AFF2 cDNA ORF clone, Homo sapiens(human)

The following AFF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22585 NM_002025.4
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu08710 NM_001170628.1
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $657.30
$939.00
OHu10427 NM_001169125.2
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu11095 NM_001169124.2
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu12312 NM_001169123.2
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu12937 NM_001169122.2
Latest version!
Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $902.30
$1289.00
View Old Accession Versions >>
OHu22585 NM_002025.3 Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu12937 NM_001169122.1 Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $902.30
$1289.00
OHu10427 NM_001169125.1 Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu12312 NM_001169123.1 Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu11095 NM_001169124.1 Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22585
    Clone ID Related Accession (Same CDS sequence) NM_002025.4 , NM_002025.3
    Accession Version NM_002025.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002368.1, BC143744.1, BC132683.1, L76569.1 and AC231841.2. This sequence is a reference standard in the RefSeqGene project. On Mar 20, 2019 this sequence version replaced NM_002025.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370460.7/ ENSP00000359489.2 RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAGA TGACCCATTC CTGGCCTACT CCTCTCACTT CCATGCATAC TGCTGGACAC
    TCTGAGCAGA GCACCTTTTC CATCCCAGGA CAGGAATCGC AGCATCTGAC CCCAGGATTC
    ACCTTACAAA AGTGGAATGA CCCAACCACC AGAGCTTCTA CAAAGTCAGT GTCTTTCAAA
    TCGATGCTTG AGGATGACCT GAAGCTGAGC AGTGATGAAG ATGACCTTGA GCCTGTGAAG
    ACCTTGACCA CTCAGTGCAC TGCCACTGAG CTCTACCAGG CTGTTGAAAA GGCAAAACCT
    AGGAATAATC CTGTGAACCC ACCCTTGGCC ACTCCCCAGC CCCCACCTGC AGTGCAAGCC
    AGCGGGGGTT CTGGCAGCTC CAGCGAATCG GAGAGCAGCT CTGAGTCGGA TTCAGACACT
    GAAAGTAGCA CCACTGACAG CGAATCTAAT GAGGCACCTC GTGTGGCAAC TCCAGAGCCT
    GAGCCACCCT CAACCAACAA GTGGCAACTG GATAAATGGC TTAACAAAGT GACATCCCAG
    AACAAGTCTT TTATTTGTGG CCAAAATGAA ACACCCATGG AGACTATTTC TCTGCCTCCT
    CCAATCATCC AACCAATGGA AGTCCAGATG AAAGTGAAGA CGAATGCCAG TCAGGTCCCA
    GCTGAACCCA AAGAAAGGCC TCTCCTCAGT CTCATTAGGG AGAAAGCCCG TCCACGGCCC
    ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG AAGCATAAGT TGTCAACAAC TAGTGAGACA
    GTGTCTCAAA GGACAATTGG GAAAAAACAG CCCAAAAAAG TTGAGAAGAA CACCAGCACT
    GACGAGTTTA CCTGGCCCAA ACCAAATATT ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT
    GTGGAGCTTC ATGACCCACC AAGAGGCCGC AACAAAGCCA CTGCCCACAA ACCAGCCCCT
    AGGAAAGAAC CAAGACCTAA CATCCCTTTG GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT
    GGCAAGATTG TGCCAAAGTC TCGGGAATTC ATTGAAACAG ATTCATCTAC ATCTGACTCC
    AACACAGATC AGGAAGAGAC CCTGCAAATC AAAGTCCTGC CTCCGTGCAT TATTTCTGGA
    GGTAATACTG CCAAATCCAA GGAAATCTGT GGTGCCAGCC TGACCCTCAG CACCTTAATG
    AGTAGCAGTG GCAGCAACAA CAACTTATCC ATCAGTAATG AAGAGCCAAC ATTTTCACCT
    ATTCCTGTCA TGCAAACTGA AATCCTGTCC CCTCTGCGAG ATCATGAGAA CCTGAAAAAC
    CTCTGGGTGA AGATTGACCT TGACTTACTC TCTAGAGTAC CTGGCCACAG CTCACTCCAT
    GCAGCACCTG CCAAGCCAGA CCACAAGGAG ACTGCCACAA AACCCAAGCG TCAGACAGCT
    GTCACAGCTG TGGAGAAACC AGCCCCTAAG GGCAAACGTA AGCACAAGCC AATAGAAGTT
    GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC CTGGAGGAGG CCACAACTAT CTGCTTGCTC
    CCTCCTTGCA TCTCACCAGC CCCACCCCAC AAGCCTCCCA ACACTAGAGA AAATAATTCA
    TCCAGGAGAG CAAATAGAAG AAAGGAAGAA AAACTATTTC CTCCTCCACT TTCCCCACTG
    CCAGAGGACC CTCCACGCCG CAGAAATGTC AGTGGCAATA ATGGTCCCTT TGGTCAAGAC
    AAAAACATCG CCATGACTGG ACAAATCACA TCTACCAAAC CTAAGAGAAC TGAAGGCAAA
    TTCTGTGCTA CTTTCAAAGG GATATCGGTA AATGAGGGAG ACACTCCAAA AAAGGCATCC
    TCTGCCACCA TCACTGTCAC CAATACTGCT ATTGCCACTG CTACTGTCAC TGCTACTGCC
    ATTGTCACCA CCACTGTCAC AGCTACTGCC ACCGCCACGG CCACCACCAC AACTACTACC
    ACTACCATTT CCACCATCAC CTCTACCATC ACTACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACGT CCTGGGCGGC TCTGCCCCTT CTATCCAGCA GCAGCACTAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAAGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCTGT GAATTATGCT
    GATGCCGCCC TCTCCTTCAC TGAATGTGGC AATGCCATGG AACGCGACCC TCTGGAAGCA
    AAGTCCCCAT ACACCATGTA CTCTGAGACT GTGGAGCTCC TCAGGTATGC AATGAGGCTG
    AAGAACTTTG CAAGTCCCTT GGCTTCGGAT GGGGACAAAA AGCTAGCAGT ACTATGCTAC
    CGATGTTTAT CACTCCTCTA TTTGAGAATG TTTAAGCTGA AGAAGGACCA TGCTATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC ACAGATACCC
    TCTCCATGGG TAAGCAATGG AAAGAACACT CCATCCCCAG TGTCTCTCAA CAACGTCTCC
    CCCATCAACG CAATGGGGAA CTGTAACAAT GGCCCAGTCA CCATTCCCCA GCGCATTCAC
    CACATGGCTG CCAGCCACGT CAACATCACT AGCAATGTGT TACGGGGCTA TGAACACTGG
    GATATGGCCG ACAAACTGAC AAGAGAAAAC AAAGAATTCT TTGGTGATCT GGACACGCTG
    ATGGGGCCTC TGACCCAGCA CAGCAGCATG ACCAATCTTG TCCGCTACGT TCGCCAAGGA
    CTGTGTTGGC TGCGCATCGA TGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002016.2
    CDS482..4417
    Translation

    Target ORF information:

    RefSeq Version NM_002025.4
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002025.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAGA TGACCCATTC CTGGCCTACT CCTCTCACTT CCATGCATAC TGCTGGACAC
    TCTGAGCAGA GCACCTTTTC CATCCCAGGA CAGGAATCGC AGCATCTGAC CCCAGGATTC
    ACCTTACAAA AGTGGAATGA CCCAACCACC AGAGCTTCTA CAAAGTCAGT GTCTTTCAAA
    TCGATGCTTG AGGATGACCT GAAGCTGAGC AGTGATGAAG ATGACCTTGA GCCTGTGAAG
    ACCTTGACCA CTCAGTGCAC TGCCACTGAG CTCTACCAGG CTGTTGAAAA GGCAAAACCT
    AGGAATAATC CTGTGAACCC ACCCTTGGCC ACTCCCCAGC CCCCACCTGC AGTGCAAGCC
    AGCGGGGGTT CTGGCAGCTC CAGCGAATCG GAGAGCAGCT CTGAGTCGGA TTCAGACACT
    GAAAGTAGCA CCACTGACAG CGAATCTAAT GAGGCACCTC GTGTGGCAAC TCCAGAGCCT
    GAGCCACCCT CAACCAACAA GTGGCAACTG GATAAATGGC TTAACAAAGT GACATCCCAG
    AACAAGTCTT TTATTTGTGG CCAAAATGAA ACACCCATGG AGACTATTTC TCTGCCTCCT
    CCAATCATCC AACCAATGGA AGTCCAGATG AAAGTGAAGA CGAATGCCAG TCAGGTCCCA
    GCTGAACCCA AAGAAAGGCC TCTCCTCAGT CTCATTAGGG AGAAAGCCCG TCCACGGCCC
    ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG AAGCATAAGT TGTCAACAAC TAGTGAGACA
    GTGTCTCAAA GGACAATTGG GAAAAAACAG CCCAAAAAAG TTGAGAAGAA CACCAGCACT
    GACGAGTTTA CCTGGCCCAA ACCAAATATT ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT
    GTGGAGCTTC ATGACCCACC AAGAGGCCGC AACAAAGCCA CTGCCCACAA ACCAGCCCCT
    AGGAAAGAAC CAAGACCTAA CATCCCTTTG GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT
    GGCAAGATTG TGCCAAAGTC TCGGGAATTC ATTGAAACAG ATTCATCTAC ATCTGACTCC
    AACACAGATC AGGAAGAGAC CCTGCAAATC AAAGTCCTGC CTCCGTGCAT TATTTCTGGA
    GGTAATACTG CCAAATCCAA GGAAATCTGT GGTGCCAGCC TGACCCTCAG CACCTTAATG
    AGTAGCAGTG GCAGCAACAA CAACTTATCC ATCAGTAATG AAGAGCCAAC ATTTTCACCT
    ATTCCTGTCA TGCAAACTGA AATCCTGTCC CCTCTGCGAG ATCATGAGAA CCTGAAAAAC
    CTCTGGGTGA AGATTGACCT TGACTTACTC TCTAGAGTAC CTGGCCACAG CTCACTCCAT
    GCAGCACCTG CCAAGCCAGA CCACAAGGAG ACTGCCACAA AACCCAAGCG TCAGACAGCT
    GTCACAGCTG TGGAGAAACC AGCCCCTAAG GGCAAACGTA AGCACAAGCC AATAGAAGTT
    GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC CTGGAGGAGG CCACAACTAT CTGCTTGCTC
    CCTCCTTGCA TCTCACCAGC CCCACCCCAC AAGCCTCCCA ACACTAGAGA AAATAATTCA
    TCCAGGAGAG CAAATAGAAG AAAGGAAGAA AAACTATTTC CTCCTCCACT TTCCCCACTG
    CCAGAGGACC CTCCACGCCG CAGAAATGTC AGTGGCAATA ATGGTCCCTT TGGTCAAGAC
    AAAAACATCG CCATGACTGG ACAAATCACA TCTACCAAAC CTAAGAGAAC TGAAGGCAAA
    TTCTGTGCTA CTTTCAAAGG GATATCGGTA AATGAGGGAG ACACTCCAAA AAAGGCATCC
    TCTGCCACCA TCACTGTCAC CAATACTGCT ATTGCCACTG CTACTGTCAC TGCTACTGCC
    ATTGTCACCA CCACTGTCAC AGCTACTGCC ACCGCCACGG CCACCACCAC AACTACTACC
    ACTACCATTT CCACCATCAC CTCTACCATC ACTACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACGT CCTGGGCGGC TCTGCCCCTT CTATCCAGCA GCAGCACTAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAAGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCTGT GAATTATGCT
    GATGCCGCCC TCTCCTTCAC TGAATGTGGC AATGCCATGG AACGCGACCC TCTGGAAGCA
    AAGTCCCCAT ACACCATGTA CTCTGAGACT GTGGAGCTCC TCAGGTATGC AATGAGGCTG
    AAGAACTTTG CAAGTCCCTT GGCTTCGGAT GGGGACAAAA AGCTAGCAGT ACTATGCTAC
    CGATGTTTAT CACTCCTCTA TTTGAGAATG TTTAAGCTGA AGAAGGACCA TGCTATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC ACAGATACCC
    TCTCCATGGG TAAGCAATGG AAAGAACACT CCATCCCCAG TGTCTCTCAA CAACGTCTCC
    CCCATCAACG CAATGGGGAA CTGTAACAAT GGCCCAGTCA CCATTCCCCA GCGCATTCAC
    CACATGGCTG CCAGCCACGT CAACATCACT AGCAATGTGT TACGGGGCTA TGAACACTGG
    GATATGGCCG ACAAACTGAC AAGAGAAAAC AAAGAATTCT TTGGTGATCT GGACACGCTG
    ATGGGGCCTC TGACCCAGCA CAGCAGCATG ACCAATCTTG TCCGCTACGT TCGCCAAGGA
    CTGTGTTGGC TGCGCATCGA TGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22585
    Clone ID Related Accession (Same CDS sequence) NM_002025.4 , NM_002025.3
    Accession Version NM_002025.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U48436.1 and AC231841.2. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2009 this sequence version replaced NM_002025.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48436.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAGA TGACCCATTC CTGGCCTACT CCTCTCACTT CCATGCATAC TGCTGGACAC
    TCTGAGCAGA GCACCTTTTC CATCCCAGGA CAGGAATCGC AGCATCTGAC CCCAGGATTC
    ACCTTACAAA AGTGGAATGA CCCAACCACC AGAGCTTCTA CAAAGTCAGT GTCTTTCAAA
    TCGATGCTTG AGGATGACCT GAAGCTGAGC AGTGATGAAG ATGACCTTGA GCCTGTGAAG
    ACCTTGACCA CTCAGTGCAC TGCCACTGAG CTCTACCAGG CTGTTGAAAA GGCAAAACCT
    AGGAATAATC CTGTGAACCC ACCCTTGGCC ACTCCCCAGC CCCCACCTGC AGTGCAAGCC
    AGCGGGGGTT CTGGCAGCTC CAGCGAATCG GAGAGCAGCT CTGAGTCGGA TTCAGACACT
    GAAAGTAGCA CCACTGACAG CGAATCTAAT GAGGCACCTC GTGTGGCAAC TCCAGAGCCT
    GAGCCACCCT CAACCAACAA GTGGCAACTG GATAAATGGC TTAACAAAGT GACATCCCAG
    AACAAGTCTT TTATTTGTGG CCAAAATGAA ACACCCATGG AGACTATTTC TCTGCCTCCT
    CCAATCATCC AACCAATGGA AGTCCAGATG AAAGTGAAGA CGAATGCCAG TCAGGTCCCA
    GCTGAACCCA AAGAAAGGCC TCTCCTCAGT CTCATTAGGG AGAAAGCCCG TCCACGGCCC
    ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG AAGCATAAGT TGTCAACAAC TAGTGAGACA
    GTGTCTCAAA GGACAATTGG GAAAAAACAG CCCAAAAAAG TTGAGAAGAA CACCAGCACT
    GACGAGTTTA CCTGGCCCAA ACCAAATATT ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT
    GTGGAGCTTC ATGACCCACC AAGAGGCCGC AACAAAGCCA CTGCCCACAA ACCAGCCCCT
    AGGAAAGAAC CAAGACCTAA CATCCCTTTG GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT
    GGCAAGATTG TGCCAAAGTC TCGGGAATTC ATTGAAACAG ATTCATCTAC ATCTGACTCC
    AACACAGATC AGGAAGAGAC CCTGCAAATC AAAGTCCTGC CTCCGTGCAT TATTTCTGGA
    GGTAATACTG CCAAATCCAA GGAAATCTGT GGTGCCAGCC TGACCCTCAG CACCTTAATG
    AGTAGCAGTG GCAGCAACAA CAACTTATCC ATCAGTAATG AAGAGCCAAC ATTTTCACCT
    ATTCCTGTCA TGCAAACTGA AATCCTGTCC CCTCTGCGAG ATCATGAGAA CCTGAAAAAC
    CTCTGGGTGA AGATTGACCT TGACTTACTC TCTAGAGTAC CTGGCCACAG CTCACTCCAT
    GCAGCACCTG CCAAGCCAGA CCACAAGGAG ACTGCCACAA AACCCAAGCG TCAGACAGCT
    GTCACAGCTG TGGAGAAACC AGCCCCTAAG GGCAAACGTA AGCACAAGCC AATAGAAGTT
    GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC CTGGAGGAGG CCACAACTAT CTGCTTGCTC
    CCTCCTTGCA TCTCACCAGC CCCACCCCAC AAGCCTCCCA ACACTAGAGA AAATAATTCA
    TCCAGGAGAG CAAATAGAAG AAAGGAAGAA AAACTATTTC CTCCTCCACT TTCCCCACTG
    CCAGAGGACC CTCCACGCCG CAGAAATGTC AGTGGCAATA ATGGTCCCTT TGGTCAAGAC
    AAAAACATCG CCATGACTGG ACAAATCACA TCTACCAAAC CTAAGAGAAC TGAAGGCAAA
    TTCTGTGCTA CTTTCAAAGG GATATCGGTA AATGAGGGAG ACACTCCAAA AAAGGCATCC
    TCTGCCACCA TCACTGTCAC CAATACTGCT ATTGCCACTG CTACTGTCAC TGCTACTGCC
    ATTGTCACCA CCACTGTCAC AGCTACTGCC ACCGCCACGG CCACCACCAC AACTACTACC
    ACTACCATTT CCACCATCAC CTCTACCATC ACTACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACGT CCTGGGCGGC TCTGCCCCTT CTATCCAGCA GCAGCACTAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAAGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCTGT GAATTATGCT
    GATGCCGCCC TCTCCTTCAC TGAATGTGGC AATGCCATGG AACGCGACCC TCTGGAAGCA
    AAGTCCCCAT ACACCATGTA CTCTGAGACT GTGGAGCTCC TCAGGTATGC AATGAGGCTG
    AAGAACTTTG CAAGTCCCTT GGCTTCGGAT GGGGACAAAA AGCTAGCAGT ACTATGCTAC
    CGATGTTTAT CACTCCTCTA TTTGAGAATG TTTAAGCTGA AGAAGGACCA TGCTATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC ACAGATACCC
    TCTCCATGGG TAAGCAATGG AAAGAACACT CCATCCCCAG TGTCTCTCAA CAACGTCTCC
    CCCATCAACG CAATGGGGAA CTGTAACAAT GGCCCAGTCA CCATTCCCCA GCGCATTCAC
    CACATGGCTG CCAGCCACGT CAACATCACT AGCAATGTGT TACGGGGCTA TGAACACTGG
    GATATGGCCG ACAAACTGAC AAGAGAAAAC AAAGAATTCT TTGGTGATCT GGACACGCTG
    ATGGGGCCTC TGACCCAGCA CAGCAGCATG ACCAATCTTG TCCGCTACGT TCGCCAAGGA
    CTGTGTTGGC TGCGCATCGA TGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002016.2
    CDS480..4415
    Translation

    Target ORF information:

    RefSeq Version NM_002025.3
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAGA TGACCCATTC CTGGCCTACT CCTCTCACTT CCATGCATAC TGCTGGACAC
    TCTGAGCAGA GCACCTTTTC CATCCCAGGA CAGGAATCGC AGCATCTGAC CCCAGGATTC
    ACCTTACAAA AGTGGAATGA CCCAACCACC AGAGCTTCTA CAAAGTCAGT GTCTTTCAAA
    TCGATGCTTG AGGATGACCT GAAGCTGAGC AGTGATGAAG ATGACCTTGA GCCTGTGAAG
    ACCTTGACCA CTCAGTGCAC TGCCACTGAG CTCTACCAGG CTGTTGAAAA GGCAAAACCT
    AGGAATAATC CTGTGAACCC ACCCTTGGCC ACTCCCCAGC CCCCACCTGC AGTGCAAGCC
    AGCGGGGGTT CTGGCAGCTC CAGCGAATCG GAGAGCAGCT CTGAGTCGGA TTCAGACACT
    GAAAGTAGCA CCACTGACAG CGAATCTAAT GAGGCACCTC GTGTGGCAAC TCCAGAGCCT
    GAGCCACCCT CAACCAACAA GTGGCAACTG GATAAATGGC TTAACAAAGT GACATCCCAG
    AACAAGTCTT TTATTTGTGG CCAAAATGAA ACACCCATGG AGACTATTTC TCTGCCTCCT
    CCAATCATCC AACCAATGGA AGTCCAGATG AAAGTGAAGA CGAATGCCAG TCAGGTCCCA
    GCTGAACCCA AAGAAAGGCC TCTCCTCAGT CTCATTAGGG AGAAAGCCCG TCCACGGCCC
    ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG AAGCATAAGT TGTCAACAAC TAGTGAGACA
    GTGTCTCAAA GGACAATTGG GAAAAAACAG CCCAAAAAAG TTGAGAAGAA CACCAGCACT
    GACGAGTTTA CCTGGCCCAA ACCAAATATT ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT
    GTGGAGCTTC ATGACCCACC AAGAGGCCGC AACAAAGCCA CTGCCCACAA ACCAGCCCCT
    AGGAAAGAAC CAAGACCTAA CATCCCTTTG GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT
    GGCAAGATTG TGCCAAAGTC TCGGGAATTC ATTGAAACAG ATTCATCTAC ATCTGACTCC
    AACACAGATC AGGAAGAGAC CCTGCAAATC AAAGTCCTGC CTCCGTGCAT TATTTCTGGA
    GGTAATACTG CCAAATCCAA GGAAATCTGT GGTGCCAGCC TGACCCTCAG CACCTTAATG
    AGTAGCAGTG GCAGCAACAA CAACTTATCC ATCAGTAATG AAGAGCCAAC ATTTTCACCT
    ATTCCTGTCA TGCAAACTGA AATCCTGTCC CCTCTGCGAG ATCATGAGAA CCTGAAAAAC
    CTCTGGGTGA AGATTGACCT TGACTTACTC TCTAGAGTAC CTGGCCACAG CTCACTCCAT
    GCAGCACCTG CCAAGCCAGA CCACAAGGAG ACTGCCACAA AACCCAAGCG TCAGACAGCT
    GTCACAGCTG TGGAGAAACC AGCCCCTAAG GGCAAACGTA AGCACAAGCC AATAGAAGTT
    GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC CTGGAGGAGG CCACAACTAT CTGCTTGCTC
    CCTCCTTGCA TCTCACCAGC CCCACCCCAC AAGCCTCCCA ACACTAGAGA AAATAATTCA
    TCCAGGAGAG CAAATAGAAG AAAGGAAGAA AAACTATTTC CTCCTCCACT TTCCCCACTG
    CCAGAGGACC CTCCACGCCG CAGAAATGTC AGTGGCAATA ATGGTCCCTT TGGTCAAGAC
    AAAAACATCG CCATGACTGG ACAAATCACA TCTACCAAAC CTAAGAGAAC TGAAGGCAAA
    TTCTGTGCTA CTTTCAAAGG GATATCGGTA AATGAGGGAG ACACTCCAAA AAAGGCATCC
    TCTGCCACCA TCACTGTCAC CAATACTGCT ATTGCCACTG CTACTGTCAC TGCTACTGCC
    ATTGTCACCA CCACTGTCAC AGCTACTGCC ACCGCCACGG CCACCACCAC AACTACTACC
    ACTACCATTT CCACCATCAC CTCTACCATC ACTACTGGCC TCATGGATAG CAGTCACCTG
    GAGATGACGT CCTGGGCGGC TCTGCCCCTT CTATCCAGCA GCAGCACTAA TGTCCGGAGA
    CCCAAGCTCA CTTTTGATGA CTCGGTTCAC AATGCTGATT ATTACATGCA AGAAGCTAAG
    AAGCTGAAGC ACAAAGCTGA TGCACTGTTC GAGAAATTTG GCAAAGCTGT GAATTATGCT
    GATGCCGCCC TCTCCTTCAC TGAATGTGGC AATGCCATGG AACGCGACCC TCTGGAAGCA
    AAGTCCCCAT ACACCATGTA CTCTGAGACT GTGGAGCTCC TCAGGTATGC AATGAGGCTG
    AAGAACTTTG CAAGTCCCTT GGCTTCGGAT GGGGACAAAA AGCTAGCAGT ACTATGCTAC
    CGATGTTTAT CACTCCTCTA TTTGAGAATG TTTAAGCTGA AGAAGGACCA TGCTATGAAG
    TACTCCAGAT CACTGATGGA ATATTTTAAG CAAAATGCTT CAAAAGTCGC ACAGATACCC
    TCTCCATGGG TAAGCAATGG AAAGAACACT CCATCCCCAG TGTCTCTCAA CAACGTCTCC
    CCCATCAACG CAATGGGGAA CTGTAACAAT GGCCCAGTCA CCATTCCCCA GCGCATTCAC
    CACATGGCTG CCAGCCACGT CAACATCACT AGCAATGTGT TACGGGGCTA TGAACACTGG
    GATATGGCCG ACAAACTGAC AAGAGAAAAC AAAGAATTCT TTGGTGATCT GGACACGCTG
    ATGGGGCCTC TGACCCAGCA CAGCAGCATG ACCAATCTTG TCCGCTACGT TCGCCAAGGA
    CTGTGTTGGC TGCGCATCGA TGCCCACTTG TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08710
    Clone ID Related Accession (Same CDS sequence) NM_001170628.1
    Accession Version NM_001170628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-03
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301927.1, AC002368.1 and AC231841.2. Transcript Variant: This variant (6) has multiple differences, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (6) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAAGGAGGCA CCAAGCATTT CCGTCCTTCT TCAAGATGAA GGTAAGCCTT 
    CCCAGTGATC CAAGCTGTGT TGAAGAAATC TTGCGGGAAT CGCAGCATCT GACCCCAGGA
    TTCACCTTAC AAAAGTGGAA TGACCCAACC ACCAGAGCTT CTACAAAGTC AGTGTCTTTC
    AAATCGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164099.1
    CDS91..2949
    Translation

    Target ORF information:

    RefSeq Version NM_001170628.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAAGGAGGCA CCAAGCATTT CCGTCCTTCT TCAAGATGAA GGTAAGCCTT 
    CCCAGTGATC CAAGCTGTGT TGAAGAAATC TTGCGGGAAT CGCAGCATCT GACCCCAGGA
    TTCACCTTAC AAAAGTGGAA TGACCCAACC ACCAGAGCTT CTACAAAGTC AGTGTCTTTC
    AAATCGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10427
    Clone ID Related Accession (Same CDS sequence) NM_001169125.1 , NM_001169125.2
    Accession Version NM_001169125.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U48436.1, BC143740.1 and AC231841.2. Transcript Variant: This variant (5) has multiple differences, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143740.1, AB102644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162596.1
    CDS480..4298
    Translation

    Target ORF information:

    RefSeq Version NM_001169125.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10427
    Clone ID Related Accession (Same CDS sequence) NM_001169125.1 , NM_001169125.2
    Accession Version NM_001169125.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002368.1, U48436.1, BC143740.1 and AC231841.2. On May 31, 2019 this sequence version replaced NM_001169125.1. Transcript Variant: This variant (5) has multiple differences, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143740.1, AB102644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162596.1
    CDS482..4300
    Translation

    Target ORF information:

    RefSeq Version NM_001169125.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169125.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGATGC TTGAGGATGA CCTGAAGCTG AGCAGTGATG AAGATGACCT TGAGCCTGTG
    AAGACCTTGA CCACTCAGTG CACTGCCACT GAGCTCTACC AGGCTGTTGA AAAGGCAAAA
    CCTAGGAATA ATCCTGTGAA CCCACCCTTG GCCACTCCCC AGCCCCCACC TGCAGTGCAA
    GCCAGCGGGG GTTCTGGCAG CTCCAGCGAA TCGGAGAGCA GCTCTGAGTC GGATTCAGAC
    ACTGAAAGTA GCACCACTGA CAGCGAATCT AATGAGGCAC CTCGTGTGGC AACTCCAGAG
    CCTGAGCCAC CCTCAACCAA CAAGTGGCAA CTGGATAAAT GGCTTAACAA AGTGACATCC
    CAGAACAAGT CTTTTATTTG TGGCCAAAAT GAAACACCCA TGGAGACTAT TTCTCTGCCT
    CCTCCAATCA TCCAACCAAT GGAAGTCCAG ATGAAAGTGA AGACGAATGC CAGTCAGGTC
    CCAGCTGAAC CCAAAGAAAG GCCTCTCCTC AGTCTCATTA GGGAGAAAGC CCGTCCACGG
    CCCACTCAGA AAATTCCAGA AACAAAGGCT TTGAAGCATA AGTTGTCAAC AACTAGTGAG
    ACAGTGTCTC AAAGGACAAT TGGGAAAAAA CAGCCCAAAA AAGTTGAGAA GAACACCAGC
    ACTGACGAGT TTACCTGGCC CAAACCAAAT ATTACCAGCA GCACTCCCAA AGAAAAAGAA
    AGTGTGGAGC TTCATGACCC ACCAAGAGGC CGCAACAAAG CCACTGCCCA CAAACCAGCC
    CCTAGGAAAG AACCAAGACC TAACATCCCT TTGGCTCCCG AGAAGAAGAA GTACAGAGGG
    CCTGGCAAGA TTGTGCCAAA GTCTCGGGAA TTCATTGAAA CAGATTCATC TACATCTGAC
    TCCAACACAG ATCAGGAAGA GACCCTGCAA ATCAAAGTCC TGCCTCCGTG CATTATTTCT
    GGAGGTAATA CTGCCAAATC CAAGGAAATC TGTGGTGCCA GCCTGACCCT CAGCACCTTA
    ATGAGTAGCA GTGGCAGCAA CAACAACTTA TCCATCAGTA ATGAAGAGCC AACATTTTCA
    CCTATTCCTG TCATGCAAAC TGAAATCCTG TCCCCTCTGC GAGATCATGA GAACCTGAAA
    AACCTCTGGG TGAAGATTGA CCTTGACTTA CTCTCTAGAG TACCTGGCCA CAGCTCACTC
    CATGCAGCAC CTGCCAAGCC AGACCACAAG GAGACTGCCA CAAAACCCAA GCGTCAGACA
    GCTGTCACAG CTGTGGAGAA ACCAGCCCCT AAGGGCAAAC GTAAGCACAA GCCAATAGAA
    GTTGCAGAGA AGATCCCTGA GAAGAAGCAG CGCCTGGAGG AGGCCACAAC TATCTGCTTG
    CTCCCTCCTT GCATCTCACC AGCCCCACCC CACAAGCCTC CCAACACTAG AGAAAATAAT
    TCATCCAGGA GAGCAAATAG AAGAAAGGAA GAAAAACTAT TTCCTCCTCC ACTTTCCCCA
    CTGCCAGAGG ACCCTCCACG CCGCAGAAAT GTCAGTGGCA ATAATGGTCC CTTTGGTCAA
    GACAAAAACA TCGCCATGAC TGGACAAATC ACATCTACCA AACCTAAGAG AACTGAAGGC
    AAATTCTGTG CTACTTTCAA AGGGATATCG GTAAATGAGG GAGACACTCC AAAAAAGGCA
    TCCTCTGCCA CCATCACTGT CACCAATACT GCTATTGCCA CTGCTACTGT CACTGCTACT
    GCCATTGTCA CCACCACTGT CACAGCTACT GCCACCGCCA CGGCCACCAC CACAACTACT
    ACCACTACCA TTTCCACCAT CACCTCTACC ATCACTACTG GCCTCATGGA TAGCAGTCAC
    CTGGAGATGA CGTCCTGGGC GGCTCTGCCC CTTCTATCCA GCAGCAGCAC TAATGTCCGG
    AGACCCAAGC TCACTTTTGA TGACTCGGTT CACAATGCTG ATTATTACAT GCAAGAAGCT
    AAGAAGCTGA AGCACAAAGC TGATGCACTG TTCGAGAAAT TTGGCAAAGC TGTGAATTAT
    GCTGATGCCG CCCTCTCCTT CACTGAATGT GGCAATGCCA TGGAACGCGA CCCTCTGGAA
    GCAAAGTCCC CATACACCAT GTACTCTGAG ACTGTGGAGC TCCTCAGGTA TGCAATGAGG
    CTGAAGAACT TTGCAAGTCC CTTGGCTTCG GATGGGGACA AAAAGCTAGC AGTACTATGC
    TACCGATGTT TATCACTCCT CTATTTGAGA ATGTTTAAGC TGAAGAAGGA CCATGCTATG
    AAGTACTCCA GATCACTGAT GGAATATTTT AAGCAAAATG CTTCAAAAGT CGCACAGATA
    CCCTCTCCAT GGGTAAGCAA TGGAAAGAAC ACTCCATCCC CAGTGTCTCT CAACAACGTC
    TCCCCCATCA ACGCAATGGG GAACTGTAAC AATGGCCCAG TCACCATTCC CCAGCGCATT
    CACCACATGG CTGCCAGCCA CGTCAACATC ACTAGCAATG TGTTACGGGG CTATGAACAC
    TGGGATATGG CCGACAAACT GACAAGAGAA AACAAAGAAT TCTTTGGTGA TCTGGACACG
    CTGATGGGGC CTCTGACCCA GCACAGCAGC ATGACCAATC TTGTCCGCTA CGTTCGCCAA
    GGACTGTGTT GGCTGCGCAT CGATGCCCAC TTGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11095
    Clone ID Related Accession (Same CDS sequence) NM_001169124.1 , NM_001169124.2
    Accession Version NM_001169124.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U48436.1, BC143744.1 and AC231841.2. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAAT CGCAGCATCT GACCCCAGGA TTCACCTTAC AAAAGTGGAA TGACCCAACC
    ACCAGAGCTT CTACAAAGAT GCTTGAGGAT GACCTGAAGC TGAGCAGTGA TGAAGATGAC
    CTTGAGCCTG TGAAGACCTT GACCACTCAG TGCACTGCCA CTGAGCTCTA CCAGGCTGTT
    GAAAAGGCAA AACCTAGGAA TAATCCTGTG AACCCACCCT TGGCCACTCC CCAGCCCCCA
    CCTGCAGTGC AAGCCAGCGG GGGTTCTGGC AGCTCCAGCG AATCGGAGAG CAGCTCTGAG
    TCGGATTCAG ACACTGAAAG TAGCACCACT GACAGCGAAT CTAATGAGGC ACCTCGTGTG
    GCAACTCCAG AGCCTGAGCC ACCCTCAACC AACAAGTGGC AACTGGATAA ATGGCTTAAC
    AAAGTGACAT CCCAGAACAA GTCTTTTATT TGTGGCCAAA ATGAAACACC CATGGAGACT
    ATTTCTCTGC CTCCTCCAAT CATCCAACCA ATGGAAGTCC AGATGAAAGT GAAGACGAAT
    GCCAGTCAGG TCCCAGCTGA ACCCAAAGAA AGGCCTCTCC TCAGTCTCAT TAGGGAGAAA
    GCCCGTCCAC GGCCCACTCA GAAAATTCCA GAAACAAAGG CTTTGAAGCA TAAGTTGTCA
    ACAACTAGTG AGACAGTGTC TCAAAGGACA ATTGGGAAAA AACAGCCCAA AAAAGTTGAG
    AAGAACACCA GCACTGACGA GTTTACCTGG CCCAAACCAA ATATTACCAG CAGCACTCCC
    AAAGAAAAAG AAAGTGTGGA GCTTCATGAC CCACCAAGAG GCCGCAACAA AGCCACTGCC
    CACAAACCAG CCCCTAGGAA AGAACCAAGA CCTAACATCC CTTTGGCTCC CGAGAAGAAG
    AAGTACAGAG GGCCTGGCAA GATTGTGCCA AAGTCTCGGG AATTCATTGA AACAGATTCA
    TCTACATCTG ACTCCAACAC AGATCAGGAA GAGACCCTGC AAATCAAAGT CCTGCCTCCG
    TGCATTATTT CTGGAGGTAA TACTGCCAAA TCCAAGGAAA TCTGTGGTGC CAGCCTGACC
    CTCAGCACCT TAATGAGTAG CAGTGGCAGC AACAACAACT TATCCATCAG TAATGAAGAG
    CCAACATTTT CACCTATTCC TGTCATGCAA ACTGAAATCC TGTCCCCTCT GCGAGATCAT
    GAGAACCTGA AAAACCTCTG GGTGAAGATT GACCTTGACT TACTCTCTAG AGTACCTGGC
    CACAGCTCAC TCCATGCAGC ACCTGCCAAG CCAGACCACA AGGAGACTGC CACAAAACCC
    AAGCGTCAGA CAGCTGTCAC AGCTGTGGAG AAACCAGCCC CTAAGGGCAA ACGTAAGCAC
    AAGCCAATAG AAGTTGCAGA GAAGATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCCACA
    ACTATCTGCT TGCTCCCTCC TTGCATCTCA CCAGCCCCAC CCCACAAGCC TCCCAACACT
    AGAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAGG AAGAAAAACT ATTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGCCGCAGAA ATGTCAGTGG CAATAATGGT
    CCCTTTGGTC AAGACAAAAA CATCGCCATG ACTGGACAAA TCACATCTAC CAAACCTAAG
    AGAACTGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAT CGGTAAATGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162595.1
    CDS480..4310
    Translation

    Target ORF information:

    RefSeq Version NM_001169124.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAAT CGCAGCATCT GACCCCAGGA TTCACCTTAC AAAAGTGGAA TGACCCAACC
    ACCAGAGCTT CTACAAAGAT GCTTGAGGAT GACCTGAAGC TGAGCAGTGA TGAAGATGAC
    CTTGAGCCTG TGAAGACCTT GACCACTCAG TGCACTGCCA CTGAGCTCTA CCAGGCTGTT
    GAAAAGGCAA AACCTAGGAA TAATCCTGTG AACCCACCCT TGGCCACTCC CCAGCCCCCA
    CCTGCAGTGC AAGCCAGCGG GGGTTCTGGC AGCTCCAGCG AATCGGAGAG CAGCTCTGAG
    TCGGATTCAG ACACTGAAAG TAGCACCACT GACAGCGAAT CTAATGAGGC ACCTCGTGTG
    GCAACTCCAG AGCCTGAGCC ACCCTCAACC AACAAGTGGC AACTGGATAA ATGGCTTAAC
    AAAGTGACAT CCCAGAACAA GTCTTTTATT TGTGGCCAAA ATGAAACACC CATGGAGACT
    ATTTCTCTGC CTCCTCCAAT CATCCAACCA ATGGAAGTCC AGATGAAAGT GAAGACGAAT
    GCCAGTCAGG TCCCAGCTGA ACCCAAAGAA AGGCCTCTCC TCAGTCTCAT TAGGGAGAAA
    GCCCGTCCAC GGCCCACTCA GAAAATTCCA GAAACAAAGG CTTTGAAGCA TAAGTTGTCA
    ACAACTAGTG AGACAGTGTC TCAAAGGACA ATTGGGAAAA AACAGCCCAA AAAAGTTGAG
    AAGAACACCA GCACTGACGA GTTTACCTGG CCCAAACCAA ATATTACCAG CAGCACTCCC
    AAAGAAAAAG AAAGTGTGGA GCTTCATGAC CCACCAAGAG GCCGCAACAA AGCCACTGCC
    CACAAACCAG CCCCTAGGAA AGAACCAAGA CCTAACATCC CTTTGGCTCC CGAGAAGAAG
    AAGTACAGAG GGCCTGGCAA GATTGTGCCA AAGTCTCGGG AATTCATTGA AACAGATTCA
    TCTACATCTG ACTCCAACAC AGATCAGGAA GAGACCCTGC AAATCAAAGT CCTGCCTCCG
    TGCATTATTT CTGGAGGTAA TACTGCCAAA TCCAAGGAAA TCTGTGGTGC CAGCCTGACC
    CTCAGCACCT TAATGAGTAG CAGTGGCAGC AACAACAACT TATCCATCAG TAATGAAGAG
    CCAACATTTT CACCTATTCC TGTCATGCAA ACTGAAATCC TGTCCCCTCT GCGAGATCAT
    GAGAACCTGA AAAACCTCTG GGTGAAGATT GACCTTGACT TACTCTCTAG AGTACCTGGC
    CACAGCTCAC TCCATGCAGC ACCTGCCAAG CCAGACCACA AGGAGACTGC CACAAAACCC
    AAGCGTCAGA CAGCTGTCAC AGCTGTGGAG AAACCAGCCC CTAAGGGCAA ACGTAAGCAC
    AAGCCAATAG AAGTTGCAGA GAAGATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCCACA
    ACTATCTGCT TGCTCCCTCC TTGCATCTCA CCAGCCCCAC CCCACAAGCC TCCCAACACT
    AGAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAGG AAGAAAAACT ATTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGCCGCAGAA ATGTCAGTGG CAATAATGGT
    CCCTTTGGTC AAGACAAAAA CATCGCCATG ACTGGACAAA TCACATCTAC CAAACCTAAG
    AGAACTGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAT CGGTAAATGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11095
    Clone ID Related Accession (Same CDS sequence) NM_001169124.1 , NM_001169124.2
    Accession Version NM_001169124.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002368.1, U48436.1, BC143744.1 and AC231841.2. On May 31, 2019 this sequence version replaced NM_001169124.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAAT CGCAGCATCT GACCCCAGGA TTCACCTTAC AAAAGTGGAA TGACCCAACC
    ACCAGAGCTT CTACAAAGAT GCTTGAGGAT GACCTGAAGC TGAGCAGTGA TGAAGATGAC
    CTTGAGCCTG TGAAGACCTT GACCACTCAG TGCACTGCCA CTGAGCTCTA CCAGGCTGTT
    GAAAAGGCAA AACCTAGGAA TAATCCTGTG AACCCACCCT TGGCCACTCC CCAGCCCCCA
    CCTGCAGTGC AAGCCAGCGG GGGTTCTGGC AGCTCCAGCG AATCGGAGAG CAGCTCTGAG
    TCGGATTCAG ACACTGAAAG TAGCACCACT GACAGCGAAT CTAATGAGGC ACCTCGTGTG
    GCAACTCCAG AGCCTGAGCC ACCCTCAACC AACAAGTGGC AACTGGATAA ATGGCTTAAC
    AAAGTGACAT CCCAGAACAA GTCTTTTATT TGTGGCCAAA ATGAAACACC CATGGAGACT
    ATTTCTCTGC CTCCTCCAAT CATCCAACCA ATGGAAGTCC AGATGAAAGT GAAGACGAAT
    GCCAGTCAGG TCCCAGCTGA ACCCAAAGAA AGGCCTCTCC TCAGTCTCAT TAGGGAGAAA
    GCCCGTCCAC GGCCCACTCA GAAAATTCCA GAAACAAAGG CTTTGAAGCA TAAGTTGTCA
    ACAACTAGTG AGACAGTGTC TCAAAGGACA ATTGGGAAAA AACAGCCCAA AAAAGTTGAG
    AAGAACACCA GCACTGACGA GTTTACCTGG CCCAAACCAA ATATTACCAG CAGCACTCCC
    AAAGAAAAAG AAAGTGTGGA GCTTCATGAC CCACCAAGAG GCCGCAACAA AGCCACTGCC
    CACAAACCAG CCCCTAGGAA AGAACCAAGA CCTAACATCC CTTTGGCTCC CGAGAAGAAG
    AAGTACAGAG GGCCTGGCAA GATTGTGCCA AAGTCTCGGG AATTCATTGA AACAGATTCA
    TCTACATCTG ACTCCAACAC AGATCAGGAA GAGACCCTGC AAATCAAAGT CCTGCCTCCG
    TGCATTATTT CTGGAGGTAA TACTGCCAAA TCCAAGGAAA TCTGTGGTGC CAGCCTGACC
    CTCAGCACCT TAATGAGTAG CAGTGGCAGC AACAACAACT TATCCATCAG TAATGAAGAG
    CCAACATTTT CACCTATTCC TGTCATGCAA ACTGAAATCC TGTCCCCTCT GCGAGATCAT
    GAGAACCTGA AAAACCTCTG GGTGAAGATT GACCTTGACT TACTCTCTAG AGTACCTGGC
    CACAGCTCAC TCCATGCAGC ACCTGCCAAG CCAGACCACA AGGAGACTGC CACAAAACCC
    AAGCGTCAGA CAGCTGTCAC AGCTGTGGAG AAACCAGCCC CTAAGGGCAA ACGTAAGCAC
    AAGCCAATAG AAGTTGCAGA GAAGATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCCACA
    ACTATCTGCT TGCTCCCTCC TTGCATCTCA CCAGCCCCAC CCCACAAGCC TCCCAACACT
    AGAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAGG AAGAAAAACT ATTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGCCGCAGAA ATGTCAGTGG CAATAATGGT
    CCCTTTGGTC AAGACAAAAA CATCGCCATG ACTGGACAAA TCACATCTAC CAAACCTAAG
    AGAACTGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAT CGGTAAATGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162595.1
    CDS482..4312
    Translation

    Target ORF information:

    RefSeq Version NM_001169124.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169124.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGGT AGCTGAATAT
    ACAAACAAAG GTGATGCACT TGCCAACCGA GTCCAGAACA CGCTTGGAAA CTATGATGAA
    ATGAAGAATT TGCTAACTAA CCATTCTAAT CAGAATCACC TAGTGGGAAT TCCAAAGAAT
    TCTGTGCCCC AGAATCCCAA CAACAAAAAT GAACCAAGCT TTTTTCCAGA ACAAAAGAAC
    AGAATAATTC CACCTCACCA GGATAATACC CATCCTTCAG CACCAATGCC TCCACCTTCT
    GTTGTGATAC TGAATTCAAC TCTAATACAC AGCAACAGAA AATCAAAACC TGAGTGGTCA
    CGTGATAGTC ATAACCCTAG CACTGTACTG GCAAGCCAGG CCAGTGGTCA GCCAAACAAG
    ATGCAGACTT TGACACAGGA CCAGTCTCAA GCCAAACTGG AAGACTTCTT TGTCTACCCA
    GCTGAACAGC CCCAGATTGG AGAAGTTGAA GAGTCAAACC CATCTGCAAA GGAAGACAGT
    AACCCTAATT CTAGTGGAGA AGATGCTTTC AAAGAAATCT TTCAATCCAA TTCACCGGAA
    GAATCTGAAT TCGCCGTGCA AGCGCCTGGG TCTCCCCTAG TGGCTTCCTC TTTATTAGCT
    CCTAGCAGTG GCCTTTCAGT TCAAAACTTC CCACCAGGGC TTTACTGCAA AACAAGCATG
    GGGCAGCAAA AGCCAACTGC ATACGTCAGA CCCATGGATG GCCAGGACCA GGCACCGGAC
    ATCTCACCAA CACTGAAACC TTCAATTGAA TTTGAGAACA GCTTTGGGAA TCTGTCATTT
    GGAACACTCT TGGATGGAAA ACCCAGTGCA GCCAGTTCAA AGACTAAACT GCCAAAGTTC
    ACCATCCTCC AAACAAGTGA AGTAAGCCTT CCCAGTGATC CAAGCTGTGT TGAAGAAATC
    TTGCGGGAAT CGCAGCATCT GACCCCAGGA TTCACCTTAC AAAAGTGGAA TGACCCAACC
    ACCAGAGCTT CTACAAAGAT GCTTGAGGAT GACCTGAAGC TGAGCAGTGA TGAAGATGAC
    CTTGAGCCTG TGAAGACCTT GACCACTCAG TGCACTGCCA CTGAGCTCTA CCAGGCTGTT
    GAAAAGGCAA AACCTAGGAA TAATCCTGTG AACCCACCCT TGGCCACTCC CCAGCCCCCA
    CCTGCAGTGC AAGCCAGCGG GGGTTCTGGC AGCTCCAGCG AATCGGAGAG CAGCTCTGAG
    TCGGATTCAG ACACTGAAAG TAGCACCACT GACAGCGAAT CTAATGAGGC ACCTCGTGTG
    GCAACTCCAG AGCCTGAGCC ACCCTCAACC AACAAGTGGC AACTGGATAA ATGGCTTAAC
    AAAGTGACAT CCCAGAACAA GTCTTTTATT TGTGGCCAAA ATGAAACACC CATGGAGACT
    ATTTCTCTGC CTCCTCCAAT CATCCAACCA ATGGAAGTCC AGATGAAAGT GAAGACGAAT
    GCCAGTCAGG TCCCAGCTGA ACCCAAAGAA AGGCCTCTCC TCAGTCTCAT TAGGGAGAAA
    GCCCGTCCAC GGCCCACTCA GAAAATTCCA GAAACAAAGG CTTTGAAGCA TAAGTTGTCA
    ACAACTAGTG AGACAGTGTC TCAAAGGACA ATTGGGAAAA AACAGCCCAA AAAAGTTGAG
    AAGAACACCA GCACTGACGA GTTTACCTGG CCCAAACCAA ATATTACCAG CAGCACTCCC
    AAAGAAAAAG AAAGTGTGGA GCTTCATGAC CCACCAAGAG GCCGCAACAA AGCCACTGCC
    CACAAACCAG CCCCTAGGAA AGAACCAAGA CCTAACATCC CTTTGGCTCC CGAGAAGAAG
    AAGTACAGAG GGCCTGGCAA GATTGTGCCA AAGTCTCGGG AATTCATTGA AACAGATTCA
    TCTACATCTG ACTCCAACAC AGATCAGGAA GAGACCCTGC AAATCAAAGT CCTGCCTCCG
    TGCATTATTT CTGGAGGTAA TACTGCCAAA TCCAAGGAAA TCTGTGGTGC CAGCCTGACC
    CTCAGCACCT TAATGAGTAG CAGTGGCAGC AACAACAACT TATCCATCAG TAATGAAGAG
    CCAACATTTT CACCTATTCC TGTCATGCAA ACTGAAATCC TGTCCCCTCT GCGAGATCAT
    GAGAACCTGA AAAACCTCTG GGTGAAGATT GACCTTGACT TACTCTCTAG AGTACCTGGC
    CACAGCTCAC TCCATGCAGC ACCTGCCAAG CCAGACCACA AGGAGACTGC CACAAAACCC
    AAGCGTCAGA CAGCTGTCAC AGCTGTGGAG AAACCAGCCC CTAAGGGCAA ACGTAAGCAC
    AAGCCAATAG AAGTTGCAGA GAAGATCCCT GAGAAGAAGC AGCGCCTGGA GGAGGCCACA
    ACTATCTGCT TGCTCCCTCC TTGCATCTCA CCAGCCCCAC CCCACAAGCC TCCCAACACT
    AGAGAAAATA ATTCATCCAG GAGAGCAAAT AGAAGAAAGG AAGAAAAACT ATTTCCTCCT
    CCACTTTCCC CACTGCCAGA GGACCCTCCA CGCCGCAGAA ATGTCAGTGG CAATAATGGT
    CCCTTTGGTC AAGACAAAAA CATCGCCATG ACTGGACAAA TCACATCTAC CAAACCTAAG
    AGAACTGAAG GCAAATTCTG TGCTACTTTC AAAGGGATAT CGGTAAATGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12312
    Clone ID Related Accession (Same CDS sequence) NM_001169123.2 , NM_001169123.1
    Accession Version NM_001169123.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002368.1, U48436.1, BC132683.1 and AC231841.2. On Jun 1, 2019 this sequence version replaced NM_001169123.1. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAGATG
    ACCCATTCCT GGCCTACTCC TCTCACTTCC ATGCATACTG CTGGACACTC TGAGCAGAGC
    ACCTTTTCCA TCCCAGGACA GGAATCGCAG CATCTGACCC CAGGATTCAC CTTACAAAAG
    TGGAATGACC CAACCACCAG AGCTTCTACA AAGATGCTTG AGGATGACCT GAAGCTGAGC
    AGTGATGAAG ATGACCTTGA GCCTGTGAAG ACCTTGACCA CTCAGTGCAC TGCCACTGAG
    CTCTACCAGG CTGTTGAAAA GGCAAAACCT AGGAATAATC CTGTGAACCC ACCCTTGGCC
    ACTCCCCAGC CCCCACCTGC AGTGCAAGCC AGCGGGGGTT CTGGCAGCTC CAGCGAATCG
    GAGAGCAGCT CTGAGTCGGA TTCAGACACT GAAAGTAGCA CCACTGACAG CGAATCTAAT
    GAGGCACCTC GTGTGGCAAC TCCAGAGCCT GAGCCACCCT CAACCAACAA GTGGCAACTG
    GATAAATGGC TTAACAAAGT GACATCCCAG AACAAGTCTT TTATTTGTGG CCAAAATGAA
    ACACCCATGG AGACTATTTC TCTGCCTCCT CCAATCATCC AACCAATGGA AGTCCAGATG
    AAAGTGAAGA CGAATGCCAG TCAGGTCCCA GCTGAACCCA AAGAAAGGCC TCTCCTCAGT
    CTCATTAGGG AGAAAGCCCG TCCACGGCCC ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG
    AAGCATAAGT TGTCAACAAC TAGTGAGACA GTGTCTCAAA GGACAATTGG GAAAAAACAG
    CCCAAAAAAG TTGAGAAGAA CACCAGCACT GACGAGTTTA CCTGGCCCAA ACCAAATATT
    ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT GTGGAGCTTC ATGACCCACC AAGAGGCCGC
    AACAAAGCCA CTGCCCACAA ACCAGCCCCT AGGAAAGAAC CAAGACCTAA CATCCCTTTG
    GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT GGCAAGATTG TGCCAAAGTC TCGGGAATTC
    ATTGAAACAG ATTCATCTAC ATCTGACTCC AACACAGATC AGGAAGAGAC CCTGCAAATC
    AAAGTCCTGC CTCCGTGCAT TATTTCTGGA GGTAATACTG CCAAATCCAA GGAAATCTGT
    GGTGCCAGCC TGACCCTCAG CACCTTAATG AGTAGCAGTG GCAGCAACAA CAACTTATCC
    ATCAGTAATG AAGAGCCAAC ATTTTCACCT ATTCCTGTCA TGCAAACTGA AATCCTGTCC
    CCTCTGCGAG ATCATGAGAA CCTGAAAAAC CTCTGGGTGA AGATTGACCT TGACTTACTC
    TCTAGAGTAC CTGGCCACAG CTCACTCCAT GCAGCACCTG CCAAGCCAGA CCACAAGGAG
    ACTGCCACAA AACCCAAGCG TCAGACAGCT GTCACAGCTG TGGAGAAACC AGCCCCTAAG
    GGCAAACGTA AGCACAAGCC AATAGAAGTT GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CCACAACTAT CTGCTTGCTC CCTCCTTGCA TCTCACCAGC CCCACCCCAC
    AAGCCTCCCA ACACTAGAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAGGAAGAA
    AAACTATTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGCCG CAGAAATGTC
    AGTGGCAATA ATGGTCCCTT TGGTCAAGAC AAAAACATCG CCATGACTGG ACAAATCACA
    TCTACCAAAC CTAAGAGAAC TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATATCGGTA
    AATGAGGGAG ACACTCCAAA AAAGGCATCC TCTGCCACCA TCACTGTCAC CAATACTGCT
    ATTGCCACTG CTACTGTCAC TGCTACTGCC ATTGTCACCA CCACTGTCAC AGCTACTGCC
    ACCGCCACGG CCACCACCAC AACTACTACC ACTACCATTT CCACCATCAC CTCTACCATC
    ACTACTGGCC TCATGGATAG CAGTCACCTG GAGATGACGT CCTGGGCGGC TCTGCCCCTT
    CTATCCAGCA GCAGCACTAA TGTCCGGAGA CCCAAGCTCA CTTTTGATGA CTCGGTTCAC
    AATGCTGATT ATTACATGCA AGAAGCTAAG AAGCTGAAGC ACAAAGCTGA TGCACTGTTC
    GAGAAATTTG GCAAAGCTGT GAATTATGCT GATGCCGCCC TCTCCTTCAC TGAATGTGGC
    AATGCCATGG AACGCGACCC TCTGGAAGCA AAGTCCCCAT ACACCATGTA CTCTGAGACT
    GTGGAGCTCC TCAGGTATGC AATGAGGCTG AAGAACTTTG CAAGTCCCTT GGCTTCGGAT
    GGGGACAAAA AGCTAGCAGT ACTATGCTAC CGATGTTTAT CACTCCTCTA TTTGAGAATG
    TTTAAGCTGA AGAAGGACCA TGCTATGAAG TACTCCAGAT CACTGATGGA ATATTTTAAG
    CAAAATGCTT CAAAAGTCGC ACAGATACCC TCTCCATGGG TAAGCAATGG AAAGAACACT
    CCATCCCCAG TGTCTCTCAA CAACGTCTCC CCCATCAACG CAATGGGGAA CTGTAACAAT
    GGCCCAGTCA CCATTCCCCA GCGCATTCAC CACATGGCTG CCAGCCACGT CAACATCACT
    AGCAATGTGT TACGGGGCTA TGAACACTGG GATATGGCCG ACAAACTGAC AAGAGAAAAC
    AAAGAATTCT TTGGTGATCT GGACACGCTG ATGGGGCCTC TGACCCAGCA CAGCAGCATG
    ACCAATCTTG TCCGCTACGT TCGCCAAGGA CTGTGTTGGC TGCGCATCGA TGCCCACTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162594.1
    CDS482..4387
    Translation

    Target ORF information:

    RefSeq Version NM_001169123.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169123.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAGATG
    ACCCATTCCT GGCCTACTCC TCTCACTTCC ATGCATACTG CTGGACACTC TGAGCAGAGC
    ACCTTTTCCA TCCCAGGACA GGAATCGCAG CATCTGACCC CAGGATTCAC CTTACAAAAG
    TGGAATGACC CAACCACCAG AGCTTCTACA AAGATGCTTG AGGATGACCT GAAGCTGAGC
    AGTGATGAAG ATGACCTTGA GCCTGTGAAG ACCTTGACCA CTCAGTGCAC TGCCACTGAG
    CTCTACCAGG CTGTTGAAAA GGCAAAACCT AGGAATAATC CTGTGAACCC ACCCTTGGCC
    ACTCCCCAGC CCCCACCTGC AGTGCAAGCC AGCGGGGGTT CTGGCAGCTC CAGCGAATCG
    GAGAGCAGCT CTGAGTCGGA TTCAGACACT GAAAGTAGCA CCACTGACAG CGAATCTAAT
    GAGGCACCTC GTGTGGCAAC TCCAGAGCCT GAGCCACCCT CAACCAACAA GTGGCAACTG
    GATAAATGGC TTAACAAAGT GACATCCCAG AACAAGTCTT TTATTTGTGG CCAAAATGAA
    ACACCCATGG AGACTATTTC TCTGCCTCCT CCAATCATCC AACCAATGGA AGTCCAGATG
    AAAGTGAAGA CGAATGCCAG TCAGGTCCCA GCTGAACCCA AAGAAAGGCC TCTCCTCAGT
    CTCATTAGGG AGAAAGCCCG TCCACGGCCC ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG
    AAGCATAAGT TGTCAACAAC TAGTGAGACA GTGTCTCAAA GGACAATTGG GAAAAAACAG
    CCCAAAAAAG TTGAGAAGAA CACCAGCACT GACGAGTTTA CCTGGCCCAA ACCAAATATT
    ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT GTGGAGCTTC ATGACCCACC AAGAGGCCGC
    AACAAAGCCA CTGCCCACAA ACCAGCCCCT AGGAAAGAAC CAAGACCTAA CATCCCTTTG
    GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT GGCAAGATTG TGCCAAAGTC TCGGGAATTC
    ATTGAAACAG ATTCATCTAC ATCTGACTCC AACACAGATC AGGAAGAGAC CCTGCAAATC
    AAAGTCCTGC CTCCGTGCAT TATTTCTGGA GGTAATACTG CCAAATCCAA GGAAATCTGT
    GGTGCCAGCC TGACCCTCAG CACCTTAATG AGTAGCAGTG GCAGCAACAA CAACTTATCC
    ATCAGTAATG AAGAGCCAAC ATTTTCACCT ATTCCTGTCA TGCAAACTGA AATCCTGTCC
    CCTCTGCGAG ATCATGAGAA CCTGAAAAAC CTCTGGGTGA AGATTGACCT TGACTTACTC
    TCTAGAGTAC CTGGCCACAG CTCACTCCAT GCAGCACCTG CCAAGCCAGA CCACAAGGAG
    ACTGCCACAA AACCCAAGCG TCAGACAGCT GTCACAGCTG TGGAGAAACC AGCCCCTAAG
    GGCAAACGTA AGCACAAGCC AATAGAAGTT GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CCACAACTAT CTGCTTGCTC CCTCCTTGCA TCTCACCAGC CCCACCCCAC
    AAGCCTCCCA ACACTAGAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAGGAAGAA
    AAACTATTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGCCG CAGAAATGTC
    AGTGGCAATA ATGGTCCCTT TGGTCAAGAC AAAAACATCG CCATGACTGG ACAAATCACA
    TCTACCAAAC CTAAGAGAAC TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATATCGGTA
    AATGAGGGAG ACACTCCAAA AAAGGCATCC TCTGCCACCA TCACTGTCAC CAATACTGCT
    ATTGCCACTG CTACTGTCAC TGCTACTGCC ATTGTCACCA CCACTGTCAC AGCTACTGCC
    ACCGCCACGG CCACCACCAC AACTACTACC ACTACCATTT CCACCATCAC CTCTACCATC
    ACTACTGGCC TCATGGATAG CAGTCACCTG GAGATGACGT CCTGGGCGGC TCTGCCCCTT
    CTATCCAGCA GCAGCACTAA TGTCCGGAGA CCCAAGCTCA CTTTTGATGA CTCGGTTCAC
    AATGCTGATT ATTACATGCA AGAAGCTAAG AAGCTGAAGC ACAAAGCTGA TGCACTGTTC
    GAGAAATTTG GCAAAGCTGT GAATTATGCT GATGCCGCCC TCTCCTTCAC TGAATGTGGC
    AATGCCATGG AACGCGACCC TCTGGAAGCA AAGTCCCCAT ACACCATGTA CTCTGAGACT
    GTGGAGCTCC TCAGGTATGC AATGAGGCTG AAGAACTTTG CAAGTCCCTT GGCTTCGGAT
    GGGGACAAAA AGCTAGCAGT ACTATGCTAC CGATGTTTAT CACTCCTCTA TTTGAGAATG
    TTTAAGCTGA AGAAGGACCA TGCTATGAAG TACTCCAGAT CACTGATGGA ATATTTTAAG
    CAAAATGCTT CAAAAGTCGC ACAGATACCC TCTCCATGGG TAAGCAATGG AAAGAACACT
    CCATCCCCAG TGTCTCTCAA CAACGTCTCC CCCATCAACG CAATGGGGAA CTGTAACAAT
    GGCCCAGTCA CCATTCCCCA GCGCATTCAC CACATGGCTG CCAGCCACGT CAACATCACT
    AGCAATGTGT TACGGGGCTA TGAACACTGG GATATGGCCG ACAAACTGAC AAGAGAAAAC
    AAAGAATTCT TTGGTGATCT GGACACGCTG ATGGGGCCTC TGACCCAGCA CAGCAGCATG
    ACCAATCTTG TCCGCTACGT TCGCCAAGGA CTGTGTTGGC TGCGCATCGA TGCCCACTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12312
    Clone ID Related Accession (Same CDS sequence) NM_001169123.2 , NM_001169123.1
    Accession Version NM_001169123.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U48436.1, BC132683.1 and AC231841.2. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAGATG
    ACCCATTCCT GGCCTACTCC TCTCACTTCC ATGCATACTG CTGGACACTC TGAGCAGAGC
    ACCTTTTCCA TCCCAGGACA GGAATCGCAG CATCTGACCC CAGGATTCAC CTTACAAAAG
    TGGAATGACC CAACCACCAG AGCTTCTACA AAGATGCTTG AGGATGACCT GAAGCTGAGC
    AGTGATGAAG ATGACCTTGA GCCTGTGAAG ACCTTGACCA CTCAGTGCAC TGCCACTGAG
    CTCTACCAGG CTGTTGAAAA GGCAAAACCT AGGAATAATC CTGTGAACCC ACCCTTGGCC
    ACTCCCCAGC CCCCACCTGC AGTGCAAGCC AGCGGGGGTT CTGGCAGCTC CAGCGAATCG
    GAGAGCAGCT CTGAGTCGGA TTCAGACACT GAAAGTAGCA CCACTGACAG CGAATCTAAT
    GAGGCACCTC GTGTGGCAAC TCCAGAGCCT GAGCCACCCT CAACCAACAA GTGGCAACTG
    GATAAATGGC TTAACAAAGT GACATCCCAG AACAAGTCTT TTATTTGTGG CCAAAATGAA
    ACACCCATGG AGACTATTTC TCTGCCTCCT CCAATCATCC AACCAATGGA AGTCCAGATG
    AAAGTGAAGA CGAATGCCAG TCAGGTCCCA GCTGAACCCA AAGAAAGGCC TCTCCTCAGT
    CTCATTAGGG AGAAAGCCCG TCCACGGCCC ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG
    AAGCATAAGT TGTCAACAAC TAGTGAGACA GTGTCTCAAA GGACAATTGG GAAAAAACAG
    CCCAAAAAAG TTGAGAAGAA CACCAGCACT GACGAGTTTA CCTGGCCCAA ACCAAATATT
    ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT GTGGAGCTTC ATGACCCACC AAGAGGCCGC
    AACAAAGCCA CTGCCCACAA ACCAGCCCCT AGGAAAGAAC CAAGACCTAA CATCCCTTTG
    GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT GGCAAGATTG TGCCAAAGTC TCGGGAATTC
    ATTGAAACAG ATTCATCTAC ATCTGACTCC AACACAGATC AGGAAGAGAC CCTGCAAATC
    AAAGTCCTGC CTCCGTGCAT TATTTCTGGA GGTAATACTG CCAAATCCAA GGAAATCTGT
    GGTGCCAGCC TGACCCTCAG CACCTTAATG AGTAGCAGTG GCAGCAACAA CAACTTATCC
    ATCAGTAATG AAGAGCCAAC ATTTTCACCT ATTCCTGTCA TGCAAACTGA AATCCTGTCC
    CCTCTGCGAG ATCATGAGAA CCTGAAAAAC CTCTGGGTGA AGATTGACCT TGACTTACTC
    TCTAGAGTAC CTGGCCACAG CTCACTCCAT GCAGCACCTG CCAAGCCAGA CCACAAGGAG
    ACTGCCACAA AACCCAAGCG TCAGACAGCT GTCACAGCTG TGGAGAAACC AGCCCCTAAG
    GGCAAACGTA AGCACAAGCC AATAGAAGTT GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CCACAACTAT CTGCTTGCTC CCTCCTTGCA TCTCACCAGC CCCACCCCAC
    AAGCCTCCCA ACACTAGAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAGGAAGAA
    AAACTATTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGCCG CAGAAATGTC
    AGTGGCAATA ATGGTCCCTT TGGTCAAGAC AAAAACATCG CCATGACTGG ACAAATCACA
    TCTACCAAAC CTAAGAGAAC TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATATCGGTA
    AATGAGGGAG ACACTCCAAA AAAGGCATCC TCTGCCACCA TCACTGTCAC CAATACTGCT
    ATTGCCACTG CTACTGTCAC TGCTACTGCC ATTGTCACCA CCACTGTCAC AGCTACTGCC
    ACCGCCACGG CCACCACCAC AACTACTACC ACTACCATTT CCACCATCAC CTCTACCATC
    ACTACTGGCC TCATGGATAG CAGTCACCTG GAGATGACGT CCTGGGCGGC TCTGCCCCTT
    CTATCCAGCA GCAGCACTAA TGTCCGGAGA CCCAAGCTCA CTTTTGATGA CTCGGTTCAC
    AATGCTGATT ATTACATGCA AGAAGCTAAG AAGCTGAAGC ACAAAGCTGA TGCACTGTTC
    GAGAAATTTG GCAAAGCTGT GAATTATGCT GATGCCGCCC TCTCCTTCAC TGAATGTGGC
    AATGCCATGG AACGCGACCC TCTGGAAGCA AAGTCCCCAT ACACCATGTA CTCTGAGACT
    GTGGAGCTCC TCAGGTATGC AATGAGGCTG AAGAACTTTG CAAGTCCCTT GGCTTCGGAT
    GGGGACAAAA AGCTAGCAGT ACTATGCTAC CGATGTTTAT CACTCCTCTA TTTGAGAATG
    TTTAAGCTGA AGAAGGACCA TGCTATGAAG TACTCCAGAT CACTGATGGA ATATTTTAAG
    CAAAATGCTT CAAAAGTCGC ACAGATACCC TCTCCATGGG TAAGCAATGG AAAGAACACT
    CCATCCCCAG TGTCTCTCAA CAACGTCTCC CCCATCAACG CAATGGGGAA CTGTAACAAT
    GGCCCAGTCA CCATTCCCCA GCGCATTCAC CACATGGCTG CCAGCCACGT CAACATCACT
    AGCAATGTGT TACGGGGCTA TGAACACTGG GATATGGCCG ACAAACTGAC AAGAGAAAAC
    AAAGAATTCT TTGGTGATCT GGACACGCTG ATGGGGCCTC TGACCCAGCA CAGCAGCATG
    ACCAATCTTG TCCGCTACGT TCGCCAAGGA CTGTGTTGGC TGCGCATCGA TGCCCACTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162594.1
    CDS480..4385
    Translation

    Target ORF information:

    RefSeq Version NM_001169123.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAGATG
    ACCCATTCCT GGCCTACTCC TCTCACTTCC ATGCATACTG CTGGACACTC TGAGCAGAGC
    ACCTTTTCCA TCCCAGGACA GGAATCGCAG CATCTGACCC CAGGATTCAC CTTACAAAAG
    TGGAATGACC CAACCACCAG AGCTTCTACA AAGATGCTTG AGGATGACCT GAAGCTGAGC
    AGTGATGAAG ATGACCTTGA GCCTGTGAAG ACCTTGACCA CTCAGTGCAC TGCCACTGAG
    CTCTACCAGG CTGTTGAAAA GGCAAAACCT AGGAATAATC CTGTGAACCC ACCCTTGGCC
    ACTCCCCAGC CCCCACCTGC AGTGCAAGCC AGCGGGGGTT CTGGCAGCTC CAGCGAATCG
    GAGAGCAGCT CTGAGTCGGA TTCAGACACT GAAAGTAGCA CCACTGACAG CGAATCTAAT
    GAGGCACCTC GTGTGGCAAC TCCAGAGCCT GAGCCACCCT CAACCAACAA GTGGCAACTG
    GATAAATGGC TTAACAAAGT GACATCCCAG AACAAGTCTT TTATTTGTGG CCAAAATGAA
    ACACCCATGG AGACTATTTC TCTGCCTCCT CCAATCATCC AACCAATGGA AGTCCAGATG
    AAAGTGAAGA CGAATGCCAG TCAGGTCCCA GCTGAACCCA AAGAAAGGCC TCTCCTCAGT
    CTCATTAGGG AGAAAGCCCG TCCACGGCCC ACTCAGAAAA TTCCAGAAAC AAAGGCTTTG
    AAGCATAAGT TGTCAACAAC TAGTGAGACA GTGTCTCAAA GGACAATTGG GAAAAAACAG
    CCCAAAAAAG TTGAGAAGAA CACCAGCACT GACGAGTTTA CCTGGCCCAA ACCAAATATT
    ACCAGCAGCA CTCCCAAAGA AAAAGAAAGT GTGGAGCTTC ATGACCCACC AAGAGGCCGC
    AACAAAGCCA CTGCCCACAA ACCAGCCCCT AGGAAAGAAC CAAGACCTAA CATCCCTTTG
    GCTCCCGAGA AGAAGAAGTA CAGAGGGCCT GGCAAGATTG TGCCAAAGTC TCGGGAATTC
    ATTGAAACAG ATTCATCTAC ATCTGACTCC AACACAGATC AGGAAGAGAC CCTGCAAATC
    AAAGTCCTGC CTCCGTGCAT TATTTCTGGA GGTAATACTG CCAAATCCAA GGAAATCTGT
    GGTGCCAGCC TGACCCTCAG CACCTTAATG AGTAGCAGTG GCAGCAACAA CAACTTATCC
    ATCAGTAATG AAGAGCCAAC ATTTTCACCT ATTCCTGTCA TGCAAACTGA AATCCTGTCC
    CCTCTGCGAG ATCATGAGAA CCTGAAAAAC CTCTGGGTGA AGATTGACCT TGACTTACTC
    TCTAGAGTAC CTGGCCACAG CTCACTCCAT GCAGCACCTG CCAAGCCAGA CCACAAGGAG
    ACTGCCACAA AACCCAAGCG TCAGACAGCT GTCACAGCTG TGGAGAAACC AGCCCCTAAG
    GGCAAACGTA AGCACAAGCC AATAGAAGTT GCAGAGAAGA TCCCTGAGAA GAAGCAGCGC
    CTGGAGGAGG CCACAACTAT CTGCTTGCTC CCTCCTTGCA TCTCACCAGC CCCACCCCAC
    AAGCCTCCCA ACACTAGAGA AAATAATTCA TCCAGGAGAG CAAATAGAAG AAAGGAAGAA
    AAACTATTTC CTCCTCCACT TTCCCCACTG CCAGAGGACC CTCCACGCCG CAGAAATGTC
    AGTGGCAATA ATGGTCCCTT TGGTCAAGAC AAAAACATCG CCATGACTGG ACAAATCACA
    TCTACCAAAC CTAAGAGAAC TGAAGGCAAA TTCTGTGCTA CTTTCAAAGG GATATCGGTA
    AATGAGGGAG ACACTCCAAA AAAGGCATCC TCTGCCACCA TCACTGTCAC CAATACTGCT
    ATTGCCACTG CTACTGTCAC TGCTACTGCC ATTGTCACCA CCACTGTCAC AGCTACTGCC
    ACCGCCACGG CCACCACCAC AACTACTACC ACTACCATTT CCACCATCAC CTCTACCATC
    ACTACTGGCC TCATGGATAG CAGTCACCTG GAGATGACGT CCTGGGCGGC TCTGCCCCTT
    CTATCCAGCA GCAGCACTAA TGTCCGGAGA CCCAAGCTCA CTTTTGATGA CTCGGTTCAC
    AATGCTGATT ATTACATGCA AGAAGCTAAG AAGCTGAAGC ACAAAGCTGA TGCACTGTTC
    GAGAAATTTG GCAAAGCTGT GAATTATGCT GATGCCGCCC TCTCCTTCAC TGAATGTGGC
    AATGCCATGG AACGCGACCC TCTGGAAGCA AAGTCCCCAT ACACCATGTA CTCTGAGACT
    GTGGAGCTCC TCAGGTATGC AATGAGGCTG AAGAACTTTG CAAGTCCCTT GGCTTCGGAT
    GGGGACAAAA AGCTAGCAGT ACTATGCTAC CGATGTTTAT CACTCCTCTA TTTGAGAATG
    TTTAAGCTGA AGAAGGACCA TGCTATGAAG TACTCCAGAT CACTGATGGA ATATTTTAAG
    CAAAATGCTT CAAAAGTCGC ACAGATACCC TCTCCATGGG TAAGCAATGG AAAGAACACT
    CCATCCCCAG TGTCTCTCAA CAACGTCTCC CCCATCAACG CAATGGGGAA CTGTAACAAT
    GGCCCAGTCA CCATTCCCCA GCGCATTCAC CACATGGCTG CCAGCCACGT CAACATCACT
    AGCAATGTGT TACGGGGCTA TGAACACTGG GATATGGCCG ACAAACTGAC AAGAGAAAAC
    AAAGAATTCT TTGGTGATCT GGACACGCTG ATGGGGCCTC TGACCCAGCA CAGCAGCATG
    ACCAATCTTG TCCGCTACGT TCGCCAAGGA CTGTGTTGGC TGCGCATCGA TGCCCACTTG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12937
    Clone ID Related Accession (Same CDS sequence) NM_001169122.1 , NM_001169122.2
    Accession Version NM_001169122.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U48436.1, L76569.1 and AC231841.2. Transcript Variant: This variant (2) has multiple differences, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L76569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGTCAG TGTCTTTCAA ATCGATGCTT GAGGATGACC TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCTGTGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAGGAATAAT CCTGTGAACC CACCCTTGGC CACTCCCCAG
    CCCCCACCTG CAGTGCAAGC CAGCGGGGGT TCTGGCAGCT CCAGCGAATC GGAGAGCAGC
    TCTGAGTCGG ATTCAGACAC TGAAAGTAGC ACCACTGACA GCGAATCTAA TGAGGCACCT
    CGTGTGGCAA CTCCAGAGCC TGAGCCACCC TCAACCAACA AGTGGCAACT GGATAAATGG
    CTTAACAAAG TGACATCCCA GAACAAGTCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACTATTT CTCTGCCTCC TCCAATCATC CAACCAATGG AAGTCCAGAT GAAAGTGAAG
    ACGAATGCCA GTCAGGTCCC AGCTGAACCC AAAGAAAGGC CTCTCCTCAG TCTCATTAGG
    GAGAAAGCCC GTCCACGGCC CACTCAGAAA ATTCCAGAAA CAAAGGCTTT GAAGCATAAG
    TTGTCAACAA CTAGTGAGAC AGTGTCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAAA
    GTTGAGAAGA ACACCAGCAC TGACGAGTTT ACCTGGCCCA AACCAAATAT TACCAGCAGC
    ACTCCCAAAG AAAAAGAAAG TGTGGAGCTT CATGACCCAC CAAGAGGCCG CAACAAAGCC
    ACTGCCCACA AACCAGCCCC TAGGAAAGAA CCAAGACCTA ACATCCCTTT GGCTCCCGAG
    AAGAAGAAGT ACAGAGGGCC TGGCAAGATT GTGCCAAAGT CTCGGGAATT CATTGAAACA
    GATTCATCTA CATCTGACTC CAACACAGAT CAGGAAGAGA CCCTGCAAAT CAAAGTCCTG
    CCTCCGTGCA TTATTTCTGG AGGTAATACT GCCAAATCCA AGGAAATCTG TGGTGCCAGC
    CTGACCCTCA GCACCTTAAT GAGTAGCAGT GGCAGCAACA ACAACTTATC CATCAGTAAT
    GAAGAGCCAA CATTTTCACC TATTCCTGTC ATGCAAACTG AAATCCTGTC CCCTCTGCGA
    GATCATGAGA ACCTGAAAAA CCTCTGGGTG AAGATTGACC TTGACTTACT CTCTAGAGTA
    CCTGGCCACA GCTCACTCCA TGCAGCACCT GCCAAGCCAG ACCACAAGGA GACTGCCACA
    AAACCCAAGC GTCAGACAGC TGTCACAGCT GTGGAGAAAC CAGCCCCTAA GGGCAAACGT
    AAGCACAAGC CAATAGAAGT TGCAGAGAAG ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG
    GCCACAACTA TCTGCTTGCT CCCTCCTTGC ATCTCACCAG CCCCACCCCA CAAGCCTCCC
    AACACTAGAG AAAATAATTC ATCCAGGAGA GCAAATAGAA GAAAGGAAGA AAAACTATTT
    CCTCCTCCAC TTTCCCCACT GCCAGAGGAC CCTCCACGCC GCAGAAATGT CAGTGGCAAT
    AATGGTCCCT TTGGTCAAGA CAAAAACATC GCCATGACTG GACAAATCAC ATCTACCAAA
    CCTAAGAGAA CTGAAGGCAA ATTCTGTGCT ACTTTCAAAG GGATATCGGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162593.1
    CDS480..4310
    Translation

    Target ORF information:

    RefSeq Version NM_001169122.1
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169122.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGTCAG TGTCTTTCAA ATCGATGCTT GAGGATGACC TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCTGTGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAGGAATAAT CCTGTGAACC CACCCTTGGC CACTCCCCAG
    CCCCCACCTG CAGTGCAAGC CAGCGGGGGT TCTGGCAGCT CCAGCGAATC GGAGAGCAGC
    TCTGAGTCGG ATTCAGACAC TGAAAGTAGC ACCACTGACA GCGAATCTAA TGAGGCACCT
    CGTGTGGCAA CTCCAGAGCC TGAGCCACCC TCAACCAACA AGTGGCAACT GGATAAATGG
    CTTAACAAAG TGACATCCCA GAACAAGTCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACTATTT CTCTGCCTCC TCCAATCATC CAACCAATGG AAGTCCAGAT GAAAGTGAAG
    ACGAATGCCA GTCAGGTCCC AGCTGAACCC AAAGAAAGGC CTCTCCTCAG TCTCATTAGG
    GAGAAAGCCC GTCCACGGCC CACTCAGAAA ATTCCAGAAA CAAAGGCTTT GAAGCATAAG
    TTGTCAACAA CTAGTGAGAC AGTGTCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAAA
    GTTGAGAAGA ACACCAGCAC TGACGAGTTT ACCTGGCCCA AACCAAATAT TACCAGCAGC
    ACTCCCAAAG AAAAAGAAAG TGTGGAGCTT CATGACCCAC CAAGAGGCCG CAACAAAGCC
    ACTGCCCACA AACCAGCCCC TAGGAAAGAA CCAAGACCTA ACATCCCTTT GGCTCCCGAG
    AAGAAGAAGT ACAGAGGGCC TGGCAAGATT GTGCCAAAGT CTCGGGAATT CATTGAAACA
    GATTCATCTA CATCTGACTC CAACACAGAT CAGGAAGAGA CCCTGCAAAT CAAAGTCCTG
    CCTCCGTGCA TTATTTCTGG AGGTAATACT GCCAAATCCA AGGAAATCTG TGGTGCCAGC
    CTGACCCTCA GCACCTTAAT GAGTAGCAGT GGCAGCAACA ACAACTTATC CATCAGTAAT
    GAAGAGCCAA CATTTTCACC TATTCCTGTC ATGCAAACTG AAATCCTGTC CCCTCTGCGA
    GATCATGAGA ACCTGAAAAA CCTCTGGGTG AAGATTGACC TTGACTTACT CTCTAGAGTA
    CCTGGCCACA GCTCACTCCA TGCAGCACCT GCCAAGCCAG ACCACAAGGA GACTGCCACA
    AAACCCAAGC GTCAGACAGC TGTCACAGCT GTGGAGAAAC CAGCCCCTAA GGGCAAACGT
    AAGCACAAGC CAATAGAAGT TGCAGAGAAG ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG
    GCCACAACTA TCTGCTTGCT CCCTCCTTGC ATCTCACCAG CCCCACCCCA CAAGCCTCCC
    AACACTAGAG AAAATAATTC ATCCAGGAGA GCAAATAGAA GAAAGGAAGA AAAACTATTT
    CCTCCTCCAC TTTCCCCACT GCCAGAGGAC CCTCCACGCC GCAGAAATGT CAGTGGCAAT
    AATGGTCCCT TTGGTCAAGA CAAAAACATC GCCATGACTG GACAAATCAC ATCTACCAAA
    CCTAAGAGAA CTGAAGGCAA ATTCTGTGCT ACTTTCAAAG GGATATCGGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12937
    Clone ID Related Accession (Same CDS sequence) NM_001169122.1 , NM_001169122.2
    Accession Version NM_001169122.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product AF4/FMR2 family member 2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002368.1, U48436.1, L76569.1 and AC231841.2. On May 31, 2019 this sequence version replaced NM_001169122.1. Transcript Variant: This variant (2) has multiple differences, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L76569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGTCAG TGTCTTTCAA ATCGATGCTT GAGGATGACC TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCTGTGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAGGAATAAT CCTGTGAACC CACCCTTGGC CACTCCCCAG
    CCCCCACCTG CAGTGCAAGC CAGCGGGGGT TCTGGCAGCT CCAGCGAATC GGAGAGCAGC
    TCTGAGTCGG ATTCAGACAC TGAAAGTAGC ACCACTGACA GCGAATCTAA TGAGGCACCT
    CGTGTGGCAA CTCCAGAGCC TGAGCCACCC TCAACCAACA AGTGGCAACT GGATAAATGG
    CTTAACAAAG TGACATCCCA GAACAAGTCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACTATTT CTCTGCCTCC TCCAATCATC CAACCAATGG AAGTCCAGAT GAAAGTGAAG
    ACGAATGCCA GTCAGGTCCC AGCTGAACCC AAAGAAAGGC CTCTCCTCAG TCTCATTAGG
    GAGAAAGCCC GTCCACGGCC CACTCAGAAA ATTCCAGAAA CAAAGGCTTT GAAGCATAAG
    TTGTCAACAA CTAGTGAGAC AGTGTCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAAA
    GTTGAGAAGA ACACCAGCAC TGACGAGTTT ACCTGGCCCA AACCAAATAT TACCAGCAGC
    ACTCCCAAAG AAAAAGAAAG TGTGGAGCTT CATGACCCAC CAAGAGGCCG CAACAAAGCC
    ACTGCCCACA AACCAGCCCC TAGGAAAGAA CCAAGACCTA ACATCCCTTT GGCTCCCGAG
    AAGAAGAAGT ACAGAGGGCC TGGCAAGATT GTGCCAAAGT CTCGGGAATT CATTGAAACA
    GATTCATCTA CATCTGACTC CAACACAGAT CAGGAAGAGA CCCTGCAAAT CAAAGTCCTG
    CCTCCGTGCA TTATTTCTGG AGGTAATACT GCCAAATCCA AGGAAATCTG TGGTGCCAGC
    CTGACCCTCA GCACCTTAAT GAGTAGCAGT GGCAGCAACA ACAACTTATC CATCAGTAAT
    GAAGAGCCAA CATTTTCACC TATTCCTGTC ATGCAAACTG AAATCCTGTC CCCTCTGCGA
    GATCATGAGA ACCTGAAAAA CCTCTGGGTG AAGATTGACC TTGACTTACT CTCTAGAGTA
    CCTGGCCACA GCTCACTCCA TGCAGCACCT GCCAAGCCAG ACCACAAGGA GACTGCCACA
    AAACCCAAGC GTCAGACAGC TGTCACAGCT GTGGAGAAAC CAGCCCCTAA GGGCAAACGT
    AAGCACAAGC CAATAGAAGT TGCAGAGAAG ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG
    GCCACAACTA TCTGCTTGCT CCCTCCTTGC ATCTCACCAG CCCCACCCCA CAAGCCTCCC
    AACACTAGAG AAAATAATTC ATCCAGGAGA GCAAATAGAA GAAAGGAAGA AAAACTATTT
    CCTCCTCCAC TTTCCCCACT GCCAGAGGAC CCTCCACGCC GCAGAAATGT CAGTGGCAAT
    AATGGTCCCT TTGGTCAAGA CAAAAACATC GCCATGACTG GACAAATCAC ATCTACCAAA
    CCTAAGAGAA CTGAAGGCAA ATTCTGTGCT ACTTTCAAAG GGATATCGGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162593.1
    CDS482..4312
    Translation

    Target ORF information:

    RefSeq Version NM_001169122.2
    Organism Homo sapiens(human)
    Definition Homo sapiens AF4/FMR2 family member 2 (AFF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169122.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCTAT TCGACTTTTT CAGAGACTGG GACTTGGAGC AGCAGTGTCA CTATGAACAA 
    GACCGTAGTG CACTTAAAAA AAGGGAATGG GAGCGGAGGA ATCAAGAAGT CCAGCAAGAA
    GACGATCTCT TTTCTTCAGG CTTTGATCTT TTTGGGGAGC CATACAAGAC AAACAAAGGT
    GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT ATGATGAAAT GAAGAATTTG
    CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC CAAAGAATTC TGTGCCCCAG
    AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC AAAAGAACAG AATAATTCCA
    CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC CACCTTCTGT TGTGATACTG
    AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG AGTGGTCACG TGATAGTCAT
    AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC CAAACAAGAT GCAGACTTTG
    ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG TCTACCCAGC TGAACAGCCC
    CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG AAGACAGTAA CCCTAATTCT
    AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT CACCGGAAGA ATCTGAATTC
    GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT TATTAGCTCC TAGCAGTGGC
    CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA CAAGCATGGG GCAGCAAAAG
    CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG CACCGGACAT CTCACCAACA
    CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC TGTCATTTGG AACACTCTTG
    GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC CAAAGTTCAC CATCCTCCAA
    ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG AAGAAATCTT GCGGGAATCG
    CAGCATCTGA CCCCAGGATT CACCTTACAA AAGTGGAATG ACCCAACCAC CAGAGCTTCT
    ACAAAGTCAG TGTCTTTCAA ATCGATGCTT GAGGATGACC TGAAGCTGAG CAGTGATGAA
    GATGACCTTG AGCCTGTGAA GACCTTGACC ACTCAGTGCA CTGCCACTGA GCTCTACCAG
    GCTGTTGAAA AGGCAAAACC TAGGAATAAT CCTGTGAACC CACCCTTGGC CACTCCCCAG
    CCCCCACCTG CAGTGCAAGC CAGCGGGGGT TCTGGCAGCT CCAGCGAATC GGAGAGCAGC
    TCTGAGTCGG ATTCAGACAC TGAAAGTAGC ACCACTGACA GCGAATCTAA TGAGGCACCT
    CGTGTGGCAA CTCCAGAGCC TGAGCCACCC TCAACCAACA AGTGGCAACT GGATAAATGG
    CTTAACAAAG TGACATCCCA GAACAAGTCT TTTATTTGTG GCCAAAATGA AACACCCATG
    GAGACTATTT CTCTGCCTCC TCCAATCATC CAACCAATGG AAGTCCAGAT GAAAGTGAAG
    ACGAATGCCA GTCAGGTCCC AGCTGAACCC AAAGAAAGGC CTCTCCTCAG TCTCATTAGG
    GAGAAAGCCC GTCCACGGCC CACTCAGAAA ATTCCAGAAA CAAAGGCTTT GAAGCATAAG
    TTGTCAACAA CTAGTGAGAC AGTGTCTCAA AGGACAATTG GGAAAAAACA GCCCAAAAAA
    GTTGAGAAGA ACACCAGCAC TGACGAGTTT ACCTGGCCCA AACCAAATAT TACCAGCAGC
    ACTCCCAAAG AAAAAGAAAG TGTGGAGCTT CATGACCCAC CAAGAGGCCG CAACAAAGCC
    ACTGCCCACA AACCAGCCCC TAGGAAAGAA CCAAGACCTA ACATCCCTTT GGCTCCCGAG
    AAGAAGAAGT ACAGAGGGCC TGGCAAGATT GTGCCAAAGT CTCGGGAATT CATTGAAACA
    GATTCATCTA CATCTGACTC CAACACAGAT CAGGAAGAGA CCCTGCAAAT CAAAGTCCTG
    CCTCCGTGCA TTATTTCTGG AGGTAATACT GCCAAATCCA AGGAAATCTG TGGTGCCAGC
    CTGACCCTCA GCACCTTAAT GAGTAGCAGT GGCAGCAACA ACAACTTATC CATCAGTAAT
    GAAGAGCCAA CATTTTCACC TATTCCTGTC ATGCAAACTG AAATCCTGTC CCCTCTGCGA
    GATCATGAGA ACCTGAAAAA CCTCTGGGTG AAGATTGACC TTGACTTACT CTCTAGAGTA
    CCTGGCCACA GCTCACTCCA TGCAGCACCT GCCAAGCCAG ACCACAAGGA GACTGCCACA
    AAACCCAAGC GTCAGACAGC TGTCACAGCT GTGGAGAAAC CAGCCCCTAA GGGCAAACGT
    AAGCACAAGC CAATAGAAGT TGCAGAGAAG ATCCCTGAGA AGAAGCAGCG CCTGGAGGAG
    GCCACAACTA TCTGCTTGCT CCCTCCTTGC ATCTCACCAG CCCCACCCCA CAAGCCTCCC
    AACACTAGAG AAAATAATTC ATCCAGGAGA GCAAATAGAA GAAAGGAAGA AAAACTATTT
    CCTCCTCCAC TTTCCCCACT GCCAGAGGAC CCTCCACGCC GCAGAAATGT CAGTGGCAAT
    AATGGTCCCT TTGGTCAAGA CAAAAACATC GCCATGACTG GACAAATCAC ATCTACCAAA
    CCTAAGAGAA CTGAAGGCAA ATTCTGTGCT ACTTTCAAAG GGATATCGGA GGGAGACACT
    CCAAAAAAGG CATCCTCTGC CACCATCACT GTCACCAATA CTGCTATTGC CACTGCTACT
    GTCACTGCTA CTGCCATTGT CACCACCACT GTCACAGCTA CTGCCACCGC CACGGCCACC
    ACCACAACTA CTACCACTAC CATTTCCACC ATCACCTCTA CCATCACTAC TGGCCTCATG
    GATAGCAGTC ACCTGGAGAT GACGTCCTGG GCGGCTCTGC CCCTTCTATC CAGCAGCAGC
    ACTAATGTCC GGAGACCCAA GCTCACTTTT GATGACTCGG TTCACAATGC TGATTATTAC
    ATGCAAGAAG CTAAGAAGCT GAAGCACAAA GCTGATGCAC TGTTCGAGAA ATTTGGCAAA
    GCTGTGAATT ATGCTGATGC CGCCCTCTCC TTCACTGAAT GTGGCAATGC CATGGAACGC
    GACCCTCTGG AAGCAAAGTC CCCATACACC ATGTACTCTG AGACTGTGGA GCTCCTCAGG
    TATGCAATGA GGCTGAAGAA CTTTGCAAGT CCCTTGGCTT CGGATGGGGA CAAAAAGCTA
    GCAGTACTAT GCTACCGATG TTTATCACTC CTCTATTTGA GAATGTTTAA GCTGAAGAAG
    GACCATGCTA TGAAGTACTC CAGATCACTG ATGGAATATT TTAAGCAAAA TGCTTCAAAA
    GTCGCACAGA TACCCTCTCC ATGGGTAAGC AATGGAAAGA ACACTCCATC CCCAGTGTCT
    CTCAACAACG TCTCCCCCAT CAACGCAATG GGGAACTGTA ACAATGGCCC AGTCACCATT
    CCCCAGCGCA TTCACCACAT GGCTGCCAGC CACGTCAACA TCACTAGCAA TGTGTTACGG
    GGCTATGAAC ACTGGGATAT GGCCGACAAA CTGACAAGAG AAAACAAAGA ATTCTTTGGT
    GATCTGGACA CGCTGATGGG GCCTCTGACC CAGCACAGCA GCATGACCAA TCTTGTCCGC
    TACGTTCGCC AAGGACTGTG TTGGCTGCGC ATCGATGCCC ACTTGTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.