TMED3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMED3
Entrez Gene ID	23423
Full Name	transmembrane p24 trafficking protein 3
Synonyms	C15orf22,P24B,p24g4,p26
General protein information	Preferred Names transmembrane p24 trafficking protein 3 Names transmembrane emp24 domain-containing protein 3 integral type I protein membrane protein p24B p24 family protein gamma-4 p24gamma4 testis tissue sperm-binding protein Li 48e transmembrane emp24 protein transport domain containing 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q25.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017022041.1	XP_016877530.1	transmembrane emp24 domain-containing protein 3 isoform X2
NM_007364.3	NP_031390.1	transmembrane emp24 domain-containing protein 3 isoform a precursor
NM_001330376.1	NP_001317305.1	transmembrane emp24 domain-containing protein 3 isoform c precursor
NM_001301203.2	NP_001288132.1	transmembrane emp24 domain-containing protein 3 isoform b precursor
NM_001301203.1	NP_001288132.1	transmembrane emp24 domain-containing protein 3 isoform b precursor
NM_007364.4	NP_031390.1	transmembrane emp24 domain-containing protein 3 isoform a precursor
NM_001363735.1	NP_001350664.1	transmembrane emp24 domain-containing protein 3 isoform d precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmed3	NP_001004249.1
Bos taurus (cattle)	LOC539132	NP_001075078.1
	TMED3	XP_001109063.1
Canis lupus familiaris (dog)	TMED3	XP_005618488.1
Homo sapiens (human)	TMED3	NP_031390.1
Pan troglodytes (chimpanzee)	TMED3	XP_001153825.1
Mus musculus (house mouse)	Tmed3	NP_079636.2
Gallus gallus (chicken)	TMED3	XP_413948.3
Danio rerio (zebrafish)	tmed3	NP_001122140.1

Gene Ontology
Bibliography

Related articles in PubMed

TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling.
Zheng H, Yang Y, Han J, Jiang WH, Chen C, Wang MC, Gao R, Li S, Tian T, Wang J, Ma LJ, Ren H, Zhou WP
Scientific reports637070(2016 Nov)

A novel genome-wide in vivo screen for metastatic suppressors in human colon cancer identifies the positive WNT-TCF pathway modulators TMED3 and SOX12.
Duquet A, Melotti A, Mishra S, Malerba M, Seth C, Conod A, Ruiz i Altaba A
EMBO molecular medicine6(7)882-901(2014 Jul)

High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.
Vainio P, Mpindi JP, Kohonen P, Fey V, Mirtti T, Alanen KA, Per?l? M, Kallioniemi O, Iljin K
PloS one7(6)e39801(2012)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway.
Jenne N, Frey K, Brugger B, Wieland FT
The Journal of biological chemistry277(48)46504-11(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This genome-wide screen has identified two novel metastatic suppressors: TMED3 and SOX12, the knockdown of which increases metastatic growth after direct seeding.
Title: A novel genome-wide in vivo screen for metastatic suppressors in human colon cancer identifies the positive WNT-TCF pathway modulators TMED3 and SOX12.

AIM1, ERGIC1, and TPX2 were shown to be highly expressed especially in prostate cancer tissues, and high mRNA expression of ERGIC1 and TMED3 associated with AR and ERG oncogene expression
Title: High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.

The following TMED3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMED3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu48195	NM_001301203.2 Latest version!	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu66174	NM_007364.4 Latest version!	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu102140	NM_001330376.1 Latest version!	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu85331	NM_001363735.1 Latest version!	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu85331	XM_017022041.1 Latest version!	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu48195	NM_001301203.1	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu66174	NM_007364.3	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMED3 ( NM_001301203.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288132.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu48195
Clone ID Related Accession (Same CDS sequence)	NM_001301203.2 , NM_001301203.1
Accession Version	NM_001301203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011797.8, KF459564.1 and AC027811.9. On May 31, 2019 this sequence version replaced NM_001301203.1. Transcript Variant: This variant (2) contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK304148.1, DC416437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGT 
CCCAGTGCTT CACCAACACC AACGCCACCA CTAAGGGTTC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288132.1
CDS	139..606
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQGPSASPTPTPPLRVPV

Target ORF information:

RefSeq Version	NM_001301203.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301203.2

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGT 
CCCAGTGCTT CACCAACACC AACGCCACCA CTAAGGGTTC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( NM_001301203.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288132.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu48195
Clone ID Related Accession (Same CDS sequence)	NM_001301203.2 , NM_001301203.1
Accession Version	NM_001301203.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP260160.1, AK304148.1, AC027811.9 and AI243096.1. On Jul 24, 2014 this sequence version replaced XM_005254263.2. Transcript Variant: This variant (2) contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK304148.1, DC416437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGT 
CCCAGTGCTT CACCAACACC AACGCCACCA CTAAGGGTTC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288132.1
CDS	189..656
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQGPSASPTPTPPLRVPV

Target ORF information:

RefSeq Version	NM_001301203.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301203.1

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGT 
CCCAGTGCTT CACCAACACC AACGCCACCA CTAAGGGTTC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( NM_007364.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_031390.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu66174
Clone ID Related Accession (Same CDS sequence)	NM_007364.3 , NM_007364.4
Accession Version	NM_007364.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP260160.1, BC010853.1, BM699123.1 and BC022232.1. On Jul 25, 2014 this sequence version replaced NM_007364.2. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC022232.1, SRR1660807.262157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGATG 
GAGTCCGCCT GCGTGACCAT CCATGAGGCT CTGAAAACGG TGATTGACTC CCAGACGCAT 
TACCGGCTGC GGGAGGCCCA GGACCGGGCC CGAGCAGAAG ACCTTAATAG CCGAGTCTCT 
TACTGGTCTG TTGGCGAGAC GATTGCCCTG TTCGTGGTCA GCTTCAGTCA GGTGCTACTG 
TTGAAAAGCT TCTTCACAGA AAAACGACCC ATCAGCAGGG CAGTCCACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031390.1
CDS	189..842
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQMESACVTIHEALKTVIDSQTHYRLREAQDRARAEDLNSRVSYWSVGETIALFVVSFSQVLLLKSFFTEKRPISRAVHS

Target ORF information:

RefSeq Version	NM_007364.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007364.3

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGATG 
GAGTCCGCCT GCGTGACCAT CCATGAGGCT CTGAAAACGG TGATTGACTC CCAGACGCAT 
TACCGGCTGC GGGAGGCCCA GGACCGGGCC CGAGCAGAAG ACCTTAATAG CCGAGTCTCT 
TACTGGTCTG TTGGCGAGAC GATTGCCCTG TTCGTGGTCA GCTTCAGTCA GGTGCTACTG 
TTGAAAAGCT TCTTCACAGA AAAACGACCC ATCAGCAGGG CAGTCCACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( NM_007364.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_031390.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu66174
Clone ID Related Accession (Same CDS sequence)	NM_007364.3 , NM_007364.4
Accession Version	NM_007364.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011797.8, KF459564.1 and AC027811.9. On Nov 23, 2018 this sequence version replaced NM_007364.3. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.519148.1, SRR3476690.664899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299705.10/ ENSP00000299705.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGATG 
GAGTCCGCCT GCGTGACCAT CCATGAGGCT CTGAAAACGG TGATTGACTC CCAGACGCAT 
TACCGGCTGC GGGAGGCCCA GGACCGGGCC CGAGCAGAAG ACCTTAATAG CCGAGTCTCT 
TACTGGTCTG TTGGCGAGAC GATTGCCCTG TTCGTGGTCA GCTTCAGTCA GGTGCTACTG 
TTGAAAAGCT TCTTCACAGA AAAACGACCC ATCAGCAGGG CAGTCCACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031390.1
CDS	139..792
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQMESACVTIHEALKTVIDSQTHYRLREAQDRARAEDLNSRVSYWSVGETIALFVVSFSQVLLLKSFFTEKRPISRAVHS

Target ORF information:

RefSeq Version	NM_007364.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007364.4

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGATG 
GAGTCCGCCT GCGTGACCAT CCATGAGGCT CTGAAAACGG TGATTGACTC CCAGACGCAT 
TACCGGCTGC GGGAGGCCCA GGACCGGGCC CGAGCAGAAG ACCTTAATAG CCGAGTCTCT 
TACTGGTCTG TTGGCGAGAC GATTGCCCTG TTCGTGGTCA GCTTCAGTCA GGTGCTACTG 
TTGAAAAGCT TCTTCACAGA AAAACGACCC ATCAGCAGGG CAGTCCACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( NM_001330376.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317305.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu102140
Clone ID Related Accession (Same CDS sequence)	NM_001330376.1
Accession Version	NM_001330376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform c precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP260160.1, AK297710.1, AC027811.9 and AL109713.1. On Aug 29, 2016 this sequence version replaced XM_017022040.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGAGA 
TTCAGAGGAG CTTATTGGAA GGAGGTGGAC AAGATGGTCG ACTATATGCA GCCAGGCGGA 
ACACCTGCCA CCGAGGGACT GGGACGACTG GCACCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317305.1
CDS	189..710
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQRFRGAYWKEVDKMVDYMQPGGTPATEGLGRLAPS

Target ORF information:

RefSeq Version	NM_001330376.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330376.1

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGAGA 
TTCAGAGGAG CTTATTGGAA GGAGGTGGAC AAGATGGTCG ACTATATGCA GCCAGGCGGA 
ACACCTGCCA CCGAGGGACT GGGACGACTG GCACCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( NM_001363735.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350664.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 5, mRNA.

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CloneID	OHu85331
Clone ID Related Accession (Same CDS sequence)	XM_017022041.1 , NM_001363735.1
Accession Version	NM_001363735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform d precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011797.8 and KF459564.1. On May 29, 2018 this sequence version replaced XM_017022041.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.522766.1, DB031895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGC 
TTCAGGGTCT TCTGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350664.1
CDS	189..629
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQGFRVFCL

Target ORF information:

RefSeq Version	NM_001363735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363735.1

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGC 
TTCAGGGTCT TCTGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED3 ( XM_017022041.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877530.1 Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu85331
Clone ID Related Accession (Same CDS sequence)	XM_017022041.1 , NM_001363735.1
Accession Version	XM_017022041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGC 
TTCAGGGTCT TCTGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877530.1
CDS	138..578
Translation	MGSTVPRSASVLLLLLLLRRAEQPCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQGFRVFCL

Target ORF information:

RefSeq Version	XM_017022041.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 3 (TMED3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022041.1

ORF Insert Sequence:

ATGGGCAGCA CTGTCCCGCG CTCCGCCTCC GTGCTGCTTC TGCTGCTGCT CCTGCGCCGG 
GCCGAGCAGC CCTGCGGGGC CGAGCTCACC TTCGAGCTGC CGGACAACGC CAAGCAGTGC 
TTCCACGAGG AGGTGGAGCA GGGCGTGAAG TTCTCCCTGG ATTACCAGGT CATCACTGGA 
GGCCACTACG ATGTTGACTG CTATGTAGAG GACCCCCAGG GGAACACCAT CTACAGAGAA 
ACGAAGAAGC AGTACGACAG CTTCACGTAC CGGGCTGAAG TCAAGGGCGT TTATCAGTTT 
TGCTTCAGTA ATGAGTTTTC CACCTTCTCT CACAAGACCG TCTACTTTGA CTTTCAAGTG 
GGCGATGAGC CTCCCATTCT CCCAGACATG GGGAACAGGG TCACAGCTCT CACCCAGGGC 
TTCAGGGTCT TCTGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling.
Scientific reports637070(2016 Nov)
Zheng H,Yang Y,Han J,Jiang WH,Chen C,Wang MC,Gao R,Li S,Tian T,Wang J,Ma LJ,Ren H,Zhou WP

A novel genome-wide in vivo screen for metastatic suppressors in human colon cancer identifies the positive WNT-TCF pathway modulators TMED3 and SOX12.
EMBO molecular medicine6(7)882-901(2014 Jul)
Duquet A,Melotti A,Mishra S,Malerba M,Seth C,Conod A,Ruiz i Altaba A

High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.
PloS one7(6)e39801(2012)
Vainio P,Mpindi JP,Kohonen P,Fey V,Mirtti T,Alanen KA,Per?l? M,Kallioniemi O,Iljin K

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway.
The Journal of biological chemistry277(48)46504-11(2002 Nov)
Jenne N,Frey K,Brugger B,Wieland FT

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TMED3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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