NECAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAP1
Entrez Gene ID	25977
Full Name	NECAP endocytosis associated 1
Synonyms	EIEE21
General protein information	Preferred Names NECAP endocytosis associated 1 Names adaptin ear-binding coat-associated protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	This gene encodes a protein containing two characteristic WXXF motifs. The encoded protein localizes to clathrin-coated vesicles, where it binds components of the adapter protein complexes and aids in endocytosis. Loss of function of this gene results in early infantile epileptic encephalopathy-21. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014].
Disorder MIM:	611623
Disorder Html:	?Epileptic encephalopathy, early infantile, 21, 615833 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015509.4	NP_056324.2	adaptin ear-binding coat-associated protein 1
NM_015509.3	NP_056324.2	adaptin ear-binding coat-associated protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NECAP1	XP_508992.2
Rattus norvegicus (Norway rat)	Necap1	NP_001025090.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000322	XP_310797.5
Danio rerio (zebrafish)	necap1	NP_957016.1
Bos taurus (cattle)	NECAP1	NP_001029383.1
Mus musculus (house mouse)	Necap1	NP_080543.2
Gallus gallus (chicken)	NECAP1	XP_003640487.1
Homo sapiens (human)	NECAP1	NP_056324.2
Canis lupus familiaris (dog)	NECAP1	XP_543832.2
	NECAP1	XP_001113344.1
Drosophila melanogaster (fruit fly)	CG9132	NP_996490.1

Gene Ontology
Bibliography

Related articles in PubMed

NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.
Alazami AM, Hijazi H, Kentab AY, Alkuraya FS
Journal of medical genetics51(4)224-8(2014 Apr)

Characterization of the localization and function of NECAP 1 in neurons.
Murshid A, Srivastava A, Kumar R, Presley JF
Journal of neurochemistry98(6)1746-62(2006 Sep)

Molecular mechanisms in clathrin-mediated membrane budding revealed through subcellular proteomics.
Ritter B, Blondeau F, Denisov AY, Gehring K, McPherson PS
Biochemical Society transactions32(Pt 5)769-73(2004 Nov)

Dual engagement regulation of protein interactions with the AP-2 adaptor alpha appendage.
Mishra SK, Hawryluk MJ, Brett TJ, Keyel PA, Dupin AL, Jha A, Heuser JE, Fremont DH, Traub LM
The Journal of biological chemistry279(44)46191-203(2004 Oct)

Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif.
Ritter B, Philie J, Girard M, Tung EC, Blondeau F, McPherson PS
EMBO reports4(11)1089-95(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NECAP1 mutation links trafficking pathway in early infantile epileptic encephalopathy.
Title: NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The following NECAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27985	NM_015509.4 Latest version!	Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu27985	NM_015509.3	Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAP1 ( NM_015509.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056324.2 Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27985
Clone ID Related Accession (Same CDS sequence)	NM_015509.4 , NM_015509.3
Accession Version	NM_015509.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	adaptin ear-binding coat-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006511.5. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_015509.3. Transcript Variant: This variant (1) encodes a functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.269232.1, SRR1660805.153748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000339754.11/ ENSP00000341737.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACCG AGTTGGAGTA CGAGTCTGTG CTGTGTGTGA AGCCAGACGT CAGCGTCTAC 
CGGATTCCGC CCCGGGCCTC CAACCGCGGT TACAGGGCCT CTGACTGGAA ATTAGACCAG 
CCTGATTGGA CTGGTCGCCT CCGAATCACT TCAAAAGGGA AGACTGCCTA TATCAAACTC 
GAGGATAAAG TTTCAGGGGA GCTCTTTGCT CAGGCACCAG TAGAACAATA TCCTGGTATT 
GCTGTGGAGA CGGTGACAGA TTCTAGCCGC TACTTTGTAA TCCGGATCCA GGATGGTACT 
GGGCGCAGTG CTTTCATTGG CATTGGCTTC ACAGATCGGG GAGATGCCTT CGACTTTAAT 
GTCTCCTTGC AGGATCACTT CAAGTGGGTA AAGCAGGAAT CTGAGATTTC CAAGGAATCT 
CAAGAAATGG ATGCTCGTCC TAAGTTGGAT CTGGGCTTCA AGGAAGGACA AACCATCAAG 
TTGTGTATCG GGAACATTAC AAACAAGAAA GGAGGTGCTT CTAAGCCCAG GACTGCAAGG 
GGTGGGGGTC TGAGCTTACT CCCACCCCCG CCAGGAGGCA AAGTCACTAT TCCCCCACCA 
TCCTCCTCAG TTGCCATCAG CAATCATGTC ACCCCACCAC CCATTCCGAA ATCTAACCAT 
GGAGGCAGTG ATGCAGATAT CCTTTTAGAT TTGGATTCTC CTGCTCCTGT CACGACACCA 
GCACCAACTC CAGTTTCTGT AAGCAATGAC TTGTGGGGAG ACTTCAGCAC TGCCTCCAGC 
TCTGTTCCAA ACCAGGCACC ACAGCCATCC AACTGGGTCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056324.2
CDS	16..843
Translation	MATELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKTAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLCIGNITNKKGGASKPRTARGGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGSDADILLDLDSPAPVTTPAPTPVSVSNDLWGDFSTASSSVPNQAPQPSNWVQF

Target ORF information:

RefSeq Version	NM_015509.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015509.4

ORF Insert Sequence:

ATGGCGACCG AGTTGGAGTA CGAGTCTGTG CTGTGTGTGA AGCCAGACGT CAGCGTCTAC 
CGGATTCCGC CCCGGGCCTC CAACCGCGGT TACAGGGCCT CTGACTGGAA ATTAGACCAG 
CCTGATTGGA CTGGTCGCCT CCGAATCACT TCAAAAGGGA AGACTGCCTA TATCAAACTC 
GAGGATAAAG TTTCAGGGGA GCTCTTTGCT CAGGCACCAG TAGAACAATA TCCTGGTATT 
GCTGTGGAGA CGGTGACAGA TTCTAGCCGC TACTTTGTAA TCCGGATCCA GGATGGTACT 
GGGCGCAGTG CTTTCATTGG CATTGGCTTC ACAGATCGGG GAGATGCCTT CGACTTTAAT 
GTCTCCTTGC AGGATCACTT CAAGTGGGTA AAGCAGGAAT CTGAGATTTC CAAGGAATCT 
CAAGAAATGG ATGCTCGTCC TAAGTTGGAT CTGGGCTTCA AGGAAGGACA AACCATCAAG 
TTGTGTATCG GGAACATTAC AAACAAGAAA GGAGGTGCTT CTAAGCCCAG GACTGCAAGG 
GGTGGGGGTC TGAGCTTACT CCCACCCCCG CCAGGAGGCA AAGTCACTAT TCCCCCACCA 
TCCTCCTCAG TTGCCATCAG CAATCATGTC ACCCCACCAC CCATTCCGAA ATCTAACCAT 
GGAGGCAGTG ATGCAGATAT CCTTTTAGAT TTGGATTCTC CTGCTCCTGT CACGACACCA 
GCACCAACTC CAGTTTCTGT AAGCAATGAC TTGTGGGGAG ACTTCAGCAC TGCCTCCAGC 
TCTGTTCCAA ACCAGGCACC ACAGCCATCC AACTGGGTCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAP1 ( NM_015509.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056324.2 Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27985
Clone ID Related Accession (Same CDS sequence)	NM_015509.4 , NM_015509.3
Accession Version	NM_015509.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	adaptin ear-binding coat-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA496321.1, BC110876.1 and AI688397.1. This sequence is a reference standard in the RefSeqGene project. On Oct 28, 2008 this sequence version replaced NM_015509.2. Transcript Variant: This variant (1) encodes a functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.114642.1, AK074880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCG AGTTGGAGTA CGAGTCTGTG CTGTGTGTGA AGCCAGACGT CAGCGTCTAC 
CGGATTCCGC CCCGGGCCTC CAACCGCGGT TACAGGGCCT CTGACTGGAA ATTAGACCAG 
CCTGATTGGA CTGGTCGCCT CCGAATCACT TCAAAAGGGA AGACTGCCTA TATCAAACTC 
GAGGATAAAG TTTCAGGGGA GCTCTTTGCT CAGGCACCAG TAGAACAATA TCCTGGTATT 
GCTGTGGAGA CGGTGACAGA TTCTAGCCGC TACTTTGTAA TCCGGATCCA GGATGGTACT 
GGGCGCAGTG CTTTCATTGG CATTGGCTTC ACAGATCGGG GAGATGCCTT CGACTTTAAT 
GTCTCCTTGC AGGATCACTT CAAGTGGGTA AAGCAGGAAT CTGAGATTTC CAAGGAATCT 
CAAGAAATGG ATGCTCGTCC TAAGTTGGAT CTGGGCTTCA AGGAAGGACA AACCATCAAG 
TTGTGTATCG GGAACATTAC AAACAAGAAA GGAGGTGCTT CTAAGCCCAG GACTGCAAGG 
GGTGGGGGTC TGAGCTTACT CCCACCCCCG CCAGGAGGCA AAGTCACTAT TCCCCCACCA 
TCCTCCTCAG TTGCCATCAG CAATCATGTC ACCCCACCAC CCATTCCGAA ATCTAACCAT 
GGAGGCAGTG ATGCAGATAT CCTTTTAGAT TTGGATTCTC CTGCTCCTGT CACGACACCA 
GCACCAACTC CAGTTTCTGT AAGCAATGAC TTGTGGGGAG ACTTCAGCAC TGCCTCCAGC 
TCTGTTCCAA ACCAGGCACC ACAGCCATCC AACTGGGTCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056324.2
CDS	79..906
Translation	MATELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKTAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLCIGNITNKKGGASKPRTARGGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGSDADILLDLDSPAPVTTPAPTPVSVSNDLWGDFSTASSSVPNQAPQPSNWVQF

Target ORF information:

RefSeq Version	NM_015509.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NECAP endocytosis associated 1 (NECAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015509.3

ORF Insert Sequence:

ATGGCGACCG AGTTGGAGTA CGAGTCTGTG CTGTGTGTGA AGCCAGACGT CAGCGTCTAC 
CGGATTCCGC CCCGGGCCTC CAACCGCGGT TACAGGGCCT CTGACTGGAA ATTAGACCAG 
CCTGATTGGA CTGGTCGCCT CCGAATCACT TCAAAAGGGA AGACTGCCTA TATCAAACTC 
GAGGATAAAG TTTCAGGGGA GCTCTTTGCT CAGGCACCAG TAGAACAATA TCCTGGTATT 
GCTGTGGAGA CGGTGACAGA TTCTAGCCGC TACTTTGTAA TCCGGATCCA GGATGGTACT 
GGGCGCAGTG CTTTCATTGG CATTGGCTTC ACAGATCGGG GAGATGCCTT CGACTTTAAT 
GTCTCCTTGC AGGATCACTT CAAGTGGGTA AAGCAGGAAT CTGAGATTTC CAAGGAATCT 
CAAGAAATGG ATGCTCGTCC TAAGTTGGAT CTGGGCTTCA AGGAAGGACA AACCATCAAG 
TTGTGTATCG GGAACATTAC AAACAAGAAA GGAGGTGCTT CTAAGCCCAG GACTGCAAGG 
GGTGGGGGTC TGAGCTTACT CCCACCCCCG CCAGGAGGCA AAGTCACTAT TCCCCCACCA 
TCCTCCTCAG TTGCCATCAG CAATCATGTC ACCCCACCAC CCATTCCGAA ATCTAACCAT 
GGAGGCAGTG ATGCAGATAT CCTTTTAGAT TTGGATTCTC CTGCTCCTGT CACGACACCA 
GCACCAACTC CAGTTTCTGT AAGCAATGAC TTGTGGGGAG ACTTCAGCAC TGCCTCCAGC 
TCTGTTCCAA ACCAGGCACC ACAGCCATCC AACTGGGTCC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.
Journal of medical genetics51(4)224-8(2014 Apr)
Alazami AM,Hijazi H,Kentab AY,Alkuraya FS

Characterization of the localization and function of NECAP 1 in neurons.
Journal of neurochemistry98(6)1746-62(2006 Sep)
Murshid A,Srivastava A,Kumar R,Presley JF

Molecular mechanisms in clathrin-mediated membrane budding revealed through subcellular proteomics.
Biochemical Society transactions32(Pt 5)769-73(2004 Nov)
Ritter B,Blondeau F,Denisov AY,Gehring K,McPherson PS

Dual engagement regulation of protein interactions with the AP-2 adaptor alpha appendage.
The Journal of biological chemistry279(44)46191-203(2004 Oct)
Mishra SK,Hawryluk MJ,Brett TJ,Keyel PA,Dupin AL,Jha A,Heuser JE,Fremont DH,Traub LM

Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif.
EMBO reports4(11)1089-95(2003 Nov)
Ritter B,Philie J,Girard M,Tung EC,Blondeau F,McPherson PS

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NECAP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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