COL6A5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A5
Entrez Gene ID	256076
Full Name	collagen type VI alpha 5 chain
Synonyms	COL29A1,VWA4
General protein information	Preferred Names collagen type VI alpha 5 chain Names collagen alpha-5(VI) chain collagen, type VI, alpha 5 collagen, type XXIX, alpha 1 von Willebrand factor A domain-containing protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.1
Summary	This gene encodes a member of the collagen superfamily of proteins. The encoded protein contains multiple von Willebrand factor A-like domains and may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Polymorphisms in this gene may be linked to dermal phenotypes, such as eczema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013].
Disorder MIM:	611916

mRNA and Protein(s)

mRNA	Protein	Name
NM_153264.6	NP_694996.5	collagen alpha-5(VI) chain isoform 2 precursor
NM_001278298.1	NP_001265227.1	collagen alpha-5(VI) chain isoform 1 precursor
XM_011512621.2	XP_011510923.1	collagen alpha-5(VI) chain isoform X1
XM_011512623.2	XP_011510925.1	collagen alpha-5(VI) chain isoform X3
XM_011512622.2	XP_011510924.1	collagen alpha-5(VI) chain isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Col6a5	NP_001161395.1
Canis lupus familiaris (dog)	COL6A5	XP_853265.3
Rattus norvegicus (Norway rat)	Col6a5	XP_006243950.1
Homo sapiens (human)	COL6A5	NP_001265227.1
Xenopus tropicalis (tropical clawed frog)	col6a6	XP_002937788.2
Pan troglodytes (chimpanzee)	COL6A5	XP_526306.3
	LOC719331	XP_002808380.1

Gene Ontology
Bibliography

Related articles in PubMed

Longitudinal exome-wide association study to identify genetic susceptibility loci for hypertension in a Japanese population.
Yasukochi Y, Sakuma J, Takeuchi I, Kato K, Oguri M, Fujimaki T, Horibe H, Yamada Y
Experimental & molecular medicine49(12)e409;(2017 Dec)

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Glavey SV, Naba A, Manier S, Clauser K, Tahri S, Park J, Reagan MR, Moschetta M, Mishima Y, Gambella M, Rocci A, Sacco A, O'Dwyer ME, Asara JM, Palumbo A, Roccaro AM, Hynes RO, Ghobrial IM
Leukemia31(11)2426-2434(2017 Nov)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

COL6A5 variants in familial neuropathic chronic itch.
Martinelli-Boneschi F, Colombi M, Castori M, Devigili G, Eleopra R, Malik RA, Ritelli M, Zoppi N, Dordoni C, Sorosina M, Grammatico P, Fadavi H, Gerrits MM, Almomani R, Faber CG, Merkies IS, Toniolo D, , Cocca M, Doglioni C, Waxman SG, Dib-Hajj SD, Taiana MM, Sassone J, Lombardi R, Cazzato D, Zauli A, Santoro S, Marchi M, Lauria G
Brain : a journal of neurology140(3)555-567(2017 Mar)

EMR-linked GWAS study: investigation of variation landscape of loci for body mass index in children.
Namjou B, Keddache M, Marsolo K, Wagner M, Lingren T, Cobb B, Perry C, Kennebeck S, Holm IA, Li R, Crimmins NA, Martin L, Solti I, Kohane IS, Harley JB
Frontiers in genetics4268(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that an association between COL6A5 gene and familiar chronic itch, suggesting a new contributor to the pathogenesis of neuropathic itch.
Title: COL6A5 variants in familial neuropathic chronic itch.

The collagen VI alpha5 chain is selectively localized at the basement membrane of myotendinous junctions.
Title: Expression of collagen VI ?5 and ?6 chains in human muscle and in Duchenne muscular dystrophy-related muscle fibrosis.

Our results suggest that COL29A1 is unlikely to contain genetic variants that have a major effect on eczema or atopy susceptibility.
Title: A comprehensive analysis of the COL29A1 gene does not support a role in eczema.

localization of alpha5, and to a lesser extent alpha6, is restricted to the papillary dermis, where the protein mainly colocalizes with collagen fibrils; both chains were found around blood vessels
Title: Expression of the collagen VI ?5 and ?6 chains in normal human skin and in skin of patients with collagen VI-related myopathies.

Observational study of gene-disease association. (HuGE Navigator)
Title: Variation in the COL29A1 gene in German patients with atopic dermatitis, asthma and chronic obstructive pulmonary disease.

The following COL6A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04409	NM_001278298.1 Latest version!	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$929.50-$1301.30 $1859.00
OHu78357	XM_011512622.2 Latest version!	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu78358	XM_011512623.2 Latest version!	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu21032	NM_153264.6 Latest version!	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu78356	XM_011512621.2 Latest version!	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A5 ( NM_001278298.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265227.1 Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04409
Clone ID Related Accession (Same CDS sequence)	NM_001278298.1
Accession Version	NM_001278298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	collagen alpha-5(VI) chain isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU085556.1, AM906083.1 and AI743837.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGAA AAATGTCCAG CATATCCAAC AGAGCTAGTA 
TTTGCTCTGG ACAATTCCTA CGATGTCACA GAAGAGAGCT TTAATAAAAC ACGGGACATC 
ATCACTTCCA TTGTCAATGA CCTTAACATC AGGGAAAATA ACTGTCCTGT GGGAGCAAGA 
GTTGCCATGG TTTCCTATAA CTCAGGCACC AGCTATCTCA TCCGCTGGTC TGACTACAAT 
AGGAAGAAGC AGCTCCTCCA GCAGCTTTCC CAAATAAAAT ATCAAGACAC CACAGAGCCC 
CGAGATGTTG GTAATGCAAT GAGGTTTGTG ACCCGCAACG TGTTCAAGCG GACGTATGCA 
GGAGCCAACG TGAGGAGAGT TGCTGTGTTT TTTAGCAATG GTCAAACAGC CAGTAGGTCA 
TCCATCATCA CGGCCACCAT GGAGTTTAGT GCCCTGGATA TCAGTCCAAC AGTCTTTGCT 
TTTGATGAGA GAGTTTTCCT TGAAGCTTTT GGGTTTGACA ACACTGGAAC ATTTCAGGTG 
ATTCCAGTTC CTCCAAATGG GGAAAATCAA ACATTAGAAA GACTTCGGCG CTGTGCACTT 
TGCTATGATA AATGTTTTCC AAATGCTTGC ATTCGAGAGG CTTTCTTACC TGAAGATTCA 
TACATGGATG TAGTCTTCCT CATAGACAAT TCTCGGAATA TAGCAAAGGA TGAGTTTAAG 
GCTGTGAAAG CCTTGGTGAG CTCAGTGATT GACAACTTCA ACATTGCTTC AGACCCTTTA 
ATCTCAGACT CTGGTGATAG GATTGCTTTG TTGAGCTATT CTCCTTGGGA AAGTTCCAGG 
AGAAAGATGG GTACAGTAAA AACAGAGTTT GATTTCATCA CTTATGACAA CCAACTCCTA 
ATGAAGAATC ACATCCAGAC TTCCTTCCAA CAGCTAAATG GAGAAGCAAC AATTGGTCGT 
GCCCTACTGT GGACCACTGA AAATCTTTTT CCAGAAACAC CCTATCTAAG AAAACACAAG 
GTCATCTTTG TGGTCTCAGC TGGAGAAAAT TATGAGAGAA AAGAATTTGT AAAAATGATG 
GCTTTGAGGG CTAAGTGTCA AGGCTACGTC ATATTTGTGA TTTCTCTGGG CTCTACACGT 
AAGGATGACA TGGAGGAGTT AGCCAGCTAC CCACTTGATC AACACCTGAT ACAGCTTGGG 
AGAATACATA AACCAGATCT GAATTATATT GCGAAGTTCT TAAAGCCATT TTTATACTCG 
GTCAGGCGGG GATTCAATCA GTACCCACCA CCGATGCTTG AGGATGCCTG TAGACTCATC 
AATTTAGGAG GAGAGAATAT TCAAAATGAT GGTTTCCAAT TTGTTACTGA GCTACAAGAG 
GATTTTTTGG GAGGTAATGG CTTCATTGGC CAAGAATTAA ATTCTGGGAG AGAATCACCT 
TTTGTAAAGA CGGAAGACAA TGGAAGTGAC TATTTGGTTT ACCTTCCAAG CCAAATGTTT 
GAGCCACAAA AATTAATGAT CAATTATGAA AAAGATCAAA AATCTGCAGA AATTGCAAGT 
CTCACTTCTG GACATGAAAA TTATGGCAGA AAAGAAGAAC CAGATCATAC TTATGAACCT 
GGAGATGTCT CTCTTCAAGA ATATTACATG GATGTGGCTT TCCTCATAGA TGCTTCCCAA 
AGAGTAGGAA GTGATGAGTT TAAGGAAGTA AAAGCTTTTA TAACCTCAGT GCTTGATTAC 
TTTCACATTG CCCCCACTCC ACTGACCTCC ACCTTAGGAG ACAGGGTTGC TGTCCTGAGC 
TACTCTCCTC CAGGCTATAT GCCTAACACT GAAGAATGCC CTGTCTACCT GGAATTTGAT 
TTGGTTACTT ATAACAGTAT ACACCAAATG AAACATCATC TCCAAGACTC TCAACAGCTC 
AATGGAGATG TTTTTATTGG CCATGCCTTG CAGTGGACAA TTGACAATGT CTTTGTAGGA 
ACCCCCAATC TGAGGAAAAA CAAAGTTATC TTTGTAATAT CTGCTGGCGA AACCAACTCT 
TTAGACAAAG ACGTCTTAAG GAATGTGTCT CTGAGAGCCA AGTGTCAAGG CTACTCCATA 
TTTGTGTTTT CCTTTGGCCC TAAACACAAT GACAAAGAAT TAGAAGAATT AGCCAGCCAC 
CCTCTGGATC ATCACTTGGT CCAACTTGGC CGAACCCACA AGCCAGATTG GAACTATATC 
ATCAAGTTTG TCAAGCCATT TGTCCATTTA ATCAGACGTG CCATCAACAA ATATCCCACC 
GAAGATATGA AAGCCACATG TGTTAACATG ACCTCTCCCA ACCCAGAGAA CGGTGGCACA 
GAAAACACTG TATTACTTCT TCCTGGGATA TATGAAATAA AGACAGAAAA TGGTGATCTG 
TTTGATGAAT TTGATTCCCA GGCACAACAT CTCCTTGTAT TAGGGAACAA TCATAGTAGT 
GGTTCTGAGA CAGCTACTGA TTTGATGCAG AAGTTATATC TGCTCTTTTC AACTGAGAAA 
CTGGCAATGA AAGACAAGGA AAAAGCACAT TTAGAAGAAA TTTCAGCTCT AGTGGTTGAT 
AAACAGCAAG AAAAAGAAGA TGGTGAAGAT ACAAGGTCAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265227.1
CDS	495..8339
Translation	MKILLIIFVLIIWTETLADQSPGPGPVYADVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKDVTIFSVGVYNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLLDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDLGICVLVLGIGDVYKEHLLPITGNSEKIITFQDFDKLKNVDVKKRIIREICQSCGKTNCFMDIVVGFDISTHVQGQPLFQGHPQLESYLPGILEDISSIKGVSCGAGTEAQVSLAFKVNSDQGFPAKFQIYQKAVFDSLLQVNVSGPTHLNAQFLRSLWDTFKDKSASRGQVLLIFSDGLQSESNIMLENQSDRLREAGLDALLVVSLNTTAHHEFSSFEFGKRFDYRTHLTIGMRELGKKLSQYLGNIAERTCCCTFCKCPGIPGPHGTRGLQAMKGSQGLKGSRGHRGEDGNPGVRGDTGPQGDKGIAGCPGAWGQKGLKGFSGPKGGHGDDGIDGLDGEEGCHGFPGIKGEKGDPGSQGSPGSRGAPGQYGEKGFPGDPGNPGQNNNIKGQKGSKGEQGRQGRSGQKGVQGSPSSRGSRGREGQRGLRGVSGEPGNPGPTGTLGAEGLQGPQGSQGNPGRKGEKGSQGQKGPQGSPGLMGAKGSTGRPGLLGKKGEPGLPGDLGPVGQTGQRGRQGDSGIPGYGQMGRKGVKGPRGFPGDAGQKGDIGNPGIPGGPGPKGFRGLALTVGLKGEEGSRGLPGPPGQRGIKGMAGQPVYSQCDLIRFLREHSPCWKEKCPAYPTELVFALDNSYDVTEESFNKTRDIITSIVNDLNIRENNCPVGARVAMVSYNSGTSYLIRWSDYNRKKQLLQQLSQIKYQDTTEPRDVGNAMRFVTRNVFKRTYAGANVRRVAVFFSNGQTASRSSIITATMEFSALDISPTVFAFDERVFLEAFGFDNTGTFQVIPVPPNGENQTLERLRRCALCYDKCFPNACIREAFLPEDSYMDVVFLIDNSRNIAKDEFKAVKALVSSVIDNFNIASDPLISDSGDRIALLSYSPWESSRRKMGTVKTEFDFITYDNQLLMKNHIQTSFQQLNGEATIGRALLWTTENLFPETPYLRKHKVIFVVSAGENYERKEFVKMMALRAKCQGYVIFVISLGSTRKDDMEELASYPLDQHLIQLGRIHKPDLNYIAKFLKPFLYSVRRGFNQYPPPMLEDACRLINLGGENIQNDGFQFVTELQEDFLGGNGFIGQELNSGRESPFVKTEDNGSDYLVYLPSQMFEPQKLMINYEKDQKSAEIASLTSGHENYGRKEEPDHTYEPGDVSLQEYYMDVAFLIDASQRVGSDEFKEVKAFITSVLDYFHIAPTPLTSTLGDRVAVLSYSPPGYMPNTEECPVYLEFDLVTYNSIHQMKHHLQDSQQLNGDVFIGHALQWTIDNVFVGTPNLRKNKVIFVISAGETNSLDKDVLRNVSLRAKCQGYSIFVFSFGPKHNDKELEELASHPLDHHLVQLGRTHKPDWNYIIKFVKPFVHLIRRAINKYPTEDMKATCVNMTSPNPENGGTENTVLLLPGIYEIKTENGDLFDEFDSQAQHLLVLGNNHSSGSETATDLMQKLYLLFSTEKLAMKDKEKAHLEEISALVVDKQQEKEDGEDTRSS

Target ORF information:

RefSeq Version	NM_001278298.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278298.1

ORF Insert Sequence:

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGAA AAATGTCCAG CATATCCAAC AGAGCTAGTA 
TTTGCTCTGG ACAATTCCTA CGATGTCACA GAAGAGAGCT TTAATAAAAC ACGGGACATC 
ATCACTTCCA TTGTCAATGA CCTTAACATC AGGGAAAATA ACTGTCCTGT GGGAGCAAGA 
GTTGCCATGG TTTCCTATAA CTCAGGCACC AGCTATCTCA TCCGCTGGTC TGACTACAAT 
AGGAAGAAGC AGCTCCTCCA GCAGCTTTCC CAAATAAAAT ATCAAGACAC CACAGAGCCC 
CGAGATGTTG GTAATGCAAT GAGGTTTGTG ACCCGCAACG TGTTCAAGCG GACGTATGCA 
GGAGCCAACG TGAGGAGAGT TGCTGTGTTT TTTAGCAATG GTCAAACAGC CAGTAGGTCA 
TCCATCATCA CGGCCACCAT GGAGTTTAGT GCCCTGGATA TCAGTCCAAC AGTCTTTGCT 
TTTGATGAGA GAGTTTTCCT TGAAGCTTTT GGGTTTGACA ACACTGGAAC ATTTCAGGTG 
ATTCCAGTTC CTCCAAATGG GGAAAATCAA ACATTAGAAA GACTTCGGCG CTGTGCACTT 
TGCTATGATA AATGTTTTCC AAATGCTTGC ATTCGAGAGG CTTTCTTACC TGAAGATTCA 
TACATGGATG TAGTCTTCCT CATAGACAAT TCTCGGAATA TAGCAAAGGA TGAGTTTAAG 
GCTGTGAAAG CCTTGGTGAG CTCAGTGATT GACAACTTCA ACATTGCTTC AGACCCTTTA 
ATCTCAGACT CTGGTGATAG GATTGCTTTG TTGAGCTATT CTCCTTGGGA AAGTTCCAGG 
AGAAAGATGG GTACAGTAAA AACAGAGTTT GATTTCATCA CTTATGACAA CCAACTCCTA 
ATGAAGAATC ACATCCAGAC TTCCTTCCAA CAGCTAAATG GAGAAGCAAC AATTGGTCGT 
GCCCTACTGT GGACCACTGA AAATCTTTTT CCAGAAACAC CCTATCTAAG AAAACACAAG 
GTCATCTTTG TGGTCTCAGC TGGAGAAAAT TATGAGAGAA AAGAATTTGT AAAAATGATG 
GCTTTGAGGG CTAAGTGTCA AGGCTACGTC ATATTTGTGA TTTCTCTGGG CTCTACACGT 
AAGGATGACA TGGAGGAGTT AGCCAGCTAC CCACTTGATC AACACCTGAT ACAGCTTGGG 
AGAATACATA AACCAGATCT GAATTATATT GCGAAGTTCT TAAAGCCATT TTTATACTCG 
GTCAGGCGGG GATTCAATCA GTACCCACCA CCGATGCTTG AGGATGCCTG TAGACTCATC 
AATTTAGGAG GAGAGAATAT TCAAAATGAT GGTTTCCAAT TTGTTACTGA GCTACAAGAG 
GATTTTTTGG GAGGTAATGG CTTCATTGGC CAAGAATTAA ATTCTGGGAG AGAATCACCT 
TTTGTAAAGA CGGAAGACAA TGGAAGTGAC TATTTGGTTT ACCTTCCAAG CCAAATGTTT 
GAGCCACAAA AATTAATGAT CAATTATGAA AAAGATCAAA AATCTGCAGA AATTGCAAGT 
CTCACTTCTG GACATGAAAA TTATGGCAGA AAAGAAGAAC CAGATCATAC TTATGAACCT 
GGAGATGTCT CTCTTCAAGA ATATTACATG GATGTGGCTT TCCTCATAGA TGCTTCCCAA 
AGAGTAGGAA GTGATGAGTT TAAGGAAGTA AAAGCTTTTA TAACCTCAGT GCTTGATTAC 
TTTCACATTG CCCCCACTCC ACTGACCTCC ACCTTAGGAG ACAGGGTTGC TGTCCTGAGC 
TACTCTCCTC CAGGCTATAT GCCTAACACT GAAGAATGCC CTGTCTACCT GGAATTTGAT 
TTGGTTACTT ATAACAGTAT ACACCAAATG AAACATCATC TCCAAGACTC TCAACAGCTC 
AATGGAGATG TTTTTATTGG CCATGCCTTG CAGTGGACAA TTGACAATGT CTTTGTAGGA 
ACCCCCAATC TGAGGAAAAA CAAAGTTATC TTTGTAATAT CTGCTGGCGA AACCAACTCT 
TTAGACAAAG ACGTCTTAAG GAATGTGTCT CTGAGAGCCA AGTGTCAAGG CTACTCCATA 
TTTGTGTTTT CCTTTGGCCC TAAACACAAT GACAAAGAAT TAGAAGAATT AGCCAGCCAC 
CCTCTGGATC ATCACTTGGT CCAACTTGGC CGAACCCACA AGCCAGATTG GAACTATATC 
ATCAAGTTTG TCAAGCCATT TGTCCATTTA ATCAGACGTG CCATCAACAA ATATCCCACC 
GAAGATATGA AAGCCACATG TGTTAACATG ACCTCTCCCA ACCCAGAGAA CGGTGGCACA 
GAAAACACTG TATTACTTCT TCCTGGGATA TATGAAATAA AGACAGAAAA TGGTGATCTG 
TTTGATGAAT TTGATTCCCA GGCACAACAT CTCCTTGTAT TAGGGAACAA TCATAGTAGT 
GGTTCTGAGA CAGCTACTGA TTTGATGCAG AAGTTATATC TGCTCTTTTC AACTGAGAAA 
CTGGCAATGA AAGACAAGGA AAAAGCACAT TTAGAAGAAA TTTCAGCTCT AGTGGTTGAT 
AAACAGCAAG AAAAAGAAGA TGGTGAAGAT ACAAGGTCAT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A5 ( XM_011512622.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510924.1 Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X2, mRNA.

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CloneID	OHu78357
Clone ID Related Accession (Same CDS sequence)	XM_011512622.2
Accession Version	XM_011512622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAATTCTGG GGCTCTCATC TGGGGACACC CACATCTTCC TGGTCTTGTG CTTGGCCAAG 
CTAGTCTGGA CTGGAATTTG GCTTCTTTGT CTCAAGCTCT TGATCCTCAG CCTCTGCTTA 
AGAACTTGTT CAAAGTTATT TAAGGGCTCG CTCTTTCAGG ACATCCTCTC CATGGTTCTG 
ATTCTCTGTA AAATGTGGAC TGTCTACTCT GTGTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510924.1
CDS	369..5747
Translation	MKILLIIFVLIIWTETLADQSPGPGPVYADVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKDVTIFSVGVYNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLLDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDLGICVLVLGIGDVYKEHLLPITGNSEKIITFQDFDKLKNVDVKKRIIREICQSCGKTNCFMDIVVGFDISTHVQGQPLFQGHPQLESYLPGILEDISSIKGVSCGAGTEAQVSLAFKVNSDQGFPAKFQIYQKAVFDSLLQVNVSGPTHLNAQFLRSLWDTFKDKSASRGQVLLIFSDGLQSESNIMLENQSDRLREAGLDALLVVSLNTTAHHEFSSFEFGKRFDYRTHLTIGMRELGKKLSQYLGNIAERTCCCTFCKCPGIPGPHGTRGLQAMKGSQGLKGSRGHRGEDGNPGVRGDTGPQGDKGIAGCPGAWGQKGLKGFSGPKGGHGDDGIDGLDGEEGCHGFPGIKGEKGDPGSQGSPGSRGAPGQYGEKGFPGDPGNPGQNNNIKGQKGSKGEQGRQGRSGQKGVQGSPSSRGSRGREGQRGLRGVSGEPGNPGPTGTLGAEGLQGPQGSQGNPGRKGEKGSQGQKGPQGSPGLMGAKGSTGRPGLLGKKGEPGLPGDLGPVGQTGQRGRQGDSGIPGYGQMGRKGVKGPRGFPGDAGQKGDIGNPGIPGGPGPKGFRGLALTVGLKGEEGSRGLPGPPGQRILGLSSGDTHIFLVLCLAKLVWTGIWLLCLKLLILSLCLRTCSKLFKGSLFQDILSMVLILCKMWTVYSVY

Target ORF information:

RefSeq Version	XM_011512622.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512622.2

ORF Insert Sequence:

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAATTCTGG GGCTCTCATC TGGGGACACC CACATCTTCC TGGTCTTGTG CTTGGCCAAG 
CTAGTCTGGA CTGGAATTTG GCTTCTTTGT CTCAAGCTCT TGATCCTCAG CCTCTGCTTA 
AGAACTTGTT CAAAGTTATT TAAGGGCTCG CTCTTTCAGG ACATCCTCTC CATGGTTCTG 
ATTCTCTGTA AAATGTGGAC TGTCTACTCT GTGTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A5 ( XM_011512623.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510925.1 Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X3, mRNA.

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CloneID	OHu78358
Clone ID Related Accession (Same CDS sequence)	XM_011512623.2
Accession Version	XM_011512623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510925.1
CDS	369..5621
Translation	MKILLIIFVLIIWTETLADQSPGPGPVYADVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKDVTIFSVGVYNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLLDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDLGICVLVLGIGDVYKEHLLPITGNSEKIITFQDFDKLKNVDVKKRIIREICQSCGKTNCFMDIVVGFDISTHVQGQPLFQGHPQLESYLPGILEDISSIKGVSCGAGTEAQVSLAFKVNSDQGFPAKFQIYQKAVFDSLLQVNVSGPTHLNAQFLRSLWDTFKDKSASRGQVLLIFSDGLQSESNIMLENQSDRLREAGLDALLVVSLNTTAHHEFSSFEFGKRFDYRTHLTIGMRELGKKLSQYLGNIAERTCCCTFCKCPGIPGPHGTRGLQAMKGSQGLKGSRGHRGEDGNPGVRGDTGPQGDKGIAGCPGAWGQKGLKGFSGPKGGHGDDGIDGLDGEEGCHGFPGIKGEKGDPGSQGSPGSRGAPGQYGEKGFPGDPGNPGQNNNIKGQKGSKGEQGRQGRSGQKGVQGSPSSRGSRGREGQRGLRGVSGEPGNPGPTGTLGAEGLQGPQGSQGNPGRKGEKGSQGQKGPQGSPGLMGAKGSTGRPGLLGKKGEPGLPGDLGPVGQTGQRGRQGDSGIPGYGQMGRKGVKGPRGFPGDAGQKGDIGNPGIPGGPGPKGFRGLALTVGLKGEEGSRGLPGPPGQRGIKGMAGQPVYSQCDLIRFLREHSPCWKV

Target ORF information:

RefSeq Version	XM_011512623.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512623.2

ORF Insert Sequence:

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A5 ( NM_153264.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_694996.5 Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 2, mRNA.

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CloneID	OHu21032
Clone ID Related Accession (Same CDS sequence)	NM_153264.6
Accession Version	NM_153264.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	collagen alpha-5(VI) chain isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU085556.1, AK123718.1 and AI743837.1. This sequence is a reference standard in the RefSeqGene project. On May 23, 2013 this sequence version replaced NM_153264.5. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGAA AAATGTCCAG CATATCCAAC AGAGCTAGTA 
TTTGCTCTGG ACAATTCCTA CGATGTCACA GAAGAGAGCT TTAATAAAAC ACGGGACATC 
ATCACTTCCA TTGTCAATGA CCTTAACATC AGGGAAAATA ACTGTCCTGT GGGAGCAAGA 
GTTGCCATGG TTTCCTATAA CTCAGGCACC AGCTATCTCA TCCGCTGGTC TGACTACAAT 
AGGAAGAAGC AGCTCCTCCA GCAGCTTTCC CAAATAAAAT ATCAAGACAC CACAGAGCCC 
CGAGATGTTG GTAATGCAAT GAGGTTTGTG ACCCGCAACG TGTTCAAGCG GACGTATGCA 
GGAGCCAACG TGAGGAGAGT TGCTGTGTTT TTTAGCAATG GTCAAACAGC CAGTAGGTCA 
TCCATCATCA CGGCCACCAT GGAGTTTAGT GCCCTGGATA TCAGTCCAAC AGTCTTTGCT 
TTTGATGAGA GAGTTTTCCT TGAAGCTTTT GGGTTTGACA ACACTGGAAC ATTTCAGGTG 
ATTCCAGTTC CTCCAAATGG GGAAAATCAA ACATTAGAAA GACTTCGGCG CTGTGCACTT 
TGCTATGATA AATGTTTTCC AAATGCTTGC ATTCGAGAGG CTTTCTTACC TGAAGATTCA 
TACATGGATG TAGTCTTCCT CATAGACAAT TCTCGGAATA TAGCAAAGGA TGAGTTTAAG 
GCTGTGAAAG CCTTGGTGAG CTCAGTGATT GACAACTTCA ACATTGCTTC AGACCCTTTA 
ATCTCAGACT CTGGTGATAG GATTGCTTTG TTGAGCTATT CTCCTTGGGA AAGTTCCAGG 
AGAAAGATGG GTACAGTAAA AACAGAGTTT GATTTCATCA CTTATGACAA CCAACTCCTA 
ATGAAGAATC ACATCCAGAC TTCCTTCCAA CAGCTAAATG GAGAAGCAAC AATTGGTCGT 
GCCCTACTGT GGACCACTGA AAATCTTTTT CCAGAAACAC CCTATCTAAG AAAACACAAG 
GTCATCTTTG TGGTCTCAGC TGGAGAAAAT TATGAGAGAA AAGAATTTGT AAAAATGATG 
GCTTTGAGGG CTAAGTGTCA AGGCTACGTC ATATTTGTGA TTTCTCTGGG CTCTACACGT 
AAGGATGACA TGGAGGAGTT AGCCAGCTAC CCACTTGATC AACACCTGAT ACAGCTTGGG 
AGAATACATA AACCAGATCT GAATTATATT GCGAAGTTCT TAAAGCCATT TTTATACTCG 
GTCAGGCGGG GATTCAATCA GTACCCACCA CCGATGCTTG AGGATGCCTG TAGACTCATC 
AATTTAGGAG GAGAGAATAT TCAAAATGAT GGTTTCCAAT TTGTTACTGA GCTACAAGAG 
GATTTTTTGG GAGGTAATGG CTTCATTGGC CAAGAATTAA ATTCTGGGAG AGAATCACCT 
TTTGTAAAGA CGGAAGACAA TGGAAGTGAC TATTTGGTTT ACCTTCCAAG CCAAATGTTT 
GAGCCACAAA AATTAATGAT CAATTATGAA AAAGATCAAA AATCTGCAGA AATTGCAAGT 
CTCACTTCTG GACATGAAAA TTATGGCAGA AAAGAAGAAC CAGATCATAC TTATGAACCT 
GGAGATGTCT CTCTTCAAGA ATATTACATG GATGTGGCTT TCCTCATAGA TGCTTCCCAA 
AGAGTAGGAA GTGATGAGTT TAAGGAAGTA AAAGCTTTTA TAACCTCAGT GCTTGATTAC 
TTTCACATTG CCCCCACTCC ACTGACCTCC ACCTTAGGAG ACAGGGTTGC TGTCCTGAGC 
TACTCTCCTC CAGGCTATAT GCCTAACACT GAAGAATGCC CTGTCTACCT GGAATTTGAT 
TTGGTTACTT ATAACAGTAT ACACCAAATG AAACATCATC TCCAAGACTC TCAACAGCTC 
AATGGAGATG TTTTTATTGG CCATGCCTTG CAGTGGACAA TTGACAATGT CTTTGTAGGA 
ACCCCCAATC TGAGGAAAAA CAAAGTTATC TTTGTAATAT CTGCTGGCGA AACCAACTCT 
TTAGACAAAG ACGTCTTAAG GAATGTGTCT CTGAGAGCCA AGTGTCAAGG CTACTCCATA 
TTTGTGTTTT CCTTTGGCCC TAAACACAAT GACAAAGAAT TAGAAGAATT AGCCAGCCAC 
CCTCTGGATC ATCACTTGGT CCAACTTGGC CGAACCCACA AGCCAGATTG GAACTATATC 
ATCAAGTTTG TCAAGCCATT TGTCCATTTA ATCAGACGTG CCATCAACAA ATATCCCACC 
GAAGATATGA AAGCCACATG TGTTAACATG ACCTCTCCCA ACCCAGAGAA CGGTGGCACA 
GAAAACACTG TATTATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694996.5
CDS	495..8075
Translation	MKILLIIFVLIIWTETLADQSPGPGPVYADVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKDVTIFSVGVYNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLLDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDLGICVLVLGIGDVYKEHLLPITGNSEKIITFQDFDKLKNVDVKKRIIREICQSCGKTNCFMDIVVGFDISTHVQGQPLFQGHPQLESYLPGILEDISSIKGVSCGAGTEAQVSLAFKVNSDQGFPAKFQIYQKAVFDSLLQVNVSGPTHLNAQFLRSLWDTFKDKSASRGQVLLIFSDGLQSESNIMLENQSDRLREAGLDALLVVSLNTTAHHEFSSFEFGKRFDYRTHLTIGMRELGKKLSQYLGNIAERTCCCTFCKCPGIPGPHGTRGLQAMKGSQGLKGSRGHRGEDGNPGVRGDTGPQGDKGIAGCPGAWGQKGLKGFSGPKGGHGDDGIDGLDGEEGCHGFPGIKGEKGDPGSQGSPGSRGAPGQYGEKGFPGDPGNPGQNNNIKGQKGSKGEQGRQGRSGQKGVQGSPSSRGSRGREGQRGLRGVSGEPGNPGPTGTLGAEGLQGPQGSQGNPGRKGEKGSQGQKGPQGSPGLMGAKGSTGRPGLLGKKGEPGLPGDLGPVGQTGQRGRQGDSGIPGYGQMGRKGVKGPRGFPGDAGQKGDIGNPGIPGGPGPKGFRGLALTVGLKGEEGSRGLPGPPGQRGIKGMAGQPVYSQCDLIRFLREHSPCWKEKCPAYPTELVFALDNSYDVTEESFNKTRDIITSIVNDLNIRENNCPVGARVAMVSYNSGTSYLIRWSDYNRKKQLLQQLSQIKYQDTTEPRDVGNAMRFVTRNVFKRTYAGANVRRVAVFFSNGQTASRSSIITATMEFSALDISPTVFAFDERVFLEAFGFDNTGTFQVIPVPPNGENQTLERLRRCALCYDKCFPNACIREAFLPEDSYMDVVFLIDNSRNIAKDEFKAVKALVSSVIDNFNIASDPLISDSGDRIALLSYSPWESSRRKMGTVKTEFDFITYDNQLLMKNHIQTSFQQLNGEATIGRALLWTTENLFPETPYLRKHKVIFVVSAGENYERKEFVKMMALRAKCQGYVIFVISLGSTRKDDMEELASYPLDQHLIQLGRIHKPDLNYIAKFLKPFLYSVRRGFNQYPPPMLEDACRLINLGGENIQNDGFQFVTELQEDFLGGNGFIGQELNSGRESPFVKTEDNGSDYLVYLPSQMFEPQKLMINYEKDQKSAEIASLTSGHENYGRKEEPDHTYEPGDVSLQEYYMDVAFLIDASQRVGSDEFKEVKAFITSVLDYFHIAPTPLTSTLGDRVAVLSYSPPGYMPNTEECPVYLEFDLVTYNSIHQMKHHLQDSQQLNGDVFIGHALQWTIDNVFVGTPNLRKNKVIFVISAGETNSLDKDVLRNVSLRAKCQGYSIFVFSFGPKHNDKELEELASHPLDHHLVQLGRTHKPDWNYIIKFVKPFVHLIRRAINKYPTEDMKATCVNMTSPNPENGGTENTVLW

Target ORF information:

RefSeq Version	NM_153264.6
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153264.6

ORF Insert Sequence:

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGTTCT CCTGGGCTAA TGGGAGCTAA AGGGAGCACT 
GGAAGACCTG GACTTTTGGG GAAAAAAGGA GAGCCTGGAC TTCCTGGAGA TCTAGGGCCA 
GTGGGGCAAA CTGGGCAGCG AGGAAGACAG GGAGATTCTG GCATCCCAGG CTACGGTCAG 
ATGGGACGAA AAGGAGTAAA GGGCCCAAGA GGATTCCCTG GAGATGCGGG GCAGAAGGGT 
GATATTGGTA ATCCTGGAAT TCCTGGGGGA CCTGGACCCA AAGGATTTAG GGGACTAGCA 
CTCACTGTAG GCTTGAAAGG TGAAGAGGGA TCTCGAGGAC TCCCAGGCCC TCCTGGACAG 
AGAGGCATTA AAGGCATGGC AGGGCAGCCT GTATATTCTC AATGTGATCT GATCCGGTTT 
TTGCGGGAAC ATAGTCCTTG CTGGAAAGAA AAATGTCCAG CATATCCAAC AGAGCTAGTA 
TTTGCTCTGG ACAATTCCTA CGATGTCACA GAAGAGAGCT TTAATAAAAC ACGGGACATC 
ATCACTTCCA TTGTCAATGA CCTTAACATC AGGGAAAATA ACTGTCCTGT GGGAGCAAGA 
GTTGCCATGG TTTCCTATAA CTCAGGCACC AGCTATCTCA TCCGCTGGTC TGACTACAAT 
AGGAAGAAGC AGCTCCTCCA GCAGCTTTCC CAAATAAAAT ATCAAGACAC CACAGAGCCC 
CGAGATGTTG GTAATGCAAT GAGGTTTGTG ACCCGCAACG TGTTCAAGCG GACGTATGCA 
GGAGCCAACG TGAGGAGAGT TGCTGTGTTT TTTAGCAATG GTCAAACAGC CAGTAGGTCA 
TCCATCATCA CGGCCACCAT GGAGTTTAGT GCCCTGGATA TCAGTCCAAC AGTCTTTGCT 
TTTGATGAGA GAGTTTTCCT TGAAGCTTTT GGGTTTGACA ACACTGGAAC ATTTCAGGTG 
ATTCCAGTTC CTCCAAATGG GGAAAATCAA ACATTAGAAA GACTTCGGCG CTGTGCACTT 
TGCTATGATA AATGTTTTCC AAATGCTTGC ATTCGAGAGG CTTTCTTACC TGAAGATTCA 
TACATGGATG TAGTCTTCCT CATAGACAAT TCTCGGAATA TAGCAAAGGA TGAGTTTAAG 
GCTGTGAAAG CCTTGGTGAG CTCAGTGATT GACAACTTCA ACATTGCTTC AGACCCTTTA 
ATCTCAGACT CTGGTGATAG GATTGCTTTG TTGAGCTATT CTCCTTGGGA AAGTTCCAGG 
AGAAAGATGG GTACAGTAAA AACAGAGTTT GATTTCATCA CTTATGACAA CCAACTCCTA 
ATGAAGAATC ACATCCAGAC TTCCTTCCAA CAGCTAAATG GAGAAGCAAC AATTGGTCGT 
GCCCTACTGT GGACCACTGA AAATCTTTTT CCAGAAACAC CCTATCTAAG AAAACACAAG 
GTCATCTTTG TGGTCTCAGC TGGAGAAAAT TATGAGAGAA AAGAATTTGT AAAAATGATG 
GCTTTGAGGG CTAAGTGTCA AGGCTACGTC ATATTTGTGA TTTCTCTGGG CTCTACACGT 
AAGGATGACA TGGAGGAGTT AGCCAGCTAC CCACTTGATC AACACCTGAT ACAGCTTGGG 
AGAATACATA AACCAGATCT GAATTATATT GCGAAGTTCT TAAAGCCATT TTTATACTCG 
GTCAGGCGGG GATTCAATCA GTACCCACCA CCGATGCTTG AGGATGCCTG TAGACTCATC 
AATTTAGGAG GAGAGAATAT TCAAAATGAT GGTTTCCAAT TTGTTACTGA GCTACAAGAG 
GATTTTTTGG GAGGTAATGG CTTCATTGGC CAAGAATTAA ATTCTGGGAG AGAATCACCT 
TTTGTAAAGA CGGAAGACAA TGGAAGTGAC TATTTGGTTT ACCTTCCAAG CCAAATGTTT 
GAGCCACAAA AATTAATGAT CAATTATGAA AAAGATCAAA AATCTGCAGA AATTGCAAGT 
CTCACTTCTG GACATGAAAA TTATGGCAGA AAAGAAGAAC CAGATCATAC TTATGAACCT 
GGAGATGTCT CTCTTCAAGA ATATTACATG GATGTGGCTT TCCTCATAGA TGCTTCCCAA 
AGAGTAGGAA GTGATGAGTT TAAGGAAGTA AAAGCTTTTA TAACCTCAGT GCTTGATTAC 
TTTCACATTG CCCCCACTCC ACTGACCTCC ACCTTAGGAG ACAGGGTTGC TGTCCTGAGC 
TACTCTCCTC CAGGCTATAT GCCTAACACT GAAGAATGCC CTGTCTACCT GGAATTTGAT 
TTGGTTACTT ATAACAGTAT ACACCAAATG AAACATCATC TCCAAGACTC TCAACAGCTC 
AATGGAGATG TTTTTATTGG CCATGCCTTG CAGTGGACAA TTGACAATGT CTTTGTAGGA 
ACCCCCAATC TGAGGAAAAA CAAAGTTATC TTTGTAATAT CTGCTGGCGA AACCAACTCT 
TTAGACAAAG ACGTCTTAAG GAATGTGTCT CTGAGAGCCA AGTGTCAAGG CTACTCCATA 
TTTGTGTTTT CCTTTGGCCC TAAACACAAT GACAAAGAAT TAGAAGAATT AGCCAGCCAC 
CCTCTGGATC ATCACTTGGT CCAACTTGGC CGAACCCACA AGCCAGATTG GAACTATATC 
ATCAAGTTTG TCAAGCCATT TGTCCATTTA ATCAGACGTG CCATCAACAA ATATCCCACC 
GAAGATATGA AAGCCACATG TGTTAACATG ACCTCTCCCA ACCCAGAGAA CGGTGGCACA 
GAAAACACTG TATTATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A5 ( XM_011512621.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510923.1 Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X1, mRNA.

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CloneID	OHu78356
Clone ID Related Accession (Same CDS sequence)	XM_011512621.2
Accession Version	XM_011512621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-5(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGAGAG CCTGGACTTC CTGGAGATCT AGGGCCAGTG 
GGGCAAACTG GGCAGCGAGG AAGACAGGGA GATTCTGGCA TCCCAGGCTA CGGTCAGATG 
GGACGAAAAG GAGTAAAGGG CCCAAGAGGA TTCCCTGGAG ATGCGGGGCA GAAGGGTGAT 
ATTGGTAATC CTGGAATTCC TGGGGGACCT GGACCCAAAG GATTTAGGGG ACTAGCACTC 
ACTGTAGGCT TGAAAGGTGA AGAGGGATCT CGAGGACTCC CAGGCCCTCC TGGACAGAGA 
GGCATTAAAG GCATGGCAGG GCAGCCTGTA TATTCTCAAT GTGATCTGAT CCGGTTTTTG 
CGGGAACATA GTCCTTGCTG GAAAGAAAAA TGTCCAGCAT ATCCAACAGA GCTAGTATTT 
GCTCTGGACA ATTCCTACGA TGTCACAGAA GAGAGCTTTA ATAAAACACG GGACATCATC 
ACTTCCATTG TCAATGACCT TAACATCAGG GAAAATAACT GTCCTGTGGG AGCAAGAGTT 
GCCATGGTTT CCTATAACTC AGGCACCAGC TATCTCATCC GCTGGTCTGA CTACAATAGG 
AAGAAGCAGC TCCTCCAGCA GCTTTCCCAA ATAAAATATC AAGACACCAC AGAGCCCCGA 
GATGTTGGTA ATGCAATGAG GTTTGTGACC CGCAACGTGT TCAAGCGGAC GTATGCAGGA 
GCCAACGTGA GGAGAGTTGC TGTGTTTTTT AGCAATGGTC AAACAGCCAG TAGGTCATCC 
ATCATCACGG CCACCATGGA GTTTAGTGCC CTGGATATCA GTCCAACAGT CTTTGCTTTT 
GATGAGAGAG TTTTCCTTGA AGCTTTTGGG TTTGACAACA CTGGAACATT TCAGGTGATT 
CCAGTTCCTC CAAATGGGGA AAATCAAACA TTAGAAAGAC TTCGGCGCTG TGCACTTTGC 
TATGATAAAT GTTTTCCAAA TGCTTGCATT CGAGAGGCTT TCTTACCTGA AGATTCATAC 
ATGGATGTAG TCTTCCTCAT AGACAATTCT CGGAATATAG CAAAGGATGA GTTTAAGGCT 
GTGAAAGCCT TGGTGAGCTC AGTGATTGAC AACTTCAACA TTGCTTCAGA CCCTTTAATC 
TCAGACTCTG GTGATAGGAT TGCTTTGTTG AGCTATTCTC CTTGGGAAAG TTCCAGGAGA 
AAGATGGGTA CAGTAAAAAC AGAGTTTGAT TTCATCACTT ATGACAACCA ACTCCTAATG 
AAGAATCACA TCCAGACTTC CTTCCAACAG CTAAATGGAG AAGCAACAAT TGGTCGTGCC 
CTACTGTGGA CCACTGAAAA TCTTTTTCCA GAAACACCCT ATCTAAGAAA ACACAAGGTC 
ATCTTTGTGG TCTCAGCTGG AGAAAATTAT GAGAGAAAAG AATTTGTAAA AATGATGGCT 
TTGAGGGCTA AGTGTCAAGG CTACGTCATA TTTGTGATTT CTCTGGGCTC TACACGTAAG 
GATGACATGG AGGAGTTAGC CAGCTACCCA CTTGATCAAC ACCTGATACA GCTTGGGAGA 
ATACATAAAC CAGATCTGAA TTATATTGCG AAGTTCTTAA AGCCATTTTT ATACTCGGTC 
AGGCGGGGAT TCAATCAGTA CCCACCACCG ATGCTTGAGG ATGCCTGTAG ACTCATCAAT 
TTAGGAGGAG AGAATATTCA AAATGATGGT TTCCAATTTG TTACTGAGCT ACAAGAGGAT 
TTTTTGGGAG GTAATGGCTT CATTGGCCAA GAATTAAATT CTGGGAGAGA ATCACCTTTT 
GTAAAGACGG AAGACAATGG AAGTGACTAT TTGGTTTACC TTCCAAGCCA AATGTTTGAG 
CCACAAAAAT TAATGATCAA TTATGAAAAA GATCAAAAAT CTGCAGAAAT TGCAAGTCTC 
ACTTCTGGAC ATGAAAATTA TGGCAGAAAA GAAGAACCAG ATCATACTTA TGAACCTGGA 
GATGTCTCTC TTCAAGAATA TTACATGGAT GTGGCTTTCC TCATAGATGC TTCCCAAAGA 
GTAGGAAGTG ATGAGTTTAA GGAAGTAAAA GCTTTTATAA CCTCAGTGCT TGATTACTTT 
CACATTGCCC CCACTCCACT GACCTCCACC TTAGGAGACA GGGTTGCTGT CCTGAGCTAC 
TCTCCTCCAG GCTATATGCC TAACACTGAA GAATGCCCTG TCTACCTGGA ATTTGATTTG 
GTTACTTATA ACAGTATACA CCAAATGAAA CATCATCTCC AAGACTCTCA ACAGCTCAAT 
GGAGATGTTT TTATTGGCCA TGCCTTGCAG TGGACAATTG ACAATGTCTT TGTAGGAACC 
CCCAATCTGA GGAAAAACAA AGTTATCTTT GTAATATCTG CTGGCGAAAC CAACTCTTTA 
GACAAAGACG TCTTAAGGAA TGTGTCTCTG AGAGCCAAGT GTCAAGGCTA CTCCATATTT 
GTGTTTTCCT TTGGCCCTAA ACACAATGAC AAAGAATTAG AAGAATTAGC CAGCCACCCT 
CTGGATCATC ACTTGGTCCA ACTTGGCCGA ACCCACAAGC CAGATTGGAA CTATATCATC 
AAGTTTGTCA AGCCATTTGT CCATTTAATC AGACGTGCCA TCAACAAATA TCCCACCGAA 
GATATGAAAG CCACATGTGT TAACATGACC TCTCCCAACC CAGAGAACGG TGGCACAGAA 
AACACTGTAT TACTTCTTCC TGGGATATAT GAAATAAAGA CAGAAAATGG TGATCTGTTT 
GATGAATTTG ATTCCCAGGC ACAACATCTC CTTGTATTAG GGAACAATCA TAGTAGTGGT 
TCTGAGACAG CTACTGATTT GATGCAGAAG TTATATCTGC TCTTTTCAAC TGAGAAACTG 
GCAATGAAAG ACAAGGAAAA AGCACATTTA GAAGAAATTT CAGCTCTAGT GGTTGATAAA 
CAGCAAGAAA AAGAAGATAA GGAAATGGAA GCTACAGACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510923.1
CDS	369..8153
Translation	MKILLIIFVLIIWTETLADQSPGPGPVYADVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKDVTIFSVGVYNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLLDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDLGICVLVLGIGDVYKEHLLPITGNSEKIITFQDFDKLKNVDVKKRIIREICQSCGKTNCFMDIVVGFDISTHVQGQPLFQGHPQLESYLPGILEDISSIKGVSCGAGTEAQVSLAFKVNSDQGFPAKFQIYQKAVFDSLLQVNVSGPTHLNAQFLRSLWDTFKDKSASRGQVLLIFSDGLQSESNIMLENQSDRLREAGLDALLVVSLNTTAHHEFSSFEFGKRFDYRTHLTIGMRELGKKLSQYLGNIAERTCCCTFCKCPGIPGPHGTRGLQAMKGSQGLKGSRGHRGEDGNPGVRGDTGPQGDKGIAGCPGAWGQKGLKGFSGPKGGHGDDGIDGLDGEEGCHGFPGIKGEKGDPGSQGSPGSRGAPGQYGEKGFPGDPGNPGQNNNIKGQKGSKGEQGRQGRSGQKGVQGSPSSRGSRGREGQRGLRGVSGEPGNPGPTGTLGAEGLQGPQGSQGNPGRKGEKGSQGQKGPQGEPGLPGDLGPVGQTGQRGRQGDSGIPGYGQMGRKGVKGPRGFPGDAGQKGDIGNPGIPGGPGPKGFRGLALTVGLKGEEGSRGLPGPPGQRGIKGMAGQPVYSQCDLIRFLREHSPCWKEKCPAYPTELVFALDNSYDVTEESFNKTRDIITSIVNDLNIRENNCPVGARVAMVSYNSGTSYLIRWSDYNRKKQLLQQLSQIKYQDTTEPRDVGNAMRFVTRNVFKRTYAGANVRRVAVFFSNGQTASRSSIITATMEFSALDISPTVFAFDERVFLEAFGFDNTGTFQVIPVPPNGENQTLERLRRCALCYDKCFPNACIREAFLPEDSYMDVVFLIDNSRNIAKDEFKAVKALVSSVIDNFNIASDPLISDSGDRIALLSYSPWESSRRKMGTVKTEFDFITYDNQLLMKNHIQTSFQQLNGEATIGRALLWTTENLFPETPYLRKHKVIFVVSAGENYERKEFVKMMALRAKCQGYVIFVISLGSTRKDDMEELASYPLDQHLIQLGRIHKPDLNYIAKFLKPFLYSVRRGFNQYPPPMLEDACRLINLGGENIQNDGFQFVTELQEDFLGGNGFIGQELNSGRESPFVKTEDNGSDYLVYLPSQMFEPQKLMINYEKDQKSAEIASLTSGHENYGRKEEPDHTYEPGDVSLQEYYMDVAFLIDASQRVGSDEFKEVKAFITSVLDYFHIAPTPLTSTLGDRVAVLSYSPPGYMPNTEECPVYLEFDLVTYNSIHQMKHHLQDSQQLNGDVFIGHALQWTIDNVFVGTPNLRKNKVIFVISAGETNSLDKDVLRNVSLRAKCQGYSIFVFSFGPKHNDKELEELASHPLDHHLVQLGRTHKPDWNYIIKFVKPFVHLIRRAINKYPTEDMKATCVNMTSPNPENGGTENTVLLLPGIYEIKTENGDLFDEFDSQAQHLLVLGNNHSSGSETATDLMQKLYLLFSTEKLAMKDKEKAHLEEISALVVDKQQEKEDKEMEATDI

Target ORF information:

RefSeq Version	XM_011512621.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 5 chain (COL6A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512621.2

ORF Insert Sequence:

ATGAAGATCT TGCTAATTAT ATTTGTCCTA ATCATTTGGA CTGAAACATT GGCAGACCAG 
AGCCCAGGGC CAGGCCCTGT GTATGCAGAT GTCGTGTTTC TGGTGGACAG CTCCGATCAC 
CTGGGACCTA AGTCATTCCC ATTCGTGAAA ACGTTCATCA ACAAAATGAT CAACAGTCTC 
CCCATAGAGG CCAACAAATA CCGTGTAGCC CTGGCCCAGT ACAGCGACGA GTTTCACAGT 
GAATTCCATC TGAGCACCTT CAAAGGCAGA AGCCCCATGC TGAACCACCT CAAGAAGAAC 
TTTCAGTTCA TTGGCGGGTC CCTGCAGATA GGAAAGGCTC TTCAGGAGGC TCATAGGACC 
TACTTCTCTG CACCCATAAA TGGGAGAGAC AGGAAACAGT TTCCCCCAAT TTTGGTGGTC 
CTGGCTTCGG CTGAGTCTGA GGATGAAGTG GAAGAGGCTT CGAAAGCCCT GCAGAAAGAC 
GGGGTGAAAA TTATCTCCGT GGGGGTGCAG AAAGCTTCTG AGGAAAATCT GAAGGCCATG 
GCCACATCCC ATTTCCATTT CAACCTTCGG ACAATCAGAG ACCTCAGCAC ATTTTCCCAA 
AACATGACAC AGATCATCAA GGATGTAACC AAGTATAAGG AGGGAGCCGT CGATGCTGAT 
ATGCAAGTTC ACTTCCCCAT ATCCTGTCAG AAAGATTCAC TCGCTGACCT CGTGTTCCTG 
GTGGATGAGT CACTTGGGAC CGGAGGAAAT TTAAGGCATC TTCAGACCTT CCTTGAGAAC 
ATTACCAGCT CCATGGATGT GAAGGAAAAT TGCATGCGAC TTGGACTGAT GAGTTACAGC 
AATAGTGCCA AGACTATTTC TTTTCTTAAA TCAAGCACAA CCCAATCTGA ATTTCAGCAG 
CAAATCAAGA ATCTTTCTAT CCAAGTTGGG AAATCCAATA CAGGGGCTGC CATTGATCAG 
ATGAGAAGAG ACGGCTTCTC AGAGTCATAT GGCAGCAGAA GAGCACAAGG AGTGCCTCAG 
ATTGCAGTTC TGGTCACCCA CAGACCATCA GATGATGAGG TGCATGATGC TGCGCTGAAC 
CTTCGACTGG AGGATGTAAA CGTGTTTGCC TTGAGCATCC AAGGGGCTAA CAATACCCAG 
TTAGAAGAAA TAGTGTCTTA TCCTCCAGAA CAGACAATTT CCACGCTGAA GTCCTATGCA 
GACTTAGAAA CTTACAGTAC AAAGTTCCTG AAAAAGCTCC AGAATGAAAT ATGGTCCCAA 
ATTTCTACTT ATGCTGAACA AAGGAATCTT GATAAAACTG GCTGTGTGGA TACAAAAGAG 
GCTGATATCC ACTTCCTCAT TGATGGCTCA AGCAGCATCC AGGAGAAACA GTTTGAGCAA 
ATCAAGAGAT TTATGTTGGA AGTGACAGAA ATGTTTAGCA TTGGCCCAGA CAAAGTCCGA 
GTTGGAGTTG TGCAGTATTC AGATGACACA GAAGTGGAAT TTTATATCAC TGACTATTCT 
AATGATATTG ACTTAAGAAA GGCTATTTTT AACATTAAGC AACTAACTGG TGGAACTTAT 
ACTGGGAAAG CTCTGGATTA CATACTGCAA ATAATAAAAA ATGGAATGAA GGATAGAATG 
AGCAAGGTTC CCTGTTACCT CATTGTGTTG ACTGATGGGA TGTCCACAGA CAGAGTCGTG 
GAACCTGCTA AGAGACTAAG GGCTGAGCAA ATCACTGTTC ATGCAGTTGG CATTGGGGCA 
GCTAATAAAA TAGAACTGCA AGAAATTGCT GGGAAAGAAG AAAGGGTTAG CTTTGGGCAG 
AACTTTGATG CTTTGAAAAG CATAAAAAAT GAAGTCGTTC GTGAAATCTG CGCTGAAAAA 
GGATGTGAAG ACATGAAGGC CGACATCATG TTTCTGGTGG ACAGTTCTTG GAGTATAGGA 
AATGAAAATT TTAGGAAAAT GAAAATCTTC ATGAAAAACC TGTTAACTAA AATTCAAATT 
GGTGCAGACA AAACCCAGAT TGGTGTTGTT CAGTTCAGTG ATAAAACTAA GGAAGAGTTC 
CAGCTTAATA GATATTTTAC ACAGCAAGAA ATTTCTGATG CAATAGATAG AATGTCTCTC 
ATCAATGAAG GCACTTTAAC TGGAAAGGCA CTAAATTTTG TAGGTCAATA CTTCACCCAC 
TCCAAGGGGG CCCGTTTGGG GGCCAAAAAA TTTCTCATCC TCATCACAGA TGGAGTAGCG 
CAGGATGATG TGAGAGATCC TGCTAGAATT CTTCGGGGCA AAGATGTGAC CATCTTCTCT 
GTAGGAGTAT ACAATGCCAA TAGATCTCAG CTAGAAGAGA TCAGTGGGGA TAGCAGCCTA 
GTTTTTCATG TTGAGAACTT CGATCATCTA AAGGCACTAG AAAGGAAACT TATCTTTCGT 
GTGTGTGCTC TCCATGATTG TAAAAGGATT ACACTACTAG ACGTTGTGTT TGTGCTGGAT 
CATTCAGGTA GCATAAAAAA ACAATATCAA GATCACATGA TTAACCTAAC TATCCATTTG 
GTGAAGAAAG CAGATGTTGG CAGGGACCGA GTTCAGTTTG GAGCCCTCAA ATACTCTGAC 
CAACCTAACA TCCTTTTCTA CCTTAATACA TACTCGAACA GATCAGCAAT AATTGAGAAT 
CTGCGGAAGC GCAGGGACAC TGGAGGGAAC ACCTACACTG CCAAGGCTCT CAAGCACGCA 
AATGCCCTGT TTACAGAGGA ACATGGCAGC CGCATCAAGC AAAATGTGAA GCAGATGCTG 
ATTGTCATCA CCGATGGGGA ATCCCATGAC CATGATCAGC TCAATGACAC AGCATTGGAA 
CTGAGAAACA AAGGCATCAC CATCTTTGCA GTGGGTGTAG GAAAGGCCAA CCAAAAGGAA 
CTTGAGGGTA TGGCAGGGAA TAAAAACAAT ACTATCTATG TAGATAATTT TGACAAACTG 
AAAGATGTTT TCACACTTGT TCAAGAACGT ATGTGTACTG AAGCACCAGA GGTCTGTCAT 
CTTCAGGAAG CTGACGTGAT TTTCCTTTGC GATGGCTCTG ACAGGGTATC TAATTCAGAT 
TTTGTAACCA TGACAACTTT CTTGTCAGAC TTAATCGATA ATTTTGACAT TCAGTCTCAA 
AGAATGAAAA TTGGTATGGC TCAATTTGGA AGCAACTACC AGAGTATTAT TGAGTTGAAA 
AACTCTCTGA CTAAAACCCA GTGGAAGACT CAAATTCAGA ATGTCTCCAA GAGCGGTGGA 
TTTCCAAGAA TTGACTTTGC CCTTAAAAAA GTGAGCAATA TGTTTAATCT ACATGCTGGT 
GGGAGAAGAA ATGCTGGTGT CCCCCAAACT TTGGTTGTTA TCACATCTGG AGATCCTCGC 
TATGATGTGG CAGATGCAGT AAAAACCCTG AAGGACCTTG GAATTTGTGT CCTGGTTTTG 
GGCATAGGAG ATGTTTATAA GGAACATCTC CTGCCAATAA CAGGCAATTC TGAAAAAATA 
ATCACTTTTC AAGACTTTGA TAAATTAAAG AATGTGGATG TGAAAAAAAG AATCATCCGT 
GAAATCTGCC AGAGCTGTGG GAAAACCAAT TGCTTTATGG ACATAGTGGT TGGGTTTGAC 
ATCTCCACTC ATGTGCAGGG GCAGCCTTTG TTCCAGGGCC ACCCCCAGCT GGAATCCTAC 
CTCCCAGGCA TCTTAGAAGA CATCAGCTCT ATCAAGGGGG TGAGCTGTGG GGCTGGCACA 
GAGGCACAGG TGAGCTTGGC GTTTAAGGTG AACAGTGACC AAGGATTCCC TGCCAAGTTC 
CAAATCTATC AGAAAGCAGT GTTTGACAGC TTGCTGCAAG TCAACGTCAG TGGGCCAACT 
CATCTGAACG CACAGTTCTT GCGGTCTCTT TGGGACACAT TTAAGGATAA ATCTGCATCC 
CGGGGCCAGG TGCTGCTTAT TTTTTCAGAC GGTCTCCAGA GTGAAAGCAA CATAATGCTT 
GAAAATCAAT CAGACAGGCT CAGAGAAGCA GGACTTGATG CTCTGCTGGT AGTGTCCCTT 
AACACAACTG CTCATCATGA GTTTTCTAGC TTTGAATTTG GAAAAAGATT CGATTACAGG 
ACTCATCTGA CTATTGGAAT GAGAGAACTG GGCAAAAAAC TATCACAGTA CCTGGGAAAT 
ATTGCAGAGA GGACTTGCTG CTGTACATTC TGCAAATGTC CAGGAATTCC AGGACCTCAT 
GGGACCCGAG GACTACAAGC CATGAAGGGT TCTCAAGGTC TGAAAGGCAG CAGAGGACAC 
AGGGGAGAGG ATGGAAACCC TGGAGTACGA GGAGACACAG GACCCCAAGG AGACAAAGGG 
ATTGCAGGAT GTCCAGGGGC GTGGGGTCAG AAGGGACTCA AAGGATTTTC TGGACCTAAG 
GGAGGTCATG GAGACGATGG GATTGATGGA CTTGATGGGG AAGAGGGCTG TCATGGATTT 
CCTGGAATAA AAGGAGAAAA AGGTGATCCA GGATCTCAGG GCAGCCCAGG TTCCAGAGGT 
GCCCCTGGGC AGTATGGAGA GAAGGGCTTC CCAGGGGATC CGGGTAATCC AGGACAAAAC 
AATAACATCA AAGGACAAAA GGGCTCCAAA GGAGAACAAG GAAGACAAGG TAGAAGTGGA 
CAGAAAGGGG TGCAAGGCAG TCCTAGTTCC AGAGGCAGCA GGGGAAGAGA AGGTCAAAGG 
GGACTCCGAG GTGTCTCAGG AGAACCAGGA AATCCTGGAC CTACAGGCAC ATTGGGAGCT 
GAAGGATTAC AAGGCCCACA GGGGTCACAG GGAAATCCTG GCAGAAAAGG AGAAAAAGGA 
AGCCAGGGGC AGAAAGGACC TCAGGGAGAG CCTGGACTTC CTGGAGATCT AGGGCCAGTG 
GGGCAAACTG GGCAGCGAGG AAGACAGGGA GATTCTGGCA TCCCAGGCTA CGGTCAGATG 
GGACGAAAAG GAGTAAAGGG CCCAAGAGGA TTCCCTGGAG ATGCGGGGCA GAAGGGTGAT 
ATTGGTAATC CTGGAATTCC TGGGGGACCT GGACCCAAAG GATTTAGGGG ACTAGCACTC 
ACTGTAGGCT TGAAAGGTGA AGAGGGATCT CGAGGACTCC CAGGCCCTCC TGGACAGAGA 
GGCATTAAAG GCATGGCAGG GCAGCCTGTA TATTCTCAAT GTGATCTGAT CCGGTTTTTG 
CGGGAACATA GTCCTTGCTG GAAAGAAAAA TGTCCAGCAT ATCCAACAGA GCTAGTATTT 
GCTCTGGACA ATTCCTACGA TGTCACAGAA GAGAGCTTTA ATAAAACACG GGACATCATC 
ACTTCCATTG TCAATGACCT TAACATCAGG GAAAATAACT GTCCTGTGGG AGCAAGAGTT 
GCCATGGTTT CCTATAACTC AGGCACCAGC TATCTCATCC GCTGGTCTGA CTACAATAGG 
AAGAAGCAGC TCCTCCAGCA GCTTTCCCAA ATAAAATATC AAGACACCAC AGAGCCCCGA 
GATGTTGGTA ATGCAATGAG GTTTGTGACC CGCAACGTGT TCAAGCGGAC GTATGCAGGA 
GCCAACGTGA GGAGAGTTGC TGTGTTTTTT AGCAATGGTC AAACAGCCAG TAGGTCATCC 
ATCATCACGG CCACCATGGA GTTTAGTGCC CTGGATATCA GTCCAACAGT CTTTGCTTTT 
GATGAGAGAG TTTTCCTTGA AGCTTTTGGG TTTGACAACA CTGGAACATT TCAGGTGATT 
CCAGTTCCTC CAAATGGGGA AAATCAAACA TTAGAAAGAC TTCGGCGCTG TGCACTTTGC 
TATGATAAAT GTTTTCCAAA TGCTTGCATT CGAGAGGCTT TCTTACCTGA AGATTCATAC 
ATGGATGTAG TCTTCCTCAT AGACAATTCT CGGAATATAG CAAAGGATGA GTTTAAGGCT 
GTGAAAGCCT TGGTGAGCTC AGTGATTGAC AACTTCAACA TTGCTTCAGA CCCTTTAATC 
TCAGACTCTG GTGATAGGAT TGCTTTGTTG AGCTATTCTC CTTGGGAAAG TTCCAGGAGA 
AAGATGGGTA CAGTAAAAAC AGAGTTTGAT TTCATCACTT ATGACAACCA ACTCCTAATG 
AAGAATCACA TCCAGACTTC CTTCCAACAG CTAAATGGAG AAGCAACAAT TGGTCGTGCC 
CTACTGTGGA CCACTGAAAA TCTTTTTCCA GAAACACCCT ATCTAAGAAA ACACAAGGTC 
ATCTTTGTGG TCTCAGCTGG AGAAAATTAT GAGAGAAAAG AATTTGTAAA AATGATGGCT 
TTGAGGGCTA AGTGTCAAGG CTACGTCATA TTTGTGATTT CTCTGGGCTC TACACGTAAG 
GATGACATGG AGGAGTTAGC CAGCTACCCA CTTGATCAAC ACCTGATACA GCTTGGGAGA 
ATACATAAAC CAGATCTGAA TTATATTGCG AAGTTCTTAA AGCCATTTTT ATACTCGGTC 
AGGCGGGGAT TCAATCAGTA CCCACCACCG ATGCTTGAGG ATGCCTGTAG ACTCATCAAT 
TTAGGAGGAG AGAATATTCA AAATGATGGT TTCCAATTTG TTACTGAGCT ACAAGAGGAT 
TTTTTGGGAG GTAATGGCTT CATTGGCCAA GAATTAAATT CTGGGAGAGA ATCACCTTTT 
GTAAAGACGG AAGACAATGG AAGTGACTAT TTGGTTTACC TTCCAAGCCA AATGTTTGAG 
CCACAAAAAT TAATGATCAA TTATGAAAAA GATCAAAAAT CTGCAGAAAT TGCAAGTCTC 
ACTTCTGGAC ATGAAAATTA TGGCAGAAAA GAAGAACCAG ATCATACTTA TGAACCTGGA 
GATGTCTCTC TTCAAGAATA TTACATGGAT GTGGCTTTCC TCATAGATGC TTCCCAAAGA 
GTAGGAAGTG ATGAGTTTAA GGAAGTAAAA GCTTTTATAA CCTCAGTGCT TGATTACTTT 
CACATTGCCC CCACTCCACT GACCTCCACC TTAGGAGACA GGGTTGCTGT CCTGAGCTAC 
TCTCCTCCAG GCTATATGCC TAACACTGAA GAATGCCCTG TCTACCTGGA ATTTGATTTG 
GTTACTTATA ACAGTATACA CCAAATGAAA CATCATCTCC AAGACTCTCA ACAGCTCAAT 
GGAGATGTTT TTATTGGCCA TGCCTTGCAG TGGACAATTG ACAATGTCTT TGTAGGAACC 
CCCAATCTGA GGAAAAACAA AGTTATCTTT GTAATATCTG CTGGCGAAAC CAACTCTTTA 
GACAAAGACG TCTTAAGGAA TGTGTCTCTG AGAGCCAAGT GTCAAGGCTA CTCCATATTT 
GTGTTTTCCT TTGGCCCTAA ACACAATGAC AAAGAATTAG AAGAATTAGC CAGCCACCCT 
CTGGATCATC ACTTGGTCCA ACTTGGCCGA ACCCACAAGC CAGATTGGAA CTATATCATC 
AAGTTTGTCA AGCCATTTGT CCATTTAATC AGACGTGCCA TCAACAAATA TCCCACCGAA 
GATATGAAAG CCACATGTGT TAACATGACC TCTCCCAACC CAGAGAACGG TGGCACAGAA 
AACACTGTAT TACTTCTTCC TGGGATATAT GAAATAAAGA CAGAAAATGG TGATCTGTTT 
GATGAATTTG ATTCCCAGGC ACAACATCTC CTTGTATTAG GGAACAATCA TAGTAGTGGT 
TCTGAGACAG CTACTGATTT GATGCAGAAG TTATATCTGC TCTTTTCAAC TGAGAAACTG 
GCAATGAAAG ACAAGGAAAA AGCACATTTA GAAGAAATTT CAGCTCTAGT GGTTGATAAA 
CAGCAAGAAA AAGAAGATAA GGAAATGGAA GCTACAGACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Longitudinal exome-wide association study to identify genetic susceptibility loci for hypertension in a Japanese population.
Experimental & molecular medicine49(12)e409;(2017 Dec)
Yasukochi Y,Sakuma J,Takeuchi I,Kato K,Oguri M,Fujimaki T,Horibe H,Yamada Y

Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.
Leukemia31(11)2426-2434(2017 Nov)
Glavey SV,Naba A,Manier S,Clauser K,Tahri S,Park J,Reagan MR,Moschetta M,Mishima Y,Gambella M,Rocci A,Sacco A,O'Dwyer ME,Asara JM,Palumbo A,Roccaro AM,Hynes RO,Ghobrial IM

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

COL6A5 variants in familial neuropathic chronic itch.
Brain : a journal of neurology140(3)555-567(2017 Mar)
Martinelli-Boneschi F,Colombi M,Castori M,Devigili G,Eleopra R,Malik RA,Ritelli M,Zoppi N,Dordoni C,Sorosina M,Grammatico P,Fadavi H,Gerrits MM,Almomani R,Faber CG,Merkies IS,Toniolo D,,Cocca M,Doglioni C,Waxman SG,Dib-Hajj SD,Taiana MM,Sassone J,Lombardi R,Cazzato D,Zauli A,Santoro S,Marchi M,Lauria G

EMR-linked GWAS study: investigation of variation landscape of loci for body mass index in children.
Frontiers in genetics4268(2013)
Namjou B,Keddache M,Marsolo K,Wagner M,Lingren T,Cobb B,Perry C,Kennebeck S,Holm IA,Li R,Crimmins NA,Martin L,Solti I,Kohane IS,Harley JB

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COL6A5 cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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