NEGR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEGR1
Entrez Gene ID	257194
Full Name	neuronal growth regulator 1
Synonyms	DMML2433,IGLON4,KILON,Ntra
General protein information	Preferred Names neuronal growth regulator 1 Names neuronal growth regulator 1 IgLON family member 4 a kindred of IgLON neurotractin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.1
Disorder MIM:	613173

mRNA and Protein(s)

mRNA	Protein	Name
XM_017000961.1	XP_016856450.1	neuronal growth regulator 1 isoform X3
NM_173808.2	NP_776169.2	neuronal growth regulator 1 precursor
XM_011541201.2	XP_011539503.1	neuronal growth regulator 1 isoform X2
XM_011541200.2	XP_011539502.1	neuronal growth regulator 1 isoform X1
XM_011541201.3	XP_011539503.1	neuronal growth regulator 1 isoform X2
NM_173808.3	NP_776169.2	neuronal growth regulator 1 precursor
XM_011541200.3	XP_011539502.1	neuronal growth regulator 1 isoform X1
XM_017000961.2	XP_016856450.1	neuronal growth regulator 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NEGR1	XP_513488.2
Xenopus tropicalis (tropical clawed frog)	negr1	NP_001106537.1
	NEGR1	XP_001099596.1
Bos taurus (cattle)	NEGR1	NP_001192343.1
Gallus gallus (chicken)	NEGR1	NP_990187.1
Drosophila melanogaster (fruit fly)	Lac	NP_523713.2
Homo sapiens (human)	NEGR1	NP_776169.2
Canis lupus familiaris (dog)	NEGR1	XP_855525.2
Mus musculus (house mouse)	Negr1	NP_001034183.1
Rattus norvegicus (Norway rat)	Negr1	NP_067714.1
Danio rerio (zebrafish)	negr1	NP_001003851.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007500	XP_308376.4

Gene Ontology
Bibliography

Related articles in PubMed

Psychiatric Genomics: An Update and an Agenda.
Sullivan PF, Agrawal A, Bulik CM, Andreassen OA, B?rglum AD, Breen G, Cichon S, Edenberg HJ, Faraone SV, Gelernter J, Mathews CA, Nievergelt CM, Smoller JW, O'Donovan MC,
The American journal of psychiatry175(1)15-27(2018 Jan)

Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.
Zandon? MR, Sangalli CN, Campagnolo PD, Vitolo MR, Almeida S, Mattevi VS
Pediatric obesity12(1)85-92(2017 Feb)

Copy Number Variations in Candidate Genes and Intergenic Regions Affect Body Mass Index and Abdominal Obesity in Mexican Children.
Ant?nez-Ortiz DL, Flores-Alfaro E, Burguete-Garc?a AI, Bonnefond A, Peralta-Romero J, Froguel P, Espinoza-Rojo M, Cruz M
BioMed research international20172432957(2017)

The new obesity-associated protein, neuronal growth regulator 1 (NEGR1), is implicated in Niemann-Pick disease Type C (NPC2)-mediated cholesterol trafficking.
Kim H, Chun Y, Che L, Kim J, Lee S, Lee S
Biochemical and biophysical research communications482(4)1367-1374(2017 Jan)

Identification of 15 genetic loci associated with risk of major depression in individuals of European descent.
Hyde CL, Nagle MW, Tian C, Chen X, Paciga SA, Wendland JR, Tung JY, Hinds DA, Perlis RH, Winslow AR
Nature genetics48(9)1031-6(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significant associations were identified at 3.5 years old for TMEM18 rs6548238, NEGR1 rs2815752, BDNF rs10767664 and rs6265 (1 year old and 3.5 years old) with anthropometric phenotypes
Title: Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.

NEGR1 interacts with NPC2 and increases its protein stability; it has a role in obesity
Title: The new obesity-associated protein, neuronal growth regulator 1 (NEGR1), is implicated in Niemann-Pick disease Type C (NPC2)-mediated cholesterol trafficking.

Single nucleotide polymorphism in NEGR1 gene is associated with major depressive disorder.
Title: Identification of 15 genetic loci associated with risk of major depression in individuals of European descent.

We observed novel selection signals in CDKAL1 and NEGR1, well-known diabetes and obesity susceptibility genes
Title: Novel genomic signals of recent selection in an Ethiopian population.

Genetic variants associated with BMI and WHR in adults influence growth patterns and general and abdominal fat development from early childhood onwards.
Title: Adult adiposity susceptibility loci, early growth and general and abdominal fatness in childhood: the Generation R Study.

The following NEGR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEGR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17235	NM_173808.3 Latest version!	Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu78455	XM_011541200.3 Latest version!	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu78456	XM_011541201.3 Latest version!	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu87204	XM_017000961.2 Latest version!	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu78456	XM_011541201.2	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu17235	NM_173808.2	Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu78455	XM_011541200.2	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu87204	XM_017000961.1	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEGR1 ( NM_173808.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_776169.2 Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.

Price & Availability↑

CloneID	OHu17235
Clone ID Related Accession (Same CDS sequence)	NM_173808.2 , NM_173808.3
Accession Version	NM_173808.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645724.10, AL356600.13, AL359821.11, AL354949.10 and AL627317.7. On May 19, 2005 this sequence version replaced NM_173808.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.55914.1, SRR1660809.22430.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC CTCCAAGTAC AGCCCAGTAT 
GGAATTACCG GGAGCGCTGA TGTTCTTTTC TCCTGCTGGT ACCTTGTGTT GACACTGTCC 
TCTTTCACCA GCATATTCTA CCTGAAGAAT GCCATTCTAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776169.2
CDS	101..1165
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITGSADVLFSCWYLVLTLSSFTSIFYLKNAILQ

Target ORF information:

RefSeq Version	NM_173808.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173808.2

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC CTCCAAGTAC AGCCCAGTAT 
GGAATTACCG GGAGCGCTGA TGTTCTTTTC TCCTGCTGGT ACCTTGTGTT GACACTGTCC 
TCTTTCACCA GCATATTCTA CCTGAAGAAT GCCATTCTAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( NM_173808.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_776169.2 Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.

Price & Availability↑

CloneID	OHu17235
Clone ID Related Accession (Same CDS sequence)	NM_173808.2 , NM_173808.3
Accession Version	NM_173808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645724.10, AL356600.13, AL359821.11, AL354949.10 and AL627317.7. On Nov 22, 2018 this sequence version replaced NM_173808.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.104298.1, SRR1803614.27095.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357731.10/ ENSP00000350364.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC CTCCAAGTAC AGCCCAGTAT 
GGAATTACCG GGAGCGCTGA TGTTCTTTTC TCCTGCTGGT ACCTTGTGTT GACACTGTCC 
TCTTTCACCA GCATATTCTA CCTGAAGAAT GCCATTCTAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776169.2
CDS	46..1110
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITGSADVLFSCWYLVLTLSSFTSIFYLKNAILQ

Target ORF information:

RefSeq Version	NM_173808.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173808.3

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC CTCCAAGTAC AGCCCAGTAT 
GGAATTACCG GGAGCGCTGA TGTTCTTTTC TCCTGCTGGT ACCTTGTGTT GACACTGTCC 
TCTTTCACCA GCATATTCTA CCTGAAGAAT GCCATTCTAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_011541200.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539502.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu78455
Clone ID Related Accession (Same CDS sequence)	XM_011541200.2 , XM_011541200.3
Accession Version	XM_011541200.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC ATAAAAAAGA AGTCAGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539502.1
CDS	357..1319
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNHKKEVSS

Target ORF information:

RefSeq Version	XM_011541200.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541200.2

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC ATAAAAAAGA AGTCAGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_011541200.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539502.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu78455
Clone ID Related Accession (Same CDS sequence)	XM_011541200.2 , XM_011541200.3
Accession Version	XM_011541200.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC ATAAAAAAGA AGTCAGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539502.1
CDS	8..970
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNHKKEVSS

Target ORF information:

RefSeq Version	XM_011541200.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541200.3

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTTTG CTCCTACTAT TCAGGAAATT AAATCTGGCA CCGTGACCCC CGGACGCAGT 
GGCCTGATAA GATGTGAAGG TGCAGGTGTG CCGCCTCCAG CCTTTGAATG GTACAAAGGA 
GAGAAGAAGC TCTTCAATGG CCAACAAGGA ATTATTATTC AAAATTTTAG CACAAGATCC 
ATTCTCACTG TTACCAACGT GACACAGGAG CACTTCGGCA ATTATACCTG TGTGGCTGCC 
AACAAGCTAG GCACAACCAA TGCGAGCCTG CCTCTTAACC ATAAAAAAGA AGTCAGTAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_011541201.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539503.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu78456
Clone ID Related Accession (Same CDS sequence)	XM_011541201.2 , XM_011541201.3
Accession Version	XM_011541201.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTATC AGGGAAAAGA CTTGAACATC AGCATCTCAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539503.1
CDS	357..1061
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNYQGKDLNISISY

Target ORF information:

RefSeq Version	XM_011541201.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541201.2

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTATC AGGGAAAAGA CTTGAACATC AGCATCTCAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_011541201.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539503.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu78456
Clone ID Related Accession (Same CDS sequence)	XM_011541201.2 , XM_011541201.3
Accession Version	XM_011541201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTATC AGGGAAAAGA CTTGAACATC AGCATCTCAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539503.1
CDS	8..712
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNYQGKDLNISISY

Target ORF information:

RefSeq Version	XM_011541201.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541201.3

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTATC AGGGAAAAGA CTTGAACATC AGCATCTCAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_017000961.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856450.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu87204
Clone ID Related Accession (Same CDS sequence)	XM_017000961.1 , XM_017000961.2
Accession Version	XM_017000961.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856450.1
CDS	357..1025
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVN

Target ORF information:

RefSeq Version	XM_017000961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000961.1

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEGR1 ( XM_017000961.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856450.1 Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu87204
Clone ID Related Accession (Same CDS sequence)	XM_017000961.1 , XM_017000961.2
Accession Version	XM_017000961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal growth regulator 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856450.1
CDS	8..676
Translation	MDMMLLVQGACCSNQWLAAVLLSLCCLLPSCLPAGQSVDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVN

Target ORF information:

RefSeq Version	XM_017000961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal growth regulator 1 (NEGR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000961.2

ORF Insert Sequence:

ATGGACATGA TGCTGTTGGT GCAGGGTGCT TGTTGCTCGA ACCAGTGGCT GGCGGCGGTG 
CTCCTCAGCC TGTGCTGCCT GCTACCCTCC TGCCTCCCGG CTGGACAGAG TGTGGACTTC 
CCCTGGGCGG CCGTGGACAA CATGATGGTC AGAAAAGGGG ACACGGCGGT GCTTAGGTGT 
TATTTGGAAG ATGGAGCTTC AAAGGGTGCC TGGCTGAACC GGTCAAGTAT TATTTTTGCG 
GGAGGTGATA AGTGGTCAGT GGATCCTCGA GTTTCAATTT CAACATTGAA TAAAAGGGAC 
TACAGCCTCC AGATACAGAA TGTAGATGTG ACAGATGATG GCCCATACAC GTGTTCTGTT 
CAGACTCAAC ATACACCCAG AACAATGCAG GTGCATCTAA CTGTGCAAGT TCCTCCTAAG 
ATATATGACA TCTCAAATGA TATGACCGTC AATGAAGGAA CCAACGTCAC TCTTACTTGT 
TTGGCCACTG GGAAACCAGA GCCTTCCATT TCTTGGCGAC ACATCTCCCC ATCAGCAAAA 
CCATTTGAAA ATGGACAATA TTTGGACATT TATGGAATTA CAAGGGACCA GGCTGGGGAA 
TATGAATGCA GTGCGGAAAA TGATGTGTCA TTCCCAGATG TGAGGAAAGT AAAAGTTGTT 
GTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Psychiatric Genomics: An Update and an Agenda.
The American journal of psychiatry175(1)15-27(2018 Jan)
Sullivan PF,Agrawal A,Bulik CM,Andreassen OA,B?rglum AD,Breen G,Cichon S,Edenberg HJ,Faraone SV,Gelernter J,Mathews CA,Nievergelt CM,Smoller JW,O'Donovan MC,

Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.
Pediatric obesity12(1)85-92(2017 Feb)
Zandon? MR,Sangalli CN,Campagnolo PD,Vitolo MR,Almeida S,Mattevi VS

Copy Number Variations in Candidate Genes and Intergenic Regions Affect Body Mass Index and Abdominal Obesity in Mexican Children.
BioMed research international20172432957(2017)
Ant?nez-Ortiz DL,Flores-Alfaro E,Burguete-Garc?a AI,Bonnefond A,Peralta-Romero J,Froguel P,Espinoza-Rojo M,Cruz M

The new obesity-associated protein, neuronal growth regulator 1 (NEGR1), is implicated in Niemann-Pick disease Type C (NPC2)-mediated cholesterol trafficking.
Biochemical and biophysical research communications482(4)1367-1374(2017 Jan)
Kim H,Chun Y,Che L,Kim J,Lee S,Lee S

Identification of 15 genetic loci associated with risk of major depression in individuals of European descent.
Nature genetics48(9)1031-6(2016 Sep)
Hyde CL,Nagle MW,Tian C,Chen X,Paciga SA,Wendland JR,Tung JY,Hinds DA,Perlis RH,Winslow AR

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NEGR1 cDNA ORF clone, Homo sapiens(human)

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