UPF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UPF2
Entrez Gene ID	26019
Full Name	UPF2, regulator of nonsense mediated mRNA decay
Synonyms	HUPF2,RENT2,smg-3
General protein information	Preferred Names UPF2, regulator of nonsense mediated mRNA decay Names regulator of nonsense transcripts 2 FRS2/UPF2/LEMD3 fusion LEMD3/UPF2 fusion UPF2 regulator of nonsense transcripts homolog nonsense mRNA reducing factor 2 smg-3 homolog, nonsense mediated mRNA decay factor up-frameshift suppressor 2 homolog yeast Upf2p homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p14
Summary	This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	605529

mRNA and Protein(s)

mRNA	Protein	Name
XM_017016034.1	XP_016871523.1	regulator of nonsense transcripts 2 isoform X1
XM_011519449.2	XP_011517751.1	regulator of nonsense transcripts 2 isoform X2
NM_080599.2	NP_542166.1	regulator of nonsense transcripts 2
NM_015542.3	NP_056357.1	regulator of nonsense transcripts 2
NM_015542.4	NP_056357.1	regulator of nonsense transcripts 2
XM_011519449.3	XP_011517751.1	regulator of nonsense transcripts 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UPF2	XP_005214170.1
Homo sapiens (human)	UPF2	NP_542166.1
Rattus norvegicus (Norway rat)	Upf2	NP_001101891.1
Danio rerio (zebrafish)	upf2	XP_697460.6
Drosophila melanogaster (fruit fly)	Upf2	NP_572434.1
Neurospora crassa	NCU05267	XP_961757.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000337	XP_310780.5
Saccharomyces cerevisiae (baker's yeast)	NMD2	NP_011944.2
Eremothecium gossypii	AGOS_AGR364C	NP_987030.2
Pan troglodytes (chimpanzee)	UPF2	XP_507656.3
	UPF2	XP_002805601.1
Gallus gallus (chicken)	UPF2	XP_004937542.1
Kluyveromyces lactis	KLLA0D13156g	XP_453649.1
Magnaporthe oryzae (rice blast fungus)	MGG_06063	XP_003711916.1
Arabidopsis thaliana (thale cress)	AT2G39260	NP_181459.4
Canis lupus familiaris (dog)	UPF2	XP_005617199.1
Mus musculus (house mouse)	Upf2	NP_001074601.1
Schizosaccharomyces pombe (fission yeast)	upf2	NP_593784.1
Xenopus tropicalis (tropical clawed frog)	upf2	NP_001121475.1

Gene Ontology
Bibliography

Related articles in PubMed

Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex.
L?pez-Perrote A, Casta?o R, Melero R, Zamarro T, Kurosawa H, Ohnishi T, Uchiyama A, Aoyagi K, Buchwald G, Kataoka N, Yamashita A, Llorca O
Nucleic acids research44(4)1909-23(2016 Feb)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.
Melero R, Uchiyama A, Casta?o R, Kataoka N, Kurosawa H, Ohno S, Yamashita A, Llorca O
Structure (London, England : 1993)22(8)1105-1119(2014 Aug)

Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S
Nucleic acids research42(4)2673-86(2014 Feb)

Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells.
Zhao X, Nogawa A, Matsunaga T, Takegami T, Nakagawa H, Ishigaki Y
International journal of oncology44(1)222-8(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we find that the interaction of UPF2 with UPF3b interferes with the assembly of the UPF2-eRF3 complex, and that UPF2 binds UPF3b more strongly than eRF3
Title: Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex.

UPF2 binds the FRB domain of SMG1, a region that regulates the related mTOR kinase.
Title: Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.

UPF2 MIF4G-1 and MIF4G-2 domains appear to have a crucial scaffolding role, while the MIF4G-3 domain is the key module required for triggering nonsense-mediated decay.
Title: Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.

This study demonstrated the quantitative regulation of Upf1 and Upf2 proteins by ubiquitin-proteasome system and SMG1.
Title: Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells.

Data show that upon binding to Upf2, the regulatory CH domain of Upf1 undergoes a large conformational change, causing the catalytic helicase domain to bind RNA less extensively and triggering its helicase activity.
Title: Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.

The following UPF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UPF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23886	NM_015542.4 Latest version!	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu23886	NM_080599.2 Latest version!	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu91271	XM_017016034.1 Latest version!	Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu66723	XM_011519449.3 Latest version!	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu23886	NM_015542.3	Homo sapiens UPF2, regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu66723	XM_011519449.2	Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UPF2 ( NM_080599.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542166.1 Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23886
Clone ID Related Accession (Same CDS sequence)	NM_080599.2 , NM_015542.3 , NM_015542.4
Accession Version	NM_080599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB065422.1, BC114964.1 and AF301013.1. This sequence is a reference standard in the RefSeqGene project. On Dec 9, 2011 this sequence version replaced NM_080599.1. Transcript Variant: This variant (1) contains a different 5' UTR than variant 2 and is the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.72386.1, SRR1803617.164467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542166.1
CDS	276..4094
Translation	MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	NM_080599.2
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080599.2

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF2 ( NM_015542.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056357.1 Homo sapiens UPF2, regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23886
Clone ID Related Accession (Same CDS sequence)	NM_080599.2 , NM_015542.3 , NM_015542.4
Accession Version	NM_015542.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188353.1, BC114964.1 and AF301013.1. On Dec 9, 2011 this sequence version replaced NM_015542.2. Transcript Variant: This variant (2) contains a different 5' UTR than variant 1 and is the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF301013.1, SRR1803612.29577.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056357.1
CDS	106..3924
Translation	MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	NM_015542.3
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2, regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015542.3

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF2 ( NM_015542.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056357.1 Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.

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CloneID	OHu23886
Clone ID Related Accession (Same CDS sequence)	NM_080599.2 , NM_015542.3 , NM_015542.4
Accession Version	NM_015542.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188353.1, BC114964.1 and AF301013.1. On Nov 23, 2018 this sequence version replaced NM_015542.3. Transcript Variant: This variant (2) contains a different 5' UTR than variant 1 and is the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF301013.1, SRR1660805.177487.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357604.10/ ENSP00000350221.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056357.1
CDS	74..3892
Translation	MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	NM_015542.4
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015542.4

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGATG CTTCTGTCAG ACTTCTCTCA TCACCATATT 
GAAATGGCAT GCACCCTGCT GGAGACATGT GGACGGTTTC TTTTCAGATC TCCAGAATCT 
CACCTGAGGA CCAGTGTACT TTTGGAGCAA ATGATGAGAA AGAAGCAAGC AATGCATCTT 
GATGCGAGAT ACGTCACAAT GGTAGAGAAT GCATATTACT ACTGCAACCC ACCTCCAGCT 
GAAAAAACCG TGAAAAAGAA ACGTCCTCCT CTCCAGGAAT ATGTCCGGAA ACTTTTGTAC 
AAGGATCTCT CTAAGGTTAC CACCGAGAAG GTTTTGAGAC AGATGCGAAA GCTGCCCTGG 
CAGGACCAAG AAGTGAAAGA CTATGTTATT TGTTGTATGA TAAACATCTG GAATGTGAAA 
TATAATAGTA TTCATTGTGT AGCCAACCTC TTAGCAGGAC TAGTGCTCTA CCAAGAGGAT 
GTTGGGATCC ACGTTGTGGA TGGAGTGTTA GAAGATATTC GATTAGGAAT GGAGGTTAAT 
CAACCTAAAT TTAATCAGAG GCGCATCAGC AGTGCCAAGT TCTTAGGAGA ACTTTACAAT 
TACCGAATGG TGGAATCAGC TGTTATTTTC AGAACTCTGT ATTCTTTTAC CTCATTTGGT 
GTTAATCCTG ATGGCTCTCC AAGTTCCCTG GACCCACCTG AGCATCTTTT CAGAATTAGA 
CTCGTATGCA CTATTCTGGA CACATGTGGC CAGTACTTTG ACAGAGGTTC CAGTAAACGA 
AAACTTGATT GTTTCCTTGT ATATTTTCAG CGTTATGTTT GGTGGAAGAA AAGTTTGGAG 
GTTTGGACAA AAGACCATCC ATTTCCTATT GATATAGATT ACATGATCAG TGATACACTA 
GAACTGCTAA GACCAAAGAT CAAACTCTGT AATTCTCTGG AAGAATCCAT CAGGCAGGTA 
CAAGACTTGG AACGAGAATT CTTAATAAAA CTAGGCCTAG TAAATGACAA AGACTCAAAA 
GATTCTATGA CAGAAGGAGA AAATCTTGAA GAGGATGAAG AAGAAGAAGA AGGTGGGGCT 
GAAACAGAAG AACAATCTGG AAATGAAAGT GAAGTAAATG AGCCAGAAGA AGAGGAGGGT 
TCTGATAATG ATGATGATGA GGGAGAAGAA GAGGAGGAAG AGAATACAGA TTACCTTACA 
GATTCCAATA AGGAAAATGA AACCGATGAA GAGAATACTG AGGTAATGAT TAAAGGCGGT 
GGACTTAAGC ATGTACCTTG TGTAGAAGAT GAGGACTTCA TTCAAGCTCT GGATAAAATG 
ATGCTAGAAA ATCTACAGCA ACGAAGTGGT GAATCTGTTA AAGTGCACCA ACTAGATGTT 
GCCATTCCTT TGCATCTCAA AAGCCAGCTG AGGAAAGGGC CCCCACTGGG AGGTGGGGAA 
GGAGAGGCTG AGTCTGCAGA CACAATGCCG TTTGTCATGT TAACAAGAAA AGGCAATAAA 
CAGCAGTTTA AGATCCTTAA TGTACCCATG TCCTCTCAAC TTGCTGCAAA TCACTGGAAC 
CAGCAACAGG CAGAACAAGA AGAGAGGATG AGAATGAAAA AGCTCACACT AGATATCAAT 
GAACGGCAAG AACAAGAAGA TTATCAAGAA ATGTTGCAGT CTCTTGCACA GCGCCCAGCT 
CCAGCAAACA CCAATCGTGA GAGGCGGCCT CGCTACCAAC ATCCGAAGGG AGCACCTAAT 
GCAGATCTAA TCTTTAAGAC TGGTGGGAGG AGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF2 ( XM_017016034.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871523.1 Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X1, mRNA.

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CloneID	OHu91271
Clone ID Related Accession (Same CDS sequence)	XM_017016034.1
Accession Version	XM_017016034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTACC AGAAGCTGCT GGCAAATTCT CAATCCTTAG CAGACCTTTT GGATGAAAAT 
ATGCCAGATC TTCCTCAAGA CAAACCAACA CCAGAAGAAC ATGGGCCTGG AATTGATATA 
TTCACACCTG GTAAACCTGG AGAATATGAC TTGGAAGGTG GTATATGGGA AGATGAAGAT 
GCTCGGAATT TTTATGAGAA CCTCATTGAT TTGAAGGCTT TTGTCCCAGC CATCTTGTTT 
AAAGACAATG AAAAAAGTTG TCAGAATAAA GAGTCCAACA AAGATGATAC CAAAGAGGCA 
AAAGAATCTA AGGAGAATAA GGAGGTATCA AGTCCCGATG ATTTGGAACT TGAGTTGGAG 
AATCTAGAAA TTAATGATGA CACCTTAGAA TTAGAGGGTG GAGATGAAGC TGAAGATCTT 
ACAAAGAAAC TTCTTGATGA ACAAGAACAA GAAGATGAGG AAGCCAGCAC TGGATCTCAT 
CTCAAGCTCA TAGTAGATGC TTTCCTACAG CAGTTACCCA ACTGTGTCAA CCGAGATCTG 
ATAGACAAGG CAGCAATGGA TTTTTGCATG AACATGAACA CAAAAGCAAA CAGGAAGAAG 
TTGGTACGGG CACTCTTCAT AGTTCCTAGA CAAAGGTTGG ATTTGCTACC ATTTTATGCA 
AGATTGGTTG CTACATTGCA TCCCTGCATG TCTGATGTAG CAGAGGATCT TTGTTCCATG 
CTGAGGGGGG ATTTCAGATT TCATGTACGG AAAAAGGACC AGATCAATAT TGAAACAAAG 
AATAAAACTG TTCGTTTTAT AGGAGAACTA ACTAAGTTTA AGATGTTCAC CAAAAATGAC 
ACACTGCATT GTTTAAAGAT GCTTCTGTCA GACTTCTCTC ATCACCATAT TGAAATGGCA 
TGCACCCTGC TGGAGACATG TGGACGGTTT CTTTTCAGAT CTCCAGAATC TCACCTGAGG 
ACCAGTGTAC TTTTGGAGCA AATGATGAGA AAGAAGCAAG CAATGCATCT TGATGCGAGA 
TACGTCACAA TGGTAGAGAA TGCATATTAC TACTGCAACC CACCTCCAGC TGAAAAAACC 
GTGAAAAAGA AACGTCCTCC TCTCCAGGAA TATGTCCGGA AACTTTTGTA CAAGGATCTC 
TCTAAGGTTA CCACCGAGAA GGTTTTGAGA CAGATGCGAA AGCTGCCCTG GCAGGACCAA 
GAAGTGAAAG ACTATGTTAT TTGTTGTATG ATAAACATCT GGAATGTGAA ATATAATAGT 
ATTCATTGTG TAGCCAACCT CTTAGCAGGA CTAGTGCTCT ACCAAGAGGA TGTTGGGATC 
CACGTTGTGG ATGGAGTGTT AGAAGATATT CGATTAGGAA TGGAGGTTAA TCAACCTAAA 
TTTAATCAGA GGCGCATCAG CAGTGCCAAG TTCTTAGGAG AACTTTACAA TTACCGAATG 
GTGGAATCAG CTGTTATTTT CAGAACTCTG TATTCTTTTA CCTCATTTGG TGTTAATCCT 
GATGGCTCTC CAAGTTCCCT GGACCCACCT GAGCATCTTT TCAGAATTAG ACTCGTATGC 
ACTATTCTGG ACACATGTGG CCAGTACTTT GACAGAGGTT CCAGTAAACG AAAACTTGAT 
TGTTTCCTTG TATATTTTCA GCGTTATGTT TGGTGGAAGA AAAGTTTGGA GGTTTGGACA 
AAAGACCATC CATTTCCTAT TGATATAGAT TACATGATCA GTGATACACT AGAACTGCTA 
AGACCAAAGA TCAAACTCTG TAATTCTCTG GAAGAATCCA TCAGGCAGGT ACAAGACTTG 
GAACGAGAAT TCTTAATAAA ACTAGGCCTA GTAAATGACA AAGACTCAAA AGATTCTATG 
ACAGAAGGAG AAAATCTTGA AGAGGATGAA GAAGAAGAAG AAGGTGGGGC TGAAACAGAA 
GAACAATCTG GAAATGAAAG TGAAGTAAAT GAGCCAGAAG AAGAGGAGGG TTCTGATAAT 
GATGATGATG AGGGAGAAGA AGAGGAGGAA GAGAATACAG ATTACCTTAC AGATTCCAAT 
AAGGAAAATG AAACCGATGA AGAGAATACT GAGGTAATGA TTAAAGGCGG TGGACTTAAG 
CATGTACCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGATAAAAT GATGCTAGAA 
AATCTACAGC AACGAAGTGG TGAATCTGTT AAAGTGCACC AACTAGATGT TGCCATTCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCACTGG GAGGTGGGGA AGGAGAGGCT 
GAGTCTGCAG ACACAATGCC GTTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATCCTTA ATGTACCCAT GTCCTCTCAA CTTGCTGCAA ATCACTGGAA CCAGCAACAG 
GCAGAACAAG AAGAGAGGAT GAGAATGAAA AAGCTCACAC TAGATATCAA TGAACGGCAA 
GAACAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACCAATCGTG AGAGGCGGCC TCGCTACCAA CATCCGAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871523.1
CDS	971..3580
Translation	MSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNESEVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_017016034.1
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016034.1

ORF Insert Sequence:

ATGTCTTACC AGAAGCTGCT GGCAAATTCT CAATCCTTAG CAGACCTTTT GGATGAAAAT 
ATGCCAGATC TTCCTCAAGA CAAACCAACA CCAGAAGAAC ATGGGCCTGG AATTGATATA 
TTCACACCTG GTAAACCTGG AGAATATGAC TTGGAAGGTG GTATATGGGA AGATGAAGAT 
GCTCGGAATT TTTATGAGAA CCTCATTGAT TTGAAGGCTT TTGTCCCAGC CATCTTGTTT 
AAAGACAATG AAAAAAGTTG TCAGAATAAA GAGTCCAACA AAGATGATAC CAAAGAGGCA 
AAAGAATCTA AGGAGAATAA GGAGGTATCA AGTCCCGATG ATTTGGAACT TGAGTTGGAG 
AATCTAGAAA TTAATGATGA CACCTTAGAA TTAGAGGGTG GAGATGAAGC TGAAGATCTT 
ACAAAGAAAC TTCTTGATGA ACAAGAACAA GAAGATGAGG AAGCCAGCAC TGGATCTCAT 
CTCAAGCTCA TAGTAGATGC TTTCCTACAG CAGTTACCCA ACTGTGTCAA CCGAGATCTG 
ATAGACAAGG CAGCAATGGA TTTTTGCATG AACATGAACA CAAAAGCAAA CAGGAAGAAG 
TTGGTACGGG CACTCTTCAT AGTTCCTAGA CAAAGGTTGG ATTTGCTACC ATTTTATGCA 
AGATTGGTTG CTACATTGCA TCCCTGCATG TCTGATGTAG CAGAGGATCT TTGTTCCATG 
CTGAGGGGGG ATTTCAGATT TCATGTACGG AAAAAGGACC AGATCAATAT TGAAACAAAG 
AATAAAACTG TTCGTTTTAT AGGAGAACTA ACTAAGTTTA AGATGTTCAC CAAAAATGAC 
ACACTGCATT GTTTAAAGAT GCTTCTGTCA GACTTCTCTC ATCACCATAT TGAAATGGCA 
TGCACCCTGC TGGAGACATG TGGACGGTTT CTTTTCAGAT CTCCAGAATC TCACCTGAGG 
ACCAGTGTAC TTTTGGAGCA AATGATGAGA AAGAAGCAAG CAATGCATCT TGATGCGAGA 
TACGTCACAA TGGTAGAGAA TGCATATTAC TACTGCAACC CACCTCCAGC TGAAAAAACC 
GTGAAAAAGA AACGTCCTCC TCTCCAGGAA TATGTCCGGA AACTTTTGTA CAAGGATCTC 
TCTAAGGTTA CCACCGAGAA GGTTTTGAGA CAGATGCGAA AGCTGCCCTG GCAGGACCAA 
GAAGTGAAAG ACTATGTTAT TTGTTGTATG ATAAACATCT GGAATGTGAA ATATAATAGT 
ATTCATTGTG TAGCCAACCT CTTAGCAGGA CTAGTGCTCT ACCAAGAGGA TGTTGGGATC 
CACGTTGTGG ATGGAGTGTT AGAAGATATT CGATTAGGAA TGGAGGTTAA TCAACCTAAA 
TTTAATCAGA GGCGCATCAG CAGTGCCAAG TTCTTAGGAG AACTTTACAA TTACCGAATG 
GTGGAATCAG CTGTTATTTT CAGAACTCTG TATTCTTTTA CCTCATTTGG TGTTAATCCT 
GATGGCTCTC CAAGTTCCCT GGACCCACCT GAGCATCTTT TCAGAATTAG ACTCGTATGC 
ACTATTCTGG ACACATGTGG CCAGTACTTT GACAGAGGTT CCAGTAAACG AAAACTTGAT 
TGTTTCCTTG TATATTTTCA GCGTTATGTT TGGTGGAAGA AAAGTTTGGA GGTTTGGACA 
AAAGACCATC CATTTCCTAT TGATATAGAT TACATGATCA GTGATACACT AGAACTGCTA 
AGACCAAAGA TCAAACTCTG TAATTCTCTG GAAGAATCCA TCAGGCAGGT ACAAGACTTG 
GAACGAGAAT TCTTAATAAA ACTAGGCCTA GTAAATGACA AAGACTCAAA AGATTCTATG 
ACAGAAGGAG AAAATCTTGA AGAGGATGAA GAAGAAGAAG AAGGTGGGGC TGAAACAGAA 
GAACAATCTG GAAATGAAAG TGAAGTAAAT GAGCCAGAAG AAGAGGAGGG TTCTGATAAT 
GATGATGATG AGGGAGAAGA AGAGGAGGAA GAGAATACAG ATTACCTTAC AGATTCCAAT 
AAGGAAAATG AAACCGATGA AGAGAATACT GAGGTAATGA TTAAAGGCGG TGGACTTAAG 
CATGTACCTT GTGTAGAAGA TGAGGACTTC ATTCAAGCTC TGGATAAAAT GATGCTAGAA 
AATCTACAGC AACGAAGTGG TGAATCTGTT AAAGTGCACC AACTAGATGT TGCCATTCCT 
TTGCATCTCA AAAGCCAGCT GAGGAAAGGG CCCCCACTGG GAGGTGGGGA AGGAGAGGCT 
GAGTCTGCAG ACACAATGCC GTTTGTCATG TTAACAAGAA AAGGCAATAA ACAGCAGTTT 
AAGATCCTTA ATGTACCCAT GTCCTCTCAA CTTGCTGCAA ATCACTGGAA CCAGCAACAG 
GCAGAACAAG AAGAGAGGAT GAGAATGAAA AAGCTCACAC TAGATATCAA TGAACGGCAA 
GAACAAGAAG ATTATCAAGA AATGTTGCAG TCTCTTGCAC AGCGCCCAGC TCCAGCAAAC 
ACCAATCGTG AGAGGCGGCC TCGCTACCAA CATCCGAAGG GAGCACCTAA TGCAGATCTA 
ATCTTTAAGA CTGGTGGGAG GAGACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF2 ( XM_011519449.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517751.1 Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66723
Clone ID Related Accession (Same CDS sequence)	XM_011519449.2 , XM_011519449.3
Accession Version	XM_011519449.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGAAA ATTGGAAATC CGAAACATTT CTGGTCCCAG 
GCATTTCAGA TAAGGGATAC TTCACCTGTA GCAAGAAATG GAAGGCAATT GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517751.1
CDS	154..2310
Translation	MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKKIGNPKHFWSQAFQIRDTSPVARNGRQLG

Target ORF information:

RefSeq Version	XM_011519449.2
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519449.2

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGAAA ATTGGAAATC CGAAACATTT CTGGTCCCAG 
GCATTTCAGA TAAGGGATAC TTCACCTGTA GCAAGAAATG GAAGGCAATT GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF2 ( XM_011519449.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517751.1 Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant X1, mRNA.

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CloneID	OHu66723
Clone ID Related Accession (Same CDS sequence)	XM_011519449.2 , XM_011519449.3
Accession Version	XM_011519449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGAAA ATTGGAAATC CGAAACATTT CTGGTCCCAG 
GCATTTCAGA TAAGGGATAC TTCACCTGTA GCAAGAAATG GAAGGCAATT GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517751.1
CDS	106..2262
Translation	MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKKIGNPKHFWSQAFQIRDTSPVARNGRQLG

Target ORF information:

RefSeq Version	XM_011519449.3
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519449.3

ORF Insert Sequence:

ATGCCAGCTG AGCGTAAAAA GCCAGCAAGT ATGGAAGAAA AAGACTCTTT ACCAAACAAC 
AAGGAAAAAG ACTGCAGTGA AAGGCGGACA GTGAGCAGCA AGGAGAGGCC AAAAGACGAT 
ATCAAGCTCA CTGCCAAGAA GGAGGTCAGC AAGGCCCCTG AAGACAAGAA GAAGAGACTG 
GAAGATGATA AGAGAAAAAA GGAAGACAAG GAACGCAAGA AAAAAGACGA AGAAAAGGTG 
AAGGCAGAGG AAGAATCAAA GAAAAAAGAA GAGGAAGAAA AAAAGAAACA TCAAGAGGAA 
GAGAGAAAGA AGCAAGAAGA GCAGGCCAAA CGTCAGCAAG AAGAAGAAGC AGCTGCTCAG 
ATGAAAGAAA AAGAAGAATC CATTCAGCTT CATCAGGAAG CTTGGGAACG ACATCATTTA 
AGAAAGGAAC TTCGTAGCAA AAACCAAAAT GCTCCGGACA GCCGACCAGA GGAAAACTTC 
TTCAGCCGCC TCGACTCAAG TTTGAAGAAA AATACTGCTT TTGTCAAGAA ACTAAAAACT 
ATTACAGAAC AACAGAGAGA CTCCTTGTCC CATGATTTTA ATGGCCTAAA TTTAAGCAAA 
TACATTGCAG AAGCTGTAGC TTCCATCGTG GAAGCAAAAC TAAAAATCTC TGATGTGAAC 
TGTGCTGTGC ACCTCTGCTC TCTCTTTCAC CAGCGTTATG CTGACTTTGC CCCATCACTT 
CTTCAGGTCT GGAAAAAACA TTTTGAAGCA AGGAAAGAGG AGAAAACACC TAACATCACC 
AAGTTAAGAA CTGATTTGCG TTTTATTGCA GAATTGACAA TAGTTGGGAT TTTCACTGAC 
AAGGAAGGTC TTTCCTTAAT CTATGAACAG CTAAAAAATA TTATTAATGC TGATCGGGAG 
TCCCACACTC ATGTCTCTGT AGTGATTAGT TTCTGTCGAC ATTGTGGAGA TGATATTGCT 
GGACTTGTAC CAAGGAAAGT AAAGAGTGCT GCAGAGAAGT TTAATTTGAG TTTTCCTCCT 
AGTGAGATAA TTAGTCCAGA GAAACAACAG CCCTTCCAGA ATCTTTTAAA AGAGTACTTT 
ACGTCTTTGA CCAAACACCT GAAAAGGGAC CACAGGGAGC TCCAGAATAC TGAGAGACAA 
AACAGGCGCA TTCTACATTC TAAAGGGGAG CTCAGTGAAG ATAGACATAA ACAGTATGAG 
GAATTTGCTA TGTCTTACCA GAAGCTGCTG GCAAATTCTC AATCCTTAGC AGACCTTTTG 
GATGAAAATA TGCCAGATCT TCCTCAAGAC AAACCAACAC CAGAAGAACA TGGGCCTGGA 
ATTGATATAT TCACACCTGG TAAACCTGGA GAATATGACT TGGAAGGTGG TATATGGGAA 
GATGAAGATG CTCGGAATTT TTATGAGAAC CTCATTGATT TGAAGGCTTT TGTCCCAGCC 
ATCTTGTTTA AAGACAATGA AAAAAGTTGT CAGAATAAAG AGTCCAACAA AGATGATACC 
AAAGAGGCAA AAGAATCTAA GGAGAATAAG GAGGTATCAA GTCCCGATGA TTTGGAACTT 
GAGTTGGAGA ATCTAGAAAT TAATGATGAC ACCTTAGAAT TAGAGGGTGG AGATGAAGCT 
GAAGATCTTA CAAAGAAACT TCTTGATGAA CAAGAACAAG AAGATGAGGA AGCCAGCACT 
GGATCTCATC TCAAGCTCAT AGTAGATGCT TTCCTACAGC AGTTACCCAA CTGTGTCAAC 
CGAGATCTGA TAGACAAGGC AGCAATGGAT TTTTGCATGA ACATGAACAC AAAAGCAAAC 
AGGAAGAAGT TGGTACGGGC ACTCTTCATA GTTCCTAGAC AAAGGTTGGA TTTGCTACCA 
TTTTATGCAA GATTGGTTGC TACATTGCAT CCCTGCATGT CTGATGTAGC AGAGGATCTT 
TGTTCCATGC TGAGGGGGGA TTTCAGATTT CATGTACGGA AAAAGGACCA GATCAATATT 
GAAACAAAGA ATAAAACTGT TCGTTTTATA GGAGAACTAA CTAAGTTTAA GATGTTCACC 
AAAAATGACA CACTGCATTG TTTAAAGAAA ATTGGAAATC CGAAACATTT CTGGTCCCAG 
GCATTTCAGA TAAGGGATAC TTCACCTGTA GCAAGAAATG GAAGGCAATT GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex.
Nucleic acids research44(4)1909-23(2016 Feb)
L?pez-Perrote A,Casta?o R,Melero R,Zamarro T,Kurosawa H,Ohnishi T,Uchiyama A,Aoyagi K,Buchwald G,Kataoka N,Yamashita A,Llorca O

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.
Structure (London, England : 1993)22(8)1105-1119(2014 Aug)
Melero R,Uchiyama A,Casta?o R,Kataoka N,Kurosawa H,Ohno S,Yamashita A,Llorca O

Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Nucleic acids research42(4)2673-86(2014 Feb)
Clerici M,Deniaud A,Boehm V,Gehring NH,Schaffitzel C,Cusack S

Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells.
International journal of oncology44(1)222-8(2014 Jan)
Zhao X,Nogawa A,Matsunaga T,Takegami T,Nakagawa H,Ishigaki Y

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UPF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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