TPSD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPSD1
Entrez Gene ID	23430
Full Name	tryptase delta 1
Synonyms	MCP7-LIKE,MCP7L1,MMCP-7L
General protein information	Preferred Names tryptase delta 1 Names tryptase delta delta-tryptase hmMCP-3-like tryptase III mMCP-7-like delta II tryptase mMCP-7-like-1 mMCP-7-like-2 mast cell tryptase tryptase-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. This gene was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	609272

mRNA and Protein(s)

mRNA	Protein	Name
NM_012217.3	NP_036349.1	tryptase delta precursor
NM_012217.2	NP_036349.1	tryptase delta precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TPSD1	NP_036349.1
Pan troglodytes (chimpanzee)	TPSD1	XP_003314949.2

Gene Ontology
Bibliography

Related articles in PubMed

Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.
Trivedi NN, Tamraz B, Chu C, Kwok PY, Caughey GH
The Journal of allergy and clinical immunology124(5)1099-105.e1-4(2009 Nov)

Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation.
Jackson NE, Wang HW, Bryant KJ, McNeil HP, Husain A, Liu K, Tedla N, Thomas PS, King GC, Hettiaratchi A, Cairns J, Hunt JE
The Journal of biological chemistry283(49)34178-87(2008 Dec)

Chimerism, point mutation, and truncation dramatically transformed mast cell delta-tryptases during primate evolution.
Trivedi NN, Raymond WW, Caughey GH
The Journal of allergy and clinical immunology121(5)1262-8(2008 May)

Mast cell alpha and beta tryptases changed rapidly during primate speciation and evolved from gamma-like transmembrane peptidases in ancestral vertebrates.
Trivedi NN, Tong Q, Raman K, Bhagwandin VJ, Caughey GH
Journal of immunology (Baltimore, Md. : 1950)179(9)6072-9(2007 Nov)

Tryptase genetics and anaphylaxis.
Caughey GH
The Journal of allergy and clinical immunology117(6)1411-4(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Delta tryptase transcripts have been detected in a wide variety of tissues including lung, heart, stomach, spleen, skin, and colon, and a recombinant form is proteolytically active.
Title: Delta tryptase is expressed in multiple human tissues, and a recombinant form has proteolytic activity.

The following TPSD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPSD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06112	NM_012217.3 Latest version!	Homo sapiens tryptase delta 1 (TPSD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu06112	NM_012217.2	Homo sapiens tryptase delta 1 (TPSD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPSD1 ( NM_012217.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036349.1 Homo sapiens tryptase delta 1 (TPSD1), mRNA.

Price & Availability↑

CloneID	OHu06112
Clone ID Related Accession (Same CDS sequence)	NM_012217.3 , NM_012217.2
Accession Version	NM_012217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	tryptase delta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC120498.2. On Nov 26, 2018 this sequence version replaced NM_012217.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000211076.5/ ENSP00000211076.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG 
AGCCCGGCCT ACGTGGCCCC TGCCCCAGGC CAGGCCCTGC AGCAAACGGG CATTGTTGGG 
GGGCAGGAGG CCCCCAGGAG CAAGTGGCCC TGGCAGGTGA GCCTGAGAGT CCGCGGCCCA 
TACTGGATGC ACTTCTGCGG GGGCTCCCTC ATCCACCCCC AGTGGGTGCT AACCGCGGCG 
CACTGCGTGG AACCGGACAT CAAGGATCTG GCCGCCCTCA GGGTGCAACT GCGGGAGCAG 
CACCTCTACT ACCAGGACCA GCTGCTGCCG GTCAGCAGGA TCATCGTGCA CCCACAGTTC 
TACATCATCC AGACCGGGGC GGACATCGCC CTGCTGGAGC TGGAGGAGCC CGTGAACATC 
TCCAGCCACA TCCACACGGT CACGCTGCCC CCTGCCTCGG AGACCTTCCC CCCGGGGATG 
CCGTGCTGGG TCACTGGCTG GGGCGACGTG GACAATAATG TGCACCTGCC GCCGCCATAC 
CCGCTGAAGG AGGTGGAAGT CCCCGTAGTG GAAAACCACC TTTGCAACGC GGAATATCAC 
ACCGGCCTCC ATACGGGCCA CAGCTTTCAA ATCGTCCGCG ATGACATGCT GTGTGCGGGG 
AGCGAAAATC ACGACTCCTG CCAGGGTGAC TCTGGAGGGC CCCTGGTCTG CAAGGTGAAT 
GGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036349.1
CDS	213..941
Translation	MLLLAPQMLSLLLLALPVLASPAYVAPAPGQALQQTGIVGGQEAPRSKWPWQVSLRVRGPYWMHFCGGSLIHPQWVLTAAHCVEPDIKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSHIHTVTLPPASETFPPGMPCWVTGWGDVDNNVHLPPPYPLKEVEVPVVENHLCNAEYHTGLHTGHSFQIVRDDMLCAGSENHDSCQGDSGGPLVCKVNGT

Target ORF information:

RefSeq Version	NM_012217.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tryptase delta 1 (TPSD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012217.3

ORF Insert Sequence:

ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG 
AGCCCGGCCT ACGTGGCCCC TGCCCCAGGC CAGGCCCTGC AGCAAACGGG CATTGTTGGG 
GGGCAGGAGG CCCCCAGGAG CAAGTGGCCC TGGCAGGTGA GCCTGAGAGT CCGCGGCCCA 
TACTGGATGC ACTTCTGCGG GGGCTCCCTC ATCCACCCCC AGTGGGTGCT AACCGCGGCG 
CACTGCGTGG AACCGGACAT CAAGGATCTG GCCGCCCTCA GGGTGCAACT GCGGGAGCAG 
CACCTCTACT ACCAGGACCA GCTGCTGCCG GTCAGCAGGA TCATCGTGCA CCCACAGTTC 
TACATCATCC AGACCGGGGC GGACATCGCC CTGCTGGAGC TGGAGGAGCC CGTGAACATC 
TCCAGCCACA TCCACACGGT CACGCTGCCC CCTGCCTCGG AGACCTTCCC CCCGGGGATG 
CCGTGCTGGG TCACTGGCTG GGGCGACGTG GACAATAATG TGCACCTGCC GCCGCCATAC 
CCGCTGAAGG AGGTGGAAGT CCCCGTAGTG GAAAACCACC TTTGCAACGC GGAATATCAC 
ACCGGCCTCC ATACGGGCCA CAGCTTTCAA ATCGTCCGCG ATGACATGCT GTGTGCGGGG 
AGCGAAAATC ACGACTCCTG CCAGGGTGAC TCTGGAGGGC CCCTGGTCTG CAAGGTGAAT 
GGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPSD1 ( NM_012217.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036349.1 Homo sapiens tryptase delta 1 (TPSD1), mRNA.

Price & Availability↑

CloneID	OHu06112
Clone ID Related Accession (Same CDS sequence)	NM_012217.3 , NM_012217.2
Accession Version	NM_012217.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	tryptase delta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF098327.1. On Jul 8, 2004 this sequence version replaced NM_012217.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG 
AGCCCGGCCT ACGTGGCCCC TGCCCCAGGC CAGGCCCTGC AGCAAACGGG CATTGTTGGG 
GGGCAGGAGG CCCCCAGGAG CAAGTGGCCC TGGCAGGTGA GCCTGAGAGT CCGCGGCCCA 
TACTGGATGC ACTTCTGCGG GGGCTCCCTC ATCCACCCCC AGTGGGTGCT AACCGCGGCG 
CACTGCGTGG AACCGGACAT CAAGGATCTG GCCGCCCTCA GGGTGCAACT GCGGGAGCAG 
CACCTCTACT ACCAGGACCA GCTGCTGCCG GTCAGCAGGA TCATCGTGCA CCCACAGTTC 
TACATCATCC AGACCGGGGC GGACATCGCC CTGCTGGAGC TGGAGGAGCC CGTGAACATC 
TCCAGCCACA TCCACACGGT CACGCTGCCC CCTGCCTCGG AGACCTTCCC CCCGGGGATG 
CCGTGCTGGG TCACTGGCTG GGGCGACGTG GACAATAATG TGCACCTGCC GCCGCCATAC 
CCGCTGAAGG AGGTGGAAGT CCCCGTAGTG GAAAACCACC TTTGCAACGC GGAATATCAC 
ACCGGCCTCC ATACGGGCCA CAGCTTTCAA ATCGTCCGCG ATGACATGCT GTGTGCGGGG 
AGCGAAAATC ACGACTCCTG CCAGGGTGAC TCTGGAGGGC CCCTGGTCTG CAAGGTGAAT 
GGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036349.1
CDS	10..738
Translation	MLLLAPQMLSLLLLALPVLASPAYVAPAPGQALQQTGIVGGQEAPRSKWPWQVSLRVRGPYWMHFCGGSLIHPQWVLTAAHCVEPDIKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSHIHTVTLPPASETFPPGMPCWVTGWGDVDNNVHLPPPYPLKEVEVPVVENHLCNAEYHTGLHTGHSFQIVRDDMLCAGSENHDSCQGDSGGPLVCKVNGT

Target ORF information:

RefSeq Version	NM_012217.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tryptase delta 1 (TPSD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012217.2

ORF Insert Sequence:

ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG 
AGCCCGGCCT ACGTGGCCCC TGCCCCAGGC CAGGCCCTGC AGCAAACGGG CATTGTTGGG 
GGGCAGGAGG CCCCCAGGAG CAAGTGGCCC TGGCAGGTGA GCCTGAGAGT CCGCGGCCCA 
TACTGGATGC ACTTCTGCGG GGGCTCCCTC ATCCACCCCC AGTGGGTGCT AACCGCGGCG 
CACTGCGTGG AACCGGACAT CAAGGATCTG GCCGCCCTCA GGGTGCAACT GCGGGAGCAG 
CACCTCTACT ACCAGGACCA GCTGCTGCCG GTCAGCAGGA TCATCGTGCA CCCACAGTTC 
TACATCATCC AGACCGGGGC GGACATCGCC CTGCTGGAGC TGGAGGAGCC CGTGAACATC 
TCCAGCCACA TCCACACGGT CACGCTGCCC CCTGCCTCGG AGACCTTCCC CCCGGGGATG 
CCGTGCTGGG TCACTGGCTG GGGCGACGTG GACAATAATG TGCACCTGCC GCCGCCATAC 
CCGCTGAAGG AGGTGGAAGT CCCCGTAGTG GAAAACCACC TTTGCAACGC GGAATATCAC 
ACCGGCCTCC ATACGGGCCA CAGCTTTCAA ATCGTCCGCG ATGACATGCT GTGTGCGGGG 
AGCGAAAATC ACGACTCCTG CCAGGGTGAC TCTGGAGGGC CCCTGGTCTG CAAGGTGAAT 
GGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.
The Journal of allergy and clinical immunology124(5)1099-105.e1-4(2009 Nov)
Trivedi NN,Tamraz B,Chu C,Kwok PY,Caughey GH

Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation.
The Journal of biological chemistry283(49)34178-87(2008 Dec)
Jackson NE,Wang HW,Bryant KJ,McNeil HP,Husain A,Liu K,Tedla N,Thomas PS,King GC,Hettiaratchi A,Cairns J,Hunt JE

Chimerism, point mutation, and truncation dramatically transformed mast cell delta-tryptases during primate evolution.
The Journal of allergy and clinical immunology121(5)1262-8(2008 May)
Trivedi NN,Raymond WW,Caughey GH

Mast cell alpha and beta tryptases changed rapidly during primate speciation and evolved from gamma-like transmembrane peptidases in ancestral vertebrates.
Journal of immunology (Baltimore, Md. : 1950)179(9)6072-9(2007 Nov)
Trivedi NN,Tong Q,Raman K,Bhagwandin VJ,Caughey GH

Tryptase genetics and anaphylaxis.
The Journal of allergy and clinical immunology117(6)1411-4(2006 Jun)
Caughey GH

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

TPSD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報