MAN2B2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2B2
Entrez Gene ID	23324
Full Name	mannosidase alpha class 2B member 2
General protein information	Preferred Names mannosidase alpha class 2B member 2 Names epididymis-specific alpha-mannosidase core-specific lysosomal alpha-1,6-Mannosidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_015274.2	NP_056089.1	epididymis-specific alpha-mannosidase isoform 1 precursor
NM_001292038.1	NP_001278967.1	epididymis-specific alpha-mannosidase isoform 2 precursor
NM_001292038.2	NP_001278967.1	epididymis-specific alpha-mannosidase isoform 2 precursor
NM_015274.3	NP_056089.1	epididymis-specific alpha-mannosidase isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B2	XP_003310289.1
	MAN2B2	XP_002804053.1
Bos taurus (cattle)	LOC532207	XP_003586284.2
Gallus gallus (chicken)	MAN2B2	XP_420805.2
Canis lupus familiaris (dog)	LOC100855835	XP_005618789.1
Bos taurus (cattle)	MAN2B2	XP_002688458.1
Mus musculus (house mouse)	Man2b2	NP_032576.2
Canis lupus familiaris (dog)	MAN2B2	XP_005642316.1
Rattus norvegicus (Norway rat)	Man2b2	NP_001128443.1
Homo sapiens (human)	MAN2B2	NP_056089.1
Danio rerio (zebrafish)	man2b2	XP_001337519.3
Xenopus tropicalis (tropical clawed frog)	man2b2	NP_001106515.1

Gene Ontology
Bibliography

Related articles in PubMed

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanch? H, Schreiber S, Nebel A
Mutation research694(1-2)13-9(2010 Dec)

Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties.
Venkatesan M, Kuntz DA, Rose DR
Protein science : a publication of the Protein Society18(11)2242-51(2009 Nov)

Characterization of a human core-specific lysosomal {alpha}1,6-mannosidase involved in N-glycan catabolism.
Park C, Meng L, Stanton LH, Collins RE, Mast SW, Yi X, Strachan H, Moremen KW
The Journal of biological chemistry280(44)37204-16(2005 Nov)

Purification and properties of major alpha-D-mannosidase in the luminal fluid of porcine epididymis.
Jin YZ, Dacheux F, Dacheux JL, Bannai S, Sugita Y, Okamura N
Biochimica et biophysica acta1432(2)382-92(1999 Jul)

Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia.
Hiramoto S, Tamba M, Kiuchi S, Jin YZ, Bannai S, Sugita Y, Dacheux F, Dacheux JL, Yoshida M, Okamura N
Biochemical and biophysical research communications241(2)439-45(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Expressed MAN2B1 and MAN2B2 in Drosophila S2 cells and functionally characterized them. MAN2B1 and MAN2B2 were significantly inhibited by the class II alpha-mannosidase inhibitors, swainsonine and mannostatin A.
Title: Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties.

The following MAN2B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24659	NM_001292038.1 Latest version!	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu108262	NM_001292038.2 Latest version!	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu108609	NM_015274.3 Latest version!	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu24671	NM_015274.2 Latest version!	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2B2 ( NM_001292038.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278967.1 Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24659
Clone ID Related Accession (Same CDS sequence)	NM_001292038.1
Accession Version	NM_001292038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-08
Organism	Homo sapiens(human)
Product	epididymis-specific alpha-mannosidase isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE243063.1, AK301473.1, AL833071.2, BC033307.1, BM730115.1, BX350248.2 and AK025803.1. Transcript Variant: This variant (2) uses alternate in-frame splice sites in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK301473.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGCTCGGTGT CTCGGTGCAG TATGCCACGC TGGGCGACTA CTTCCGTGCC 
CTGCACGCTC TCAATGTCAC CTGGCGTGTC CGCGACCACC ACGACTTCCT GCCCTATTCC 
ACAGAACCAT TCCAGGCCTG GACGGGCTTC TACACGTCCC GCAGCTCACT GAAGGGGCTG 
GCCCGGCGAG CCAGCGCCTT GTTGTATGCC GGGGAGTCCA TGTTCACACG CTACCTGTGG 
CCGGCCCCCC GTGGGCATCT GGACCCCACC TGGGCCCTGC AGCAGCTCCA GCAGCTTCGC 
TGGGCCGTCT CCGAGGTCCA GCACCATGAT GCCATCACTG GGACTGAGTC CCCCAAGGTG 
AGAGACATGT ACGCAACGCA CCTGGCCTCG GGGATGCTGG GCATGCGCAA GCTGATGGCC 
TCCATCGTCC TAGATGAGCT CCAGCCCCAG GCACCCATGG CGGCCAGCTC CGATGCAGGA 
CCTGCAGGAC ATTTTGCCTC GGTCTACAAC CCGCTGGCCT GGACGGTCAC CACCATCGTC 
ACCCTGACTG TTGGTTTCCC TGGAGTCCGC GTCACAGATG AGGCGGGCCA CCCAGTGCCC 
TCGCAGATCC AGAACTCAAC AGAGACCCCA TCTGCGTATG ACCTGCTTAT TCTGACCACA 
ATCCCAGGCC TCAGTTACCG GCACTACAAC ATCAGACCCA CTGCAGGGGC CCAAGAGGGC 
ACCCAGGAGC CGGCTGCCAC TGTGGCGAGC ACCCTTCAAT TTGGCCGCAG GCTGAGGAGA 
CGCACCAGCC ATGCGGGCAG GTACTTGGTG CCTGTGGCAA ACGACTGCTA CATTGTGCTG 
CTCGACCAGG ATACCAACCT GATGCACAGC ATCTGGGAGA GACAGAGTAA CCGAACGGTG 
CGCGTGACCC AGGAATTCCT GGAGTACCAC GTCAACGGGG ATGTGAAACA GGGCCCCATT 
TCCGATAACT ACCTGTTCAC ACCGGGCAAG GCCGCGGTGC CTGCGTGGGA AGCTGTGGAA 
ATGGAGATTG TGGCGGGACA GCTTGTGACT GAGATCCGGC AGTACTTCTA CAGGAACATG 
ACAGCACAGA ATTACACGTA TGCAATCCGC TCCCGGCTCA CCCATGTGCC GCAGGGCCAT 
GACGGGGAGC TGCTCTGCCA CCGGATAGAG CAGGAGTACC AAGCCGGCCC CCTGGAGCTG 
AACCGTGAGG CTGTCCTGAG GACCAGCACC AACCTAAACA GCCAGCAGGT CATCTACTCA 
GACAACAACG GCTACCAGAT GCAGCGGAGG CCCTACGTTT CCTATGTGAA CAACAGCATC 
GCCCGGAATT ACTACCCCAT GGTTCAGTCG GCCTTCATGG AGGATGGCAA AAGCAGGCTT 
GTGTTGCTGT CGGAGCGGGC ACATGGCATC TCCAGCCAAG GGAATGGGCA GGTGGAGGTC 
ATGCTCCACC GGCGGCTGTG GAACAACTTC GACTGGGACC TGGGCTACAA CCTCACGCTG 
AACGACACCT CAGTCGTCCA CCCAGTGCTC TGGCTTCTGC TGGGATCCTG GTCCCTCACC 
ACTGCCCTGC GCCAGAGGAG CGCACTGGCG CTGCAGCACA GGCCCGTGGT GCTGTTCGGA 
GACCTCGCTG GGACTGCGCC GAAGCTCCCA GGACCCCAGC AGCAAGAGGC CGTGACGCTG 
CCCCCGAATC TTCACCTGCA GATCCTGAGC ATCCCTGGCT GGCGCTACAG CTCCAACCAC 
ACGGAGCACT CTCAGAATCT CCGGAAAGGC CATCGAGGGG AAGCCCAGGC TGACCTCCGC 
CGTGTCCTGC TGCGGCTCTA CCACCTGTAT GAAGTGGGCG AGGACCCAGT CCTGTCTCAG 
CCAGTGACAG TGAATCTGGA GGCTGTGCTG CAGGCGCTGG GGTCCGTGGT GGCAGTGGAG 
GAGCGCTCGC TCACAGGGAC CTGGGATTTG AGCATGCTGC ACCGCTGGAG CTGGAGGACG 
GGGCCTGGCC GCCACAGAGG TGACACCACC TCTCCCTCGA GGCCACCAGG AGGCCCCATC 
ATCACCGTCC ACCCAAAGGA AATCCGGACG TTCTTTATTC ACTTTCAACA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278967.1
CDS	38..2914
Translation	MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPQDGVYPNMSEPVTPANINLYAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGMRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYNIRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNGDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ

Target ORF information:

RefSeq Version	NM_001292038.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292038.1

ORF Insert Sequence:

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGCTCGGTGT CTCGGTGCAG TATGCCACGC TGGGCGACTA CTTCCGTGCC 
CTGCACGCTC TCAATGTCAC CTGGCGTGTC CGCGACCACC ACGACTTCCT GCCCTATTCC 
ACAGAACCAT TCCAGGCCTG GACGGGCTTC TACACGTCCC GCAGCTCACT GAAGGGGCTG 
GCCCGGCGAG CCAGCGCCTT GTTGTATGCC GGGGAGTCCA TGTTCACACG CTACCTGTGG 
CCGGCCCCCC GTGGGCATCT GGACCCCACC TGGGCCCTGC AGCAGCTCCA GCAGCTTCGC 
TGGGCCGTCT CCGAGGTCCA GCACCATGAT GCCATCACTG GGACTGAGTC CCCCAAGGTG 
AGAGACATGT ACGCAACGCA CCTGGCCTCG GGGATGCTGG GCATGCGCAA GCTGATGGCC 
TCCATCGTCC TAGATGAGCT CCAGCCCCAG GCACCCATGG CGGCCAGCTC CGATGCAGGA 
CCTGCAGGAC ATTTTGCCTC GGTCTACAAC CCGCTGGCCT GGACGGTCAC CACCATCGTC 
ACCCTGACTG TTGGTTTCCC TGGAGTCCGC GTCACAGATG AGGCGGGCCA CCCAGTGCCC 
TCGCAGATCC AGAACTCAAC AGAGACCCCA TCTGCGTATG ACCTGCTTAT TCTGACCACA 
ATCCCAGGCC TCAGTTACCG GCACTACAAC ATCAGACCCA CTGCAGGGGC CCAAGAGGGC 
ACCCAGGAGC CGGCTGCCAC TGTGGCGAGC ACCCTTCAAT TTGGCCGCAG GCTGAGGAGA 
CGCACCAGCC ATGCGGGCAG GTACTTGGTG CCTGTGGCAA ACGACTGCTA CATTGTGCTG 
CTCGACCAGG ATACCAACCT GATGCACAGC ATCTGGGAGA GACAGAGTAA CCGAACGGTG 
CGCGTGACCC AGGAATTCCT GGAGTACCAC GTCAACGGGG ATGTGAAACA GGGCCCCATT 
TCCGATAACT ACCTGTTCAC ACCGGGCAAG GCCGCGGTGC CTGCGTGGGA AGCTGTGGAA 
ATGGAGATTG TGGCGGGACA GCTTGTGACT GAGATCCGGC AGTACTTCTA CAGGAACATG 
ACAGCACAGA ATTACACGTA TGCAATCCGC TCCCGGCTCA CCCATGTGCC GCAGGGCCAT 
GACGGGGAGC TGCTCTGCCA CCGGATAGAG CAGGAGTACC AAGCCGGCCC CCTGGAGCTG 
AACCGTGAGG CTGTCCTGAG GACCAGCACC AACCTAAACA GCCAGCAGGT CATCTACTCA 
GACAACAACG GCTACCAGAT GCAGCGGAGG CCCTACGTTT CCTATGTGAA CAACAGCATC 
GCCCGGAATT ACTACCCCAT GGTTCAGTCG GCCTTCATGG AGGATGGCAA AAGCAGGCTT 
GTGTTGCTGT CGGAGCGGGC ACATGGCATC TCCAGCCAAG GGAATGGGCA GGTGGAGGTC 
ATGCTCCACC GGCGGCTGTG GAACAACTTC GACTGGGACC TGGGCTACAA CCTCACGCTG 
AACGACACCT CAGTCGTCCA CCCAGTGCTC TGGCTTCTGC TGGGATCCTG GTCCCTCACC 
ACTGCCCTGC GCCAGAGGAG CGCACTGGCG CTGCAGCACA GGCCCGTGGT GCTGTTCGGA 
GACCTCGCTG GGACTGCGCC GAAGCTCCCA GGACCCCAGC AGCAAGAGGC CGTGACGCTG 
CCCCCGAATC TTCACCTGCA GATCCTGAGC ATCCCTGGCT GGCGCTACAG CTCCAACCAC 
ACGGAGCACT CTCAGAATCT CCGGAAAGGC CATCGAGGGG AAGCCCAGGC TGACCTCCGC 
CGTGTCCTGC TGCGGCTCTA CCACCTGTAT GAAGTGGGCG AGGACCCAGT CCTGTCTCAG 
CCAGTGACAG TGAATCTGGA GGCTGTGCTG CAGGCGCTGG GGTCCGTGGT GGCAGTGGAG 
GAGCGCTCGC TCACAGGGAC CTGGGATTTG AGCATGCTGC ACCGCTGGAG CTGGAGGACG 
GGGCCTGGCC GCCACAGAGG TGACACCACC TCTCCCTCGA GGCCACCAGG AGGCCCCATC 
ATCACCGTCC ACCCAAAGGA AATCCGGACG TTCTTTATTC ACTTTCAACA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B2 ( NM_001292038.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278967.1 Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108262
Clone ID Related Accession (Same CDS sequence)	NM_001292038.2
Accession Version	NM_001292038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	epididymis-specific alpha-mannosidase isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093323.3. On Jun 1, 2019 this sequence version replaced NM_001292038.1. Transcript Variant: This variant (2) uses alternate in-frame splice sites in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK301473.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGCTCGGTGT CTCGGTGCAG TATGCCACGC TGGGCGACTA CTTCCGTGCC 
CTGCACGCTC TCAATGTCAC CTGGCGTGTC CGCGACCACC ACGACTTCCT GCCCTATTCC 
ACAGAACCAT TCCAGGCCTG GACGGGCTTC TACACGTCCC GCAGCTCACT GAAGGGGCTG 
GCCCGGCGAG CCAGCGCCTT GTTGTATGCC GGGGAGTCCA TGTTCACACG CTACCTGTGG 
CCGGCCCCCC GTGGGCATCT GGACCCCACC TGGGCCCTGC AGCAGCTCCA GCAGCTTCGC 
TGGGCCGTCT CCGAGGTCCA GCACCATGAT GCCATCACTG GGACTGAGTC CCCCAAGGTG 
AGAGACATGT ACGCAACGCA CCTGGCCTCG GGGATGCTGG GCATGCGCAA GCTGATGGCC 
TCCATCGTCC TAGATGAGCT CCAGCCCCAG GCACCCATGG CGGCCAGCTC CGATGCAGGA 
CCTGCAGGAC ATTTTGCCTC GGTCTACAAC CCGCTGGCCT GGACGGTCAC CACCATCGTC 
ACCCTGACTG TTGGTTTCCC TGGAGTCCGC GTCACAGATG AGGCGGGCCA CCCAGTGCCC 
TCGCAGATCC AGAACTCAAC AGAGACCCCA TCTGCGTATG ACCTGCTTAT TCTGACCACA 
ATCCCAGGCC TCAGTTACCG GCACTACAAC ATCAGACCCA CTGCAGGGGC CCAAGAGGGC 
ACCCAGGAGC CGGCTGCCAC TGTGGCGAGC ACCCTTCAAT TTGGCCGCAG GCTGAGGAGA 
CGCACCAGCC ATGCGGGCAG GTACTTGGTG CCTGTGGCAA ACGACTGCTA CATTGTGCTG 
CTCGACCAGG ATACCAACCT GATGCACAGC ATCTGGGAGA GACAGAGTAA CCGAACGGTG 
CGCGTGACCC AGGAATTCCT GGAGTACCAC GTCAACGGGG ATGTGAAACA GGGCCCCATT 
TCCGATAACT ACCTGTTCAC ACCGGGCAAG GCCGCGGTGC CTGCGTGGGA AGCTGTGGAA 
ATGGAGATTG TGGCGGGACA GCTTGTGACT GAGATCCGGC AGTACTTCTA CAGGAACATG 
ACAGCACAGA ATTACACGTA TGCAATCCGC TCCCGGCTCA CCCATGTGCC GCAGGGCCAT 
GACGGGGAGC TGCTCTGCCA CCGGATAGAG CAGGAGTACC AAGCCGGCCC CCTGGAGCTG 
AACCGTGAGG CTGTCCTGAG GACCAGCACC AACCTAAACA GCCAGCAGGT CATCTACTCA 
GACAACAACG GCTACCAGAT GCAGCGGAGG CCCTACGTTT CCTATGTGAA CAACAGCATC 
GCCCGGAATT ACTACCCCAT GGTTCAGTCG GCCTTCATGG AGGATGGCAA AAGCAGGCTT 
GTGTTGCTGT CGGAGCGGGC ACATGGCATC TCCAGCCAAG GGAATGGGCA GGTGGAGGTC 
ATGCTCCACC GGCGGCTGTG GAACAACTTC GACTGGGACC TGGGCTACAA CCTCACGCTG 
AACGACACCT CAGTCGTCCA CCCAGTGCTC TGGCTTCTGC TGGGATCCTG GTCCCTCACC 
ACTGCCCTGC GCCAGAGGAG CGCACTGGCG CTGCAGCACA GGCCCGTGGT GCTGTTCGGA 
GACCTCGCTG GGACTGCGCC GAAGCTCCCA GGACCCCAGC AGCAAGAGGC CGTGACGCTG 
CCCCCGAATC TTCACCTGCA GATCCTGAGC ATCCCTGGCT GGCGCTACAG CTCCAACCAC 
ACGGAGCACT CTCAGAATCT CCGGAAAGGC CATCGAGGGG AAGCCCAGGC TGACCTCCGC 
CGTGTCCTGC TGCGGCTCTA CCACCTATAT GAAGTGGGCG AGGACCCAGT CCTGTCTCAG 
CCAGTGACAG TGAATCTGGA GGCTGTGCTG CAGGCGCTGG GGTCCGTGGT GGCAGTGGAG 
GAGCGCTCGC TCACAGGGAC CTGGGATTTG AGCATGCTGC ACCGCTGGAG CTGGAGGACG 
GGGCCTGGCC GCCACAGAGG TGACACCACC TCTCCCTCGA GGCCACCAGG AGGCCCCATC 
ATCACCGTCC ACCCAAAGGA AATCCGGACG TTCTTTATTC ACTTTCAACA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278967.1
CDS	23..2899
Translation	MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPQDGVYPNMSEPVTPANINLYAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGMRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYNIRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNGDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ

Target ORF information:

RefSeq Version	NM_001292038.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292038.2

ORF Insert Sequence:

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGCTCGGTGT CTCGGTGCAG TATGCCACGC TGGGCGACTA CTTCCGTGCC 
CTGCACGCTC TCAATGTCAC CTGGCGTGTC CGCGACCACC ACGACTTCCT GCCCTATTCC 
ACAGAACCAT TCCAGGCCTG GACGGGCTTC TACACGTCCC GCAGCTCACT GAAGGGGCTG 
GCCCGGCGAG CCAGCGCCTT GTTGTATGCC GGGGAGTCCA TGTTCACACG CTACCTGTGG 
CCGGCCCCCC GTGGGCATCT GGACCCCACC TGGGCCCTGC AGCAGCTCCA GCAGCTTCGC 
TGGGCCGTCT CCGAGGTCCA GCACCATGAT GCCATCACTG GGACTGAGTC CCCCAAGGTG 
AGAGACATGT ACGCAACGCA CCTGGCCTCG GGGATGCTGG GCATGCGCAA GCTGATGGCC 
TCCATCGTCC TAGATGAGCT CCAGCCCCAG GCACCCATGG CGGCCAGCTC CGATGCAGGA 
CCTGCAGGAC ATTTTGCCTC GGTCTACAAC CCGCTGGCCT GGACGGTCAC CACCATCGTC 
ACCCTGACTG TTGGTTTCCC TGGAGTCCGC GTCACAGATG AGGCGGGCCA CCCAGTGCCC 
TCGCAGATCC AGAACTCAAC AGAGACCCCA TCTGCGTATG ACCTGCTTAT TCTGACCACA 
ATCCCAGGCC TCAGTTACCG GCACTACAAC ATCAGACCCA CTGCAGGGGC CCAAGAGGGC 
ACCCAGGAGC CGGCTGCCAC TGTGGCGAGC ACCCTTCAAT TTGGCCGCAG GCTGAGGAGA 
CGCACCAGCC ATGCGGGCAG GTACTTGGTG CCTGTGGCAA ACGACTGCTA CATTGTGCTG 
CTCGACCAGG ATACCAACCT GATGCACAGC ATCTGGGAGA GACAGAGTAA CCGAACGGTG 
CGCGTGACCC AGGAATTCCT GGAGTACCAC GTCAACGGGG ATGTGAAACA GGGCCCCATT 
TCCGATAACT ACCTGTTCAC ACCGGGCAAG GCCGCGGTGC CTGCGTGGGA AGCTGTGGAA 
ATGGAGATTG TGGCGGGACA GCTTGTGACT GAGATCCGGC AGTACTTCTA CAGGAACATG 
ACAGCACAGA ATTACACGTA TGCAATCCGC TCCCGGCTCA CCCATGTGCC GCAGGGCCAT 
GACGGGGAGC TGCTCTGCCA CCGGATAGAG CAGGAGTACC AAGCCGGCCC CCTGGAGCTG 
AACCGTGAGG CTGTCCTGAG GACCAGCACC AACCTAAACA GCCAGCAGGT CATCTACTCA 
GACAACAACG GCTACCAGAT GCAGCGGAGG CCCTACGTTT CCTATGTGAA CAACAGCATC 
GCCCGGAATT ACTACCCCAT GGTTCAGTCG GCCTTCATGG AGGATGGCAA AAGCAGGCTT 
GTGTTGCTGT CGGAGCGGGC ACATGGCATC TCCAGCCAAG GGAATGGGCA GGTGGAGGTC 
ATGCTCCACC GGCGGCTGTG GAACAACTTC GACTGGGACC TGGGCTACAA CCTCACGCTG 
AACGACACCT CAGTCGTCCA CCCAGTGCTC TGGCTTCTGC TGGGATCCTG GTCCCTCACC 
ACTGCCCTGC GCCAGAGGAG CGCACTGGCG CTGCAGCACA GGCCCGTGGT GCTGTTCGGA 
GACCTCGCTG GGACTGCGCC GAAGCTCCCA GGACCCCAGC AGCAAGAGGC CGTGACGCTG 
CCCCCGAATC TTCACCTGCA GATCCTGAGC ATCCCTGGCT GGCGCTACAG CTCCAACCAC 
ACGGAGCACT CTCAGAATCT CCGGAAAGGC CATCGAGGGG AAGCCCAGGC TGACCTCCGC 
CGTGTCCTGC TGCGGCTCTA CCACCTATAT GAAGTGGGCG AGGACCCAGT CCTGTCTCAG 
CCAGTGACAG TGAATCTGGA GGCTGTGCTG CAGGCGCTGG GGTCCGTGGT GGCAGTGGAG 
GAGCGCTCGC TCACAGGGAC CTGGGATTTG AGCATGCTGC ACCGCTGGAG CTGGAGGACG 
GGGCCTGGCC GCCACAGAGG TGACACCACC TCTCCCTCGA GGCCACCAGG AGGCCCCATC 
ATCACCGTCC ACCCAAAGGA AATCCGGACG TTCTTTATTC ACTTTCAACA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B2 ( NM_015274.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056089.1 Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108609
Clone ID Related Accession (Same CDS sequence)	NM_015274.3
Accession Version	NM_015274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	epididymis-specific alpha-mannosidase isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093323.3. On May 17, 2019 this sequence version replaced NM_015274.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660803.58648.1, SRR1660805.30291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285599.8/ ENSP00000285599.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGGCCCTGGT GGCCAACGTG AAGCAGAGGG CCGCCTGGTT CCGGACACCG 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ATGCCTCGGT GCAGTTTGCC 
AACATGGACC CGCTGCTGGA CCACATCAAC AGCCATGCTG CCGAGCTCGG TGTCTCGGTG 
CAGTATGCCA CGCTGGGCGA CTACTTCCGT GCCCTGCACG CTCTCAATGT CACCTGGCGT 
GTCCGCGACC ACCACGACTT CCTGCCCTAT TCCACAGAAC CATTCCAGGC CTGGACGGGC 
TTCTACACGT CCCGCAGCTC ACTGAAGGGG CTGGCCCGGC GAGCCAGCGC CTTGTTGTAT 
GCCGGGGAGT CCATGTTCAC ACGCTACCTG TGGCCGGCCC CCCGTGGGCA TCTGGACCCC 
ACCTGGGCCC TGCAGCAGCT CCAGCAGCTT CGCTGGGCCG TCTCCGAGGT CCAGCACCAT 
GATGCCATCA CTGGGACTGA GTCCCCCAAG GTGAGAGACA TGTACGCAAC GCACCTGGCC 
TCGGGGATGC TGGGCATGCG CAAGCTGATG GCCTCCATCG TCCTAGATGA GCTCCAGCCC 
CAGGCACCCA TGGCGGCCAG CTCCGATGCA GGACCTGCAG GACATTTTGC CTCGGTCTAC 
AACCCGCTGG CCTGGACGGT CACCACCATC GTCACCCTGA CTGTTGGTTT CCCTGGAGTC 
CGCGTCACAG ATGAGGCGGG CCACCCAGTG CCCTCGCAGA TCCAGAACTC AACAGAGACC 
CCATCTGCGT ATGACCTGCT TATTCTGACC ACAATCCCAG GCCTCAGTTA CCGGCACTAC 
AACATCAGAC CCACTGCAGG GGCCCAAGAG GGCACCCAGG AGCCGGCTGC CACTGTGGCG 
AGCACCCTTC AATTTGGCCG CAGGCTGAGG AGACGCACCA GCCATGCGGG CAGGTACTTG 
GTGCCTGTGG CAAACGACTG CTACATTGTG CTGCTCGACC AGGATACCAA CCTGATGCAC 
AGCATCTGGG AGAGACAGAG TAACCGAACG GTGCGCGTGA CCCAGGAATT CCTGGAGTAC 
CACGTCAACG GGGATGTGAA ACAGGGCCCC ATTTCCGATA ACTACCTGTT CACACCGGGC 
AAGGCCGCGG TGCCTGCGTG GGAAGCTGTG GAAATGGAGA TTGTGGCGGG ACAGCTTGTG 
ACTGAGATCC GGCAGTACTT CTACAGGAAC ATGACAGCAC AGAATTACAC GTATGCAATC 
CGCTCCCGGC TCACCCATGT GCCGCAGGGC CATGACGGGG AGCTGCTCTG CCACCGGATA 
GAGCAGGAGT ACCAAGCCGG CCCCCTGGAG CTGAACCGTG AGGCTGTCCT GAGGACCAGC 
ACCAACCTAA ACAGCCAGCA GGTCATCTAC TCAGACAACA ACGGCTACCA GATGCAGCGG 
AGGCCCTACG TTTCCTATGT GAACAACAGC ATCGCCCGGA ATTACTACCC CATGGTTCAG 
TCGGCCTTCA TGGAGGATGG CAAAAGCAGG CTTGTGTTGC TGTCGGAGCG GGCACATGGC 
ATCTCCAGCC AAGGGAATGG GCAGGTGGAG GTCATGCTCC ACCGGCGGCT GTGGAACAAC 
TTCGACTGGG ACCTGGGCTA CAACCTCACG CTGAACGACA CCTCAGTCGT CCACCCAGTG 
CTCTGGCTTC TGCTGGGATC CTGGTCCCTC ACCACTGCCC TGCGCCAGAG GAGCGCACTG 
GCGCTGCAGC ACAGGCCCGT GGTGCTGTTC GGAGACCTCG CTGGGACTGC GCCGAAGCTC 
CCAGGACCCC AGCAGCAAGA GGCCGTGACG CTGCCCCCGA ATCTTCACCT GCAGATCCTG 
AGCATCCCTG GCTGGCGCTA CAGCTCCAAC CACACGGAGC ACTCTCAGAA TCTCCGGAAA 
GGCCATCGAG GGGAAGCCCA GGCTGACCTC CGCCGTGTCC TGCTGCGGCT CTACCACCTA 
TATGAAGTGG GCGAGGACCC AGTCCTGTCT CAGCCAGTGA CAGTGAATCT GGAGGCTGTG 
CTGCAGGCGC TGGGGTCCGT GGTGGCAGTG GAGGAGCGCT CGCTCACAGG GACCTGGGAT 
TTGAGCATGC TGCACCGCTG GAGCTGGAGG ACGGGGCCTG GCCGCCACAG AGGTGACACC 
ACCTCTCCCT CGAGGCCACC AGGAGGCCCC ATCATCACCG TCCACCCAAA GGAAATCCGG 
ACGTTCTTTA TTCACTTTCA ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056089.1
CDS	23..3052
Translation	MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPQDGVYPNMSEPVTPANINLYAEALVANVKQRAAWFRTPHVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGMRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYNIRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNGDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ

Target ORF information:

RefSeq Version	NM_015274.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015274.3

ORF Insert Sequence:

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGGCCCTGGT GGCCAACGTG AAGCAGAGGG CCGCCTGGTT CCGGACACCG 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ATGCCTCGGT GCAGTTTGCC 
AACATGGACC CGCTGCTGGA CCACATCAAC AGCCATGCTG CCGAGCTCGG TGTCTCGGTG 
CAGTATGCCA CGCTGGGCGA CTACTTCCGT GCCCTGCACG CTCTCAATGT CACCTGGCGT 
GTCCGCGACC ACCACGACTT CCTGCCCTAT TCCACAGAAC CATTCCAGGC CTGGACGGGC 
TTCTACACGT CCCGCAGCTC ACTGAAGGGG CTGGCCCGGC GAGCCAGCGC CTTGTTGTAT 
GCCGGGGAGT CCATGTTCAC ACGCTACCTG TGGCCGGCCC CCCGTGGGCA TCTGGACCCC 
ACCTGGGCCC TGCAGCAGCT CCAGCAGCTT CGCTGGGCCG TCTCCGAGGT CCAGCACCAT 
GATGCCATCA CTGGGACTGA GTCCCCCAAG GTGAGAGACA TGTACGCAAC GCACCTGGCC 
TCGGGGATGC TGGGCATGCG CAAGCTGATG GCCTCCATCG TCCTAGATGA GCTCCAGCCC 
CAGGCACCCA TGGCGGCCAG CTCCGATGCA GGACCTGCAG GACATTTTGC CTCGGTCTAC 
AACCCGCTGG CCTGGACGGT CACCACCATC GTCACCCTGA CTGTTGGTTT CCCTGGAGTC 
CGCGTCACAG ATGAGGCGGG CCACCCAGTG CCCTCGCAGA TCCAGAACTC AACAGAGACC 
CCATCTGCGT ATGACCTGCT TATTCTGACC ACAATCCCAG GCCTCAGTTA CCGGCACTAC 
AACATCAGAC CCACTGCAGG GGCCCAAGAG GGCACCCAGG AGCCGGCTGC CACTGTGGCG 
AGCACCCTTC AATTTGGCCG CAGGCTGAGG AGACGCACCA GCCATGCGGG CAGGTACTTG 
GTGCCTGTGG CAAACGACTG CTACATTGTG CTGCTCGACC AGGATACCAA CCTGATGCAC 
AGCATCTGGG AGAGACAGAG TAACCGAACG GTGCGCGTGA CCCAGGAATT CCTGGAGTAC 
CACGTCAACG GGGATGTGAA ACAGGGCCCC ATTTCCGATA ACTACCTGTT CACACCGGGC 
AAGGCCGCGG TGCCTGCGTG GGAAGCTGTG GAAATGGAGA TTGTGGCGGG ACAGCTTGTG 
ACTGAGATCC GGCAGTACTT CTACAGGAAC ATGACAGCAC AGAATTACAC GTATGCAATC 
CGCTCCCGGC TCACCCATGT GCCGCAGGGC CATGACGGGG AGCTGCTCTG CCACCGGATA 
GAGCAGGAGT ACCAAGCCGG CCCCCTGGAG CTGAACCGTG AGGCTGTCCT GAGGACCAGC 
ACCAACCTAA ACAGCCAGCA GGTCATCTAC TCAGACAACA ACGGCTACCA GATGCAGCGG 
AGGCCCTACG TTTCCTATGT GAACAACAGC ATCGCCCGGA ATTACTACCC CATGGTTCAG 
TCGGCCTTCA TGGAGGATGG CAAAAGCAGG CTTGTGTTGC TGTCGGAGCG GGCACATGGC 
ATCTCCAGCC AAGGGAATGG GCAGGTGGAG GTCATGCTCC ACCGGCGGCT GTGGAACAAC 
TTCGACTGGG ACCTGGGCTA CAACCTCACG CTGAACGACA CCTCAGTCGT CCACCCAGTG 
CTCTGGCTTC TGCTGGGATC CTGGTCCCTC ACCACTGCCC TGCGCCAGAG GAGCGCACTG 
GCGCTGCAGC ACAGGCCCGT GGTGCTGTTC GGAGACCTCG CTGGGACTGC GCCGAAGCTC 
CCAGGACCCC AGCAGCAAGA GGCCGTGACG CTGCCCCCGA ATCTTCACCT GCAGATCCTG 
AGCATCCCTG GCTGGCGCTA CAGCTCCAAC CACACGGAGC ACTCTCAGAA TCTCCGGAAA 
GGCCATCGAG GGGAAGCCCA GGCTGACCTC CGCCGTGTCC TGCTGCGGCT CTACCACCTA 
TATGAAGTGG GCGAGGACCC AGTCCTGTCT CAGCCAGTGA CAGTGAATCT GGAGGCTGTG 
CTGCAGGCGC TGGGGTCCGT GGTGGCAGTG GAGGAGCGCT CGCTCACAGG GACCTGGGAT 
TTGAGCATGC TGCACCGCTG GAGCTGGAGG ACGGGGCCTG GCCGCCACAG AGGTGACACC 
ACCTCTCCCT CGAGGCCACC AGGAGGCCCC ATCATCACCG TCCACCCAAA GGAAATCCGG 
ACGTTCTTTA TTCACTTTCA ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B2 ( NM_015274.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056089.1 Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24671
Clone ID Related Accession (Same CDS sequence)	NM_015274.2
Accession Version	NM_015274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	epididymis-specific alpha-mannosidase isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE243063.1, BC033307.1, AL833071.2, BM730115.1, BX350248.2 and AK025803.1. On May 17, 2014 this sequence version replaced NM_015274.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC094773.1, SRR1803612.245311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGGCCCTGGT GGCCAACGTG AAGCAGAGGG CCGCCTGGTT CCGGACACCG 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ATGCCTCGGT GCAGTTTGCC 
AACATGGACC CGCTGCTGGA CCACATCAAC AGCCATGCTG CCGAGCTCGG TGTCTCGGTG 
CAGTATGCCA CGCTGGGCGA CTACTTCCGT GCCCTGCACG CTCTCAATGT CACCTGGCGT 
GTCCGCGACC ACCACGACTT CCTGCCCTAT TCCACAGAAC CATTCCAGGC CTGGACGGGC 
TTCTACACGT CCCGCAGCTC ACTGAAGGGG CTGGCCCGGC GAGCCAGCGC CTTGTTGTAT 
GCCGGGGAGT CCATGTTCAC ACGCTACCTG TGGCCGGCCC CCCGTGGGCA TCTGGACCCC 
ACCTGGGCCC TGCAGCAGCT CCAGCAGCTT CGCTGGGCCG TCTCCGAGGT CCAGCACCAT 
GATGCCATCA CTGGGACTGA GTCCCCCAAG GTGAGAGACA TGTACGCAAC GCACCTGGCC 
TCGGGGATGC TGGGCATGCG CAAGCTGATG GCCTCCATCG TCCTAGATGA GCTCCAGCCC 
CAGGCACCCA TGGCGGCCAG CTCCGATGCA GGACCTGCAG GACATTTTGC CTCGGTCTAC 
AACCCGCTGG CCTGGACGGT CACCACCATC GTCACCCTGA CTGTTGGTTT CCCTGGAGTC 
CGCGTCACAG ATGAGGCGGG CCACCCAGTG CCCTCGCAGA TCCAGAACTC AACAGAGACC 
CCATCTGCGT ATGACCTGCT TATTCTGACC ACAATCCCAG GCCTCAGTTA CCGGCACTAC 
AACATCAGAC CCACTGCAGG GGCCCAAGAG GGCACCCAGG AGCCGGCTGC CACTGTGGCG 
AGCACCCTTC AATTTGGCCG CAGGCTGAGG AGACGCACCA GCCATGCGGG CAGGTACTTG 
GTGCCTGTGG CAAACGACTG CTACATTGTG CTGCTCGACC AGGATACCAA CCTGATGCAC 
AGCATCTGGG AGAGACAGAG TAACCGAACG GTGCGCGTGA CCCAGGAATT CCTGGAGTAC 
CACGTCAACG GGGATGTGAA ACAGGGCCCC ATTTCCGATA ACTACCTGTT CACACCGGGC 
AAGGCCGCGG TGCCTGCGTG GGAAGCTGTG GAAATGGAGA TTGTGGCGGG ACAGCTTGTG 
ACTGAGATCC GGCAGTACTT CTACAGGAAC ATGACAGCAC AGAATTACAC GTATGCAATC 
CGCTCCCGGC TCACCCATGT GCCGCAGGGC CATGACGGGG AGCTGCTCTG CCACCGGATA 
GAGCAGGAGT ACCAAGCCGG CCCCCTGGAG CTGAACCGTG AGGCTGTCCT GAGGACCAGC 
ACCAACCTAA ACAGCCAGCA GGTCATCTAC TCAGACAACA ACGGCTACCA GATGCAGCGG 
AGGCCCTACG TTTCCTATGT GAACAACAGC ATCGCCCGGA ATTACTACCC CATGGTTCAG 
TCGGCCTTCA TGGAGGATGG CAAAAGCAGG CTTGTGTTGC TGTCGGAGCG GGCACATGGC 
ATCTCCAGCC AAGGGAATGG GCAGGTGGAG GTCATGCTCC ACCGGCGGCT GTGGAACAAC 
TTCGACTGGG ACCTGGGCTA CAACCTCACG CTGAACGACA CCTCAGTCGT CCACCCAGTG 
CTCTGGCTTC TGCTGGGATC CTGGTCCCTC ACCACTGCCC TGCGCCAGAG GAGCGCACTG 
GCGCTGCAGC ACAGGCCCGT GGTGCTGTTC GGAGACCTCG CTGGGACTGC GCCGAAGCTC 
CCAGGACCCC AGCAGCAAGA GGCCGTGACG CTGCCCCCGA ATCTTCACCT GCAGATCCTG 
AGCATCCCTG GCTGGCGCTA CAGCTCCAAC CACACGGAGC ACTCTCAGAA TCTCCGGAAA 
GGCCATCGAG GGGAAGCCCA GGCTGACCTC CGCCGTGTCC TGCTGCGGCT CTACCACCTG 
TATGAAGTGG GCGAGGACCC AGTCCTGTCT CAGCCAGTGA CAGTGAATCT GGAGGCTGTG 
CTGCAGGCGC TGGGGTCCGT GGTGGCAGTG GAGGAGCGCT CGCTCACAGG GACCTGGGAT 
TTGAGCATGC TGCACCGCTG GAGCTGGAGG ACGGGGCCTG GCCGCCACAG AGGTGACACC 
ACCTCTCCCT CGAGGCCACC AGGAGGCCCC ATCATCACCG TCCACCCAAA GGAAATCCGG 
ACGTTCTTTA TTCACTTTCA ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056089.1
CDS	38..3067
Translation	MGQLCWLPLLAPLLLLRPPGVQSAGPIRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELARGQQRRFIAVEQEFFRLWWDGVASDQQKYQVRQLLEEGRLEFVIGGQVMHDEAVTHLDDQILQLTEGHGFLYETFGIRPQFSWHVDPFGASATTPTLFALAGFNAHLGSRIDYDLKAAMQEARGLQFVWRGSPSLSERQEIFTHIMDQYSYCTPSHIPFSNRSGFYWNGVAVFPKPPQDGVYPNMSEPVTPANINLYAEALVANVKQRAAWFRTPHVLWPWGCDKQFFNASVQFANMDPLLDHINSHAAELGVSVQYATLGDYFRALHALNVTWRVRDHHDFLPYSTEPFQAWTGFYTSRSSLKGLARRASALLYAGESMFTRYLWPAPRGHLDPTWALQQLQQLRWAVSEVQHHDAITGTESPKVRDMYATHLASGMLGMRKLMASIVLDELQPQAPMAASSDAGPAGHFASVYNPLAWTVTTIVTLTVGFPGVRVTDEAGHPVPSQIQNSTETPSAYDLLILTTIPGLSYRHYNIRPTAGAQEGTQEPAATVASTLQFGRRLRRRTSHAGRYLVPVANDCYIVLLDQDTNLMHSIWERQSNRTVRVTQEFLEYHVNGDVKQGPISDNYLFTPGKAAVPAWEAVEMEIVAGQLVTEIRQYFYRNMTAQNYTYAIRSRLTHVPQGHDGELLCHRIEQEYQAGPLELNREAVLRTSTNLNSQQVIYSDNNGYQMQRRPYVSYVNNSIARNYYPMVQSAFMEDGKSRLVLLSERAHGISSQGNGQVEVMLHRRLWNNFDWDLGYNLTLNDTSVVHPVLWLLLGSWSLTTALRQRSALALQHRPVVLFGDLAGTAPKLPGPQQQEAVTLPPNLHLQILSIPGWRYSSNHTEHSQNLRKGHRGEAQADLRRVLLRLYHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGPGRHRGDTTSPSRPPGGPIITVHPKEIRTFFIHFQQQ

Target ORF information:

RefSeq Version	NM_015274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 2 (MAN2B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015274.2

ORF Insert Sequence:

ATGGGGCAGC TGTGCTGGCT GCCGCTGCTG GCACCGCTCC TGTTGCTGCG ACCGCCAGGG 
GTCCAGTCCG CCGGCCCCAT CCGGGCCTTC GTGGTGCCCC ACAGCCACAT GGACGTGGGC 
TGGGTCTACA CTGTGCAGGA AAGCATGCGG GCGTACGCCG CCAATGTCTA CACCTCAGTG 
GTGGAAGAGC TGGCCCGCGG CCAGCAGCGC CGGTTCATCG CTGTGGAGCA GGAGTTTTTC 
CGGCTGTGGT GGGATGGCGT CGCCTCGGAC CAGCAGAAAT ACCAGGTCCG CCAGCTCCTG 
GAGGAAGGAC GCCTGGAATT TGTCATCGGA GGCCAGGTCA TGCATGACGA GGCTGTGACG 
CACCTTGATG ACCAGATCCT GCAGCTCACA GAAGGACACG GGTTTCTCTA TGAAACATTT 
GGGATCCGGC CACAGTTCTC CTGGCACGTT GACCCGTTTG GCGCCTCTGC CACGACGCCC 
ACCCTATTTG CGCTGGCGGG CTTCAATGCC CACCTCGGCT CCCGGATCGA CTACGACCTG 
AAGGCAGCCA TGCAGGAGGC CCGGGGGCTG CAGTTCGTGT GGCGAGGGTC CCCATCCCTC 
TCAGAGCGGC AGGAAATCTT CACGCACATC ATGGACCAGT ACAGCTACTG CACCCCGTCC 
CACATCCCTT TCTCCAACAG GTCAGGATTT TACTGGAATG GCGTGGCTGT CTTCCCCAAG 
CCTCCCCAAG ATGGGGTGTA CCCCAACATG AGTGAGCCTG TCACCCCAGC CAACATCAAC 
CTCTATGCCG AGGCCCTGGT GGCCAACGTG AAGCAGAGGG CCGCCTGGTT CCGGACACCG 
CACGTCCTCT GGCCCTGGGG ATGTGACAAG CAGTTCTTCA ATGCCTCGGT GCAGTTTGCC 
AACATGGACC CGCTGCTGGA CCACATCAAC AGCCATGCTG CCGAGCTCGG TGTCTCGGTG 
CAGTATGCCA CGCTGGGCGA CTACTTCCGT GCCCTGCACG CTCTCAATGT CACCTGGCGT 
GTCCGCGACC ACCACGACTT CCTGCCCTAT TCCACAGAAC CATTCCAGGC CTGGACGGGC 
TTCTACACGT CCCGCAGCTC ACTGAAGGGG CTGGCCCGGC GAGCCAGCGC CTTGTTGTAT 
GCCGGGGAGT CCATGTTCAC ACGCTACCTG TGGCCGGCCC CCCGTGGGCA TCTGGACCCC 
ACCTGGGCCC TGCAGCAGCT CCAGCAGCTT CGCTGGGCCG TCTCCGAGGT CCAGCACCAT 
GATGCCATCA CTGGGACTGA GTCCCCCAAG GTGAGAGACA TGTACGCAAC GCACCTGGCC 
TCGGGGATGC TGGGCATGCG CAAGCTGATG GCCTCCATCG TCCTAGATGA GCTCCAGCCC 
CAGGCACCCA TGGCGGCCAG CTCCGATGCA GGACCTGCAG GACATTTTGC CTCGGTCTAC 
AACCCGCTGG CCTGGACGGT CACCACCATC GTCACCCTGA CTGTTGGTTT CCCTGGAGTC 
CGCGTCACAG ATGAGGCGGG CCACCCAGTG CCCTCGCAGA TCCAGAACTC AACAGAGACC 
CCATCTGCGT ATGACCTGCT TATTCTGACC ACAATCCCAG GCCTCAGTTA CCGGCACTAC 
AACATCAGAC CCACTGCAGG GGCCCAAGAG GGCACCCAGG AGCCGGCTGC CACTGTGGCG 
AGCACCCTTC AATTTGGCCG CAGGCTGAGG AGACGCACCA GCCATGCGGG CAGGTACTTG 
GTGCCTGTGG CAAACGACTG CTACATTGTG CTGCTCGACC AGGATACCAA CCTGATGCAC 
AGCATCTGGG AGAGACAGAG TAACCGAACG GTGCGCGTGA CCCAGGAATT CCTGGAGTAC 
CACGTCAACG GGGATGTGAA ACAGGGCCCC ATTTCCGATA ACTACCTGTT CACACCGGGC 
AAGGCCGCGG TGCCTGCGTG GGAAGCTGTG GAAATGGAGA TTGTGGCGGG ACAGCTTGTG 
ACTGAGATCC GGCAGTACTT CTACAGGAAC ATGACAGCAC AGAATTACAC GTATGCAATC 
CGCTCCCGGC TCACCCATGT GCCGCAGGGC CATGACGGGG AGCTGCTCTG CCACCGGATA 
GAGCAGGAGT ACCAAGCCGG CCCCCTGGAG CTGAACCGTG AGGCTGTCCT GAGGACCAGC 
ACCAACCTAA ACAGCCAGCA GGTCATCTAC TCAGACAACA ACGGCTACCA GATGCAGCGG 
AGGCCCTACG TTTCCTATGT GAACAACAGC ATCGCCCGGA ATTACTACCC CATGGTTCAG 
TCGGCCTTCA TGGAGGATGG CAAAAGCAGG CTTGTGTTGC TGTCGGAGCG GGCACATGGC 
ATCTCCAGCC AAGGGAATGG GCAGGTGGAG GTCATGCTCC ACCGGCGGCT GTGGAACAAC 
TTCGACTGGG ACCTGGGCTA CAACCTCACG CTGAACGACA CCTCAGTCGT CCACCCAGTG 
CTCTGGCTTC TGCTGGGATC CTGGTCCCTC ACCACTGCCC TGCGCCAGAG GAGCGCACTG 
GCGCTGCAGC ACAGGCCCGT GGTGCTGTTC GGAGACCTCG CTGGGACTGC GCCGAAGCTC 
CCAGGACCCC AGCAGCAAGA GGCCGTGACG CTGCCCCCGA ATCTTCACCT GCAGATCCTG 
AGCATCCCTG GCTGGCGCTA CAGCTCCAAC CACACGGAGC ACTCTCAGAA TCTCCGGAAA 
GGCCATCGAG GGGAAGCCCA GGCTGACCTC CGCCGTGTCC TGCTGCGGCT CTACCACCTG 
TATGAAGTGG GCGAGGACCC AGTCCTGTCT CAGCCAGTGA CAGTGAATCT GGAGGCTGTG 
CTGCAGGCGC TGGGGTCCGT GGTGGCAGTG GAGGAGCGCT CGCTCACAGG GACCTGGGAT 
TTGAGCATGC TGCACCGCTG GAGCTGGAGG ACGGGGCCTG GCCGCCACAG AGGTGACACC 
ACCTCTCCCT CGAGGCCACC AGGAGGCCCC ATCATCACCG TCCACCCAAA GGAAATCCGG 
ACGTTCTTTA TTCACTTTCA ACAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Mutation research694(1-2)13-9(2010 Dec)
Flachsbart F,Franke A,Kleindorp R,Caliebe A,Blanch? H,Schreiber S,Nebel A

Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties.
Protein science : a publication of the Protein Society18(11)2242-51(2009 Nov)
Venkatesan M,Kuntz DA,Rose DR

Characterization of a human core-specific lysosomal {alpha}1,6-mannosidase involved in N-glycan catabolism.
The Journal of biological chemistry280(44)37204-16(2005 Nov)
Park C,Meng L,Stanton LH,Collins RE,Mast SW,Yi X,Strachan H,Moremen KW

Purification and properties of major alpha-D-mannosidase in the luminal fluid of porcine epididymis.
Biochimica et biophysica acta1432(2)382-92(1999 Jul)
Jin YZ,Dacheux F,Dacheux JL,Bannai S,Sugita Y,Okamura N

Stage-specific expression of a mouse homologue of the porcine 135kDa alpha-D-mannosidase (MAN2B2) in type A spermatogonia.
Biochemical and biophysical research communications241(2)439-45(1997 Dec)
Hiramoto S,Tamba M,Kiuchi S,Jin YZ,Bannai S,Sugita Y,Dacheux F,Dacheux JL,Yoshida M,Okamura N

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MAN2B2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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