GALC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GALC
Entrez Gene ID	2581
Full Name	galactosylceramidase
General protein information	Preferred Names galactosylceramidase Names galactocerebrosidase GALCERase galactocerebroside beta-galactosidase galactosylceramide beta-galactosidase galactosylceraminidase testis tissue sperm-binding protein Li 88E testis tissue sperm-binding protein Li 89A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q31.3
Summary	This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	606890
Disorder Html:	Krabbe disease, 245200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000153.4	NP_000144.2	galactocerebrosidase isoform a precursor
XM_011536618.2	XP_011534920.1	galactocerebrosidase isoform X1
NM_001201401.1	NP_001188330.1	galactocerebrosidase isoform c precursor
NM_000153.3	NP_000144.2	galactocerebrosidase isoform a precursor
NM_001201402.1	NP_001188331.1	galactocerebrosidase isoform d

Pathways from BioSystems

Homologies

Homo sapiens (human)	GALC	NP_000144.2
Danio rerio (zebrafish)	galcb	NP_998276.1
Caenorhabditis elegans (roundworm)	CELE_C29E4.10	NP_498726.3
	GALC	NP_001037727.1
Mus musculus (house mouse)	Galc	NP_032105.2
Xenopus tropicalis (tropical clawed frog)	galc	NP_001072270.1
Pan troglodytes (chimpanzee)	GALC	XP_001136291.1
Rattus norvegicus (Norway rat)	Galc	NP_001005888.1
Canis lupus familiaris (dog)	GALC	NP_001003238.1
Bos taurus (cattle)	GALC	NP_001192949.1
Gallus gallus (chicken)	GALC	XP_421304.3

Gene Ontology
Bibliography

Related articles in PubMed

The mechanism of glycosphingolipid degradation revealed by a GALC-SapA complex structure.
Hill CH, Cook GM, Spratley SJ, Fawke S, Graham SC, Deane JE
Nature communications9(1)151(2018 Jan)

Expression of individual mutations and haplotypes in the galactocerebrosidase gene identified by the newborn screening program in New York State and in confirmed cases of Krabbe's disease.
Saavedra-Matiz CA, Luzi P, Nichols M, Orsini JJ, Caggana M, Wenger DA
Journal of neuroscience research94(11)1076-83(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the largest expression study of GALC variants/mutations found in Newborn screening and confirmed KD cases.
Title: Expression of individual mutations and haplotypes in the galactocerebrosidase gene identified by the newborn screening program in New York State and in confirmed cases of Krabbe's disease.

This review illustrated The Krabbe's disease is caused by mutation in the enzyme Beta-galactocerebrosidase.
Title: Lysosphingolipids and sphingolipidoses: Psychosine in Krabbe's disease.

Enzyme activities (acid alpha-glucosidase (GAA), galactocerebrosidase (GALC), glucocerebrosidase (GBA), alpha-galactosidase A (GLA), alpha-iduronidase (IDUA) and sphingomyeline phosphodiesterase-1 (SMPD-1)) were measured on ~43,000 de-identified dried blood spot (DBS) punches, and screen positive samples were submitted for DNA sequencing to obtain genotype confirmation of disease risk
Title: Pilot study of newborn screening for six lysosomal storage diseases using Tandem Mass Spectrometry.

Mutations in GALC cause late-onset Krabbe disease with predominant cerebellar ataxia
Title: Mutations in GALC cause late-onset Krabbe disease with predominant cerebellar ataxia.

Case Report: compound GALC heterozygosity in a boy with infantile Krabbe disease with severe clinical course.
Title: Compound Galactosylceramidase Gene (GALC) Heterozygosity in a Boy with Infantile Krabbe Disease (KD).

The following GALC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GALC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22729	NM_000153.3 Latest version!	Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
OHu22526	NM_001201402.1 Latest version!	Homo sapiens galactosylceramidase (GALC), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu23137	NM_001201401.1 Latest version!	Homo sapiens galactosylceramidase (GALC), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu57913	XM_011536618.2 Latest version!	Homo sapiens galactosylceramidase (GALC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu107508	NM_000153.4 Latest version!	Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GALC ( NM_000153.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000144.2 Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22729
Clone ID Related Accession (Same CDS sequence)	NM_000153.3
Accession Version	NM_000153.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	galactocerebrosidase isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA323070.1, D25284.1, D25283.1, BC036518.2, H80485.1, AL136501.4 and BQ015788.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2011 this sequence version replaced NM_000153.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005711.1, SRR1660807.240245.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTATCGTTCT 
CAGATATTGG ATTATCTCTT TAAGCCGAAT TTTGGTGCCT CTTTGCATAT TTTAAAAGTG 
GAAATAGGTG GTGATGGGCA GACAACAGAC GGCACTGAGC CCTCCCACAT GCATTATGCA 
CTAGATGAGA ATTATTTCCG AGGATACGAG TGGTGGTTGA TGAAAGAAGC TAAGAAGAGG 
AATCCCAATA TTACACTCAT TGGGTTGCCA TGGTCATTCC CTGGATGGCT GGGAAAAGGT 
TTCGACTGGC CTTATGTCAA TCTTCAGCTG ACTGCCTATT ATGTCGTGAC CTGGATTGTG 
GGCGCCAAGC GTTACCATGA TTTGGACATT GATTATATTG GAATTTGGAA TGAGAGGTCA 
TATAATGCCA ATTATATTAA GATATTAAGA AAAATGCTGA ATTATCAAGG TCTCCAGCGA 
GTGAAAATCA TAGCAAGTGA TAATCTCTGG GAGTCCATCT CTGCATCCAT GCTCCTTGAT 
GCCGAACTCT TCAAGGTGGT TGATGTTATA GGGGCTCATT ATCCTGGAAC CCATTCAGCA 
AAAGATGCAA AGTTGACTGG GAAGAAGCTT TGGTCTTCTG AAGACTTTAG CACTTTAAAT 
AGTGACATGG GTGCAGGCTG CTGGGGTCGC ATTTTAAATC AGAATTATAT CAATGGCTAT 
ATGACTTCCA CAATCGCATG GAATTTAGTG GCTAGTTACT ATGAACAGTT GCCTTATGGG 
AGATGCGGGT TGATGACGGC CCAGGAGCCA TGGAGTGGGC ACTACGTGGT AGAATCTCCT 
GTCTGGGTAT CAGCTCATAC CACTCAGTTT ACTCAACCTG GCTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CTGATGGCTT AGGGAACCTC 
ACCATCATCA TTGAAACCAT GAGTCATAAA CATTCTAAGT GCATACGGCC ATTTCTTCCT 
TATTTCAATG TGTCACAACA ATTTGCCACC TTTGTTCTTA AGGGATCTTT TAGTGAAATA 
CCAGAGCTAC AGGTATGGTA TACCAAACTT GGAAAAACAT CCGAAAGATT TCTTTTTAAG 
CAGCTGGATT CTCTATGGCT CCTTGACAGC GATGGCAGTT TCACACTGAG CCTGCATGAA 
GATGAGCTGT TCACACTCAC CACTCTCACC ACTGGTCGCA AAGGCAGCTA CCCGCTTCCT 
CCAAAATCCC AGCCCTTCCC AAGTACCTAT AAGGATGATT TCAATGTTGA TTACCCATTT 
TTTAGTGAAG CTCCAAACTT TGCTGATCAA ACTGGTGTAT TTGAATATTT TACAAATATT 
GAAGACCCTG GCGAGCATCA CTTCACGCTA CGCCAAGTTC TCAACCAGAG ACCCATTACG 
TGGGCTGCCG ATGCATCCAA CACAATCAGT ATTATAGGAG ACTACAACTG GACCAATCTG 
ACTATAAAGT GTGATGTATA CATAGAGACC CCTGACACAG GAGGTGTGTT CATTGCAGGA 
AGAGTAAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGAG GAATTTTCTT CTGGATTTTT 
GCAAATGGAT CTTACAGGGT TACAGGTGAT TTAGCTGGAT GGATTATATA TGCTTTAGGA 
CGTGTTGAAG TTACAGCAAA AAAATGGTAT ACACTCACGT TAACTATTAA GGGTCATTTC 
ACCTCTGGCA TGCTGAATGA CAAGTCTCTG TGGACAGACA TCCCTGTGAA TTTTCCAAAG 
AATGGCTGGG CTGCAATTGG AACTCACTCC TTTGAATTTG CACAGTTTGA CAACTTTCTT 
GTGGAAGCCA CACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000144.2
CDS	108..2165
Translation	MAEWLLSASWQRRAKAMTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR

Target ORF information:

RefSeq Version	NM_000153.3
Organism	Homo sapiens(human)
Definition	Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000153.3

ORF Insert Sequence:

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTATCGTTCT 
CAGATATTGG ATTATCTCTT TAAGCCGAAT TTTGGTGCCT CTTTGCATAT TTTAAAAGTG 
GAAATAGGTG GTGATGGGCA GACAACAGAC GGCACTGAGC CCTCCCACAT GCATTATGCA 
CTAGATGAGA ATTATTTCCG AGGATACGAG TGGTGGTTGA TGAAAGAAGC TAAGAAGAGG 
AATCCCAATA TTACACTCAT TGGGTTGCCA TGGTCATTCC CTGGATGGCT GGGAAAAGGT 
TTCGACTGGC CTTATGTCAA TCTTCAGCTG ACTGCCTATT ATGTCGTGAC CTGGATTGTG 
GGCGCCAAGC GTTACCATGA TTTGGACATT GATTATATTG GAATTTGGAA TGAGAGGTCA 
TATAATGCCA ATTATATTAA GATATTAAGA AAAATGCTGA ATTATCAAGG TCTCCAGCGA 
GTGAAAATCA TAGCAAGTGA TAATCTCTGG GAGTCCATCT CTGCATCCAT GCTCCTTGAT 
GCCGAACTCT TCAAGGTGGT TGATGTTATA GGGGCTCATT ATCCTGGAAC CCATTCAGCA 
AAAGATGCAA AGTTGACTGG GAAGAAGCTT TGGTCTTCTG AAGACTTTAG CACTTTAAAT 
AGTGACATGG GTGCAGGCTG CTGGGGTCGC ATTTTAAATC AGAATTATAT CAATGGCTAT 
ATGACTTCCA CAATCGCATG GAATTTAGTG GCTAGTTACT ATGAACAGTT GCCTTATGGG 
AGATGCGGGT TGATGACGGC CCAGGAGCCA TGGAGTGGGC ACTACGTGGT AGAATCTCCT 
GTCTGGGTAT CAGCTCATAC CACTCAGTTT ACTCAACCTG GCTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CTGATGGCTT AGGGAACCTC 
ACCATCATCA TTGAAACCAT GAGTCATAAA CATTCTAAGT GCATACGGCC ATTTCTTCCT 
TATTTCAATG TGTCACAACA ATTTGCCACC TTTGTTCTTA AGGGATCTTT TAGTGAAATA 
CCAGAGCTAC AGGTATGGTA TACCAAACTT GGAAAAACAT CCGAAAGATT TCTTTTTAAG 
CAGCTGGATT CTCTATGGCT CCTTGACAGC GATGGCAGTT TCACACTGAG CCTGCATGAA 
GATGAGCTGT TCACACTCAC CACTCTCACC ACTGGTCGCA AAGGCAGCTA CCCGCTTCCT 
CCAAAATCCC AGCCCTTCCC AAGTACCTAT AAGGATGATT TCAATGTTGA TTACCCATTT 
TTTAGTGAAG CTCCAAACTT TGCTGATCAA ACTGGTGTAT TTGAATATTT TACAAATATT 
GAAGACCCTG GCGAGCATCA CTTCACGCTA CGCCAAGTTC TCAACCAGAG ACCCATTACG 
TGGGCTGCCG ATGCATCCAA CACAATCAGT ATTATAGGAG ACTACAACTG GACCAATCTG 
ACTATAAAGT GTGATGTATA CATAGAGACC CCTGACACAG GAGGTGTGTT CATTGCAGGA 
AGAGTAAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGAG GAATTTTCTT CTGGATTTTT 
GCAAATGGAT CTTACAGGGT TACAGGTGAT TTAGCTGGAT GGATTATATA TGCTTTAGGA 
CGTGTTGAAG TTACAGCAAA AAAATGGTAT ACACTCACGT TAACTATTAA GGGTCATTTC 
ACCTCTGGCA TGCTGAATGA CAAGTCTCTG TGGACAGACA TCCCTGTGAA TTTTCCAAAG 
AATGGCTGGG CTGCAATTGG AACTCACTCC TTTGAATTTG CACAGTTTGA CAACTTTCTT 
GTGGAAGCCA CACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( NM_001201402.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188331.1 Homo sapiens galactosylceramidase (GALC), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22526
Clone ID Related Accession (Same CDS sequence)	NM_001201402.1
Accession Version	NM_001201402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	galactocerebrosidase isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334180.1, D25283.1, BC036518.2, H80485.1, AL136501.4 and BQ015788.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.277084.1, SRR1660807.45467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGGGA AGAGCCATGG TAGGGCCACT CATGGCCCTC TTCCTTTGGC GGACCTTGGA 
ATCCACCTTC CCTGCGTTAA AGTGCTCCAC CAGGTGACGC CGGAAGAGAA GCCAGCAGCA 
ACCTCCCGAC TTCTAGTAAA TTACCCAGAG CCCTATCGTT CTCAGATATT GGATTATCTC 
TTTAAGCCGA ATTTTGGTGC CTCTTTGCAT ATTTTAAAAG TGGAAATAGG TGGTGATGGG 
CAGACAACAG ACGGCACTGA GCCCTCCCAC ATGCATTATG CACTAGATGA GAATTATTTC 
CGAGGATACG AGTGGTGGTT GATGAAAGAA GCTAAGAAGA GGAATCCCAA TATTACACTC 
ATTGGGTTGC CATGGTCATT CCCTGGATGG CTGGGAAAAG GTTTCGACTG GCCTTATGTC 
AATCTTCAGC TGACTGCCTA TTATGTCGTG ACCTGGATTG TGGGCGCCAA GCGTTACCAT 
GATTTGGACA TTGATTATAT TGGAATTTGG AATGAGAGGT CATATAATGC CAATTATATT 
AAGATATTAA GAAAAATGCT GAATTATCAA GGTCTCCAGC GAGTGAAAAT CATAGCAAGT 
GATAATCTCT GGGAGTCCAT CTCTGCATCC ATGCTCCTTG ATGCCGAACT CTTCAAGGTG 
GTTGATGTTA TAGGGGCTCA TTATCCTGGA ACCCATTCAG CAAAAGATGC AAAGTTGACT 
GGGAAGAAGC TTTGGTCTTC TGAAGACTTT AGCACTTTAA ATAGTGACAT GGGTGCAGGC 
TGCTGGGGTC GCATTTTAAA TCAGAATTAT ATCAATGGCT ATATGACTTC CACAATCGCA 
TGGAATTTAG TGGCTAGTTA CTATGAACAG TTGCCTTATG GGAGATGCGG GTTGATGACG 
GCCCAGGAGC CATGGAGTGG GCACTACGTG GTAGAATCTC CTGTCTGGGT ATCAGCTCAT 
ACCACTCAGT TTACTCAACC TGGCTGGTAT TACCTGAAGA CAGTTGGCCA TTTAGAGAAA 
GGAGGAAGCT ACGTAGCTCT GACTGATGGC TTAGGGAACC TCACCATCAT CATTGAAACC 
ATGAGTCATA AACATTCTAA GTGCATACGG CCATTTCTTC CTTATTTCAA TGTGTCACAA 
CAATTTGCCA CCTTTGTTCT TAAGGGATCT TTTAGTGAAA TACCAGAGCT ACAGGTATGG 
TATACCAAAC TTGGAAAAAC ATCCGAAAGA TTTCTTTTTA AGCAGCTGGA TTCTCTATGG 
CTCCTTGACA GCGATGGCAG TTTCACACTG AGCCTGCATG AAGATGAGCT GTTCACACTC 
ACCACTCTCA CCACTGGTCG CAAAGGCAGC TACCCGCTTC CTCCAAAATC CCAGCCCTTC 
CCAAGTACCT ATAAGGATGA TTTCAATGTT GATTACCCAT TTTTTAGTGA AGCTCCAAAC 
TTTGCTGATC AAACTGGTGT ATTTGAATAT TTTACAAATA TTGAAGACCC TGGCGAGCAT 
CACTTCACGC TACGCCAAGT TCTCAACCAG AGACCCATTA CGTGGGCTGC CGATGCATCC 
AACACAATCA GTATTATAGG AGACTACAAC TGGACCAATC TGACTATAAA GTGTGATGTA 
TACATAGAGA CCCCTGACAC AGGAGGTGTG TTCATTGCAG GAAGAGTAAA TAAAGGTGGT 
ATTTTGATTA GAAGTGCCAG AGGAATTTTC TTCTGGATTT TTGCAAATGG ATCTTACAGG 
GTTACAGGTG ATTTAGCTGG ATGGATTATA TATGCTTTAG GACGTGTTGA AGTTACAGCA 
AAAAAATGGT ATACACTCAC GTTAACTATT AAGGGTCATT TCACCTCTGG CATGCTGAAT 
GACAAGTCTC TGTGGACAGA CATCCCTGTG AATTTTCCAA AGAATGGCTG GGCTGCAATT 
GGAACTCACT CCTTTGAATT TGCACAGTTT GACAACTTTC TTGTGGAAGC CACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188331.1
CDS	167..2146
Translation	MLGKSHGRATHGPLPLADLGIHLPCVKVLHQVTPEEKPAATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR

Target ORF information:

RefSeq Version	NM_001201402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens galactosylceramidase (GALC), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201402.1

ORF Insert Sequence:

ATGCTGGGGA AGAGCCATGG TAGGGCCACT CATGGCCCTC TTCCTTTGGC GGACCTTGGA 
ATCCACCTTC CCTGCGTTAA AGTGCTCCAC CAGGTGACGC CGGAAGAGAA GCCAGCAGCA 
ACCTCCCGAC TTCTAGTAAA TTACCCAGAG CCCTATCGTT CTCAGATATT GGATTATCTC 
TTTAAGCCGA ATTTTGGTGC CTCTTTGCAT ATTTTAAAAG TGGAAATAGG TGGTGATGGG 
CAGACAACAG ACGGCACTGA GCCCTCCCAC ATGCATTATG CACTAGATGA GAATTATTTC 
CGAGGATACG AGTGGTGGTT GATGAAAGAA GCTAAGAAGA GGAATCCCAA TATTACACTC 
ATTGGGTTGC CATGGTCATT CCCTGGATGG CTGGGAAAAG GTTTCGACTG GCCTTATGTC 
AATCTTCAGC TGACTGCCTA TTATGTCGTG ACCTGGATTG TGGGCGCCAA GCGTTACCAT 
GATTTGGACA TTGATTATAT TGGAATTTGG AATGAGAGGT CATATAATGC CAATTATATT 
AAGATATTAA GAAAAATGCT GAATTATCAA GGTCTCCAGC GAGTGAAAAT CATAGCAAGT 
GATAATCTCT GGGAGTCCAT CTCTGCATCC ATGCTCCTTG ATGCCGAACT CTTCAAGGTG 
GTTGATGTTA TAGGGGCTCA TTATCCTGGA ACCCATTCAG CAAAAGATGC AAAGTTGACT 
GGGAAGAAGC TTTGGTCTTC TGAAGACTTT AGCACTTTAA ATAGTGACAT GGGTGCAGGC 
TGCTGGGGTC GCATTTTAAA TCAGAATTAT ATCAATGGCT ATATGACTTC CACAATCGCA 
TGGAATTTAG TGGCTAGTTA CTATGAACAG TTGCCTTATG GGAGATGCGG GTTGATGACG 
GCCCAGGAGC CATGGAGTGG GCACTACGTG GTAGAATCTC CTGTCTGGGT ATCAGCTCAT 
ACCACTCAGT TTACTCAACC TGGCTGGTAT TACCTGAAGA CAGTTGGCCA TTTAGAGAAA 
GGAGGAAGCT ACGTAGCTCT GACTGATGGC TTAGGGAACC TCACCATCAT CATTGAAACC 
ATGAGTCATA AACATTCTAA GTGCATACGG CCATTTCTTC CTTATTTCAA TGTGTCACAA 
CAATTTGCCA CCTTTGTTCT TAAGGGATCT TTTAGTGAAA TACCAGAGCT ACAGGTATGG 
TATACCAAAC TTGGAAAAAC ATCCGAAAGA TTTCTTTTTA AGCAGCTGGA TTCTCTATGG 
CTCCTTGACA GCGATGGCAG TTTCACACTG AGCCTGCATG AAGATGAGCT GTTCACACTC 
ACCACTCTCA CCACTGGTCG CAAAGGCAGC TACCCGCTTC CTCCAAAATC CCAGCCCTTC 
CCAAGTACCT ATAAGGATGA TTTCAATGTT GATTACCCAT TTTTTAGTGA AGCTCCAAAC 
TTTGCTGATC AAACTGGTGT ATTTGAATAT TTTACAAATA TTGAAGACCC TGGCGAGCAT 
CACTTCACGC TACGCCAAGT TCTCAACCAG AGACCCATTA CGTGGGCTGC CGATGCATCC 
AACACAATCA GTATTATAGG AGACTACAAC TGGACCAATC TGACTATAAA GTGTGATGTA 
TACATAGAGA CCCCTGACAC AGGAGGTGTG TTCATTGCAG GAAGAGTAAA TAAAGGTGGT 
ATTTTGATTA GAAGTGCCAG AGGAATTTTC TTCTGGATTT TTGCAAATGG ATCTTACAGG 
GTTACAGGTG ATTTAGCTGG ATGGATTATA TATGCTTTAG GACGTGTTGA AGTTACAGCA 
AAAAAATGGT ATACACTCAC GTTAACTATT AAGGGTCATT TCACCTCTGG CATGCTGAAT 
GACAAGTCTC TGTGGACAGA CATCCCTGTG AATTTTCCAA AGAATGGCTG GGCTGCAATT 
GGAACTCACT CCTTTGAATT TGCACAGTTT GACAACTTTC TTGTGGAAGC CACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( NM_001201401.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188330.1 Homo sapiens galactosylceramidase (GALC), transcript variant 3, mRNA.

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CloneID	OHu23137
Clone ID Related Accession (Same CDS sequence)	NM_001201401.1
Accession Version	NM_001201401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	galactocerebrosidase isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA323070.1, D25284.1, D25283.1, BC036518.2, H80485.1, AL136501.4 and BQ015788.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.254083.1, SRR1803617.139324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGCCGAA TTTTGGTGCC TCTTTGCATA TTTTAAAAGT GGAAATAGGT 
GGTGATGGGC AGACAACAGA CGGCACTGAG CCCTCCCACA TGCATTATGC ACTAGATGAG 
AATTATTTCC GAGGATACGA GTGGTGGTTG ATGAAAGAAG CTAAGAAGAG GAATCCCAAT 
ATTACACTCA TTGGGTTGCC ATGGTCATTC CCTGGATGGC TGGGAAAAGG TTTCGACTGG 
CCTTATGTCA ATCTTCAGCT GACTGCCTAT TATGTCGTGA CCTGGATTGT GGGCGCCAAG 
CGTTACCATG ATTTGGACAT TGATTATATT GGAATTTGGA ATGAGAGGTC ATATAATGCC 
AATTATATTA AGATATTAAG AAAAATGCTG AATTATCAAG GTCTCCAGCG AGTGAAAATC 
ATAGCAAGTG ATAATCTCTG GGAGTCCATC TCTGCATCCA TGCTCCTTGA TGCCGAACTC 
TTCAAGGTGG TTGATGTTAT AGGGGCTCAT TATCCTGGAA CCCATTCAGC AAAAGATGCA 
AAGTTGACTG GGAAGAAGCT TTGGTCTTCT GAAGACTTTA GCACTTTAAA TAGTGACATG 
GGTGCAGGCT GCTGGGGTCG CATTTTAAAT CAGAATTATA TCAATGGCTA TATGACTTCC 
ACAATCGCAT GGAATTTAGT GGCTAGTTAC TATGAACAGT TGCCTTATGG GAGATGCGGG 
TTGATGACGG CCCAGGAGCC ATGGAGTGGG CACTACGTGG TAGAATCTCC TGTCTGGGTA 
TCAGCTCATA CCACTCAGTT TACTCAACCT GGCTGGTATT ACCTGAAGAC AGTTGGCCAT 
TTAGAGAAAG GAGGAAGCTA CGTAGCTCTG ACTGATGGCT TAGGGAACCT CACCATCATC 
ATTGAAACCA TGAGTCATAA ACATTCTAAG TGCATACGGC CATTTCTTCC TTATTTCAAT 
GTGTCACAAC AATTTGCCAC CTTTGTTCTT AAGGGATCTT TTAGTGAAAT ACCAGAGCTA 
CAGGTATGGT ATACCAAACT TGGAAAAACA TCCGAAAGAT TTCTTTTTAA GCAGCTGGAT 
TCTCTATGGC TCCTTGACAG CGATGGCAGT TTCACACTGA GCCTGCATGA AGATGAGCTG 
TTCACACTCA CCACTCTCAC CACTGGTCGC AAAGGCAGCT ACCCGCTTCC TCCAAAATCC 
CAGCCCTTCC CAAGTACCTA TAAGGATGAT TTCAATGTTG ATTACCCATT TTTTAGTGAA 
GCTCCAAACT TTGCTGATCA AACTGGTGTA TTTGAATATT TTACAAATAT TGAAGACCCT 
GGCGAGCATC ACTTCACGCT ACGCCAAGTT CTCAACCAGA GACCCATTAC GTGGGCTGCC 
GATGCATCCA ACACAATCAG TATTATAGGA GACTACAACT GGACCAATCT GACTATAAAG 
TGTGATGTAT ACATAGAGAC CCCTGACACA GGAGGTGTGT TCATTGCAGG AAGAGTAAAT 
AAAGGTGGTA TTTTGATTAG AAGTGCCAGA GGAATTTTCT TCTGGATTTT TGCAAATGGA 
TCTTACAGGG TTACAGGTGA TTTAGCTGGA TGGATTATAT ATGCTTTAGG ACGTGTTGAA 
GTTACAGCAA AAAAATGGTA TACACTCACG TTAACTATTA AGGGTCATTT CACCTCTGGC 
ATGCTGAATG ACAAGTCTCT GTGGACAGAC ATCCCTGTGA ATTTTCCAAA GAATGGCTGG 
GCTGCAATTG GAACTCACTC CTTTGAATTT GCACAGTTTG ACAACTTTCT TGTGGAAGCC 
ACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188330.1
CDS	108..2096
Translation	MAEWLLSASWQRRAKAMTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGAVSGGGPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR

Target ORF information:

RefSeq Version	NM_001201401.1
Organism	Homo sapiens(human)
Definition	Homo sapiens galactosylceramidase (GALC), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201401.1

ORF Insert Sequence:

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGCCGAA TTTTGGTGCC TCTTTGCATA TTTTAAAAGT GGAAATAGGT 
GGTGATGGGC AGACAACAGA CGGCACTGAG CCCTCCCACA TGCATTATGC ACTAGATGAG 
AATTATTTCC GAGGATACGA GTGGTGGTTG ATGAAAGAAG CTAAGAAGAG GAATCCCAAT 
ATTACACTCA TTGGGTTGCC ATGGTCATTC CCTGGATGGC TGGGAAAAGG TTTCGACTGG 
CCTTATGTCA ATCTTCAGCT GACTGCCTAT TATGTCGTGA CCTGGATTGT GGGCGCCAAG 
CGTTACCATG ATTTGGACAT TGATTATATT GGAATTTGGA ATGAGAGGTC ATATAATGCC 
AATTATATTA AGATATTAAG AAAAATGCTG AATTATCAAG GTCTCCAGCG AGTGAAAATC 
ATAGCAAGTG ATAATCTCTG GGAGTCCATC TCTGCATCCA TGCTCCTTGA TGCCGAACTC 
TTCAAGGTGG TTGATGTTAT AGGGGCTCAT TATCCTGGAA CCCATTCAGC AAAAGATGCA 
AAGTTGACTG GGAAGAAGCT TTGGTCTTCT GAAGACTTTA GCACTTTAAA TAGTGACATG 
GGTGCAGGCT GCTGGGGTCG CATTTTAAAT CAGAATTATA TCAATGGCTA TATGACTTCC 
ACAATCGCAT GGAATTTAGT GGCTAGTTAC TATGAACAGT TGCCTTATGG GAGATGCGGG 
TTGATGACGG CCCAGGAGCC ATGGAGTGGG CACTACGTGG TAGAATCTCC TGTCTGGGTA 
TCAGCTCATA CCACTCAGTT TACTCAACCT GGCTGGTATT ACCTGAAGAC AGTTGGCCAT 
TTAGAGAAAG GAGGAAGCTA CGTAGCTCTG ACTGATGGCT TAGGGAACCT CACCATCATC 
ATTGAAACCA TGAGTCATAA ACATTCTAAG TGCATACGGC CATTTCTTCC TTATTTCAAT 
GTGTCACAAC AATTTGCCAC CTTTGTTCTT AAGGGATCTT TTAGTGAAAT ACCAGAGCTA 
CAGGTATGGT ATACCAAACT TGGAAAAACA TCCGAAAGAT TTCTTTTTAA GCAGCTGGAT 
TCTCTATGGC TCCTTGACAG CGATGGCAGT TTCACACTGA GCCTGCATGA AGATGAGCTG 
TTCACACTCA CCACTCTCAC CACTGGTCGC AAAGGCAGCT ACCCGCTTCC TCCAAAATCC 
CAGCCCTTCC CAAGTACCTA TAAGGATGAT TTCAATGTTG ATTACCCATT TTTTAGTGAA 
GCTCCAAACT TTGCTGATCA AACTGGTGTA TTTGAATATT TTACAAATAT TGAAGACCCT 
GGCGAGCATC ACTTCACGCT ACGCCAAGTT CTCAACCAGA GACCCATTAC GTGGGCTGCC 
GATGCATCCA ACACAATCAG TATTATAGGA GACTACAACT GGACCAATCT GACTATAAAG 
TGTGATGTAT ACATAGAGAC CCCTGACACA GGAGGTGTGT TCATTGCAGG AAGAGTAAAT 
AAAGGTGGTA TTTTGATTAG AAGTGCCAGA GGAATTTTCT TCTGGATTTT TGCAAATGGA 
TCTTACAGGG TTACAGGTGA TTTAGCTGGA TGGATTATAT ATGCTTTAGG ACGTGTTGAA 
GTTACAGCAA AAAAATGGTA TACACTCACG TTAACTATTA AGGGTCATTT CACCTCTGGC 
ATGCTGAATG ACAAGTCTCT GTGGACAGAC ATCCCTGTGA ATTTTCCAAA GAATGGCTGG 
GCTGCAATTG GAACTCACTC CTTTGAATTT GCACAGTTTG ACAACTTTCT TGTGGAAGCC 
ACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_011536618.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534920.1 Homo sapiens galactosylceramidase (GALC), transcript variant X1, mRNA.

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CloneID	OHu57913
Clone ID Related Accession (Same CDS sequence)	XM_011536618.2
Accession Version	XM_011536618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	galactocerebrosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCA TGGTGGCCGA CCTTTGGGCA ACCTCCCGAC TTCTAGTAAA TTACCCAGAG 
CCCTATCGTT CTCAGATATT GGATTATCTC TTTAAGCCGA ATTTTGGTGC CTCTTTGCAT 
ATTTTAAAAG TGGAAATAGG TGGTGATGGG CAGACAACAG ACGGCACTGA GCCCTCCCAC 
ATGCATTATG CACTAGATGA GAATTATTTC CGAGGATACG AGTGGTGGTT GATGAAAGAA 
GCTAAGAAGA GGAATCCCAA TATTACACTC ATTGGGTTGC CATGGTCATT CCCTGGATGG 
CTGGGAAAAG GTTTCGACTG GCCTTATGTC AATCTTCAGC TGACTGCCTA TTATGTCGTG 
ACCTGGATTG TGGGCGCCAA GCGTTACCAT GATTTGGACA TTGATTATAT TGGAATTTGG 
AATGAGAGGT CATATAATGC CAATTATATT AAGATATTAA GAAAAATGCT GAATTATCAA 
GGTCTCCAGC GAGTGAAAAT CATAGCAAGT GATAATCTCT GGGAGTCCAT CTCTGCATCC 
ATGCTCCTTG ATGCCGAACT CTTCAAGGTG GTTGATGTTA TAGGGGCTCA TTATCCTGGA 
ACCCATTCAG CAAAAGATGC AAAGTTGACT GGGAAGAAGC TTTGGTCTTC TGAAGACTTT 
AGCACTTTAA ATAGTGACAT GGGTGCAGGC TGCTGGGGTC GCATTTTAAA TCAGAATTAT 
ATCAATGGCT ATATGACTTC CACAATCGCA TGGAATTTAG TGGCTAGTTA CTATGAACAG 
TTGCCTTATG GGAGATGCGG GTTGATGACG GCCCAGGAGC CATGGAGTGG GCACTACGTG 
GTAGAATCTC CTGTCTGGGT ATCAGCTCAT ACCACTCAGT TTACTCAACC TGGCTGGTAT 
TACCTGAAGA CAGTTGGCCA TTTAGAGAAA GGAGGAAGCT ACGTAGCTCT GACTGATGGC 
TTAGGGAACC TCACCATCAT CATTGAAACC ATGAGTCATA AACATTCTAA GTGCATACGG 
CCATTTCTTC CTTATTTCAA TGTGTCACAA CAATTTGCCA CCTTTGTTCT TAAGGGATCT 
TTTAGTGAAA TACCAGAGCT ACAGGTATGG TATACCAAAC TTGGAAAAAC ATCCGAAAGA 
TTTCTTTTTA AGCAGCTGGA TTCTCTATGG CTCCTTGACA GCGATGGCAG TTTCACACTG 
AGCCTGCATG AAGATGAGCT GTTCACACTC ACCACTCTCA CCACTGGTCG CAAAGGCAGC 
TACCCGCTTC CTCCAAAATC CCAGCCCTTC CCAAGTACCT ATAAGGATGA TTTCAATGTT 
GATTACCCAT TTTTTAGTGA AGCTCCAAAC TTTGCTGATC AAACTGGTGT ATTTGAATAT 
TTTACAAATA TTGAAGACCC TGGCGAGCAT CACTTCACGC TACGCCAAGT TCTCAACCAG 
AGACCCATTA CATGGGCTGC CGATGCATCC AACACAATCA GTATTATAGG AGACTACAAC 
TGGACCAATC TGACTATAAA GTGTGATGTA TACATAGAGA CCCCTGACAC AGGAGGTGTG 
TTCATTGCAG GAAGAGTAAA TAAAGGTGGT ATTTTGATTA GAAGTGCCAG AGGAATTTTC 
TTCTGGATTT TTGCAAATGG ATCTTACAGG GTTACAGGTG ATTTAGCTGG ATGGATTATA 
TATGCTTTAG GACGTGTTGA AGTTACAGCA AAAAAATGGT ATACACTCAC GTTAACTATT 
AAGGGTCATT TCACCTCTGG CATGCTGAAT GACAAGTCTC TGTGGACAGA CATCCCTGTG 
AATTTTCCAA AGAATGGCTG GGCTGCAATT GGAACTCACT CCTTTGAATT TGCACAGTTT 
GACAACTTTC TTGTGGAAGC CACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534920.1
CDS	558..2447
Translation	MGFMVADLWATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR

Target ORF information:

RefSeq Version	XM_011536618.2
Organism	Homo sapiens(human)
Definition	Homo sapiens galactosylceramidase (GALC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536618.2

ORF Insert Sequence:

ATGGGCTTCA TGGTGGCCGA CCTTTGGGCA ACCTCCCGAC TTCTAGTAAA TTACCCAGAG 
CCCTATCGTT CTCAGATATT GGATTATCTC TTTAAGCCGA ATTTTGGTGC CTCTTTGCAT 
ATTTTAAAAG TGGAAATAGG TGGTGATGGG CAGACAACAG ACGGCACTGA GCCCTCCCAC 
ATGCATTATG CACTAGATGA GAATTATTTC CGAGGATACG AGTGGTGGTT GATGAAAGAA 
GCTAAGAAGA GGAATCCCAA TATTACACTC ATTGGGTTGC CATGGTCATT CCCTGGATGG 
CTGGGAAAAG GTTTCGACTG GCCTTATGTC AATCTTCAGC TGACTGCCTA TTATGTCGTG 
ACCTGGATTG TGGGCGCCAA GCGTTACCAT GATTTGGACA TTGATTATAT TGGAATTTGG 
AATGAGAGGT CATATAATGC CAATTATATT AAGATATTAA GAAAAATGCT GAATTATCAA 
GGTCTCCAGC GAGTGAAAAT CATAGCAAGT GATAATCTCT GGGAGTCCAT CTCTGCATCC 
ATGCTCCTTG ATGCCGAACT CTTCAAGGTG GTTGATGTTA TAGGGGCTCA TTATCCTGGA 
ACCCATTCAG CAAAAGATGC AAAGTTGACT GGGAAGAAGC TTTGGTCTTC TGAAGACTTT 
AGCACTTTAA ATAGTGACAT GGGTGCAGGC TGCTGGGGTC GCATTTTAAA TCAGAATTAT 
ATCAATGGCT ATATGACTTC CACAATCGCA TGGAATTTAG TGGCTAGTTA CTATGAACAG 
TTGCCTTATG GGAGATGCGG GTTGATGACG GCCCAGGAGC CATGGAGTGG GCACTACGTG 
GTAGAATCTC CTGTCTGGGT ATCAGCTCAT ACCACTCAGT TTACTCAACC TGGCTGGTAT 
TACCTGAAGA CAGTTGGCCA TTTAGAGAAA GGAGGAAGCT ACGTAGCTCT GACTGATGGC 
TTAGGGAACC TCACCATCAT CATTGAAACC ATGAGTCATA AACATTCTAA GTGCATACGG 
CCATTTCTTC CTTATTTCAA TGTGTCACAA CAATTTGCCA CCTTTGTTCT TAAGGGATCT 
TTTAGTGAAA TACCAGAGCT ACAGGTATGG TATACCAAAC TTGGAAAAAC ATCCGAAAGA 
TTTCTTTTTA AGCAGCTGGA TTCTCTATGG CTCCTTGACA GCGATGGCAG TTTCACACTG 
AGCCTGCATG AAGATGAGCT GTTCACACTC ACCACTCTCA CCACTGGTCG CAAAGGCAGC 
TACCCGCTTC CTCCAAAATC CCAGCCCTTC CCAAGTACCT ATAAGGATGA TTTCAATGTT 
GATTACCCAT TTTTTAGTGA AGCTCCAAAC TTTGCTGATC AAACTGGTGT ATTTGAATAT 
TTTACAAATA TTGAAGACCC TGGCGAGCAT CACTTCACGC TACGCCAAGT TCTCAACCAG 
AGACCCATTA CATGGGCTGC CGATGCATCC AACACAATCA GTATTATAGG AGACTACAAC 
TGGACCAATC TGACTATAAA GTGTGATGTA TACATAGAGA CCCCTGACAC AGGAGGTGTG 
TTCATTGCAG GAAGAGTAAA TAAAGGTGGT ATTTTGATTA GAAGTGCCAG AGGAATTTTC 
TTCTGGATTT TTGCAAATGG ATCTTACAGG GTTACAGGTG ATTTAGCTGG ATGGATTATA 
TATGCTTTAG GACGTGTTGA AGTTACAGCA AAAAAATGGT ATACACTCAC GTTAACTATT 
AAGGGTCATT TCACCTCTGG CATGCTGAAT GACAAGTCTC TGTGGACAGA CATCCCTGTG 
AATTTTCCAA AGAATGGCTG GGCTGCAATT GGAACTCACT CCTTTGAATT TGCACAGTTT 
GACAACTTTC TTGTGGAAGC CACACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( NM_000153.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000144.2 Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.

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CloneID	OHu107508
Clone ID Related Accession (Same CDS sequence)	NM_000153.4
Accession Version	NM_000153.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	galactocerebrosidase isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157955.5 and AL136501.4. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000153.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005711.1, HM005712.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261304.7/ ENSP00000261304.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTATCGTTCT 
CAGATATTGG ATTATCTCTT TAAGCCGAAT TTTGGTGCCT CTTTGCATAT TTTAAAAGTG 
GAAATAGGTG GTGATGGGCA GACAACAGAC GGCACTGAGC CCTCCCACAT GCATTATGCA 
CTAGATGAGA ATTATTTCCG AGGATACGAG TGGTGGTTGA TGAAAGAAGC TAAGAAGAGG 
AATCCCAATA TTACACTCAT TGGGTTGCCA TGGTCATTCC CTGGATGGCT GGGAAAAGGT 
TTCGACTGGC CTTATGTCAA TCTTCAGCTG ACTGCCTATT ATGTCGTGAC CTGGATTGTG 
GGCGCCAAGC GTTACCATGA TTTGGACATT GATTATATTG GAATTTGGAA TGAGAGGTCA 
TATAATGCCA ATTATATTAA GATATTAAGA AAAATGCTGA ATTATCAAGG TCTCCAGCGA 
GTGAAAATCA TAGCAAGTGA TAATCTCTGG GAGTCCATCT CTGCATCCAT GCTCCTTGAT 
GCCGAACTCT TCAAGGTGGT TGATGTTATA GGGGCTCATT ATCCTGGAAC CCATTCAGCA 
AAAGATGCAA AGTTGACTGG GAAGAAGCTT TGGTCTTCTG AAGACTTTAG CACTTTAAAT 
AGTGACATGG GTGCAGGCTG CTGGGGTCGC ATTTTAAATC AGAATTATAT CAATGGCTAT 
ATGACTTCCA CAATCGCATG GAATTTAGTG GCTAGTTACT ATGAACAGTT GCCTTATGGG 
AGATGCGGGT TGATGACGGC CCAGGAGCCA TGGAGTGGGC ACTACGTGGT AGAATCTCCT 
GTCTGGGTAT CAGCTCATAC CACTCAGTTT ACTCAACCTG GCTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CTGATGGCTT AGGGAACCTC 
ACCATCATCA TTGAAACCAT GAGTCATAAA CATTCTAAGT GCATACGGCC ATTTCTTCCT 
TATTTCAATG TGTCACAACA ATTTGCCACC TTTGTTCTTA AGGGATCTTT TAGTGAAATA 
CCAGAGCTAC AGGTATGGTA TACCAAACTT GGAAAAACAT CCGAAAGATT TCTTTTTAAG 
CAGCTGGATT CTCTATGGCT CCTTGACAGC GATGGCAGTT TCACACTGAG CCTGCATGAA 
GATGAGCTGT TCACACTCAC CACTCTCACC ACTGGTCGCA AAGGCAGCTA CCCGCTTCCT 
CCAAAATCCC AGCCCTTCCC AAGTACCTAT AAGGATGATT TCAATGTTGA TTACCCATTT 
TTTAGTGAAG CTCCAAACTT TGCTGATCAA ACTGGTGTAT TTGAATATTT TACAAATATT 
GAAGACCCTG GCGAGCATCA CTTCACGCTA CGCCAAGTTC TCAACCAGAG ACCCATTACA 
TGGGCTGCCG ATGCATCCAA CACAATCAGT ATTATAGGAG ACTACAACTG GACCAATCTG 
ACTATAAAGT GTGATGTATA CATAGAGACC CCTGACACAG GAGGTGTGTT CATTGCAGGA 
AGAGTAAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGAG GAATTTTCTT CTGGATTTTT 
GCAAATGGAT CTTACAGGGT TACAGGTGAT TTAGCTGGAT GGATTATATA TGCTTTAGGA 
CGTGTTGAAG TTACAGCAAA AAAATGGTAT ACACTCACGT TAACTATTAA GGGTCATTTC 
ACCTCTGGCA TGCTGAATGA CAAGTCTCTG TGGACAGACA TCCCTGTGAA TTTTCCAAAG 
AATGGCTGGG CTGCAATTGG AACTCACTCC TTTGAATTTG CACAGTTTGA CAACTTTCTT 
GTGGAAGCCA CACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000144.2
CDS	19..2076
Translation	MAEWLLSASWQRRAKAMTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR

Target ORF information:

RefSeq Version	NM_000153.4
Organism	Homo sapiens(human)
Definition	Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000153.4

ORF Insert Sequence:

ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC 
GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC 
GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTATCGTTCT 
CAGATATTGG ATTATCTCTT TAAGCCGAAT TTTGGTGCCT CTTTGCATAT TTTAAAAGTG 
GAAATAGGTG GTGATGGGCA GACAACAGAC GGCACTGAGC CCTCCCACAT GCATTATGCA 
CTAGATGAGA ATTATTTCCG AGGATACGAG TGGTGGTTGA TGAAAGAAGC TAAGAAGAGG 
AATCCCAATA TTACACTCAT TGGGTTGCCA TGGTCATTCC CTGGATGGCT GGGAAAAGGT 
TTCGACTGGC CTTATGTCAA TCTTCAGCTG ACTGCCTATT ATGTCGTGAC CTGGATTGTG 
GGCGCCAAGC GTTACCATGA TTTGGACATT GATTATATTG GAATTTGGAA TGAGAGGTCA 
TATAATGCCA ATTATATTAA GATATTAAGA AAAATGCTGA ATTATCAAGG TCTCCAGCGA 
GTGAAAATCA TAGCAAGTGA TAATCTCTGG GAGTCCATCT CTGCATCCAT GCTCCTTGAT 
GCCGAACTCT TCAAGGTGGT TGATGTTATA GGGGCTCATT ATCCTGGAAC CCATTCAGCA 
AAAGATGCAA AGTTGACTGG GAAGAAGCTT TGGTCTTCTG AAGACTTTAG CACTTTAAAT 
AGTGACATGG GTGCAGGCTG CTGGGGTCGC ATTTTAAATC AGAATTATAT CAATGGCTAT 
ATGACTTCCA CAATCGCATG GAATTTAGTG GCTAGTTACT ATGAACAGTT GCCTTATGGG 
AGATGCGGGT TGATGACGGC CCAGGAGCCA TGGAGTGGGC ACTACGTGGT AGAATCTCCT 
GTCTGGGTAT CAGCTCATAC CACTCAGTTT ACTCAACCTG GCTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CTGATGGCTT AGGGAACCTC 
ACCATCATCA TTGAAACCAT GAGTCATAAA CATTCTAAGT GCATACGGCC ATTTCTTCCT 
TATTTCAATG TGTCACAACA ATTTGCCACC TTTGTTCTTA AGGGATCTTT TAGTGAAATA 
CCAGAGCTAC AGGTATGGTA TACCAAACTT GGAAAAACAT CCGAAAGATT TCTTTTTAAG 
CAGCTGGATT CTCTATGGCT CCTTGACAGC GATGGCAGTT TCACACTGAG CCTGCATGAA 
GATGAGCTGT TCACACTCAC CACTCTCACC ACTGGTCGCA AAGGCAGCTA CCCGCTTCCT 
CCAAAATCCC AGCCCTTCCC AAGTACCTAT AAGGATGATT TCAATGTTGA TTACCCATTT 
TTTAGTGAAG CTCCAAACTT TGCTGATCAA ACTGGTGTAT TTGAATATTT TACAAATATT 
GAAGACCCTG GCGAGCATCA CTTCACGCTA CGCCAAGTTC TCAACCAGAG ACCCATTACA 
TGGGCTGCCG ATGCATCCAA CACAATCAGT ATTATAGGAG ACTACAACTG GACCAATCTG 
ACTATAAAGT GTGATGTATA CATAGAGACC CCTGACACAG GAGGTGTGTT CATTGCAGGA 
AGAGTAAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGAG GAATTTTCTT CTGGATTTTT 
GCAAATGGAT CTTACAGGGT TACAGGTGAT TTAGCTGGAT GGATTATATA TGCTTTAGGA 
CGTGTTGAAG TTACAGCAAA AAAATGGTAT ACACTCACGT TAACTATTAA GGGTCATTTC 
ACCTCTGGCA TGCTGAATGA CAAGTCTCTG TGGACAGACA TCCCTGTGAA TTTTCCAAAG 
AATGGCTGGG CTGCAATTGG AACTCACTCC TTTGAATTTG CACAGTTTGA CAACTTTCTT 
GTGGAAGCCA CACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mechanism of glycosphingolipid degradation revealed by a GALC-SapA complex structure.
Nature communications9(1)151(2018 Jan)
Hill CH,Cook GM,Spratley SJ,Fawke S,Graham SC,Deane JE

Expression of individual mutations and haplotypes in the galactocerebrosidase gene identified by the newborn screening program in New York State and in confirmed cases of Krabbe's disease.
Journal of neuroscience research94(11)1076-83(2016 11)
Saavedra-Matiz CA,Luzi P,Nichols M,Orsini JJ,Caggana M,Wenger DA

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GALC cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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