USP49 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP49
Entrez Gene ID	25862
Full Name	ubiquitin specific peptidase 49
General protein information	Preferred Names ubiquitin specific peptidase 49 Names ubiquitin carboxyl-terminal hydrolase 49 deubiquitinating enzyme 49 ubiquitin specific protease 49 ubiquitin thioesterase 49 ubiquitin thiolesterase 49 ubiquitin-specific-processing protease 49
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_018561.4	NP_061031.2	ubiquitin carboxyl-terminal hydrolase 49 isoform b
NM_001286554.1	NP_001273483.1	ubiquitin carboxyl-terminal hydrolase 49 isoform a
NM_018561.5	NP_061031.2	ubiquitin carboxyl-terminal hydrolase 49 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP49	NP_001273483.1
Bos taurus (cattle)	USP49	XP_005223589.1
Mus musculus (house mouse)	Usp49	NP_940813.1
Rattus norvegicus (Norway rat)	Usp49	NP_001129942.1
Pan troglodytes (chimpanzee)	USP49	XP_518467.3
Canis lupus familiaris (dog)	USP49	XP_532134.3
Xenopus tropicalis (tropical clawed frog)	usp49	XP_004910733.1
	USP49	XP_001085366.1
Gallus gallus (chicken)	USP49	XP_001231682.1
Danio rerio (zebrafish)	usp49	NP_001038361.1

Gene Ontology
Bibliography

Related articles in PubMed

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.
Luo K, Li Y, Yin Y, Li L, Wu C, Chen Y, Nowsheen S, Hu Q, Zhang L, Lou Z, Yuan J
The EMBO journal36(10)1434-1446(2017 05)

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, Berger B, Gingras AC, Lindquist S
Cell158(2)434-448(2014 Jul)

USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.
Zhang Z, Jones A, Joo HY, Zhou D, Cao Y, Chen S, Erdjument-Bromage H, Renfrow M, He H, Tempst P, Townes TM, Giles KE, Ma L, Wang H
Genes & development27(14)1581-95(2013 Jul)

GWAS of blood cell traits identifies novel associated loci and epistatic interactions in Caucasian and African-American children.
Li J, Glessner JT, Zhang H, Hou C, Wei Z, Bradfield JP, Mentch FD, Guo Y, Kim C, Xia Q, Chiavacci RM, Thomas KA, Qiu H, Grant SF, Furth SL, Hakonarson H, Sleiman PM
Human molecular genetics22(7)1457-64(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP's capability to dephosphorylate AKT.
Title: USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.

identifies USP49 as a histone H2B-specific deubiquitinase and uncovers a critical role for H2B deubiquitination in cotranscriptional pre-mRNA processing events.
Title: USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.

The following USP49 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP49 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16930	NM_018561.5 Latest version!	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu20532	NM_001286554.1 Latest version!	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu16930	NM_018561.4	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP49 ( NM_018561.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061031.2 Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16930
Clone ID Related Accession (Same CDS sequence)	NM_018561.4 , NM_018561.5
Accession Version	NM_018561.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC014176.2 and AJ586139.1. On Nov 8, 2013 this sequence version replaced NM_018561.3. Transcript Variant: This variant (2) differs in the 5' UTR, and it includes alternate 3' exon structure and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BC014176.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTGC GTGCGCTTTA CTCTGTGGGG TGGGGGACAC GGAAAGGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061031.2
CDS	223..2145
Translation	MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTERG

Target ORF information:

RefSeq Version	NM_018561.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018561.4

ORF Insert Sequence:

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTGC GTGCGCTTTA CTCTGTGGGG TGGGGGACAC GGAAAGGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP49 ( NM_018561.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061031.2 Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16930
Clone ID Related Accession (Same CDS sequence)	NM_018561.4 , NM_018561.5
Accession Version	NM_018561.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL160163.24, BC014176.2 and AJ586139.1. On Jun 2, 2019 this sequence version replaced NM_018561.4. Transcript Variant: This variant (2) differs in the 5' UTR, and it includes alternate 3' exon structure and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BC014176.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTGC GTGCGCTTTA CTCTGTGGGG TGGGGGACAC GGAAAGGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061031.2
CDS	237..2159
Translation	MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTERG

Target ORF information:

RefSeq Version	NM_018561.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018561.5

ORF Insert Sequence:

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTGC GTGCGCTTTA CTCTGTGGGG TGGGGGACAC GGAAAGGGGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP49 ( NM_001286554.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273483.1 Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20532
Clone ID Related Accession (Same CDS sequence)	NM_001286554.1
Accession Version	NM_001286554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC014176.2, AJ586139.1, DN992189.1, AL365205.35 and BU631853.1. On Nov 8, 2013 this sequence version replaced XM_005248991.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: SRR1803613.272430.1, AJ586139.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTTT TTGGGTCCAC TGCAATGACT CAAAGCTGAA TGTATGCAGT 
GTCGAGGAAG TGTGCAAAAC CCAGGCCTAC ATCCTTTTTT ACACTCAAAG AACAGTGCAG 
GGCAATGCAA GAATCTCAGA AACCCATCTC CAAGCTCAGG TGCAGTCCAG CAACAATGAT 
GAAGGCAGAC CACAGACATT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273483.1
CDS	103..2169
Translation	MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSKLNVCSVEEVCKTQAYILFYTQRTVQGNARISETHLQAQVQSSNNDEGRPQTFS

Target ORF information:

RefSeq Version	NM_001286554.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 49 (USP49), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286554.1

ORF Insert Sequence:

ATGGATAGAT GCAAACATGT AGGGCGGTTA CGGCTCGCCC AGGACCACTC CATCCTGAAC 
CCTCAGAAGT GGTGCTGCTT AGAGTGTGCC ACCACCGAGT CCGTGTGGGC CTGCCTCAAG 
TGCTCCCACG TGGCCTGCGG CCGCTATATT GAGGACCACG CCCTGAAACA CTTTGAGGAG 
ACGGGACACC CGCTAGCCAT GGAAGTCCGG GATCTCTACG TGTTCTGTTA CCTGTGCAAG 
GACTACGTGC TCAATGATAA CCCAGAGGGG GACCTGAAGC TGCTAAGAAG CTCCCTCCTG 
GCGGTCCGGG GCCAGAAACA GGACACGCCG GTGAGACGTG GGCGGACGCT GCGGTCCATG 
GCTTCGGGTG AGGACGTGGT CCTGCCGCAG CGCGCTCCTC AGGGACAGCC GCAGATGCTC 
ACGGCTCTGT GGTACCGGCG TCAGCGCCTG CTGGCCAGGA CGCTGCGGCT GTGGTTCGAG 
AAGAGCTCCC GGGGCCAGGC GAAGCTGGAG CAGCGGCGGC AGGAGGAGGC CCTGGAGCGC 
AAGAAGGAGG AGGCGCGGAG GCGGCGGCGC GAGGTGAAAC GGCGGCTGCT GGAGGAGCTG 
GCCAGCACCC CTCCGCGCAA GAGTGCACGG CTGCTCCTGC ACACGCCCCG CGACGCGGGC 
CCGGCTGCCT CGCGCCCCGC CGCCCTCCCT ACCTCACGCA GAGTGCCCGC CGCCACACTC 
AAGCTGCGTC GCCAGCCGGC CATGGCCCCA GGCGTCACGG GCCTGCGCAA CCTGGGCAAC 
ACCTGCTACA TGAACTCCAT CCTCCAGGTG CTCAGCCACC TCCAGAAGTT CCGAGAATGT 
TTCCTCAACC TTGACCCTTC CAAAACGGAA CATCTGTTTC CCAAAGCCAC CAACGGGAAG 
ACTCAGCTTT CTGGCAAGCC AACCAACAGC TCGGCCACGG AGCTGTCCTT GAGAAATGAC 
AGGGCCGAGG CATGCGAGCG GGAGGGCTTC TGCTGGAACG GCAGGGCCTC CATTAGTCGG 
AGTCTGGAGC TCATCCAGAA CAAGGAGCCG AGTTCAAAGC ACATTTCCCT CTGCCGTGAA 
CTGCACACCC TCTTCCGAGT CATGTGGTCC GGGAAGTGGG CCCTAGTGTC GCCCTTCGCC 
ATGCTCCACT CAGTGTGGAG CCTGATCCCT GCCTTCCGCG GCTACGACCA ACAGGACGCG 
CAGGAATTTC TCTGCGAGCT GCTGCACAAG GTGCAGCAGG AACTCGAGTC TGAGGGCACC 
ACACGCCGGA TCCTCATCCC CTTCTCCCAG AGGAAGCTCA CCAAACAGGT CTTAAAGGTG 
GTGAATACCA TATTTCATGG GCAGCTGCTC AGTCAGGTCA CATGTATATC ATGCAATTAC 
AAATCCAATA CCATTGAGCC CTTTTGGGAC CTATCCCTGG AATTCCCTGA ACGCTATCAC 
TGCATAGAAA AGGGGTTTGT CCCTTTGAAT CAAACAGAGT GCTTGCTCAC TGAGATGCTG 
GCCAAATTCA CAGAGACAGA GGCCCTGGAA GGGAGAATCT ACGCTTGTGA CCAGTGTAAC 
AGCAAACGAC GAAAATCCAA TCCCAAACCC CTTGTTCTGA GTGAAGCTAG AAAGCAGTTA 
ATGATCTACA GACTACCTCA GGTTCTCCGG CTGCACCTTA AAAGATTCAG GTGGTCTGGC 
CGTAATCATC GAGAGAAGAT TGGGGTCCAT GTCGTCTTTG ACCAGGTATT AACCATGGAA 
CCTTACTGCT GCAGGGACAT GCTCTCCTCT CTTGACAAAG AGACCTTTGC CTATGATCTC 
TCCGCAGTGG TCATGCATCA CGGGAAAGGG TTTGGCTCAG GACACTACAC AGCCTATTGC 
TACAACACAG AGGGAGGTTT TTGGGTCCAC TGCAATGACT CAAAGCTGAA TGTATGCAGT 
GTCGAGGAAG TGTGCAAAAC CCAGGCCTAC ATCCTTTTTT ACACTCAAAG AACAGTGCAG 
GGCAATGCAA GAATCTCAGA AACCCATCTC CAAGCTCAGG TGCAGTCCAG CAACAATGAT 
GAAGGCAGAC CACAGACATT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.
The EMBO journal36(10)1434-1446(2017 05)
Luo K,Li Y,Yin Y,Li L,Wu C,Chen Y,Nowsheen S,Hu Q,Zhang L,Lou Z,Yuan J

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
Cell158(2)434-448(2014 Jul)
Taipale M,Tucker G,Peng J,Krykbaeva I,Lin ZY,Larsen B,Choi H,Berger B,Gingras AC,Lindquist S

USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.
Genes & development27(14)1581-95(2013 Jul)
Zhang Z,Jones A,Joo HY,Zhou D,Cao Y,Chen S,Erdjument-Bromage H,Renfrow M,He H,Tempst P,Townes TM,Giles KE,Ma L,Wang H

GWAS of blood cell traits identifies novel associated loci and epistatic interactions in Caucasian and African-American children.
Human molecular genetics22(7)1457-64(2013 Apr)
Li J,Glessner JT,Zhang H,Hou C,Wei Z,Bradfield JP,Mentch FD,Guo Y,Kim C,Xia Q,Chiavacci RM,Thomas KA,Qiu H,Grant SF,Furth SL,Hakonarson H,Sleiman PM

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USP49 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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