PPP1R15A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R15A
Entrez Gene ID	23645
Full Name	protein phosphatase 1 regulatory subunit 15A
Synonyms	GADD34
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 15A Names protein phosphatase 1 regulatory subunit 15A growth arrest and DNA damage-inducible protein GADD34 growth arrest and DNA-damage-inducible 34 myeloid differentiation primary response protein MyD116 homolog protein phosphatase 1, regulatory (inhibitor) subunit 15A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Disorder MIM:	611048

mRNA and Protein(s)

mRNA	Protein	Name
NM_014330.3	NP_055145.3	protein phosphatase 1 regulatory subunit 15A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPP1R15A	XP_512805.2
	PPP1R15A	XP_001114376.1
Bos taurus (cattle)	PPP1R15A	NP_001039643.1
Homo sapiens (human)	PPP1R15A	NP_055145.3
Mus musculus (house mouse)	Ppp1r15a	NP_032680.1

Gene Ontology
Bibliography

Related articles in PubMed

Relationship between PPP1R15A gene polymorphism (rs611251) and Epstein-Barr virus-associated tumors.
Song Y, Liu S, Zhao Z, Zhang Y, Yang Y, Luo B
Acta virologica61(4)445-452(2017)

Inhibition of IRE1 modifies the hypoxic regulation of GADD family gene expressions in U87 glioma cells.
Minchenko OH, Kryvdiuk IV, Riabovol OO, Minchenko DO, Danilovskyi SV, Ratushna OO
Ukrainian biochemical journal88(2)25-34(2016 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reduction of GADD34 expression significantly suppressed tumor, and resulted in decreased accumulation of MDSCs and T-cells, and inhibition of GADD34 reduced secretion of vascular epithelial growth factor alpha and transforming growth factor beta by MDSCs
Title: GADD34 Promotes Tumor Growth by Inducing Myeloid-derived Suppressor Cells.

ANXA11 rs1049550 and PPP1R15A rs557806 may improve the identification of mCRC patients sensitive to bevacizumab regimens, and further validation is required in large cohorts
Title: Feasibility of novel PPP1R15A and proposed ANXA11 single nucleotide polymorphisms as predictive markers for bevacizumab regimen in metastatic colorectal cancer.

Inhibition of IRE1 modifies the hypoxic regulation of GADD34 family gene expression in cultured glioma cells.
Title: Inhibition of IRE1 modifies the hypoxic regulation of GADD family gene expressions in U87 glioma cells.

GADD34 constitutes a mechanistic link between endoplasmic reticulum stress and mTOR inactivation, therefore promotes cell survival during endoplasmic reticulum stress.
Title: GADD34 Keeps the mTOR Pathway Inactivated in Endoplasmic Reticulum Stress Related Autophagy.

Data of this study strengthen the evidence of an unfolded protein response during the course of RA and provide an insight of the potential interest in GADD34 as a relevant marker for RA.
Title: Unfolded protein response gene GADD34 is overexpressed in rheumatoid arthritis and related to the presence of circulating anti-citrullinated protein antibodies.

The following PPP1R15A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R15A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu17374	NM_014330.3 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 15A (PPP1R15A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R15A ( NM_014330.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055145.3 Homo sapiens protein phosphatase 1 regulatory subunit 15A (PPP1R15A), mRNA.

Price & Availability↑

CloneID	OHu17374
Clone ID Related Accession (Same CDS sequence)	NM_014330.3
Accession Version	NM_014330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 15A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA484808.1 and AK225632.1. On Sep 26, 2007 this sequence version replaced NM_014330.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225632.1, SRR1803612.227044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript regulatory uORF :: PMID: 19131336 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCAG GCCAAGCACC CCATCAGGCT ACCCCGTGGA GGGATGCCCA CCCTTTCTTC 
CTCCTGTCCC CAGTGATGGG CCTCCTCAGC CGCGCCTGGA GCCGCCTGAG GGGCCTGGGA 
CCTCTAGAGC CCTGGCTGGT GGAAGCAGTA AAAGGAGCAG CTCTGGTAGA AGCTGGCCTG 
GAGGGAGAAG CTAGGACTCC TCTGGCAATC CCCCATACCC CTTGGGGCAG ACGCCCTGAA 
GAGGAGGCTG AAGACAGTGG AGGCCCTGGA GAGGACAGAG AAACACTGGG GCTGAAAACC 
AGCAGTTCCC TTCCTGAAGC CTGGGGACTT TTGGATGATG ATGATGGCAT GTATGGTGAG 
CGAGAGGCAA CCAGTGTCCC TAGAGGGCAG GGAAGTCAAT TTGCAGATGG CCAGCGTGCT 
CCCCTGTCTC CCAGCCTTCT GATAAGGACA CTGCAAGGTT CTGATAAGAA CCCAGGGGAG 
GAGAAAGCCG AGGAAGAGGG AGTTGCTGAA GAGGAGGGAG TTAACAAGTT CTCTTATCCA 
CCATCACACC GGGAGTGTTG TCCAGCCGTG GAGGAGGAGG ACGATGAAGA AGCTGTAAAG 
AAAGAAGCTC ACAGAACCTC TACTTCTGCC TTGTCTCCAG GATCCAAGCC CAGCACTTGG 
GTGTCTTGCC CAGGGGAGGA AGAGAATCAA GCCACGGAGG ATAAAAGAAC AGAAAGAAGT 
AAAGGAGCCA GGAAGACCTC CGTGTCCCCC CGATCTTCAG GCTCCGACCC CAGGTCCTGG 
GAGTATCGTT CAGGAGAGGC GTCCGAGGAG AAGGAGGAAA AGGCACACAA AGAAACTGGG 
AAAGGAGAAG CTGCCCCAGG GCCGCAATCC TCAGCCCCAG CCCAGAGGCC CCAGCTCAAG 
TCCTGGTGGT GCCAACCCAG TGATGAAGAG GAGGGTGAGG TCAAGGCTTT GGGGGCAGCT 
GAGAAGGATG GAGAAGCTGA GTGTCCTCCC TGCATCCCCC CACCAAGTGC CTTCCTGAAG 
GCCTGGGTGT ATTGGCCAGG AGAGGACACA GAGGAAGAGG AAGATGAGGA AGAAGATGAG 
GACAGTGACT CTGGATCAGA TGAGGAAGAG GGAGAAGCTG AGGCTTCCTC TTCCACTCCT 
GCTACAGGTG TCTTCTTGAA GTCCTGGGTC TATCAGCCAG GAGAGGACAC AGAGGAGGAG 
GAAGATGAGG ACAGTGATAC AGGATCAGCC GAGGATGAAA GAGAAGCTGA GACTTCTGCT 
TCCACACCCC CTGCAAGTGC TTTCTTGAAG GCCTGGGTGT ATCGGCCAGG AGAGGACACG 
GAGGAGGAGG AAGATGAGGA TGTGGATAGT GAGGATAAGG AAGATGATTC AGAAGCAGCC 
TTGGGAGAAG CTGAGTCAGA CCCACATCCC TCCCACCCGG ACCAGAGGGC CCACTTCAGG 
GGCTGGGGAT ATCGACCTGG AAAAGAGACA GAGGAAGAGG AAGCTGCTGA GGACTGGGGA 
GAAGCTGAGC CCTGCCCCTT CCGAGTGGCC ATCTATGTAC CTGGAGAGAA GCCACCGCCT 
CCCTGGGCTC CTCCTAGGCT GCCCCTCCGA CTGCAAAGGC GGCTCAAGCG CCCAGAAACC 
CCTACTCATG ATCCGGACCC TGAGACTCCC CTAAAGGCCA GAAAGGTGCG CTTCTCCGAG 
AAGGTCACTG TCCATTTCCT GGCTGTCTGG GCAGGGCCGG CCCAGGCCGC CCGCCAGGGC 
CCCTGGGAGC AGCTTGCTCG GGATCGCAGC CGCTTCGCAC GCCGCATCAC CCAGGCCCAG 
GAGGAGCTGA GCCCCTGCCT CACCCCTGCT GCCCGGGCCA GAGCCTGGGC ACGCCTCAGG 
AACCCACCTT TAGCCCCCAT CCCTGCCCTC ACCCAGACCT TGCCTTCCTC CTCTGTCCCT 
TCGTCCCCAG TCCAGACCAC GCCCTTGAGC CAAGCTGTGG CCACACCTTC CCGCTCGTCT 
GCTGCTGCAG CGGCTGCCCT GGACCTCAGT GGGAGGCGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055145.3
CDS	270..2294
Translation	MAPGQAPHQATPWRDAHPFFLLSPVMGLLSRAWSRLRGLGPLEPWLVEAVKGAALVEAGLEGEARTPLAIPHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRAPLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSALSPGSKPSTWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETGKGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDTEEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG

Target ORF information:

RefSeq Version	NM_014330.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 15A (PPP1R15A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014330.3

ORF Insert Sequence:

ATGGCCCCAG GCCAAGCACC CCATCAGGCT ACCCCGTGGA GGGATGCCCA CCCTTTCTTC 
CTCCTGTCCC CAGTGATGGG CCTCCTCAGC CGCGCCTGGA GCCGCCTGAG GGGCCTGGGA 
CCTCTAGAGC CCTGGCTGGT GGAAGCAGTA AAAGGAGCAG CTCTGGTAGA AGCTGGCCTG 
GAGGGAGAAG CTAGGACTCC TCTGGCAATC CCCCATACCC CTTGGGGCAG ACGCCCTGAA 
GAGGAGGCTG AAGACAGTGG AGGCCCTGGA GAGGACAGAG AAACACTGGG GCTGAAAACC 
AGCAGTTCCC TTCCTGAAGC CTGGGGACTT TTGGATGATG ATGATGGCAT GTATGGTGAG 
CGAGAGGCAA CCAGTGTCCC TAGAGGGCAG GGAAGTCAAT TTGCAGATGG CCAGCGTGCT 
CCCCTGTCTC CCAGCCTTCT GATAAGGACA CTGCAAGGTT CTGATAAGAA CCCAGGGGAG 
GAGAAAGCCG AGGAAGAGGG AGTTGCTGAA GAGGAGGGAG TTAACAAGTT CTCTTATCCA 
CCATCACACC GGGAGTGTTG TCCAGCCGTG GAGGAGGAGG ACGATGAAGA AGCTGTAAAG 
AAAGAAGCTC ACAGAACCTC TACTTCTGCC TTGTCTCCAG GATCCAAGCC CAGCACTTGG 
GTGTCTTGCC CAGGGGAGGA AGAGAATCAA GCCACGGAGG ATAAAAGAAC AGAAAGAAGT 
AAAGGAGCCA GGAAGACCTC CGTGTCCCCC CGATCTTCAG GCTCCGACCC CAGGTCCTGG 
GAGTATCGTT CAGGAGAGGC GTCCGAGGAG AAGGAGGAAA AGGCACACAA AGAAACTGGG 
AAAGGAGAAG CTGCCCCAGG GCCGCAATCC TCAGCCCCAG CCCAGAGGCC CCAGCTCAAG 
TCCTGGTGGT GCCAACCCAG TGATGAAGAG GAGGGTGAGG TCAAGGCTTT GGGGGCAGCT 
GAGAAGGATG GAGAAGCTGA GTGTCCTCCC TGCATCCCCC CACCAAGTGC CTTCCTGAAG 
GCCTGGGTGT ATTGGCCAGG AGAGGACACA GAGGAAGAGG AAGATGAGGA AGAAGATGAG 
GACAGTGACT CTGGATCAGA TGAGGAAGAG GGAGAAGCTG AGGCTTCCTC TTCCACTCCT 
GCTACAGGTG TCTTCTTGAA GTCCTGGGTC TATCAGCCAG GAGAGGACAC AGAGGAGGAG 
GAAGATGAGG ACAGTGATAC AGGATCAGCC GAGGATGAAA GAGAAGCTGA GACTTCTGCT 
TCCACACCCC CTGCAAGTGC TTTCTTGAAG GCCTGGGTGT ATCGGCCAGG AGAGGACACG 
GAGGAGGAGG AAGATGAGGA TGTGGATAGT GAGGATAAGG AAGATGATTC AGAAGCAGCC 
TTGGGAGAAG CTGAGTCAGA CCCACATCCC TCCCACCCGG ACCAGAGGGC CCACTTCAGG 
GGCTGGGGAT ATCGACCTGG AAAAGAGACA GAGGAAGAGG AAGCTGCTGA GGACTGGGGA 
GAAGCTGAGC CCTGCCCCTT CCGAGTGGCC ATCTATGTAC CTGGAGAGAA GCCACCGCCT 
CCCTGGGCTC CTCCTAGGCT GCCCCTCCGA CTGCAAAGGC GGCTCAAGCG CCCAGAAACC 
CCTACTCATG ATCCGGACCC TGAGACTCCC CTAAAGGCCA GAAAGGTGCG CTTCTCCGAG 
AAGGTCACTG TCCATTTCCT GGCTGTCTGG GCAGGGCCGG CCCAGGCCGC CCGCCAGGGC 
CCCTGGGAGC AGCTTGCTCG GGATCGCAGC CGCTTCGCAC GCCGCATCAC CCAGGCCCAG 
GAGGAGCTGA GCCCCTGCCT CACCCCTGCT GCCCGGGCCA GAGCCTGGGC ACGCCTCAGG 
AACCCACCTT TAGCCCCCAT CCCTGCCCTC ACCCAGACCT TGCCTTCCTC CTCTGTCCCT 
TCGTCCCCAG TCCAGACCAC GCCCTTGAGC CAAGCTGTGG CCACACCTTC CCGCTCGTCT 
GCTGCTGCAG CGGCTGCCCT GGACCTCAGT GGGAGGCGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Relationship between PPP1R15A gene polymorphism (rs611251) and Epstein-Barr virus-associated tumors.
Acta virologica61(4)445-452(2017)
Song Y,Liu S,Zhao Z,Zhang Y,Yang Y,Luo B

Inhibition of IRE1 modifies the hypoxic regulation of GADD family gene expressions in U87 glioma cells.
Ukrainian biochemical journal88(2)25-34(2016 Mar-Apr)
Minchenko OH,Kryvdiuk IV,Riabovol OO,Minchenko DO,Danilovskyi SV,Ratushna OO

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PPP1R15A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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