GAD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAD2
Entrez Gene ID	2572
Full Name	glutamate decarboxylase 2
Synonyms	GAD65
General protein information	Preferred Names glutamate decarboxylase 2 Names glutamate decarboxylase 2 65 kDa glutamic acid decarboxylase GAD-65 Glutamate decarboxylase-2 (pancreas) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) glutamate decarboxylase 65 kDa isoform
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p12.1
Summary	This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008].
Disorder MIM:	138275

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134366.1	NP_001127838.1	glutamate decarboxylase 2
NM_001134366.2	NP_001127838.1	glutamate decarboxylase 2
NM_000818.2	NP_000809.1	glutamate decarboxylase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAD2	NP_000809.1
Pan troglodytes (chimpanzee)	GAD2	XP_001157825.1
	GAD2	XP_002805636.1
Bos taurus (cattle)	GAD2	NP_001192652.1
Gallus gallus (chicken)	GAD2	XP_418596.3
Xenopus tropicalis (tropical clawed frog)	gad2	XP_002933179.1
Canis lupus familiaris (dog)	GAD2	NP_001070907.1
Mus musculus (house mouse)	Gad2	NP_032104.2
Rattus norvegicus (Norway rat)	Gad2	NP_036695.1
Danio rerio (zebrafish)	gad2	NP_001017708.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers.
Perri V, Gianchecchi E, Cifaldi L, Pellegrino M, Giorda E, Andreani M, Cappa M, Fierabracci A
PloS one12(12)e0189615(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

healthy donor NK cells showed similar degranulation against both GAD65 AA 114-122 pulsed and unpulsed APCs. The pathogenetic significance of the CD3-CD8dullCD56+ 'memory-like NK cell subset' with increased response upon secondary challenge in diabetics remains to be elucidated
Title: Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers.

Genetic variability in GAD2 and GAD1 contributes to risk of methamphetamine dependence in the Thai population.
Title: Association of polymorphisms in GAD1 and GAD2 genes with methamphetamine dependence.

Study propose that aberrant accumulation of immunogenic GAD65 in Golgi membranes facilitates inappropriate presentation to the immune system after release from stressed and/or damaged beta-cells, triggering autoimmunity.
Title: Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity.

UV exposed hydrogen peroxide produces hydroxyl radical that may cause protein damage (GAD-65) to the extent of generating neo-epitopes on the molecule, making it immunogenic.
Title: Immuno-chemistry of hydroxyl radical modified GAD-65: A possible role in experimental and human diabetes mellitus.

we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region
Title: Relationship between serum GAD-Ab and the genetic polymorphisms of GAD2 and type 2 diabetes mellitus.

The following GAD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17500	NM_001134366.2 Latest version!	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17500	NM_000818.2 Latest version!	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu17500	NM_001134366.1	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAD2 ( NM_001134366.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127838.1 Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.

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CloneID	OHu17500
Clone ID Related Accession (Same CDS sequence)	NM_001134366.1 , NM_001134366.2 , NM_000818.2
Accession Version	NM_001134366.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	glutamate decarboxylase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI438818.1 and M81882.1. Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.156428.1, SRR1660807.148035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127838.1
CDS	504..2261
Translation	MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_001134366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134366.1

ORF Insert Sequence:

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAD2 ( NM_001134366.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127838.1 Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17500
Clone ID Related Accession (Same CDS sequence)	NM_001134366.1 , NM_001134366.2 , NM_000818.2
Accession Version	NM_001134366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	glutamate decarboxylase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M81882.1 and AL162503.12. On Nov 22, 2018 this sequence version replaced NM_001134366.1. Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.175498.1, SRR1803616.132240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376261.8/ ENSP00000365437.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127838.1
CDS	37..1794
Translation	MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_001134366.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134366.2

ORF Insert Sequence:

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAD2 ( NM_000818.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000809.1 Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17500
Clone ID Related Accession (Same CDS sequence)	NM_001134366.1 , NM_001134366.2 , NM_000818.2
Accession Version	NM_000818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	glutamate decarboxylase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI438818.1, M74826.1 and BP392604.1. On Aug 26, 2008 this sequence version replaced NM_000818.1. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M74826.1, SRR1803611.101627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000809.1
CDS	504..2261
Translation	MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_000818.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000818.2

ORF Insert Sequence:

ATGGCATCTC CGGGCTCTGG CTTTTGGTCT TTCGGGTCGG AAGATGGCTC TGGGGATTCC 
GAGAATCCCG GCACAGCGCG AGCCTGGTGC CAAGTGGCTC AGAAGTTCAC GGGCGGCATC 
GGAAACAAAC TGTGCGCCCT GCTCTACGGA GACGCCGAGA AGCCGGCGGA GAGCGGCGGG 
AGCCAACCCC CGCGGGCCGC CGCCCGGAAG GCCGCCTGCG CCTGCGACCA GAAGCCCTGC 
AGCTGCTCCA AAGTGGATGT CAACTACGCG TTTCTCCATG CAACAGACCT GCTGCCGGCG 
TGTGATGGAG AAAGGCCCAC TTTGGCGTTT CTGCAAGATG TTATGAACAT TTTACTTCAG 
TATGTGGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA TCCTAATGAG 
CTTCTCCAAG AATATAATTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACAACTCT AAAATATGCA ATTAAAACAG GGCATCCTAG ATACTTCAAT 
CAACTTTCTA CTGGTTTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AATACTAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTTTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCAGGGGGCT CTGGCGATGG GATATTTTCT 
CCCGGTGGCG CCATATCTAA CATGTATGCC ATGATGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCTCTT CCCAGGCTCA TTGCCTTCAC GTCTGAACAT 
AGTCATTTTT CTCTCAAGAA GGGAGCTGCA GCCTTAGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATTCCATCTG ATCTTGAAAG AAGGATTCTT 
GAAGCCAAAC AGAAAGGGTT TGTTCCTTTC CTCGTGAGTG CCACAGCTGG AACCACCGTG 
TACGGAGCAT TTGACCCCCT CTTAGCTGTC GCTGACATTT GCAAAAAGTA TAAGATCTGG 
ATGCATGTGG ATGCAGCTTG GGGTGGGGGA TTACTGATGT CCCGAAAACA CAAGTGGAAA 
CTGAGTGGCG TGGAGAGGGC CAACTCTGTG ACGTGGAATC CACACAAGAT GATGGGAGTC 
CCTTTGCAGT GCTCTGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAA TTGCAACCAA 
ATGCATGCCT CCTACCTCTT TCAGCAAGAT AAACATTATG ACCTGTCCTA TGACACTGGA 
GACAAGGCCT TACAGTGCGG ACGCCACGTT GATGTTTTTA AACTATGGCT GATGTGGAGG 
GCAAAGGGGA CTACCGGGTT TGAAGCGCAT GTTGATAAAT GTTTGGAGTT GGCAGAGTAT 
TTATACAACA TCATAAAAAA CCGAGAAGGA TATGAGATGG TGTTTGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACATTCCT CCAAGCTTGC GTACTCTGGA AGACAATGAA 
GAGAGAATGA GTCGCCTCTC GAAGGTGGCT CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGAACCACAA TGGTCAGCTA CCAACCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTC 
ATCTCAAACC CAGCGGCAAC TCACCAAGAC ATTGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of GAD65 AA 114-122 reactive 'memory-like' NK cells in newly diagnosed Type 1 diabetic patients by HLA-class I pentamers.
PloS one12(12)e0189615(2017)
Perri V,Gianchecchi E,Cifaldi L,Pellegrino M,Giorda E,Andreani M,Cappa M,Fierabracci A

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GAD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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