ORF » Species Summary » Homo sapiens » SPEF1 cDNA ORF clone

SPEF1 cDNA ORF clone, Homo sapiens(human)

The following SPEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06982 NM_015417.5
Latest version!		 Homo sapiens sperm flagellar 1 (SPEF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu52689 XM_005260683.4
Latest version!		 Homo sapiens sperm flagellar 1 (SPEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu06982 NM_015417.4 Homo sapiens sperm flagellar 1 (SPEF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPEF1 ( NM_015417.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056232.2 Homo sapiens sperm flagellar 1 (SPEF1), mRNA.
    Price & Availability↑
    CloneID OHu06982
    Clone ID Related Accession (Same CDS sequence) NM_015417.5 , NM_015417.4
    Accession Version NM_015417.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Homo sapiens(human)
    Product sperm flagellar protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB030684.1, DB033051.1, AL040853.1, AL080154.1 and AI925267.1. On Nov 22, 2018 this sequence version replaced NM_015417.4. ##Evidence-Data-START## Transcript exon combination :: EF560745.1, BI520334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379756.3/ ENSP00000369080.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGC 
    TGGGACCGGC CGCCGGCGCC TCGGCCTCCA GCGTATAACC GGGCGTTGCA GGGCGACCCC 
    AGCTTCGTCC TCCAGATCGC TGAAAAGGAG CAGGAGCTGT TGGCCTCTCA AGAGACCGTG 
    CAGGTCCTGC AGATGAAGGT AAGGCGCCTG GAGCACCTGC TCCAGCTCAA GAATGTGCGG 
    ATCGAAGACC TCTCCCGGCG GCTCCAGCAG GCGGAGCGTA AGCAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056232.2
    CDS162..872
    Translation

    Target ORF information:

    RefSeq Version NM_015417.5
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 1 (SPEF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_015417.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGC 
    TGGGACCGGC CGCCGGCGCC TCGGCCTCCA GCGTATAACC GGGCGTTGCA GGGCGACCCC 
    AGCTTCGTCC TCCAGATCGC TGAAAAGGAG CAGGAGCTGT TGGCCTCTCA AGAGACCGTG 
    CAGGTCCTGC AGATGAAGGT AAGGCGCCTG GAGCACCTGC TCCAGCTCAA GAATGTGCGG 
    ATCGAAGACC TCTCCCGGCG GCTCCAGCAG GCGGAGCGTA AGCAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPEF1 ( NM_015417.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056232.2 Homo sapiens sperm flagellar 1 (SPEF1), mRNA.
    Price & Availability↑
    CloneID OHu06982
    Clone ID Related Accession (Same CDS sequence) NM_015417.5 , NM_015417.4
    Accession Version NM_015417.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product sperm flagellar protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB030684.1, DB033051.1, AL040853.1, AL080154.1 and AI925267.1. On Sep 16, 2006 this sequence version replaced NM_015417.3. ##Evidence-Data-START## Transcript exon combination :: EF560745.1, BI520334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGC 
    TGGGACCGGC CGCCGGCGCC TCGGCCTCCA GCGTATAACC GGGCGTTGCA GGGCGACCCC 
    AGCTTCGTCC TCCAGATCGC TGAAAAGGAG CAGGAGCTGT TGGCCTCTCA AGAGACCGTG 
    CAGGTCCTGC AGATGAAGGT AAGGCGCCTG GAGCACCTGC TCCAGCTCAA GAATGTGCGG 
    ATCGAAGACC TCTCCCGGCG GCTCCAGCAG GCGGAGCGTA AGCAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056232.2
    CDS169..879
    Translation

    Target ORF information:

    RefSeq Version NM_015417.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 1 (SPEF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_015417.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGC 
    TGGGACCGGC CGCCGGCGCC TCGGCCTCCA GCGTATAACC GGGCGTTGCA GGGCGACCCC 
    AGCTTCGTCC TCCAGATCGC TGAAAAGGAG CAGGAGCTGT TGGCCTCTCA AGAGACCGTG 
    CAGGTCCTGC AGATGAAGGT AAGGCGCCTG GAGCACCTGC TCCAGCTCAA GAATGTGCGG 
    ATCGAAGACC TCTCCCGGCG GCTCCAGCAG GCGGAGCGTA AGCAGCGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPEF1 ( XM_005260683.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260740.1 Homo sapiens sperm flagellar 1 (SPEF1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu52689
    Clone ID Related Accession (Same CDS sequence) XM_005260683.4
    Accession Version XM_005260683.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product sperm flagellar protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260683.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGG 
    GCGACCCCAG CTTCGTCCTC CAGATCGCTG AAAAGGAGCA GGAGCTGTTG GCCTCTCAAG 
    AGACCGTGCA GGTCCTGCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260740.1
    CDS180..743
    Translation

    Target ORF information:

    RefSeq Version XM_005260683.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 1 (SPEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005260683.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCGAGCA GCGTGGACGA GGAGGCGCTG CACCAGCTGT ACCTGTGGGT AGACAACATC 
    CCTCTGTCCC GGCCCAAGCG AAACCTCTCC CGGGACTTTA GCGATGGAGT CCTTGTTGCA 
    GAGGTCATCA AGTTTTACTT CCCCAAGATG GTGGAGATGC ACAATTATGT CCCCGCCAAC 
    TCTCTCCAGC AGAAGCTCAG CAACTGGGGT CATCTGAACA GGAAGGTACT GAAGAGGCTG 
    AACTTTTCAG TACCGGATGA CGTGATGCGC AAGATCGCGC AGTGCGCCCC AGGCGTGGTG 
    GAGCTGGTGC TCATCCCGCT GAGGCAGCGC CTGGAGGAGA GGCAGAGGCG CAGGAAGCAG 
    GGCGCCGGCT CCTTACAGGA GCTGGCTCCC CAGGATGGCA GTGGCTACAT GGATGTGGGT 
    GTATCCCAGA AGGCCCGAGG TGAAGGTGTC CCGGACCCCC AGGGAGGGGG TCAGCTCAGG 
    GCGACCCCAG CTTCGTCCTC CAGATCGCTG AAAAGGAGCA GGAGCTGTTG GCCTCTCAAG 
    AGACCGTGCA GGTCCTGCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.
    The Journal of cell biology206(3)367-76(2014 Aug)
    Werner ME,Mitchell JW,Putzbach W,Bacon E,Kim SK,Mitchell BJ


    CLAMP, a novel microtubule-associated protein with EB-type calponin homology.
    Cell motility and the cytoskeleton62(3)141-56(2005 Nov)
    Dougherty GW,Adler HJ,Rzadzinska A,Gimona M,Tomita Y,Lattig MC,Merritt RC Jr,Kachar B


    Spef1, a conserved novel testis protein found in mouse sperm flagella.
    Gene353(2)189-99(2005 Jul)
    Chan SW,Fowler KJ,Choo KH,Kalitsis P


    The DNA sequence and comparative analysis of human chromosome 20.
    Nature414(6866)865-71(2001 Dec 20-27)
    Deloukas P,Matthews LH,Ashurst J,Burton J,Gilbert JG,Jones M,Stavrides G,Almeida JP,Babbage AK,Bagguley CL,Bailey J,Barlow KF,Bates KN,Beard LM,Beare DM,Beasley OP,Bird CP,Blakey SE,Bridgeman AM,Brown AJ,Buck D,Burrill W,Butler AP,Carder C,Carter NP,Chapman JC,Clamp M,Clark G,Clark LN,Clark SY,Clee CM,Clegg S,Cobley VE,Collier RE,Connor R,Corby NR,Coulson A,Coville GJ,Deadman R,Dhami P,Dunn M,Ellington AG,Frankland JA,Fraser A,French L,Garner P,Grafham DV,Griffiths C,Griffiths MN,Gwilliam R,Hall RE,Hammond S,Harley JL,Heath PD,Ho S,Holden JL,Howden PJ,Huckle E,Hunt AR,Hunt SE,Jekosch K,Johnson CM,Johnson D,Kay MP,Kimberley AM,King A,Knights A,Laird GK,Lawlor S,Lehvaslaiho MH,Leversha M,Lloyd C,Lloyd DM,Lovell JD,Marsh VL,Martin SL,McConnachie LJ,McLay K,McMurray AA,Milne S,Mistry D,Moore MJ,Mullikin JC,Nickerson T,Oliver K,Parker A,Patel R,Pearce TA,Peck AI,Phillimore BJ,Prathalingam SR,Plumb RW,Ramsay H,Rice CM,Ross MT,Scott CE,Sehra HK,Shownkeen R,Sims S,Skuce CD,Smith ML,Soderlund C,Steward CA,Sulston JE,Swann M,Sycamore N,Taylor R,Tee L,Thomas DW,Thorpe A,Tracey A,Tromans AC,Vaudin M,Wall M,Wallis JM,Whitehead SL,Whittaker P,Willey DL,Williams L,Williams SA,Wilming L,Wray PW,Hubbard T,Durbin RM,Bentley DR,Beck S,Rogers J


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