SPDYA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPDYA
Entrez Gene ID	245711
Full Name	speedy/RINGO cell cycle regulator family member A
Synonyms	RINGO3,RINGOA,SPDY1,SPY1
General protein information	Preferred Names speedy/RINGO cell cycle regulator family member A Names speedy protein A RINGO A hSpy/Ringo A rapid inducer of G2/M progression in oocytes A speedy homolog A speedy-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.2
Disorder MIM:	614029

mRNA and Protein(s)

mRNA	Protein	Name
NM_182756.4	NP_877433.2	speedy protein A isoform 1
NM_001142634.1	NP_001136106.1	speedy protein A isoform 1
NM_001142634.2	NP_001136106.1	speedy protein A isoform 1
NM_182756.3	NP_877433.2	speedy protein A isoform 1
NM_001008779.1	NP_001008779.1	speedy protein A isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPDYA	NP_001178076.2
Mus musculus (house mouse)	Spdya	NP_083530.1
	SPDYA	XP_001102674.1
Danio rerio (zebrafish)	spdya	NP_001006091.1
Xenopus tropicalis (tropical clawed frog)	spdya	NP_001106435.1
Rattus norvegicus (Norway rat)	Spdya	NP_620210.1
Homo sapiens (human)	SPDYA	NP_001136106.1
Pan troglodytes (chimpanzee)	SPDYA	XP_003308993.1
Canis lupus familiaris (dog)	SPDYA	XP_003432227.2
Gallus gallus (chicken)	SPDYA	XP_004940252.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins.
McGrath DA, Fifield BA, Marceau AH, Tripathi S, Porter LA, Rubin SM
The EMBO journal36(15)2251-2262(2017 08)

The cyclin-like protein, SPY1, regulates the ER? and ERK1/2 pathways promoting tamoxifen resistance.
Ferraiuolo RM, Tubman J, Sinha I, Hamm C, Porter LA
Oncotarget8(14)23337-23352(2017 Apr)

CRMP1 Interacted with Spy1 During the Collapse of Growth Cones Induced by Sema3A and Acted on Regeneration After Sciatic Nerve Crush.
Yao L, Liu YH, Li X, Ji YH, Yang XJ, Hang XT, Ding ZM, Liu F, Wang YH, Shen AG
Molecular neurobiology53(2)879-93(2016 Mar)

Spy1 participates in the proliferation and apoptosis of epithelial ovarian cancer.
Lu S, Liu R, Su M, Wei Y, Yang S, He S, Wang X, Qiang F, Chen C, Zhao S, Zhang W, Xu P, Mao G
Journal of molecular histology47(1)47-57(2016 Feb)

Spy1 induces de-ubiquitinating of RIP1 arrest and confers glioblastoma's resistance to tumor necrosis factor (TNF-?)-induced apoptosis through suppressing the association of CLIPR-59 and CYLD.
Ding Z, Liu Y, Yao L, Wang D, Zhang J, Cui G, Yang X, Huang X, Liu F, Shen A
Cell cycle (Georgetown, Tex.)14(13)2149-59(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors find that Spy1 confers structural changes to Cdk2 that obviate the requirement of Cdk activation loop phosphorylation.
Title: Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins.

Authors demonstrate that mammalian Spy1-mediated ERK activation increases ligand-independent phosphorylation and activation of estrogen receptor alpha, correlating with a decrease in tamoxifen sensitivity.
Title: The cyclin-like protein, SPY1, regulates the ER? and ERK1/2 pathways promoting tamoxifen resistance.

CRMP1 interacted with Spy1 which would disturb the association of CRMP1 with actin and was involved in the collapse of growth cones induced by Sema3A and regeneration after sciatic nerve crush.
Title: CRMP1 Interacted with Spy1 During the Collapse of Growth Cones Induced by Sema3A and Acted on Regeneration After Sciatic Nerve Crush.

Spy1 participates in the proliferation and apoptosis of epithelial ovarian cancer.
Title: Spy1 participates in the proliferation and apoptosis of epithelial ovarian cancer.

A novel Spy1-CLIPR-59 interplay was identified in glioblastoma resistance to TNF-alpha.
Title: Spy1 induces de-ubiquitinating of RIP1 arrest and confers glioblastoma's resistance to tumor necrosis factor (TNF-?)-induced apoptosis through suppressing the association of CLIPR-59 and CYLD.

The following SPDYA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPDYA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14043	NM_001142634.2 Latest version!	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu14043	NM_182756.4 Latest version!	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu14157	NM_001008779.1 Latest version!	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu14043	NM_001142634.1	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu14043	NM_182756.3	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPDYA ( NM_182756.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_877433.2 Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14043
Clone ID Related Accession (Same CDS sequence)	NM_182756.4 , NM_001142634.1 , NM_001142634.2 , NM_182756.3
Accession Version	NM_182756.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	speedy protein A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC093005.1, BC047224.1 and AY820304.1. On Nov 22, 2018 this sequence version replaced NM_182756.3. Transcript Variant: This variant (1) lacks a segment in the 5' UTR, compared to variant 3. Variants 1 and 3 both encode the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.122914.1, BC047224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334056.10/ ENSP00000335628.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877433.2
CDS	207..1148
Translation	MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

Target ORF information:

RefSeq Version	NM_182756.4
Organism	Homo sapiens(human)
Definition	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182756.4

ORF Insert Sequence:

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPDYA ( NM_001142634.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136106.1 Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14043
Clone ID Related Accession (Same CDS sequence)	NM_182756.4 , NM_001142634.1 , NM_001142634.2 , NM_182756.3
Accession Version	NM_001142634.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	speedy protein A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC093005.1, AY820304.1 and BC047224.1. Transcript Variant: This variant (3) represents the longest transcript. Variants 1 and 3 both encode the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136106.1
CDS	133..1074
Translation	MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

Target ORF information:

RefSeq Version	NM_001142634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142634.1

ORF Insert Sequence:

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPDYA ( NM_001142634.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136106.1 Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14043
Clone ID Related Accession (Same CDS sequence)	NM_182756.4 , NM_001142634.1 , NM_001142634.2 , NM_182756.3
Accession Version	NM_001142634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	speedy protein A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC093005.1, AY820304.1 and BC047224.1. On May 31, 2019 this sequence version replaced NM_001142634.1. Transcript Variant: This variant (3) represents the longest transcript. Variants 1 and 3 both encode the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ891528.1, BC093005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136106.1
CDS	133..1074
Translation	MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

Target ORF information:

RefSeq Version	NM_001142634.2
Organism	Homo sapiens(human)
Definition	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142634.2

ORF Insert Sequence:

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPDYA ( NM_182756.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_877433.2 Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14043
Clone ID Related Accession (Same CDS sequence)	NM_182756.4 , NM_001142634.1 , NM_001142634.2 , NM_182756.3
Accession Version	NM_182756.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	speedy protein A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC093005.1, BC047224.1 and AY820304.1. On Dec 13, 2008 this sequence version replaced NM_182756.2. Transcript Variant: This variant (1) lacks a segment in the 5' UTR, compared to variant 3. Variants 1 and 3 both encode the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877433.2
CDS	207..1148
Translation	MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

Target ORF information:

RefSeq Version	NM_182756.3
Organism	Homo sapiens(human)
Definition	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182756.3

ORF Insert Sequence:

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG TAGTCAATGA CCATCAATCA AATAAAGGAA AGAAAACTAA TTTCTTGAAG 
AAAGACAAAT CTATGGAGTG GTTTACAGGA AGTGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPDYA ( NM_001008779.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008779.1 Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 2, mRNA.

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CloneID	OHu14157
Clone ID Related Accession (Same CDS sequence)	NM_001008779.1
Accession Version	NM_001008779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	speedy protein A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC047224.1, AY820303.1 and AA625975.1. Transcript Variant: This variant (2) lacks some 5' UTR sequence and uses a different splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY820303.1, BI831897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG GTATGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008779.1
CDS	19..879
Translation	MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEGMI

Target ORF information:

RefSeq Version	NM_001008779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens speedy/RINGO cell cycle regulator family member A (SPDYA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008779.1

ORF Insert Sequence:

ATGAGGCACA ATCAGATGTG TTGTGAGACA CCACCTACTG TCACTGTTTA TGTAAAATCA 
GGGTCAAATA GATCACATCA GCCTAAAAAG CCCATTACTC TGAAGCGTCC TATTTGTAAA 
GATAATTGGC AAGCATTTGA AAAAAATACA CATAATAACA ACAAATCTAA ACGCCCCAAA 
GGACCTTGTC TGGTTATACA GCGTCAGGAT ATGACTGCTT TCTTTAAATT ATTTGATGAC 
GATTTAATTC AAGATTTCTT GTGGATGGAC TGCTGCTGTA AAATTGCAGA CAAGTATCTT 
TTGGCTATGA CCTTTGTTTA TTTCAAGAGG GCTAAATTTA CTATAAGTGA GCATACCAGG 
ATAAATTTCT TTATTGCTCT GTATCTGGCT AATACAGTTG AAGAAGATGA AGAAGAAACC 
AAGTACGAAA TTTTTCCATG GGCTTTAGGG AAAAACTGGA GAAAATTGTT CCCTAATTTC 
TTAAAGTTAA GGGACCAGCT CTGGGATAGA ATTGACTATA GGGCTATTGT AAGCAGGCGA 
TGTTGTGAGG AGGTTATGGC CATTGCACCA ACCCATTATA TCTGGCAAAG AGAACGTTCT 
GTTCATCACA GTGGAGCTGT CAGAAACTAC AACAGAGATG AAGTTCAGCT GCCCCGGGGA 
CCTAGTGCCA CACCAGTAGA TTGTTCACTC TGTGGTAAAA AAAGAAGATA TGTTAGACTG 
GGATTGTCTT CATCATCATC TTTATCCAGT CATACAGCAG GGGTGACAGA AAAACATTCT 
CAGGACTCAT ACAACTCACT GTCAATGGAC ATAATAGGTG ATCCTTCTCA AGCTTATACT 
GGTTCTGAAG GTATGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins.
The EMBO journal36(15)2251-2262(2017 08)
McGrath DA,Fifield BA,Marceau AH,Tripathi S,Porter LA,Rubin SM

The cyclin-like protein, SPY1, regulates the ER? and ERK1/2 pathways promoting tamoxifen resistance.
Oncotarget8(14)23337-23352(2017 Apr)
Ferraiuolo RM,Tubman J,Sinha I,Hamm C,Porter LA

CRMP1 Interacted with Spy1 During the Collapse of Growth Cones Induced by Sema3A and Acted on Regeneration After Sciatic Nerve Crush.
Molecular neurobiology53(2)879-93(2016 Mar)
Yao L,Liu YH,Li X,Ji YH,Yang XJ,Hang XT,Ding ZM,Liu F,Wang YH,Shen AG

Spy1 participates in the proliferation and apoptosis of epithelial ovarian cancer.
Journal of molecular histology47(1)47-57(2016 Feb)
Lu S,Liu R,Su M,Wei Y,Yang S,He S,Wang X,Qiang F,Chen C,Zhao S,Zhang W,Xu P,Mao G

Spy1 induces de-ubiquitinating of RIP1 arrest and confers glioblastoma's resistance to tumor necrosis factor (TNF-?)-induced apoptosis through suppressing the association of CLIPR-59 and CYLD.
Cell cycle (Georgetown, Tex.)14(13)2149-59(2015)
Ding Z,Liu Y,Yao L,Wang D,Zhang J,Cui G,Yang X,Huang X,Liu F,Shen A

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SPDYA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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