CLCF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCF1
Entrez Gene ID	23529
Full Name	cardiotrophin like cytokine factor 1
Synonyms	BSF-3,BSF3,CISS2,CLC,NNT-1,NNT1,NR6
General protein information	Preferred Names cardiotrophin like cytokine factor 1 Names cardiotrophin-like cytokine factor 1 B-cell stimulatory factor 3 B-cell-stimulating factor 3 CRLF1 associated cytokine-like factor 1 neurotrophin-1/B-cell stimulating factor-3 novel neurotrophin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009].
Disorder MIM:	607672
Disorder Html:	Cold-induced sweating syndrome 2, 610313 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_013246.3	NP_037378.1	cardiotrophin-like cytokine factor 1 isoform 1 precursor
NM_013246.2	NP_037378.1	cardiotrophin-like cytokine factor 1 isoform 1 precursor
NM_001166212.1	NP_001159684.1	cardiotrophin-like cytokine factor 1 isoform 2 precursor
NM_001166212.2	NP_001159684.1	cardiotrophin-like cytokine factor 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Clcf1	NP_997498.2
Danio rerio (zebrafish)	LOC562247	XP_690733.5
Xenopus tropicalis (tropical clawed frog)	clcf1	NP_001016067.1
Bos taurus (cattle)	CLCF1	XP_002699410.1
	CLCF1	XP_001106809.1
Gallus gallus (chicken)	CLCF1	XP_003643835.2
Homo sapiens (human)	CLCF1	NP_037378.1
Canis lupus familiaris (dog)	CLCF1	XP_540818.2
Mus musculus (house mouse)	Clcf1	NP_064336.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.
Larsen JV, Kristensen AM, Pallesen LT, Bauer J, V?gter CB, Nielsen MS, Madsen P, Petersen CM
Molecular and cellular biology36(8)1272-86(2016 Apr)

Janus kinase 2/signal transducer and activator of transcription 3 inhibitors attenuate the effect of cardiotrophin-like cytokine factor 1 and human focal segmental glomerulosclerosis serum on glomerular filtration barrier.
Sharma M, Zhou J, Gauchat JF, Sharma R, McCarthy ET, Srivastava T, Savin VJ
Translational research : the journal of laboratory and clinical medicine166(4)384-98(2015 Oct)

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Sims NA
Cytokine & growth factor reviews26(5)517-22(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CLF-1, based on its binding site for CLC and on two additional and independent sites for CNTFRalpha and sorLA, is a key player in CLC and CNTFRalpha signaling and turnover.
Title: Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.

albuminuria in focal segmental glomerulosclerosis is related to qualitative or quantitative changes in the CLCF1-CRLF1 complex.
Title: Janus kinase 2/signal transducer and activator of transcription 3 inhibitors attenuate the effect of cardiotrophin-like cytokine factor 1 and human focal segmental glomerulosclerosis serum on glomerular filtration barrier.

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Title: Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.

Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain
Title: Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain.

Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha.
Title: Inactivation of cardiotrophin-like cytokine, a second ligand for ciliary neurotrophic factor receptor, leads to cold-induced sweating syndrome in a patient.

The following CLCF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16287	NM_013246.3 Latest version!	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19127	NM_001166212.2 Latest version!	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu16287	NM_013246.2	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19127	NM_001166212.1	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCF1 ( NM_013246.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037378.1 Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16287
Clone ID Related Accession (Same CDS sequence)	NM_013246.3 , NM_013246.2
Accession Version	NM_013246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	cardiotrophin-like cytokine factor 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM846622.1 and BC012939.1. On Nov 23, 2018 this sequence version replaced NM_013246.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Though this isoform has a predicted signal peptide at amino acids 1-27, experiments show it is retained within the cell. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012939.1, AF172854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312438.8/ ENSP00000309338.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC 
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG 
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA 
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG 
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG 
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG 
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037378.1
CDS	60..737
Translation	MDLRAGDSWGMLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF

Target ORF information:

RefSeq Version	NM_013246.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013246.3

ORF Insert Sequence:

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC 
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG 
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA 
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG 
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG 
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG 
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCF1 ( NM_013246.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037378.1 Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16287
Clone ID Related Accession (Same CDS sequence)	NM_013246.3 , NM_013246.2
Accession Version	NM_013246.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	cardiotrophin-like cytokine factor 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM846622.1 and BC012939.1. On Jul 28, 2004 this sequence version replaced NM_013246.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Though this isoform has a predicted signal peptide at amino acids 1-27, experiments show it is retained within the cell. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012939.1, AF172854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC 
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG 
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA 
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG 
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG 
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG 
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037378.1
CDS	197..874
Translation	MDLRAGDSWGMLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF

Target ORF information:

RefSeq Version	NM_013246.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013246.2

ORF Insert Sequence:

ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC 
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC 
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG 
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA 
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC 
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC 
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG 
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG 
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG 
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG 
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCF1 ( NM_001166212.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159684.1 Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19127
Clone ID Related Accession (Same CDS sequence)	NM_001166212.1 , NM_001166212.2
Accession Version	NM_001166212.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	cardiotrophin-like cytokine factor 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC393345.1, AK298052.1 and BC012939.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298052.1, BX497225.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA2145240 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC 
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC 
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC 
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC 
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC 
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG 
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159684.1
CDS	146..793
Translation	MLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF

Target ORF information:

RefSeq Version	NM_001166212.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166212.1

ORF Insert Sequence:

ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC 
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC 
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC 
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC 
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC 
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG 
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCF1 ( NM_001166212.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159684.1 Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19127
Clone ID Related Accession (Same CDS sequence)	NM_001166212.1 , NM_001166212.2
Accession Version	NM_001166212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	cardiotrophin-like cytokine factor 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC393345.1, AK298052.1 and BC012939.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001166212.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298052.1, SRR7346977.348087.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA2145240 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC 
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC 
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC 
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC 
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC 
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG 
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159684.1
CDS	146..793
Translation	MLACLCTVLWHLPAVPALNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF

Target ORF information:

RefSeq Version	NM_001166212.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cardiotrophin like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166212.2

ORF Insert Sequence:

ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG 
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC 
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC 
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC 
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC 
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC 
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT 
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG 
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG 
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.
Molecular and cellular biology36(8)1272-86(2016 Apr)
Larsen JV,Kristensen AM,Pallesen LT,Bauer J,V?gter CB,Nielsen MS,Madsen P,Petersen CM

Janus kinase 2/signal transducer and activator of transcription 3 inhibitors attenuate the effect of cardiotrophin-like cytokine factor 1 and human focal segmental glomerulosclerosis serum on glomerular filtration barrier.
Translational research : the journal of laboratory and clinical medicine166(4)384-98(2015 Oct)
Sharma M,Zhou J,Gauchat JF,Sharma R,McCarthy ET,Srivastava T,Savin VJ

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.
Cytokine & growth factor reviews26(5)517-22(2015 Oct)
Sims NA

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CLCF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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