CBY1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CBY1
Entrez Gene ID	25776
Full Name	chibby family member 1, beta catenin antagonist
Synonyms	C22orf2,CBY,HS508I15A,PGEA1,PIGEA-14,PIGEA14,arb1
General protein information	Preferred Names chibby family member 1, beta catenin antagonist Names protein chibby homolog 1 ARPP-binding protein PKD2 interactor, Golgi and endoplasmic reticulum-associated 1 chibby CTNNB1-mediated transcription inhibitor chibby homolog 1 coiled-coil protein PIGEA-14 cytosolic leucine-rich protein polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein, 14 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	Beta-catenin is a transcriptional activator and oncoprotein involved in the development of several cancers. The protein encoded by this gene interacts directly with the C-terminal region of beta-catenin, inhibiting oncogenic beta-catenin-mediated transcriptional activation by competing with transcription factors for binding to beta-catenin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	607757

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002880.1	NP_001002880.2	protein chibby homolog 1 isoform b
NM_015373.3	NP_056188.1	protein chibby homolog 1 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CBY1	XP_003317289.1
Rattus norvegicus (Norway rat)	Cby1	NP_663709.1
Danio rerio (zebrafish)	cby1	NP_001003838.1
Homo sapiens (human)	CBY1	NP_001002880.2
Canis lupus familiaris (dog)	CBY1	XP_538373.3
Bos taurus (cattle)	CBY1	NP_777164.1
Mus musculus (house mouse)	Cby1	NP_082910.1
Gallus gallus (chicken)	CBY1	NP_001265078.1
Xenopus tropicalis (tropical clawed frog)	cby1	NP_001016253.1

Gene Ontology
Bibliography

Related articles in PubMed

Chibby 1: a new component of ?-catenin-signaling in chronic myeloid leukemia.
Mancini M, Soverini S, Gugliotta G, Santucci MA, Rosti G, Cavo M, Martinelli G, Castagnetti F
Oncotarget8(50)88244-88250(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Chibby1 knockdown promotes mesenchymal-to-epithelial transition-like changes.
Fischer V, Wong M, Li FQ, Takemaru KI
Cell cycle (Georgetown, Tex.)16(5)448-456(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

chronic Cby1 knockdown in colon cancer cells may counteract tumor progression by promoting the mesenchymal-to-epithelial transition process.
Title: Chibby1 knockdown promotes mesenchymal-to-epithelial transition-like changes.

FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane-remodeling processes.
Title: BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To Facilitate Ciliogenesis.

Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions.
Title: Conformational characterization of the intrinsically disordered protein Chibby: Interplay between structural elements in target recognition.

Results show the hypermethylation of CBY1 and BCL2-like11 promoters proceeding from DNMT1 is a crucial component of their reduced expression, but it is not directly involved in chronic myeloid leukemia resistance to imatinib.
Title: DNA methyltransferase 1 drives transcriptional down-modulation of ? catenin antagonist Chibby1 associated with the BCR-ABL1 gene of chronic myeloid leukemia.

CBY1 downregulation in CML comes from reduced protein stability when bound to 14-3-3sigma adapter protein. Dissociation raises CBY1 protein levels by enhancing its stability. The ubiquitin proteasome system reduces 14-3-3sigma-bound CBY1 stability by SUMOylation.
Title: 14-3-3 Binding and Sumoylation Concur to the Down-Modulation of ?-catenin Antagonist chibby 1 in Chronic Myeloid Leukemia.

The following CBY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CBY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu55567	NM_015373.3 Latest version!	Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03425	NM_001002880.1 Latest version!	Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CBY1 ( NM_015373.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056188.1 Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55567
Clone ID Related Accession (Same CDS sequence)	NM_015373.3
Accession Version	NM_015373.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	protein chibby homolog 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD702742.1, AL136686.1 and BC016139.1. On Aug 4, 2004 this sequence version replaced NM_015373.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform (a), which is shorter at the N-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136686.1, SRR1163658.386781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTTTCT TTGGGAATAC GTTCAGTCCG AAGAAGACAC CTCCTCGGAA GTCGGCATCT 
CTCTCCAACC TGCATTCTTT GGATCGATCA ACCCGGGAGG TGGAGCTGGG CTTGGAATAC 
GGATCCCCGA CTATGAACCT GGCAGGGCAA AGCCTGAAGT TTGAAAATGG CCAGTGGATA 
GCAGAGACAG GGGTTAGTGG CGGTGTGGAC CGGAGGGAGG TTCAGCGCCT TCGCAGGCGG 
AACCAGCAGT TGGAGGAAGA GAACAATCTC TTGCGGCTGA AAGTGGACAT CTTATTAGAC 
ATGCTTTCAG AGTCCACTGC TGAATCCCAC TTAATGGAGA AGGAACTGGA TGAACTGAGG 
ATCAGCCGGA AGAGAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056188.1
CDS	137..517
Translation	MPFFGNTFSPKKTPPRKSASLSNLHSLDRSTREVELGLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK

Target ORF information:

RefSeq Version	NM_015373.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015373.3

ORF Insert Sequence:

ATGCCTTTCT TTGGGAATAC GTTCAGTCCG AAGAAGACAC CTCCTCGGAA GTCGGCATCT 
CTCTCCAACC TGCATTCTTT GGATCGATCA ACCCGGGAGG TGGAGCTGGG CTTGGAATAC 
GGATCCCCGA CTATGAACCT GGCAGGGCAA AGCCTGAAGT TTGAAAATGG CCAGTGGATA 
GCAGAGACAG GGGTTAGTGG CGGTGTGGAC CGGAGGGAGG TTCAGCGCCT TCGCAGGCGG 
AACCAGCAGT TGGAGGAAGA GAACAATCTC TTGCGGCTGA AAGTGGACAT CTTATTAGAC 
ATGCTTTCAG AGTCCACTGC TGAATCCCAC TTAATGGAGA AGGAACTGGA TGAACTGAGG 
ATCAGCCGGA AGAGAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CBY1 ( NM_001002880.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001002880.2 Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03425
Clone ID Related Accession (Same CDS sequence)	NM_001002880.1
Accession Version	NM_001002880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	protein chibby homolog 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD626926.1, AU099418.1 and BC016139.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. The resulting isoform (b) is longer at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.458098.1, BX341980.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATAAAT GTCAGAAACA TGACGTTGAG TGTGAGAAGC CAGATGCAAA CGAGGACTCA 
CTGTGCAATT CTGTGCATGT ACAGTGGCCA GGAGAAGGGA GCACTGGCTT TGCTTTCATC 
AGGCCAAAGA TGCCTTTCTT TGGGAATACG TTCAGTCCGA AGAAGACACC TCCTCGGAAG 
TCGGCATCTC TCTCCAACCT GCATTCTTTG GATCGATCAA CCCGGGAGGT GGAGCTGGGC 
TTGGAATACG GATCCCCGAC TATGAACCTG GCAGGGCAAA GCCTGAAGTT TGAAAATGGC 
CAGTGGATAG CAGAGACAGG GGTTAGTGGC GGTGTGGACC GGAGGGAGGT TCAGCGCCTT 
CGCAGGCGGA ACCAGCAGTT GGAGGAAGAG AACAATCTCT TGCGGCTGAA AGTGGACATC 
TTATTAGACA TGCTTTCAGA GTCCACTGCT GAATCCCACT TAATGGAGAA GGAACTGGAT 
GAACTGAGGA TCAGCCGGAA GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002880.2
CDS	148..657
Translation	MDKCQKHDVECEKPDANEDSLCNSVHVQWPGEGSTGFAFIRPKMPFFGNTFSPKKTPPRKSASLSNLHSLDRSTREVELGLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK

Target ORF information:

RefSeq Version	NM_001002880.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chibby family member 1, beta catenin antagonist (CBY1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002880.1

ORF Insert Sequence:

ATGGATAAAT GTCAGAAACA TGACGTTGAG TGTGAGAAGC CAGATGCAAA CGAGGACTCA 
CTGTGCAATT CTGTGCATGT ACAGTGGCCA GGAGAAGGGA GCACTGGCTT TGCTTTCATC 
AGGCCAAAGA TGCCTTTCTT TGGGAATACG TTCAGTCCGA AGAAGACACC TCCTCGGAAG 
TCGGCATCTC TCTCCAACCT GCATTCTTTG GATCGATCAA CCCGGGAGGT GGAGCTGGGC 
TTGGAATACG GATCCCCGAC TATGAACCTG GCAGGGCAAA GCCTGAAGTT TGAAAATGGC 
CAGTGGATAG CAGAGACAGG GGTTAGTGGC GGTGTGGACC GGAGGGAGGT TCAGCGCCTT 
CGCAGGCGGA ACCAGCAGTT GGAGGAAGAG AACAATCTCT TGCGGCTGAA AGTGGACATC 
TTATTAGACA TGCTTTCAGA GTCCACTGCT GAATCCCACT TAATGGAGAA GGAACTGGAT 
GAACTGAGGA TCAGCCGGAA GAGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chibby 1: a new component of ?-catenin-signaling in chronic myeloid leukemia.
Oncotarget8(50)88244-88250(2017 Oct)
Mancini M,Soverini S,Gugliotta G,Santucci MA,Rosti G,Cavo M,Martinelli G,Castagnetti F

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Chibby1 knockdown promotes mesenchymal-to-epithelial transition-like changes.
Cell cycle (Georgetown, Tex.)16(5)448-456(2017 Mar)
Fischer V,Wong M,Li FQ,Takemaru KI

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CBY1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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