTAACCACA GGAGAAACAA GTTCTAGTAA CGATAAAATA 
GGAGAAAATG TCCAGCTATC AGAAAAGAGG CTGAAGCAAG AACCTGAAGA GAAGGTAGTT 
TCAAACAAAA CAGAAGATCA CAAAGGGAAA GAACTAGAGG CAGCTGACAA AAACAAAGAG 
ACTGGAAGGT TCAAACCGGA AAGCAGTTCT CCAGTTAAGA AAACACTGAT AACTCCAGGG 
CCCTGGAAGG TTCCAAGTGG AAATAAAGTC ACAGGCACTA TTGGTATGGC TGAGAAACGG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136254.1
CDS	182..4627
Translation	MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASIFEESHVPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQSLLQKNVSKRDPPSSHGHSNKKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGENKSCQVSTRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYTGSNAEQIPDGKEKSDVIRKDAKQNQLEKSRTRSPEKKIKRMVEKSLPSKMTNKTTSKEVSENEKGKKVTTGETSSSNDKIGENVQLSEKRLKQEPEEKVVSNKTEDHKGKELEAADKNKETGRFKPESSSPVKKTLITPGPWKVPSGNKVTGTIGMAEKRQ

Target ORF information:

RefSeq Version	NM_001142782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142782.1

ORF Insert Sequence:

ATGTCGAAGA CGCTGAAGAA GAAGAAGCAC TGGCTCAGCA AGGTGCAGGA GTGCGCCGTG 
TCCTGGGCCG GGCCCCCGGG CGACTTCGGC GCGGAGATCC GCGGTGGCGC GGAGCGTGGC 
GAGTTCCCCT ACCTGGGGCG GCTCCGCGAG GAGCCCGGCG GGGGCACCTG CTGCGTCGTC 
TCGGGCAAGG CGCCCAGCCC AGGCGATGTG CTGCTGGAGG TAAACGGGAC GCCTGTCAGC 
GGGCTCACCA ACCGGGACAC CCTGGCTGTC ATCCGCCACT TCCGCGAGCC CATCCGTCTC 
AAGACTGTGA AACCAGGCAA AGTCATTAAT AAAGATTTGC GGCATTACCT AAGTCTTCAG 
TTTCAAAAAG GATCAATTGA CCACAAACTG CAGCAAGTGA TCAGAGATAA TCTCTACTTG 
AGAACCATTC CATGCACTAC AAGGGCCCCC AGGGATGGAG AAGTACCAGG AGTGGATTAT 
AATTTCATTT CCGTTGAACA GTTCAAAGCA CTGGAAGAGA GTGGAGCATT GTTAGAAAGT 
GGGACATATG ATGGAAACTT CTATGGAACT CCCAAGCCTC CAGCAGAACC CAGCCCTTTT 
CAGCCAGATC CAGTTGATCA AGTCCTCTTT GATAATGAGT TTGATGCAGA ATCTCAAAGA 
AAACGAACGA CATCTGTCAG CAAGATGGAA AGAATGGATA GCTCTCTTCC TGAAGAGGAA 
GAAGATGAGG ACAAGGAAGC TATTAATGGC AGTGGAAACG CAGAAAACAG AGAGAGGCAT 
TCTGAGTCAT CTGACTGGAT GAAGACTGTT CCAAGTTACA ACCAAACAAA TAGCTCCATG 
GACTTTAGAA ATTATATGAT GAGAGATGAG ACTCTGGAAC CACTGCCCAA AAACTGGGAA 
ATGGCCTACA CTGACACAGG GATGATCTAC TTCATTGACC ACAATACCAA GACAACCACC 
TGGTTGGATC CTCGTCTTTG TAAGAAAGCC AAAGCCCCTG AAGACTGTGA AGATGGAGAG 
CTTCCTTATG GCTGGGAGAA AATAGAGGAC CCTCAGTATG GGACATACTA TGTTGATCAC 
CTTAACCAGA AAACCCAGTT TGAAAATCCA GTGGAGGAAG CCAAAAGGAA AAAGCAGTTA 
GGACAGGTTG AAATTGGGTC TTCAAAACCA GATATGGAAA AATCACACTT CACAAGAGAT 
CCATCCCAGC TTAAAGGTGT CCTTGTTCGA GCATCACTGA AAAAAAGCAC AATGGGATTT 
GGTTTTACTA TTATTGGTGG AGATAGACCT GATGAGTTCC TACAAGTGAA AAATGTGCTG 
AAAGATGGTC CCGCAGCTCA GGATGGGAAA ATTGCACCAG GCGATGTTAT TGTAGACATC 
AATGGCAACT GTGTCCTCGG TCACACTCAT GCAGATGTTG TCCAGATGTT TCAATTGGTA 
CCTGTCAATC AGTATGTAAA CCTCACTTTA TGTCGTGGTT ATCCACTTCC TGATGACAGT 
GAAGATCCTG TTGTGGACAT TGTTGCTGCT ACCCCTGTCA TCAATGGACA GTCATTAACC 
AAGGGAGAGA CTTGCATGAA TCCTCAGGAT TTTAAGCCAG GAGCAATGGT TCTGGAGCAG 
AATGGAAAAT CGGGACACAC TTTGACTGGT GATGGTCTCA ATGGACCATC AGATGCAAGT 
GAGCAGAGAG TATCCATGGC ATCGTCAGGC AGCTCCCAGC CTGAACTAGT GACTATCCCT 
TTGATTAAGG GCCCTAAAGG GTTTGGGTTT GCAATTGCTG ACAGCCCTAC TGGACAGAAG 
GTGAAAATGA TACTGGATAG TCAGTGGTGT CAAGGCCTTC AGAAAGGAGA TATAATTAAG 
GAAATATACC ATCAAAATGT GCAGAATTTA ACACATCTCC AAGTGGTAGA GGTGCTAAAG 
CAGTTTCCAG TAGGTGCTGA TGTACCATTG CTTATCTTAA GAGGAGGTCC TCCTTCACCA 
ACCAAAACTG CCAAAATGAA AACAGATAAA AAGGAAAATG CAGGAAGTTT GGAGGCCATA 
AATGAGCCTA TTCCTCAGCC TATGCCTTTT CCACCGAGCA TTATCAGGTC AGGATCCCCA 
AAATTGGATC CTTCTGAGGT CTACCTGAAA TCTAAGACTT TATATGAAGA TAAACCACCA 
AACACCAAAG ATTTGGATGT TTTTCTTCGA AAACAAGAGT CAGGGTTTGG CTTCAGGGTG 
CTAGGAGGAG ATGGACCTGA CCAGTCTATA TATATTGGGG CTATTATTCC CCTGGGAGCA 
GCTGAGAAAG ATGGTCGGCT CCGCGCAGCT GATGAACTAA TGTGCATTGA TGGAATTCCT 
GTTAAAGGGA AATCACACAA ACAAGTCTTG GACCTCATGA CAACTGCTGC TCGAAATGGC 
CATGTGTTAC TAACTGTCAG ACGGAAGATC TTCTATGGAG AAAAACAACC CGAGGACGAC 
AGCTCTCAGG CCTTCATTTC AACACAGAAT GGATCTCCCC GCCTGAACCG GGCAGAGGTC 
CCAGCCAGGC CTGCACCCCA GGAGCCCTAT GATGTTGTCT TGCAACGAAA AGAAAATGAA 
GGATTTGGCT TTGTCATCCT CACCTCCAAA AACAAACCAC CTCCAGGAGT TATTCCTCAT 
AAAATTGGCC GAGTCATAGA AGGAAGTCCG GCTGACCGCT GTGGAAAACT GAAAGTTGGA 
GATCATATCT CTGCAGTGAA TGGGCAGTCC ATTGTTGAAC TGTCTCATGA TAACATTGTT 
CAGCTGATCA AAGATGCTGG TGTCACCGTC ACACTAACGG TCATTGCTGA AGAAGAGCAT 
CATGGTCCAC CATCAGGAAC AAACTCAGCC AGGCAAAGCC CAGCCCTGCA GCACAGGCCC 
ATGGGACAGT CACAGGCCAA CCACATACCT GGGGACAGAA GTGCCCTAGA AGGTGAAATT 
GGAAAAGATG TCTCCACTTC TTACAGACAT TCTTGGTCAG ACCACAAGCA CCTTGCACAG 
CCTGACACCG CAGTAATTTC AGTTGTAGGC AGTCGGCACA ATCAGAACCT TGGTTGTTAT 
CCAGTAGAGC TGGAGAGAGG CCCCCGGGGC TTTGGATTCA GCCTCCGAGG GGGGAAGGAG 
TACAACATGG GGCTGTTCAT CCTTCGTCTT GCTGAAGATG GTCCTGCCAT CAAAGATGGC 
AGAATTCATG TTGGTGACCA GATTGTTGAA ATCAATGGGG AACCTACACA AGGAATCACA 
CATACTCGAG CAATTGAGCT CATTCAGGCT GGTGGAAATA AAGTTCTTCT TCTTTTGAGG 
CCAGGAACTG GCTTGATACC TGACCATGGT GATTGGGATA TTAATAATCC TTCGTCTTCA 
AATGTGATTT ATGATGAACA GTCACCATTA CCCCCATCTT CACATTTTGC TTCCATATTT 
GAAGAGTCTC ACGTGCCAGT AATTGAAGAA TCTTTGAGAG TTCAGATATG TGAAAAGGCA 
GAAGAATTAA AGGACATTGT GCCTGAAAAG AAAAGCACTT TAAATGAAAA TCAGCCTGAG 
ATAAAGCATC AGTCTCTTCT CCAGAAAAAT GTGAGTAAGA GGGATCCACC CAGCAGTCAT 
GGGCACAGTA ACAAGAAAAA TCTATTAAAA GTAGAAAATG GTGTTACACG AAGAGGTAGA 
TCGGTTAGTC CCAAAAAGCC AGCCAGTCAA CATTCAGAGG AACATTTGGA TAAGATTCCT 
AGTCCTCTAA AAAATAACCC CAAAAGAAGA CCCAGAGATC AATCCCTCAG CCCCAGCAAA 
GGGGAAAATA AAAGTTGTCA GGTCAGCACC AGGGCAGGCT CTGGACAAGA TCAGTGCAGA 
AAAAGCAGAG GTCGGTCGGC CAGCCCAAAA AAGCAGCAAA AAATTGAAGG AAGCAAAGCT 
CCATCAAATG CTGAGGCCAA ATTATTAGAG GGTAAGAGTC GAAGAATAGC AGGCTATACG 
GGCAGTAATG CTGAGCAGAT CCCAGATGGG AAGGAAAAAT CAGACGTCAT CAGGAAAGAT 
GCAAAGCAGA ATCAGTTGGA AAAAAGCAGA ACAAGGTCTC CAGAGAAAAA AATCAAAAGA 
ATGGTTGAGA AATCTCTTCC ATCCAAAATG ACTAATAAGA CTACAAGTAA AGAAGTATCT 
GAAAATGAAA AAGGAAAGAA AGTAACCACA GGAGAAACAA GTTCTAGTAA CGATAAAATA 
GGAGAAAATG TCCAGCTATC AGAAAAGAGG CTGAAGCAAG AACCTGAAGA GAAGGTAGTT 
TCAAACAAAA CAGAAGATCA CAAAGGGAAA GAACTAGAGG CAGCTGACAA AAACAAAGAG 
ACTGGAAGGT TCAAACCGGA AAGCAGTTCT CCAGTTAAGA AAACACTGAT AACTCCAGGG 
CCCTGGAAGG TTCCAAGTGG AAATAAAGTC ACAGGCACTA TTGGTATGGC TGAGAAACGG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variation and expression levels of tight junction genes identifies association between MAGI3 and inflammatory bowel disease.
BMC gastroenterology17(1)68(2017 May)
Nor?n E,Almer S,S?derman J

Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer.
eLife5(2016 05)
Ni TK,Kuperwasser C

MAGI3 negatively regulates Wnt/?-catenin signaling and suppresses malignant phenotypes of glioma cells.
Oncotarget6(34)35851-65(2015 Nov)
Ma Q,Yang Y,Feng D,Zheng S,Meng R,Fa P,Zhao C,Liu H,Song R,Tao T,Yang L,Dai J,Wang S,Jiang WG,He J

MAGI3 Suppresses Glioma Cell Proliferation via Upregulation of PTEN Expression.
Biomedical and environmental sciences : BES28(7)502-9(2015 Jul)
Ma Q,Zhang Y,Meng R,Xie KM,Xiong Y,Lin S,He ZL,Tao T,Yang Y,Zhao JZ,He JQ

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MAGI3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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