AMBP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMBP
Entrez Gene ID	259
Full Name	alpha-1-microglobulin/bikunin precursor
Synonyms	A1M,EDC1,HCP,HI30,IATIL,ITI,ITIL,ITILC,UTI
General protein information	Preferred Names alpha-1-microglobulin/bikunin precursor Names protein AMBP bikunin complex-forming glycoprotein heterogeneous in charge growth-inhibiting protein 19 inter-alpha-trypsin inhibitor light chain protein HC trypstatin uristatin uronic-acid-rich protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q32
Summary	This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008].
Disorder MIM:	176870

mRNA and Protein(s)

mRNA	Protein	Name
NM_001633.3	NP_001624.1	protein AMBP preproprotein
NM_001633.4	NP_001624.1	protein AMBP preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	AMBP	NP_001624.1
Rattus norvegicus (Norway rat)	Ambp	NP_037033.1
Canis lupus familiaris (dog)	AMBP	XP_538807.3
Bos taurus (cattle)	AMBP	NP_776414.1
Danio rerio (zebrafish)	ambp	NP_957412.2
Pan troglodytes (chimpanzee)	AMBP	XP_001155186.2
Gallus gallus (chicken)	AMBP	NP_001264627.1
	AMBP	XP_002800088.1
Mus musculus (house mouse)	Ambp	NP_031469.1
Xenopus tropicalis (tropical clawed frog)	ambp	NP_989345.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

The crystal structure of bikunin from the inter-alpha-inhibitor complex: a serine protease inhibitor with two Kunitz domains.
Xu Y, Carr PD, Guss JM, Ollis DL
Journal of molecular biology276(5)955-66(1998 Mar)

The complete amino acid sequence of human complex-forming glycoprotein heterogeneous in charge (protein HC) from one individual.
L?pez Otin C, Grubb AO, M?ndez E
Archives of biochemistry and biophysics228(2)544-54(1984 Feb)

Complete amino acid sequence of human alpha 1-microglobulin.
Takagi T, Takagi K, Kawai T
Biochemical and biophysical research communications98(4)997-1001(1981 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There were significantly higher serum concentrations of fetal hemoglobin and alpha1-microglobulin and significantly lower first trimester serum concentrations of hemopexin in patients who later developed preeclampsia.
Title: Fetal hemoglobin, ?1-microglobulin and hemopexin are potential predictive first trimester biomarkers for preeclampsia.

Prophylactic UTI maintains the endothelial barrier function, increases VE-cadherin expression, and inhibits the phosphorylation of VE-cadherin at Tyr658 under inflammatory conditions
Title: Urinary trypsin inhibitor attenuates LPS-induced endothelial barrier dysfunction by upregulation of vascular endothelial-cadherin expression.

Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability; bikunin binds adjacent to the two heavy chains.
Title: The Compact and Biologically Relevant Structure of Inter-?-inhibitor Is Maintained by the Chondroitin Sulfate Chain and Divalent Cations.

Data indicate that two heme groups can be bound simultaneously at separate sites in the antioxidant alpha1-microglobulin.
Title: Structural and biochemical characterization of two heme binding sites on ?1-microglobulin using site directed mutagenesis and molecular simulation.

Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan.
Title: Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan.

The following AMBP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMBP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03642	NM_001633.4 Latest version!	Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu03642	NM_001633.3	Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMBP ( NM_001633.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001624.1 Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.

Price & Availability↑

CloneID	OHu03642
Clone ID Related Accession (Same CDS sequence)	NM_001633.3 , NM_001633.4
Accession Version	NM_001633.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	protein AMBP preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB141734.1 and BC041593.1. On May 11, 2008 this sequence version replaced NM_001633.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041593.1, ERR279833.5615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGAGCC TCGGGGCCCT GCTCTTGCTG CTGAGCGCCT GCCTGGCGGT GAGCGCTGGC 
CCTGTGCCAA CGCCGCCCGA CAACATCCAA GTGCAGGAAA ACTTCAATAT CTCTCGGATC 
TATGGGAAGT GGTACAACCT GGCCATCGGT TCCACCTGCC CCTGGCTGAA GAAGATCATG 
GACAGGATGA CAGTGAGCAC GCTGGTGCTG GGAGAGGGCG CTACAGAGGC GGAGATCAGC 
ATGACCAGCA CTCGTTGGCG GAAAGGTGTC TGTGAGGAGA CGTCTGGAGC TTATGAGAAA 
ACAGATACTG ATGGGAAGTT TCTCTATCAC AAATCCAAAT GGAACATAAC CATGGAGTCC 
TATGTGGTCC ACACCAACTA TGATGAGTAT GCCATTTTCC TGACCAAGAA ATTCAGCCGC 
CATCATGGAC CCACCATTAC TGCCAAGCTC TACGGGCGGG CGCCGCAGCT GAGGGAAACT 
CTCCTGCAGG ACTTCAGAGT GGTTGCCCAG GGTGTGGGCA TCCCTGAGGA CTCCATCTTC 
ACCATGGCTG ACCGAGGTGA ATGTGTCCCT GGGGAGCAGG AACCAGAGCC CATCTTAATC 
CCGAGAGTCC GGAGGGCTGT GCTACCCCAA GAAGAGGAAG GATCAGGGGG TGGGCAACTG 
GTAACTGAAG TCACCAAGAA AGAAGATTCC TGCCAGCTGG GCTACTCGGC CGGTCCCTGC 
ATGGGAATGA CCAGCAGGTA TTTCTATAAT GGTACATCCA TGGCCTGTGA GACTTTCCAG 
TACGGCGGCT GCATGGGCAA CGGTAACAAC TTCGTCACAG AAAAGGAGTG TCTGCAGACC 
TGCCGAACTG TGGCGGCCTG CAATCTCCCC ATAGTCCGGG GCCCCTGCCG AGCCTTCATC 
CAGCTCTGGG CATTTGATGC TGTCAAGGGG AAGTGCGTCC TCTTCCCCTA CGGGGGCTGC 
CAGGGCAACG GGAACAAGTT CTACTCAGAG AAGGAGTGCA GAGAGTACTG CGGTGTCCCT 
GGTGATGGTG ATGAGGAGCT GCTGCGCTTC TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001624.1
CDS	264..1322
Translation	MRSLGALLLLLSACLAVSAGPVPTPPDNIQVQENFNISRIYGKWYNLAIGSTCPWLKKIMDRMTVSTLVLGEGATEAEISMTSTRWRKGVCEETSGAYEKTDTDGKFLYHKSKWNITMESYVVHTNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLRETLLQDFRVVAQGVGIPEDSIFTMADRGECVPGEQEPEPILIPRVRRAVLPQEEEGSGGGQLVTEVTKKEDSCQLGYSAGPCMGMTSRYFYNGTSMACETFQYGGCMGNGNNFVTEKECLQTCRTVAACNLPIVRGPCRAFIQLWAFDAVKGKCVLFPYGGCQGNGNKFYSEKECREYCGVPGDGDEELLRFSN

Target ORF information:

RefSeq Version	NM_001633.3
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001633.3

ORF Insert Sequence:

ATGAGGAGCC TCGGGGCCCT GCTCTTGCTG CTGAGCGCCT GCCTGGCGGT GAGCGCTGGC 
CCTGTGCCAA CGCCGCCCGA CAACATCCAA GTGCAGGAAA ACTTCAATAT CTCTCGGATC 
TATGGGAAGT GGTACAACCT GGCCATCGGT TCCACCTGCC CCTGGCTGAA GAAGATCATG 
GACAGGATGA CAGTGAGCAC GCTGGTGCTG GGAGAGGGCG CTACAGAGGC GGAGATCAGC 
ATGACCAGCA CTCGTTGGCG GAAAGGTGTC TGTGAGGAGA CGTCTGGAGC TTATGAGAAA 
ACAGATACTG ATGGGAAGTT TCTCTATCAC AAATCCAAAT GGAACATAAC CATGGAGTCC 
TATGTGGTCC ACACCAACTA TGATGAGTAT GCCATTTTCC TGACCAAGAA ATTCAGCCGC 
CATCATGGAC CCACCATTAC TGCCAAGCTC TACGGGCGGG CGCCGCAGCT GAGGGAAACT 
CTCCTGCAGG ACTTCAGAGT GGTTGCCCAG GGTGTGGGCA TCCCTGAGGA CTCCATCTTC 
ACCATGGCTG ACCGAGGTGA ATGTGTCCCT GGGGAGCAGG AACCAGAGCC CATCTTAATC 
CCGAGAGTCC GGAGGGCTGT GCTACCCCAA GAAGAGGAAG GATCAGGGGG TGGGCAACTG 
GTAACTGAAG TCACCAAGAA AGAAGATTCC TGCCAGCTGG GCTACTCGGC CGGTCCCTGC 
ATGGGAATGA CCAGCAGGTA TTTCTATAAT GGTACATCCA TGGCCTGTGA GACTTTCCAG 
TACGGCGGCT GCATGGGCAA CGGTAACAAC TTCGTCACAG AAAAGGAGTG TCTGCAGACC 
TGCCGAACTG TGGCGGCCTG CAATCTCCCC ATAGTCCGGG GCCCCTGCCG AGCCTTCATC 
CAGCTCTGGG CATTTGATGC TGTCAAGGGG AAGTGCGTCC TCTTCCCCTA CGGGGGCTGC 
CAGGGCAACG GGAACAAGTT CTACTCAGAG AAGGAGTGCA GAGAGTACTG CGGTGTCCCT 
GGTGATGGTG ATGAGGAGCT GCTGCGCTTC TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBP ( NM_001633.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001624.1 Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.

Price & Availability↑

CloneID	OHu03642
Clone ID Related Accession (Same CDS sequence)	NM_001633.3 , NM_001633.4
Accession Version	NM_001633.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	protein AMBP preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC041593.1. On Nov 23, 2018 this sequence version replaced NM_001633.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041593.1, SRR5189652.12257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265132.8/ ENSP00000265132.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGAGCC TCGGGGCCCT GCTCTTGCTG CTGAGCGCCT GCCTGGCGGT GAGCGCTGGC 
CCTGTGCCAA CGCCGCCCGA CAACATCCAA GTGCAGGAAA ACTTCAATAT CTCTCGGATC 
TATGGGAAGT GGTACAACCT GGCCATCGGT TCCACCTGCC CCTGGCTGAA GAAGATCATG 
GACAGGATGA CAGTGAGCAC GCTGGTGCTG GGAGAGGGCG CTACAGAGGC GGAGATCAGC 
ATGACCAGCA CTCGTTGGCG GAAAGGTGTC TGTGAGGAGA CGTCTGGAGC TTATGAGAAA 
ACAGATACTG ATGGGAAGTT TCTCTATCAC AAATCCAAAT GGAACATAAC CATGGAGTCC 
TATGTGGTCC ACACCAACTA TGATGAGTAT GCCATTTTCC TGACCAAGAA ATTCAGCCGC 
CATCATGGAC CCACCATTAC TGCCAAGCTC TACGGGCGGG CGCCGCAGCT GAGGGAAACT 
CTCCTGCAGG ACTTCAGAGT GGTTGCCCAG GGTGTGGGCA TCCCTGAGGA CTCCATCTTC 
ACCATGGCTG ACCGAGGTGA ATGTGTCCCT GGGGAGCAGG AACCAGAGCC CATCTTAATC 
CCGAGAGTCC GGAGGGCTGT GCTACCCCAA GAAGAGGAAG GATCAGGGGG TGGGCAACTG 
GTAACTGAAG TCACCAAGAA AGAAGATTCC TGCCAGCTGG GCTACTCGGC CGGTCCCTGC 
ATGGGAATGA CCAGCAGGTA TTTCTATAAT GGTACATCCA TGGCCTGTGA GACTTTCCAG 
TACGGCGGCT GCATGGGCAA CGGTAACAAC TTCGTCACAG AAAAGGAGTG TCTGCAGACC 
TGCCGAACTG TGGCGGCCTG CAATCTCCCC ATAGTCCGGG GCCCCTGCCG AGCCTTCATC 
CAGCTCTGGG CATTTGATGC TGTCAAGGGG AAGTGCGTCC TCTTCCCCTA CGGGGGCTGC 
CAGGGCAACG GGAACAAGTT CTACTCAGAG AAGGAGTGCA GAGAGTACTG CGGTGTCCCT 
GGTGATGGTG ATGAGGAGCT GCTGCGCTTC TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001624.1
CDS	92..1150
Translation	MRSLGALLLLLSACLAVSAGPVPTPPDNIQVQENFNISRIYGKWYNLAIGSTCPWLKKIMDRMTVSTLVLGEGATEAEISMTSTRWRKGVCEETSGAYEKTDTDGKFLYHKSKWNITMESYVVHTNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLRETLLQDFRVVAQGVGIPEDSIFTMADRGECVPGEQEPEPILIPRVRRAVLPQEEEGSGGGQLVTEVTKKEDSCQLGYSAGPCMGMTSRYFYNGTSMACETFQYGGCMGNGNNFVTEKECLQTCRTVAACNLPIVRGPCRAFIQLWAFDAVKGKCVLFPYGGCQGNGNKFYSEKECREYCGVPGDGDEELLRFSN

Target ORF information:

RefSeq Version	NM_001633.4
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-1-microglobulin/bikunin precursor (AMBP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001633.4

ORF Insert Sequence:

ATGAGGAGCC TCGGGGCCCT GCTCTTGCTG CTGAGCGCCT GCCTGGCGGT GAGCGCTGGC 
CCTGTGCCAA CGCCGCCCGA CAACATCCAA GTGCAGGAAA ACTTCAATAT CTCTCGGATC 
TATGGGAAGT GGTACAACCT GGCCATCGGT TCCACCTGCC CCTGGCTGAA GAAGATCATG 
GACAGGATGA CAGTGAGCAC GCTGGTGCTG GGAGAGGGCG CTACAGAGGC GGAGATCAGC 
ATGACCAGCA CTCGTTGGCG GAAAGGTGTC TGTGAGGAGA CGTCTGGAGC TTATGAGAAA 
ACAGATACTG ATGGGAAGTT TCTCTATCAC AAATCCAAAT GGAACATAAC CATGGAGTCC 
TATGTGGTCC ACACCAACTA TGATGAGTAT GCCATTTTCC TGACCAAGAA ATTCAGCCGC 
CATCATGGAC CCACCATTAC TGCCAAGCTC TACGGGCGGG CGCCGCAGCT GAGGGAAACT 
CTCCTGCAGG ACTTCAGAGT GGTTGCCCAG GGTGTGGGCA TCCCTGAGGA CTCCATCTTC 
ACCATGGCTG ACCGAGGTGA ATGTGTCCCT GGGGAGCAGG AACCAGAGCC CATCTTAATC 
CCGAGAGTCC GGAGGGCTGT GCTACCCCAA GAAGAGGAAG GATCAGGGGG TGGGCAACTG 
GTAACTGAAG TCACCAAGAA AGAAGATTCC TGCCAGCTGG GCTACTCGGC CGGTCCCTGC 
ATGGGAATGA CCAGCAGGTA TTTCTATAAT GGTACATCCA TGGCCTGTGA GACTTTCCAG 
TACGGCGGCT GCATGGGCAA CGGTAACAAC TTCGTCACAG AAAAGGAGTG TCTGCAGACC 
TGCCGAACTG TGGCGGCCTG CAATCTCCCC ATAGTCCGGG GCCCCTGCCG AGCCTTCATC 
CAGCTCTGGG CATTTGATGC TGTCAAGGGG AAGTGCGTCC TCTTCCCCTA CGGGGGCTGC 
CAGGGCAACG GGAACAAGTT CTACTCAGAG AAGGAGTGCA GAGAGTACTG CGGTGTCCCT 
GGTGATGGTG ATGAGGAGCT GCTGCGCTTC TCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

The crystal structure of bikunin from the inter-alpha-inhibitor complex: a serine protease inhibitor with two Kunitz domains.
Journal of molecular biology276(5)955-66(1998 Mar)
Xu Y,Carr PD,Guss JM,Ollis DL

The complete amino acid sequence of human complex-forming glycoprotein heterogeneous in charge (protein HC) from one individual.
Archives of biochemistry and biophysics228(2)544-54(1984 Feb)
L?pez Otin C,Grubb AO,M?ndez E

Complete amino acid sequence of human alpha 1-microglobulin.
Biochemical and biophysical research communications98(4)997-1001(1981 Feb)
Takagi T,Takagi K,Kawai T

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AMBP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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