PHLDA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHLDA3
Entrez Gene ID	23612
Full Name	pleckstrin homology like domain family A member 3
Synonyms	TIH1
General protein information	Preferred Names pleckstrin homology like domain family A member 3 Names pleckstrin homology-like domain family A member 3 TDAG51/Ipl homolog 1 pleckstrin homology-like domain, family A, member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Disorder MIM:	607054

mRNA and Protein(s)

mRNA	Protein	Name
NM_012396.4	NP_036528.1	pleckstrin homology-like domain family A member 3
NM_012396.5	NP_036528.1	pleckstrin homology-like domain family A member 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHLDA3	XP_003308734.1
	LOC709782	XP_001098353.1
Rattus norvegicus (Norway rat)	Phlda3	NP_001012206.1
Homo sapiens (human)	PHLDA3	NP_036528.1
Mus musculus (house mouse)	Phlda3	NP_038778.1
Gallus gallus (chicken)	LOC100859283	XP_003642782.1
Danio rerio (zebrafish)	phlda3	NP_001002455.1

Gene Ontology
Bibliography

Related articles in PubMed

PHLDA3 impedes somatic cell reprogramming by activating Akt-GSK3? pathway.
Qiao M, Wu M, Shi R, Hu W
Scientific reports7(1)2832(2017 Jun)

Low PHLDA3 expression in oesophageal squamous cell carcinomas is associated with poor prognosis.
Muroi H, Nakajima M, Satomura H, Takahashi M, Yamaguchi S, Sasaki K, Yokobori T, Miyazaki T, Kuwano H, Kato H
Anticancer research35(2)949-54(2015 Feb)

PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors.
Ohki R, Saito K, Chen Y, Kawase T, Hiraoka N, Saigawa R, Minegishi M, Aita Y, Yanai G, Shimizu H, Yachida S, Sakata N, Doi R, Kosuge T, Shimada K, Tycko B, Tsukada T, Kanai Y, Sumi S, Namiki H, Taya Y, Shibata T, Nakagama H
Proceedings of the National Academy of Sciences of the United States of America111(23)E2404-13(2014 Jun)

Expressional and mutational analysis of PHLDA3 gene in common human cancers.
Yoo NJ, Kim YR, Lee SH
Pathology43(5)510-1(2011 Aug)

PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.
Kawase T, Ohki R, Shibata T, Tsutsumi S, Kamimura N, Inazawa J, Ohta T, Ichikawa H, Aburatani H, Tashiro F, Taya Y
Cell136(3)535-50(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low PHLDA3 expression in ESCC may be predictive of tumor recurrence suggesting that Akt activation may be a therapeutic target in ESCCs
Title: Low PHLDA3 expression in oesophageal squamous cell carcinomas is associated with poor prognosis.

Results indicate that tumor suppressor PHLDA3-mediated pathway is important in the development of pancreatic neuroendocrine tumors (PanNETs).
Title: PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors.

findings indicate somatic mutation of PHLDA3 is rare in common cancer types; PHLDA3 expression was lost in 22% of prostate cancers; these results indicate that PHLDA3 are altered in prostate cancers by loss of expression
Title: Expressional and mutational analysis of PHLDA3 gene in common human cancers.

Study identifies PHLDA3 as a p53 target gene that encodes a PH domain-only protein and finds that PHLDA3 competes with the PH domain of Akt for binding of membrane lipids, inhibiting Akt translocation to the cellular membrane and activation.
Title: PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.

The following PHLDA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13273	NM_012396.5 Latest version!	Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13273	NM_012396.4	Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLDA3 ( NM_012396.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036528.1 Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13273
Clone ID Related Accession (Same CDS sequence)	NM_012396.4 , NM_012396.5
Accession Version	NM_012396.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family A member 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC068273.1, BC014390.1, AF151100.1, AC096677.2, BE778799.1, BX090314.1, BE892338.1, BQ000725.1, AI624156.1 and BC033874.1. On May 28, 2015 this sequence version replaced NM_012396.3. Transcript Variant: This variant (1) encodes the functional protein. CCDS Note: This CCDS ID represents the protein described in PMID: 19203586 and is supported by BC014390.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 19203586. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. ##Evidence-Data-START## Transcript exon combination :: BC068273.1, SRR1803611.126849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 19203586 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGGCGG CGGCGACGGC TACCGTGCTC AAGGAGGGCG TGCTGGAGAA GCGCAGCGGC 
GGGCTGCTGC AGCTGTGGAA GCGGAAGCGC TGCGTCCTCA CCGAACGCGG GCTGCAGCTC 
TTCGAGGCCA AGGGCACGGG CGGCCGGCCC AAGGAGCTCA GCTTCGCCCG CATCAAGGCC 
GTGGAGTGCG TGGAGAGCAC CGGGCGCCAC ATCTACTTCA CGCTGGTGAC CGAAGGGGGC 
GGCGAGATCG ACTTCCGCTG CCCCCTGGAA GATCCCGGCT GGAACGCCCA GATCACCCTA 
GGCCTGGTCA AGTTCAAGAA CCAGCAGGCC ATCCAGACAG TGCGGGCCCG GCAGAGCCTC 
GGGACCGGGA CCCTCGTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036528.1
CDS	386..769
Translation	MTAAATATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGTGGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGLVKFKNQQAIQTVRARQSLGTGTLVS

Target ORF information:

RefSeq Version	NM_012396.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012396.4

ORF Insert Sequence:

ATGACGGCGG CGGCGACGGC TACCGTGCTC AAGGAGGGCG TGCTGGAGAA GCGCAGCGGC 
GGGCTGCTGC AGCTGTGGAA GCGGAAGCGC TGCGTCCTCA CCGAACGCGG GCTGCAGCTC 
TTCGAGGCCA AGGGCACGGG CGGCCGGCCC AAGGAGCTCA GCTTCGCCCG CATCAAGGCC 
GTGGAGTGCG TGGAGAGCAC CGGGCGCCAC ATCTACTTCA CGCTGGTGAC CGAAGGGGGC 
GGCGAGATCG ACTTCCGCTG CCCCCTGGAA GATCCCGGCT GGAACGCCCA GATCACCCTA 
GGCCTGGTCA AGTTCAAGAA CCAGCAGGCC ATCCAGACAG TGCGGGCCCG GCAGAGCCTC 
GGGACCGGGA CCCTCGTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDA3 ( NM_012396.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036528.1 Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13273
Clone ID Related Accession (Same CDS sequence)	NM_012396.4 , NM_012396.5
Accession Version	NM_012396.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	pleckstrin homology-like domain family A member 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC096677.2, BC068273.1, BC014390.1, AF151100.1, BE778799.1, BX090314.1, BE892338.1, BQ000725.1, AI624156.1 and BC033874.1. On May 17, 2019 this sequence version replaced NM_012396.4. Transcript Variant: This variant (1) encodes the functional protein. CCDS Note: This CCDS ID represents the protein described in PMID: 19203586 and is supported by BC014390.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 19203586. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.126849.1, SRR1803616.230560.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367311.5/ ENSP00000356280.3 NMD candidate :: PMID: 19203586 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGGCGG CGGCGACGGC TACCGTGCTC AAGGAGGGCG TGCTGGAGAA GCGCAGCGGC 
GGGCTGCTGC AGCTGTGGAA GCGGAAGCGC TGCGTCCTCA CCGAACGCGG GCTGCAGCTC 
TTCGAGGCCA AGGGCACGGG CGGCCGGCCC AAGGAGCTCA GCTTCGCCCG CATCAAGGCC 
GTGGAGTGCG TGGAGAGCAC CGGGCGCCAC ATCTACTTCA CGCTGGTGAC CGAAGGGGGC 
GGCGAGATCG ACTTCCGCTG CCCCCTGGAA GATCCCGGCT GGAACGCCCA GATCACCCTA 
GGCCTGGTCA AGTTCAAGAA CCAGCAGGCC ATCCAGACAG TGCGGGCCCG GCAGAGCCTC 
GGGACCGGGA CCCTCGTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036528.1
CDS	403..786
Translation	MTAAATATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGTGGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGLVKFKNQQAIQTVRARQSLGTGTLVS

Target ORF information:

RefSeq Version	NM_012396.5
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology like domain family A member 3 (PHLDA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012396.5

ORF Insert Sequence:

ATGACGGCGG CGGCGACGGC TACCGTGCTC AAGGAGGGCG TGCTGGAGAA GCGCAGCGGC 
GGGCTGCTGC AGCTGTGGAA GCGGAAGCGC TGCGTCCTCA CCGAACGCGG GCTGCAGCTC 
TTCGAGGCCA AGGGCACGGG CGGCCGGCCC AAGGAGCTCA GCTTCGCCCG CATCAAGGCC 
GTGGAGTGCG TGGAGAGCAC CGGGCGCCAC ATCTACTTCA CGCTGGTGAC CGAAGGGGGC 
GGCGAGATCG ACTTCCGCTG CCCCCTGGAA GATCCCGGCT GGAACGCCCA GATCACCCTA 
GGCCTGGTCA AGTTCAAGAA CCAGCAGGCC ATCCAGACAG TGCGGGCCCG GCAGAGCCTC 
GGGACCGGGA CCCTCGTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PHLDA3 impedes somatic cell reprogramming by activating Akt-GSK3? pathway.
Scientific reports7(1)2832(2017 Jun)
Qiao M,Wu M,Shi R,Hu W

Low PHLDA3 expression in oesophageal squamous cell carcinomas is associated with poor prognosis.
Anticancer research35(2)949-54(2015 Feb)
Muroi H,Nakajima M,Satomura H,Takahashi M,Yamaguchi S,Sasaki K,Yokobori T,Miyazaki T,Kuwano H,Kato H

PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors.
Proceedings of the National Academy of Sciences of the United States of America111(23)E2404-13(2014 Jun)
Ohki R,Saito K,Chen Y,Kawase T,Hiraoka N,Saigawa R,Minegishi M,Aita Y,Yanai G,Shimizu H,Yachida S,Sakata N,Doi R,Kosuge T,Shimada K,Tycko B,Tsukada T,Kanai Y,Sumi S,Namiki H,Taya Y,Shibata T,Nakagama H

Expressional and mutational analysis of PHLDA3 gene in common human cancers.
Pathology43(5)510-1(2011 Aug)
Yoo NJ,Kim YR,Lee SH

PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.
Cell136(3)535-50(2009 Feb)
Kawase T,Ohki R,Shibata T,Tsutsumi S,Kamimura N,Inazawa J,Ohta T,Ichikawa H,Aburatani H,Tashiro F,Taya Y

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PHLDA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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