POLR1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR1A
Entrez Gene ID	25885
Full Name	RNA polymerase I subunit A
Synonyms	A190,AFDCIN,RPA1,RPA194,RPO1-4,RPO14
General protein information	Preferred Names RNA polymerase I subunit A Names DNA-directed RNA polymerase I subunit RPA1 DNA-directed RNA polymerase I largest subunit DNA-directed RNA polymerase I subunit A DNA-directed RNA polymerase I subunit A1 RNA polymerase I 194 kDa subunit polymerase (RNA) I polypeptide A, 194kDa polymerase (RNA) I subunit A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p11.2
Summary	The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016].
Disorder MIM:	616404
Disorder Html:	Acrofacial dysostosis, Cincinnati type, 616462 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015425.4	NP_056240.2	DNA-directed RNA polymerase I subunit RPA1
NM_015425.6	NP_056240.2	DNA-directed RNA polymerase I subunit RPA1
NM_015425.5	NP_056240.2	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulated long non-coding RNA LOC90784 promotes cell proliferation and invasion and is associated with poor clinical features in HCC.
Xu JH, Chang WH, Fu HW, Shu WQ, Yuan T, Chen P
Biochemical and biophysical research communications490(3)920-926(2017 08)

Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A.
Kara B, K?ro?lu ?, Peltonen K, Steinberg RC, Mara? Gen? H, H?ltt?-Vuori M, G?ven A, Kanerva K, Kotil T, Solako?lu S, Zhou Y, Olkkonen VM, Ikonen E, Laiho M, Tolun A
European journal of human genetics : EJHG25(3)315-323(2017 02)

The identification of a novel role for BRCA1 in regulating RNA polymerase I transcription.
Johnston R, D'Costa Z, Ray S, Gorski J, Harkin DP, Mullan P, Panov KI
Oncotarget7(42)68097-68110(2016 Oct)

The Warsaw breakage syndrome-related protein DDX11 is required for ribosomal RNA synthesis and embryonic development.
Sun X, Chen H, Deng Z, Hu B, Luo H, Zeng X, Han L, Cai G, Ma L
Human molecular genetics24(17)4901-15(2015 Sep)

Acrofacial Dysostosis, Cincinnati Type, a Mandibulofacial Dysostosis Syndrome with Limb Anomalies, Is Caused by POLR1A Dysfunction.
Weaver KN, Watt KE, Hufnagel RB, Navajas Acedo J, Linscott LL, Sund KL, Bender PL, K?nig R, Lourenco CM, Hehr U, Hopkin RJ, Lohmann DR, Trainor PA, Wieczorek D, Saal HM
American journal of human genetics96(5)765-74(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Homozygous c.2801C>T (p.(Ser934Leu)) in POLR1A was identified in two brothers with unusual neurological disease that manifested with ataxia, psychomotor retardation, cerebellar and cerebral atrophy, and leukodystrophy.
Title: Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A.

Consistent with this observation, we discovered that polr1a mutant zebrafish exhibited cranioskeletal anomalies mimicking the human phenotype. polr1a loss of function led to perturbed ribosome biogenesis and p53-dependent cell death
Title: Acrofacial Dysostosis, Cincinnati Type, a Mandibulofacial Dysostosis Syndrome with Limb Anomalies, Is Caused by POLR1A Dysfunction.

SIRT7 knockdown leads to reduced levels of RNA Pol I protein, but not messenger RNA
Title: Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription.

findings demonstrate that Bloom's syndrome helicase (BLM)interacts with RPA194; studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription
Title: BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.

Interference of POLR1A inhibited the synthesis of rRNA and hindered cell cycle progression in cells with inactivated p53, as a consequence of downregulation of the transcription factor E2F-1.
Title: Selective inhibition of rRNA transcription downregulates E2F-1: a new p53-independent mechanism linking cell growth to cell proliferation.

The following POLR1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06985	NM_015425.6 Latest version!	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu06985	NM_015425.4	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu06985	NM_015425.5	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR1A ( NM_015425.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056240.2 Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

Price & Availability↑

CloneID	OHu06985
Clone ID Related Accession (Same CDS sequence)	NM_015425.4 , NM_015425.6 , NM_015425.5
Accession Version	NM_015425.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Homo sapiens(Human)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363163.1, BC126303.1, AC012511.7 and BQ653001.1. This sequence is a reference standard in the RefSeqGene project. On May 18, 2016 this sequence version replaced NM_015425.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.216742.1, SRR1803616.231740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056240.2
CDS	380..5542
Translation	MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_015425.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015425.4

ORF Insert Sequence:

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR1A ( NM_015425.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_056240.2 Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

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CloneID	OHu06985
Clone ID Related Accession (Same CDS sequence)	NM_015425.4 , NM_015425.6 , NM_015425.5
Accession Version	NM_015425.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363163.1, AK302458.1, AK307490.1, AC012511.7 and BC073824.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015425.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.216742.1, SRR1803616.231740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263857.11/ ENSP00000263857.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056240.2
CDS	111..5273
Translation	MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_015425.6
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015425.6

ORF Insert Sequence:

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR1A ( NM_015425.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056240.2 Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

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CloneID	OHu06985
Clone ID Related Accession (Same CDS sequence)	NM_015425.4 , NM_015425.6 , NM_015425.5
Accession Version	NM_015425.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363163.1, AK302458.1, AK307490.1, AC012511.7 and BC073824.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 28, 2017 this sequence version replaced NR_026984.1, NM_015425.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.216742.1, SRR1803616.231740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056240.2
CDS	380..5542
Translation	MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_015425.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase I subunit A (POLR1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015425.5

ORF Insert Sequence:

ATGTTGATCT CCAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCTGAAG AGCTCAAGAA ATTAAGTGTT AAATCCATTA CGAACCCTCG ATACCTGGAC 
AGCCTGGGGA ACCCATCGGC AAACGGCCTG TACGATTTAG CTTTGGGCCC TGCAGATTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCAGCAACT GTTCTGGGCA CCTGGGCCAC 
ATTGAGCTCC CACTCACAGT GTATAACCCT CTCCTCTTCG ATAAGCTGTA CCTGCTGCTT 
CGGGGCTCTT GTTTAAACTG CCACATGCTG ACTTGTCCCC GGGCCGTGAT TCACCTCTTA 
CTCTGCCAGC TGAGGGTTCT GGAAGTCGGG GCCCTACAAG CAGTCTACGA GCTTGAGAGA 
ATTCTGAACA GGTTTCTGGA AGAAAATCCC GATCCCTCTG CCTCTGAAAT TCGGGAGGAA 
TTAGAACAAT ACACAACTGA AATTGTGCAG AACAACCTCC TGGGGTCCCA GGGCGCACAT 
GTAAAGAACG TGTGTGAGAG CAAGAGCAAG CTCATTGCTC TCTTCTGGAA GGCACATATG 
AATGCTAAGC GCTGTCCCCA CTGCAAGACC GGGCGATCCG TTGTCCGAAA GGAACACAAC 
AGCAAGTTGA CTATCACGTT TCCAGCCATG GTGCACAGGA CAGCTGGCCA GAAGGACTCT 
GAGCCCCTGG GAATTGAGGA AGCTCAGATA GGAAAACGAG GATACTTAAC ACCCACCAGT 
GCCCGCGAAC ACCTTTCTGC CCTGTGGAAG AATGAAGGAT TCTTTCTGAA CTACCTTTTT 
TCGGGAATGG ATGATGATGG TATGGAATCC AGATTCAATC CCAGTGTGTT CTTTCTAGAT 
TTCTTGGTGG TGCCGCCCTC AAGGTATCGC CCAGTCAGTC GCCTAGGAGA CCAGATGTTT 
ACTAATGGCC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ATGTAGTTCT GATTCGAAAA 
CTTCTGGCAT TGATGGCCCA AGAACAGAAG TTGCCAGAGG AAGTGGCCAC ACCCACTACA 
GATGAGGAAA AAGACTCTTT GATTGCTATT GACCGATCCT TTTTGAGTAC ACTTCCAGGC 
CAGTCCCTCA TAGACAAACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTCAATATT 
GTGTTTGATA GCGAGATGGA CAAACTAATG ATGGACAAGT ACCCAGGCAT TAGGCAGATC 
CTGGAGAAGA AAGAAGGCCT GTTCCGAAAA CACATGATGG GAAAGCGAGT GGACTACGCT 
GCGCGCTCAG TCATCTGCCC AGACATGTAC ATCAACACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAACTGAC CTACCCACAG CCAGTTACCC CATGGAATGT TCAGGAACTT 
AGGCAAGCGG TCATCAACGG CCCTAATGTG CACCCAGGAG CCTCCATGGT CATCAATGAG 
GACGGCAGCC GCACAGCCCT GAGCGCTGTG GACATGACCC AGCGAGAGGC CGTGGCCAAG 
CAGCTTCTGA CCCCAGCCAC GGGGGCACCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ATGGGGACAT TCTGCTACTG AACCGACAGC CCACACTGCA CAGACCCTCC 
ATCCAGGCCC ACCGTGCCCG CATCCTGCCT GAAGAGAAAG TGCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTATAATGC CGACTTTGAT GGAGACGAGA TGAATGCCCA TTTCCCCCAG 
AGTGAGCTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CTGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAACCATT GGCGGGACTG ATCCAGGATC ACATGGTTTC AGGGGCAAGC 
ATGACTACTC GGGGTTGCTT TTTCACCCGG GAGCACTATA TGGAGCTGGT GTACCGAGGA 
CTCACGGACA AAGTGGGGCG CGTGAAGCTC CTTTCTCCTT CCATCCTGAA GCCCTTTCCG 
CTGTGGACAG GAAAACAGGT TGTGTCAACG CTGCTCATAA ATATAATCCC AGAGGACCAC 
ATCCCACTGA ACTTATCTGG AAAGGCGAAA ATCACTGGGA AAGCCTGGGT GAAGGAAACT 
CCTCGATCCG TTCCTGGCTT TAACCCTGAC TCGATGTGCG AGTCCCAGGT GATCATCAGG 
GAAGGGGAGC TGCTCTGCGG AGTGCTGGAC AAGGCGCACT ATGGGAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTATGA GATCTATGGA GGCGAGACCA GCGGCAAGGT TCTAACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGCGTGGAA 
GACATTTTGG TGAAGCCAAA GGCAGATGTC AAGAGGCAAC GTATCATTGA AGAATCCACC 
CACTGCGGGC CCCAGGCTGT CAGGGCTGCA TTAAACCTGC CAGAAGCCGC ATCATATGAT 
GAGGTCCGAG GAAAATGGCA GGATGCCCAT CTGGGCAAGG ACCAGAGGGA TTTTAACATG 
ATTGATCTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAGATTAA CAAGGCATGC 
ATGCCTTTTG GCCTACACAG ACAGTTCCCA GAGAACAGCC TGCAGATGAT GGTGCAGTCG 
GGAGCCAAAG GTTCAACTGT GAACACGATG CAGATCTCGT GCCTGCTGGG CCAGATTGAA 
CTGGAAGGTC GGAGACCCCC GCTGATGGCG TCTGGCAAGT CACTGCCCTG CTTTGAGCCT 
TATGAGTTCA CCCCCAGGGC TGGTGGCTTT GTCACTGGCA GGTTCCTCAC CGGCATCAAA 
CCTCCTGAGT TCTTCTTCCA CTGCATGGCA GGACGAGAGG GCCTGGTGGA CACTGCTGTG 
AAAACCAGCC GCTCAGGCTA TCTCCAAAGG TGCATCATCA AGCACCTAGA GGGGCTGGTC 
GTGCAGTATG ATCTCACGGT CCGTGACAGT GACGGCAGTG TGGTGCAGTT CCTGTATGGG 
GAGGATGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CCAAGCAGTT CCCCTTCCTG 
GCCAGCAACT ACGAGGTGAT AATGAAATCA CAGCATCTCC ATGAAGTTTT ATCCAGAGCA 
GATCCCAAAA AAGCTCTCCA CCACTTCAGA GCTATCAAAA AATGGCAAAG CAAGCACCCC 
AACACCCTGC TGAGAAGAGG CGCCTTCTTG AGTTATTCCC AGAAAATTCA GGAAGCTGTG 
AAAGCCCTGA AACTTGAGAG TGAAAACCGC AATGGCCGCA GCCCTGGGAC TCAGGAGATG 
CTGAGGATGT GGTATGAGTT GGATGAGGAA AGCCGAAGGA AATACCAGAA GAAGGCGGCC 
GCTTGTCCTG ACCCCAGTCT GTCTGTCTGG CGTCCTGACA TCTACTTTGC ATCAGTGTCA 
GAAACATTTG AAACAAAGGT TGATGACTAC AGTCAAGAGT GGGCAGCTCA AACAGAGAAG 
AGTTATGAGA AATCAGAGCT TTCTCTCGAC AGGTTGAGGA CCTTGCTGCA GCTGAAGTGG 
CAGCGCTCAC TGTGTGAGCC GGGCGAGGCT GTGGGCCTGC TGGCTGCCCA GAGCATCGGA 
GAGCCCTCCA CCCAGATGAC CCTCAACACC TTCCACTTTG CAGGCAGAGG CGAGATGAAC 
GTCACCCTGG GCATTCCAAG GTTGCGGGAG ATTCTCATGG TGGCCAGCGC CAACATCAAG 
ACACCCATGA TGAGCGTGCC CGTGCTCAAC ACCAAGAAAG CCCTGAAGAG AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG GGTGTGCTTG GGGGAGGTGT TGCAGAAAAT TGACGTCCAG 
GAGTCCTTCT GTATGGAAGA AAAACAGAAC AAATTCCAGG TGTACCAGCT GCGGTTTCAG 
TTCCTGCCAC ATGCATATTA CCAGCAGGAG AAGTGCCTGA GACCCGAGGA CATCCTGCGC 
TTCATGGAAA CAAGATTCTT TAAACTTCTG ATGGAATCCA TCAAAAAGAA GAATAATAAA 
GCATCAGCTT TCAGGAACGT AAACACTCGA AGAGCTACAC AGCGGGATCT GGACAACGCT 
GGGGAGTTGG GGAGGAGTCG GGGAGAGCAG GAGGGTGATG AGGAAGAGGA GGGGCACATT 
GTGGATGCTG AAGCTGAGGA GGGGGACGCC GATGCCTCTG ATGCCAAACG CAAGGAGAAG 
CAGGAGGAGG AGGTTGATTA TGAGAGTGAG GAAGAGGAGG AGAGGGAGGG CGAGGAGAAC 
GACGATGAAG ACATGCAGGA GGAACGAAAT CCCCACAGGG AAGGTGCTCG AAAGACCCAA 
GAGCAAGATG AAGAGGTGGG CTTAGGCACT GAGGAGGACC CGTCCCTTCC CGCCCTCCTG 
ACGCAGCCCC GGAAACCCAC CCACAGCCAG GAGCCCCAGG GGCCCGAGGC CATGGAGCGC 
CGGGTCCAGG CTGTGCGTGA GATCCACCCG TTCATAGATG ACTACCAGTA CGACACCGAG 
GAGAGCCTGT GGTGCCAGGT GACAGTGAAG CTCCCTCTGA TGAAGATCAA CTTTGACATG 
AGCTCCCTGG TAGTATCTTT GGCCCATGGT GCCGTCATCT ATGCGACCAA GGGCATCACT 
CGGTGCCTCC TGAATGAAAC AACCAACAAT AAGAACGAGA AGGAGCTTGT GCTAAACACA 
GAAGGAATCA ACCTCCCAGA GCTATTCAAG TATGCAGAGG TCCTGGATCT GCGCCGCCTC 
TACTCCAACG ACATCCACGC CATAGCCAAC ACGTATGGCA TTGAGGCCGC GCTGCGGGTG 
ATCGAGAAGG AGATCAAGGA TGTGTTTGCC GTGTATGGCA TCGCGGTCGA CCCTCGCCAT 
CTCTCCCTGG TTGCTGATTA TATGTGCTTC GAGGGTGTTT ACAAGCCACT GAATCGCTTT 
GGGATCCGGT CAAACTCTTC CCCGCTACAG CAGATGACAT TTGAAACCAG CTTCCAGTTT 
CTGAAGCAAG CCACCATGCT GGGATCCCAC GATGAGCTGA GGTCTCCTTC TGCCTGCCTT 
GTGGTCGGGA AGGTCGTCAG GGGCGGGACA GGCCTGTTCG AGCTCAAGCA GCCTCTGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulated long non-coding RNA LOC90784 promotes cell proliferation and invasion and is associated with poor clinical features in HCC.
Biochemical and biophysical research communications490(3)920-926(2017 08)
Xu JH,Chang WH,Fu HW,Shu WQ,Yuan T,Chen P

Severe neurodegenerative disease in brothers with homozygous mutation in POLR1A.
European journal of human genetics : EJHG25(3)315-323(2017 02)
Kara B,K?ro?lu ?,Peltonen K,Steinberg RC,Mara? Gen? H,H?ltt?-Vuori M,G?ven A,Kanerva K,Kotil T,Solako?lu S,Zhou Y,Olkkonen VM,Ikonen E,Laiho M,Tolun A

The identification of a novel role for BRCA1 in regulating RNA polymerase I transcription.
Oncotarget7(42)68097-68110(2016 Oct)
Johnston R,D'Costa Z,Ray S,Gorski J,Harkin DP,Mullan P,Panov KI

The Warsaw breakage syndrome-related protein DDX11 is required for ribosomal RNA synthesis and embryonic development.
Human molecular genetics24(17)4901-15(2015 Sep)
Sun X,Chen H,Deng Z,Hu B,Luo H,Zeng X,Han L,Cai G,Ma L

Acrofacial Dysostosis, Cincinnati Type, a Mandibulofacial Dysostosis Syndrome with Limb Anomalies, Is Caused by POLR1A Dysfunction.
American journal of human genetics96(5)765-74(2015 May)
Weaver KN,Watt KE,Hufnagel RB,Navajas Acedo J,Linscott LL,Sund KL,Bender PL,K?nig R,Lourenco CM,Hehr U,Hopkin RJ,Lohmann DR,Trainor PA,Wieczorek D,Saal HM

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POLR1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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