SF3B1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SF3B1
Entrez Gene ID	23451
Full Name	splicing factor 3b subunit 1
Synonyms	Hsh155,MDS,PRP10,PRPF10,SAP155,SF3b155
General protein information	Preferred Names splicing factor 3b subunit 1 Names splicing factor 3B subunit 1 pre-mRNA processing 10 pre-mRNA splicing factor SF3b, 155 kDa subunit spliceosome-associated protein 155 splicing factor 3b, subunit 1, 155kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.1
Summary	This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	605590
Disorder Html:	Myelodysplastic syndrome, somatic, 614286 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308824.1	NP_001295753.1	splicing factor 3B subunit 1 isoform 3
NM_001005526.2	NP_001005526.1	splicing factor 3B subunit 1 isoform 2
NM_012433.4	NP_036565.2	splicing factor 3B subunit 1 isoform 1
NM_012433.3	NP_036565.2	splicing factor 3B subunit 1 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SF3B1	XP_545578.2
Mus musculus (house mouse)	Sf3b1	NP_112456.2
Drosophila melanogaster (fruit fly)	CG2807	NP_001259837.1
Arabidopsis thaliana (thale cress)	AT5G64270	NP_201232.1
Pan troglodytes (chimpanzee)	SF3B1	XP_516006.3
	SF3B1	XP_001086317.2
Rattus norvegicus (Norway rat)	Sf3b1	NP_445878.1
Gallus gallus (chicken)	SF3B1	XP_421912.3
Schizosaccharomyces pombe (fission yeast)	prp10	NP_594538.2
Oryza sativa (rice)	Os02g0147300	NP_001045883.1
Homo sapiens (human)	SF3B1	NP_036565.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000178	XP_310958.5
Magnaporthe oryzae (rice blast fungus)	MGG_00356	XP_003718725.1
Bos taurus (cattle)	SF3B1	NP_001179923.1
Danio rerio (zebrafish)	sf3b1	XP_005167843.1
Caenorhabditis elegans (roundworm)	T08A11.2	NP_497853.1
Neurospora crassa	NCU03042	XP_964047.1
Xenopus tropicalis (tropical clawed frog)	sf3b1	XP_002931569.2

Gene Ontology

The following SF3B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SF3B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26459	NM_001005526.2 Latest version!	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu66199	NM_001308824.1 Latest version!	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu26531	NM_012433.4 Latest version!	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OHu26531	NM_012433.3	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SF3B1 ( NM_001005526.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005526.1 Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26459
Clone ID Related Accession (Same CDS sequence)	NM_001005526.2
Accession Version	NM_001005526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY054349.1, AK294851.1 and BC046140.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2015 this sequence version replaced NM_001005526.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes a shorter isoform (2) which has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF086296.1, SRR1163658.136557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGCTTC 
TATTCTGCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005526.1
CDS	95..529
Translation	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGFYSAA

Target ORF information:

RefSeq Version	NM_001005526.2
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005526.2

ORF Insert Sequence:

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGCTTC 
TATTCTGCTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF3B1 ( NM_001308824.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295753.1 Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu66199
Clone ID Related Accession (Same CDS sequence)	NM_001308824.1
Accession Version	NM_001308824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY054349.1, AK294851.1 and BC046140.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046140.1, SRR1803616.169864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGTAAT 
TCTTTCCCAC TTTTCTATAA GTATTCAGAA ATTTATTTGT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295753.1
CDS	95..559
Translation	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGNSFPLFYKYSEIYLY

Target ORF information:

RefSeq Version	NM_001308824.1
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308824.1

ORF Insert Sequence:

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGTAAT 
TCTTTCCCAC TTTTCTATAA GTATTCAGAA ATTTATTTGT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF3B1 ( NM_012433.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036565.2 Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26531
Clone ID Related Accession (Same CDS sequence)	NM_012433.4 , NM_012433.3
Accession Version	NM_012433.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY054349.1, AK294851.1, BC039020.1, AF054284.1, BE168209.1 and AF070540.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2015 this sequence version replaced NM_012433.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF054284.1, SRR1803611.213429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAAACCCCTG ATCCTAAAAT GAATGCTAGG ACTTACATGG ATGTAATGCG AGAACAACAC 
TTGACTAAAG AAGAACGAGA AATTAGGCAA CAGCTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCGTCC CAGCCTCCAT CAAAACGAAA ACGGCGTTGG 
GATCAAACAG CTGATCAGAC TCCTGGTGCC ACTCCCAAAA AACTATCAAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC TCCTTCCTTA AGATGGGATG AGACACCAGG TCGTGCAAAG 
GGAAGCGAGA CTCCTGGAGC AACCCCAGGC TCAAAAATAT GGGATCCTAC ACCTAGCCAC 
ACACCAGCGG GAGCTGCTAC TCCTGGACGA GGTGATACAC CAGGCCATGC GACACCAGGC 
CATGGAGGCG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAAACCCC CAAAACAGAG 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GATTCTATTG GTGAAACACC GACTCCTGGA GCCAGTAAAA GAAAATCACG GTGGGATGAA 
ACACCAGCTA GTCAGATGGG TGGAAGCACT CCAGTTCTGA CCCCTGGAAA GACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCT ACTCCAGGTC ACATAATGAG TATGACTCCT 
GAACAGCTTC AGGCTTGGCG GTGGGAAAGA GAAATTGATG AGAGAAATCG CCCACTTTCT 
GATGAGGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCTCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCTA CTCCAACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAT CGAACTATGA AAAGTGTTAA TGACCAGCCA 
TCTGGAAATC TTCCATTTTT AAAACCTGAT GATATTCAAT ACTTTGATAA ACTATTGGTT 
GATGTTGATG AATCAACACT TAGTCCAGAA GAGCAAAAAG AGAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG AACACCACCA ATGAGAAAGG CTGCATTGCG TCAGATTACT 
GATAAAGCTC GTGAATTTGG AGCTGGTCCT TTGTTTAATC AGATTCTTCC TCTGCTGATG 
TCTCCTACAC TTGAGGATCA AGAGCGTCAT TTACTTGTGA AAGTTATTGA TAGGATACTG 
TACAAACTTG ATGACTTAGT TCGTCCATAT GTGCATAAGA TCCTCGTGGT CATTGAACCG 
CTATTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATCAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATC TCTACCATGA GACCTGATAT AGATAACATG 
GATGAGTATG TCCGTAACAC AACAGCTAGA GCTTTTGCTG TTGTAGCCTC TGCCCTGGGC 
ATTCCTTCTT TATTGCCCTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC CTGGCAAGCG 
AGACACACTG GTATTAAGAT TGTACAACAG ATAGCTATTC TTATGGGCTG TGCCATCTTG 
CCACATCTTA GAAGTTTAGT TGAAATCATT GAACATGGTC TTGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTTT GGCCATTGCT GCCTTGGCTG AAGCAGCAAC TCCTTATGGT 
ATCGAATCTT TTGATTCTGT GTTAAAGCCT TTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTTTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTTA TTCGAGAATT CCAGTCTCCT 
GATGAGGAAA TGAAAAAAAT TGTGCTGAAG GTGGTAAAAC AGTGTTGTGG GACAGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCTCCCT TTTTTAAACA CTTCTGGCAG 
CACAGGATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACTAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAAATTATA TCCAGGATTG TGGATGATCT GAAAGATGAA 
GCCGAACAGT ACAGAAAAAT GGTGATGGAG ACAATTGAGA AAATTATGGG TAATTTGGGA 
GCAGCAGATA TTGATCATAA ACTTGAAGAA CAACTGATTG ATGGTATTCT TTATGCTTTC 
CAAGAACAGA CTACAGAGGA CTCAGTAATG TTGAACGGCT TTGGCACAGT GGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT GTGGCGTTTA 
AATAACAAAT CTGCTAAAGT TAGGCAACAG GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTCATGAAGA CTTGTCAAGA GGAAAAATTG ATGGGACACT TGGGTGTTGT ATTGTATGAG 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCCATT 
GTAAATGTCA TAGGTATGCA TAAGATGACT CCACCAATTA AAGATCTGCT GCCTAGACTC 
ACCCCCATCT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGT 
CGTATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AGTGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCCCACAAA AAGGCTATTC GTAGAGCCAC AGTCAACACA 
TTTGGTTATA TTGCAAAGGC CATTGGCCCT CATGATGTAT TGGCTACACT TCTGAACAAC 
CTCAAAGTTC AAGAAAGGCA GAACAGAGTT TGTACCACTG TAGCAATAGC TATTGTTGCA 
GAAACATGTT CACCCTTTAC AGTACTCCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTGAATGTTC AAAATGGAGT GTTAAAATCG CTTTCCTTCT TGTTTGAATA TATTGGTGAA 
ATGGGAAAAG ACTACATTTA TGCCGTAACA CCGTTACTTG AAGATGCTTT AATGGATAGA 
GACCTTGTAC ACAGACAGAC GGCTAGTGCA GTGGTACAGC ACATGTCACT TGGGGTTTAT 
GGATTTGGTT GTGAAGATTC GCTGAATCAC TTGTTGAACT ATGTATGGCC CAATGTATTT 
GAGACATCTC CTCATGTAAT TCAGGCAGTT ATGGGAGCCC TAGAGGGCCT GAGAGTTGCT 
ATTGGACCAT GTAGAATGTT GCAATATTGT TTACAGGGTC TGTTTCACCC AGCCCGGAAA 
GTCAGAGATG TATATTGGAA AATTTACAAC TCCATCTACA TTGGTTCCCA GGACGCTCTC 
ATAGCACATT ACCCAAGAAT CTACAACGAT GATAAGAACA CCTATATTCG TTATGAACTT 
GACTATATCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036565.2
CDS	95..4009
Translation	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	NM_012433.3
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012433.3

ORF Insert Sequence:

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAAACCCCTG ATCCTAAAAT GAATGCTAGG ACTTACATGG ATGTAATGCG AGAACAACAC 
TTGACTAAAG AAGAACGAGA AATTAGGCAA CAGCTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCGTCC CAGCCTCCAT CAAAACGAAA ACGGCGTTGG 
GATCAAACAG CTGATCAGAC TCCTGGTGCC ACTCCCAAAA AACTATCAAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC TCCTTCCTTA AGATGGGATG AGACACCAGG TCGTGCAAAG 
GGAAGCGAGA CTCCTGGAGC AACCCCAGGC TCAAAAATAT GGGATCCTAC ACCTAGCCAC 
ACACCAGCGG GAGCTGCTAC TCCTGGACGA GGTGATACAC CAGGCCATGC GACACCAGGC 
CATGGAGGCG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAAACCCC CAAAACAGAG 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GATTCTATTG GTGAAACACC GACTCCTGGA GCCAGTAAAA GAAAATCACG GTGGGATGAA 
ACACCAGCTA GTCAGATGGG TGGAAGCACT CCAGTTCTGA CCCCTGGAAA GACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCT ACTCCAGGTC ACATAATGAG TATGACTCCT 
GAACAGCTTC AGGCTTGGCG GTGGGAAAGA GAAATTGATG AGAGAAATCG CCCACTTTCT 
GATGAGGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCTCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCTA CTCCAACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAT CGAACTATGA AAAGTGTTAA TGACCAGCCA 
TCTGGAAATC TTCCATTTTT AAAACCTGAT GATATTCAAT ACTTTGATAA ACTATTGGTT 
GATGTTGATG AATCAACACT TAGTCCAGAA GAGCAAAAAG AGAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG AACACCACCA ATGAGAAAGG CTGCATTGCG TCAGATTACT 
GATAAAGCTC GTGAATTTGG AGCTGGTCCT TTGTTTAATC AGATTCTTCC TCTGCTGATG 
TCTCCTACAC TTGAGGATCA AGAGCGTCAT TTACTTGTGA AAGTTATTGA TAGGATACTG 
TACAAACTTG ATGACTTAGT TCGTCCATAT GTGCATAAGA TCCTCGTGGT CATTGAACCG 
CTATTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATCAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATC TCTACCATGA GACCTGATAT AGATAACATG 
GATGAGTATG TCCGTAACAC AACAGCTAGA GCTTTTGCTG TTGTAGCCTC TGCCCTGGGC 
ATTCCTTCTT TATTGCCCTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC CTGGCAAGCG 
AGACACACTG GTATTAAGAT TGTACAACAG ATAGCTATTC TTATGGGCTG TGCCATCTTG 
CCACATCTTA GAAGTTTAGT TGAAATCATT GAACATGGTC TTGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTTT GGCCATTGCT GCCTTGGCTG AAGCAGCAAC TCCTTATGGT 
ATCGAATCTT TTGATTCTGT GTTAAAGCCT TTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTTTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTTA TTCGAGAATT CCAGTCTCCT 
GATGAGGAAA TGAAAAAAAT TGTGCTGAAG GTGGTAAAAC AGTGTTGTGG GACAGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCTCCCT TTTTTAAACA CTTCTGGCAG 
CACAGGATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACTAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAAATTATA TCCAGGATTG TGGATGATCT GAAAGATGAA 
GCCGAACAGT ACAGAAAAAT GGTGATGGAG ACAATTGAGA AAATTATGGG TAATTTGGGA 
GCAGCAGATA TTGATCATAA ACTTGAAGAA CAACTGATTG ATGGTATTCT TTATGCTTTC 
CAAGAACAGA CTACAGAGGA CTCAGTAATG TTGAACGGCT TTGGCACAGT GGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT GTGGCGTTTA 
AATAACAAAT CTGCTAAAGT TAGGCAACAG GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTCATGAAGA CTTGTCAAGA GGAAAAATTG ATGGGACACT TGGGTGTTGT ATTGTATGAG 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCCATT 
GTAAATGTCA TAGGTATGCA TAAGATGACT CCACCAATTA AAGATCTGCT GCCTAGACTC 
ACCCCCATCT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGT 
CGTATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AGTGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCCCACAAA AAGGCTATTC GTAGAGCCAC AGTCAACACA 
TTTGGTTATA TTGCAAAGGC CATTGGCCCT CATGATGTAT TGGCTACACT TCTGAACAAC 
CTCAAAGTTC AAGAAAGGCA GAACAGAGTT TGTACCACTG TAGCAATAGC TATTGTTGCA 
GAAACATGTT CACCCTTTAC AGTACTCCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTGAATGTTC AAAATGGAGT GTTAAAATCG CTTTCCTTCT TGTTTGAATA TATTGGTGAA 
ATGGGAAAAG ACTACATTTA TGCCGTAACA CCGTTACTTG AAGATGCTTT AATGGATAGA 
GACCTTGTAC ACAGACAGAC GGCTAGTGCA GTGGTACAGC ACATGTCACT TGGGGTTTAT 
GGATTTGGTT GTGAAGATTC GCTGAATCAC TTGTTGAACT ATGTATGGCC CAATGTATTT 
GAGACATCTC CTCATGTAAT TCAGGCAGTT ATGGGAGCCC TAGAGGGCCT GAGAGTTGCT 
ATTGGACCAT GTAGAATGTT GCAATATTGT TTACAGGGTC TGTTTCACCC AGCCCGGAAA 
GTCAGAGATG TATATTGGAA AATTTACAAC TCCATCTACA TTGGTTCCCA GGACGCTCTC 
ATAGCACATT ACCCAAGAAT CTACAACGAT GATAAGAACA CCTATATTCG TTATGAACTT 
GACTATATCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF3B1 ( NM_012433.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036565.2 Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.

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CloneID	OHu26531
Clone ID Related Accession (Same CDS sequence)	NM_012433.4 , NM_012433.3
Accession Version	NM_012433.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	splicing factor 3B subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294851.1, BC039020.1, AF054284.1, BE168209.1, AF070540.1 and AC010746.8. On Nov 21, 2019 this sequence version replaced NM_012433.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.250476.1, AF054284.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAAACCCCTG ATCCTAAAAT GAATGCTAGG ACTTACATGG ATGTAATGCG AGAACAACAC 
TTGACTAAAG AAGAACGAGA AATTAGGCAA CAGCTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCGTCC CAGCCTCCAT CAAAACGAAA ACGGCGTTGG 
GATCAAACAG CTGATCAGAC TCCTGGTGCC ACTCCCAAAA AACTATCAAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC TCCTTCCTTA AGATGGGATG AGACACCAGG TCGTGCAAAG 
GGAAGCGAGA CTCCTGGAGC AACCCCAGGC TCAAAAATAT GGGATCCTAC ACCTAGCCAC 
ACACCAGCGG GAGCTGCTAC TCCTGGACGA GGTGATACAC CAGGCCATGC GACACCAGGC 
CATGGAGGCG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAAACCCC CAAAACAGAG 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GATTCTATTG GTGAAACACC GACTCCTGGA GCCAGTAAAA GAAAATCACG GTGGGATGAA 
ACACCAGCTA GTCAGATGGG TGGAAGCACT CCAGTTCTGA CCCCTGGAAA GACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCT ACTCCAGGTC ACATAATGAG TATGACTCCT 
GAACAGCTTC AGGCTTGGCG GTGGGAAAGA GAAATTGATG AGAGAAATCG CCCACTTTCT 
GATGAGGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCTCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCTA CTCCAACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAT CGAACTATGA AAAGTGTTAA TGACCAGCCA 
TCTGGAAATC TTCCATTTTT AAAACCTGAT GATATTCAAT ACTTTGATAA ACTATTGGTT 
GATGTTGATG AATCAACACT TAGTCCAGAA GAGCAAAAAG AGAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG AACACCACCA ATGAGAAAGG CTGCATTGCG TCAGATTACT 
GATAAAGCTC GTGAATTTGG AGCTGGTCCT TTGTTTAATC AGATTCTTCC TCTGCTGATG 
TCTCCTACAC TTGAGGATCA AGAGCGTCAT TTACTTGTGA AAGTTATTGA TAGGATACTG 
TACAAACTTG ATGACTTAGT TCGTCCATAT GTGCATAAGA TCCTCGTGGT CATTGAACCG 
CTATTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATCAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATC TCTACCATGA GACCTGATAT AGATAACATG 
GATGAGTATG TCCGTAACAC AACAGCTAGA GCTTTTGCTG TTGTAGCCTC TGCCCTGGGC 
ATTCCTTCTT TATTGCCCTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC CTGGCAAGCG 
AGACACACTG GTATTAAGAT TGTACAACAG ATAGCTATTC TTATGGGCTG TGCCATCTTG 
CCACATCTTA GAAGTTTAGT TGAAATCATT GAACATGGTC TTGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTTT GGCCATTGCT GCCTTGGCTG AAGCAGCAAC TCCTTATGGT 
ATCGAATCTT TTGATTCTGT GTTAAAGCCT TTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTTTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTTA TTCGAGAATT CCAGTCTCCT 
GATGAGGAAA TGAAAAAAAT TGTGCTGAAG GTGGTAAAAC AGTGTTGTGG GACAGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCTCCCT TTTTTAAACA CTTCTGGCAG 
CACAGGATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACTAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAAATTATA TCCAGGATTG TGGATGATCT GAAAGATGAA 
GCCGAACAGT ACAGAAAAAT GGTGATGGAG ACAATTGAGA AAATTATGGG TAATTTGGGA 
GCAGCAGATA TTGATCATAA ACTTGAAGAA CAACTGATTG ATGGTATTCT TTATGCTTTC 
CAAGAACAGA CTACAGAGGA CTCAGTAATG TTGAACGGCT TTGGCACAGT GGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT GTGGCGTTTA 
AATAACAAAT CTGCTAAAGT TAGGCAACAG GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTCATGAAGA CTTGTCAAGA GGAAAAATTG ATGGGACACT TGGGTGTTGT ATTGTATGAG 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCCATT 
GTAAATGTCA TAGGTATGCA TAAGATGACT CCACCAATTA AAGATCTGCT GCCTAGACTC 
ACCCCCATCT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGT 
CGTATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AGTGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCCCACAAA AAGGCTATTC GTAGAGCCAC AGTCAACACA 
TTTGGTTATA TTGCAAAGGC CATTGGCCCT CATGATGTAT TGGCTACACT TCTGAACAAC 
CTCAAAGTTC AAGAAAGGCA GAACAGAGTT TGTACCACTG TAGCAATAGC TATTGTTGCA 
GAAACATGTT CACCCTTTAC AGTACTCCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTGAATGTTC AAAATGGAGT GTTAAAATCG CTTTCCTTCT TGTTTGAATA TATTGGTGAA 
ATGGGAAAAG ACTACATTTA TGCCGTAACA CCGTTACTTG AAGATGCTTT AATGGATAGA 
GACCTTGTAC ACAGACAGAC GGCTAGTGCA GTGGTACAGC ACATGTCACT TGGGGTTTAT 
GGATTTGGTT GTGAAGATTC GCTGAATCAC TTGTTGAACT ATGTATGGCC CAATGTATTT 
GAGACATCTC CTCATGTAAT TCAGGCAGTT ATGGGAGCCC TAGAGGGCCT GAGAGTTGCT 
ATTGGACCAT GTAGAATGTT GCAATATTGT TTACAGGGTC TGTTTCACCC AGCCCGGAAA 
GTCAGAGATG TATATTGGAA AATTTACAAC TCCATCTACA TTGGTTCCCA GGACGCTCTC 
ATAGCACATT ACCCAAGAAT CTACAACGAT GATAAGAACA CCTATATTCG TTATGAACTT 
GACTATATCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036565.2
CDS	30..3944
Translation	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Target ORF information:

RefSeq Version	NM_012433.4
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3b subunit 1 (SF3B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012433.4

ORF Insert Sequence:

ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATTGAAGCAC AGATTCGAGA AATTCAAGGC 
AAGAAGGCAG CTCTTGATGA AGCTCAAGGA GTGGGCCTCG ATTCTACAGG TTATTATGAC 
CAGGAAATTT ATGGTGGAAG TGACAGCAGA TTTGCTGGAT ACGTGACATC AATTGCTGCA 
ACTGAACTTG AAGATGATGA CGATGACTAT TCATCATCTA CGAGTTTGCT TGGTCAGAAG 
AAGCCAGGAT ATCATGCCCC TGTGGCATTG CTTAATGATA TACCACAGTC AACAGAACAG 
TATGATCCAT TTGCTGAGCA CAGACCTCCA AAGATTGCAG ACCGGGAAGA TGAATACAAA 
AAGCATAGGC GGACCATGAT AATTTCCCCA GAGCGTCTTG ATCCTTTTGC AGATGGAGGG 
AAAACCCCTG ATCCTAAAAT GAATGCTAGG ACTTACATGG ATGTAATGCG AGAACAACAC 
TTGACTAAAG AAGAACGAGA AATTAGGCAA CAGCTAGCAG AAAAAGCTAA AGCTGGAGAA 
CTAAAAGTCG TCAATGGAGC AGCAGCGTCC CAGCCTCCAT CAAAACGAAA ACGGCGTTGG 
GATCAAACAG CTGATCAGAC TCCTGGTGCC ACTCCCAAAA AACTATCAAG TTGGGATCAG 
GCAGAGACCC CTGGGCATAC TCCTTCCTTA AGATGGGATG AGACACCAGG TCGTGCAAAG 
GGAAGCGAGA CTCCTGGAGC AACCCCAGGC TCAAAAATAT GGGATCCTAC ACCTAGCCAC 
ACACCAGCGG GAGCTGCTAC TCCTGGACGA GGTGATACAC CAGGCCATGC GACACCAGGC 
CATGGAGGCG CAACTTCCAG TGCTCGTAAA AACAGATGGG ATGAAACCCC CAAAACAGAG 
AGAGATACTC CTGGGCATGG AAGTGGATGG GCTGAGACTC CTCGAACAGA TCGAGGTGGA 
GATTCTATTG GTGAAACACC GACTCCTGGA GCCAGTAAAA GAAAATCACG GTGGGATGAA 
ACACCAGCTA GTCAGATGGG TGGAAGCACT CCAGTTCTGA CCCCTGGAAA GACACCAATT 
GGCACACCAG CCATGAACAT GGCTACCCCT ACTCCAGGTC ACATAATGAG TATGACTCCT 
GAACAGCTTC AGGCTTGGCG GTGGGAAAGA GAAATTGATG AGAGAAATCG CCCACTTTCT 
GATGAGGAAT TAGATGCTAT GTTCCCAGAA GGATATAAGG TACTTCCTCC TCCAGCTGGT 
TATGTTCCTA TTCGAACTCC AGCTCGAAAG CTGACAGCTA CTCCAACACC TTTGGGTGGT 
ATGACTGGTT TCCACATGCA AACTGAAGAT CGAACTATGA AAAGTGTTAA TGACCAGCCA 
TCTGGAAATC TTCCATTTTT AAAACCTGAT GATATTCAAT ACTTTGATAA ACTATTGGTT 
GATGTTGATG AATCAACACT TAGTCCAGAA GAGCAAAAAG AGAGAAAAAT AATGAAGTTG 
CTTTTAAAAA TTAAGAATGG AACACCACCA ATGAGAAAGG CTGCATTGCG TCAGATTACT 
GATAAAGCTC GTGAATTTGG AGCTGGTCCT TTGTTTAATC AGATTCTTCC TCTGCTGATG 
TCTCCTACAC TTGAGGATCA AGAGCGTCAT TTACTTGTGA AAGTTATTGA TAGGATACTG 
TACAAACTTG ATGACTTAGT TCGTCCATAT GTGCATAAGA TCCTCGTGGT CATTGAACCG 
CTATTGATTG ATGAAGATTA CTATGCTAGA GTGGAAGGCC GAGAGATCAT TTCTAATTTG 
GCAAAGGCTG CTGGTCTGGC TACTATGATC TCTACCATGA GACCTGATAT AGATAACATG 
GATGAGTATG TCCGTAACAC AACAGCTAGA GCTTTTGCTG TTGTAGCCTC TGCCCTGGGC 
ATTCCTTCTT TATTGCCCTT CTTAAAAGCT GTGTGCAAAA GCAAGAAGTC CTGGCAAGCG 
AGACACACTG GTATTAAGAT TGTACAACAG ATAGCTATTC TTATGGGCTG TGCCATCTTG 
CCACATCTTA GAAGTTTAGT TGAAATCATT GAACATGGTC TTGTGGATGA GCAGCAGAAA 
GTTCGGACCA TCAGTGCTTT GGCCATTGCT GCCTTGGCTG AAGCAGCAAC TCCTTATGGT 
ATCGAATCTT TTGATTCTGT GTTAAAGCCT TTATGGAAGG GTATCCGCCA ACACAGAGGA 
AAGGGTTTGG CTGCTTTCTT GAAGGCTATT GGGTATCTTA TTCCTCTTAT GGATGCAGAA 
TATGCCAACT ACTATACTAG AGAAGTGATG TTAATCCTTA TTCGAGAATT CCAGTCTCCT 
GATGAGGAAA TGAAAAAAAT TGTGCTGAAG GTGGTAAAAC AGTGTTGTGG GACAGATGGT 
GTAGAAGCAA ACTACATTAA AACAGAGATT CTTCCTCCCT TTTTTAAACA CTTCTGGCAG 
CACAGGATGG CTTTGGATAG AAGAAATTAC CGACAGTTAG TTGATACTAC TGTGGAGTTG 
GCAAACAAAG TAGGTGCAGC AGAAATTATA TCCAGGATTG TGGATGATCT GAAAGATGAA 
GCCGAACAGT ACAGAAAAAT GGTGATGGAG ACAATTGAGA AAATTATGGG TAATTTGGGA 
GCAGCAGATA TTGATCATAA ACTTGAAGAA CAACTGATTG ATGGTATTCT TTATGCTTTC 
CAAGAACAGA CTACAGAGGA CTCAGTAATG TTGAACGGCT TTGGCACAGT GGTTAATGCT 
CTTGGCAAAC GAGTCAAACC ATACTTGCCT CAGATCTGTG GTACAGTTTT GTGGCGTTTA 
AATAACAAAT CTGCTAAAGT TAGGCAACAG GCAGCTGACT TGATTTCTCG AACTGCTGTT 
GTCATGAAGA CTTGTCAAGA GGAAAAATTG ATGGGACACT TGGGTGTTGT ATTGTATGAG 
TATTTGGGTG AAGAGTACCC TGAAGTATTG GGCAGCATTC TTGGAGCACT GAAGGCCATT 
GTAAATGTCA TAGGTATGCA TAAGATGACT CCACCAATTA AAGATCTGCT GCCTAGACTC 
ACCCCCATCT TAAAGAACAG ACATGAAAAA GTACAAGAGA ATTGTATTGA TCTTGTTGGT 
CGTATTGCTG ACAGGGGAGC TGAATATGTA TCTGCAAGAG AGTGGATGAG GATTTGCTTT 
GAGCTTTTAG AGCTCTTAAA AGCCCACAAA AAGGCTATTC GTAGAGCCAC AGTCAACACA 
TTTGGTTATA TTGCAAAGGC CATTGGCCCT CATGATGTAT TGGCTACACT TCTGAACAAC 
CTCAAAGTTC AAGAAAGGCA GAACAGAGTT TGTACCACTG TAGCAATAGC TATTGTTGCA 
GAAACATGTT CACCCTTTAC AGTACTCCCT GCCTTAATGA ATGAATACAG AGTTCCTGAA 
CTGAATGTTC AAAATGGAGT GTTAAAATCG CTTTCCTTCT TGTTTGAATA TATTGGTGAA 
ATGGGAAAAG ACTACATTTA TGCCGTAACA CCGTTACTTG AAGATGCTTT AATGGATAGA 
GACCTTGTAC ACAGACAGAC GGCTAGTGCA GTGGTACAGC ACATGTCACT TGGGGTTTAT 
GGATTTGGTT GTGAAGATTC GCTGAATCAC TTGTTGAACT ATGTATGGCC CAATGTATTT 
GAGACATCTC CTCATGTAAT TCAGGCAGTT ATGGGAGCCC TAGAGGGCCT GAGAGTTGCT 
ATTGGACCAT GTAGAATGTT GCAATATTGT TTACAGGGTC TGTTTCACCC AGCCCGGAAA 
GTCAGAGATG TATATTGGAA AATTTACAAC TCCATCTACA TTGGTTCCCA GGACGCTCTC 
ATAGCACATT ACCCAAGAAT CTACAACGAT GATAAGAACA CCTATATTCG TTATGAACTT 
GACTATATCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SF3B1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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