ABL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABL1
Entrez Gene ID	25
Full Name	ABL proto-oncogene 1, non-receptor tyrosine kinase
Synonyms	ABL,CHDSKM,JTK7,bcr/abl,c-ABL,c-ABL1,p150,v-abl
General protein information	Preferred Names ABL proto-oncogene 1, non-receptor tyrosine kinase Names tyrosine-protein kinase ABL1 Abelson tyrosine-protein kinase 1 bcr/c-abl oncogene protein c-abl oncogene 1, receptor tyrosine kinase proto-oncogene c-Abl proto-oncogene tyrosine-protein kinase ABL1 v-abl Abelson murine leukemia viral oncogene homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.12
Summary	This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014].
Disorder MIM:	189980
Disorder Html:	Leukemia, Philadelphia chromosome-positive, resistant to imatinib (3); Congenital heart defects and skeletal malformations syndrome, 617602 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007313.2	NP_009297.2	tyrosine-protein kinase ABL1 isoform b
NM_005157.5	NP_005148.2	tyrosine-protein kinase ABL1 isoform a
NM_005157.6	NP_005148.2	tyrosine-protein kinase ABL1 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABL1	NP_009297.2
Rattus norvegicus (Norway rat)	LOC100909750	XP_003749487.1
Danio rerio (zebrafish)	abl1	XP_001337829.1
Gallus gallus (chicken)	ABL1	XP_001233812.1
Bos taurus (cattle)	ABL1	NP_001193789.1
Rattus norvegicus (Norway rat)	Abl1	NP_001094320.1
Pan troglodytes (chimpanzee)	ABL1	XP_001166213.2
Canis lupus familiaris (dog)	ABL1	XP_548413.3
Mus musculus (house mouse)	Abl1	NP_001106174.1
Xenopus tropicalis (tropical clawed frog)	abl1	XP_004916733.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypomethylation-mediated H19 overexpression increases the risk of disease evolution through the association with BCR-ABL transcript in chronic myeloid leukemia.
Zhou JD, Lin J, Zhang TJ, Ma JC, Li XX, Wen XM, Guo H, Xu ZJ, Deng ZQ, Zhang W, Qian J
Journal of cellular physiology233(3)2444-2450(2018 Mar)

Atomic view of the energy landscape in the allosteric regulation of Abl kinase.
Saleh T, Rossi P, Kalodimos CG
Nature structural & molecular biology24(11)893-901(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dehydrocostus lactone significantly inhibits the phosphorylation expression of Bcr/Abl, STAT5, JAK2, and STAT3 and downstream molecules including p-CrkL, Mcl-1, Bcl-XL, and Bcl-2 proteins in K562 cells.
Title: Dehydrocostus Lactone Suppresses Proliferation of Human Chronic Myeloid Leukemia Cells Through Bcr/Abl-JAK/STAT Signaling Pathways.

c-Abl promotes TGF-beta-induced SKIP/Smad3 interaction.
Title: Enhancement of TGF-?-induced Smad3 activity by c-Abl-mediated tyrosine phosphorylation of its coactivator SKI-interacting protein (SKIP).

We demonstrate that nanopore technology is suitable for employment in the hematology laboratory for detecting BCR-ABL1 kinase domain mutation in Philadelphia-positive leukemias.
Title: Mutational analysis in BCR-ABL1 positive leukemia by deep sequencing based on nanopore MinION technology.

the c-Abl non-receptor kinase phosphorylates DDB1 at residue Tyr-316 to recruit a small regulatory protein, DDA1, leading to increased substrate ubiquitination
Title: Activation of c-Abl Kinase Potentiates the Anti-myeloma Drug Lenalidomide by Promoting DDA1 Protein Recruitment to the CRL4 Ubiquitin Ligase.

though our data support the previous findings that co-expression of BCR-ABL transcripts is due to the occurrence of exonic and intronic polymorphisms in the BCR gene, it also shows that the intronic polymorphism can arise without the linked exonic polymorphism. The occurrence of ABL kinase domain mutation is less frequent in Indian population.
Title: Dual transcripts of

The following ABL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28559	NM_007313.2 Latest version!	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu28560	NM_005157.6 Latest version!	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
View Old Accession Versions >>
OHu28560	NM_005157.5	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABL1 ( NM_007313.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009297.2 Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu28559
Clone ID Related Accession (Same CDS sequence)	NM_007313.2
Accession Version	NM_007313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ABL1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA335983.1, AL707819.1, AB209456.1 and AA524892.1. This sequence is a reference standard in the RefSeqGene project. On Apr 7, 2005 this sequence version replaced NM_007313.1. Transcript Variant: This variant (b), which includes exon 1b but not exon 1a, represents the longer transcript and encodes the longer isoform (b). This isoform contains an N-terminal glycine which could be myristylated, and it is thus thought to be directed to the plasma membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209642.1, AB209456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC AGCCTGGAAA AGTACTTGGG GACCAAAGAA GGCCAAGCTT GCCTGCCCTG 
CATTTTATCA AAGGAGCAGG GAAGAAGGAA TCATCGAGGC ATGGGGGTCC ACACTGCAAT 
GTTTTTGTGG AACATGAAGC CCTTCAGCGG CCAGTAGCAT CTGACTTTGA GCCTCAGGGT 
CTGAGTGAAG CCGCTCGTTG GAACTCCAAG GAAAACCTTC TCGCTGGACC CAGTGAAAAT 
GACCCCAACC TTTTCGTTGC ACTGTATGAT TTTGTGGCCA GTGGAGATAA CACTCTAAGC 
ATAACTAAAG GTGAAAAGCT CCGGGTCTTA GGCTATAATC ACAATGGGGA ATGGTGTGAA 
GCCCAAACCA AAAATGGCCA AGGCTGGGTC CCAAGCAACT ACATCACGCC AGTCAACAGT 
CTGGAGAAAC ACTCCTGGTA CCATGGGCCT GTGTCCCGCA ATGCCGCTGA GTATCTGCTG 
AGCAGCGGGA TCAATGGCAG CTTCTTGGTG CGTGAGAGTG AGAGCAGTCC TGGCCAGAGG 
TCCATCTCGC TGAGATACGA AGGGAGGGTG TACCATTACA GGATCAACAC TGCTTCTGAT 
GGCAAGCTCT ACGTCTCCTC CGAGAGCCGC TTCAACACCC TGGCCGAGTT GGTTCATCAT 
CATTCAACGG TGGCCGACGG GCTCATCACC ACGCTCCATT ATCCAGCCCC AAAGCGCAAC 
AAGCCCACTG TCTATGGTGT GTCCCCCAAC TACGACAAGT GGGAGATGGA ACGCACGGAC 
ATCACCATGA AGCACAAGCT GGGCGGGGGC CAGTACGGGG AGGTGTACGA GGGCGTGTGG 
AAGAAATACA GCCTGACGGT GGCCGTGAAG ACCTTGAAGG AGGACACCAT GGAGGTGGAA 
GAGTTCTTGA AAGAAGCTGC AGTCATGAAA GAGATCAAAC ACCCTAACCT GGTGCAGCTC 
CTTGGGGTCT GCACCCGGGA GCCCCCGTTC TATATCATCA CTGAGTTCAT GACCTACGGG 
AACCTCCTGG ACTACCTGAG GGAGTGCAAC CGGCAGGAGG TGAACGCCGT GGTGCTGCTG 
TACATGGCCA CTCAGATCTC GTCAGCCATG GAGTACCTGG AGAAGAAAAA CTTCATCCAC 
AGAGATCTTG CTGCCCGAAA CTGCCTGGTA GGGGAGAACC ACTTGGTGAA GGTAGCTGAT 
TTTGGCCTGA GCAGGTTGAT GACAGGGGAC ACCTACACAG CCCATGCTGG AGCCAAGTTC 
CCCATCAAAT GGACTGCACC CGAGAGCCTG GCCTACAACA AGTTCTCCAT CAAGTCCGAC 
GTCTGGGCAT TTGGAGTATT GCTTTGGGAA ATTGCTACCT ATGGCATGTC CCCTTACCCG 
GGAATTGACC TGTCCCAGGT GTATGAGCTG CTAGAGAAGG ACTACCGCAT GGAGCGCCCA 
GAAGGCTGCC CAGAGAAGGT CTATGAACTC ATGCGAGCAT GTTGGCAGTG GAATCCCTCT 
GACCGGCCCT CCTTTGCTGA AATCCACCAA GCCTTTGAAA CAATGTTCCA GGAATCCAGT 
ATCTCAGACG AAGTGGAAAA GGAGCTGGGG AAACAAGGCG TCCGTGGGGC TGTGAGTACC 
TTGCTGCAGG CCCCAGAGCT GCCCACCAAG ACGAGGACCT CCAGGAGAGC TGCAGAGCAC 
AGAGACACCA CTGACGTGCC TGAGATGCCT CACTCCAAGG GCCAGGGAGA GAGCGATCCT 
CTGGACCATG AGCCTGCCGT GTCTCCATTG CTCCCTCGAA AAGAGCGAGG TCCCCCGGAG 
GGCGGCCTGA ATGAAGATGA GCGCCTTCTC CCCAAAGACA AAAAGACCAA CTTGTTCAGC 
GCCTTGATCA AGAAGAAGAA GAAGACAGCC CCAACCCCTC CCAAACGCAG CAGCTCCTTC 
CGGGAGATGG ACGGCCAGCC GGAGCGCAGA GGGGCCGGCG AGGAAGAGGG CCGAGACATC 
AGCAACGGGG CACTGGCTTT CACCCCCTTG GACACAGCTG ACCCAGCCAA GTCCCCAAAG 
CCCAGCAATG GGGCTGGGGT CCCCAATGGA GCCCTCCGGG AGTCCGGGGG CTCAGGCTTC 
CGGTCTCCCC ACCTGTGGAA GAAGTCCAGC ACGCTGACCA GCAGCCGCCT AGCCACCGGC 
GAGGAGGAGG GCGGTGGCAG CTCCAGCAAG CGCTTCCTGC GCTCTTGCTC CGCCTCCTGC 
GTTCCCCATG GGGCCAAGGA CACGGAGTGG AGGTCAGTCA CGCTGCCTCG GGACTTGCAG 
TCCACGGGAA GACAGTTTGA CTCGTCCACA TTTGGAGGGC ACAAAAGTGA GAAGCCGGCT 
CTGCCTCGGA AGAGGGCAGG GGAGAACAGG TCTGACCAGG TGACCCGAGG CACAGTAACG 
CCTCCCCCCA GGCTGGTGAA AAAGAATGAG GAAGCTGCTG ATGAGGTCTT CAAAGACATC 
ATGGAGTCCA GCCCGGGCTC CAGCCCGCCC AACCTGACTC CAAAACCCCT CCGGCGGCAG 
GTCACCGTGG CCCCTGCCTC GGGCCTCCCC CACAAGGAAG AAGCTGGAAA GGGCAGTGCC 
TTAGGGACCC CTGCTGCAGC TGAGCCAGTG ACCCCCACCA GCAAAGCAGG CTCAGGTGCA 
CCAGGGGGCA CCAGCAAGGG CCCCGCCGAG GAGTCCAGAG TGAGGAGGCA CAAGCACTCC 
TCTGAGTCGC CAGGGAGGGA CAAGGGGAAA TTGTCCAGGC TCAAACCTGC CCCGCCGCCC 
CCACCAGCAG CCTCTGCAGG GAAGGCTGGA GGAAAGCCCT CGCAGAGCCC GAGCCAGGAG 
GCGGCCGGGG AGGCAGTCCT GGGCGCAAAG ACAAAAGCCA CGAGTCTGGT TGATGCTGTG 
AACAGTGACG CTGCCAAGCC CAGCCAGCCG GGAGAGGGCC TCAAAAAGCC CGTGCTCCCG 
GCCACTCCAA AGCCACAGTC CGCCAAGCCG TCGGGGACCC CCATCAGCCC AGCCCCCGTT 
CCCTCCACGT TGCCATCAGC ATCCTCGGCC CTGGCAGGGG ACCAGCCGTC TTCCACCGCC 
TTCATCCCTC TCATATCAAC CCGAGTGTCT CTTCGGAAAA CCCGCCAGCC TCCAGAGCGG 
ATCGCCAGCG GCGCCATCAC CAAGGGCGTG GTCCTGGACA GCACCGAGGC GCTGTGCCTC 
GCCATCTCTA GGAACTCCGA GCAGATGGCC AGCCACAGCG CAGTGCTGGA GGCCGGCAAA 
AACCTCTACA CGTTCTGCGT GAGCTATGTG GATTCCATCC AGCAAATGAG GAACAAGTTT 
GCCTTCCGAG AGGCCATCAA CAAACTGGAG AATAATCTCC GGGAGCTTCA GATCTGCCCG 
GCGACAGCAG GCAGTGGTCC AGCGGCCACT CAGGACTTCA GCAAGCTCCT CAGTTCGGTG 
AAGGAAATCA GTGACATAGT GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009297.2
CDS	440..3889
Translation	MGQQPGKVLGDQRRPSLPALHFIKGAGKKESSRHGGPHCNVFVEHEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR

Target ORF information:

RefSeq Version	NM_007313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007313.2

ORF Insert Sequence:

ATGGGGCAGC AGCCTGGAAA AGTACTTGGG GACCAAAGAA GGCCAAGCTT GCCTGCCCTG 
CATTTTATCA AAGGAGCAGG GAAGAAGGAA TCATCGAGGC ATGGGGGTCC ACACTGCAAT 
GTTTTTGTGG AACATGAAGC CCTTCAGCGG CCAGTAGCAT CTGACTTTGA GCCTCAGGGT 
CTGAGTGAAG CCGCTCGTTG GAACTCCAAG GAAAACCTTC TCGCTGGACC CAGTGAAAAT 
GACCCCAACC TTTTCGTTGC ACTGTATGAT TTTGTGGCCA GTGGAGATAA CACTCTAAGC 
ATAACTAAAG GTGAAAAGCT CCGGGTCTTA GGCTATAATC ACAATGGGGA ATGGTGTGAA 
GCCCAAACCA AAAATGGCCA AGGCTGGGTC CCAAGCAACT ACATCACGCC AGTCAACAGT 
CTGGAGAAAC ACTCCTGGTA CCATGGGCCT GTGTCCCGCA ATGCCGCTGA GTATCTGCTG 
AGCAGCGGGA TCAATGGCAG CTTCTTGGTG CGTGAGAGTG AGAGCAGTCC TGGCCAGAGG 
TCCATCTCGC TGAGATACGA AGGGAGGGTG TACCATTACA GGATCAACAC TGCTTCTGAT 
GGCAAGCTCT ACGTCTCCTC CGAGAGCCGC TTCAACACCC TGGCCGAGTT GGTTCATCAT 
CATTCAACGG TGGCCGACGG GCTCATCACC ACGCTCCATT ATCCAGCCCC AAAGCGCAAC 
AAGCCCACTG TCTATGGTGT GTCCCCCAAC TACGACAAGT GGGAGATGGA ACGCACGGAC 
ATCACCATGA AGCACAAGCT GGGCGGGGGC CAGTACGGGG AGGTGTACGA GGGCGTGTGG 
AAGAAATACA GCCTGACGGT GGCCGTGAAG ACCTTGAAGG AGGACACCAT GGAGGTGGAA 
GAGTTCTTGA AAGAAGCTGC AGTCATGAAA GAGATCAAAC ACCCTAACCT GGTGCAGCTC 
CTTGGGGTCT GCACCCGGGA GCCCCCGTTC TATATCATCA CTGAGTTCAT GACCTACGGG 
AACCTCCTGG ACTACCTGAG GGAGTGCAAC CGGCAGGAGG TGAACGCCGT GGTGCTGCTG 
TACATGGCCA CTCAGATCTC GTCAGCCATG GAGTACCTGG AGAAGAAAAA CTTCATCCAC 
AGAGATCTTG CTGCCCGAAA CTGCCTGGTA GGGGAGAACC ACTTGGTGAA GGTAGCTGAT 
TTTGGCCTGA GCAGGTTGAT GACAGGGGAC ACCTACACAG CCCATGCTGG AGCCAAGTTC 
CCCATCAAAT GGACTGCACC CGAGAGCCTG GCCTACAACA AGTTCTCCAT CAAGTCCGAC 
GTCTGGGCAT TTGGAGTATT GCTTTGGGAA ATTGCTACCT ATGGCATGTC CCCTTACCCG 
GGAATTGACC TGTCCCAGGT GTATGAGCTG CTAGAGAAGG ACTACCGCAT GGAGCGCCCA 
GAAGGCTGCC CAGAGAAGGT CTATGAACTC ATGCGAGCAT GTTGGCAGTG GAATCCCTCT 
GACCGGCCCT CCTTTGCTGA AATCCACCAA GCCTTTGAAA CAATGTTCCA GGAATCCAGT 
ATCTCAGACG AAGTGGAAAA GGAGCTGGGG AAACAAGGCG TCCGTGGGGC TGTGAGTACC 
TTGCTGCAGG CCCCAGAGCT GCCCACCAAG ACGAGGACCT CCAGGAGAGC TGCAGAGCAC 
AGAGACACCA CTGACGTGCC TGAGATGCCT CACTCCAAGG GCCAGGGAGA GAGCGATCCT 
CTGGACCATG AGCCTGCCGT GTCTCCATTG CTCCCTCGAA AAGAGCGAGG TCCCCCGGAG 
GGCGGCCTGA ATGAAGATGA GCGCCTTCTC CCCAAAGACA AAAAGACCAA CTTGTTCAGC 
GCCTTGATCA AGAAGAAGAA GAAGACAGCC CCAACCCCTC CCAAACGCAG CAGCTCCTTC 
CGGGAGATGG ACGGCCAGCC GGAGCGCAGA GGGGCCGGCG AGGAAGAGGG CCGAGACATC 
AGCAACGGGG CACTGGCTTT CACCCCCTTG GACACAGCTG ACCCAGCCAA GTCCCCAAAG 
CCCAGCAATG GGGCTGGGGT CCCCAATGGA GCCCTCCGGG AGTCCGGGGG CTCAGGCTTC 
CGGTCTCCCC ACCTGTGGAA GAAGTCCAGC ACGCTGACCA GCAGCCGCCT AGCCACCGGC 
GAGGAGGAGG GCGGTGGCAG CTCCAGCAAG CGCTTCCTGC GCTCTTGCTC CGCCTCCTGC 
GTTCCCCATG GGGCCAAGGA CACGGAGTGG AGGTCAGTCA CGCTGCCTCG GGACTTGCAG 
TCCACGGGAA GACAGTTTGA CTCGTCCACA TTTGGAGGGC ACAAAAGTGA GAAGCCGGCT 
CTGCCTCGGA AGAGGGCAGG GGAGAACAGG TCTGACCAGG TGACCCGAGG CACAGTAACG 
CCTCCCCCCA GGCTGGTGAA AAAGAATGAG GAAGCTGCTG ATGAGGTCTT CAAAGACATC 
ATGGAGTCCA GCCCGGGCTC CAGCCCGCCC AACCTGACTC CAAAACCCCT CCGGCGGCAG 
GTCACCGTGG CCCCTGCCTC GGGCCTCCCC CACAAGGAAG AAGCTGGAAA GGGCAGTGCC 
TTAGGGACCC CTGCTGCAGC TGAGCCAGTG ACCCCCACCA GCAAAGCAGG CTCAGGTGCA 
CCAGGGGGCA CCAGCAAGGG CCCCGCCGAG GAGTCCAGAG TGAGGAGGCA CAAGCACTCC 
TCTGAGTCGC CAGGGAGGGA CAAGGGGAAA TTGTCCAGGC TCAAACCTGC CCCGCCGCCC 
CCACCAGCAG CCTCTGCAGG GAAGGCTGGA GGAAAGCCCT CGCAGAGCCC GAGCCAGGAG 
GCGGCCGGGG AGGCAGTCCT GGGCGCAAAG ACAAAAGCCA CGAGTCTGGT TGATGCTGTG 
AACAGTGACG CTGCCAAGCC CAGCCAGCCG GGAGAGGGCC TCAAAAAGCC CGTGCTCCCG 
GCCACTCCAA AGCCACAGTC CGCCAAGCCG TCGGGGACCC CCATCAGCCC AGCCCCCGTT 
CCCTCCACGT TGCCATCAGC ATCCTCGGCC CTGGCAGGGG ACCAGCCGTC TTCCACCGCC 
TTCATCCCTC TCATATCAAC CCGAGTGTCT CTTCGGAAAA CCCGCCAGCC TCCAGAGCGG 
ATCGCCAGCG GCGCCATCAC CAAGGGCGTG GTCCTGGACA GCACCGAGGC GCTGTGCCTC 
GCCATCTCTA GGAACTCCGA GCAGATGGCC AGCCACAGCG CAGTGCTGGA GGCCGGCAAA 
AACCTCTACA CGTTCTGCGT GAGCTATGTG GATTCCATCC AGCAAATGAG GAACAAGTTT 
GCCTTCCGAG AGGCCATCAA CAAACTGGAG AATAATCTCC GGGAGCTTCA GATCTGCCCG 
GCGACAGCAG GCAGTGGTCC AGCGGCCACT CAGGACTTCA GCAAGCTCCT CAGTTCGGTG 
AAGGAAATCA GTGACATAGT GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABL1 ( NM_005157.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005148.2 Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.

Price & Availability↑

CloneID	OHu28560
Clone ID Related Accession (Same CDS sequence)	NM_005157.5 , NM_005157.6
Accession Version	NM_005157.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ABL1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M14752.1, AL161733.20, X16416.1, AB209456.1 and AI458416.1. This sequence is a reference standard in the RefSeqGene project. On Aug 6, 2014 this sequence version replaced NM_005157.4. Transcript Variant: This variant (a) includes an alternate 5' terminal exon (exon 1a instead of exon 1b), and it thus differs in the 5' UTR and 5' coding region, compared to variant b. The encoded isoform (a), which is localized in the nucleus, has a distinct N-terminus and is shorter than isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16416.1, SRR1660805.38625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTGGAGA TCTGCCTGAA GCTGGTGGGC TGCAAATCCA AGAAGGGGCT GTCCTCGTCC 
TCCAGCTGTT ATCTGGAAGA AGCCCTTCAG CGGCCAGTAG CATCTGACTT TGAGCCTCAG 
GGTCTGAGTG AAGCCGCTCG TTGGAACTCC AAGGAAAACC TTCTCGCTGG ACCCAGTGAA 
AATGACCCCA ACCTTTTCGT TGCACTGTAT GATTTTGTGG CCAGTGGAGA TAACACTCTA 
AGCATAACTA AAGGTGAAAA GCTCCGGGTC TTAGGCTATA ATCACAATGG GGAATGGTGT 
GAAGCCCAAA CCAAAAATGG CCAAGGCTGG GTCCCAAGCA ACTACATCAC GCCAGTCAAC 
AGTCTGGAGA AACACTCCTG GTACCATGGG CCTGTGTCCC GCAATGCCGC TGAGTATCTG 
CTGAGCAGCG GGATCAATGG CAGCTTCTTG GTGCGTGAGA GTGAGAGCAG TCCTGGCCAG 
AGGTCCATCT CGCTGAGATA CGAAGGGAGG GTGTACCATT ACAGGATCAA CACTGCTTCT 
GATGGCAAGC TCTACGTCTC CTCCGAGAGC CGCTTCAACA CCCTGGCCGA GTTGGTTCAT 
CATCATTCAA CGGTGGCCGA CGGGCTCATC ACCACGCTCC ATTATCCAGC CCCAAAGCGC 
AACAAGCCCA CTGTCTATGG TGTGTCCCCC AACTACGACA AGTGGGAGAT GGAACGCACG 
GACATCACCA TGAAGCACAA GCTGGGCGGG GGCCAGTACG GGGAGGTGTA CGAGGGCGTG 
TGGAAGAAAT ACAGCCTGAC GGTGGCCGTG AAGACCTTGA AGGAGGACAC CATGGAGGTG 
GAAGAGTTCT TGAAAGAAGC TGCAGTCATG AAAGAGATCA AACACCCTAA CCTGGTGCAG 
CTCCTTGGGG TCTGCACCCG GGAGCCCCCG TTCTATATCA TCACTGAGTT CATGACCTAC 
GGGAACCTCC TGGACTACCT GAGGGAGTGC AACCGGCAGG AGGTGAACGC CGTGGTGCTG 
CTGTACATGG CCACTCAGAT CTCGTCAGCC ATGGAGTACC TGGAGAAGAA AAACTTCATC 
CACAGAGATC TTGCTGCCCG AAACTGCCTG GTAGGGGAGA ACCACTTGGT GAAGGTAGCT 
GATTTTGGCC TGAGCAGGTT GATGACAGGG GACACCTACA CAGCCCATGC TGGAGCCAAG 
TTCCCCATCA AATGGACTGC ACCCGAGAGC CTGGCCTACA ACAAGTTCTC CATCAAGTCC 
GACGTCTGGG CATTTGGAGT ATTGCTTTGG GAAATTGCTA CCTATGGCAT GTCCCCTTAC 
CCGGGAATTG ACCTGTCCCA GGTGTATGAG CTGCTAGAGA AGGACTACCG CATGGAGCGC 
CCAGAAGGCT GCCCAGAGAA GGTCTATGAA CTCATGCGAG CATGTTGGCA GTGGAATCCC 
TCTGACCGGC CCTCCTTTGC TGAAATCCAC CAAGCCTTTG AAACAATGTT CCAGGAATCC 
AGTATCTCAG ACGAAGTGGA AAAGGAGCTG GGGAAACAAG GCGTCCGTGG GGCTGTGAGT 
ACCTTGCTGC AGGCCCCAGA GCTGCCCACC AAGACGAGGA CCTCCAGGAG AGCTGCAGAG 
CACAGAGACA CCACTGACGT GCCTGAGATG CCTCACTCCA AGGGCCAGGG AGAGAGCGAT 
CCTCTGGACC ATGAGCCTGC CGTGTCTCCA TTGCTCCCTC GAAAAGAGCG AGGTCCCCCG 
GAGGGCGGCC TGAATGAAGA TGAGCGCCTT CTCCCCAAAG ACAAAAAGAC CAACTTGTTC 
AGCGCCTTGA TCAAGAAGAA GAAGAAGACA GCCCCAACCC CTCCCAAACG CAGCAGCTCC 
TTCCGGGAGA TGGACGGCCA GCCGGAGCGC AGAGGGGCCG GCGAGGAAGA GGGCCGAGAC 
ATCAGCAACG GGGCACTGGC TTTCACCCCC TTGGACACAG CTGACCCAGC CAAGTCCCCA 
AAGCCCAGCA ATGGGGCTGG GGTCCCCAAT GGAGCCCTCC GGGAGTCCGG GGGCTCAGGC 
TTCCGGTCTC CCCACCTGTG GAAGAAGTCC AGCACGCTGA CCAGCAGCCG CCTAGCCACC 
GGCGAGGAGG AGGGCGGTGG CAGCTCCAGC AAGCGCTTCC TGCGCTCTTG CTCCGCCTCC 
TGCGTTCCCC ATGGGGCCAA GGACACGGAG TGGAGGTCAG TCACGCTGCC TCGGGACTTG 
CAGTCCACGG GAAGACAGTT TGACTCGTCC ACATTTGGAG GGCACAAAAG TGAGAAGCCG 
GCTCTGCCTC GGAAGAGGGC AGGGGAGAAC AGGTCTGACC AGGTGACCCG AGGCACAGTA 
ACGCCTCCCC CCAGGCTGGT GAAAAAGAAT GAGGAAGCTG CTGATGAGGT CTTCAAAGAC 
ATCATGGAGT CCAGCCCGGG CTCCAGCCCG CCCAACCTGA CTCCAAAACC CCTCCGGCGG 
CAGGTCACCG TGGCCCCTGC CTCGGGCCTC CCCCACAAGG AAGAAGCTGG AAAGGGCAGT 
GCCTTAGGGA CCCCTGCTGC AGCTGAGCCA GTGACCCCCA CCAGCAAAGC AGGCTCAGGT 
GCACCAGGGG GCACCAGCAA GGGCCCCGCC GAGGAGTCCA GAGTGAGGAG GCACAAGCAC 
TCCTCTGAGT CGCCAGGGAG GGACAAGGGG AAATTGTCCA GGCTCAAACC TGCCCCGCCG 
CCCCCACCAG CAGCCTCTGC AGGGAAGGCT GGAGGAAAGC CCTCGCAGAG CCCGAGCCAG 
GAGGCGGCCG GGGAGGCAGT CCTGGGCGCA AAGACAAAAG CCACGAGTCT GGTTGATGCT 
GTGAACAGTG ACGCTGCCAA GCCCAGCCAG CCGGGAGAGG GCCTCAAAAA GCCCGTGCTC 
CCGGCCACTC CAAAGCCACA GTCCGCCAAG CCGTCGGGGA CCCCCATCAG CCCAGCCCCC 
GTTCCCTCCA CGTTGCCATC AGCATCCTCG GCCCTGGCAG GGGACCAGCC GTCTTCCACC 
GCCTTCATCC CTCTCATATC AACCCGAGTG TCTCTTCGGA AAACCCGCCA GCCTCCAGAG 
CGGATCGCCA GCGGCGCCAT CACCAAGGGC GTGGTCCTGG ACAGCACCGA GGCGCTGTGC 
CTCGCCATCT CTAGGAACTC CGAGCAGATG GCCAGCCACA GCGCAGTGCT GGAGGCCGGC 
AAAAACCTCT ACACGTTCTG CGTGAGCTAT GTGGATTCCA TCCAGCAAAT GAGGAACAAG 
TTTGCCTTCC GAGAGGCCAT CAACAAACTG GAGAATAATC TCCGGGAGCT TCAGATCTGC 
CCGGCGACAG CAGGCAGTGG TCCAGCGGCC ACTCAGGACT TCAGCAAGCT CCTCAGTTCG 
GTGAAGGAAA TCAGTGACAT AGTGCAGAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005148.2
CDS	193..3585
Translation	MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR

Target ORF information:

RefSeq Version	NM_005157.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005157.5

ORF Insert Sequence:

ATGTTGGAGA TCTGCCTGAA GCTGGTGGGC TGCAAATCCA AGAAGGGGCT GTCCTCGTCC 
TCCAGCTGTT ATCTGGAAGA AGCCCTTCAG CGGCCAGTAG CATCTGACTT TGAGCCTCAG 
GGTCTGAGTG AAGCCGCTCG TTGGAACTCC AAGGAAAACC TTCTCGCTGG ACCCAGTGAA 
AATGACCCCA ACCTTTTCGT TGCACTGTAT GATTTTGTGG CCAGTGGAGA TAACACTCTA 
AGCATAACTA AAGGTGAAAA GCTCCGGGTC TTAGGCTATA ATCACAATGG GGAATGGTGT 
GAAGCCCAAA CCAAAAATGG CCAAGGCTGG GTCCCAAGCA ACTACATCAC GCCAGTCAAC 
AGTCTGGAGA AACACTCCTG GTACCATGGG CCTGTGTCCC GCAATGCCGC TGAGTATCTG 
CTGAGCAGCG GGATCAATGG CAGCTTCTTG GTGCGTGAGA GTGAGAGCAG TCCTGGCCAG 
AGGTCCATCT CGCTGAGATA CGAAGGGAGG GTGTACCATT ACAGGATCAA CACTGCTTCT 
GATGGCAAGC TCTACGTCTC CTCCGAGAGC CGCTTCAACA CCCTGGCCGA GTTGGTTCAT 
CATCATTCAA CGGTGGCCGA CGGGCTCATC ACCACGCTCC ATTATCCAGC CCCAAAGCGC 
AACAAGCCCA CTGTCTATGG TGTGTCCCCC AACTACGACA AGTGGGAGAT GGAACGCACG 
GACATCACCA TGAAGCACAA GCTGGGCGGG GGCCAGTACG GGGAGGTGTA CGAGGGCGTG 
TGGAAGAAAT ACAGCCTGAC GGTGGCCGTG AAGACCTTGA AGGAGGACAC CATGGAGGTG 
GAAGAGTTCT TGAAAGAAGC TGCAGTCATG AAAGAGATCA AACACCCTAA CCTGGTGCAG 
CTCCTTGGGG TCTGCACCCG GGAGCCCCCG TTCTATATCA TCACTGAGTT CATGACCTAC 
GGGAACCTCC TGGACTACCT GAGGGAGTGC AACCGGCAGG AGGTGAACGC CGTGGTGCTG 
CTGTACATGG CCACTCAGAT CTCGTCAGCC ATGGAGTACC TGGAGAAGAA AAACTTCATC 
CACAGAGATC TTGCTGCCCG AAACTGCCTG GTAGGGGAGA ACCACTTGGT GAAGGTAGCT 
GATTTTGGCC TGAGCAGGTT GATGACAGGG GACACCTACA CAGCCCATGC TGGAGCCAAG 
TTCCCCATCA AATGGACTGC ACCCGAGAGC CTGGCCTACA ACAAGTTCTC CATCAAGTCC 
GACGTCTGGG CATTTGGAGT ATTGCTTTGG GAAATTGCTA CCTATGGCAT GTCCCCTTAC 
CCGGGAATTG ACCTGTCCCA GGTGTATGAG CTGCTAGAGA AGGACTACCG CATGGAGCGC 
CCAGAAGGCT GCCCAGAGAA GGTCTATGAA CTCATGCGAG CATGTTGGCA GTGGAATCCC 
TCTGACCGGC CCTCCTTTGC TGAAATCCAC CAAGCCTTTG AAACAATGTT CCAGGAATCC 
AGTATCTCAG ACGAAGTGGA AAAGGAGCTG GGGAAACAAG GCGTCCGTGG GGCTGTGAGT 
ACCTTGCTGC AGGCCCCAGA GCTGCCCACC AAGACGAGGA CCTCCAGGAG AGCTGCAGAG 
CACAGAGACA CCACTGACGT GCCTGAGATG CCTCACTCCA AGGGCCAGGG AGAGAGCGAT 
CCTCTGGACC ATGAGCCTGC CGTGTCTCCA TTGCTCCCTC GAAAAGAGCG AGGTCCCCCG 
GAGGGCGGCC TGAATGAAGA TGAGCGCCTT CTCCCCAAAG ACAAAAAGAC CAACTTGTTC 
AGCGCCTTGA TCAAGAAGAA GAAGAAGACA GCCCCAACCC CTCCCAAACG CAGCAGCTCC 
TTCCGGGAGA TGGACGGCCA GCCGGAGCGC AGAGGGGCCG GCGAGGAAGA GGGCCGAGAC 
ATCAGCAACG GGGCACTGGC TTTCACCCCC TTGGACACAG CTGACCCAGC CAAGTCCCCA 
AAGCCCAGCA ATGGGGCTGG GGTCCCCAAT GGAGCCCTCC GGGAGTCCGG GGGCTCAGGC 
TTCCGGTCTC CCCACCTGTG GAAGAAGTCC AGCACGCTGA CCAGCAGCCG CCTAGCCACC 
GGCGAGGAGG AGGGCGGTGG CAGCTCCAGC AAGCGCTTCC TGCGCTCTTG CTCCGCCTCC 
TGCGTTCCCC ATGGGGCCAA GGACACGGAG TGGAGGTCAG TCACGCTGCC TCGGGACTTG 
CAGTCCACGG GAAGACAGTT TGACTCGTCC ACATTTGGAG GGCACAAAAG TGAGAAGCCG 
GCTCTGCCTC GGAAGAGGGC AGGGGAGAAC AGGTCTGACC AGGTGACCCG AGGCACAGTA 
ACGCCTCCCC CCAGGCTGGT GAAAAAGAAT GAGGAAGCTG CTGATGAGGT CTTCAAAGAC 
ATCATGGAGT CCAGCCCGGG CTCCAGCCCG CCCAACCTGA CTCCAAAACC CCTCCGGCGG 
CAGGTCACCG TGGCCCCTGC CTCGGGCCTC CCCCACAAGG AAGAAGCTGG AAAGGGCAGT 
GCCTTAGGGA CCCCTGCTGC AGCTGAGCCA GTGACCCCCA CCAGCAAAGC AGGCTCAGGT 
GCACCAGGGG GCACCAGCAA GGGCCCCGCC GAGGAGTCCA GAGTGAGGAG GCACAAGCAC 
TCCTCTGAGT CGCCAGGGAG GGACAAGGGG AAATTGTCCA GGCTCAAACC TGCCCCGCCG 
CCCCCACCAG CAGCCTCTGC AGGGAAGGCT GGAGGAAAGC CCTCGCAGAG CCCGAGCCAG 
GAGGCGGCCG GGGAGGCAGT CCTGGGCGCA AAGACAAAAG CCACGAGTCT GGTTGATGCT 
GTGAACAGTG ACGCTGCCAA GCCCAGCCAG CCGGGAGAGG GCCTCAAAAA GCCCGTGCTC 
CCGGCCACTC CAAAGCCACA GTCCGCCAAG CCGTCGGGGA CCCCCATCAG CCCAGCCCCC 
GTTCCCTCCA CGTTGCCATC AGCATCCTCG GCCCTGGCAG GGGACCAGCC GTCTTCCACC 
GCCTTCATCC CTCTCATATC AACCCGAGTG TCTCTTCGGA AAACCCGCCA GCCTCCAGAG 
CGGATCGCCA GCGGCGCCAT CACCAAGGGC GTGGTCCTGG ACAGCACCGA GGCGCTGTGC 
CTCGCCATCT CTAGGAACTC CGAGCAGATG GCCAGCCACA GCGCAGTGCT GGAGGCCGGC 
AAAAACCTCT ACACGTTCTG CGTGAGCTAT GTGGATTCCA TCCAGCAAAT GAGGAACAAG 
TTTGCCTTCC GAGAGGCCAT CAACAAACTG GAGAATAATC TCCGGGAGCT TCAGATCTGC 
CCGGCGACAG CAGGCAGTGG TCCAGCGGCC ACTCAGGACT TCAGCAAGCT CCTCAGTTCG 
GTGAAGGAAA TCAGTGACAT AGTGCAGAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABL1 ( NM_005157.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005148.2 Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.

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CloneID	OHu28560
Clone ID Related Accession (Same CDS sequence)	NM_005157.5 , NM_005157.6
Accession Version	NM_005157.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ABL1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161733.20, M14752.1, X16416.1, AB209456.1 and AI458416.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005157.5. Transcript Variant: This variant (a) includes an alternate 5' terminal exon (exon 1a instead of exon 1b), and it thus differs in the 5' UTR and 5' coding region, compared to variant b. The encoded isoform (a), which is localized in the nucleus, has a distinct N-terminus and is shorter than isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16416.1, SRR1660805.38625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000318560.6/ ENSP00000323315.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGAGA TCTGCCTGAA GCTGGTGGGC TGCAAATCCA AGAAGGGGCT GTCCTCGTCC 
TCCAGCTGTT ATCTGGAAGA AGCCCTTCAG CGGCCAGTAG CATCTGACTT TGAGCCTCAG 
GGTCTGAGTG AAGCCGCTCG TTGGAACTCC AAGGAAAACC TTCTCGCTGG ACCCAGTGAA 
AATGACCCCA ACCTTTTCGT TGCACTGTAT GATTTTGTGG CCAGTGGAGA TAACACTCTA 
AGCATAACTA AAGGTGAAAA GCTCCGGGTC TTAGGCTATA ATCACAATGG GGAATGGTGT 
GAAGCCCAAA CCAAAAATGG CCAAGGCTGG GTCCCAAGCA ACTACATCAC GCCAGTCAAC 
AGTCTGGAGA AACACTCCTG GTACCATGGG CCTGTGTCCC GCAATGCCGC TGAGTATCTG 
CTGAGCAGCG GGATCAATGG CAGCTTCTTG GTGCGTGAGA GTGAGAGCAG TCCTGGCCAG 
AGGTCCATCT CGCTGAGATA CGAAGGGAGG GTGTACCATT ACAGGATCAA CACTGCTTCT 
GATGGCAAGC TCTACGTCTC CTCCGAGAGC CGCTTCAACA CCCTGGCCGA GTTGGTTCAT 
CATCATTCAA CGGTGGCCGA CGGGCTCATC ACCACGCTCC ATTATCCAGC CCCAAAGCGC 
AACAAGCCCA CTGTCTATGG TGTGTCCCCC AACTACGACA AGTGGGAGAT GGAACGCACG 
GACATCACCA TGAAGCACAA GCTGGGCGGG GGCCAGTACG GGGAGGTGTA CGAGGGCGTG 
TGGAAGAAAT ACAGCCTGAC GGTGGCCGTG AAGACCTTGA AGGAGGACAC CATGGAGGTG 
GAAGAGTTCT TGAAAGAAGC TGCAGTCATG AAAGAGATCA AACACCCTAA CCTGGTGCAG 
CTCCTTGGGG TCTGCACCCG GGAGCCCCCG TTCTATATCA TCACTGAGTT CATGACCTAC 
GGGAACCTCC TGGACTACCT GAGGGAGTGC AACCGGCAGG AGGTGAACGC CGTGGTGCTG 
CTGTACATGG CCACTCAGAT CTCGTCAGCC ATGGAGTACC TGGAGAAGAA AAACTTCATC 
CACAGAGATC TTGCTGCCCG AAACTGCCTG GTAGGGGAGA ACCACTTGGT GAAGGTAGCT 
GATTTTGGCC TGAGCAGGTT GATGACAGGG GACACCTACA CAGCCCATGC TGGAGCCAAG 
TTCCCCATCA AATGGACTGC ACCCGAGAGC CTGGCCTACA ACAAGTTCTC CATCAAGTCC 
GACGTCTGGG CATTTGGAGT ATTGCTTTGG GAAATTGCTA CCTATGGCAT GTCCCCTTAC 
CCGGGAATTG ACCTGTCCCA GGTGTATGAG CTGCTAGAGA AGGACTACCG CATGGAGCGC 
CCAGAAGGCT GCCCAGAGAA GGTCTATGAA CTCATGCGAG CATGTTGGCA GTGGAATCCC 
TCTGACCGGC CCTCCTTTGC TGAAATCCAC CAAGCCTTTG AAACAATGTT CCAGGAATCC 
AGTATCTCAG ACGAAGTGGA AAAGGAGCTG GGGAAACAAG GCGTCCGTGG GGCTGTGAGT 
ACCTTGCTGC AGGCCCCAGA GCTGCCCACC AAGACGAGGA CCTCCAGGAG AGCTGCAGAG 
CACAGAGACA CCACTGACGT GCCTGAGATG CCTCACTCCA AGGGCCAGGG AGAGAGCGAT 
CCTCTGGACC ATGAGCCTGC CGTGTCTCCA TTGCTCCCTC GAAAAGAGCG AGGTCCCCCG 
GAGGGCGGCC TGAATGAAGA TGAGCGCCTT CTCCCCAAAG ACAAAAAGAC CAACTTGTTC 
AGCGCCTTGA TCAAGAAGAA GAAGAAGACA GCCCCAACCC CTCCCAAACG CAGCAGCTCC 
TTCCGGGAGA TGGACGGCCA GCCGGAGCGC AGAGGGGCCG GCGAGGAAGA GGGCCGAGAC 
ATCAGCAACG GGGCACTGGC TTTCACCCCC TTGGACACAG CTGACCCAGC CAAGTCCCCA 
AAGCCCAGCA ATGGGGCTGG GGTCCCCAAT GGAGCCCTCC GGGAGTCCGG GGGCTCAGGC 
TTCCGGTCTC CCCACCTGTG GAAGAAGTCC AGCACGCTGA CCAGCAGCCG CCTAGCCACC 
GGCGAGGAGG AGGGCGGTGG CAGCTCCAGC AAGCGCTTCC TGCGCTCTTG CTCCGCCTCC 
TGCGTTCCCC ATGGGGCCAA GGACACGGAG TGGAGGTCAG TCACGCTGCC TCGGGACTTG 
CAGTCCACGG GAAGACAGTT TGACTCGTCC ACATTTGGAG GGCACAAAAG TGAGAAGCCG 
GCTCTGCCTC GGAAGAGGGC AGGGGAGAAC AGGTCTGACC AGGTGACCCG AGGCACAGTA 
ACGCCTCCCC CCAGGCTGGT GAAAAAGAAT GAGGAAGCTG CTGATGAGGT CTTCAAAGAC 
ATCATGGAGT CCAGCCCGGG CTCCAGCCCG CCCAACCTGA CTCCAAAACC CCTCCGGCGG 
CAGGTCACCG TGGCCCCTGC CTCGGGCCTC CCCCACAAGG AAGAAGCTGG AAAGGGCAGT 
GCCTTAGGGA CCCCTGCTGC AGCTGAGCCA GTGACCCCCA CCAGCAAAGC AGGCTCAGGT 
GCACCAGGGG GCACCAGCAA GGGCCCCGCC GAGGAGTCCA GAGTGAGGAG GCACAAGCAC 
TCCTCTGAGT CGCCAGGGAG GGACAAGGGG AAATTGTCCA GGCTCAAACC TGCCCCGCCG 
CCCCCACCAG CAGCCTCTGC AGGGAAGGCT GGAGGAAAGC CCTCGCAGAG CCCGAGCCAG 
GAGGCGGCCG GGGAGGCAGT CCTGGGCGCA AAGACAAAAG CCACGAGTCT GGTTGATGCT 
GTGAACAGTG ACGCTGCCAA GCCCAGCCAG CCGGGAGAGG GCCTCAAAAA GCCCGTGCTC 
CCGGCCACTC CAAAGCCACA GTCCGCCAAG CCGTCGGGGA CCCCCATCAG CCCAGCCCCC 
GTTCCCTCCA CGTTGCCATC AGCATCCTCG GCCCTGGCAG GGGACCAGCC GTCTTCCACC 
GCCTTCATCC CTCTCATATC AACCCGAGTG TCTCTTCGGA AAACCCGCCA GCCTCCAGAG 
CGGATCGCCA GCGGCGCCAT CACCAAGGGC GTGGTCCTGG ACAGCACCGA GGCGCTGTGC 
CTCGCCATCT CTAGGAACTC CGAGCAGATG GCCAGCCACA GCGCAGTGCT GGAGGCCGGC 
AAAAACCTCT ACACGTTCTG CGTGAGCTAT GTGGATTCCA TCCAGCAAAT GAGGAACAAG 
TTTGCCTTCC GAGAGGCCAT CAACAAACTG GAGAATAATC TCCGGGAGCT TCAGATCTGC 
CCGGCGACAG CAGGCAGTGG TCCAGCGGCC ACTCAGGACT TCAGCAAGCT CCTCAGTTCG 
GTGAAGGAAA TCAGTGACAT AGTGCAGAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005148.2
CDS	194..3586
Translation	MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR

Target ORF information:

RefSeq Version	NM_005157.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005157.6

ORF Insert Sequence:

ATGTTGGAGA TCTGCCTGAA GCTGGTGGGC TGCAAATCCA AGAAGGGGCT GTCCTCGTCC 
TCCAGCTGTT ATCTGGAAGA AGCCCTTCAG CGGCCAGTAG CATCTGACTT TGAGCCTCAG 
GGTCTGAGTG AAGCCGCTCG TTGGAACTCC AAGGAAAACC TTCTCGCTGG ACCCAGTGAA 
AATGACCCCA ACCTTTTCGT TGCACTGTAT GATTTTGTGG CCAGTGGAGA TAACACTCTA 
AGCATAACTA AAGGTGAAAA GCTCCGGGTC TTAGGCTATA ATCACAATGG GGAATGGTGT 
GAAGCCCAAA CCAAAAATGG CCAAGGCTGG GTCCCAAGCA ACTACATCAC GCCAGTCAAC 
AGTCTGGAGA AACACTCCTG GTACCATGGG CCTGTGTCCC GCAATGCCGC TGAGTATCTG 
CTGAGCAGCG GGATCAATGG CAGCTTCTTG GTGCGTGAGA GTGAGAGCAG TCCTGGCCAG 
AGGTCCATCT CGCTGAGATA CGAAGGGAGG GTGTACCATT ACAGGATCAA CACTGCTTCT 
GATGGCAAGC TCTACGTCTC CTCCGAGAGC CGCTTCAACA CCCTGGCCGA GTTGGTTCAT 
CATCATTCAA CGGTGGCCGA CGGGCTCATC ACCACGCTCC ATTATCCAGC CCCAAAGCGC 
AACAAGCCCA CTGTCTATGG TGTGTCCCCC AACTACGACA AGTGGGAGAT GGAACGCACG 
GACATCACCA TGAAGCACAA GCTGGGCGGG GGCCAGTACG GGGAGGTGTA CGAGGGCGTG 
TGGAAGAAAT ACAGCCTGAC GGTGGCCGTG AAGACCTTGA AGGAGGACAC CATGGAGGTG 
GAAGAGTTCT TGAAAGAAGC TGCAGTCATG AAAGAGATCA AACACCCTAA CCTGGTGCAG 
CTCCTTGGGG TCTGCACCCG GGAGCCCCCG TTCTATATCA TCACTGAGTT CATGACCTAC 
GGGAACCTCC TGGACTACCT GAGGGAGTGC AACCGGCAGG AGGTGAACGC CGTGGTGCTG 
CTGTACATGG CCACTCAGAT CTCGTCAGCC ATGGAGTACC TGGAGAAGAA AAACTTCATC 
CACAGAGATC TTGCTGCCCG AAACTGCCTG GTAGGGGAGA ACCACTTGGT GAAGGTAGCT 
GATTTTGGCC TGAGCAGGTT GATGACAGGG GACACCTACA CAGCCCATGC TGGAGCCAAG 
TTCCCCATCA AATGGACTGC ACCCGAGAGC CTGGCCTACA ACAAGTTCTC CATCAAGTCC 
GACGTCTGGG CATTTGGAGT ATTGCTTTGG GAAATTGCTA CCTATGGCAT GTCCCCTTAC 
CCGGGAATTG ACCTGTCCCA GGTGTATGAG CTGCTAGAGA AGGACTACCG CATGGAGCGC 
CCAGAAGGCT GCCCAGAGAA GGTCTATGAA CTCATGCGAG CATGTTGGCA GTGGAATCCC 
TCTGACCGGC CCTCCTTTGC TGAAATCCAC CAAGCCTTTG AAACAATGTT CCAGGAATCC 
AGTATCTCAG ACGAAGTGGA AAAGGAGCTG GGGAAACAAG GCGTCCGTGG GGCTGTGAGT 
ACCTTGCTGC AGGCCCCAGA GCTGCCCACC AAGACGAGGA CCTCCAGGAG AGCTGCAGAG 
CACAGAGACA CCACTGACGT GCCTGAGATG CCTCACTCCA AGGGCCAGGG AGAGAGCGAT 
CCTCTGGACC ATGAGCCTGC CGTGTCTCCA TTGCTCCCTC GAAAAGAGCG AGGTCCCCCG 
GAGGGCGGCC TGAATGAAGA TGAGCGCCTT CTCCCCAAAG ACAAAAAGAC CAACTTGTTC 
AGCGCCTTGA TCAAGAAGAA GAAGAAGACA GCCCCAACCC CTCCCAAACG CAGCAGCTCC 
TTCCGGGAGA TGGACGGCCA GCCGGAGCGC AGAGGGGCCG GCGAGGAAGA GGGCCGAGAC 
ATCAGCAACG GGGCACTGGC TTTCACCCCC TTGGACACAG CTGACCCAGC CAAGTCCCCA 
AAGCCCAGCA ATGGGGCTGG GGTCCCCAAT GGAGCCCTCC GGGAGTCCGG GGGCTCAGGC 
TTCCGGTCTC CCCACCTGTG GAAGAAGTCC AGCACGCTGA CCAGCAGCCG CCTAGCCACC 
GGCGAGGAGG AGGGCGGTGG CAGCTCCAGC AAGCGCTTCC TGCGCTCTTG CTCCGCCTCC 
TGCGTTCCCC ATGGGGCCAA GGACACGGAG TGGAGGTCAG TCACGCTGCC TCGGGACTTG 
CAGTCCACGG GAAGACAGTT TGACTCGTCC ACATTTGGAG GGCACAAAAG TGAGAAGCCG 
GCTCTGCCTC GGAAGAGGGC AGGGGAGAAC AGGTCTGACC AGGTGACCCG AGGCACAGTA 
ACGCCTCCCC CCAGGCTGGT GAAAAAGAAT GAGGAAGCTG CTGATGAGGT CTTCAAAGAC 
ATCATGGAGT CCAGCCCGGG CTCCAGCCCG CCCAACCTGA CTCCAAAACC CCTCCGGCGG 
CAGGTCACCG TGGCCCCTGC CTCGGGCCTC CCCCACAAGG AAGAAGCTGG AAAGGGCAGT 
GCCTTAGGGA CCCCTGCTGC AGCTGAGCCA GTGACCCCCA CCAGCAAAGC AGGCTCAGGT 
GCACCAGGGG GCACCAGCAA GGGCCCCGCC GAGGAGTCCA GAGTGAGGAG GCACAAGCAC 
TCCTCTGAGT CGCCAGGGAG GGACAAGGGG AAATTGTCCA GGCTCAAACC TGCCCCGCCG 
CCCCCACCAG CAGCCTCTGC AGGGAAGGCT GGAGGAAAGC CCTCGCAGAG CCCGAGCCAG 
GAGGCGGCCG GGGAGGCAGT CCTGGGCGCA AAGACAAAAG CCACGAGTCT GGTTGATGCT 
GTGAACAGTG ACGCTGCCAA GCCCAGCCAG CCGGGAGAGG GCCTCAAAAA GCCCGTGCTC 
CCGGCCACTC CAAAGCCACA GTCCGCCAAG CCGTCGGGGA CCCCCATCAG CCCAGCCCCC 
GTTCCCTCCA CGTTGCCATC AGCATCCTCG GCCCTGGCAG GGGACCAGCC GTCTTCCACC 
GCCTTCATCC CTCTCATATC AACCCGAGTG TCTCTTCGGA AAACCCGCCA GCCTCCAGAG 
CGGATCGCCA GCGGCGCCAT CACCAAGGGC GTGGTCCTGG ACAGCACCGA GGCGCTGTGC 
CTCGCCATCT CTAGGAACTC CGAGCAGATG GCCAGCCACA GCGCAGTGCT GGAGGCCGGC 
AAAAACCTCT ACACGTTCTG CGTGAGCTAT GTGGATTCCA TCCAGCAAAT GAGGAACAAG 
TTTGCCTTCC GAGAGGCCAT CAACAAACTG GAGAATAATC TCCGGGAGCT TCAGATCTGC 
CCGGCGACAG CAGGCAGTGG TCCAGCGGCC ACTCAGGACT TCAGCAAGCT CCTCAGTTCG 
GTGAAGGAAA TCAGTGACAT AGTGCAGAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypomethylation-mediated H19 overexpression increases the risk of disease evolution through the association with BCR-ABL transcript in chronic myeloid leukemia.
Journal of cellular physiology233(3)2444-2450(2018 Mar)
Zhou JD,Lin J,Zhang TJ,Ma JC,Li XX,Wen XM,Guo H,Xu ZJ,Deng ZQ,Zhang W,Qian J

Atomic view of the energy landscape in the allosteric regulation of Abl kinase.
Nature structural & molecular biology24(11)893-901(2017 Nov)
Saleh T,Rossi P,Kalodimos CG

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ABL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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