PTF1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTF1A
Entrez Gene ID	256297
Full Name	pancreas specific transcription factor, 1a
Synonyms	PACA,PAGEN2,PTF1-p48,bHLHa29
General protein information	Preferred Names pancreas specific transcription factor, 1a Names pancreas transcription factor 1 subunit alpha bHLH transcription factor p48 class A basic helix-loop-helix protein 29 class II bHLH protein PTF1A exocrine pancreas-specific transcription factor p48 p48 DNA-binding subunit of transcription factor PTF1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p12.2
Summary	This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008].
Disorder MIM:	607194
Disorder Html:	Pancreatic and cerebellar agenesis, 609069 (3); Pancreatic agenesis 2, 615935 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_178161.3	NP_835455.1	pancreas transcription factor 1 subunit alpha
NM_178161.2	NP_835455.1	pancreas transcription factor 1 subunit alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ptf1a	NP_997524.1
Bos taurus (cattle)	PTF1A	XP_002707767.1
Mus musculus (house mouse)	Ptf1a	NP_061279.2
Xenopus tropicalis (tropical clawed frog)	ptf1a	XP_002933181.2
Homo sapiens (human)	PTF1A	NP_835455.1
Rattus norvegicus (Norway rat)	Ptf1a	NP_446416.1
Pan troglodytes (chimpanzee)	PTF1A	XP_001146416.2
Canis lupus familiaris (dog)	PTF1A	XP_850386.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A.
Hoang CQ, Hale MA, Azevedo-Pouly AC, Els?sser HP, Deering TG, Willet SG, Pan FC, Magnuson MA, Wright CV, Swift GH, MacDonald RJ
Molecular and cellular biology36(24)3033-3047(2016 Dec)

Isolated Pancreatic Aplasia Due to a Hypomorphic PTF1A Mutation.
Houghton JA, Swift GH, Shaw-Smith C, Flanagan SE, de Franco E, Caswell R, Hussain K, Mohamed S, Abdulrasoul M, Hattersley AT, MacDonald RJ, Ellard S
Diabetes65(9)2810-5(2016 09)

Loss of Ptf1a Leads to a Widespread Cell-Fate Misspecification in the Brainstem, Affecting the Development of Somatosensory and Viscerosensory Nuclei.
Iskusnykh IY, Steshina EY, Chizhikov VV
The Journal of neuroscience : the official journal of the Society for Neuroscience36(9)2691-710(2016 Mar)

Variable Phenotype of Diabetes Mellitus in Siblings with a Homozygous PTF1A Enhancer Mutation.
Gonc EN, Ozon A, Alikasifoglu A, Halilo?lu M, Ellard S, Shaw-Smith C, Kandemir N
Hormone research in paediatrics84(3)206-11(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study describes the wide range of gene control by PTF1A that maintains the specific characteristics of pancreatic acinar cell identity and differentiation. PTF1A controls the pancreatic acinar transcription program by direct action at a thousand genes and in collaboration with other less cell type-restricted factors to ensure acinar cell homeostasis and to suppress other cell-type-specific programs.
Title: Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A.

Data show isolated Pancreatic Aplasia Due to a Hypomorphic PTF1A Mutation
Title: Isolated Pancreatic Aplasia Due to a Hypomorphic PTF1A Mutation.

The results of this study suggested Ptf1a-dependent cell-fate misspecification as a novel mechanism of human brainstem pathology.
Title: Loss of Ptf1a Leads to a Widespread Cell-Fate Misspecification in the Brainstem, Affecting the Development of Somatosensory and Viscerosensory Nuclei.

Loss of Ptf1a alone is sufficient to induce acinar-to-ductal metaplasia, potentiate inflammation, and induce a KRAS-permissive, pancreatic ductal adenocarcinoma-like gene expression profile.
Title: The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.

PTF1A enhancer mutations have been shown to cause neonatal diabetes associated with pancreatic agenesis
Title: Variable Phenotype of Diabetes Mellitus in Siblings with a Homozygous PTF1A Enhancer Mutation.

The following PTF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19480	NM_178161.3 Latest version!	Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu19480	NM_178161.2	Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTF1A ( NM_178161.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_835455.1 Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.

Price & Availability↑

CloneID	OHu19480
Clone ID Related Accession (Same CDS sequence)	NM_178161.3 , NM_178161.2
Accession Version	NM_178161.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	pancreas transcription factor 1 subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139281.7, BM055347.1 and CK904916.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_178161.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138526.192110.1, DRR138526.189802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376504.4/ ENSP00000365687.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT 
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG 
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC 
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG 
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC 
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG 
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC 
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC 
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG 
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG 
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC 
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT 
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC 
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC 
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835455.1
CDS	220..1206
Translation	MDAVLLEHFPGGLDAFPSSYFDEDDFFTDQSSRDPLEDGDELLADEQAEVEFLSHQLHEYCYRDGACLLLQPAPPAAPLALAPPSSGGLGEPDDGGGGGYCCETGAPPGGFPYSPGSPPSCLAYPCAGAAVLSPGARLRGLSGAAAAAARRRRRVRSEAELQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQADLPLRGGGAGGCGGPGGGGRLGGDSPGSQAQKVIICHRGTRSPSPSDPDYGLPPLAGHSLSWTDEKQLKEQNIIRTAKVWTPEDPRKLNSKSSFNNIENEPPFEFVS

Target ORF information:

RefSeq Version	NM_178161.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178161.3

ORF Insert Sequence:

ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT 
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG 
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC 
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG 
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC 
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG 
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC 
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC 
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG 
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG 
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC 
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT 
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC 
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC 
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTF1A ( NM_178161.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_835455.1 Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.

Price & Availability↑

CloneID	OHu19480
Clone ID Related Accession (Same CDS sequence)	NM_178161.3 , NM_178161.2
Accession Version	NM_178161.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-29
Organism	Homo sapiens(human)
Product	pancreas transcription factor 1 subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139281.7, BM055347.1 and CK904916.1. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2006 this sequence version replaced NM_178161.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK904917.1, BM055347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT 
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG 
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC 
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG 
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC 
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG 
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC 
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC 
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG 
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG 
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC 
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT 
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC 
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC 
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835455.1
CDS	1..987
Translation	MDAVLLEHFPGGLDAFPSSYFDEDDFFTDQSSRDPLEDGDELLADEQAEVEFLSHQLHEYCYRDGACLLLQPAPPAAPLALAPPSSGGLGEPDDGGGGGYCCETGAPPGGFPYSPGSPPSCLAYPCAGAAVLSPGARLRGLSGAAAAAARRRRRVRSEAELQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQADLPLRGGGAGGCGGPGGGGRLGGDSPGSQAQKVIICHRGTRSPSPSDPDYGLPPLAGHSLSWTDEKQLKEQNIIRTAKVWTPEDPRKLNSKSSFNNIENEPPFEFVS

Target ORF information:

RefSeq Version	NM_178161.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pancreas associated transcription factor 1a (PTF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178161.2

ORF Insert Sequence:

ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT 
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG 
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC 
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG 
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC 
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG 
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC 
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC 
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG 
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG 
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC 
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT 
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC 
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC 
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional Maintenance of Pancreatic Acinar Identity, Differentiation, and Homeostasis by PTF1A.
Molecular and cellular biology36(24)3033-3047(2016 Dec)
Hoang CQ,Hale MA,Azevedo-Pouly AC,Els?sser HP,Deering TG,Willet SG,Pan FC,Magnuson MA,Wright CV,Swift GH,MacDonald RJ

Isolated Pancreatic Aplasia Due to a Hypomorphic PTF1A Mutation.
Diabetes65(9)2810-5(2016 09)
Houghton JA,Swift GH,Shaw-Smith C,Flanagan SE,de Franco E,Caswell R,Hussain K,Mohamed S,Abdulrasoul M,Hattersley AT,MacDonald RJ,Ellard S

Loss of Ptf1a Leads to a Widespread Cell-Fate Misspecification in the Brainstem, Affecting the Development of Somatosensory and Viscerosensory Nuclei.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(9)2691-710(2016 Mar)
Iskusnykh IY,Steshina EY,Chizhikov VV

Variable Phenotype of Diabetes Mellitus in Siblings with a Homozygous PTF1A Enhancer Mutation.
Hormone research in paediatrics84(3)206-11(2015)
Gonc EN,Ozon A,Alikasifoglu A,Halilo?lu M,Ellard S,Shaw-Smith C,Kandemir N

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PTF1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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