NDUFAF3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFAF3
Entrez Gene ID	25915
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 3
Synonyms	2P1,C3orf60,E3-3
General protein information	Preferred Names NADH:ubiquinone oxidoreductase complex assembly factor 3 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 NADH dehydrogenase (ubiquinone) complex I, assembly factor 3 nuclear protein E3-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2009].
Disorder MIM:	612911
Disorder Html:	Mitochondrial complex I deficiency, 252010 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199069.1	NP_951032.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a precursor
NM_199073.1	NP_951047.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
NM_199070.1	NP_951033.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
NM_199069.2	NP_951032.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a precursor
NM_199074.1	NP_951056.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDUFAF3	NP_951032.1
Rattus norvegicus (Norway rat)	Ndufaf3	NP_064465.1
Drosophila melanogaster (fruit fly)	CG5569	NP_611840.1
Pan troglodytes (chimpanzee)	NDUFAF3	XP_001162332.1
Canis lupus familiaris (dog)	NDUFAF3	XP_533836.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003819	XP_310380.2
Caenorhabditis elegans (roundworm)	nuaf-3	NP_001023329.1
	NDUFAF3	XP_001111675.1
Bos taurus (cattle)	NDUFAF3	NP_001039570.1
Mus musculus (house mouse)	Ndufaf3	NP_075736.1
Danio rerio (zebrafish)	ndufaf3	NP_001074084.1
Xenopus tropicalis (tropical clawed frog)	ndufaf3	NP_001005023.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ
Molecular cell63(4)621-632(2016 Aug)

TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex.
Guarani V, Paulo J, Zhai B, Huttlin EL, Gygi SP, Harper JW
Molecular and cellular biology34(5)847-61(2014 Mar)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

LC-MS/MS as an alternative for SDS-PAGE in blue native analysis of protein complexes.
Wessels HJ, Vogel RO, van den Heuvel L, Smeitink JA, Rodenburg RJ, Nijtmans LG, Farhoud MH
Proteomics9(17)4221-8(2009 Sep)

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
Saada A, Vogel RO, Hoefs SJ, van den Brand MA, Wessels HJ, Willems PH, Venselaar H, Shaag A, Barghuti F, Reish O, Shohat M, Huynen MA, Smeitink JA, van den Heuvel LP, Nijtmans LG
American journal of human genetics84(6)718-27(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
Title: Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.

Describes a similar transcript in mouse.
Title: 2P1, a novel male mouse cDNA specifically expressed during meiosis.

Describes a similar transcript in rat, and compares it to human and mouse transcripts.
Title: Molecular cloning of a novel alternatively spliced nuclear protein.

The following NDUFAF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFAF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29379	NM_199069.2 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu13118	NM_199073.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu13118	NM_199070.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu13118	NM_199074.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu29379	NM_199069.1	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFAF3 ( NM_199069.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_951032.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29379
Clone ID Related Accession (Same CDS sequence)	NM_199069.1 , NM_199069.2
Accession Version	NM_199069.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049955.1, BM559881.1, BC002873.2 and AA907148.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803614.223732.1, SRR1163657.261753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19463981; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCG CTCTCGCGCT ACGTAGCTTG TACCGAGCGC GACCCTCGCT GCGCTGTCCG 
CCCGTTGAGC TTCCCTGGGC CCCGCGGCGA GGGCATCGGC TCTCGCCGGC GGATGACGAG 
CTGTATCAGC GGACGCGCAT CTCTCTGCTG CAACGCGAGG CCGCTCAGGC AATGTACATC 
GACAGCTACA ACAGCCGCGG CTTCATGATA AACGGAAACC GCGTGCTCGG CCCCTGCGCT 
CTGCTCCCGC ACTCGGTGGT GCAGTGGAAC GTGGGATCCC ACCAGGACAT CACCGAAGAC 
AGCTTTTCCC TCTTCTGGTT GCTGGAGCCC CGGATAGAGA TCGTGGTGGT GGGGACTGGA 
GACCGGACCG AGAGGCTGCA GTCCCAGGTG CTTCAAGCCA TGAGGCAGCG GGGCATTGCT 
GTGGAAGTGC AGGACACGCC CAATGCCTGT GCCACCTTCA ACTTCCTGTG TCATGAAGGC 
CGAGTAACTG GAGCTGCTCT CATCCCTCCA CCAGGAGGGA CTTCACTTAC ATCTTTGGGC 
CAAGCTGCTC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951032.1
CDS	505..1059
Translation	MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

Target ORF information:

RefSeq Version	NM_199069.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199069.1

ORF Insert Sequence:

ATGGCCACCG CTCTCGCGCT ACGTAGCTTG TACCGAGCGC GACCCTCGCT GCGCTGTCCG 
CCCGTTGAGC TTCCCTGGGC CCCGCGGCGA GGGCATCGGC TCTCGCCGGC GGATGACGAG 
CTGTATCAGC GGACGCGCAT CTCTCTGCTG CAACGCGAGG CCGCTCAGGC AATGTACATC 
GACAGCTACA ACAGCCGCGG CTTCATGATA AACGGAAACC GCGTGCTCGG CCCCTGCGCT 
CTGCTCCCGC ACTCGGTGGT GCAGTGGAAC GTGGGATCCC ACCAGGACAT CACCGAAGAC 
AGCTTTTCCC TCTTCTGGTT GCTGGAGCCC CGGATAGAGA TCGTGGTGGT GGGGACTGGA 
GACCGGACCG AGAGGCTGCA GTCCCAGGTG CTTCAAGCCA TGAGGCAGCG GGGCATTGCT 
GTGGAAGTGC AGGACACGCC CAATGCCTGT GCCACCTTCA ACTTCCTGTG TCATGAAGGC 
CGAGTAACTG GAGCTGCTCT CATCCCTCCA CCAGGAGGGA CTTCACTTAC ATCTTTGGGC 
CAAGCTGCTC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF3 ( NM_199069.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_951032.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29379
Clone ID Related Accession (Same CDS sequence)	NM_199069.1 , NM_199069.2
Accession Version	NM_199069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002873.2 and AA907148.1. On May 17, 2019 this sequence version replaced NM_199069.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.313310.1, SRR1803616.320340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19463981; reported by MitoCarta MANE Ensembl match :: ENST00000326925.11/ ENSP00000323076.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCG CTCTCGCGCT ACGTAGCTTG TACCGAGCGC GACCCTCGCT GCGCTGTCCG 
CCCGTTGAGC TTCCCTGGGC CCCGCGGCGA GGGCATCGGC TCTCGCCGGC GGATGACGAG 
CTGTATCAGC GGACGCGCAT CTCTCTGCTG CAACGCGAGG CCGCTCAGGC AATGTACATC 
GACAGCTACA ACAGCCGCGG CTTCATGATA AACGGAAACC GCGTGCTCGG CCCCTGCGCT 
CTGCTCCCGC ACTCGGTGGT GCAGTGGAAC GTGGGATCCC ACCAGGACAT CACCGAAGAC 
AGCTTTTCCC TCTTCTGGTT GCTGGAGCCC CGGATAGAGA TCGTGGTGGT GGGGACTGGA 
GACCGGACCG AGAGGCTGCA GTCCCAGGTG CTTCAAGCCA TGAGGCAGCG GGGCATTGCT 
GTGGAAGTGC AGGACACGCC CAATGCCTGT GCCACCTTCA ACTTCCTGTG TCATGAAGGC 
CGAGTAACTG GAGCTGCTCT CATCCCTCCA CCAGGAGGGA CTTCACTTAC ATCTTTGGGC 
CAAGCTGCTC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951032.1
CDS	25..579
Translation	MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

Target ORF information:

RefSeq Version	NM_199069.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199069.2

ORF Insert Sequence:

ATGGCCACCG CTCTCGCGCT ACGTAGCTTG TACCGAGCGC GACCCTCGCT GCGCTGTCCG 
CCCGTTGAGC TTCCCTGGGC CCCGCGGCGA GGGCATCGGC TCTCGCCGGC GGATGACGAG 
CTGTATCAGC GGACGCGCAT CTCTCTGCTG CAACGCGAGG CCGCTCAGGC AATGTACATC 
GACAGCTACA ACAGCCGCGG CTTCATGATA AACGGAAACC GCGTGCTCGG CCCCTGCGCT 
CTGCTCCCGC ACTCGGTGGT GCAGTGGAAC GTGGGATCCC ACCAGGACAT CACCGAAGAC 
AGCTTTTCCC TCTTCTGGTT GCTGGAGCCC CGGATAGAGA TCGTGGTGGT GGGGACTGGA 
GACCGGACCG AGAGGCTGCA GTCCCAGGTG CTTCAAGCCA TGAGGCAGCG GGGCATTGCT 
GTGGAAGTGC AGGACACGCC CAATGCCTGT GCCACCTTCA ACTTCCTGTG TCATGAAGGC 
CGAGTAACTG GAGCTGCTCT CATCCCTCCA CCAGGAGGGA CTTCACTTAC ATCTTTGGGC 
CAAGCTGCTC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF3 ( NM_199073.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_951047.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13118
Clone ID Related Accession (Same CDS sequence)	NM_199073.1 , NM_199070.1 , NM_199074.1
Accession Version	NM_199073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD358942.1 and AA907148.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3 and 4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: BI825923.1, CD358942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19463981; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951047.1
CDS	310..693
Translation	MYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

Target ORF information:

RefSeq Version	NM_199073.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199073.1

ORF Insert Sequence:

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF3 ( NM_199070.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_951033.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13118
Clone ID Related Accession (Same CDS sequence)	NM_199073.1 , NM_199070.1 , NM_199074.1
Accession Version	NM_199070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049955.1 and AA907148.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3 and 4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: AL049955.1, CF994110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19463981; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951033.1
CDS	239..622
Translation	MYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

Target ORF information:

RefSeq Version	NM_199070.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199070.1

ORF Insert Sequence:

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFAF3 ( NM_199074.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_951056.1 Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13118
Clone ID Related Accession (Same CDS sequence)	NM_199073.1 , NM_199070.1 , NM_199074.1
Accession Version	NM_199074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ652614.1 and AA907148.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3 and 4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: BQ652614.1, BU594868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19463981; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951056.1
CDS	191..574
Translation	MYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ

Target ORF information:

RefSeq Version	NM_199074.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 3 (NDUFAF3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199074.1

ORF Insert Sequence:

ATGTACATCG ACAGCTACAA CAGCCGCGGC TTCATGATAA ACGGAAACCG CGTGCTCGGC 
CCCTGCGCTC TGCTCCCGCA CTCGGTGGTG CAGTGGAACG TGGGATCCCA CCAGGACATC 
ACCGAAGACA GCTTTTCCCT CTTCTGGTTG CTGGAGCCCC GGATAGAGAT CGTGGTGGTG 
GGGACTGGAG ACCGGACCGA GAGGCTGCAG TCCCAGGTGC TTCAAGCCAT GAGGCAGCGG 
GGCATTGCTG TGGAAGTGCA GGACACGCCC AATGCCTGTG CCACCTTCAA CTTCCTGTGT 
CATGAAGGCC GAGTAACTGG AGCTGCTCTC ATCCCTCCAC CAGGAGGGAC TTCACTTACA 
TCTTTGGGCC AAGCTGCTCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Molecular cell63(4)621-632(2016 Aug)
Floyd BJ,Wilkerson EM,Veling MT,Minogue CE,Xia C,Beebe ET,Wrobel RL,Cho H,Kremer LS,Alston CL,Gromek KA,Dolan BK,Ulbrich A,Stefely JA,Bohl SL,Werner KM,Jochem A,Westphall MS,Rensvold JW,Taylor RW,Prokisch H,Kim JP,Coon JJ,Pagliarini DJ

TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex.
Molecular and cellular biology34(5)847-61(2014 Mar)
Guarani V,Paulo J,Zhai B,Huttlin EL,Gygi SP,Harper JW

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

LC-MS/MS as an alternative for SDS-PAGE in blue native analysis of protein complexes.
Proteomics9(17)4221-8(2009 Sep)
Wessels HJ,Vogel RO,van den Heuvel L,Smeitink JA,Rodenburg RJ,Nijtmans LG,Farhoud MH

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
American journal of human genetics84(6)718-27(2009 Jun)
Saada A,Vogel RO,Hoefs SJ,van den Brand MA,Wessels HJ,Willems PH,Venselaar H,Shaag A,Barghuti F,Reish O,Shohat M,Huynen MA,Smeitink JA,van den Heuvel LP,Nijtmans LG

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NDUFAF3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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