HYPK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HYPK
Entrez Gene ID	25764
Full Name	huntingtin interacting protein K
Synonyms	C15orf63,HSPC136
General protein information	Preferred Names huntingtin interacting protein K Names huntingtin-interacting protein K huntingtin yeast partner K
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.3
Disorder MIM:	612784

mRNA and Protein(s)

mRNA	Protein	Name
NM_016400.4	NP_057484.4	huntingtin-interacting protein K isoform 1
NM_001199885.1	NP_001186814.1	huntingtin-interacting protein K isoform 2
NM_016400.3	NP_057484.3	huntingtin-interacting protein K isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HYPK	NP_057484.3
	HYPK	XP_002804793.1
Bos taurus (cattle)	C21H15orf63	NP_001193315.1
Gallus gallus (chicken)	HYPK	NP_001264583.1
Canis lupus familiaris (dog)	HYPK	XP_544652.3
Mus musculus (house mouse)	Hypk	NP_080594.2
Xenopus tropicalis (tropical clawed frog)	hypk	NP_001120475.1
Pan troglodytes (chimpanzee)	LOC745332	XP_003951231.1
Rattus norvegicus (Norway rat)	Hypk	XP_006234935.1
Danio rerio (zebrafish)	hypk	NP_001120942.1
Drosophila melanogaster (fruit fly)	CG9922	NP_001262556.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004606	XP_315218.4

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis of HypK regulating N-terminal acetylation by the NatA complex.
Weyer FA, Gumiero A, Lapouge K, Bange G, Kopp J, Sinning I
Nature communications815726(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that HYPK, possibly with its interacting partners, induces autophagy. results further confirm that HYPK could also be involved in clearing mutant HTT aggregates by augmenting autophagy pathway.
Title: Chaperone-like protein HYPK and its interacting partners augment autophagy.

HYPK can act as negative regulator of heat shock response by repressing transcriptional activity of HSF1.
Title: Huntingtin interacting protein HYPK is a negative regulator of heat shock response and is downregulated in models of Huntington's Disease.

specific interaction of HYPK with HTT-N17 is crucial for the chaperone activity of HYPK
Title: Chaperone protein HYPK interacts with the first 17 amino acid region of Huntingtin and modulates mutant HTT-mediated aggregation and cytotoxicity.

By virtue of its flexibility and nascent peptide binding activity, HYPK may play an important role in assisting protein (re)folding.
Title: Conserved C-terminal nascent peptide binding domain of HYPK facilitates its chaperone-like activity.

Chaperone-like protein HYPK is induced by cellular stress and is under transcriptional regulation by HSF1.
Title: Transcription regulation of HYPK by Heat Shock Factor 1.

The following HYPK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HYPK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14803	NM_016400.3 Latest version!	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14390	NM_001199885.1 Latest version!	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu108894	NM_016400.4 Latest version!	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HYPK ( NM_016400.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057484.3 Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14803
Clone ID Related Accession (Same CDS sequence)	NM_016400.3
Accession Version	NM_016400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	huntingtin-interacting protein K isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ772100.1, BC019262.1 and AC018512.6. On Dec 24, 2010 this sequence version replaced NM_016400.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC019262.1, SRR1803612.420894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCGGCGGC GTGGTGAAAT AGATATGGCG ACCGAGGGGG ATGTGGAGCT GGAGTTGGAG 
ACTGAGACCA GTGGACCAGA GCGGCCTCCG GAGAAGCCAC GGAAACATGA CAGCGGTGCG 
GCGGACTTGG AGCGGGTCAC CGACTATGCA GAGGAGAAGG AGATCCAGAG TTCCAATCTG 
GAGACGGCCA TGTCTGTGAT TGGAGACAGA AGGTCCCGGG AGCAGAAAGC CAAACAGGAG 
CGGGAGAAAG AACTGGCAAA AGTCACTATC AAGAAGGAAG ATCTGGAGCT AATAATGACT 
GAGATGGAGA TATCTCGAGC AGCAGCAGAA CGCAGTTTGC GGGAACACAT GGGCAACGTG 
GTAGAGGCGC TTATTGCCCT AACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057484.3
CDS	179..568
Translation	MRRRGEIDMATEGDVELELETETSGPERPPEKPRKHDSGAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEALIALTN

Target ORF information:

RefSeq Version	NM_016400.3
Organism	Homo sapiens(human)
Definition	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016400.3

ORF Insert Sequence:

ATGCGGCGGC GTGGTGAAAT AGATATGGCG ACCGAGGGGG ATGTGGAGCT GGAGTTGGAG 
ACTGAGACCA GTGGACCAGA GCGGCCTCCG GAGAAGCCAC GGAAACATGA CAGCGGTGCG 
GCGGACTTGG AGCGGGTCAC CGACTATGCA GAGGAGAAGG AGATCCAGAG TTCCAATCTG 
GAGACGGCCA TGTCTGTGAT TGGAGACAGA AGGTCCCGGG AGCAGAAAGC CAAACAGGAG 
CGGGAGAAAG AACTGGCAAA AGTCACTATC AAGAAGGAAG ATCTGGAGCT AATAATGACT 
GAGATGGAGA TATCTCGAGC AGCAGCAGAA CGCAGTTTGC GGGAACACAT GGGCAACGTG 
GTAGAGGCGC TTATTGCCCT AACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HYPK ( NM_001199885.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186814.1 Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14390
Clone ID Related Accession (Same CDS sequence)	NM_001199885.1
Accession Version	NM_001199885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	huntingtin-interacting protein K isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ772100.1, BQ478474.1, BC019262.1 and AC018512.6. Transcript Variant: This variant (2) lacks an alternate exon resulting in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (2) has a distinct and shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ478474.1, AW168528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCGGC GTGGTGAAAT AGATATGGCG ACCGAGGGGG ATGTGGAGCT GGAGTTGGAG 
ACTGAGACCA GTGGACCAGA GCGGCCTCCG GAGAAGCCAC GGAAACATGA CAGCGGTGCG 
GCGGACTTGG AGCGGGTCAC CGACTATGCA GAGGAGAAGG AGATCCAGAG TTCCAATCTG 
GAGACGGGAG AAAGAACTGG CAAAAGTCAC TATCAAGAAG GAAGATCTGG AGCTAATAAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186814.1
CDS	179..424
Translation	MRRRGEIDMATEGDVELELETETSGPERPPEKPRKHDSGAADLERVTDYAEEKEIQSSNLETGERTGKSHYQEGRSGANND

Target ORF information:

RefSeq Version	NM_001199885.1
Organism	Homo sapiens(human)
Definition	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199885.1

ORF Insert Sequence:

ATGCGGCGGC GTGGTGAAAT AGATATGGCG ACCGAGGGGG ATGTGGAGCT GGAGTTGGAG 
ACTGAGACCA GTGGACCAGA GCGGCCTCCG GAGAAGCCAC GGAAACATGA CAGCGGTGCG 
GCGGACTTGG AGCGGGTCAC CGACTATGCA GAGGAGAAGG AGATCCAGAG TTCCAATCTG 
GAGACGGGAG AAAGAACTGG CAAAAGTCAC TATCAAGAAG GAAGATCTGG AGCTAATAAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HYPK ( NM_016400.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057484.4 Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108894
Clone ID Related Accession (Same CDS sequence)	NM_016400.4
Accession Version	NM_016400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	huntingtin-interacting protein K isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018512.6. On Aug 21, 2019 this sequence version replaced NM_016400.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.566946.1, DRR138528.1050900.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGCGACCG AGGGGGATGT GGAGCTGGAG TTGGAGACTG AGACCAGTGG ACCAGAGCGG 
CCTCCGGAGA AGCCACGGAA ACATGACAGC GGTGCGGCGG ACTTGGAGCG GGTCACCGAC 
TATGCAGAGG AGAAGGAGAT CCAGAGTTCC AATCTGGAGA CGGCCATGTC TGTGATTGGA 
GACAGAAGGT CCCGGGAGCA GAAAGCCAAA CAGGAGCGGG AGAAAGAACT GGCAAAAGTC 
ACTATCAAGA AGGAAGATCT GGAGCTAATA ATGACTGAGA TGGAGATATC TCGAGCAGCA 
GCAGAACGCA GTTTGCGGGA ACACATGGGC AACGTGGTAG AGGCGCTTAT TGCCCTAACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057484.4
CDS	25..390
Translation	MATEGDVELELETETSGPERPPEKPRKHDSGAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEALIALTN

Target ORF information:

RefSeq Version	NM_016400.4
Organism	Homo sapiens(human)
Definition	Homo sapiens huntingtin interacting protein K (HYPK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016400.4

ORF Insert Sequence:

ATGGCGACCG AGGGGGATGT GGAGCTGGAG TTGGAGACTG AGACCAGTGG ACCAGAGCGG 
CCTCCGGAGA AGCCACGGAA ACATGACAGC GGTGCGGCGG ACTTGGAGCG GGTCACCGAC 
TATGCAGAGG AGAAGGAGAT CCAGAGTTCC AATCTGGAGA CGGCCATGTC TGTGATTGGA 
GACAGAAGGT CCCGGGAGCA GAAAGCCAAA CAGGAGCGGG AGAAAGAACT GGCAAAAGTC 
ACTATCAAGA AGGAAGATCT GGAGCTAATA ATGACTGAGA TGGAGATATC TCGAGCAGCA 
GCAGAACGCA GTTTGCGGGA ACACATGGGC AACGTGGTAG AGGCGCTTAT TGCCCTAACC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis of HypK regulating N-terminal acetylation by the NatA complex.
Nature communications815726(2017 Jun)
Weyer FA,Gumiero A,Lapouge K,Bange G,Kopp J,Sinning I

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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HYPK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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