CELA3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CELA3B
Entrez Gene ID	23436
Full Name	chymotrypsin like elastase family member 3B
Synonyms	CBPP,ELA3B
General protein information	Preferred Names chymotrypsin like elastase family member 3B Names chymotrypsin-like elastase family member 3B cholesterol-binding pancreatic protease elastase 3B, pancreatic elastase IIIB elastase-3B fecal elastase 1 pancreatic elastase 1 pancreatic endopeptidase E protease E proteinase E
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.12
Summary	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007352.3	NP_031378.1	chymotrypsin-like elastase family member 3B preproprotein
NM_007352.4	NP_031378.1	chymotrypsin-like elastase family member 3B preproprotein

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101734670	XP_004920158.1
Xenopus tropicalis (tropical clawed frog)	LOC100494627	XP_004920157.1
Homo sapiens (human)	CELA3B	NP_031378.1
	CELA3B	XP_001099601.1
Canis lupus familiaris (dog)	CELA3B	XP_535370.1
Mus musculus (house mouse)	Cela3b	NP_080695.1
Rattus norvegicus (Norway rat)	Cela3b	NP_001100162.1
Gallus gallus (chicken)	LOC100857116	XP_003642587.1
Xenopus tropicalis (tropical clawed frog)	cela3a	NP_001011426.1
Pan troglodytes (chimpanzee)	CELA3B	XP_513081.3
Bos taurus (cattle)	CELA3B	NP_001159990.1
Xenopus tropicalis (tropical clawed frog)	LOC101734861	XP_004920970.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.
P?rniczky A, Hegyi E, T?th AZ, Sz?cs ?, Szentesi A, Vincze ?, Izb?ki F, N?meth BC, Hegyi P, Sahin-T?th M
International journal of molecular sciences17(12)(2016 Dec)

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
Szab? A, Pilsak C, Bence M, Witt H, Sahin-T?th M
The Journal of biological chemistry291(34)17706-16(2016 08)

Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus.
Kangrga RN, Ignjatovi? SD, Draga?evi? MM, Jovi?i? S?, Majki?-Singh NT
Laboratory medicine47(2)140-8(2016 May)

Human type I pancreatic elastase treatment of arteriovenous fistulas in patients with chronic kidney disease.
Hye RJ, Peden EK, O'Connor TP, Browne BJ, Dixon BS, Schanzer AS, Jensik SC, Dember LM, Jaff MR, Burke SK
Journal of vascular surgery60(2)454-461.e1(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

variants affecting amino-acid position 241 in CELA3A and CELA3B are not associated with chronic pancreatitis, indicating that changes in complex formation between proelastases and procarboxypeptidases do not alter pancreatitis risk.
Title: Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
Title: Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.

this study demonstrated a strong association of diabetes with low pancreatic elastase levels in feces
Title: Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus.

The results of the study suggest that the levels of apelin, TNF-alpha and elastase-1 are important diagnostic markers of CP in patients with type 2 diabetes mellitus.
Title: [DIAGNOSTIC MARKERS FOR CHRONIC PANCREATITIS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS WITH DIFFERENT PHENOTYPE].

Recombinant type I pancreatic elastase improves unassisted arteriovenous fistula maturation in hemodialysis patients.
Title: Human type I pancreatic elastase treatment of arteriovenous fistulas in patients with chronic kidney disease.

The following CELA3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CELA3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29395	NM_007352.4 Latest version!	Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu29395	NM_007352.3	Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CELA3B ( NM_007352.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_031378.1 Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.

Price & Availability↑

CloneID	OHu29395
Clone ID Related Accession (Same CDS sequence)	NM_007352.3 , NM_007352.4
Accession Version	NM_007352.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-10
Organism	Homo sapiens(human)
Product	chymotrypsin-like elastase family member 3B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI760781.1, AL590556.20, BC005216.1, BM129235.1 and BM128949.1. On Mar 26, 2016 this sequence version replaced NM_007352.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005216.1, M18692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC GCCCTTCCAG CCGCGTTGTC AATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGCGGAAGCT TCTACCACAC CTGTGGCGGT 
AGCCTCATCG CCCCCGACTG GGTTGTGACT GCCGGCCACT GCATCTCGAG CTCCCGGACC 
TACCAGGTGG TGTTGGGCGA GTACGACCGT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGGGGACCT CTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA CGCCGTCCAG 
CTCGCCTCAC TCCCTCCGGC TGGTGACATC CTTCCCAACG AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAACGGGCCA CTCCCAGACA AGCTGCAGGA GGCCCTGCTG 
CCGGTGGTGG ACTATGAACA CTGCTCCAGG TGGAACTGGT GGGGTTCCTC CGTGAAGAAG 
ACCATGGTGT GTGCTGGAGG GGACATCCGC TCCGGCTGCA ATGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCATG GCGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACACCCG CAGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATT 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031378.1
CDS	147..959
Translation	MMLRLLSSLLLVAVASGYGPPSSRPSSRVVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISSSRTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH

Target ORF information:

RefSeq Version	NM_007352.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007352.3

ORF Insert Sequence:

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC GCCCTTCCAG CCGCGTTGTC AATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGCGGAAGCT TCTACCACAC CTGTGGCGGT 
AGCCTCATCG CCCCCGACTG GGTTGTGACT GCCGGCCACT GCATCTCGAG CTCCCGGACC 
TACCAGGTGG TGTTGGGCGA GTACGACCGT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGGGGACCT CTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA CGCCGTCCAG 
CTCGCCTCAC TCCCTCCGGC TGGTGACATC CTTCCCAACG AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAACGGGCCA CTCCCAGACA AGCTGCAGGA GGCCCTGCTG 
CCGGTGGTGG ACTATGAACA CTGCTCCAGG TGGAACTGGT GGGGTTCCTC CGTGAAGAAG 
ACCATGGTGT GTGCTGGAGG GGACATCCGC TCCGGCTGCA ATGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCATG GCGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACACCCG CAGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATT 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CELA3B ( NM_007352.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_031378.1 Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.

Price & Availability↑

CloneID	OHu29395
Clone ID Related Accession (Same CDS sequence)	NM_007352.3 , NM_007352.4
Accession Version	NM_007352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Homo sapiens(human)
Product	chymotrypsin-like elastase family member 3B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005216.1, BM129235.1 and BM128949.1. On May 17, 2019 this sequence version replaced NM_007352.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M18692.1, DRR138519.19030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337107.11/ ENSP00000338369.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC GCCCTTCCAG CCGCGTTGTC AATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGCGGAAGCT TCTACCACAC CTGTGGCGGT 
AGCCTCATCG CCCCCGACTG GGTTGTGACT GCCGGCCACT GCATCTCGAG CTCCCGGACC 
TACCAGGTGG TGTTGGGCGA GTACGACCGT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGGGGACCT CTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA CGCCGTCCAG 
CTCGCCTCAC TCCCTCCGGC TGGTGACATC CTTCCCAACG AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAACGGGCCA CTCCCAGACA AGCTGCAGGA GGCCCTGCTG 
CCGGTGGTGG ACTATGAACA CTGCTCCAGG TGGAACTGGT GGGGTTCCTC CGTGAAGAAG 
ACCATGGTGT GTGCTGGAGG GGACATCCGC TCCGGCTGCA ATGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCATG GCGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACACCCG CAGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATT 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031378.1
CDS	19..831
Translation	MMLRLLSSLLLVAVASGYGPPSSRPSSRVVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISSSRTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH

Target ORF information:

RefSeq Version	NM_007352.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chymotrypsin like elastase 3B (CELA3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007352.4

ORF Insert Sequence:

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC GCCCTTCCAG CCGCGTTGTC AATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGCGGAAGCT TCTACCACAC CTGTGGCGGT 
AGCCTCATCG CCCCCGACTG GGTTGTGACT GCCGGCCACT GCATCTCGAG CTCCCGGACC 
TACCAGGTGG TGTTGGGCGA GTACGACCGT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGGGGACCT CTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA CGCCGTCCAG 
CTCGCCTCAC TCCCTCCGGC TGGTGACATC CTTCCCAACG AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAACGGGCCA CTCCCAGACA AGCTGCAGGA GGCCCTGCTG 
CCGGTGGTGG ACTATGAACA CTGCTCCAGG TGGAACTGGT GGGGTTCCTC CGTGAAGAAG 
ACCATGGTGT GTGCTGGAGG GGACATCCGC TCCGGCTGCA ATGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCATG GCGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACACCCG CAGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATT 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.
International journal of molecular sciences17(12)(2016 Dec)
P?rniczky A,Hegyi E,T?th AZ,Sz?cs ?,Szentesi A,Vincze ?,Izb?ki F,N?meth BC,Hegyi P,Sahin-T?th M

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
The Journal of biological chemistry291(34)17706-16(2016 08)
Szab? A,Pilsak C,Bence M,Witt H,Sahin-T?th M

Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus.
Laboratory medicine47(2)140-8(2016 May)
Kangrga RN,Ignjatovi? SD,Draga?evi? MM,Jovi?i? S?,Majki?-Singh NT

Human type I pancreatic elastase treatment of arteriovenous fistulas in patients with chronic kidney disease.
Journal of vascular surgery60(2)454-461.e1(2014 Aug)
Hye RJ,Peden EK,O'Connor TP,Browne BJ,Dixon BS,Schanzer AS,Jensik SC,Dember LM,Jaff MR,Burke SK

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CELA3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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