UNC50 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UNC50
Entrez Gene ID	25972
Full Name	unc-50 inner nuclear membrane RNA binding protein
Synonyms	GMH1,HSD23,PDLs22,UNCL,URP
General protein information	Preferred Names unc-50 inner nuclear membrane RNA binding protein Names protein unc-50 homolog geal-6 membrane-associated high-copy suppressor 1 periodontal ligament-specific protein 22 protein GMH1 homolog unc-50 related unc-50-like protein uncoordinated-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Disorder MIM:	617826

mRNA and Protein(s)

mRNA	Protein	Name
NM_014044.5	NP_054763.2	protein unc-50 homolog
NM_001330353.1	NP_001317282.1	protein unc-50 homolog isoform 2
NM_014044.6	NP_054763.2	protein unc-50 homolog isoform 3
NM_001330353.2	NP_001317282.1	protein unc-50 homolog isoform 2
XM_006712403.3	XP_006712466.1	protein unc-50 homolog isoform X1
NM_001330354.1	NP_001317283.1	protein unc-50 homolog isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UNC50	XP_001158336.1
Bos taurus (cattle)	UNC50	NP_001014921.1
Danio rerio (zebrafish)	unc50	NP_956541.1
Kluyveromyces lactis	KLLA0F21230g	XP_456033.1
Schizosaccharomyces pombe (fission yeast)	mug16	NP_596353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002901	XP_312002.3
Arabidopsis thaliana (thale cress)	AT2G15240	NP_565370.1
Xenopus tropicalis (tropical clawed frog)	unc50	NP_001135705.1
Mus musculus (house mouse)	Unc50	NP_080399.1
Rattus norvegicus (Norway rat)	Unc50	NP_620274.1
Eremothecium gossypii	AGOS_AFR073C	NP_985620.1
Oryza sativa (rice)	Os09g0109500	NP_001062566.1
	UNC50	XP_001103333.1
Caenorhabditis elegans (roundworm)	unc-50	NP_499279.2
Saccharomyces cerevisiae (baker's yeast)	GMH1	NP_012955.1
Homo sapiens (human)	UNC50	NP_054763.2
Canis lupus familiaris (dog)	UNC50	XP_005626096.1
Gallus gallus (chicken)	UNC50	NP_001264682.1
Drosophila melanogaster (fruit fly)	CG9773	NP_649813.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga toxin 2 trafficking.
Selyunin AS, Iles LR, Bartholomeusz G, Mukhopadhyay S
The Journal of cell biology216(10)3249-3262(2017 Oct)

Biallelic mutation of UNC50, encoding a protein involved in AChR trafficking, is responsible for arthrogryposis.
Abiusi E, D'Alessandro M, Dieterich K, Quevarec L, Turczynski S, Valfort AC, Mezin P, Jouk PS, Gut M, Gut I, Bessereau JL, Melki J
Human molecular genetics26(20)3989-3994(2017 Oct)

UNC50 prompts G1/S transition and proliferation in HCC by regulation of epidermal growth factor receptor trafficking.
Fang Z, Zhou L, Jiang S, Cao L, Yu L
PloS one10(3)e0119338(2015)

Expression of UNCL during development of periodontal tissue and response of periodontal ligament fibroblasts to mechanical stress in vivo and in vitro.
Kim HJ, Choi YS, Jeong MJ, Kim BO, Lim SH, Kim DK, Kim CK, Park JC
Cell and tissue research327(1)25-31(2007 Jan)

UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein.
Fitzgerald J, Kennedy D, Viseshakul N, Cohen BN, Mattick J, Bateman JF, Forsayeth JR
Brain research877(1)110-23(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

depletion of UNC50 blocked early endosome-to-Golgi trafficking and induced lysosomal degradation of STx2. UNC50 acted by recruiting GBF1, an ADP ribosylation factor-guanine nucleotide exchange factor (ARF-GEF), to the Golgi.
Title: Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga toxin 2 trafficking.

UNC50 may plays some roles in HCC progression by affecting the EGFR pathway
Title: UNC50 prompts G1/S transition and proliferation in HCC by regulation of epidermal growth factor receptor trafficking.

UNCL plays important roles in the development, differentiation, and maintenance of periodontal tissues and in the mechanotransduction of periodontal ligament fibroblasts.
Title: Expression of UNCL during development of periodontal tissue and response of periodontal ligament fibroblasts to mechanical stress in vivo and in vitro.

The following UNC50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNC50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31433	NM_014044.6 Latest version!	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu36076	NM_001330354.1 Latest version!	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu36077	NM_001330353.2 Latest version!	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu36075	XM_006712403.3 Latest version!	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu31433	NM_014044.5	Homo sapiens unc-50 homolog (C. elegans) (UNC50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu36077	NM_001330353.1	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UNC50 ( NM_014044.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054763.2 Homo sapiens unc-50 homolog (C. elegans) (UNC50), mRNA.

Price & Availability↑

CloneID	OHu31433
Clone ID Related Accession (Same CDS sequence)	NM_014044.5 , NM_014044.6
Accession Version	NM_014044.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	protein unc-50 homolog
Comment

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGCATTGCC ATTTTTGAAA 
AATACAGTAA TTCTTCTGTA TCCATTTGCA CCTCTGATTC TGCTCTACGG GCTTTCCCTG 
GCACTGGGAT GGAACTTCAC CCATACTCTC TGTTCTTTCT ATAAGTACAG AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054763.2
Translation	MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPFLKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK

Target ORF information:

RefSeq Version	NM_014044.5
Organism	Homo sapiens(Human)
Definition	Homo sapiens unc-50 homolog (C. elegans) (UNC50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014044.5

ORF Insert Sequence:

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGCATTGCC ATTTTTGAAA 
AATACAGTAA TTCTTCTGTA TCCATTTGCA CCTCTGATTC TGCTCTACGG GCTTTCCCTG 
GCACTGGGAT GGAACTTCAC CCATACTCTC TGTTCTTTCT ATAAGTACAG AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC50 ( NM_014044.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_054763.2 Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu31433
Clone ID Related Accession (Same CDS sequence)	NM_014044.5 , NM_014044.6
Accession Version	NM_014044.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein unc-50 homolog isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010134.5, BC091481.1 and AK222599.1. On Aug 23, 2016 this sequence version replaced NM_014044.5. ##Evidence-Data-START## Transcript exon combination :: BC091481.1, AL080115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGCATTGCC ATTTTTGAAA 
AATACAGTAA TTCTTCTGTA TCCATTTGCA CCTCTGATTC TGCTCTACGG GCTTTCCCTG 
GCACTGGGAT GGAACTTCAC CCATACTCTC TGTTCTTTCT ATAAGTACAG AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054763.2
CDS	184..963
Translation	MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPFLKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK

Target ORF information:

RefSeq Version	NM_014044.6
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014044.6

ORF Insert Sequence:

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGCATTGCC ATTTTTGAAA 
AATACAGTAA TTCTTCTGTA TCCATTTGCA CCTCTGATTC TGCTCTACGG GCTTTCCCTG 
GCACTGGGAT GGAACTTCAC CCATACTCTC TGTTCTTTCT ATAAGTACAG AGTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC50 ( NM_001330354.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317283.1 Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu36076
Clone ID Related Accession (Same CDS sequence)	NM_001330354.1
Accession Version	NM_001330354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	protein unc-50 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010134.5 and AC064860.6. On Aug 23, 2016 this sequence version replaced XM_005263913.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.18715.1, SRR1163658.434407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCTTC AGAATACGTG TAAAAGAAAT GTTTTTCTTC CATCTAGGAA GATGTTACCG 
AGTACTTCAG TGAATTCCTT AGTGCAGGGG AACGGAGTCT TGAATTCCAG GGATGCGGCA 
AGACACACAG CCGGAGCGAA ACGCTACAAA TATCTGAGAA GGCTTTTCCG CTTTCGGCAA 
ATGGACTTTG AATTTGCTGC CTGGCAGATG CTCTACCTGT TCACATCCCC ACAGAGAGTT 
TACAGAAATT TTCATTATCG AAAACAGACG AAGGACCAGT GGGCCAGAGA TGACCCTGCT 
TTCTTGGTCC TGTTAAGTAT CTGGCTCTGT GTGTCCACTA TAGGATTTGG CTTTGTGCTG 
GACATGGGAT TCTTTGAGAC AATAAAGCTT CTCCTTTGGG TTGTACTCAT AGATTGTGTA 
GGCGTTGGTC TTCTGATAGC AACTTTAATG TGGTTCATCT CTAACAAGTA TTTAGTGAAA 
CGACAGAGCA GAGACTATGA TGTGGAATGG GGCTATGCTT TTGATGTGCA TCTCAATGCT 
TTTTATCCAC TCCTGGTCAT TTTGCATTTT ATCCAGCTTT TTTTCATCAA CCATGTTATC 
CTGACAGACA CATTTATTGG ATATTTAGTT GGAAATACCT TATGGTTGGT TGCAGTTGGC 
TATTATATCT ATGTAACTTT CCTGGGATAC AGTGCATTGC CATTTTTGAA AAATACAGTA 
ATTCTTCTGT ATCCATTTGC ACCTCTGATT CTGCTCTACG GGCTTTCCCT GGCACTGGGA 
TGGAACTTCA CCCATACTCT CTGTTCTTTC TATAAGTACA GAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317283.1
CDS	246..1076
Translation	MFLQNTCKRNVFLPSRKMLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPFLKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK

Target ORF information:

RefSeq Version	NM_001330354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330354.1

ORF Insert Sequence:

ATGTTTCTTC AGAATACGTG TAAAAGAAAT GTTTTTCTTC CATCTAGGAA GATGTTACCG 
AGTACTTCAG TGAATTCCTT AGTGCAGGGG AACGGAGTCT TGAATTCCAG GGATGCGGCA 
AGACACACAG CCGGAGCGAA ACGCTACAAA TATCTGAGAA GGCTTTTCCG CTTTCGGCAA 
ATGGACTTTG AATTTGCTGC CTGGCAGATG CTCTACCTGT TCACATCCCC ACAGAGAGTT 
TACAGAAATT TTCATTATCG AAAACAGACG AAGGACCAGT GGGCCAGAGA TGACCCTGCT 
TTCTTGGTCC TGTTAAGTAT CTGGCTCTGT GTGTCCACTA TAGGATTTGG CTTTGTGCTG 
GACATGGGAT TCTTTGAGAC AATAAAGCTT CTCCTTTGGG TTGTACTCAT AGATTGTGTA 
GGCGTTGGTC TTCTGATAGC AACTTTAATG TGGTTCATCT CTAACAAGTA TTTAGTGAAA 
CGACAGAGCA GAGACTATGA TGTGGAATGG GGCTATGCTT TTGATGTGCA TCTCAATGCT 
TTTTATCCAC TCCTGGTCAT TTTGCATTTT ATCCAGCTTT TTTTCATCAA CCATGTTATC 
CTGACAGACA CATTTATTGG ATATTTAGTT GGAAATACCT TATGGTTGGT TGCAGTTGGC 
TATTATATCT ATGTAACTTT CCTGGGATAC AGTGCATTGC CATTTTTGAA AAATACAGTA 
ATTCTTCTGT ATCCATTTGC ACCTCTGATT CTGCTCTACG GGCTTTCCCT GGCACTGGGA 
TGGAACTTCA CCCATACTCT CTGTTCTTTC TATAAGTACA GAGTGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC50 ( NM_001330353.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317282.1 Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu36077
Clone ID Related Accession (Same CDS sequence)	NM_001330353.1 , NM_001330353.2
Accession Version	NM_001330353.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	protein unc-50 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010134.5 and AC064860.6. On Aug 23, 2016 this sequence version replaced XM_006712404.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.423222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGTTTGGAT TCCTTTCTTT 
TCCAGCATTG CCATTTTTGA AAAATACAGT AATTCTTCTG TATCCATTTG CACCTCTGAT 
TCTGCTCTAC GGGCTTTCCC TGGCACTGGG ATGGAACTTC ACCCATACTC TCTGTTCTTT 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317282.1
CDS	184..969
Translation	MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSVWIPFFSSIAIFEKYSNSSVSICTSDSALRAFPGTGMELHPYSLFFL

Target ORF information:

RefSeq Version	NM_001330353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330353.1

ORF Insert Sequence:

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGTTTGGAT TCCTTTCTTT 
TCCAGCATTG CCATTTTTGA AAAATACAGT AATTCTTCTG TATCCATTTG CACCTCTGAT 
TCTGCTCTAC GGGCTTTCCC TGGCACTGGG ATGGAACTTC ACCCATACTC TCTGTTCTTT 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC50 ( NM_001330353.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317282.1 Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.

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CloneID	OHu36077
Clone ID Related Accession (Same CDS sequence)	NM_001330353.1 , NM_001330353.2
Accession Version	NM_001330353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	protein unc-50 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010134.5 and AC064860.6. On Jun 2, 2019 this sequence version replaced NM_001330353.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.423222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGTTTGGAT TCCTTTCTTT 
TCCAGCATTG CCATTTTTGA AAAATACAGT AATTCTTCTG TATCCATTTG CACCTCTGAT 
TCTGCTCTAC GGGCTTTCCC TGGCACTGGG ATGGAACTTC ACCCATACTC TCTGTTCTTT 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317282.1
CDS	143..928
Translation	MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSVWIPFFSSIAIFEKYSNSSVSICTSDSALRAFPGTGMELHPYSLFFL

Target ORF information:

RefSeq Version	NM_001330353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330353.2

ORF Insert Sequence:

ATGTTACCGA GTACTTCAGT GAATTCCTTA GTGCAGGGGA ACGGAGTCTT GAATTCCAGG 
GATGCGGCAA GACACACAGC CGGAGCGAAA CGCTACAAAT ATCTGAGAAG GCTTTTCCGC 
TTTCGGCAAA TGGACTTTGA ATTTGCTGCC TGGCAGATGC TCTACCTGTT CACATCCCCA 
CAGAGAGTTT ACAGAAATTT TCATTATCGA AAACAGACGA AGGACCAGTG GGCCAGAGAT 
GACCCTGCTT TCTTGGTCCT GTTAAGTATC TGGCTCTGTG TGTCCACTAT AGGATTTGGC 
TTTGTGCTGG ACATGGGATT CTTTGAGACA ATAAAGCTTC TCCTTTGGGT TGTACTCATA 
GATTGTGTAG GCGTTGGTCT TCTGATAGCA ACTTTAATGT GGTTCATCTC TAACAAGTAT 
TTAGTGAAAC GACAGAGCAG AGACTATGAT GTGGAATGGG GCTATGCTTT TGATGTGCAT 
CTCAATGCTT TTTATCCACT CCTGGTCATT TTGCATTTTA TCCAGCTTTT TTTCATCAAC 
CATGTTATCC TGACAGACAC ATTTATTGGA TATTTAGTTG GAAATACCTT ATGGTTGGTT 
GCAGTTGGCT ATTATATCTA TGTAACTTTC CTGGGATACA GTGTTTGGAT TCCTTTCTTT 
TCCAGCATTG CCATTTTTGA AAAATACAGT AATTCTTCTG TATCCATTTG CACCTCTGAT 
TCTGCTCTAC GGGCTTTCCC TGGCACTGGG ATGGAACTTC ACCCATACTC TCTGTTCTTT 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNC50 ( XM_006712403.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712466.1 Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant X1, mRNA.

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CloneID	OHu36075
Clone ID Related Accession (Same CDS sequence)	XM_006712403.3
Accession Version	XM_006712403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein unc-50 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTC AGAATACGTG TAAAAGAAAT GTTTTTCTTC CATCTAGGAA GATGTTACCG 
AGTACTTCAG TGAATTCCTT AGTGCAGGGG AACGGAGTCT TGAATTCCAG GGATGCGGCA 
AGACACACAG CCGGAGCGAA ACGCTACAAA TATCTGAGAA GGCTTTTCCG CTTTCGGCAA 
ATGGACTTTG AATTTGCTGC CTGGCAGATG CTCTACCTGT TCACATCCCC ACAGAGAGTT 
TACAGAAATT TTCATTATCG AAAACAGACG AAGGACCAGT GGGCCAGAGA TGACCCTGCT 
TTCTTGGTCC TGTTAAGTAT CTGGCTCTGT GTGTCCACTA TAGGATTTGG CTTTGTGCTG 
GACATGGGAT TCTTTGAGAC AATAAAGCTT CTCCTTTGGG TTGTACTCAT AGATTGTGTA 
GGCGTTGGTC TTCTGATAGC AACTTTAATG TGGTTCATCT CTAACAAGTA TTTAGTGAAA 
CGACAGAGCA GAGACTATGA TGTGGAATGG GGCTATGCTT TTGATGTGCA TCTCAATGCT 
TTTTATCCAC TCCTGGTCAT TTTGCATTTT ATCCAGCTTT TTTTCATCAA CCATGTTATC 
CTGACAGACA CATTTATTGG ATATTTAGTT GGAAATACCT TATGGTTGGT TGCAGTTGGC 
TATTATATCT ATGTAACTTT CCTGGGATAC AGTGTTTGGA TTCCTTTCTT TTCCAGCATT 
GCCATTTTTG AAAAATACAG TAATTCTTCT GTATCCATTT GCACCTCTGA TTCTGCTCTA 
CGGGCTTTCC CTGGCACTGG GATGGAACTT CACCCATACT CTCTGTTCTT TCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712466.1
CDS	198..1034
Translation	MFLQNTCKRNVFLPSRKMLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSVWIPFFSSIAIFEKYSNSSVSICTSDSALRAFPGTGMELHPYSLFFL

Target ORF information:

RefSeq Version	XM_006712403.3
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-50 inner nuclear membrane RNA binding protein (UNC50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712403.3

ORF Insert Sequence:

ATGTTTCTTC AGAATACGTG TAAAAGAAAT GTTTTTCTTC CATCTAGGAA GATGTTACCG 
AGTACTTCAG TGAATTCCTT AGTGCAGGGG AACGGAGTCT TGAATTCCAG GGATGCGGCA 
AGACACACAG CCGGAGCGAA ACGCTACAAA TATCTGAGAA GGCTTTTCCG CTTTCGGCAA 
ATGGACTTTG AATTTGCTGC CTGGCAGATG CTCTACCTGT TCACATCCCC ACAGAGAGTT 
TACAGAAATT TTCATTATCG AAAACAGACG AAGGACCAGT GGGCCAGAGA TGACCCTGCT 
TTCTTGGTCC TGTTAAGTAT CTGGCTCTGT GTGTCCACTA TAGGATTTGG CTTTGTGCTG 
GACATGGGAT TCTTTGAGAC AATAAAGCTT CTCCTTTGGG TTGTACTCAT AGATTGTGTA 
GGCGTTGGTC TTCTGATAGC AACTTTAATG TGGTTCATCT CTAACAAGTA TTTAGTGAAA 
CGACAGAGCA GAGACTATGA TGTGGAATGG GGCTATGCTT TTGATGTGCA TCTCAATGCT 
TTTTATCCAC TCCTGGTCAT TTTGCATTTT ATCCAGCTTT TTTTCATCAA CCATGTTATC 
CTGACAGACA CATTTATTGG ATATTTAGTT GGAAATACCT TATGGTTGGT TGCAGTTGGC 
TATTATATCT ATGTAACTTT CCTGGGATAC AGTGTTTGGA TTCCTTTCTT TTCCAGCATT 
GCCATTTTTG AAAAATACAG TAATTCTTCT GTATCCATTT GCACCTCTGA TTCTGCTCTA 
CGGGCTTTCC CTGGCACTGG GATGGAACTT CACCCATACT CTCTGTTCTT TCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga toxin 2 trafficking.
The Journal of cell biology216(10)3249-3262(2017 Oct)
Selyunin AS,Iles LR,Bartholomeusz G,Mukhopadhyay S

Biallelic mutation of UNC50, encoding a protein involved in AChR trafficking, is responsible for arthrogryposis.
Human molecular genetics26(20)3989-3994(2017 Oct)
Abiusi E,D'Alessandro M,Dieterich K,Quevarec L,Turczynski S,Valfort AC,Mezin P,Jouk PS,Gut M,Gut I,Bessereau JL,Melki J

UNC50 prompts G1/S transition and proliferation in HCC by regulation of epidermal growth factor receptor trafficking.
PloS one10(3)e0119338(2015)
Fang Z,Zhou L,Jiang S,Cao L,Yu L

Expression of UNCL during development of periodontal tissue and response of periodontal ligament fibroblasts to mechanical stress in vivo and in vitro.
Cell and tissue research327(1)25-31(2007 Jan)
Kim HJ,Choi YS,Jeong MJ,Kim BO,Lim SH,Kim DK,Kim CK,Park JC

UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein.
Brain research877(1)110-23(2000 Sep)
Fitzgerald J,Kennedy D,Viseshakul N,Cohen BN,Mattick J,Bateman JF,Forsayeth JR

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UNC50 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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