GAA cDNA ORF clone, Homo sapiens(human)

The following GAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16152 NM_000152.5
Latest version!
Homo sapiens glucosidase alpha, acid (GAA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_001079804.3
Latest version!
Homo sapiens glucosidase alpha, acid (GAA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_001079803.3
Latest version!
Homo sapiens glucosidase alpha, acid (GAA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 XM_005257194.4
Latest version!
Homo sapiens glucosidase alpha, acid (GAA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 XM_005257193.2
Latest version!
Homo sapiens glucosidase alpha, acid (GAA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
View Old Accession Versions >>
OHu16152 NM_001079803.1 Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_000152.3 Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_001079804.1 Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_001079803.2 Homo sapiens glucosidase alpha, acid (GAA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_001079804.2 Homo sapiens glucosidase alpha, acid (GAA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 XM_005257193.1 Homo sapiens glucosidase, alpha; acid (GAA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 XM_005257194.3 Homo sapiens glucosidase, alpha; acid (GAA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu16152 NM_000152.4 Homo sapiens glucosidase alpha, acid (GAA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_000152.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000143.2
    Translation

    Target ORF information:

    RefSeq Version NM_000152.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000152.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079803.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073271.1
    Translation

    Target ORF information:

    RefSeq Version NM_001079803.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079803.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY101855.1, BC040431.1, BF206270.1, M34424.1, BQ711289.1 and BF344248.1. On May 31, 2019 this sequence version replaced NM_001079803.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1783113.1, SRR7346977.1584163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073271.1
    CDS158..3016
    Translation

    Target ORF information:

    RefSeq Version NM_001079803.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079803.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079804.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC392486.1, BF206270.1, M34424.1, BQ711289.1, BC040431.1 and BF344248.1. On May 31, 2019 this sequence version replaced NM_001079804.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1771713.1, SRR1803613.175893.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073272.1
    CDS78..2936
    Translation

    Target ORF information:

    RefSeq Version NM_001079804.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079804.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_000152.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY101855.1, HY047261.1, HY015584.1, Y00839.1, BF206270.1, M34424.1, BQ711289.1, BC040431.1 and BF344248.1. On Jan 14, 2016 this sequence version replaced NM_000152.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00839.1, SRR1660809.207766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000143.2
    CDS398..3256
    Translation

    Target ORF information:

    RefSeq Version NM_000152.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000152.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079804.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073272.1
    Translation

    Target ORF information:

    RefSeq Version NM_001079804.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens glucosidase, alpha; acid (GAA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079803.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY101855.1, BC040431.1, BF206270.1, M34424.1, BQ711289.1 and BF344248.1. On Jan 14, 2016 this sequence version replaced NM_001079803.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040431.1, SRR1803611.253718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073271.1
    CDS213..3071
    Translation

    Target ORF information:

    RefSeq Version NM_001079803.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079803.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_001079804.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY101855.1, DC392486.1, BF206270.1, M34424.1, BQ711289.1, BC040431.1 and BF344248.1. On Jan 14, 2016 this sequence version replaced NM_001079804.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.5980.1, SRR1803612.6709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073272.1
    CDS133..2991
    Translation

    Target ORF information:

    RefSeq Version NM_001079804.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079804.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version NM_000152.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY101855.1, HY047261.1, HY015584.1, Y00839.1, BF206270.1, M34424.1, BQ711289.1, BC040431.1 and BF344248.1. On Nov 22, 2018 this sequence version replaced NM_000152.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00839.1, SRR1660809.207766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302262.8/ ENSP00000305692.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000143.2
    CDS343..3201
    Translation

    Target ORF information:

    RefSeq Version NM_000152.5
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000152.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version XM_005257193.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product lysosomal alpha-glucosidase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257250.1
    Translation

    Target ORF information:

    RefSeq Version XM_005257193.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version XM_005257194.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product lysosomal alpha-glucosidase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257251.1
    Translation

    Target ORF information:

    RefSeq Version XM_005257194.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257194.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version XM_005257193.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257250.1
    CDS199..3057
    Translation

    Target ORF information:

    RefSeq Version XM_005257193.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257193.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16152
    Clone ID Related Accession (Same CDS sequence) NM_000152.3 , NM_001079803.1 , NM_001079803.3 , NM_001079804.3 , NM_000152.4 , XM_005257193.1 , NM_001079804.1 , NM_001079803.2 , NM_001079804.2 , XM_005257194.3 , XM_005257193.2 , XM_005257194.4 , NM_000152.5
    Accession Version XM_005257194.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257194.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257251.1
    CDS239..3097
    Translation

    Target ORF information:

    RefSeq Version XM_005257194.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257194.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGAGTGA GGCACCCGCC CTGCTCCCAC CGGCTCCTGG CCGTCTGCGC CCTCGTGTCC 
    TTGGCAACCG CTGCACTCCT GGGGCACATC CTACTCCATG ATTTCCTGCT GGTTCCCCGA
    GAGCTGAGTG GCTCCTCCCC AGTCCTGGAG GAGACTCACC CAGCTCACCA GCAGGGAGCC
    AGCAGACCAG GGCCCCGGGA TGCCCAGGCA CACCCCGGCC GTCCCAGAGC AGTGCCCACA
    CAGTGCGACG TCCCCCCCAA CAGCCGCTTC GATTGCGCCC CTGACAAGGC CATCACCCAG
    GAACAGTGCG AGGCCCGCGG CTGTTGCTAC ATCCCTGCAA AGCAGGGGCT GCAGGGAGCC
    CAGATGGGGC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CCAGCTACAA GCTGGAGAAC
    CTGAGCTCCT CTGAAATGGG CTACACGGCC ACCCTGACCC GTACCACCCC CACCTTCTTC
    CCCAAGGACA TCCTGACCCT GCGGCTGGAC GTGATGATGG AGACTGAGAA CCGCCTCCAC
    TTCACGATCA AAGATCCAGC TAACAGGCGC TACGAGGTGC CCTTGGAGAC CCCGCATGTC
    CACAGCCGGG CACCGTCCCC ACTCTACAGC GTGGAGTTCT CCGAGGAGCC CTTCGGGGTG
    ATCGTGCGCC GGCAGCTGGA CGGCCGCGTG CTGCTGAACA CGACGGTGGC GCCCCTGTTC
    TTTGCGGACC AGTTCCTTCA GCTGTCCACC TCGCTGCCCT CGCAGTATAT CACAGGCCTC
    GCCGAGCACC TCAGTCCCCT GATGCTCAGC ACCAGCTGGA CCAGGATCAC CCTGTGGAAC
    CGGGACCTTG CGCCCACGCC CGGTGCGAAC CTCTACGGGT CTCACCCTTT CTACCTGGCG
    CTGGAGGACG GCGGGTCGGC ACACGGGGTG TTCCTGCTAA ACAGCAATGC CATGGATGTG
    GTCCTGCAGC CGAGCCCTGC CCTTAGCTGG AGGTCGACAG GTGGGATCCT GGATGTCTAC
    ATCTTCCTGG GCCCAGAGCC CAAGAGCGTG GTGCAGCAGT ACCTGGACGT TGTGGGATAC
    CCGTTCATGC CGCCATACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG CTACTCCTCC
    ACCGCTATCA CCCGCCAGGT GGTGGAGAAC ATGACCAGGG CCCACTTCCC CCTGGACGTC
    CAGTGGAACG ACCTGGACTA CATGGACTCC CGGAGGGACT TCACGTTCAA CAAGGATGGC
    TTCCGGGACT TCCCGGCCAT GGTGCAGGAG CTGCACCAGG GCGGCCGGCG CTACATGATG
    ATCGTGGATC CTGCCATCAG CAGCTCGGGC CCTGCCGGGA GCTACAGGCC CTACGACGAG
    GGTCTGCGGA GGGGGGTTTT CATCACCAAC GAGACCGGCC AGCCGCTGAT TGGGAAGGTA
    TGGCCCGGGT CCACTGCCTT CCCCGACTTC ACCAACCCCA CAGCCCTGGC CTGGTGGGAG
    GACATGGTGG CTGAGTTCCA TGACCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC
    GAGCCTTCCA ACTTCATCAG GGGCTCTGAG GACGGCTGCC CCAACAATGA GCTGGAGAAC
    CCACCCTACG TGCCTGGGGT GGTTGGGGGG ACCCTCCAGG CGGCCACCAT CTGTGCCTCC
    AGCCACCAGT TTCTCTCCAC ACACTACAAC CTGCACAACC TCTACGGCCT GACCGAAGCC
    ATCGCCTCCC ACAGGGCGCT GGTGAAGGCT CGGGGGACAC GCCCATTTGT GATCTCCCGC
    TCGACCTTTG CTGGCCACGG CCGATACGCC GGCCACTGGA CGGGGGACGT GTGGAGCTCC
    TGGGAGCAGC TCGCCTCCTC CGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGGGTGCCT
    CTGGTCGGGG CCGACGTCTG CGGCTTCCTG GGCAACACCT CAGAGGAGCT GTGTGTGCGC
    TGGACCCAGC TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAACAGCCT GCTCAGTCTG
    CCCCAGGAGC CGTACAGCTT CAGCGAGCCG GCCCAGCAGG CCATGAGGAA GGCCCTCACC
    CTGCGCTACG CACTCCTCCC CCACCTCTAC ACACTGTTCC ACCAGGCCCA CGTCGCGGGG
    GAGACCGTGG CCCGGCCCCT CTTCCTGGAG TTCCCCAAGG ACTCTAGCAC CTGGACTGTG
    GACCACCAGC TCCTGTGGGG GGAGGCCCTG CTCATCACCC CAGTGCTCCA GGCCGGGAAG
    GCCGAAGTGA CTGGCTACTT CCCCTTGGGC ACATGGTACG ACCTGCAGAC GGTGCCAGTA
    GAGGCCCTTG GCAGCCTCCC ACCCCCACCT GCAGCTCCCC GTGAGCCAGC CATCCACAGC
    GAGGGGCAGT GGGTGACGCT GCCGGCCCCC CTGGACACCA TCAACGTCCA CCTCCGGGCT
    GGGTACATCA TCCCCCTGCA GGGCCCTGGC CTCACAACCA CAGAGTCCCG CCAGCAGCCC
    ATGGCCCTGG CTGTGGCCCT GACCAAGGGT GGGGAGGCCC GAGGGGAGCT GTTCTGGGAC
    GATGGAGAGA GCCTGGAAGT GCTGGAGCGA GGGGCCTACA CACAGGTCAT CTTCCTGGCC
    AGGAATAACA CGATCGTGAA TGAGCTGGTA CGTGTGACCA GTGAGGGAGC TGGCCTGCAG
    CTGCAGAAGG TGACTGTCCT GGGCGTGGCC ACGGCGCCCC AGCAGGTCCT CTCCAACGGT
    GTCCCTGTCT CCAACTTCAC CTACAGCCCC GACACCAAGG TCCTGGACAT CTGTGTCTCG
    CTGTTGATGG GAGAGCAGTT TCTCGTCAGC TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.