SMCHD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCHD1
Entrez Gene ID	23347
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
Synonyms	BAMS,FSHD2
General protein information	Preferred Names structural maintenance of chromosomes flexible hinge domain containing 1 Names structural maintenance of chromosomes flexible hinge domain-containing protein 1 SMC hinge domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.32
Summary	This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family. [provided by RefSeq, Dec 2011].
Disorder MIM:	614982
Disorder Html:	Fascioscapulohumeral muscular dystrophy 2, digenic, 158901 (3); Bosma arhinia microphthalmia syndrome, 603457 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015295.2	NP_056110.2	structural maintenance of chromosomes flexible hinge domain-containing protein 1
XM_011525644.2	XP_011523946.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
NM_015295.3	NP_056110.2	structural maintenance of chromosomes flexible hinge domain-containing protein 1
XM_011525643.2	XP_011523945.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
XM_011525642.1	XP_011523944.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
XM_017025684.1	XP_016881173.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCHD1	XP_005623086.1
Bos taurus (cattle)	SMCHD1	NP_001193329.1
Gallus gallus (chicken)	SMCHD1	XP_419144.4
Danio rerio (zebrafish)	LOC570784	XP_699397.5
Pan troglodytes (chimpanzee)	SMCHD1	XP_512045.2
	SMCHD1	XP_001086253.2
Xenopus tropicalis (tropical clawed frog)	smchd1	XP_002938954.1
Homo sapiens (human)	SMCHD1	NP_056110.2
Mus musculus (house mouse)	Smchd1	NP_083163.3
Rattus norvegicus (Norway rat)	Smchd1	XP_244261.6

Gene Ontology

The following SMCHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18596	NM_015295.3 Latest version!	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu66024	XM_011525644.2 Latest version!	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66025	XM_017025684.1 Latest version!	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66022	XM_011525642.1 Latest version!	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu66023	XM_011525643.2 Latest version!	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu18596	NM_015295.2	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCHD1 ( NM_015295.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056110.2 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

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CloneID	OHu18596
Clone ID Related Accession (Same CDS sequence)	NM_015295.2 , NM_015295.3
Accession Version	NM_015295.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006008.1, AI904018.1, AP001011.6, DN913642.1, BU153211.1, BP872870.1, DB071574.1, BU509173.1, AW503637.1, DA283721.1, DA675822.1 and AB014550.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 26, 2007 this sequence version replaced XM_113962.7, XM_938891.2, NM_015295.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGGATTATA 
ACCAAAACAG ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056110.2
CDS	190..6207
Translation	MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPLQVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	NM_015295.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015295.2

ORF Insert Sequence:

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGGATTATA 
ACCAAAACAG ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( NM_015295.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056110.2 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

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CloneID	OHu18596
Clone ID Related Accession (Same CDS sequence)	NM_015295.2 , NM_015295.3
Accession Version	NM_015295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP005061.3, BC006008.1, AI904018.1, AP001011.6, DN913642.1, BU153211.1, BP872870.1, DB071574.1, BU509173.1, AW503637.1, DA283721.1, DA675822.1 and AB014550.1. On Nov 30, 2019 this sequence version replaced NM_015295.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGGATTATA 
ACCAAAACAG ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056110.2
CDS	351..6368
Translation	MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPLQVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDV

Target ORF information:

RefSeq Version	NM_015295.3
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015295.3

ORF Insert Sequence:

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGGATTATA 
ACCAAAACAG ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_011525644.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523946.1 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.

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CloneID	OHu66024
Clone ID Related Accession (Same CDS sequence)	XM_011525644.2
Accession Version	XM_011525644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAT ATTATGCCAG TGAAGGACAA AATCCTTTGC CATTTGCTCT TGCGGAATTA 
ATTGACAATT CATTGTCTGC TACTTCTCGT AACATTGGGG TTAGAAGAAT ACAGATCAAA 
TTGCTTTTTG ATGAAACACA AGGAAAACCT GCTGTTGCAG TGATAGATAA TGGAAGAGGA 
ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT CACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGTCCAG TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAAG CTGTCTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAAGATG TTCACGAGCT TGTGCTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCAA TATATAGTGG ATATATTAGA 
AACAGAAAGC CCTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCATAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCACCTTT GGACACGACA GTTAGCGCAT 
ATTTATCACT ACTATATTCA TGGCCCAAAA GGAAATGAAA TACGAACATC AAAAGAAGTT 
GAACCTTTCA ACAATATTGA TATTGAAATT TCTATGTTTG AAAAAGGGAA GGTACCTAAG 
ATTGTCAACC TAAGGGAAAT ACAAGACGAC ATGCAGACGT TGTATGTAAA CACAGCAGCT 
GATAGTTTTG AATTCAAAGC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGT 
TATCATCCAT TCTTATATGA TAGAGAAACT TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA AGATGATTGT TTCATACTTG AGAAAGCAGC TAGAGGGAAA 
AGGCCTATTT TTGAATGTTT TTGGAATGGA CGATTAATAC CATATACATC AGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CAATTGAATG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ATTGACGTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACCCTTT TTACAAGGAT TTTAAATGGA 
CAGGAACAGC GAATGAAAAT TGACAGAGAA TTTGCTTTGT GGCTGAAGGA CTGTCATGAG 
AAGTATGATA AACAAATAAA ATTTACACTT TTTAAGGGAG TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAAGGTCC CTGGGCAACA TATGCAGCAA TAGAATGGGA TGGAAAGATA 
TACAAAGCAG GACAGCTGGT CAAGACAATC AAGACACTTC CCCTCTTTTA TGGAAGCATA 
GTAAGATTTT TTCTTTATGG CGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA GGCACTATAT GATGAAGTAA GAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTT AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGATG AATTATTGCC TAATGAGGTT 
AGGCCTGCTG GAACCCCTAT TGGTGCGTTA AGAATTGAAA TACTGAATAA AAAAGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGCCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATTT TACATTCTTC AAGTGGAAAT AAAGAGATTA TTTCGCATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAGAAGTT GGGGAATTAT 
ACCTTGAAAT TACAAGTTGT GTTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA GTTTTCTGTT AAAGAGGGTA AGCCAGAGAA ATTTTCATTT 
GGTCTTCTGG ATCTTCCTTT TCGTGTTGGA GTTCCATTTA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACCAG TCAACTAGTA ACTGATATTC AGCCAGTTCT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AAAGGACCAA ATTGTGTAAT TCGAGGTGTT 
ACAGCCAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT ATAATCTGAA GGTTACTCTG 
CCTGGCTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTACCTGG TCACCCTCGT 
CGACTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTGG AAGTTTTAGA TGAATCAGAC AACATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTATG TTGTAGATTG CAGTAGTTCT 
GGAACCAGTA TTTTAACAGG ATCTGCAATT CAAGTTCAGA ATATTAAAAA AGACCAGACG 
CTTAAAGCAA GAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA CTACAAATAT TCAGTGTAGA AGGACAAAAG 
GCAATTCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CGGGTGATAT TATGCATAAT 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCA ATATAACATC AGCTTTAGCA 
GAAAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AACACTTGCT ACAGGGTCTG 
CTTCCTGATG TGCAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCG TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATTTAA AATGTGAAAT GAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAGTAG TTATAATAAT TACAGATCAG TACGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTTTAT CTTCTTTGTC AATTGCTGGG GTTGGACTTG ATAGCTCAAA TTTGAAAACA 
ACCTTTCAGG AAAACACACA GAGTATAAGT GTAAGAGGCA TCAAATTTAT TCCAGGTCCT 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAA 
CTAATTTCTG GACCTCCTGC TAAACTTCTC CTTATAGACT GGCCAGAACT AAAGGAGTCC 
ATTCCAGTGA TTAATGGAAG AGATTTACAG AACCCTATTA TTGTTCAACT TTGTGATCAG 
TGGGATAATC CAGCACCGGT ACAACATGTT AAAATAAGTC TTACAAAAGC TAGCAATTTA 
AAGCTCATGC CTTCAAACCA ACAGCATAAA ACAGATGAGA AAGGCAGGGC TAATTTGGGA 
GTATTCAGTG TTTTTGCCCC TAGGGGAGAG CATACTCTTC AGGTTAAAGC CATCTATAAC 
AAAAGTATCA TAGAAGGACC TATAATTAAG TTAATGATTC TTCCAGACCC AGAAAAACCC 
GTTCGTCTCA ATGTTAAATA TGACAAAGAT GCATCCTTCT TAGCAGGGGG TCTTTTCACT 
GATTTTATGA TTAGTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAACAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAGCTGTCT ACCAGTGGGA ACCGACCCCC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAAGAT AATGACAAAG AAGATGGCTG CTTCTATTTC 
AGGGATAAAG TAATTCCTAA TAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAACAA ATATTCTCAA CAGTGAACAG GTTATAGTTG AAGTCCTGCC TAATCAACCT 
GTGAAGTTAG TACCTAAAAT TAAACCACCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAG AGATCTACAT CTTAGTATCA CGGATGACTA CGACAACCAT 
ACTGGAATTG ATTTGGTTGG CACTATAATA GCCACCATTA AAGGCTCTAA TGAGGAAGAT 
ACTGATACCC CACTTTTTAT TGGGAAAGTT AGAACACTTG AATTCCCCTT CGTGAATGGT 
TCGGCTGAAA TCATGAGTCT GGTGCTGGCA GAAAGTAGTC CTGGAAGGGA TAGTACTGAA 
TATTTTATTG TATTTGAGCC CCGGCTACCA CTTTTATCAA GAACCTTAGA ACCATATATC 
CTACCGTTCA TGTTTTACAA TGATGTTAAG AAGCAGCAAC AAATGGCAGC ACTTACAAAA 
GAAAAGGACC AATTATCTCA GTCTATTGTT ATGTATAAAA GTTTATTTGA AGCCAGCCAA 
CAGCTTCTTA ATGAAATGAA ATGTCAAGTT GAAGAAGCAA GATTAAAAGA GGCCCAATTG 
CGAAATGAAC TAAAAATACA TAATATTGAC ATTCCTACAA CACAACAGGT GCCACACATT 
GAAGCACTTC TGAAAAGAAA GCTATCAGAA CAAGAAGAAC TGAAGAAAAA ACCTAGAAGA 
TCGTGTACTC TTCCAAACTA TACTAAAGGC AGTGGAGATG TTTTGGGAAA GATTGCACAT 
CTAGCACAAA TTGAAGATGA TAGAGCTGCG ATGGTTATTT CTTGGCATCT GGCAAGTGAC 
ATGGACTGTG TAGTCACCCT AACCACTGAC GCTGCACGTC GTATCTATGA TGAAACCCAA 
GGTCGTCAGC AGGTGTTGCC CCTTGATTCT ATTTACAAGA AGACTCTTCC AGATTGGAAA 
AGATCTCTAC CTCATTTCCG AAATGGAAAA TTGTATTTTA AACCCATTGG AGATCCAGTC 
TTTGCTCGAG ACTTGTTAAC ATTTCCAGAT AATGTAGAAC ATTGTGAAAC AGTATTTGGT 
ATGCTGTTAG GAGACACCAT TATTTTGGAT AATCTGGATG CGGCCAATCA TTATAGAAAA 
GAGGTTGTTA AAATTACACA CTGTCCTACA CTGCTGACCA GAGATGGAGA TCGAATTCGA 
AGTAATGGAA AGTTTGGGGG CCTTCAGAAT AAAGCTCCTC CAATGGATAA ACTTCGGGGA 
ATGGTATTTG GAGCTCCAGT TCCAAAACAG TGTCTGATCT TAGGGGAACA AATAGATCTT 
CTTCAGCAGT ATCGTTCTGC TGTGTGCAAA CTAGACAGTG TGAATAAGGA TCTTAACAGT 
CAATTAGAGT ACCTTCGCAC TCCGGATATG AGGAAGAAAA AGCAAGAACT TGATGAACAT 
GAGAAAAATC TCAAACTAAT AGAGGAAAAA CTAGGTATGA CTCCCATACG TAAGTGTAAT 
GACTCATTGC GTCATTCACC AAAGGTTGAG ACGACAGATT GTCCAGTTCC TCCTAAAAGA 
ATGAGACGAG AAGCTACAAG ACAAAATAGT CTTCACAATT CAGAAGACAG CAAATCTCAA 
TTAGGACTAG CTACGTTGCA AGTACTCTGG AGCCACACAC ATGGCTGGTG GCTACCGTTT 
TGGACTGCAC AGGATTATAA CCAAAACAGA TGTATGAGAG GTGACAGAGA GAAGAGGCCA 
TTGGTCTCAG TAAGAATGCC CTGCTTTCTG CATCTCTGTT TCAGAAGACC AAGAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523946.1
CDS	482..6301
Translation	MYEYYASEGQNPLPFALAELIDNSLSATSRNIGVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNSLHNSEDSKSQLGLATLQVLWSHTHGWWLPFWTAQDYNQNRCMRGDREKRPLVSVRMPCFLHLCFRRPRG

Target ORF information:

RefSeq Version	XM_011525644.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525644.2

ORF Insert Sequence:

ATGTATGAAT ATTATGCCAG TGAAGGACAA AATCCTTTGC CATTTGCTCT TGCGGAATTA 
ATTGACAATT CATTGTCTGC TACTTCTCGT AACATTGGGG TTAGAAGAAT ACAGATCAAA 
TTGCTTTTTG ATGAAACACA AGGAAAACCT GCTGTTGCAG TGATAGATAA TGGAAGAGGA 
ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT CACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGTCCAG TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAAG CTGTCTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAAGATG TTCACGAGCT TGTGCTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCAA TATATAGTGG ATATATTAGA 
AACAGAAAGC CCTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCATAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCACCTTT GGACACGACA GTTAGCGCAT 
ATTTATCACT ACTATATTCA TGGCCCAAAA GGAAATGAAA TACGAACATC AAAAGAAGTT 
GAACCTTTCA ACAATATTGA TATTGAAATT TCTATGTTTG AAAAAGGGAA GGTACCTAAG 
ATTGTCAACC TAAGGGAAAT ACAAGACGAC ATGCAGACGT TGTATGTAAA CACAGCAGCT 
GATAGTTTTG AATTCAAAGC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGT 
TATCATCCAT TCTTATATGA TAGAGAAACT TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA AGATGATTGT TTCATACTTG AGAAAGCAGC TAGAGGGAAA 
AGGCCTATTT TTGAATGTTT TTGGAATGGA CGATTAATAC CATATACATC AGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CAATTGAATG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ATTGACGTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACCCTTT TTACAAGGAT TTTAAATGGA 
CAGGAACAGC GAATGAAAAT TGACAGAGAA TTTGCTTTGT GGCTGAAGGA CTGTCATGAG 
AAGTATGATA AACAAATAAA ATTTACACTT TTTAAGGGAG TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAAGGTCC CTGGGCAACA TATGCAGCAA TAGAATGGGA TGGAAAGATA 
TACAAAGCAG GACAGCTGGT CAAGACAATC AAGACACTTC CCCTCTTTTA TGGAAGCATA 
GTAAGATTTT TTCTTTATGG CGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA GGCACTATAT GATGAAGTAA GAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTT AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGATG AATTATTGCC TAATGAGGTT 
AGGCCTGCTG GAACCCCTAT TGGTGCGTTA AGAATTGAAA TACTGAATAA AAAAGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGCCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATTT TACATTCTTC AAGTGGAAAT AAAGAGATTA TTTCGCATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAGAAGTT GGGGAATTAT 
ACCTTGAAAT TACAAGTTGT GTTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA GTTTTCTGTT AAAGAGGGTA AGCCAGAGAA ATTTTCATTT 
GGTCTTCTGG ATCTTCCTTT TCGTGTTGGA GTTCCATTTA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACCAG TCAACTAGTA ACTGATATTC AGCCAGTTCT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AAAGGACCAA ATTGTGTAAT TCGAGGTGTT 
ACAGCCAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT ATAATCTGAA GGTTACTCTG 
CCTGGCTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTACCTGG TCACCCTCGT 
CGACTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTGG AAGTTTTAGA TGAATCAGAC AACATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTATG TTGTAGATTG CAGTAGTTCT 
GGAACCAGTA TTTTAACAGG ATCTGCAATT CAAGTTCAGA ATATTAAAAA AGACCAGACG 
CTTAAAGCAA GAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA CTACAAATAT TCAGTGTAGA AGGACAAAAG 
GCAATTCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CGGGTGATAT TATGCATAAT 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCA ATATAACATC AGCTTTAGCA 
GAAAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AACACTTGCT ACAGGGTCTG 
CTTCCTGATG TGCAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCG TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATTTAA AATGTGAAAT GAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAGTAG TTATAATAAT TACAGATCAG TACGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTTTAT CTTCTTTGTC AATTGCTGGG GTTGGACTTG ATAGCTCAAA TTTGAAAACA 
ACCTTTCAGG AAAACACACA GAGTATAAGT GTAAGAGGCA TCAAATTTAT TCCAGGTCCT 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAA 
CTAATTTCTG GACCTCCTGC TAAACTTCTC CTTATAGACT GGCCAGAACT AAAGGAGTCC 
ATTCCAGTGA TTAATGGAAG AGATTTACAG AACCCTATTA TTGTTCAACT TTGTGATCAG 
TGGGATAATC CAGCACCGGT ACAACATGTT AAAATAAGTC TTACAAAAGC TAGCAATTTA 
AAGCTCATGC CTTCAAACCA ACAGCATAAA ACAGATGAGA AAGGCAGGGC TAATTTGGGA 
GTATTCAGTG TTTTTGCCCC TAGGGGAGAG CATACTCTTC AGGTTAAAGC CATCTATAAC 
AAAAGTATCA TAGAAGGACC TATAATTAAG TTAATGATTC TTCCAGACCC AGAAAAACCC 
GTTCGTCTCA ATGTTAAATA TGACAAAGAT GCATCCTTCT TAGCAGGGGG TCTTTTCACT 
GATTTTATGA TTAGTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAACAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAGCTGTCT ACCAGTGGGA ACCGACCCCC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAAGAT AATGACAAAG AAGATGGCTG CTTCTATTTC 
AGGGATAAAG TAATTCCTAA TAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAACAA ATATTCTCAA CAGTGAACAG GTTATAGTTG AAGTCCTGCC TAATCAACCT 
GTGAAGTTAG TACCTAAAAT TAAACCACCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAG AGATCTACAT CTTAGTATCA CGGATGACTA CGACAACCAT 
ACTGGAATTG ATTTGGTTGG CACTATAATA GCCACCATTA AAGGCTCTAA TGAGGAAGAT 
ACTGATACCC CACTTTTTAT TGGGAAAGTT AGAACACTTG AATTCCCCTT CGTGAATGGT 
TCGGCTGAAA TCATGAGTCT GGTGCTGGCA GAAAGTAGTC CTGGAAGGGA TAGTACTGAA 
TATTTTATTG TATTTGAGCC CCGGCTACCA CTTTTATCAA GAACCTTAGA ACCATATATC 
CTACCGTTCA TGTTTTACAA TGATGTTAAG AAGCAGCAAC AAATGGCAGC ACTTACAAAA 
GAAAAGGACC AATTATCTCA GTCTATTGTT ATGTATAAAA GTTTATTTGA AGCCAGCCAA 
CAGCTTCTTA ATGAAATGAA ATGTCAAGTT GAAGAAGCAA GATTAAAAGA GGCCCAATTG 
CGAAATGAAC TAAAAATACA TAATATTGAC ATTCCTACAA CACAACAGGT GCCACACATT 
GAAGCACTTC TGAAAAGAAA GCTATCAGAA CAAGAAGAAC TGAAGAAAAA ACCTAGAAGA 
TCGTGTACTC TTCCAAACTA TACTAAAGGC AGTGGAGATG TTTTGGGAAA GATTGCACAT 
CTAGCACAAA TTGAAGATGA TAGAGCTGCG ATGGTTATTT CTTGGCATCT GGCAAGTGAC 
ATGGACTGTG TAGTCACCCT AACCACTGAC GCTGCACGTC GTATCTATGA TGAAACCCAA 
GGTCGTCAGC AGGTGTTGCC CCTTGATTCT ATTTACAAGA AGACTCTTCC AGATTGGAAA 
AGATCTCTAC CTCATTTCCG AAATGGAAAA TTGTATTTTA AACCCATTGG AGATCCAGTC 
TTTGCTCGAG ACTTGTTAAC ATTTCCAGAT AATGTAGAAC ATTGTGAAAC AGTATTTGGT 
ATGCTGTTAG GAGACACCAT TATTTTGGAT AATCTGGATG CGGCCAATCA TTATAGAAAA 
GAGGTTGTTA AAATTACACA CTGTCCTACA CTGCTGACCA GAGATGGAGA TCGAATTCGA 
AGTAATGGAA AGTTTGGGGG CCTTCAGAAT AAAGCTCCTC CAATGGATAA ACTTCGGGGA 
ATGGTATTTG GAGCTCCAGT TCCAAAACAG TGTCTGATCT TAGGGGAACA AATAGATCTT 
CTTCAGCAGT ATCGTTCTGC TGTGTGCAAA CTAGACAGTG TGAATAAGGA TCTTAACAGT 
CAATTAGAGT ACCTTCGCAC TCCGGATATG AGGAAGAAAA AGCAAGAACT TGATGAACAT 
GAGAAAAATC TCAAACTAAT AGAGGAAAAA CTAGGTATGA CTCCCATACG TAAGTGTAAT 
GACTCATTGC GTCATTCACC AAAGGTTGAG ACGACAGATT GTCCAGTTCC TCCTAAAAGA 
ATGAGACGAG AAGCTACAAG ACAAAATAGT CTTCACAATT CAGAAGACAG CAAATCTCAA 
TTAGGACTAG CTACGTTGCA AGTACTCTGG AGCCACACAC ATGGCTGGTG GCTACCGTTT 
TGGACTGCAC AGGATTATAA CCAAAACAGA TGTATGAGAG GTGACAGAGA GAAGAGGCCA 
TTGGTCTCAG TAAGAATGCC CTGCTTTCTG CATCTCTGTT TCAGAAGACC AAGAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_017025684.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881173.1 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.

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CloneID	OHu66025
Clone ID Related Accession (Same CDS sequence)	XM_017025684.1
Accession Version	XM_017025684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT CACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGTCCAG TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAAG CTGTCTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAAGATG TTCACGAGCT TGTGCTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCAA TATATAGTGG ATATATTAGA 
AACAGAAAGC CCTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCATAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCACCTTT GGACACGACA GTTAGCGCAT 
ATTTATCACT ACTATATTCA TGGCCCAAAA GGAAATGAAA TACGAACATC AAAAGAAGTT 
GAACCTTTCA ACAATATTGA TATTGAAATT TCTATGTTTG AAAAAGGGAA GGTACCTAAG 
ATTGTCAACC TAAGGGAAAT ACAAGACGAC ATGCAGACGT TGTATGTAAA CACAGCAGCT 
GATAGTTTTG AATTCAAAGC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGT 
TATCATCCAT TCTTATATGA TAGAGAAACT TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA AGATGATTGT TTCATACTTG AGAAAGCAGC TAGAGGGAAA 
AGGCCTATTT TTGAATGTTT TTGGAATGGA CGATTAATAC CATATACATC AGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CAATTGAATG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ATTGACGTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACCCTTT TTACAAGGAT TTTAAATGGA 
CAGGAACAGC GAATGAAAAT TGACAGAGAA TTTGCTTTGT GGCTGAAGGA CTGTCATGAG 
AAGTATGATA AACAAATAAA ATTTACACTT TTTAAGGGAG TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAAGGTCC CTGGGCAACA TATGCAGCAA TAGAATGGGA TGGAAAGATA 
TACAAAGCAG GACAGCTGGT CAAGACAATC AAGACACTTC CCCTCTTTTA TGGAAGCATA 
GTAAGATTTT TTCTTTATGG CGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA GGCACTATAT GATGAAGTAA GAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTT AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGATG AATTATTGCC TAATGAGGTT 
AGGCCTGCTG GAACCCCTAT TGGTGCGTTA AGAATTGAAA TACTGAATAA AAAAGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGCCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATTT TACATTCTTC AAGTGGAAAT AAAGAGATTA TTTCGCATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAGAAGTT GGGGAATTAT 
ACCTTGAAAT TACAAGTTGT GTTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA GTTTTCTGTT AAAGAGGGTA AGCCAGAGAA ATTTTCATTT 
GGTCTTCTGG ATCTTCCTTT TCGTGTTGGA GTTCCATTTA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACCAG TCAACTAGTA ACTGATATTC AGCCAGTTCT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AAAGGACCAA ATTGTGTAAT TCGAGGTGTT 
ACAGCCAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT ATAATCTGAA GGTTACTCTG 
CCTGGCTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTACCTGG TCACCCTCGT 
CGACTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTGG AAGTTTTAGA TGAATCAGAC AACATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTATG TTGTAGATTG CAGTAGTTCT 
GGAACCAGTA TTTTAACAGG ATCTGCAATT CAAGTTCAGA ATATTAAAAA AGACCAGACG 
CTTAAAGCAA GAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA CTACAAATAT TCAGTGTAGA AGGACAAAAG 
GCAATTCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CGGGTGATAT TATGCATAAT 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCA ATATAACATC AGCTTTAGCA 
GAAAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AACACTTGCT ACAGGGTCTG 
CTTCCTGATG TGCAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCG TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATTTAA AATGTGAAAT GAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAGTAG TTATAATAAT TACAGATCAG TACGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTTTAT CTTCTTTGTC AATTGCTGGG GTTGGACTTG ATAGCTCAAA TTTGAAAACA 
ACCTTTCAGG AAAACACACA GAGTATAAGT GTAAGAGGCA TCAAATTTAT TCCAGGTCCT 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAA 
CTAATTTCTG GACCTCCTGC TAAACTTCTC CTTATAGACT GGCCAGAACT AAAGGAGTCC 
ATTCCAGTGA TTAATGGAAG AGATTTACAG AACCCTATTA TTGTTCAACT TTGTGATCAG 
TGGGATAATC CAGCACCGGT ACAACATGTT AAAATAAGTC TTACAAAAGC TAGCAATTTA 
AAGCTCATGC CTTCAAACCA ACAGCATAAA ACAGATGAGA AAGGCAGGGC TAATTTGGGA 
GTATTCAGTG TTTTTGCCCC TAGGGGAGAG CATACTCTTC AGGTTAAAGC CATCTATAAC 
AAAAGTATCA TAGAAGGACC TATAATTAAG TTAATGATTC TTCCAGACCC AGAAAAACCC 
GTTCGTCTCA ATGTTAAATA TGACAAAGAT GCATCCTTCT TAGCAGGGGG TCTTTTCACT 
GATTTTATGA TTAGTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAACAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAGCTGTCT ACCAGTGGGA ACCGACCCCC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAAGAT AATGACAAAG AAGATGGCTG CTTCTATTTC 
AGGGATAAAG TAATTCCTAA TAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAACAA ATATTCTCAA CAGTGAACAG GTTATAGTTG AAGTCCTGCC TAATCAACCT 
GTGAAGTTAG TACCTAAAAT TAAACCACCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAG AGATCTACAT CTTAGTATCA CGGATGACTA CGACAACCAT 
ACTGGAATTG ATTTGGTTGG CACTATAATA GCCACCATTA AAGGCTCTAA TGAGGAAGAT 
ACTGATACCC CACTTTTTAT TGGGAAAGTT AGAACACTTG AATTCCCCTT CGTGAATGGT 
TCGGCTGAAA TCATGAGTCT GGTGCTGGCA GAAAGTAGTC CTGGAAGGGA TAGTACTGAA 
TATTTTATTG TATTTGAGCC CCGGCTACCA CTTTTATCAA GAACCTTAGA ACCATATATC 
CTACCGTTCA TGTTTTACAA TGATGTTAAG AAGCAGCAAC AAATGGCAGC ACTTACAAAA 
GAAAAGGACC AATTATCTCA GTCTATTGTT ATGTATAAAA GTTTATTTGA AGCCAGCCAA 
CAGCTTCTTA ATGAAATGAA ATGTCAAGTT GAAGAAGCAA GATTAAAAGA GGCCCAATTG 
CGAAATGAAC TAAAAATACA TAATATTGAC ATTCCTACAA CACAACAGGT GCCACACATT 
GAAGCACTTC TGAAAAGAAA GCTATCAGAA CAAGAAGAAC TGAAGAAAAA ACCTAGAAGA 
TCGTGTACTC TTCCAAACTA TACTAAAGGC AGTGGAGATG TTTTGGGAAA GATTGCACAT 
CTAGCACAAA TTGAAGATGA TAGAGCTGCG ATGGTTATTT CTTGGCATCT GGCAAGTGAC 
ATGGACTGTG TAGTCACCCT AACCACTGAC GCTGCACGTC GTATCTATGA TGAAACCCAA 
GGTCGTCAGC AGGTGTTGCC CCTTGATTCT ATTTACAAGA AGACTCTTCC AGATTGGAAA 
AGATCTCTAC CTCATTTCCG AAATGGAAAA TTGTATTTTA AACCCATTGG AGATCCAGTC 
TTTGCTCGAG ACTTGTTAAC ATTTCCAGAT AATGTAGAAC ATTGTGAAAC AGTATTTGGT 
ATGCTGTTAG GAGACACCAT TATTTTGGAT AATCTGGATG CGGCCAATCA TTATAGAAAA 
GAGGTTGTTA AAATTACACA CTGTCCTACA CTGCTGACCA GAGATGGAGA TCGAATTCGA 
AGTAATGGAA AGTTTGGGGG CCTTCAGAAT AAAGCTCCTC CAATGGATAA ACTTCGGGGA 
ATGGTATTTG GAGCTCCAGT TCCAAAACAG TGTCTGATCT TAGGGGAACA AATAGATCTT 
CTTCAGCAGT ATCGTTCTGC TGTGTGCAAA CTAGACAGTG TGAATAAGGA TCTTAACAGT 
CAATTAGAGT ACCTTCGCAC TCCGGATATG AGGAAGAAAA AGCAAGAACT TGATGAACAT 
GAGAAAAATC TCAAACTAAT AGAGGAAAAA CTAGGTATGA CTCCCATACG TAAGTGTAAT 
GACTCATTGC GTCATTCACC AAAGGTTGAG ACGACAGATT GTCCAGTTCC TCCTAAAAGA 
ATGAGACGAG AAGCTACAAG ACAAAATAGT CTTCACAATT CAGAAGACAG CAAATCTCAA 
TTAGGACTAG CTACGTTGCA AGTACTCTGG AGCCACACAC ATGGCTGGTG GCTACCGTTT 
TGGACTGCAC AGGATTATAA CCAAAACAGA TGTATGAGAG GTGACAGAGA GAAGAGGCCA 
TTGGTCTCAG TAAGAATGCC CTGCTTTCTG CATCTCTGTT TCAGAAGACC AAGAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881173.1
CDS	932..6571
Translation	MTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNSLHNSEDSKSQLGLATLQVLWSHTHGWWLPFWTAQDYNQNRCMRGDREKRPLVSVRMPCFLHLCFRRPRG

Target ORF information:

RefSeq Version	XM_017025684.1
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025684.1

ORF Insert Sequence:

ATGACCTCTA AACAGCTTAA CAACTGGGCC GTGTATAGGT TGTCAAAATT CACAAGGCAA 
GGTGACTTTG AAAGTGATCA TTCAGGATAT GTTCGTCCAG TACCAGTGCC ACGCAGTTTA 
AATAGTGATA TTTCCTATTT TGGTGTTGGG GGCAAGCAAG CTGTCTTCTT TGTTGGACAA 
TCAGCCAGAA TGATAAGCAA ACCTGCAGAT TCCCAAGATG TTCACGAGCT TGTGCTTTCT 
AAAGAAGATT TTGAGAAGAA GGAGAAAAAT AAAGAGGCAA TATATAGTGG ATATATTAGA 
AACAGAAAGC CCTCTGATTC TGTTCACATT ACAAATGATG ATGAAAGATT TCTACATCAT 
CTTATCATAG AGGAGAAGGA AAAAGATAGC TTTACTGCTG TGGTTATCAC AGGGGTACAA 
CCAGAACACA TACAGTACTT GAAAAATTAT TTCCACCTTT GGACACGACA GTTAGCGCAT 
ATTTATCACT ACTATATTCA TGGCCCAAAA GGAAATGAAA TACGAACATC AAAAGAAGTT 
GAACCTTTCA ACAATATTGA TATTGAAATT TCTATGTTTG AAAAAGGGAA GGTACCTAAG 
ATTGTCAACC TAAGGGAAAT ACAAGACGAC ATGCAGACGT TGTATGTAAA CACAGCAGCT 
GATAGTTTTG AATTCAAAGC TCATGTTGAA GGAGATGGTG TAGTGGAAGG GATTATCCGT 
TATCATCCAT TCTTATATGA TAGAGAAACT TACCCTGATG ATCCATGCTT TCCATCAAAA 
TTAAAAGATG AAGATGATGA AGATGATTGT TTCATACTTG AGAAAGCAGC TAGAGGGAAA 
AGGCCTATTT TTGAATGTTT TTGGAATGGA CGATTAATAC CATATACATC AGTTGAAGAC 
TTTGACTGGT GTACTCCTCC TAAGAAGAGA GGGCTTGCAC CAATTGAATG CTACAATAGG 
ATATCTGGTG CATTATTCAC TAATGACAAA TTCCAGGTCA GCACAAATAA ATTGACGTTT 
ATGGATCTTG AGCTAAAATT GAAAGATAAG AACACCCTTT TTACAAGGAT TTTAAATGGA 
CAGGAACAGC GAATGAAAAT TGACAGAGAA TTTGCTTTGT GGCTGAAGGA CTGTCATGAG 
AAGTATGATA AACAAATAAA ATTTACACTT TTTAAGGGAG TAATTACACG TCCTGATCTT 
CCTTCTAAAA AGCAAGGTCC CTGGGCAACA TATGCAGCAA TAGAATGGGA TGGAAAGATA 
TACAAAGCAG GACAGCTGGT CAAGACAATC AAGACACTTC CCCTCTTTTA TGGAAGCATA 
GTAAGATTTT TTCTTTATGG CGATCATGAT GGAGAAGTAT ATGCTACAGG AGGAGAGGTT 
CAAATTGCAA TGGAACCTCA GGCACTATAT GATGAAGTAA GAACTGTGCC AATTGCAAAG 
CTGGATAGGA CAGTTGCTGA GAAAGCTGTT AAAAAATATG TAGAAGATGA AATGGCAAGG 
CTCCCTGATA GATTGTCAGT AACTTGGCCT GAAGGAGATG AATTATTGCC TAATGAGGTT 
AGGCCTGCTG GAACCCCTAT TGGTGCGTTA AGAATTGAAA TACTGAATAA AAAAGGGGAA 
GCAATGCAAA AGCTTCCAGG AACAAGCCAT GGAGGGTCAA AGAAACTCCT GGTTGAGCTC 
AAAGTTATTT TACATTCTTC AAGTGGAAAT AAAGAGATTA TTTCGCATAT TAGTCAACAT 
GGAGGAAAAT GGCCTTACTG GTTTAAAAAA ATGGAAAATA TTCAGAAGTT GGGGAATTAT 
ACCTTGAAAT TACAAGTTGT GTTGAATGAA AGTAATGCAG ACACTTATGC AGGAAGACCA 
CTACCATCTA AAGCAATTAA GTTTTCTGTT AAAGAGGGTA AGCCAGAGAA ATTTTCATTT 
GGTCTTCTGG ATCTTCCTTT TCGTGTTGGA GTTCCATTTA ATATCCCTCT GGAGTTTCAG 
GATGAATTTG GTCATACCAG TCAACTAGTA ACTGATATTC AGCCAGTTCT TGAAGCAAGT 
GGTTTATCTT TACATTATGA AGAAATAACC AAAGGACCAA ATTGTGTAAT TCGAGGTGTT 
ACAGCCAAGG GCCCTGTAAA CTCTTGTCAA GGCAAGAATT ATAATCTGAA GGTTACTCTG 
CCTGGCTTAA AAGAAGACTC ACAGATTTTG AAAATTAGAT TACTACCTGG TCACCCTCGT 
CGACTGAAAG TGAAACCTGA TTCTGAAATT TTAGTTATAG AAAATGGAAC AGCTTTCCCA 
TTTCAGGTGG AAGTTTTAGA TGAATCAGAC AACATAACAG CACAACCAAA ATTGATTGTT 
CATTGTAAGT TTTCAGGTGC TCCAAACCTT CCAGTCTATG TTGTAGATTG CAGTAGTTCT 
GGAACCAGTA TTTTAACAGG ATCTGCAATT CAAGTTCAGA ATATTAAAAA AGACCAGACG 
CTTAAAGCAA GAATTGAAAT ACCTAGTTGT AAAGATGTGG CACCTGTGGA GAAGACTATT 
AAGTTGCTTC CCAGTAGCCA TGTTGCAAGA CTACAAATAT TCAGTGTAGA AGGACAAAAG 
GCAATTCAGA TCAAACATCA GGATGAGGTT AATTGGATAG CGGGTGATAT TATGCATAAT 
CTTATTTTTC AAATGTATGA TGAAGGAGAA AGAGAAATCA ATATAACATC AGCTTTAGCA 
GAAAAAATTA AAGTTAATTG GACTCCTGAG ATTAACAAAG AACACTTGCT ACAGGGTCTG 
CTTCCTGATG TGCAAGTACC AACATCTGTA AAAGATATGC GCTATTGCCA GGTTTCATTC 
CAAGATGATC ATGTGTCTTT GGAAAGTGCG TTTACAGTAA GACCACTTCC TGATGAACCT 
AAACATTTAA AATGTGAAAT GAAAGGAGGA AAAACAGTAC AGATGGGCCA AGAGCTTCAA 
GGAGAAGTAG TTATAATAAT TACAGATCAG TACGGAAATC AGATTCAAGC ATTTTCACCA 
AGTTCTTTAT CTTCTTTGTC AATTGCTGGG GTTGGACTTG ATAGCTCAAA TTTGAAAACA 
ACCTTTCAGG AAAACACACA GAGTATAAGT GTAAGAGGCA TCAAATTTAT TCCAGGTCCT 
CCTGGAAATA AGGATCTTTG TTTTACTTGG CGTGAGTTTT CTGACTTTAT TCGAGTGCAA 
CTAATTTCTG GACCTCCTGC TAAACTTCTC CTTATAGACT GGCCAGAACT AAAGGAGTCC 
ATTCCAGTGA TTAATGGAAG AGATTTACAG AACCCTATTA TTGTTCAACT TTGTGATCAG 
TGGGATAATC CAGCACCGGT ACAACATGTT AAAATAAGTC TTACAAAAGC TAGCAATTTA 
AAGCTCATGC CTTCAAACCA ACAGCATAAA ACAGATGAGA AAGGCAGGGC TAATTTGGGA 
GTATTCAGTG TTTTTGCCCC TAGGGGAGAG CATACTCTTC AGGTTAAAGC CATCTATAAC 
AAAAGTATCA TAGAAGGACC TATAATTAAG TTAATGATTC TTCCAGACCC AGAAAAACCC 
GTTCGTCTCA ATGTTAAATA TGACAAAGAT GCATCCTTCT TAGCAGGGGG TCTTTTCACT 
GATTTTATGA TTAGTGTTAT TTCTGAAGAT GACAGTATCA TTAAAAACAT TAATCCAGCA 
CGTATTTCCA TGAAAATGTG GAAGCTGTCT ACCAGTGGGA ACCGACCCCC AGCAAATGCA 
GAAACATTTA GTTGTAATAA AATAAAAGAT AATGACAAAG AAGATGGCTG CTTCTATTTC 
AGGGATAAAG TAATTCCTAA TAAAGTGGGG ACATATTGTA TCCAGTTTGG TTTTATGATG 
GATAAAACAA ATATTCTCAA CAGTGAACAG GTTATAGTTG AAGTCCTGCC TAATCAACCT 
GTGAAGTTAG TACCTAAAAT TAAACCACCT ACACCAGCTG TTTCAAATGT TCGCTCAGTT 
GCCAGTAGGA CCTTGGTCAG AGATCTACAT CTTAGTATCA CGGATGACTA CGACAACCAT 
ACTGGAATTG ATTTGGTTGG CACTATAATA GCCACCATTA AAGGCTCTAA TGAGGAAGAT 
ACTGATACCC CACTTTTTAT TGGGAAAGTT AGAACACTTG AATTCCCCTT CGTGAATGGT 
TCGGCTGAAA TCATGAGTCT GGTGCTGGCA GAAAGTAGTC CTGGAAGGGA TAGTACTGAA 
TATTTTATTG TATTTGAGCC CCGGCTACCA CTTTTATCAA GAACCTTAGA ACCATATATC 
CTACCGTTCA TGTTTTACAA TGATGTTAAG AAGCAGCAAC AAATGGCAGC ACTTACAAAA 
GAAAAGGACC AATTATCTCA GTCTATTGTT ATGTATAAAA GTTTATTTGA AGCCAGCCAA 
CAGCTTCTTA ATGAAATGAA ATGTCAAGTT GAAGAAGCAA GATTAAAAGA GGCCCAATTG 
CGAAATGAAC TAAAAATACA TAATATTGAC ATTCCTACAA CACAACAGGT GCCACACATT 
GAAGCACTTC TGAAAAGAAA GCTATCAGAA CAAGAAGAAC TGAAGAAAAA ACCTAGAAGA 
TCGTGTACTC TTCCAAACTA TACTAAAGGC AGTGGAGATG TTTTGGGAAA GATTGCACAT 
CTAGCACAAA TTGAAGATGA TAGAGCTGCG ATGGTTATTT CTTGGCATCT GGCAAGTGAC 
ATGGACTGTG TAGTCACCCT AACCACTGAC GCTGCACGTC GTATCTATGA TGAAACCCAA 
GGTCGTCAGC AGGTGTTGCC CCTTGATTCT ATTTACAAGA AGACTCTTCC AGATTGGAAA 
AGATCTCTAC CTCATTTCCG AAATGGAAAA TTGTATTTTA AACCCATTGG AGATCCAGTC 
TTTGCTCGAG ACTTGTTAAC ATTTCCAGAT AATGTAGAAC ATTGTGAAAC AGTATTTGGT 
ATGCTGTTAG GAGACACCAT TATTTTGGAT AATCTGGATG CGGCCAATCA TTATAGAAAA 
GAGGTTGTTA AAATTACACA CTGTCCTACA CTGCTGACCA GAGATGGAGA TCGAATTCGA 
AGTAATGGAA AGTTTGGGGG CCTTCAGAAT AAAGCTCCTC CAATGGATAA ACTTCGGGGA 
ATGGTATTTG GAGCTCCAGT TCCAAAACAG TGTCTGATCT TAGGGGAACA AATAGATCTT 
CTTCAGCAGT ATCGTTCTGC TGTGTGCAAA CTAGACAGTG TGAATAAGGA TCTTAACAGT 
CAATTAGAGT ACCTTCGCAC TCCGGATATG AGGAAGAAAA AGCAAGAACT TGATGAACAT 
GAGAAAAATC TCAAACTAAT AGAGGAAAAA CTAGGTATGA CTCCCATACG TAAGTGTAAT 
GACTCATTGC GTCATTCACC AAAGGTTGAG ACGACAGATT GTCCAGTTCC TCCTAAAAGA 
ATGAGACGAG AAGCTACAAG ACAAAATAGT CTTCACAATT CAGAAGACAG CAAATCTCAA 
TTAGGACTAG CTACGTTGCA AGTACTCTGG AGCCACACAC ATGGCTGGTG GCTACCGTTT 
TGGACTGCAC AGGATTATAA CCAAAACAGA TGTATGAGAG GTGACAGAGA GAAGAGGCCA 
TTGGTCTCAG TAAGAATGCC CTGCTTTCTG CATCTCTGTT TCAGAAGACC AAGAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_011525642.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523944.1 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

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CloneID	OHu66022
Clone ID Related Accession (Same CDS sequence)	XM_011525642.1
Accession Version	XM_011525642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGTCTTCAC 
AATTCAGAAG ACAGCAAATC TCAATTAGGA CTAGCTACGT TGCAAGTACT CTGGAGCCAC 
ACACATGGCT GGTGGCTACC GTTTTGGACT GCACAGGATT ATAACCAAAA CAGATGTATG 
AGAGGTGACA GAGAGAAGAG GCCATTGGTC TCAGTAAGAA TGCCCTGCTT TCTGCATCTC 
TGTTTCAGAA GACCAAGAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523944.1
CDS	190..6393
Translation	MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPLQVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNSLHNSEDSKSQLGLATLQVLWSHTHGWWLPFWTAQDYNQNRCMRGDREKRPLVSVRMPCFLHLCFRRPRG

Target ORF information:

RefSeq Version	XM_011525642.1
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525642.1

ORF Insert Sequence:

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGTCTTCAC 
AATTCAGAAG ACAGCAAATC TCAATTAGGA CTAGCTACGT TGCAAGTACT CTGGAGCCAC 
ACACATGGCT GGTGGCTACC GTTTTGGACT GCACAGGATT ATAACCAAAA CAGATGTATG 
AGAGGTGACA GAGAGAAGAG GCCATTGGTC TCAGTAAGAA TGCCCTGCTT TCTGCATCTC 
TGTTTCAGAA GACCAAGAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_011525643.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523945.1 Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.

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CloneID	OHu66023
Clone ID Related Accession (Same CDS sequence)	XM_011525643.2
Accession Version	XM_011525643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGAGATGGG 
GTCTTGCTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523945.1
CDS	190..6204
Translation	MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRPLQVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNIGVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNLREIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQLVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKEDSQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKFSGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSSNLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTDEKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKDASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPKIKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTPLFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILPFMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQLRNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRDGVLLC

Target ORF information:

RefSeq Version	XM_011525643.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525643.2

ORF Insert Sequence:

ATGGCAGCGG CGGACGGCGG CGGGCCTGGT GGGGCCTCTG TGGGGACTGA GGAGGATGGC 
GGAGGCGTCG GCCACAGGAC GGTGTACTTG TTTGATCGGC GCGAAAAGGA GTCCGAGCTC 
GGGGACCGGC CTCTGCAGGT CGGGGAGCGC TCGGACTACG CGGGATTTCG CGCGTGTGTG 
TGTCAGACAC TTGGCATTTC ACCTGAAGAA AAATTTGTTA TTACAACAAC AAGTAGGAAA 
GAAATTACCT GTGATAATTT TGATGAAACT GTTAAAGATG GAGTCACCTT ATACCTGCTA 
CAGTCGGTCA ATCAGTTACT ACTGACAGCT ACGAAAGAAC GAATTGACTT CTTACCTCAC 
TATGACACAC TGGTTAAAAG TGGCATGTAT GAATATTATG CCAGTGAAGG ACAAAATCCT 
TTGCCATTTG CTCTTGCGGA ATTAATTGAC AATTCATTGT CTGCTACTTC TCGTAACATT 
GGGGTTAGAA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTT 
GCAGTGATAG ATAATGGAAG AGGAATGACC TCTAAACAGC TTAACAACTG GGCCGTGTAT 
AGGTTGTCAA AATTCACAAG GCAAGGTGAC TTTGAAAGTG ATCATTCAGG ATATGTTCGT 
CCAGTACCAG TGCCACGCAG TTTAAATAGT GATATTTCCT ATTTTGGTGT TGGGGGCAAG 
CAAGCTGTCT TCTTTGTTGG ACAATCAGCC AGAATGATAA GCAAACCTGC AGATTCCCAA 
GATGTTCACG AGCTTGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAATAAAGAG 
GCAATATATA GTGGATATAT TAGAAACAGA AAGCCCTCTG ATTCTGTTCA CATTACAAAT 
GATGATGAAA GATTTCTACA TCATCTTATC ATAGAGGAGA AGGAAAAAGA TAGCTTTACT 
GCTGTGGTTA TCACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTATTTCCAC 
CTTTGGACAC GACAGTTAGC GCATATTTAT CACTACTATA TTCATGGCCC AAAAGGAAAT 
GAAATACGAA CATCAAAAGA AGTTGAACCT TTCAACAATA TTGATATTGA AATTTCTATG 
TTTGAAAAAG GGAAGGTACC TAAGATTGTC AACCTAAGGG AAATACAAGA CGACATGCAG 
ACGTTGTATG TAAACACAGC AGCTGATAGT TTTGAATTCA AAGCTCATGT TGAAGGAGAT 
GGTGTAGTGG AAGGGATTAT CCGTTATCAT CCATTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAATTAAAA GATGAAGATG ATGAAGATGA TTGTTTCATA 
CTTGAGAAAG CAGCTAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGATTA 
ATACCATATA CATCAGTTGA AGACTTTGAC TGGTGTACTC CTCCTAAGAA GAGAGGGCTT 
GCACCAATTG AATGCTACAA TAGGATATCT GGTGCATTAT TCACTAATGA CAAATTCCAG 
GTCAGCACAA ATAAATTGAC GTTTATGGAT CTTGAGCTAA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GGATTTTAAA TGGACAGGAA CAGCGAATGA AAATTGACAG AGAATTTGCT 
TTGTGGCTGA AGGACTGTCA TGAGAAGTAT GATAAACAAA TAAAATTTAC ACTTTTTAAG 
GGAGTAATTA CACGTCCTGA TCTTCCTTCT AAAAAGCAAG GTCCCTGGGC AACATATGCA 
GCAATAGAAT GGGATGGAAA GATATACAAA GCAGGACAGC TGGTCAAGAC AATCAAGACA 
CTTCCCCTCT TTTATGGAAG CATAGTAAGA TTTTTTCTTT ATGGCGATCA TGATGGAGAA 
GTATATGCTA CAGGAGGAGA GGTTCAAATT GCAATGGAAC CTCAGGCACT ATATGATGAA 
GTAAGAACTG TGCCAATTGC AAAGCTGGAT AGGACAGTTG CTGAGAAAGC TGTTAAAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CAGTAACTTG GCCTGAAGGA 
GATGAATTAT TGCCTAATGA GGTTAGGCCT GCTGGAACCC CTATTGGTGC GTTAAGAATT 
GAAATACTGA ATAAAAAAGG GGAAGCAATG CAAAAGCTTC CAGGAACAAG CCATGGAGGG 
TCAAAGAAAC TCCTGGTTGA GCTCAAAGTT ATTTTACATT CTTCAAGTGG AAATAAAGAG 
ATTATTTCGC ATATTAGTCA ACATGGAGGA AAATGGCCTT ACTGGTTTAA AAAAATGGAA 
AATATTCAGA AGTTGGGGAA TTATACCTTG AAATTACAAG TTGTGTTGAA TGAAAGTAAT 
GCAGACACTT ATGCAGGAAG ACCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGTAAGCCAG AGAAATTTTC ATTTGGTCTT CTGGATCTTC CTTTTCGTGT TGGAGTTCCA 
TTTAATATCC CTCTGGAGTT TCAGGATGAA TTTGGTCATA CCAGTCAACT AGTAACTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTTTA TCTTTACATT ATGAAGAAAT AACCAAAGGA 
CCAAATTGTG TAATTCGAGG TGTTACAGCC AAGGGCCCTG TAAACTCTTG TCAAGGCAAG 
AATTATAATC TGAAGGTTAC TCTGCCTGGC TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTACTAC CTGGTCACCC TCGTCGACTG AAAGTGAAAC CTGATTCTGA AATTTTAGTT 
ATAGAAAATG GAACAGCTTT CCCATTTCAG GTGGAAGTTT TAGATGAATC AGACAACATA 
ACAGCACAAC CAAAATTGAT TGTTCATTGT AAGTTTTCAG GTGCTCCAAA CCTTCCAGTC 
TATGTTGTAG ATTGCAGTAG TTCTGGAACC AGTATTTTAA CAGGATCTGC AATTCAAGTT 
CAGAATATTA AAAAAGACCA GACGCTTAAA GCAAGAATTG AAATACCTAG TTGTAAAGAT 
GTGGCACCTG TGGAGAAGAC TATTAAGTTG CTTCCCAGTA GCCATGTTGC AAGACTACAA 
ATATTCAGTG TAGAAGGACA AAAGGCAATT CAGATCAAAC ATCAGGATGA GGTTAATTGG 
ATAGCGGGTG ATATTATGCA TAATCTTATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATAA CATCAGCTTT AGCAGAAAAA ATTAAAGTTA ATTGGACTCC TGAGATTAAC 
AAAGAACACT TGCTACAGGG TCTGCTTCCT GATGTGCAAG TACCAACATC TGTAAAAGAT 
ATGCGCTATT GCCAGGTTTC ATTCCAAGAT GATCATGTGT CTTTGGAAAG TGCGTTTACA 
GTAAGACCAC TTCCTGATGA ACCTAAACAT TTAAAATGTG AAATGAAAGG AGGAAAAACA 
GTACAGATGG GCCAAGAGCT TCAAGGAGAA GTAGTTATAA TAATTACAGA TCAGTACGGA 
AATCAGATTC AAGCATTTTC ACCAAGTTCT TTATCTTCTT TGTCAATTGC TGGGGTTGGA 
CTTGATAGCT CAAATTTGAA AACAACCTTT CAGGAAAACA CACAGAGTAT AAGTGTAAGA 
GGCATCAAAT TTATTCCAGG TCCTCCTGGA AATAAGGATC TTTGTTTTAC TTGGCGTGAG 
TTTTCTGACT TTATTCGAGT GCAACTAATT TCTGGACCTC CTGCTAAACT TCTCCTTATA 
GACTGGCCAG AACTAAAGGA GTCCATTCCA GTGATTAATG GAAGAGATTT ACAGAACCCT 
ATTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCAGCAC CGGTACAACA TGTTAAAATA 
AGTCTTACAA AAGCTAGCAA TTTAAAGCTC ATGCCTTCAA ACCAACAGCA TAAAACAGAT 
GAGAAAGGCA GGGCTAATTT GGGAGTATTC AGTGTTTTTG CCCCTAGGGG AGAGCATACT 
CTTCAGGTTA AAGCCATCTA TAACAAAAGT ATCATAGAAG GACCTATAAT TAAGTTAATG 
ATTCTTCCAG ACCCAGAAAA ACCCGTTCGT CTCAATGTTA AATATGACAA AGATGCATCC 
TTCTTAGCAG GGGGTCTTTT CACTGATTTT ATGATTAGTG TTATTTCTGA AGATGACAGT 
ATCATTAAAA ACATTAATCC AGCACGTATT TCCATGAAAA TGTGGAAGCT GTCTACCAGT 
GGGAACCGAC CCCCAGCAAA TGCAGAAACA TTTAGTTGTA ATAAAATAAA AGATAATGAC 
AAAGAAGATG GCTGCTTCTA TTTCAGGGAT AAAGTAATTC CTAATAAAGT GGGGACATAT 
TGTATCCAGT TTGGTTTTAT GATGGATAAA ACAAATATTC TCAACAGTGA ACAGGTTATA 
GTTGAAGTCC TGCCTAATCA ACCTGTGAAG TTAGTACCTA AAATTAAACC ACCTACACCA 
GCTGTTTCAA ATGTTCGCTC AGTTGCCAGT AGGACCTTGG TCAGAGATCT ACATCTTAGT 
ATCACGGATG ACTACGACAA CCATACTGGA ATTGATTTGG TTGGCACTAT AATAGCCACC 
ATTAAAGGCT CTAATGAGGA AGATACTGAT ACCCCACTTT TTATTGGGAA AGTTAGAACA 
CTTGAATTCC CCTTCGTGAA TGGTTCGGCT GAAATCATGA GTCTGGTGCT GGCAGAAAGT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTGTATTTG AGCCCCGGCT ACCACTTTTA 
TCAAGAACCT TAGAACCATA TATCCTACCG TTCATGTTTT ACAATGATGT TAAGAAGCAG 
CAACAAATGG CAGCACTTAC AAAAGAAAAG GACCAATTAT CTCAGTCTAT TGTTATGTAT 
AAAAGTTTAT TTGAAGCCAG CCAACAGCTT CTTAATGAAA TGAAATGTCA AGTTGAAGAA 
GCAAGATTAA AAGAGGCCCA ATTGCGAAAT GAACTAAAAA TACATAATAT TGACATTCCT 
ACAACACAAC AGGTGCCACA CATTGAAGCA CTTCTGAAAA GAAAGCTATC AGAACAAGAA 
GAACTGAAGA AAAAACCTAG AAGATCGTGT ACTCTTCCAA ACTATACTAA AGGCAGTGGA 
GATGTTTTGG GAAAGATTGC ACATCTAGCA CAAATTGAAG ATGATAGAGC TGCGATGGTT 
ATTTCTTGGC ATCTGGCAAG TGACATGGAC TGTGTAGTCA CCCTAACCAC TGACGCTGCA 
CGTCGTATCT ATGATGAAAC CCAAGGTCGT CAGCAGGTGT TGCCCCTTGA TTCTATTTAC 
AAGAAGACTC TTCCAGATTG GAAAAGATCT CTACCTCATT TCCGAAATGG AAAATTGTAT 
TTTAAACCCA TTGGAGATCC AGTCTTTGCT CGAGACTTGT TAACATTTCC AGATAATGTA 
GAACATTGTG AAACAGTATT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAATCTG 
GATGCGGCCA ATCATTATAG AAAAGAGGTT GTTAAAATTA CACACTGTCC TACACTGCTG 
ACCAGAGATG GAGATCGAAT TCGAAGTAAT GGAAAGTTTG GGGGCCTTCA GAATAAAGCT 
CCTCCAATGG ATAAACTTCG GGGAATGGTA TTTGGAGCTC CAGTTCCAAA ACAGTGTCTG 
ATCTTAGGGG AACAAATAGA TCTTCTTCAG CAGTATCGTT CTGCTGTGTG CAAACTAGAC 
AGTGTGAATA AGGATCTTAA CAGTCAATTA GAGTACCTTC GCACTCCGGA TATGAGGAAG 
AAAAAGCAAG AACTTGATGA ACATGAGAAA AATCTCAAAC TAATAGAGGA AAAACTAGGT 
ATGACTCCCA TACGTAAGTG TAATGACTCA TTGCGTCATT CACCAAAGGT TGAGACGACA 
GATTGTCCAG TTCCTCCTAA AAGAATGAGA CGAGAAGCTA CAAGACAAAA TAGAGATGGG 
GTCTTGCTGT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SMCHD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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