POLR2J2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2J2
Entrez Gene ID	246721
Full Name	RNA polymerase II subunit J2
Synonyms	HRPB11B,RPB11b1
General protein information	Preferred Names RNA polymerase II subunit J2 Names DNA-directed RNA polymerase II subunit RPB11-b1 DNA directed RNA polymerase II polypeptide J-related DNA-directed RNA polymerase II subunit J2 RNA polymerase II subunit B11-b1 polymerase (RNA) II (DNA directed) polypeptide J2 polymerase (RNA) II subunit J2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008].
Disorder MIM:	609881

mRNA and Protein(s)

mRNA	Protein	Name
NM_032959.5	NP_116581.3	DNA-directed RNA polymerase II subunit RPB11-b1
NM_032959.6	NP_116581.3	DNA-directed RNA polymerase II subunit RPB11-b1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR2J3	NP_001091084.2
Saccharomyces cerevisiae (baker's yeast)	RPB11	NP_014638.1
Eremothecium gossypii	AGOS_ACL005C	NP_983399.1
Kluyveromyces lactis	KLLA0C06160g	XP_452472.1
Homo sapiens (human)	POLR2J2	NP_116581.3
Schizosaccharomyces pombe (fission yeast)	rpb11	NP_593333.1
Pan troglodytes (chimpanzee)	POLR2J2	XP_003339460.1

Gene Ontology
Bibliography

Related articles in PubMed

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

Human PMS2 gene family: origin, molecular evolution, and biological implications.
Shpakovskii DG, Shematorova EK, Shpakovskii GV
Doklady. Biochemistry and biophysics408175-9(2006 May-Jun)

Distinct regions of RPB11 are required for heterodimerization with RPB3 in human and yeast RNA polymerase II.
Benga WJ, Grandemange S, Shpakovski GV, Shematorova EK, Kedinger C, Vigneron M
Nucleic acids research33(11)3582-90(2005)

The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with a novel variant of DNA-directed RNA polymerase II, subunit 11.
Durrin LK, Krontiris TG
Genomics79(6)809-17(2002 Jun)

A human RNA polymerase II subunit is encoded by a recently generated multigene family.
Grandemange S, Schaller S, Yamano S, Du Manoir S, Shpakovski GV, Mattei MG, Kedinger C, Vigneron M
BMC molecular biology214(2001)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLR2J2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2J2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09250	NM_032959.6 Latest version!	Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu09250	NM_032959.5	Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2J2 ( NM_032959.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_116581.3 Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.

Price & Availability↑

CloneID	OHu09250
Clone ID Related Accession (Same CDS sequence)	NM_032959.5 , NM_032959.6
Accession Version	NM_032959.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB11-b1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105052.3. On Jun 13, 2011 this sequence version replaced NM_032959.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.236358.1, SRR1163655.54026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACGCCC CTCCAGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GATCACCATT 
AACAAGGACA CCAAGGTACC CAAGGCCTGC TTATTCACCA TCAACAAAGA AGACCACACA 
CTGGGAAACA TCATTAAATC ACAACTCCTA AAAGACCCGC AAGTGCTATT TGCTGGCTAC 
AAAGTCCCCC ACCCCTTGGA GCACAAGATC ATCATCCGAG TGCAGACCAC GCCGGACTAC 
AGCCCCCAGG AAGCCTTTAC CAACGCCATC ACCGACCTCA TCAGTGAGCT GTCCCTGCTG 
GAGGAGCGCT TCCGGACGTG CCTGCTTCCC CTTCGCCTTC TGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116581.3
CDS	129..476
Translation	MNAPPAFESFLLFEGEKITINKDTKVPKACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLLPLRLLP

Target ORF information:

RefSeq Version	NM_032959.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032959.5

ORF Insert Sequence:

ATGAACGCCC CTCCAGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GATCACCATT 
AACAAGGACA CCAAGGTACC CAAGGCCTGC TTATTCACCA TCAACAAAGA AGACCACACA 
CTGGGAAACA TCATTAAATC ACAACTCCTA AAAGACCCGC AAGTGCTATT TGCTGGCTAC 
AAAGTCCCCC ACCCCTTGGA GCACAAGATC ATCATCCGAG TGCAGACCAC GCCGGACTAC 
AGCCCCCAGG AAGCCTTTAC CAACGCCATC ACCGACCTCA TCAGTGAGCT GTCCCTGCTG 
GAGGAGCGCT TCCGGACGTG CCTGCTTCCC CTTCGCCTTC TGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2J2 ( NM_032959.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_116581.3 Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.

Price & Availability↑

CloneID	OHu09250
Clone ID Related Accession (Same CDS sequence)	NM_032959.5 , NM_032959.6
Accession Version	NM_032959.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB11-b1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105052.3. On Jun 2, 2019 this sequence version replaced NM_032959.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.326503.1, SRR3476690.899326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACGCCC CTCCAGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GATCACCATT 
AACAAGGACA CCAAGGTACC CAAGGCCTGC TTATTCACCA TCAACAAAGA AGACCACACA 
CTGGGAAACA TCATTAAATC ACAACTCCTA AAAGACCCGC AAGTGCTATT TGCTGGCTAC 
AAAGTCCCCC ACCCCTTGGA GCACAAGATC ATCATCCGAG TGCAGACCAC GCCGGACTAC 
AGCCCCCAGG AAGCCTTTAC CAACGCCATC ACCGACCTCA TCAGTGAGCT GTCCCTGCTG 
GAGGAGCGCT TCCGGACGTG CCTGCTTCCC CTTCGCCTTC TGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116581.3
CDS	135..482
Translation	MNAPPAFESFLLFEGEKITINKDTKVPKACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLLPLRLLP

Target ORF information:

RefSeq Version	NM_032959.6
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit J2 (POLR2J2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032959.6

ORF Insert Sequence:

ATGAACGCCC CTCCAGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GATCACCATT 
AACAAGGACA CCAAGGTACC CAAGGCCTGC TTATTCACCA TCAACAAAGA AGACCACACA 
CTGGGAAACA TCATTAAATC ACAACTCCTA AAAGACCCGC AAGTGCTATT TGCTGGCTAC 
AAAGTCCCCC ACCCCTTGGA GCACAAGATC ATCATCCGAG TGCAGACCAC GCCGGACTAC 
AGCCCCCAGG AAGCCTTTAC CAACGCCATC ACCGACCTCA TCAGTGAGCT GTCCCTGCTG 
GAGGAGCGCT TCCGGACGTG CCTGCTTCCC CTTCGCCTTC TGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

Human PMS2 gene family: origin, molecular evolution, and biological implications.
Doklady. Biochemistry and biophysics408175-9(2006 May-Jun)
Shpakovskii DG,Shematorova EK,Shpakovskii GV

Distinct regions of RPB11 are required for heterodimerization with RPB3 in human and yeast RNA polymerase II.
Nucleic acids research33(11)3582-90(2005)
Benga WJ,Grandemange S,Shpakovski GV,Shematorova EK,Kedinger C,Vigneron M

The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with a novel variant of DNA-directed RNA polymerase II, subunit 11.
Genomics79(6)809-17(2002 Jun)
Durrin LK,Krontiris TG

A human RNA polymerase II subunit is encoded by a recently generated multigene family.
BMC molecular biology214(2001)
Grandemange S,Schaller S,Yamano S,Du Manoir S,Shpakovski GV,Mattei MG,Kedinger C,Vigneron M

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POLR2J2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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