HARS2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_012208.3	NP_036340.1	probable histidine--tRNA ligase, mitochondrial isoform 1 precursor
XM_017009287.1	XP_016864776.1	probable histidine--tRNA ligase, mitochondrial isoform X3
XM_011537619.1	XP_011535921.1	probable histidine--tRNA ligase, mitochondrial isoform X1
NM_001278732.2	NP_001265661.1	probable histidine--tRNA ligase, mitochondrial isoform 3
XM_017009289.1	XP_016864778.1	probable histidine--tRNA ligase, mitochondrial isoform X2
NM_001278731.1	NP_001265660.1	probable histidine--tRNA ligase, mitochondrial isoform 2 precursor
NM_001363535.1	NP_001350464.1	probable histidine--tRNA ligase, mitochondrial isoform 4 precursor
NM_001363536.2	NP_001350465.1	probable histidine--tRNA ligase, mitochondrial isoform 5
XM_017009291.1	XP_016864780.1	probable histidine--tRNA ligase, mitochondrial isoform X3
XM_017009292.1	XP_016864781.1	probable histidine--tRNA ligase, mitochondrial isoform X3
XM_017009288.1	XP_016864777.1	probable histidine--tRNA ligase, mitochondrial isoform X2
XM_017009290.1	XP_016864779.1	probable histidine--tRNA ligase, mitochondrial isoform X5
NM_001363535.2	NP_001350464.1	probable histidine--tRNA ligase, mitochondrial isoform 4 precursor
XM_017009290.2	XP_016864779.1	probable histidine--tRNA ligase, mitochondrial isoform X3
NM_001363536.1	NP_001350465.1	probable histidine--tRNA ligase, mitochondrial isoform 5
XM_011537620.1	XP_011535922.1	probable histidine--tRNA ligase, mitochondrial isoform X2
NM_001278732.1	NP_001265661.1	probable histidine--tRNA ligase, mitochondrial isoform 3
NM_012208.4	NP_036340.1	probable histidine--tRNA ligase, mitochondrial isoform 1 precursor
NM_001278731.2	NP_001265660.1	probable histidine--tRNA ligase, mitochondrial isoform 2 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21569	NM_012208.4 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu09743	NM_001278732.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09767	NM_001278731.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu66192	NM_001363535.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu95786	NM_001363536.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu66192	XM_011537619.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu66193	XM_011537620.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu95786	XM_017009288.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu95786	XM_017009289.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu95787	XM_017009290.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu95787	XM_017009292.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu95787	XM_017009291.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu95785	XM_017009287.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu66192	NM_001363535.1	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu95787	XM_017009290.1	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu95786	NM_001363536.1	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09743	NM_001278732.1	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu21569	NM_012208.3	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu09767	NM_001278731.1	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

HARS2 ( NM_012208.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036340.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21569
Clone ID Related Accession (Same CDS sequence)	NM_012208.3 , NM_012208.4
Accession Version	NM_012208.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1, BC014982.1 and AI865702.1. This sequence is a reference standard in the RefSeqGene project. On Jul 13, 2013 this sequence version replaced NM_012208.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803612.173886.1, SRR1803614.250150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGGTAAATG ACCGGCGGAT TGTGGATGGG 
ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036340.1
CDS	224..1744
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_012208.3
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012208.3

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGGTAAATG ACCGGCGGAT TGTGGATGGG 
ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_012208.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036340.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21569
Clone ID Related Accession (Same CDS sequence)	NM_012208.3 , NM_012208.4
Accession Version	NM_012208.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1 and BC014982.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_012208.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.11907.1, SRR1803614.250150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000230771.9/ ENSP00000230771.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGGTAAATG ACCGGCGGAT TGTGGATGGG 
ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036340.1
CDS	195..1715
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_012208.4
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012208.4

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGGTAAATG ACCGGCGGAT TGTGGATGGG 
ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001278732.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265661.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09743
Clone ID Related Accession (Same CDS sequence)	NM_001278732.2 , NM_001278732.1
Accession Version	NM_001278732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1, BC014982.1, AK293269.1 and BQ228600.1. On May 31, 2019 this sequence version replaced NM_001278732.1. Transcript Variant: This variant (3) uses an alternate splice site and lacks two alternate in-frame exons in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. This isoform (3) lacks the transit peptide present in isoforms 1 and 2, hence it is not likely to localize to the mitochondrion. ##Evidence-Data-START## Transcript exon combination :: AK293269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGATTTTG ACATTGCTGG TCAGTTTGAC 
CCTATGATCC CCGATGCAGA GTGTTTGAAG ATCATGTGTG AAATCCTAAG TGGATTGCAG 
TTGGGAGACT TTCTCATTAA GGTAAATGAC CGGCGGATTG TGGATGGGAT GTTTGCTGTC 
TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC ATCTGCTCCT CCATAGATAA ACTAGACAAG 
ATGGCTTGGA AAGATGTGAG ACATGAGATG GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG 
GCTGATCGAA TTGGGGACTA TGTCCAGTGT CATGGTGGGG TATCCCTAGT AGAGCAAATG 
TTTCAGGATC CCAGACTATC CCAGAACAAG CAGGCCCTGG AGGGCCTGGG AGACCTAAAG 
CTGCTATTTG AATACCTGAC TTTATTTGGA ATTGCTGATA AGATCTCCTT TGACCTCAGC 
CTGGCTCGGG GCCTAGACTA CTATACAGGA GTGATCTATG AAGCAGTGCT GCTGCAGACC 
CCAACTCAGG CTGGGGAGGA GCCCCTGAAT GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT 
GATGGGCTGG TGGGCATGTT TGACCCCAAG GGCCACAAGG TGCCATGTGT GGGACTCAGC 
ATTGGGGTTG AGCGAATCTT CTACATTGTG GAGCAGAGGA TGAAGACCAA AGGTGAGAAG 
GTGCGGACTA CAGAGACTCA AGTGTTTGTG GCCACACCAC AGAAGAACTT TCTCCAAGAA 
CGGTTGAAGC TTATTGCAGA GCTTTGGGAT TCTGGAATCA AGGCAGAGAT GCTATACAAG 
AACAACCCCA AACTATTAAC CCAGCTGCAC TATTGTGAGA GCACAGGCAT TCCACTGGTG 
GTCATTATTG GTGAGCAAGA ACTGAAAGAA GGGGTCATCA AGATCCGTTC AGTGGCCAGC 
AGAGAGGAGG TGGCCATTAA ACGGGAAAAT TTTGTGGCTG AAATTCAGAA GCGACTGTCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265661.1
CDS	505..1593
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001278732.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278732.2

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGATTTTG ACATTGCTGG TCAGTTTGAC 
CCTATGATCC CCGATGCAGA GTGTTTGAAG ATCATGTGTG AAATCCTAAG TGGATTGCAG 
TTGGGAGACT TTCTCATTAA GGTAAATGAC CGGCGGATTG TGGATGGGAT GTTTGCTGTC 
TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC ATCTGCTCCT CCATAGATAA ACTAGACAAG 
ATGGCTTGGA AAGATGTGAG ACATGAGATG GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG 
GCTGATCGAA TTGGGGACTA TGTCCAGTGT CATGGTGGGG TATCCCTAGT AGAGCAAATG 
TTTCAGGATC CCAGACTATC CCAGAACAAG CAGGCCCTGG AGGGCCTGGG AGACCTAAAG 
CTGCTATTTG AATACCTGAC TTTATTTGGA ATTGCTGATA AGATCTCCTT TGACCTCAGC 
CTGGCTCGGG GCCTAGACTA CTATACAGGA GTGATCTATG AAGCAGTGCT GCTGCAGACC 
CCAACTCAGG CTGGGGAGGA GCCCCTGAAT GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT 
GATGGGCTGG TGGGCATGTT TGACCCCAAG GGCCACAAGG TGCCATGTGT GGGACTCAGC 
ATTGGGGTTG AGCGAATCTT CTACATTGTG GAGCAGAGGA TGAAGACCAA AGGTGAGAAG 
GTGCGGACTA CAGAGACTCA AGTGTTTGTG GCCACACCAC AGAAGAACTT TCTCCAAGAA 
CGGTTGAAGC TTATTGCAGA GCTTTGGGAT TCTGGAATCA AGGCAGAGAT GCTATACAAG 
AACAACCCCA AACTATTAAC CCAGCTGCAC TATTGTGAGA GCACAGGCAT TCCACTGGTG 
GTCATTATTG GTGAGCAAGA ACTGAAAGAA GGGGTCATCA AGATCCGTTC AGTGGCCAGC 
AGAGAGGAGG TGGCCATTAA ACGGGAAAAT TTTGTGGCTG AAATTCAGAA GCGACTGTCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001278732.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265661.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.

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CloneID	OHu09743
Clone ID Related Accession (Same CDS sequence)	NM_001278732.2 , NM_001278732.1
Accession Version	NM_001278732.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1, BC014982.1, AK293269.1, BQ228600.1 and AI865702.1. Transcript Variant: This variant (3) uses an alternate splice site and lacks two alternate in-frame exons in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. This isoform (3) lacks the transit peptide present in isoforms 1 and 2, hence it is not likely to localize to the mitochondrion. ##Evidence-Data-START## Transcript exon combination :: AK293269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGATTTTG ACATTGCTGG TCAGTTTGAC 
CCTATGATCC CCGATGCAGA GTGTTTGAAG ATCATGTGTG AAATCCTAAG TGGATTGCAG 
TTGGGAGACT TTCTCATTAA GGTAAATGAC CGGCGGATTG TGGATGGGAT GTTTGCTGTC 
TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC ATCTGCTCCT CCATAGATAA ACTAGACAAG 
ATGGCTTGGA AAGATGTGAG ACATGAGATG GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG 
GCTGATCGAA TTGGGGACTA TGTCCAGTGT CATGGTGGGG TATCCCTAGT AGAGCAAATG 
TTTCAGGATC CCAGACTATC CCAGAACAAG CAGGCCCTGG AGGGCCTGGG AGACCTAAAG 
CTGCTATTTG AATACCTGAC TTTATTTGGA ATTGCTGATA AGATCTCCTT TGACCTCAGC 
CTGGCTCGGG GCCTAGACTA CTATACAGGA GTGATCTATG AAGCAGTGCT GCTGCAGACC 
CCAACTCAGG CTGGGGAGGA GCCCCTGAAT GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT 
GATGGGCTGG TGGGCATGTT TGACCCCAAG GGCCACAAGG TGCCATGTGT GGGACTCAGC 
ATTGGGGTTG AGCGAATCTT CTACATTGTG GAGCAGAGGA TGAAGACCAA AGGTGAGAAG 
GTGCGGACTA CAGAGACTCA AGTGTTTGTG GCCACACCAC AGAAGAACTT TCTCCAAGAA 
CGGTTGAAGC TTATTGCAGA GCTTTGGGAT TCTGGAATCA AGGCAGAGAT GCTATACAAG 
AACAACCCCA AACTATTAAC CCAGCTGCAC TATTGTGAGA GCACAGGCAT TCCACTGGTG 
GTCATTATTG GTGAGCAAGA ACTGAAAGAA GGGGTCATCA AGATCCGTTC AGTGGCCAGC 
AGAGAGGAGG TGGCCATTAA ACGGGAAAAT TTTGTGGCTG AAATTCAGAA GCGACTGTCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265661.1
CDS	534..1622
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001278732.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278732.1

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGATTTTG ACATTGCTGG TCAGTTTGAC 
CCTATGATCC CCGATGCAGA GTGTTTGAAG ATCATGTGTG AAATCCTAAG TGGATTGCAG 
TTGGGAGACT TTCTCATTAA GGTAAATGAC CGGCGGATTG TGGATGGGAT GTTTGCTGTC 
TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC ATCTGCTCCT CCATAGATAA ACTAGACAAG 
ATGGCTTGGA AAGATGTGAG ACATGAGATG GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG 
GCTGATCGAA TTGGGGACTA TGTCCAGTGT CATGGTGGGG TATCCCTAGT AGAGCAAATG 
TTTCAGGATC CCAGACTATC CCAGAACAAG CAGGCCCTGG AGGGCCTGGG AGACCTAAAG 
CTGCTATTTG AATACCTGAC TTTATTTGGA ATTGCTGATA AGATCTCCTT TGACCTCAGC 
CTGGCTCGGG GCCTAGACTA CTATACAGGA GTGATCTATG AAGCAGTGCT GCTGCAGACC 
CCAACTCAGG CTGGGGAGGA GCCCCTGAAT GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT 
GATGGGCTGG TGGGCATGTT TGACCCCAAG GGCCACAAGG TGCCATGTGT GGGACTCAGC 
ATTGGGGTTG AGCGAATCTT CTACATTGTG GAGCAGAGGA TGAAGACCAA AGGTGAGAAG 
GTGCGGACTA CAGAGACTCA AGTGTTTGTG GCCACACCAC AGAAGAACTT TCTCCAAGAA 
CGGTTGAAGC TTATTGCAGA GCTTTGGGAT TCTGGAATCA AGGCAGAGAT GCTATACAAG 
AACAACCCCA AACTATTAAC CCAGCTGCAC TATTGTGAGA GCACAGGCAT TCCACTGGTG 
GTCATTATTG GTGAGCAAGA ACTGAAAGAA GGGGTCATCA AGATCCGTTC AGTGGCCAGC 
AGAGAGGAGG TGGCCATTAA ACGGGAAAAT TTTGTGGCTG AAATTCAGAA GCGACTGTCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001278731.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265660.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.

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CloneID	OHu09767
Clone ID Related Accession (Same CDS sequence)	NM_001278731.1 , NM_001278731.2
Accession Version	NM_001278731.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1, BC014982.1, AK293390.1 and AI865702.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.256096.1, SRR1803616.121381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TACCAGGGAT 
CTTAGTCCTC AGCATATGGT TGTGAGGGAG AAAATTCTTG ATTTGGTTAT CAGCTGCTTT 
AAACGTCATG GAGCAAAGGG GATGGACACC CCAGCATTTG AGCTGAAGGA AACCCTGACT 
GAGAAGTATG GAGAGGACTC TGGGCTCATG TATGATCTGA AGGATCAAGG TGGAGAGCTG 
TTGTCCCTCC GCTATGACCT TACTGTTCCC TTTGCTCGTT ATCTGGCCAT GAATAAGGTG 
AAGAAGATGA AACGTTATCA TGTTGGAAAG GTGTGGCGGC GAGAGAGCCC AACCATAGTC 
CAAGGCCGTT ATAGGGAGTT CTGCCAGTGT GATTTTGACA TTGCTGGTCA GTTTGACCCT 
ATGATCCCCG ATGCAGAGTG TTTGAAGATC ATGTGTGAAA TCCTAAGTGG ATTGCAGTTG 
GGAGACTTTC TCATTAAGGT AAATGACCGG CGGATTGTGG ATGGGATGTT TGCTGTCTGT 
GGTGTTCCTG AAAGCAAGTT CCGTGCCATC TGCTCCTCCA TAGATAAACT AGACAAGATG 
GCTTGGAAAG ATGTGAGACA TGAGATGGTG GTGAAGAAAG GCCTGGCTCC TGAGGTGGCT 
GATCGAATTG GGGACTATGT CCAGTGTCAT GGTGGGGTAT CCCTAGTAGA GCAAATGTTT 
CAGGATCCCA GACTATCCCA GAACAAGCAG GCCCTGGAGG GCCTGGGAGA CCTAAAGCTG 
CTATTTGAAT ACCTGACTTT ATTTGGAATT GCTGATAAGA TCTCCTTTGA CCTCAGCCTG 
GCTCGGGGCC TAGACTACTA TACAGGAGTG ATCTATGAAG CAGTGCTGCT GCAGACCCCA 
ACTCAGGCTG GGGAGGAGCC CCTGAATGTG GGCAGTGTGG CTGCTGGTGG GCGCTATGAT 
GGGCTGGTGG GCATGTTTGA CCCCAAGGGC CACAAGGTGC CATGTGTGGG ACTCAGCATT 
GGGGTTGAGC GAATCTTCTA CATTGTGGAG CAGAGGATGA AGACCAAAGG TGAGAAGGTG 
CGGACTACAG AGACTCAAGT GTTTGTGGCC ACACCACAGA AGAACTTTCT CCAAGAACGG 
TTGAAGCTTA TTGCAGAGCT TTGGGATTCT GGAATCAAGG CAGAGATGCT ATACAAGAAC 
AACCCCAAAC TATTAACCCA GCTGCACTAT TGTGAGAGCA CAGGCATTCC ACTGGTGGTC 
ATTATTGGTG AGCAAGAACT GAAAGAAGGG GTCATCAAGA TCCGTTCAGT GGCCAGCAGA 
GAGGAGGTGG CCATTAAACG GGAAAATTTT GTGGCTGAAA TTCAGAAGCG ACTGTCTGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265660.1
CDS	224..1669
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001278731.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278731.1

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TACCAGGGAT 
CTTAGTCCTC AGCATATGGT TGTGAGGGAG AAAATTCTTG ATTTGGTTAT CAGCTGCTTT 
AAACGTCATG GAGCAAAGGG GATGGACACC CCAGCATTTG AGCTGAAGGA AACCCTGACT 
GAGAAGTATG GAGAGGACTC TGGGCTCATG TATGATCTGA AGGATCAAGG TGGAGAGCTG 
TTGTCCCTCC GCTATGACCT TACTGTTCCC TTTGCTCGTT ATCTGGCCAT GAATAAGGTG 
AAGAAGATGA AACGTTATCA TGTTGGAAAG GTGTGGCGGC GAGAGAGCCC AACCATAGTC 
CAAGGCCGTT ATAGGGAGTT CTGCCAGTGT GATTTTGACA TTGCTGGTCA GTTTGACCCT 
ATGATCCCCG ATGCAGAGTG TTTGAAGATC ATGTGTGAAA TCCTAAGTGG ATTGCAGTTG 
GGAGACTTTC TCATTAAGGT AAATGACCGG CGGATTGTGG ATGGGATGTT TGCTGTCTGT 
GGTGTTCCTG AAAGCAAGTT CCGTGCCATC TGCTCCTCCA TAGATAAACT AGACAAGATG 
GCTTGGAAAG ATGTGAGACA TGAGATGGTG GTGAAGAAAG GCCTGGCTCC TGAGGTGGCT 
GATCGAATTG GGGACTATGT CCAGTGTCAT GGTGGGGTAT CCCTAGTAGA GCAAATGTTT 
CAGGATCCCA GACTATCCCA GAACAAGCAG GCCCTGGAGG GCCTGGGAGA CCTAAAGCTG 
CTATTTGAAT ACCTGACTTT ATTTGGAATT GCTGATAAGA TCTCCTTTGA CCTCAGCCTG 
GCTCGGGGCC TAGACTACTA TACAGGAGTG ATCTATGAAG CAGTGCTGCT GCAGACCCCA 
ACTCAGGCTG GGGAGGAGCC CCTGAATGTG GGCAGTGTGG CTGCTGGTGG GCGCTATGAT 
GGGCTGGTGG GCATGTTTGA CCCCAAGGGC CACAAGGTGC CATGTGTGGG ACTCAGCATT 
GGGGTTGAGC GAATCTTCTA CATTGTGGAG CAGAGGATGA AGACCAAAGG TGAGAAGGTG 
CGGACTACAG AGACTCAAGT GTTTGTGGCC ACACCACAGA AGAACTTTCT CCAAGAACGG 
TTGAAGCTTA TTGCAGAGCT TTGGGATTCT GGAATCAAGG CAGAGATGCT ATACAAGAAC 
AACCCCAAAC TATTAACCCA GCTGCACTAT TGTGAGAGCA CAGGCATTCC ACTGGTGGTC 
ATTATTGGTG AGCAAGAACT GAAAGAAGGG GTCATCAAGA TCCGTTCAGT GGCCAGCAGA 
GAGGAGGTGG CCATTAAACG GGAAAATTTT GTGGCTGAAA TTCAGAAGCG ACTGTCTGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001278731.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265660.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09767
Clone ID Related Accession (Same CDS sequence)	NM_001278731.1 , NM_001278731.2
Accession Version	NM_001278731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150729.1, BC014982.1 and AK293390.1. On May 31, 2019 this sequence version replaced NM_001278731.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.256096.1, SRR1660803.257553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TACCAGGGAT 
CTTAGTCCTC AGCATATGGT TGTGAGGGAG AAAATTCTTG ATTTGGTTAT CAGCTGCTTT 
AAACGTCATG GAGCAAAGGG GATGGACACC CCAGCATTTG AGCTGAAGGA AACCCTGACT 
GAGAAGTATG GAGAGGACTC TGGGCTCATG TATGATCTGA AGGATCAAGG TGGAGAGCTG 
TTGTCCCTCC GCTATGACCT TACTGTTCCC TTTGCTCGTT ATCTGGCCAT GAATAAGGTG 
AAGAAGATGA AACGTTATCA TGTTGGAAAG GTGTGGCGGC GAGAGAGCCC AACCATAGTC 
CAAGGCCGTT ATAGGGAGTT CTGCCAGTGT GATTTTGACA TTGCTGGTCA GTTTGACCCT 
ATGATCCCCG ATGCAGAGTG TTTGAAGATC ATGTGTGAAA TCCTAAGTGG ATTGCAGTTG 
GGAGACTTTC TCATTAAGGT AAATGACCGG CGGATTGTGG ATGGGATGTT TGCTGTCTGT 
GGTGTTCCTG AAAGCAAGTT CCGTGCCATC TGCTCCTCCA TAGATAAACT AGACAAGATG 
GCTTGGAAAG ATGTGAGACA TGAGATGGTG GTGAAGAAAG GCCTGGCTCC TGAGGTGGCT 
GATCGAATTG GGGACTATGT CCAGTGTCAT GGTGGGGTAT CCCTAGTAGA GCAAATGTTT 
CAGGATCCCA GACTATCCCA GAACAAGCAG GCCCTGGAGG GCCTGGGAGA CCTAAAGCTG 
CTATTTGAAT ACCTGACTTT ATTTGGAATT GCTGATAAGA TCTCCTTTGA CCTCAGCCTG 
GCTCGGGGCC TAGACTACTA TACAGGAGTG ATCTATGAAG CAGTGCTGCT GCAGACCCCA 
ACTCAGGCTG GGGAGGAGCC CCTGAATGTG GGCAGTGTGG CTGCTGGTGG GCGCTATGAT 
GGGCTGGTGG GCATGTTTGA CCCCAAGGGC CACAAGGTGC CATGTGTGGG ACTCAGCATT 
GGGGTTGAGC GAATCTTCTA CATTGTGGAG CAGAGGATGA AGACCAAAGG TGAGAAGGTG 
CGGACTACAG AGACTCAAGT GTTTGTGGCC ACACCACAGA AGAACTTTCT CCAAGAACGG 
TTGAAGCTTA TTGCAGAGCT TTGGGATTCT GGAATCAAGG CAGAGATGCT ATACAAGAAC 
AACCCCAAAC TATTAACCCA GCTGCACTAT TGTGAGAGCA CAGGCATTCC ACTGGTGGTC 
ATTATTGGTG AGCAAGAACT GAAAGAAGGG GTCATCAAGA TCCGTTCAGT GGCCAGCAGA 
GAGGAGGTGG CCATTAAACG GGAAAATTTT GTGGCTGAAA TTCAGAAGCG ACTGTCTGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265660.1
CDS	195..1640
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001278731.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278731.2

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TACCAGGGAT 
CTTAGTCCTC AGCATATGGT TGTGAGGGAG AAAATTCTTG ATTTGGTTAT CAGCTGCTTT 
AAACGTCATG GAGCAAAGGG GATGGACACC CCAGCATTTG AGCTGAAGGA AACCCTGACT 
GAGAAGTATG GAGAGGACTC TGGGCTCATG TATGATCTGA AGGATCAAGG TGGAGAGCTG 
TTGTCCCTCC GCTATGACCT TACTGTTCCC TTTGCTCGTT ATCTGGCCAT GAATAAGGTG 
AAGAAGATGA AACGTTATCA TGTTGGAAAG GTGTGGCGGC GAGAGAGCCC AACCATAGTC 
CAAGGCCGTT ATAGGGAGTT CTGCCAGTGT GATTTTGACA TTGCTGGTCA GTTTGACCCT 
ATGATCCCCG ATGCAGAGTG TTTGAAGATC ATGTGTGAAA TCCTAAGTGG ATTGCAGTTG 
GGAGACTTTC TCATTAAGGT AAATGACCGG CGGATTGTGG ATGGGATGTT TGCTGTCTGT 
GGTGTTCCTG AAAGCAAGTT CCGTGCCATC TGCTCCTCCA TAGATAAACT AGACAAGATG 
GCTTGGAAAG ATGTGAGACA TGAGATGGTG GTGAAGAAAG GCCTGGCTCC TGAGGTGGCT 
GATCGAATTG GGGACTATGT CCAGTGTCAT GGTGGGGTAT CCCTAGTAGA GCAAATGTTT 
CAGGATCCCA GACTATCCCA GAACAAGCAG GCCCTGGAGG GCCTGGGAGA CCTAAAGCTG 
CTATTTGAAT ACCTGACTTT ATTTGGAATT GCTGATAAGA TCTCCTTTGA CCTCAGCCTG 
GCTCGGGGCC TAGACTACTA TACAGGAGTG ATCTATGAAG CAGTGCTGCT GCAGACCCCA 
ACTCAGGCTG GGGAGGAGCC CCTGAATGTG GGCAGTGTGG CTGCTGGTGG GCGCTATGAT 
GGGCTGGTGG GCATGTTTGA CCCCAAGGGC CACAAGGTGC CATGTGTGGG ACTCAGCATT 
GGGGTTGAGC GAATCTTCTA CATTGTGGAG CAGAGGATGA AGACCAAAGG TGAGAAGGTG 
CGGACTACAG AGACTCAAGT GTTTGTGGCC ACACCACAGA AGAACTTTCT CCAAGAACGG 
TTGAAGCTTA TTGCAGAGCT TTGGGATTCT GGAATCAAGG CAGAGATGCT ATACAAGAAC 
AACCCCAAAC TATTAACCCA GCTGCACTAT TGTGAGAGCA CAGGCATTCC ACTGGTGGTC 
ATTATTGGTG AGCAAGAACT GAAAGAAGGG GTCATCAAGA TCCGTTCAGT GGCCAGCAGA 
GAGGAGGTGG CCATTAAACG GGAAAATTTT GTGGCTGAAA TTCAGAAGCG ACTGTCTGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001363535.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350464.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.

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CloneID	OHu66192
Clone ID Related Accession (Same CDS sequence)	XM_011537619.1 , NM_001363535.1 , NM_001363535.2
Accession Version	NM_001363535.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2. On May 24, 2018 this sequence version replaced XM_011537619.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.131330.1, SRR1803614.277704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350464.1
CDS	224..1762
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGEESLQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001363535.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363535.1

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001363535.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350464.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.

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CloneID	OHu66192
Clone ID Related Accession (Same CDS sequence)	XM_011537619.1 , NM_001363535.1 , NM_001363535.2
Accession Version	NM_001363535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2. On May 31, 2019 this sequence version replaced NM_001363535.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.253901.1, SRR1803614.277704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350464.1
CDS	195..1733
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGEESLQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001363535.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363535.2

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001363536.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350465.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu95786
Clone ID Related Accession (Same CDS sequence)	XM_017009289.1 , NM_001363536.2 , XM_017009288.1 , NM_001363536.1
Accession Version	NM_001363536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2. On May 31, 2019 this sequence version replaced NM_001363536.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (5) is shorter at the N-terminus compared to isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.91329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350465.1
CDS	387..1697
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001363536.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363536.2

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( NM_001363536.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350465.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.

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CloneID	OHu95786
Clone ID Related Accession (Same CDS sequence)	XM_017009289.1 , NM_001363536.2 , XM_017009288.1 , NM_001363536.1
Accession Version	NM_001363536.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2. On May 24, 2018 this sequence version replaced XM_024446008.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (5) is shorter at the N-terminus compared to isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.91329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350465.1
CDS	416..1726
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	NM_001363536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363536.1

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_011537619.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535921.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X1, mRNA.

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CloneID	OHu66192
Clone ID Related Accession (Same CDS sequence)	XM_011537619.1 , NM_001363535.1 , NM_001363535.2
Accession Version	XM_011537619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535921.1
CDS	219..1757
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGEESLQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_011537619.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537619.1

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GGTAAATGAC 
CGGCGGATTG TGGATGGGAT GTTTGCTGTC TGTGGTGTTC CTGAAAGCAA GTTCCGTGCC 
ATCTGCTCCT CCATAGATAA ACTAGACAAG ATGGCTTGGA AAGATGTGAG ACATGAGATG 
GTGGTGAAGA AAGGCCTGGC TCCTGAGGTG GCTGATCGAA TTGGGGACTA TGTCCAGTGT 
CATGGTGGGG TATCCCTAGT AGAGCAAATG TTTCAGGATC CCAGACTATC CCAGAACAAG 
CAGGCCCTGG AGGGCCTGGG AGACCTAAAG CTGCTATTTG AATACCTGAC TTTATTTGGA 
ATTGCTGATA AGATCTCCTT TGACCTCAGC CTGGCTCGGG GCCTAGACTA CTATACAGGA 
GTGATCTATG AAGCAGTGCT GCTGCAGACC CCAACTCAGG CTGGGGAGGA GCCCCTGAAT 
GTGGGCAGTG TGGCTGCTGG TGGGCGCTAT GATGGGCTGG TGGGCATGTT TGACCCCAAG 
GGCCACAAGG TGCCATGTGT GGGACTCAGC ATTGGGGTTG AGCGAATCTT CTACATTGTG 
GAGCAGAGGA TGAAGACCAA AGGTGAGAAG GTGCGGACTA CAGAGACTCA AGTGTTTGTG 
GCCACACCAC AGAAGAACTT TCTCCAAGAA CGGTTGAAGC TTATTGCAGA GCTTTGGGAT 
TCTGGAATCA AGGCAGAGAT GCTATACAAG AACAACCCCA AACTATTAAC CCAGCTGCAC 
TATTGTGAGA GCACAGGCAT TCCACTGGTG GTCATTATTG GTGAGCAAGA ACTGAAAGAA 
GGGGTCATCA AGATCCGTTC AGTGGCCAGC AGAGAGGAGG TGGCCATTAA ACGGGAAAAT 
TTTGTGGCTG AAATTCAGAA GCGACTGTCT GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_011537620.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535922.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.

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CloneID	OHu66193
Clone ID Related Accession (Same CDS sequence)	XM_011537620.1
Accession Version	XM_011537620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GATGGCTTGG 
AAAGATGTGA GACATGAGAT GGTGGTGAAG AAAGGCCTGG CTCCTGAGGT GGCTGATCGA 
ATTGGGGACT ATGTCCAGTG TCATGGTGGG GTATCCCTAG TAGAGCAAAT GTTTCAGGAT 
CCCAGACTAT CCCAGAACAA GCAGGCCCTG GAGGGCCTGG GAGACCTAAA GCTGCTATTT 
GAATACCTGA CTTTATTTGG AATTGCTGAT AAGATCTCCT TTGACCTCAG CCTGGCTCGG 
GGCCTAGACT ACTATACAGG AGTGATCTAT GAAGCAGTGC TGCTGCAGAC CCCAACTCAG 
GCTGGGGAGG AGCCCCTGAA TGTGGGCAGT GTGGCTGCTG GTGGGCGCTA TGATGGGCTG 
GTGGGCATGT TTGACCCCAA GGGCCACAAG GTGCCATGTG TGGGACTCAG CATTGGGGTT 
GAGCGAATCT TCTACATTGT GGAGCAGAGG ATGAAGACCA AAGGTGAGAA GGTGCGGACT 
ACAGAGACTC AAGTGTTTGT GGCCACACCA CAGAAGAACT TTCTCCAAGA ACGGTTGAAG 
CTTATTGCAG AGCTTTGGGA TTCTGGAATC AAGGCAGAGA TGCTATACAA GAACAACCCC 
AAACTATTAA CCCAGCTGCA CTATTGTGAG AGCACAGGCA TTCCACTGGT GGTCATTATT 
GGTGAGCAAG AACTGAAAGA AGGGGTCATC AAGATCCGTT CAGTGGCCAG CAGAGAGGAG 
GTGGCCATTA AACGGGAAAA TTTTGTGGCT GAAATTCAGA AGCGACTGTC TGAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535922.1
CDS	219..1658
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQGEESLQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_011537620.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537620.1

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGG TGAAGAGTCT 
TTGCAGGTTG CAGAGGCAGT GTTAACATCC CAACTGAAAG CACATCAAGA GAAACCAAAT 
TTTATTATCA AGACCCCAAA GGGTACCAGG GATCTTAGTC CTCAGCATAT GGTTGTGAGG 
GAGAAAATTC TTGATTTGGT TATCAGCTGC TTTAAACGTC ATGGAGCAAA GGGGATGGAC 
ACCCCAGCAT TTGAGCTGAA GGAAACCCTG ACTGAGAAGT ATGGAGAGGA CTCTGGGCTC 
ATGTATGATC TGAAGGATCA AGGTGGAGAG CTGTTGTCCC TCCGCTATGA CCTTACTGTT 
CCCTTTGCTC GTTATCTGGC CATGAATAAG GTGAAGAAGA TGAAACGTTA TCATGTTGGA 
AAGGTGTGGC GGCGAGAGAG CCCAACCATA GTCCAAGGCC GTTATAGGGA GTTCTGCCAG 
TGTGATTTTG ACATTGCTGG TCAGTTTGAC CCTATGATCC CCGATGCAGA GTGTTTGAAG 
ATCATGTGTG AAATCCTAAG TGGATTGCAG TTGGGAGACT TTCTCATTAA GATGGCTTGG 
AAAGATGTGA GACATGAGAT GGTGGTGAAG AAAGGCCTGG CTCCTGAGGT GGCTGATCGA 
ATTGGGGACT ATGTCCAGTG TCATGGTGGG GTATCCCTAG TAGAGCAAAT GTTTCAGGAT 
CCCAGACTAT CCCAGAACAA GCAGGCCCTG GAGGGCCTGG GAGACCTAAA GCTGCTATTT 
GAATACCTGA CTTTATTTGG AATTGCTGAT AAGATCTCCT TTGACCTCAG CCTGGCTCGG 
GGCCTAGACT ACTATACAGG AGTGATCTAT GAAGCAGTGC TGCTGCAGAC CCCAACTCAG 
GCTGGGGAGG AGCCCCTGAA TGTGGGCAGT GTGGCTGCTG GTGGGCGCTA TGATGGGCTG 
GTGGGCATGT TTGACCCCAA GGGCCACAAG GTGCCATGTG TGGGACTCAG CATTGGGGTT 
GAGCGAATCT TCTACATTGT GGAGCAGAGG ATGAAGACCA AAGGTGAGAA GGTGCGGACT 
ACAGAGACTC AAGTGTTTGT GGCCACACCA CAGAAGAACT TTCTCCAAGA ACGGTTGAAG 
CTTATTGCAG AGCTTTGGGA TTCTGGAATC AAGGCAGAGA TGCTATACAA GAACAACCCC 
AAACTATTAA CCCAGCTGCA CTATTGTGAG AGCACAGGCA TTCCACTGGT GGTCATTATT 
GGTGAGCAAG AACTGAAAGA AGGGGTCATC AAGATCCGTT CAGTGGCCAG CAGAGAGGAG 
GTGGCCATTA AACGGGAAAA TTTTGTGGCT GAAATTCAGA AGCGACTGTC TGAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009289.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864778.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu95786
Clone ID Related Accession (Same CDS sequence)	XM_017009289.1 , NM_001363536.2 , XM_017009288.1 , NM_001363536.1
Accession Version	XM_017009289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864778.1
CDS	491..1801
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009289.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009289.1

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009288.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864777.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu95786
Clone ID Related Accession (Same CDS sequence)	XM_017009289.1 , NM_001363536.2 , XM_017009288.1 , NM_001363536.1
Accession Version	XM_017009288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864777.1
CDS	498..1808
Translation	MVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009288.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009288.1

ORF Insert Sequence:

ATGGTTGTGA GGGAGAAAAT TCTTGATTTG GTTATCAGCT GCTTTAAACG TCATGGAGCA 
AAGGGGATGG ACACCCCAGC ATTTGAGCTG AAGGAAACCC TGACTGAGAA GTATGGAGAG 
GACTCTGGGC TCATGTATGA TCTGAAGGAT CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT 
GACCTTACTG TTCCCTTTGC TCGTTATCTG GCCATGAATA AGGTGAAGAA GATGAAACGT 
TATCATGTTG GAAAGGTGTG GCGGCGAGAG AGCCCAACCA TAGTCCAAGG CCGTTATAGG 
GAGTTCTGCC AGTGTGATTT TGACATTGCT GGTCAGTTTG ACCCTATGAT CCCCGATGCA 
GAGTGTTTGA AGATCATGTG TGAAATCCTA AGTGGATTGC AGTTGGGAGA CTTTCTCATT 
AAGGTAAATG ACCGGCGGAT TGTGGATGGG ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC 
AAGTTCCGTG CCATCTGCTC CTCCATAGAT AAACTAGACA AGATGGCTTG GAAAGATGTG 
AGACATGAGA TGGTGGTGAA GAAAGGCCTG GCTCCTGAGG TGGCTGATCG AATTGGGGAC 
TATGTCCAGT GTCATGGTGG GGTATCCCTA GTAGAGCAAA TGTTTCAGGA TCCCAGACTA 
TCCCAGAACA AGCAGGCCCT GGAGGGCCTG GGAGACCTAA AGCTGCTATT TGAATACCTG 
ACTTTATTTG GAATTGCTGA TAAGATCTCC TTTGACCTCA GCCTGGCTCG GGGCCTAGAC 
TACTATACAG GAGTGATCTA TGAAGCAGTG CTGCTGCAGA CCCCAACTCA GGCTGGGGAG 
GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT GGTGGGCGCT ATGATGGGCT GGTGGGCATG 
TTTGACCCCA AGGGCCACAA GGTGCCATGT GTGGGACTCA GCATTGGGGT TGAGCGAATC 
TTCTACATTG TGGAGCAGAG GATGAAGACC AAAGGTGAGA AGGTGCGGAC TACAGAGACT 
CAAGTGTTTG TGGCCACACC ACAGAAGAAC TTTCTCCAAG AACGGTTGAA GCTTATTGCA 
GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG ATGCTATACA AGAACAACCC CAAACTATTA 
ACCCAGCTGC ACTATTGTGA GAGCACAGGC ATTCCACTGG TGGTCATTAT TGGTGAGCAA 
GAACTGAAAG AAGGGGTCAT CAAGATCCGT TCAGTGGCCA GCAGAGAGGA GGTGGCCATT 
AAACGGGAAA ATTTTGTGGC TGAAATTCAG AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009291.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864780.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu95787
Clone ID Related Accession (Same CDS sequence)	XM_017009291.1 , XM_017009292.1 , XM_017009290.1 , XM_017009290.2
Accession Version	XM_017009291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864780.1
CDS	701..1561
Translation	MFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009291.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009291.1

ORF Insert Sequence:

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009292.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864781.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu95787
Clone ID Related Accession (Same CDS sequence)	XM_017009291.1 , XM_017009292.1 , XM_017009290.1 , XM_017009290.2
Accession Version	XM_017009292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864781.1
CDS	645..1505
Translation	MFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009292.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009292.1

ORF Insert Sequence:

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009290.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864779.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu95787
Clone ID Related Accession (Same CDS sequence)	XM_017009291.1 , XM_017009292.1 , XM_017009290.1 , XM_017009290.2
Accession Version	XM_017009290.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864779.1
CDS	1008..1868
Translation	MFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009290.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009290.1

ORF Insert Sequence:

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009290.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864779.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu95787
Clone ID Related Accession (Same CDS sequence)	XM_017009291.1 , XM_017009292.1 , XM_017009290.1 , XM_017009290.2
Accession Version	XM_017009290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864779.1
CDS	1032..1892
Translation	MFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009290.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009290.2

ORF Insert Sequence:

ATGTTTGCTG TCTGTGGTGT TCCTGAAAGC AAGTTCCGTG CCATCTGCTC CTCCATAGAT 
AAACTAGACA AGATGGCTTG GAAAGATGTG AGACATGAGA TGGTGGTGAA GAAAGGCCTG 
GCTCCTGAGG TGGCTGATCG AATTGGGGAC TATGTCCAGT GTCATGGTGG GGTATCCCTA 
GTAGAGCAAA TGTTTCAGGA TCCCAGACTA TCCCAGAACA AGCAGGCCCT GGAGGGCCTG 
GGAGACCTAA AGCTGCTATT TGAATACCTG ACTTTATTTG GAATTGCTGA TAAGATCTCC 
TTTGACCTCA GCCTGGCTCG GGGCCTAGAC TACTATACAG GAGTGATCTA TGAAGCAGTG 
CTGCTGCAGA CCCCAACTCA GGCTGGGGAG GAGCCCCTGA ATGTGGGCAG TGTGGCTGCT 
GGTGGGCGCT ATGATGGGCT GGTGGGCATG TTTGACCCCA AGGGCCACAA GGTGCCATGT 
GTGGGACTCA GCATTGGGGT TGAGCGAATC TTCTACATTG TGGAGCAGAG GATGAAGACC 
AAAGGTGAGA AGGTGCGGAC TACAGAGACT CAAGTGTTTG TGGCCACACC ACAGAAGAAC 
TTTCTCCAAG AACGGTTGAA GCTTATTGCA GAGCTTTGGG ATTCTGGAAT CAAGGCAGAG 
ATGCTATACA AGAACAACCC CAAACTATTA ACCCAGCTGC ACTATTGTGA GAGCACAGGC 
ATTCCACTGG TGGTCATTAT TGGTGAGCAA GAACTGAAAG AAGGGGTCAT CAAGATCCGT 
TCAGTGGCCA GCAGAGAGGA GGTGGCCATT AAACGGGAAA ATTTTGTGGC TGAAATTCAG 
AAGCGACTGT CTGAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS2 ( XM_017009287.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864776.1 Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu95785
Clone ID Related Accession (Same CDS sequence)	XM_017009287.1
Accession Version	XM_017009287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable histidine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGATGGCTT GGAAAGATGT GAGACATGAG 
ATGGTGGTGA AGAAAGGCCT GGCTCCTGAG GTGGCTGATC GAATTGGGGA CTATGTCCAG 
TGTCATGGTG GGGTATCCCT AGTAGAGCAA ATGTTTCAGG ATCCCAGACT ATCCCAGAAC 
AAGCAGGCCC TGGAGGGCCT GGGAGACCTA AAGCTGCTAT TTGAATACCT GACTTTATTT 
GGAATTGCTG ATAAGATCTC CTTTGACCTC AGCCTGGCTC GGGGCCTAGA CTACTATACA 
GGAGTGATCT ATGAAGCAGT GCTGCTGCAG ACCCCAACTC AGGCTGGGGA GGAGCCCCTG 
AATGTGGGCA GTGTGGCTGC TGGTGGGCGC TATGATGGGC TGGTGGGCAT GTTTGACCCC 
AAGGGCCACA AGGTGCCATG TGTGGGACTC AGCATTGGGG TTGAGCGAAT CTTCTACATT 
GTGGAGCAGA GGATGAAGAC CAAAGGTGAG AAGGTGCGGA CTACAGAGAC TCAAGTGTTT 
GTGGCCACAC CACAGAAGAA CTTTCTCCAA GAACGGTTGA AGCTTATTGC AGAGCTTTGG 
GATTCTGGAA TCAAGGCAGA GATGCTATAC AAGAACAACC CCAAACTATT AACCCAGCTG 
CACTATTGTG AGAGCACAGG CATTCCACTG GTGGTCATTA TTGGTGAGCA AGAACTGAAA 
GAAGGGGTCA TCAAGATCCG TTCAGTGGCC AGCAGAGAGG AGGTGGCCAT TAAACGGGAA 
AATTTTGTGG CTGAAATTCA GAAGCGACTG TCTGAGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864776.1
CDS	224..1645
Translation	MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES

Target ORF information:

RefSeq Version	XM_017009287.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009287.1

ORF Insert Sequence:

ATGCCCCTGC TCGGACTTCT TCCCAGGAGG GCCTGGGCTT CGCTGCTCAG CCAGCTCCTG 
CGACCGCCCT GCGCTTCGTG CACCGGGGCG GTCCGTTGCC AAAGCCAGGT TGCAGAGGCA 
GTGTTAACAT CCCAACTGAA AGCACATCAA GAGAAACCAA ATTTTATTAT CAAGACCCCA 
AAGGGTACCA GGGATCTTAG TCCTCAGCAT ATGGTTGTGA GGGAGAAAAT TCTTGATTTG 
GTTATCAGCT GCTTTAAACG TCATGGAGCA AAGGGGATGG ACACCCCAGC ATTTGAGCTG 
AAGGAAACCC TGACTGAGAA GTATGGAGAG GACTCTGGGC TCATGTATGA TCTGAAGGAT 
CAAGGTGGAG AGCTGTTGTC CCTCCGCTAT GACCTTACTG TTCCCTTTGC TCGTTATCTG 
GCCATGAATA AGGTGAAGAA GATGAAACGT TATCATGTTG GAAAGGTGTG GCGGCGAGAG 
AGCCCAACCA TAGTCCAAGG CCGTTATAGG GAGTTCTGCC AGTGTGATTT TGACATTGCT 
GGTCAGTTTG ACCCTATGAT CCCCGATGCA GAGTGTTTGA AGATCATGTG TGAAATCCTA 
AGTGGATTGC AGTTGGGAGA CTTTCTCATT AAGATGGCTT GGAAAGATGT GAGACATGAG 
ATGGTGGTGA AGAAAGGCCT GGCTCCTGAG GTGGCTGATC GAATTGGGGA CTATGTCCAG 
TGTCATGGTG GGGTATCCCT AGTAGAGCAA ATGTTTCAGG ATCCCAGACT ATCCCAGAAC 
AAGCAGGCCC TGGAGGGCCT GGGAGACCTA AAGCTGCTAT TTGAATACCT GACTTTATTT 
GGAATTGCTG ATAAGATCTC CTTTGACCTC AGCCTGGCTC GGGGCCTAGA CTACTATACA 
GGAGTGATCT ATGAAGCAGT GCTGCTGCAG ACCCCAACTC AGGCTGGGGA GGAGCCCCTG 
AATGTGGGCA GTGTGGCTGC TGGTGGGCGC TATGATGGGC TGGTGGGCAT GTTTGACCCC 
AAGGGCCACA AGGTGCCATG TGTGGGACTC AGCATTGGGG TTGAGCGAAT CTTCTACATT 
GTGGAGCAGA GGATGAAGAC CAAAGGTGAG AAGGTGCGGA CTACAGAGAC TCAAGTGTTT 
GTGGCCACAC CACAGAAGAA CTTTCTCCAA GAACGGTTGA AGCTTATTGC AGAGCTTTGG 
GATTCTGGAA TCAAGGCAGA GATGCTATAC AAGAACAACC CCAAACTATT AACCCAGCTG 
CACTATTGTG AGAGCACAGG CATTCCACTG GTGGTCATTA TTGGTGAGCA AGAACTGAAA 
GAAGGGGTCA TCAAGATCCG TTCAGTGGCC AGCAGAGAGG AGGTGGCCAT TAAACGGGAA 
AATTTTGTGG CTGAAATTCA GAAGCGACTG TCTGAGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HARS2
Entrez Gene ID	23438
Full Name	histidyl-tRNA synthetase 2, mitochondrial
Synonyms	HARSL,HARSR,HO3,PRLTS2
General protein information	Preferred Names histidyl-tRNA synthetase 2, mitochondrial Names probable histidine--tRNA ligase, mitochondrial HARS-related hisRS histidine tRNA ligase 2, mitochondrial (putative) histidine translase histidine-tRNA ligase homolog histidyl-tRNA synthetase 2, mitochondrial (putative) probable histidyl-tRNA synthetase, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013].
Disorder MIM:	600783
Disorder Html:	?Perrault syndrome 2, 614926 (3)

Pan troglodytes (chimpanzee)	HARS2	XP_517980.3
Bos taurus (cattle)	HARS2	NP_001091602.1
Xenopus tropicalis (tropical clawed frog)	LOC100488813	XP_002944317.2
Homo sapiens (human)	HARS2	NP_036340.1
Rattus norvegicus (Norway rat)	Hars2	NP_001014034.2
Canis lupus familiaris (dog)	HARS2	XP_535214.3
Gallus gallus (chicken)	HARS2	XP_001231932.2
	DTD1	XP_002808484.1
Mus musculus (house mouse)	Hars2	NP_542367.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HARS2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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