HIGD1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIGD1A
Entrez Gene ID	25994
Full Name	HIG1 hypoxia inducible domain family member 1A
Synonyms	HIG1,RCF1a
General protein information	Preferred Names HIG1 hypoxia inducible domain family member 1A Names HIG1 domain family member 1A, mitochondrial HIG1 domain family, member 1A RCF1 homolog A hypoxia inducible gene 1 hypoxia-inducible gene 1 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p22.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099669.1	NP_001093139.1	HIG1 domain family member 1A, mitochondrial isoform b
NM_014056.3	NP_054775.2	HIG1 domain family member 1A, mitochondrial isoform b
NM_001099668.1	NP_001093138.1	HIG1 domain family member 1A, mitochondrial isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC101902290	XP_005205509.1
Bos taurus (cattle)	LOC788501	XP_003587982.1
Xenopus tropicalis (tropical clawed frog)	higd1a	NP_001011058.1
	HIGD1A	XP_001115209.2
Canis lupus familiaris (dog)	LOC100688295	XP_005633366.1
Bos taurus (cattle)	LOC101907084	XP_005212880.1
Bos taurus (cattle)	LOC101904447	XP_005202576.1
Bos taurus (cattle)	HIGD1A	XP_005228004.1
Bos taurus (cattle)	LOC101907436	XP_005218919.1
Mus musculus (house mouse)	Higd1a	NP_062788.1
Gallus gallus (chicken)	HIGD1A	NP_001026128.1
Danio rerio (zebrafish)	hig1	NP_956394.1
Homo sapiens (human)	HIGD1A	NP_001093138.1
Pan troglodytes (chimpanzee)	HIGD1A	XP_003309789.1
Canis lupus familiaris (dog)	HIGD1A	XP_005634369.1
Bos taurus (cattle)	LOC100337355	XP_005224108.1
Rattus norvegicus (Norway rat)	Higd1a	NP_543178.2
Bos taurus (cattle)	LOC101902273	XP_005210901.1
Bos taurus (cattle)	HIGD1D	NP_001070582.1

Gene Ontology
Bibliography

Related articles in PubMed

Higd1a is a positive regulator of cytochrome c oxidase.
Hayashi T, Asano Y, Shintani Y, Aoyama H, Kioka H, Tsukamoto O, Hikita M, Shinzawa-Itoh K, Takafuji K, Higo S, Kato H, Yamazaki S, Matsuoka K, Nakano A, Asanuma H, Asakura M, Minamino T, Goto Y, Ogura T, Kitakaze M, Komuro I, Sakata Y, Tsukihara T, Yoshikawa S, Takashima S
Proceedings of the National Academy of Sciences of the United States of America112(5)1553-8(2015 Feb)

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Goldstein JI, Jarskog LF, Hilliard C, Alfirevic A, Duncan L, Fourches D, Huang H, Lek M, Neale BM, Ripke S, Shianna K, Szatkiewicz JP, Tropsha A, van den Oord EJ, Cascorbi I, Dettling M, Gazit E, Goff DC, Holden AL, Kelly DL, Malhotra AK, Nielsen J, Pirmohamed M, Rujescu D, Werge T, Levy DL, Josiassen RC, Kennedy JL, Lieberman JA, Daly MJ, Sullivan PF
Nature communications54757(2014 Sep)

Higd-1a interacts with Opa1 and is required for the morphological and functional integrity of mitochondria.
An HJ, Cho G, Lee JO, Paik SG, Kim YS, Lee H
Proceedings of the National Academy of Sciences of the United States of America110(32)13014-9(2013 Aug)

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Ameri K, Rajah AM, Nguyen V, Sanders TA, Jahangiri A, Delay M, Donne M, Choi HJ, Tormos KV, Yeghiazarians Y, Jeffrey SS, Rinaudo PF, Rowitch DH, Aghi M, Maltepe E
PloS one8(4)e62758(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that hypoxia-induced gene domain protein-1a (Higd-1a) inhibits Optic atrophy 1 (Opa1) cleavage and is required for mitochondrial fusion by virtue of its interaction with Opa1.
Title: Higd-1a interacts with Opa1 and is required for the morphological and functional integrity of mitochondria.

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Title: Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.

depletion of HIG1 increased gamma-secretase activation and enhanced hypoxia-induced mitochondrial dysfunction
Title: HIG1, a novel regulator of mitochondrial ?-secretase, maintains normal mitochondrial function.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following HIGD1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIGD1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08867	NM_001099669.1 Latest version!	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08867	NM_014056.3 Latest version!	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08862	NM_001099668.1 Latest version!	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIGD1A ( NM_001099669.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093139.1 Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08867
Clone ID Related Accession (Same CDS sequence)	NM_001099669.1 , NM_014056.3
Accession Version	NM_001099669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	HIG1 domain family member 1A, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG708126.1, BP370118.1, BC070277.1, BM983580.1, AC099329.2 and BM983292.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and a protein (isoform b) with a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.89862.1, SRR1163658.15250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAACAG ACACAGGTGT TTCCCTTCCT TCATATGAGG AAGATCAGGG ATCAAAACTC 
ATTCGAAAAG CTAAAGAGGC ACCATTCGTA CCCGTTGGAA TAGCGGGTTT TGCAGCAATT 
GTTGCATATG GATTATATAA ACTGAAGAGC AGGGGAAATA CTAAAATGTC CATTCATCTG 
ATCCACATGC GTGTGGCAGC CCAAGGCTTT GTTGTAGGAG CAATGACTGT TGGTATGGGC 
TATTCCATGT ATCGGGAATT CTGGGCAAAA CCTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093139.1
CDS	147..428
Translation	MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

Target ORF information:

RefSeq Version	NM_001099669.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099669.1

ORF Insert Sequence:

ATGTCAACAG ACACAGGTGT TTCCCTTCCT TCATATGAGG AAGATCAGGG ATCAAAACTC 
ATTCGAAAAG CTAAAGAGGC ACCATTCGTA CCCGTTGGAA TAGCGGGTTT TGCAGCAATT 
GTTGCATATG GATTATATAA ACTGAAGAGC AGGGGAAATA CTAAAATGTC CATTCATCTG 
ATCCACATGC GTGTGGCAGC CCAAGGCTTT GTTGTAGGAG CAATGACTGT TGGTATGGGC 
TATTCCATGT ATCGGGAATT CTGGGCAAAA CCTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIGD1A ( NM_014056.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054775.2 Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08867
Clone ID Related Accession (Same CDS sequence)	NM_001099669.1 , NM_014056.3
Accession Version	NM_014056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	HIG1 domain family member 1A, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG708126.1, BC070277.1, BM983580.1, AC099329.2 and BM983292.1. On Jul 24, 2007 this sequence version replaced NM_014056.2. Transcript Variant: This variant (3) contains a distinct 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and a protein (isoform b) with a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225571.1, BC070277.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAACAG ACACAGGTGT TTCCCTTCCT TCATATGAGG AAGATCAGGG ATCAAAACTC 
ATTCGAAAAG CTAAAGAGGC ACCATTCGTA CCCGTTGGAA TAGCGGGTTT TGCAGCAATT 
GTTGCATATG GATTATATAA ACTGAAGAGC AGGGGAAATA CTAAAATGTC CATTCATCTG 
ATCCACATGC GTGTGGCAGC CCAAGGCTTT GTTGTAGGAG CAATGACTGT TGGTATGGGC 
TATTCCATGT ATCGGGAATT CTGGGCAAAA CCTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054775.2
CDS	123..404
Translation	MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

Target ORF information:

RefSeq Version	NM_014056.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014056.3

ORF Insert Sequence:

ATGTCAACAG ACACAGGTGT TTCCCTTCCT TCATATGAGG AAGATCAGGG ATCAAAACTC 
ATTCGAAAAG CTAAAGAGGC ACCATTCGTA CCCGTTGGAA TAGCGGGTTT TGCAGCAATT 
GTTGCATATG GATTATATAA ACTGAAGAGC AGGGGAAATA CTAAAATGTC CATTCATCTG 
ATCCACATGC GTGTGGCAGC CCAAGGCTTT GTTGTAGGAG CAATGACTGT TGGTATGGGC 
TATTCCATGT ATCGGGAATT CTGGGCAAAA CCTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIGD1A ( NM_001099668.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093138.1 Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08862
Clone ID Related Accession (Same CDS sequence)	NM_001099668.1
Accession Version	NM_001099668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	HIG1 domain family member 1A, mitochondrial isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP208784.1, DA428926.1, BC070277.1, BM983580.1, AC099329.2 and BM983292.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1165066.1, BP208784.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGA AGCTTGTGGA GGAGATTCTT CAAGCAATCA CTATGTCAAC AGACACAGGT 
GTTTCCCTTC CTTCATATGA GGAAGATCAG GGATCAAAAC TCATTCGAAA AGCTAAAGAG 
GCACCATTCG TACCCGTTGG AATAGCGGGT TTTGCAGCAA TTGTTGCATA TGGATTATAT 
AAACTGAAGA GCAGGGGAAA TACTAAAATG TCCATTCATC TGATCCACAT GCGTGTGGCA 
GCCCAAGGCT TTGTTGTAGG AGCAATGACT GTTGGTATGG GCTATTCCAT GTATCGGGAA 
TTCTGGGCAA AACCTAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093138.1
CDS	252..575
Translation	MEQKLVEEILQAITMSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

Target ORF information:

RefSeq Version	NM_001099668.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HIG1 hypoxia inducible domain family member 1A (HIGD1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099668.1

ORF Insert Sequence:

ATGGAGCAGA AGCTTGTGGA GGAGATTCTT CAAGCAATCA CTATGTCAAC AGACACAGGT 
GTTTCCCTTC CTTCATATGA GGAAGATCAG GGATCAAAAC TCATTCGAAA AGCTAAAGAG 
GCACCATTCG TACCCGTTGG AATAGCGGGT TTTGCAGCAA TTGTTGCATA TGGATTATAT 
AAACTGAAGA GCAGGGGAAA TACTAAAATG TCCATTCATC TGATCCACAT GCGTGTGGCA 
GCCCAAGGCT TTGTTGTAGG AGCAATGACT GTTGGTATGG GCTATTCCAT GTATCGGGAA 
TTCTGGGCAA AACCTAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Higd1a is a positive regulator of cytochrome c oxidase.
Proceedings of the National Academy of Sciences of the United States of America112(5)1553-8(2015 Feb)
Hayashi T,Asano Y,Shintani Y,Aoyama H,Kioka H,Tsukamoto O,Hikita M,Shinzawa-Itoh K,Takafuji K,Higo S,Kato H,Yamazaki S,Matsuoka K,Nakano A,Asanuma H,Asakura M,Minamino T,Goto Y,Ogura T,Kitakaze M,Komuro I,Sakata Y,Tsukihara T,Yoshikawa S,Takashima S

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Nature communications54757(2014 Sep)
Goldstein JI,Jarskog LF,Hilliard C,Alfirevic A,Duncan L,Fourches D,Huang H,Lek M,Neale BM,Ripke S,Shianna K,Szatkiewicz JP,Tropsha A,van den Oord EJ,Cascorbi I,Dettling M,Gazit E,Goff DC,Holden AL,Kelly DL,Malhotra AK,Nielsen J,Pirmohamed M,Rujescu D,Werge T,Levy DL,Josiassen RC,Kennedy JL,Lieberman JA,Daly MJ,Sullivan PF

Higd-1a interacts with Opa1 and is required for the morphological and functional integrity of mitochondria.
Proceedings of the National Academy of Sciences of the United States of America110(32)13014-9(2013 Aug)
An HJ,Cho G,Lee JO,Paik SG,Kim YS,Lee H

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
PloS one8(4)e62758(2013)
Ameri K,Rajah AM,Nguyen V,Sanders TA,Jahangiri A,Delay M,Donne M,Choi HJ,Tormos KV,Yeghiazarians Y,Jeffrey SS,Rinaudo PF,Rowitch DH,Aghi M,Maltepe E

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HIGD1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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