DENND5A cDNA ORF clone, Homo sapiens(human)

The following DENND5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu65820 XM_011519953.2
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu92068 XM_017017419.1
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu100922 NM_001348750.2
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu100924 NM_001348749.2
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu02229 NM_001243254.2
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu06879 NM_015213.4
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu06879 NM_001348748.1
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu44799 XM_005252832.1
Latest version!
Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu02229 NM_001243254.1 Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu100924 NM_001348749.1 Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu100922 NM_001348750.1 Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu06879 NM_015213.3 Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu65820
    Clone ID Related Accession (Same CDS sequence) XM_011519953.2
    Accession Version XM_011519953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2022bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGAAAG CAATTGAGCT GCGTCTGGCA AAAATTGACC ATACTGCAAT TCACCCACAT 
    TTACTTGACA TGAAGATTGG ACAAGGGAAA TATGAGCCGG GCTTCTTCCC TAAGCTGCAG
    TCTGATGTAC TTTCCACTGG GCCAGCCAGC AACAAGTGGA CGAAAAGGAA TGCCCCTGCC
    CAGTGGAGGC GGAAAGATCG GCAGAAGCAG CACACAGAAC ACCTGCGTTT AGATAATGAC
    CAGAGGGAGA AGTACATCCA GGAAGCCAGG ACTATGGGCA GCACTATCCG CCAGCCCAAA
    CTGTCCAACC TCTCTCCATC AGTGATTGCC CAGACCAATT GGAAGTTTGT AGAGGGCCTG
    CTGAAGGAAT GCCGCAATAA GACCAAGAGG ATGCTGGTGG AAAAGATGGG CCGAGAAGCT
    GTGGAGCTAG GGCATGGGGA GGTGAACATC ACAGGGGTGG AAGAGAACAC CCTGATTGCC
    AGCCTTTGTG ATCTCCTGGA AAGGATCTGG AGTCATGGAC TACAAGTGAA ACAGGGGAAA
    TCAGCCTTAT GGTCCCACCT GTTACATTAT CAGGACAACC GGCAGAGAAA ACTCACATCA
    GGAAGCCTCA GTACCTCAGG AATACTTCTT GATTCAGAAC GTAGGAAGTC TGATGCCAGC
    TCACTCATGC CTCCCCTGAG GATCTCCCTG ATTCAGGATA TGAGGCACAT CCAGAACATC
    GGGGAAATCA AGACTGATGT GGGAAAGGCC AGAGCATGGG TGCGACTGTC CATGGAAAAA
    AAGTTACTTT CCAGACACCT GAAGCAGCTC CTCTCAGACC ATGAGCTCAC CAAAAAGTTA
    TATAAGCGCT ATGCCTTCCT GCGCTGTGAT GACGAGAAGG AGCAGTTCCT CTATCACCTC
    CTGTCTTTCA ATGCCGTCGA TTACTTTTGC TTCACCAATG TCTTCACAAC TATCCTGATC
    CCGTACCACA TTCTGATCGT ACCAAGCAAG AAGCTGGGGG GCTCCATGTT CACTGCCAAC
    CCATGGATCT GTATATCAGG AGAATTGGGT GAGACACAGA TCATGCAGAT TCCCAGGAAT
    GTGCTAGAGA TGACCTTCGA GTGCCAGAAC TTGGGGAAGC TTACTACTGT CCAGATTGGC
    CATGATAACT CTGGGCTGTA TGCCAAATGG CTGGTGGAGT ATGTGATGGT CAGGAATGAG
    ATCACAGGAC ATACCTACAA GTTCCCGTGT GGCCGGTGGT TAGGGAAGGG CATGGATGAT
    GGAAGCCTGG AGCGGATCCT AGTTGGGGAG CTGCTCACAT CCCAGCCTGA GGTGGATGAG
    AGGCCATGCC GGACCCCGCC GCTGCAGCAG TCCCCCAGTG TCATCCGGAG GCTTGTTACC
    ATCTCACCCA ACAACAAGCC CAAGCTGAAC ACTGGGCAGA TCCAGGAGTC CATCGGGGAG
    GCAGTCAATG GCATTGTGAA GCACTTCCAT AAGCCTGAGA AAGAGCGAGG CAGTCTGACG
    CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC TCGGCCTTGG AACAGGCTTT CCAGCATGGA
    TTTAAATCGC CCCGGCTCTT CAAAAATGTC TTCATTTGGG ATTTCCTGGA AAAAGCACAA
    ACCTATTATG AGACATTAGA GAAGAATGAA GTAGTCCCTG AGGAAAACTG GCATACAAGA
    GCCCGGAACT TCTGCCGATT TGTCACTGCA ATCAACAATA CTCCCCGGAA CATCGGCAAG
    GATGGCAAGT TTCAGATGCT GGTGTGCTTG GGAGCCAGGG TTATCATGAA GATTAAATCT
    CTTATGTCAG TGCCAGCACA TGCTGAGTGT TATGTGAGAG ATCACCTCCT ACACCACTGG
    ATTGCCCTGC TGGCTGACTG CCCCATCACT GCACACATGT ATGAGGATGT GGCACTGATC
    AAAGACCATA CACTTGTCAA TTCCTTGATT CGTGTGCTGC AGACATTGCA GGAGTTCAAC
    ATCACGCTGG AGACGTCCCT TGTCAAGGGC ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518255.1
    CDS423..2444
    Translation

    Target ORF information:

    RefSeq Version XM_011519953.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGAAAG CAATTGAGCT GCGTCTGGCA AAAATTGACC ATACTGCAAT TCACCCACAT 
    TTACTTGACA TGAAGATTGG ACAAGGGAAA TATGAGCCGG GCTTCTTCCC TAAGCTGCAG
    TCTGATGTAC TTTCCACTGG GCCAGCCAGC AACAAGTGGA CGAAAAGGAA TGCCCCTGCC
    CAGTGGAGGC GGAAAGATCG GCAGAAGCAG CACACAGAAC ACCTGCGTTT AGATAATGAC
    CAGAGGGAGA AGTACATCCA GGAAGCCAGG ACTATGGGCA GCACTATCCG CCAGCCCAAA
    CTGTCCAACC TCTCTCCATC AGTGATTGCC CAGACCAATT GGAAGTTTGT AGAGGGCCTG
    CTGAAGGAAT GCCGCAATAA GACCAAGAGG ATGCTGGTGG AAAAGATGGG CCGAGAAGCT
    GTGGAGCTAG GGCATGGGGA GGTGAACATC ACAGGGGTGG AAGAGAACAC CCTGATTGCC
    AGCCTTTGTG ATCTCCTGGA AAGGATCTGG AGTCATGGAC TACAAGTGAA ACAGGGGAAA
    TCAGCCTTAT GGTCCCACCT GTTACATTAT CAGGACAACC GGCAGAGAAA ACTCACATCA
    GGAAGCCTCA GTACCTCAGG AATACTTCTT GATTCAGAAC GTAGGAAGTC TGATGCCAGC
    TCACTCATGC CTCCCCTGAG GATCTCCCTG ATTCAGGATA TGAGGCACAT CCAGAACATC
    GGGGAAATCA AGACTGATGT GGGAAAGGCC AGAGCATGGG TGCGACTGTC CATGGAAAAA
    AAGTTACTTT CCAGACACCT GAAGCAGCTC CTCTCAGACC ATGAGCTCAC CAAAAAGTTA
    TATAAGCGCT ATGCCTTCCT GCGCTGTGAT GACGAGAAGG AGCAGTTCCT CTATCACCTC
    CTGTCTTTCA ATGCCGTCGA TTACTTTTGC TTCACCAATG TCTTCACAAC TATCCTGATC
    CCGTACCACA TTCTGATCGT ACCAAGCAAG AAGCTGGGGG GCTCCATGTT CACTGCCAAC
    CCATGGATCT GTATATCAGG AGAATTGGGT GAGACACAGA TCATGCAGAT TCCCAGGAAT
    GTGCTAGAGA TGACCTTCGA GTGCCAGAAC TTGGGGAAGC TTACTACTGT CCAGATTGGC
    CATGATAACT CTGGGCTGTA TGCCAAATGG CTGGTGGAGT ATGTGATGGT CAGGAATGAG
    ATCACAGGAC ATACCTACAA GTTCCCGTGT GGCCGGTGGT TAGGGAAGGG CATGGATGAT
    GGAAGCCTGG AGCGGATCCT AGTTGGGGAG CTGCTCACAT CCCAGCCTGA GGTGGATGAG
    AGGCCATGCC GGACCCCGCC GCTGCAGCAG TCCCCCAGTG TCATCCGGAG GCTTGTTACC
    ATCTCACCCA ACAACAAGCC CAAGCTGAAC ACTGGGCAGA TCCAGGAGTC CATCGGGGAG
    GCAGTCAATG GCATTGTGAA GCACTTCCAT AAGCCTGAGA AAGAGCGAGG CAGTCTGACG
    CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC TCGGCCTTGG AACAGGCTTT CCAGCATGGA
    TTTAAATCGC CCCGGCTCTT CAAAAATGTC TTCATTTGGG ATTTCCTGGA AAAAGCACAA
    ACCTATTATG AGACATTAGA GAAGAATGAA GTAGTCCCTG AGGAAAACTG GCATACAAGA
    GCCCGGAACT TCTGCCGATT TGTCACTGCA ATCAACAATA CTCCCCGGAA CATCGGCAAG
    GATGGCAAGT TTCAGATGCT GGTGTGCTTG GGAGCCAGGG TTATCATGAA GATTAAATCT
    CTTATGTCAG TGCCAGCACA TGCTGAGTGT TATGTGAGAG ATCACCTCCT ACACCACTGG
    ATTGCCCTGC TGGCTGACTG CCCCATCACT GCACACATGT ATGAGGATGT GGCACTGATC
    AAAGACCATA CACTTGTCAA TTCCTTGATT CGTGTGCTGC AGACATTGCA GGAGTTCAAC
    ATCACGCTGG AGACGTCCCT TGTCAAGGGC ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92068
    Clone ID Related Accession (Same CDS sequence) XM_017017419.1
    Accession Version XM_017017419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGTCTG TATATGTGTT
    CGTCTACTTC TGGATTCTCT GTTCTGTTTC ATTGATCTGT TGGTCTACAT TTATACCAAT
    ACCACACTGT CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872908.1
    CDS267..2261
    Translation

    Target ORF information:

    RefSeq Version XM_017017419.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGTCTG TATATGTGTT
    CGTCTACTTC TGGATTCTCT GTTCTGTTTC ATTGATCTGT TGGTCTACAT TTATACCAAT
    ACCACACTGT CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100922
    Clone ID Related Accession (Same CDS sequence) NM_001348750.1 , NM_001348750.2
    Accession Version NM_001348750.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, AP006259.1 and AC079296.5. Transcript Variant: This variant (4) has an additional alternate exon in its 5' end which results in the use of a downstream in-frame start codon, compared to variant 1, and encodes a shorter isoform (4), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.267992.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGTA TGCCGAAAGG GCTGGCATTC AAGACCCAGG CTGATCCCAG GGAGCCCCAA 
    TTCCATGCCT TTATTATCAC AAGGGAGGAT GGCTCTCGGA CATTTGGGTT TGCCCTCACA
    TTTTATGAAG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CACAATGCTG AGTATGATGT CCTACATGCT CCCCCTGCTG ATGACAGAGA CCAGAGCAGC
    ATGGAGGATG GTGAAGACAC TCCTGTGACC AAACTGCAGC GCTTCAACTC CTATGACATT
    AGCCGGGACA CTCTCTACGT CTCTAAGTGC ATCTGCCTCA TCACACCCAT GTCTTTCATG
    AAGGCATGTC GGAGCGTGCT GGAGCAACTC CACCAGGCAG TCACTTCACC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAC GTACTCTACG AGGTGCCGCT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGGGTCTAT GGGCCAATAA TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTATTTGA CTTTCCTGTC AAAGAGGTTT TTGAACTGCT CGGGGTGGAG
    AATGTGTTTC AGCTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGA GACTGATGAC TGTGGCGGAG ACGATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTCCC TATTCTCCCA GCTTCTCTCC TGCATTTCTT AGATGCTCCT
    GTTCCATACC TGATGGGTTT GCATTCCAAT GGCCTGGATG ACCGGTCAAA GCTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTGCCAGAG
    GACTTGCCAC AGTTCCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGAA TTCCCCCTGA AGGGAATCTT CATTGCAGTG AGAGTGCCTC CAAGCTGAAG
    AGGCTGCGGG CCTCTGAGCT TGTCTCGGAC AAGAGGAATG GGAACATTGC TGGCTCCCCT
    TTGCATTCCT ACGAGCTTCT TAAGGAGAAT GAAACTATTG CCCGGCTGCA AGCCTTGGTC
    AAGAGAACTG GGGTGAGCCT GGAAAAGTTG GAAGTGCGTG AAGACCCCAG CAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATC
    CAACCCAGCC AGGATAAGGA ATCCTGGTTT ACCAACAGGG AGCAAATGCA AAACTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAG CCCTACCTGC CCTTCCTCTC AAGATTCCTG
    GAGACCCAGA TGTTTGCATC TTTCATTGAC AACAAAATAA TGTGTCATGA TGATGATGAT
    AAAGACCCTG TACTCCGGGT ATTTGATTCC CGAGTTGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTCCGTAC ATCCATGTAC CAGAAGTGTA CCACTGTGGA TGAAGCAGAG
    AAAGCAATTG AGCTGCGTCT GGCAAAAATT GACCATACTG CAATTCACCC ACATTTACTT
    GACATGAAGA TTGGACAAGG GAAATATGAG CCGGGCTTCT TCCCTAAGCT GCAGTCTGAT
    GTACTTTCCA CTGGGCCAGC CAGCAACAAG TGGACGAAAA GGAATGCCCC TGCCCAGTGG
    AGGCGGAAAG ATCGGCAGAA GCAGCACACA GAACACCTGC GTTTAGATAA TGACCAGAGG
    GAGAAGTACA TCCAGGAAGC CAGGACTATG GGCAGCACTA TCCGCCAGCC CAAACTGTCC
    AACCTCTCTC CATCAGTGAT TGCCCAGACC AATTGGAAGT TTGTAGAGGG CCTGCTGAAG
    GAATGCCGCA ATAAGACCAA GAGGATGCTG GTGGAAAAGA TGGGCCGAGA AGCTGTGGAG
    CTAGGGCATG GGGAGGTGAA CATCACAGGG GTGGAAGAGA ACACCCTGAT TGCCAGCCTT
    TGTGATCTCC TGGAAAGGAT CTGGAGTCAT GGACTACAAG TGAAACAGGG GAAATCAGCC
    TTATGGTCCC ACCTGTTACA TTATCAGGAC AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATACT TCTTGATTCA GAACGTAGGA AGTCTGATGC CAGCTCACTC
    ATGCCTCCCC TGAGGATCTC CCTGATTCAG GATATGAGGC ACATCCAGAA CATCGGGGAA
    ATCAAGACTG ATGTGGGAAA GGCCAGAGCA TGGGTGCGAC TGTCCATGGA AAAAAAGTTA
    CTTTCCAGAC ACCTGAAGCA GCTCCTCTCA GACCATGAGC TCACCAAAAA GTTATATAAG
    CGCTATGCCT TCCTGCGCTG TGATGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTGTCT
    TTCAATGCCG TCGATTACTT TTGCTTCACC AATGTCTTCA CAACTATCCT GATCCCGTAC
    CACATTCTGA TCGTACCAAG CAAGAAGCTG GGGGGCTCCA TGTTCACTGC CAACCCATGG
    ATCTGTATAT CAGGAGAATT GGGTGAGACA CAGATCATGC AGATTCCCAG GAATGTGCTA
    GAGATGACCT TCGAGTGCCA GAACTTGGGG AAGCTTACTA CTGTCCAGAT TGGCCATGAT
    AACTCTGGGC TGTATGCCAA ATGGCTGGTG GAGTATGTGA TGGTCAGGAA TGAGATCACA
    GGACATACCT ACAAGTTCCC GTGTGGCCGG TGGTTAGGGA AGGGCATGGA TGATGGAAGC
    CTGGAGCGGA TCCTAGTTGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCA
    TGCCGGACCC CGCCGCTGCA GCAGTCCCCC AGTGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA AGCCCAAGCT GAACACTGGG CAGATCCAGG AGTCCATCGG GGAGGCAGTC
    AATGGCATTG TGAAGCACTT CCATAAGCCT GAGAAAGAGC GAGGCAGTCT GACGCTGTTG
    CTCTGTGGAG AGTGTGGCCT TGTCTCGGCC TTGGAACAGG CTTTCCAGCA TGGATTTAAA
    TCGCCCCGGC TCTTCAAAAA TGTCTTCATT TGGGATTTCC TGGAAAAAGC ACAAACCTAT
    TATGAGACAT TAGAGAAGAA TGAAGTAGTC CCTGAGGAAA ACTGGCATAC AAGAGCCCGG
    AACTTCTGCC GATTTGTCAC TGCAATCAAC AATACTCCCC GGAACATCGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGATCACC TCCTACACCA CTGGATTGCC
    CTGCTGGCTG ACTGCCCCAT CACTGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CATACACTTG TCAATTCCTT GATTCGTGTG CTGCAGACAT TGCAGGAGTT CAACATCACG
    CTGGAGACGT CCCTTGTCAA GGGCATCGAC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335679.1
    CDS582..4157
    Translation

    Target ORF information:

    RefSeq Version NM_001348750.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGTA TGCCGAAAGG GCTGGCATTC AAGACCCAGG CTGATCCCAG GGAGCCCCAA 
    TTCCATGCCT TTATTATCAC AAGGGAGGAT GGCTCTCGGA CATTTGGGTT TGCCCTCACA
    TTTTATGAAG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CACAATGCTG AGTATGATGT CCTACATGCT CCCCCTGCTG ATGACAGAGA CCAGAGCAGC
    ATGGAGGATG GTGAAGACAC TCCTGTGACC AAACTGCAGC GCTTCAACTC CTATGACATT
    AGCCGGGACA CTCTCTACGT CTCTAAGTGC ATCTGCCTCA TCACACCCAT GTCTTTCATG
    AAGGCATGTC GGAGCGTGCT GGAGCAACTC CACCAGGCAG TCACTTCACC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAC GTACTCTACG AGGTGCCGCT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGGGTCTAT GGGCCAATAA TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTATTTGA CTTTCCTGTC AAAGAGGTTT TTGAACTGCT CGGGGTGGAG
    AATGTGTTTC AGCTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGA GACTGATGAC TGTGGCGGAG ACGATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTCCC TATTCTCCCA GCTTCTCTCC TGCATTTCTT AGATGCTCCT
    GTTCCATACC TGATGGGTTT GCATTCCAAT GGCCTGGATG ACCGGTCAAA GCTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTGCCAGAG
    GACTTGCCAC AGTTCCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGAA TTCCCCCTGA AGGGAATCTT CATTGCAGTG AGAGTGCCTC CAAGCTGAAG
    AGGCTGCGGG CCTCTGAGCT TGTCTCGGAC AAGAGGAATG GGAACATTGC TGGCTCCCCT
    TTGCATTCCT ACGAGCTTCT TAAGGAGAAT GAAACTATTG CCCGGCTGCA AGCCTTGGTC
    AAGAGAACTG GGGTGAGCCT GGAAAAGTTG GAAGTGCGTG AAGACCCCAG CAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATC
    CAACCCAGCC AGGATAAGGA ATCCTGGTTT ACCAACAGGG AGCAAATGCA AAACTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAG CCCTACCTGC CCTTCCTCTC AAGATTCCTG
    GAGACCCAGA TGTTTGCATC TTTCATTGAC AACAAAATAA TGTGTCATGA TGATGATGAT
    AAAGACCCTG TACTCCGGGT ATTTGATTCC CGAGTTGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTCCGTAC ATCCATGTAC CAGAAGTGTA CCACTGTGGA TGAAGCAGAG
    AAAGCAATTG AGCTGCGTCT GGCAAAAATT GACCATACTG CAATTCACCC ACATTTACTT
    GACATGAAGA TTGGACAAGG GAAATATGAG CCGGGCTTCT TCCCTAAGCT GCAGTCTGAT
    GTACTTTCCA CTGGGCCAGC CAGCAACAAG TGGACGAAAA GGAATGCCCC TGCCCAGTGG
    AGGCGGAAAG ATCGGCAGAA GCAGCACACA GAACACCTGC GTTTAGATAA TGACCAGAGG
    GAGAAGTACA TCCAGGAAGC CAGGACTATG GGCAGCACTA TCCGCCAGCC CAAACTGTCC
    AACCTCTCTC CATCAGTGAT TGCCCAGACC AATTGGAAGT TTGTAGAGGG CCTGCTGAAG
    GAATGCCGCA ATAAGACCAA GAGGATGCTG GTGGAAAAGA TGGGCCGAGA AGCTGTGGAG
    CTAGGGCATG GGGAGGTGAA CATCACAGGG GTGGAAGAGA ACACCCTGAT TGCCAGCCTT
    TGTGATCTCC TGGAAAGGAT CTGGAGTCAT GGACTACAAG TGAAACAGGG GAAATCAGCC
    TTATGGTCCC ACCTGTTACA TTATCAGGAC AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATACT TCTTGATTCA GAACGTAGGA AGTCTGATGC CAGCTCACTC
    ATGCCTCCCC TGAGGATCTC CCTGATTCAG GATATGAGGC ACATCCAGAA CATCGGGGAA
    ATCAAGACTG ATGTGGGAAA GGCCAGAGCA TGGGTGCGAC TGTCCATGGA AAAAAAGTTA
    CTTTCCAGAC ACCTGAAGCA GCTCCTCTCA GACCATGAGC TCACCAAAAA GTTATATAAG
    CGCTATGCCT TCCTGCGCTG TGATGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTGTCT
    TTCAATGCCG TCGATTACTT TTGCTTCACC AATGTCTTCA CAACTATCCT GATCCCGTAC
    CACATTCTGA TCGTACCAAG CAAGAAGCTG GGGGGCTCCA TGTTCACTGC CAACCCATGG
    ATCTGTATAT CAGGAGAATT GGGTGAGACA CAGATCATGC AGATTCCCAG GAATGTGCTA
    GAGATGACCT TCGAGTGCCA GAACTTGGGG AAGCTTACTA CTGTCCAGAT TGGCCATGAT
    AACTCTGGGC TGTATGCCAA ATGGCTGGTG GAGTATGTGA TGGTCAGGAA TGAGATCACA
    GGACATACCT ACAAGTTCCC GTGTGGCCGG TGGTTAGGGA AGGGCATGGA TGATGGAAGC
    CTGGAGCGGA TCCTAGTTGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCA
    TGCCGGACCC CGCCGCTGCA GCAGTCCCCC AGTGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA AGCCCAAGCT GAACACTGGG CAGATCCAGG AGTCCATCGG GGAGGCAGTC
    AATGGCATTG TGAAGCACTT CCATAAGCCT GAGAAAGAGC GAGGCAGTCT GACGCTGTTG
    CTCTGTGGAG AGTGTGGCCT TGTCTCGGCC TTGGAACAGG CTTTCCAGCA TGGATTTAAA
    TCGCCCCGGC TCTTCAAAAA TGTCTTCATT TGGGATTTCC TGGAAAAAGC ACAAACCTAT
    TATGAGACAT TAGAGAAGAA TGAAGTAGTC CCTGAGGAAA ACTGGCATAC AAGAGCCCGG
    AACTTCTGCC GATTTGTCAC TGCAATCAAC AATACTCCCC GGAACATCGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGATCACC TCCTACACCA CTGGATTGCC
    CTGCTGGCTG ACTGCCCCAT CACTGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CATACACTTG TCAATTCCTT GATTCGTGTG CTGCAGACAT TGCAGGAGTT CAACATCACG
    CTGGAGACGT CCCTTGTCAA GGGCATCGAC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100922
    Clone ID Related Accession (Same CDS sequence) NM_001348750.1 , NM_001348750.2
    Accession Version NM_001348750.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, AP006259.1 and AC079296.5. On May 31, 2019 this sequence version replaced NM_001348750.1. Transcript Variant: This variant (4) has an additional alternate exon in its 5' end which results in the use of a downstream in-frame start codon, compared to variant 1, and encodes a shorter isoform (4), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.267992.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGTA TGCCGAAAGG GCTGGCATTC AAGACCCAGG CTGATCCCAG GGAGCCCCAA 
    TTCCATGCCT TTATTATCAC AAGGGAGGAT GGCTCTCGGA CATTTGGGTT TGCCCTCACA
    TTTTATGAAG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CACAATGCTG AGTATGATGT CCTACATGCT CCCCCTGCTG ATGACAGAGA CCAGAGCAGC
    ATGGAGGATG GTGAAGACAC TCCTGTGACC AAACTGCAGC GCTTCAACTC CTATGACATT
    AGCCGGGACA CTCTCTACGT CTCTAAGTGC ATCTGCCTCA TCACACCCAT GTCTTTCATG
    AAGGCATGTC GGAGCGTGCT GGAGCAACTC CACCAGGCAG TCACTTCACC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAC GTACTCTACG AGGTGCCGCT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGGGTCTAT GGGCCAATAA TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTATTTGA CTTTCCTGTC AAAGAGGTTT TTGAACTGCT CGGGGTGGAG
    AATGTGTTTC AGCTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGA GACTGATGAC TGTGGCGGAG ACGATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTCCC TATTCTCCCA GCTTCTCTCC TGCATTTCTT AGATGCTCCT
    GTTCCATACC TGATGGGTTT GCATTCCAAT GGCCTGGATG ACCGGTCAAA GCTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTGCCAGAG
    GACTTGCCAC AGTTCCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGAA TTCCCCCTGA AGGGAATCTT CATTGCAGTG AGAGTGCCTC CAAGCTGAAG
    AGGCTGCGGG CCTCTGAGCT TGTCTCGGAC AAGAGGAATG GGAACATTGC TGGCTCCCCT
    TTGCATTCCT ACGAGCTTCT TAAGGAGAAT GAAACTATTG CCCGGCTGCA AGCCTTGGTC
    AAGAGAACTG GGGTGAGCCT GGAAAAGTTG GAAGTGCGTG AAGACCCCAG CAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATC
    CAACCCAGCC AGGATAAGGA ATCCTGGTTT ACCAACAGGG AGCAAATGCA AAACTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAG CCCTACCTGC CCTTCCTCTC AAGATTCCTG
    GAGACCCAGA TGTTTGCATC TTTCATTGAC AACAAAATAA TGTGTCATGA TGATGATGAT
    AAAGACCCTG TACTCCGGGT ATTTGATTCC CGAGTTGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTCCGTAC ATCCATGTAC CAGAAGTGTA CCACTGTGGA TGAAGCAGAG
    AAAGCAATTG AGCTGCGTCT GGCAAAAATT GACCATACTG CAATTCACCC ACATTTACTT
    GACATGAAGA TTGGACAAGG GAAATATGAG CCGGGCTTCT TCCCTAAGCT GCAGTCTGAT
    GTACTTTCCA CTGGGCCAGC CAGCAACAAG TGGACGAAAA GGAATGCCCC TGCCCAGTGG
    AGGCGGAAAG ATCGGCAGAA GCAGCACACA GAACACCTGC GTTTAGATAA TGACCAGAGG
    GAGAAGTACA TCCAGGAAGC CAGGACTATG GGCAGCACTA TCCGCCAGCC CAAACTGTCC
    AACCTCTCTC CATCAGTGAT TGCCCAGACC AATTGGAAGT TTGTAGAGGG CCTGCTGAAG
    GAATGCCGCA ATAAGACCAA GAGGATGCTG GTGGAAAAGA TGGGCCGAGA AGCTGTGGAG
    CTAGGGCATG GGGAGGTGAA CATCACAGGG GTGGAAGAGA ACACCCTGAT TGCCAGCCTT
    TGTGATCTCC TGGAAAGGAT CTGGAGTCAT GGACTACAAG TGAAACAGGG GAAATCAGCC
    TTATGGTCCC ACCTGTTACA TTATCAGGAC AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATACT TCTTGATTCA GAACGTAGGA AGTCTGATGC CAGCTCACTC
    ATGCCTCCCC TGAGGATCTC CCTGATTCAG GATATGAGGC ACATCCAGAA CATCGGGGAA
    ATCAAGACTG ATGTGGGAAA GGCCAGAGCA TGGGTGCGAC TGTCCATGGA AAAAAAGTTA
    CTTTCCAGAC ACCTGAAGCA GCTCCTCTCA GACCATGAGC TCACCAAAAA GTTATATAAG
    CGCTATGCCT TCCTGCGCTG TGATGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTGTCT
    TTCAATGCCG TCGATTACTT TTGCTTCACC AATGTCTTCA CAACTATCCT GATCCCGTAC
    CACATTCTGA TCGTACCAAG CAAGAAGCTG GGGGGCTCCA TGTTCACTGC CAACCCATGG
    ATCTGTATAT CAGGAGAATT GGGTGAGACA CAGATCATGC AGATTCCCAG GAATGTGCTA
    GAGATGACCT TCGAGTGCCA GAACTTGGGG AAGCTTACTA CTGTCCAGAT TGGCCATGAT
    AACTCTGGGC TGTATGCCAA ATGGCTGGTG GAGTATGTGA TGGTCAGGAA TGAGATCACA
    GGACATACCT ACAAGTTCCC GTGTGGCCGG TGGTTAGGGA AGGGCATGGA TGATGGAAGC
    CTGGAGCGGA TCCTAGTTGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCA
    TGCCGGACCC CGCCGCTGCA GCAGTCCCCC AGTGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA AGCCCAAGCT GAACACTGGG CAGATCCAGG AGTCCATCGG GGAGGCAGTC
    AATGGCATTG TGAAGCACTT CCATAAGCCT GAGAAAGAGC GAGGCAGTCT GACGCTGTTG
    CTCTGTGGAG AGTGTGGCCT TGTCTCGGCC TTGGAACAGG CTTTCCAGCA TGGATTTAAA
    TCGCCCCGGC TCTTCAAAAA TGTCTTCATT TGGGATTTCC TGGAAAAAGC ACAAACCTAT
    TATGAGACAT TAGAGAAGAA TGAAGTAGTC CCTGAGGAAA ACTGGCATAC AAGAGCCCGG
    AACTTCTGCC GATTTGTCAC TGCAATCAAC AATACTCCCC GGAACATCGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGATCACC TCCTACACCA CTGGATTGCC
    CTGCTGGCTG ACTGCCCCAT CACTGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CATACACTTG TCAATTCCTT GATTCGTGTG CTGCAGACAT TGCAGGAGTT CAACATCACG
    CTGGAGACGT CCCTTGTCAA GGGCATCGAC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335679.1
    CDS606..4181
    Translation

    Target ORF information:

    RefSeq Version NM_001348750.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGTA TGCCGAAAGG GCTGGCATTC AAGACCCAGG CTGATCCCAG GGAGCCCCAA 
    TTCCATGCCT TTATTATCAC AAGGGAGGAT GGCTCTCGGA CATTTGGGTT TGCCCTCACA
    TTTTATGAAG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CACAATGCTG AGTATGATGT CCTACATGCT CCCCCTGCTG ATGACAGAGA CCAGAGCAGC
    ATGGAGGATG GTGAAGACAC TCCTGTGACC AAACTGCAGC GCTTCAACTC CTATGACATT
    AGCCGGGACA CTCTCTACGT CTCTAAGTGC ATCTGCCTCA TCACACCCAT GTCTTTCATG
    AAGGCATGTC GGAGCGTGCT GGAGCAACTC CACCAGGCAG TCACTTCACC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAC GTACTCTACG AGGTGCCGCT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGGGTCTAT GGGCCAATAA TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTATTTGA CTTTCCTGTC AAAGAGGTTT TTGAACTGCT CGGGGTGGAG
    AATGTGTTTC AGCTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGA GACTGATGAC TGTGGCGGAG ACGATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTCCC TATTCTCCCA GCTTCTCTCC TGCATTTCTT AGATGCTCCT
    GTTCCATACC TGATGGGTTT GCATTCCAAT GGCCTGGATG ACCGGTCAAA GCTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTGCCAGAG
    GACTTGCCAC AGTTCCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGAA TTCCCCCTGA AGGGAATCTT CATTGCAGTG AGAGTGCCTC CAAGCTGAAG
    AGGCTGCGGG CCTCTGAGCT TGTCTCGGAC AAGAGGAATG GGAACATTGC TGGCTCCCCT
    TTGCATTCCT ACGAGCTTCT TAAGGAGAAT GAAACTATTG CCCGGCTGCA AGCCTTGGTC
    AAGAGAACTG GGGTGAGCCT GGAAAAGTTG GAAGTGCGTG AAGACCCCAG CAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATC
    CAACCCAGCC AGGATAAGGA ATCCTGGTTT ACCAACAGGG AGCAAATGCA AAACTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAG CCCTACCTGC CCTTCCTCTC AAGATTCCTG
    GAGACCCAGA TGTTTGCATC TTTCATTGAC AACAAAATAA TGTGTCATGA TGATGATGAT
    AAAGACCCTG TACTCCGGGT ATTTGATTCC CGAGTTGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTCCGTAC ATCCATGTAC CAGAAGTGTA CCACTGTGGA TGAAGCAGAG
    AAAGCAATTG AGCTGCGTCT GGCAAAAATT GACCATACTG CAATTCACCC ACATTTACTT
    GACATGAAGA TTGGACAAGG GAAATATGAG CCGGGCTTCT TCCCTAAGCT GCAGTCTGAT
    GTACTTTCCA CTGGGCCAGC CAGCAACAAG TGGACGAAAA GGAATGCCCC TGCCCAGTGG
    AGGCGGAAAG ATCGGCAGAA GCAGCACACA GAACACCTGC GTTTAGATAA TGACCAGAGG
    GAGAAGTACA TCCAGGAAGC CAGGACTATG GGCAGCACTA TCCGCCAGCC CAAACTGTCC
    AACCTCTCTC CATCAGTGAT TGCCCAGACC AATTGGAAGT TTGTAGAGGG CCTGCTGAAG
    GAATGCCGCA ATAAGACCAA GAGGATGCTG GTGGAAAAGA TGGGCCGAGA AGCTGTGGAG
    CTAGGGCATG GGGAGGTGAA CATCACAGGG GTGGAAGAGA ACACCCTGAT TGCCAGCCTT
    TGTGATCTCC TGGAAAGGAT CTGGAGTCAT GGACTACAAG TGAAACAGGG GAAATCAGCC
    TTATGGTCCC ACCTGTTACA TTATCAGGAC AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATACT TCTTGATTCA GAACGTAGGA AGTCTGATGC CAGCTCACTC
    ATGCCTCCCC TGAGGATCTC CCTGATTCAG GATATGAGGC ACATCCAGAA CATCGGGGAA
    ATCAAGACTG ATGTGGGAAA GGCCAGAGCA TGGGTGCGAC TGTCCATGGA AAAAAAGTTA
    CTTTCCAGAC ACCTGAAGCA GCTCCTCTCA GACCATGAGC TCACCAAAAA GTTATATAAG
    CGCTATGCCT TCCTGCGCTG TGATGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTGTCT
    TTCAATGCCG TCGATTACTT TTGCTTCACC AATGTCTTCA CAACTATCCT GATCCCGTAC
    CACATTCTGA TCGTACCAAG CAAGAAGCTG GGGGGCTCCA TGTTCACTGC CAACCCATGG
    ATCTGTATAT CAGGAGAATT GGGTGAGACA CAGATCATGC AGATTCCCAG GAATGTGCTA
    GAGATGACCT TCGAGTGCCA GAACTTGGGG AAGCTTACTA CTGTCCAGAT TGGCCATGAT
    AACTCTGGGC TGTATGCCAA ATGGCTGGTG GAGTATGTGA TGGTCAGGAA TGAGATCACA
    GGACATACCT ACAAGTTCCC GTGTGGCCGG TGGTTAGGGA AGGGCATGGA TGATGGAAGC
    CTGGAGCGGA TCCTAGTTGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCA
    TGCCGGACCC CGCCGCTGCA GCAGTCCCCC AGTGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA AGCCCAAGCT GAACACTGGG CAGATCCAGG AGTCCATCGG GGAGGCAGTC
    AATGGCATTG TGAAGCACTT CCATAAGCCT GAGAAAGAGC GAGGCAGTCT GACGCTGTTG
    CTCTGTGGAG AGTGTGGCCT TGTCTCGGCC TTGGAACAGG CTTTCCAGCA TGGATTTAAA
    TCGCCCCGGC TCTTCAAAAA TGTCTTCATT TGGGATTTCC TGGAAAAAGC ACAAACCTAT
    TATGAGACAT TAGAGAAGAA TGAAGTAGTC CCTGAGGAAA ACTGGCATAC AAGAGCCCGG
    AACTTCTGCC GATTTGTCAC TGCAATCAAC AATACTCCCC GGAACATCGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGATCACC TCCTACACCA CTGGATTGCC
    CTGCTGGCTG ACTGCCCCAT CACTGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CATACACTTG TCAATTCCTT GATTCGTGTG CTGCAGACAT TGCAGGAGTT CAACATCACG
    CTGGAGACGT CCCTTGTCAA GGGCATCGAC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100924
    Clone ID Related Accession (Same CDS sequence) NM_001348749.1 , NM_001348749.2
    Accession Version NM_001348749.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, AP006259.1 and AC079296.5. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, compared to variant 1, and encodes a shorter isoform (3), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.203217.1, SRR1660803.12577.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGG AGAAAATTTT
    GAGCAGACAC CATTGAGAAG AACATTCAAA TCTAAGGTCC TTGCACGATA TCCTGAGAAC
    GTAGAATGGA ATCCCTTTGA CCAAGATGCA GTAGGAATGC TATGTATGCC GAAAGGGCTG
    GCATTCAAGA CCCAGGCTGA TCCCAGGGAG CCCCAATTCC ATGCCTTTAT TATCACAAGG
    GAGGATGGCT CTCGGACATT TGGGTTTGCC CTCACATTTT ATGAAGAGGT GACTAGCAAG
    CAGATCTGCA GTGCAATGCA GACCCTCTAC CACATGCACA ATGCTGAGTA TGATGTCCTA
    CATGCTCCCC CTGCTGATGA CAGAGACCAG AGCAGCATGG AGGATGGTGA AGACACTCCT
    GTGACCAAAC TGCAGCGCTT CAACTCCTAT GACATTAGCC GGGACACTCT CTACGTCTCT
    AAGTGCATCT GCCTCATCAC ACCCATGTCT TTCATGAAGG CATGTCGGAG CGTGCTGGAG
    CAACTCCACC AGGCAGTCAC TTCACCTCAG CCCCCTCCAC TGCCCCTTGA GAGCTACATA
    TACAACGTAC TCTACGAGGT GCCGCTCCCA CCTCCTGGCC GGTCCTTGAA GTTTTCTGGG
    GTCTATGGGC CAATAATCTG CCAGAGACCA AGTACCAATG AGCTTCCCCT ATTTGACTTT
    CCTGTCAAAG AGGTTTTTGA ACTGCTCGGG GTGGAGAATG TGTTTCAGCT TTTTACTTGT
    GCCCTTCTGG AGTTTCAAAT CCTGCTCTAC TCACAGCATT ACCAGAGACT GATGACTGTG
    GCGGAGACGA TTACAGCTCT CATGTTTCCT TTCCAGTGGC AGCATGTCTA TGTCCCTATT
    CTCCCAGCTT CTCTCCTGCA TTTCTTAGAT GCTCCTGTTC CATACCTGAT GGGTTTGCAT
    TCCAATGGCC TGGATGACCG GTCAAAGCTG GAGCTGCCTC AAGAGGCTAA CCTCTGCTTT
    GTGGACATTG ACAACCACTT CATTGAGTTG CCAGAGGACT TGCCACAGTT CCCCAACAAA
    TTGGAGTTTG TCCAGGAAGT CTCTGAGATT CTCATGGCAT TTGGAATTCC CCCTGAAGGG
    AATCTTCATT GCAGTGAGAG TGCCTCCAAG CTGAAGAGGC TGCGGGCCTC TGAGCTTGTC
    TCGGACAAGA GGAATGGGAA CATTGCTGGC TCCCCTTTGC ATTCCTACGA GCTTCTTAAG
    GAGAATGAAA CTATTGCCCG GCTGCAAGCC TTGGTCAAGA GAACTGGGGT GAGCCTGGAA
    AAGTTGGAAG TGCGTGAAGA CCCCAGCAGC AATAAGGATC TCAAAGTTCA GTGTGATGAA
    GAAGAACTCA GGATTTACCA GCTAAACATT CAGATCCGGG AAGTTTTTGC AAATCGTTTC
    ACTCAGATGT TTGCAGATTA TGAGGTGTTT GTCATCCAAC CCAGCCAGGA TAAGGAATCC
    TGGTTTACCA ACAGGGAGCA AATGCAAAAC TTTGATAAAG CATCTTTTCT GTCAGATCAG
    CCTGAGCCCT ACCTGCCCTT CCTCTCAAGA TTCCTGGAGA CCCAGATGTT TGCATCTTTC
    ATTGACAACA AAATAATGTG TCATGATGAT GATGATAAAG ACCCTGTACT CCGGGTATTT
    GATTCCCGAG TTGACAAGAT CAGGCTGTTG AATGTTCGGA CACCTACTCT CCGTACATCC
    ATGTACCAGA AGTGTACCAC TGTGGATGAA GCAGAGAAAG CAATTGAGCT GCGTCTGGCA
    AAAATTGACC ATACTGCAAT TCACCCACAT TTACTTGACA TGAAGATTGG ACAAGGGAAA
    TATGAGCCGG GCTTCTTCCC TAAGCTGCAG TCTGATGTAC TTTCCACTGG GCCAGCCAGC
    AACAAGTGGA CGAAAAGGAA TGCCCCTGCC CAGTGGAGGC GGAAAGATCG GCAGAAGCAG
    CACACAGAAC ACCTGCGTTT AGATAATGAC CAGAGGGAGA AGTACATCCA GGAAGCCAGG
    ACTATGGGCA GCACTATCCG CCAGCCCAAA CTGTCCAACC TCTCTCCATC AGTGATTGCC
    CAGACCAATT GGAAGTTTGT AGAGGGCCTG CTGAAGGAAT GCCGCAATAA GACCAAGAGG
    ATGCTGGTGG AAAAGATGGG CCGAGAAGCT GTGGAGCTAG GGCATGGGGA GGTGAACATC
    ACAGGGGTGG AAGAGAACAC CCTGATTGCC AGCCTTTGTG ATCTCCTGGA AAGGATCTGG
    AGTCATGGAC TACAAGTGAA ACAGGGGAAA TCAGCCTTAT GGTCCCACCT GTTACATTAT
    CAGGACAACC GGCAGAGAAA ACTCACATCA GGAAGCCTCA GTACCTCAGG AATACTTCTT
    GATTCAGAAC GTAGGAAGTC TGATGCCAGC TCACTCATGC CTCCCCTGAG GATCTCCCTG
    ATTCAGGATA TGAGGCACAT CCAGAACATC GGGGAAATCA AGACTGATGT GGGAAAGGCC
    AGAGCATGGG TGCGACTGTC CATGGAAAAA AAGTTACTTT CCAGACACCT GAAGCAGCTC
    CTCTCAGACC ATGAGCTCAC CAAAAAGTTA TATAAGCGCT ATGCCTTCCT GCGCTGTGAT
    GACGAGAAGG AGCAGTTCCT CTATCACCTC CTGTCTTTCA ATGCCGTCGA TTACTTTTGC
    TTCACCAATG TCTTCACAAC TATCCTGATC CCGTACCACA TTCTGATCGT ACCAAGCAAG
    AAGCTGGGGG GCTCCATGTT CACTGCCAAC CCATGGATCT GTATATCAGG AGAATTGGGT
    GAGACACAGA TCATGCAGAT TCCCAGGAAT GTGCTAGAGA TGACCTTCGA GTGCCAGAAC
    TTGGGGAAGC TTACTACTGT CCAGATTGGC CATGATAACT CTGGGCTGTA TGCCAAATGG
    CTGGTGGAGT ATGTGATGGT CAGGAATGAG ATCACAGGAC ATACCTACAA GTTCCCGTGT
    GGCCGGTGGT TAGGGAAGGG CATGGATGAT GGAAGCCTGG AGCGGATCCT AGTTGGGGAG
    CTGCTCACAT CCCAGCCTGA GGTGGATGAG AGGCCATGCC GGACCCCGCC GCTGCAGCAG
    TCCCCCAGTG TCATCCGGAG GCTTGTTACC ATCTCACCCA ACAACAAGCC CAAGCTGAAC
    ACTGGGCAGA TCCAGGAGTC CATCGGGGAG GCAGTCAATG GCATTGTGAA GCACTTCCAT
    AAGCCTGAGA AAGAGCGAGG CAGTCTGACG CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC
    TCGGCCTTGG AACAGGCTTT CCAGCATGGA TTTAAATCGC CCCGGCTCTT CAAAAATGTC
    TTCATTTGGG ATTTCCTGGA AAAAGCACAA ACCTATTATG AGACATTAGA GAAGAATGAA
    GTAGTCCCTG AGGAAAACTG GCATACAAGA GCCCGGAACT TCTGCCGATT TGTCACTGCA
    ATCAACAATA CTCCCCGGAA CATCGGCAAG GATGGCAAGT TTCAGATGCT GGTGTGCTTG
    GGAGCCAGAG ATCACCTCCT ACACCACTGG ATTGCCCTGC TGGCTGACTG CCCCATCACT
    GCACACATGT ATGAGGATGT GGCACTGATC AAAGACCATA CACTTGTCAA TTCCTTGATT
    CGTGTGCTGC AGACATTGCA GGAGTTCAAC ATCACGCTGG AGACGTCCCT TGTCAAGGGC
    ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335678.1
    CDS258..4049
    Translation

    Target ORF information:

    RefSeq Version NM_001348749.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGG AGAAAATTTT
    GAGCAGACAC CATTGAGAAG AACATTCAAA TCTAAGGTCC TTGCACGATA TCCTGAGAAC
    GTAGAATGGA ATCCCTTTGA CCAAGATGCA GTAGGAATGC TATGTATGCC GAAAGGGCTG
    GCATTCAAGA CCCAGGCTGA TCCCAGGGAG CCCCAATTCC ATGCCTTTAT TATCACAAGG
    GAGGATGGCT CTCGGACATT TGGGTTTGCC CTCACATTTT ATGAAGAGGT GACTAGCAAG
    CAGATCTGCA GTGCAATGCA GACCCTCTAC CACATGCACA ATGCTGAGTA TGATGTCCTA
    CATGCTCCCC CTGCTGATGA CAGAGACCAG AGCAGCATGG AGGATGGTGA AGACACTCCT
    GTGACCAAAC TGCAGCGCTT CAACTCCTAT GACATTAGCC GGGACACTCT CTACGTCTCT
    AAGTGCATCT GCCTCATCAC ACCCATGTCT TTCATGAAGG CATGTCGGAG CGTGCTGGAG
    CAACTCCACC AGGCAGTCAC TTCACCTCAG CCCCCTCCAC TGCCCCTTGA GAGCTACATA
    TACAACGTAC TCTACGAGGT GCCGCTCCCA CCTCCTGGCC GGTCCTTGAA GTTTTCTGGG
    GTCTATGGGC CAATAATCTG CCAGAGACCA AGTACCAATG AGCTTCCCCT ATTTGACTTT
    CCTGTCAAAG AGGTTTTTGA ACTGCTCGGG GTGGAGAATG TGTTTCAGCT TTTTACTTGT
    GCCCTTCTGG AGTTTCAAAT CCTGCTCTAC TCACAGCATT ACCAGAGACT GATGACTGTG
    GCGGAGACGA TTACAGCTCT CATGTTTCCT TTCCAGTGGC AGCATGTCTA TGTCCCTATT
    CTCCCAGCTT CTCTCCTGCA TTTCTTAGAT GCTCCTGTTC CATACCTGAT GGGTTTGCAT
    TCCAATGGCC TGGATGACCG GTCAAAGCTG GAGCTGCCTC AAGAGGCTAA CCTCTGCTTT
    GTGGACATTG ACAACCACTT CATTGAGTTG CCAGAGGACT TGCCACAGTT CCCCAACAAA
    TTGGAGTTTG TCCAGGAAGT CTCTGAGATT CTCATGGCAT TTGGAATTCC CCCTGAAGGG
    AATCTTCATT GCAGTGAGAG TGCCTCCAAG CTGAAGAGGC TGCGGGCCTC TGAGCTTGTC
    TCGGACAAGA GGAATGGGAA CATTGCTGGC TCCCCTTTGC ATTCCTACGA GCTTCTTAAG
    GAGAATGAAA CTATTGCCCG GCTGCAAGCC TTGGTCAAGA GAACTGGGGT GAGCCTGGAA
    AAGTTGGAAG TGCGTGAAGA CCCCAGCAGC AATAAGGATC TCAAAGTTCA GTGTGATGAA
    GAAGAACTCA GGATTTACCA GCTAAACATT CAGATCCGGG AAGTTTTTGC AAATCGTTTC
    ACTCAGATGT TTGCAGATTA TGAGGTGTTT GTCATCCAAC CCAGCCAGGA TAAGGAATCC
    TGGTTTACCA ACAGGGAGCA AATGCAAAAC TTTGATAAAG CATCTTTTCT GTCAGATCAG
    CCTGAGCCCT ACCTGCCCTT CCTCTCAAGA TTCCTGGAGA CCCAGATGTT TGCATCTTTC
    ATTGACAACA AAATAATGTG TCATGATGAT GATGATAAAG ACCCTGTACT CCGGGTATTT
    GATTCCCGAG TTGACAAGAT CAGGCTGTTG AATGTTCGGA CACCTACTCT CCGTACATCC
    ATGTACCAGA AGTGTACCAC TGTGGATGAA GCAGAGAAAG CAATTGAGCT GCGTCTGGCA
    AAAATTGACC ATACTGCAAT TCACCCACAT TTACTTGACA TGAAGATTGG ACAAGGGAAA
    TATGAGCCGG GCTTCTTCCC TAAGCTGCAG TCTGATGTAC TTTCCACTGG GCCAGCCAGC
    AACAAGTGGA CGAAAAGGAA TGCCCCTGCC CAGTGGAGGC GGAAAGATCG GCAGAAGCAG
    CACACAGAAC ACCTGCGTTT AGATAATGAC CAGAGGGAGA AGTACATCCA GGAAGCCAGG
    ACTATGGGCA GCACTATCCG CCAGCCCAAA CTGTCCAACC TCTCTCCATC AGTGATTGCC
    CAGACCAATT GGAAGTTTGT AGAGGGCCTG CTGAAGGAAT GCCGCAATAA GACCAAGAGG
    ATGCTGGTGG AAAAGATGGG CCGAGAAGCT GTGGAGCTAG GGCATGGGGA GGTGAACATC
    ACAGGGGTGG AAGAGAACAC CCTGATTGCC AGCCTTTGTG ATCTCCTGGA AAGGATCTGG
    AGTCATGGAC TACAAGTGAA ACAGGGGAAA TCAGCCTTAT GGTCCCACCT GTTACATTAT
    CAGGACAACC GGCAGAGAAA ACTCACATCA GGAAGCCTCA GTACCTCAGG AATACTTCTT
    GATTCAGAAC GTAGGAAGTC TGATGCCAGC TCACTCATGC CTCCCCTGAG GATCTCCCTG
    ATTCAGGATA TGAGGCACAT CCAGAACATC GGGGAAATCA AGACTGATGT GGGAAAGGCC
    AGAGCATGGG TGCGACTGTC CATGGAAAAA AAGTTACTTT CCAGACACCT GAAGCAGCTC
    CTCTCAGACC ATGAGCTCAC CAAAAAGTTA TATAAGCGCT ATGCCTTCCT GCGCTGTGAT
    GACGAGAAGG AGCAGTTCCT CTATCACCTC CTGTCTTTCA ATGCCGTCGA TTACTTTTGC
    TTCACCAATG TCTTCACAAC TATCCTGATC CCGTACCACA TTCTGATCGT ACCAAGCAAG
    AAGCTGGGGG GCTCCATGTT CACTGCCAAC CCATGGATCT GTATATCAGG AGAATTGGGT
    GAGACACAGA TCATGCAGAT TCCCAGGAAT GTGCTAGAGA TGACCTTCGA GTGCCAGAAC
    TTGGGGAAGC TTACTACTGT CCAGATTGGC CATGATAACT CTGGGCTGTA TGCCAAATGG
    CTGGTGGAGT ATGTGATGGT CAGGAATGAG ATCACAGGAC ATACCTACAA GTTCCCGTGT
    GGCCGGTGGT TAGGGAAGGG CATGGATGAT GGAAGCCTGG AGCGGATCCT AGTTGGGGAG
    CTGCTCACAT CCCAGCCTGA GGTGGATGAG AGGCCATGCC GGACCCCGCC GCTGCAGCAG
    TCCCCCAGTG TCATCCGGAG GCTTGTTACC ATCTCACCCA ACAACAAGCC CAAGCTGAAC
    ACTGGGCAGA TCCAGGAGTC CATCGGGGAG GCAGTCAATG GCATTGTGAA GCACTTCCAT
    AAGCCTGAGA AAGAGCGAGG CAGTCTGACG CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC
    TCGGCCTTGG AACAGGCTTT CCAGCATGGA TTTAAATCGC CCCGGCTCTT CAAAAATGTC
    TTCATTTGGG ATTTCCTGGA AAAAGCACAA ACCTATTATG AGACATTAGA GAAGAATGAA
    GTAGTCCCTG AGGAAAACTG GCATACAAGA GCCCGGAACT TCTGCCGATT TGTCACTGCA
    ATCAACAATA CTCCCCGGAA CATCGGCAAG GATGGCAAGT TTCAGATGCT GGTGTGCTTG
    GGAGCCAGAG ATCACCTCCT ACACCACTGG ATTGCCCTGC TGGCTGACTG CCCCATCACT
    GCACACATGT ATGAGGATGT GGCACTGATC AAAGACCATA CACTTGTCAA TTCCTTGATT
    CGTGTGCTGC AGACATTGCA GGAGTTCAAC ATCACGCTGG AGACGTCCCT TGTCAAGGGC
    ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100924
    Clone ID Related Accession (Same CDS sequence) NM_001348749.1 , NM_001348749.2
    Accession Version NM_001348749.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, AP006259.1 and AC079296.5. On May 31, 2019 this sequence version replaced NM_001348749.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, compared to variant 1, and encodes a shorter isoform (3), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.203217.1, SRR1660803.12577.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGG AGAAAATTTT
    GAGCAGACAC CATTGAGAAG AACATTCAAA TCTAAGGTCC TTGCACGATA TCCTGAGAAC
    GTAGAATGGA ATCCCTTTGA CCAAGATGCA GTAGGAATGC TATGTATGCC GAAAGGGCTG
    GCATTCAAGA CCCAGGCTGA TCCCAGGGAG CCCCAATTCC ATGCCTTTAT TATCACAAGG
    GAGGATGGCT CTCGGACATT TGGGTTTGCC CTCACATTTT ATGAAGAGGT GACTAGCAAG
    CAGATCTGCA GTGCAATGCA GACCCTCTAC CACATGCACA ATGCTGAGTA TGATGTCCTA
    CATGCTCCCC CTGCTGATGA CAGAGACCAG AGCAGCATGG AGGATGGTGA AGACACTCCT
    GTGACCAAAC TGCAGCGCTT CAACTCCTAT GACATTAGCC GGGACACTCT CTACGTCTCT
    AAGTGCATCT GCCTCATCAC ACCCATGTCT TTCATGAAGG CATGTCGGAG CGTGCTGGAG
    CAACTCCACC AGGCAGTCAC TTCACCTCAG CCCCCTCCAC TGCCCCTTGA GAGCTACATA
    TACAACGTAC TCTACGAGGT GCCGCTCCCA CCTCCTGGCC GGTCCTTGAA GTTTTCTGGG
    GTCTATGGGC CAATAATCTG CCAGAGACCA AGTACCAATG AGCTTCCCCT ATTTGACTTT
    CCTGTCAAAG AGGTTTTTGA ACTGCTCGGG GTGGAGAATG TGTTTCAGCT TTTTACTTGT
    GCCCTTCTGG AGTTTCAAAT CCTGCTCTAC TCACAGCATT ACCAGAGACT GATGACTGTG
    GCGGAGACGA TTACAGCTCT CATGTTTCCT TTCCAGTGGC AGCATGTCTA TGTCCCTATT
    CTCCCAGCTT CTCTCCTGCA TTTCTTAGAT GCTCCTGTTC CATACCTGAT GGGTTTGCAT
    TCCAATGGCC TGGATGACCG GTCAAAGCTG GAGCTGCCTC AAGAGGCTAA CCTCTGCTTT
    GTGGACATTG ACAACCACTT CATTGAGTTG CCAGAGGACT TGCCACAGTT CCCCAACAAA
    TTGGAGTTTG TCCAGGAAGT CTCTGAGATT CTCATGGCAT TTGGAATTCC CCCTGAAGGG
    AATCTTCATT GCAGTGAGAG TGCCTCCAAG CTGAAGAGGC TGCGGGCCTC TGAGCTTGTC
    TCGGACAAGA GGAATGGGAA CATTGCTGGC TCCCCTTTGC ATTCCTACGA GCTTCTTAAG
    GAGAATGAAA CTATTGCCCG GCTGCAAGCC TTGGTCAAGA GAACTGGGGT GAGCCTGGAA
    AAGTTGGAAG TGCGTGAAGA CCCCAGCAGC AATAAGGATC TCAAAGTTCA GTGTGATGAA
    GAAGAACTCA GGATTTACCA GCTAAACATT CAGATCCGGG AAGTTTTTGC AAATCGTTTC
    ACTCAGATGT TTGCAGATTA TGAGGTGTTT GTCATCCAAC CCAGCCAGGA TAAGGAATCC
    TGGTTTACCA ACAGGGAGCA AATGCAAAAC TTTGATAAAG CATCTTTTCT GTCAGATCAG
    CCTGAGCCCT ACCTGCCCTT CCTCTCAAGA TTCCTGGAGA CCCAGATGTT TGCATCTTTC
    ATTGACAACA AAATAATGTG TCATGATGAT GATGATAAAG ACCCTGTACT CCGGGTATTT
    GATTCCCGAG TTGACAAGAT CAGGCTGTTG AATGTTCGGA CACCTACTCT CCGTACATCC
    ATGTACCAGA AGTGTACCAC TGTGGATGAA GCAGAGAAAG CAATTGAGCT GCGTCTGGCA
    AAAATTGACC ATACTGCAAT TCACCCACAT TTACTTGACA TGAAGATTGG ACAAGGGAAA
    TATGAGCCGG GCTTCTTCCC TAAGCTGCAG TCTGATGTAC TTTCCACTGG GCCAGCCAGC
    AACAAGTGGA CGAAAAGGAA TGCCCCTGCC CAGTGGAGGC GGAAAGATCG GCAGAAGCAG
    CACACAGAAC ACCTGCGTTT AGATAATGAC CAGAGGGAGA AGTACATCCA GGAAGCCAGG
    ACTATGGGCA GCACTATCCG CCAGCCCAAA CTGTCCAACC TCTCTCCATC AGTGATTGCC
    CAGACCAATT GGAAGTTTGT AGAGGGCCTG CTGAAGGAAT GCCGCAATAA GACCAAGAGG
    ATGCTGGTGG AAAAGATGGG CCGAGAAGCT GTGGAGCTAG GGCATGGGGA GGTGAACATC
    ACAGGGGTGG AAGAGAACAC CCTGATTGCC AGCCTTTGTG ATCTCCTGGA AAGGATCTGG
    AGTCATGGAC TACAAGTGAA ACAGGGGAAA TCAGCCTTAT GGTCCCACCT GTTACATTAT
    CAGGACAACC GGCAGAGAAA ACTCACATCA GGAAGCCTCA GTACCTCAGG AATACTTCTT
    GATTCAGAAC GTAGGAAGTC TGATGCCAGC TCACTCATGC CTCCCCTGAG GATCTCCCTG
    ATTCAGGATA TGAGGCACAT CCAGAACATC GGGGAAATCA AGACTGATGT GGGAAAGGCC
    AGAGCATGGG TGCGACTGTC CATGGAAAAA AAGTTACTTT CCAGACACCT GAAGCAGCTC
    CTCTCAGACC ATGAGCTCAC CAAAAAGTTA TATAAGCGCT ATGCCTTCCT GCGCTGTGAT
    GACGAGAAGG AGCAGTTCCT CTATCACCTC CTGTCTTTCA ATGCCGTCGA TTACTTTTGC
    TTCACCAATG TCTTCACAAC TATCCTGATC CCGTACCACA TTCTGATCGT ACCAAGCAAG
    AAGCTGGGGG GCTCCATGTT CACTGCCAAC CCATGGATCT GTATATCAGG AGAATTGGGT
    GAGACACAGA TCATGCAGAT TCCCAGGAAT GTGCTAGAGA TGACCTTCGA GTGCCAGAAC
    TTGGGGAAGC TTACTACTGT CCAGATTGGC CATGATAACT CTGGGCTGTA TGCCAAATGG
    CTGGTGGAGT ATGTGATGGT CAGGAATGAG ATCACAGGAC ATACCTACAA GTTCCCGTGT
    GGCCGGTGGT TAGGGAAGGG CATGGATGAT GGAAGCCTGG AGCGGATCCT AGTTGGGGAG
    CTGCTCACAT CCCAGCCTGA GGTGGATGAG AGGCCATGCC GGACCCCGCC GCTGCAGCAG
    TCCCCCAGTG TCATCCGGAG GCTTGTTACC ATCTCACCCA ACAACAAGCC CAAGCTGAAC
    ACTGGGCAGA TCCAGGAGTC CATCGGGGAG GCAGTCAATG GCATTGTGAA GCACTTCCAT
    AAGCCTGAGA AAGAGCGAGG CAGTCTGACG CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC
    TCGGCCTTGG AACAGGCTTT CCAGCATGGA TTTAAATCGC CCCGGCTCTT CAAAAATGTC
    TTCATTTGGG ATTTCCTGGA AAAAGCACAA ACCTATTATG AGACATTAGA GAAGAATGAA
    GTAGTCCCTG AGGAAAACTG GCATACAAGA GCCCGGAACT TCTGCCGATT TGTCACTGCA
    ATCAACAATA CTCCCCGGAA CATCGGCAAG GATGGCAAGT TTCAGATGCT GGTGTGCTTG
    GGAGCCAGAG ATCACCTCCT ACACCACTGG ATTGCCCTGC TGGCTGACTG CCCCATCACT
    GCACACATGT ATGAGGATGT GGCACTGATC AAAGACCATA CACTTGTCAA TTCCTTGATT
    CGTGTGCTGC AGACATTGCA GGAGTTCAAC ATCACGCTGG AGACGTCCCT TGTCAAGGGC
    ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335678.1
    CDS282..4073
    Translation

    Target ORF information:

    RefSeq Version NM_001348749.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348749.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGG AGAAAATTTT
    GAGCAGACAC CATTGAGAAG AACATTCAAA TCTAAGGTCC TTGCACGATA TCCTGAGAAC
    GTAGAATGGA ATCCCTTTGA CCAAGATGCA GTAGGAATGC TATGTATGCC GAAAGGGCTG
    GCATTCAAGA CCCAGGCTGA TCCCAGGGAG CCCCAATTCC ATGCCTTTAT TATCACAAGG
    GAGGATGGCT CTCGGACATT TGGGTTTGCC CTCACATTTT ATGAAGAGGT GACTAGCAAG
    CAGATCTGCA GTGCAATGCA GACCCTCTAC CACATGCACA ATGCTGAGTA TGATGTCCTA
    CATGCTCCCC CTGCTGATGA CAGAGACCAG AGCAGCATGG AGGATGGTGA AGACACTCCT
    GTGACCAAAC TGCAGCGCTT CAACTCCTAT GACATTAGCC GGGACACTCT CTACGTCTCT
    AAGTGCATCT GCCTCATCAC ACCCATGTCT TTCATGAAGG CATGTCGGAG CGTGCTGGAG
    CAACTCCACC AGGCAGTCAC TTCACCTCAG CCCCCTCCAC TGCCCCTTGA GAGCTACATA
    TACAACGTAC TCTACGAGGT GCCGCTCCCA CCTCCTGGCC GGTCCTTGAA GTTTTCTGGG
    GTCTATGGGC CAATAATCTG CCAGAGACCA AGTACCAATG AGCTTCCCCT ATTTGACTTT
    CCTGTCAAAG AGGTTTTTGA ACTGCTCGGG GTGGAGAATG TGTTTCAGCT TTTTACTTGT
    GCCCTTCTGG AGTTTCAAAT CCTGCTCTAC TCACAGCATT ACCAGAGACT GATGACTGTG
    GCGGAGACGA TTACAGCTCT CATGTTTCCT TTCCAGTGGC AGCATGTCTA TGTCCCTATT
    CTCCCAGCTT CTCTCCTGCA TTTCTTAGAT GCTCCTGTTC CATACCTGAT GGGTTTGCAT
    TCCAATGGCC TGGATGACCG GTCAAAGCTG GAGCTGCCTC AAGAGGCTAA CCTCTGCTTT
    GTGGACATTG ACAACCACTT CATTGAGTTG CCAGAGGACT TGCCACAGTT CCCCAACAAA
    TTGGAGTTTG TCCAGGAAGT CTCTGAGATT CTCATGGCAT TTGGAATTCC CCCTGAAGGG
    AATCTTCATT GCAGTGAGAG TGCCTCCAAG CTGAAGAGGC TGCGGGCCTC TGAGCTTGTC
    TCGGACAAGA GGAATGGGAA CATTGCTGGC TCCCCTTTGC ATTCCTACGA GCTTCTTAAG
    GAGAATGAAA CTATTGCCCG GCTGCAAGCC TTGGTCAAGA GAACTGGGGT GAGCCTGGAA
    AAGTTGGAAG TGCGTGAAGA CCCCAGCAGC AATAAGGATC TCAAAGTTCA GTGTGATGAA
    GAAGAACTCA GGATTTACCA GCTAAACATT CAGATCCGGG AAGTTTTTGC AAATCGTTTC
    ACTCAGATGT TTGCAGATTA TGAGGTGTTT GTCATCCAAC CCAGCCAGGA TAAGGAATCC
    TGGTTTACCA ACAGGGAGCA AATGCAAAAC TTTGATAAAG CATCTTTTCT GTCAGATCAG
    CCTGAGCCCT ACCTGCCCTT CCTCTCAAGA TTCCTGGAGA CCCAGATGTT TGCATCTTTC
    ATTGACAACA AAATAATGTG TCATGATGAT GATGATAAAG ACCCTGTACT CCGGGTATTT
    GATTCCCGAG TTGACAAGAT CAGGCTGTTG AATGTTCGGA CACCTACTCT CCGTACATCC
    ATGTACCAGA AGTGTACCAC TGTGGATGAA GCAGAGAAAG CAATTGAGCT GCGTCTGGCA
    AAAATTGACC ATACTGCAAT TCACCCACAT TTACTTGACA TGAAGATTGG ACAAGGGAAA
    TATGAGCCGG GCTTCTTCCC TAAGCTGCAG TCTGATGTAC TTTCCACTGG GCCAGCCAGC
    AACAAGTGGA CGAAAAGGAA TGCCCCTGCC CAGTGGAGGC GGAAAGATCG GCAGAAGCAG
    CACACAGAAC ACCTGCGTTT AGATAATGAC CAGAGGGAGA AGTACATCCA GGAAGCCAGG
    ACTATGGGCA GCACTATCCG CCAGCCCAAA CTGTCCAACC TCTCTCCATC AGTGATTGCC
    CAGACCAATT GGAAGTTTGT AGAGGGCCTG CTGAAGGAAT GCCGCAATAA GACCAAGAGG
    ATGCTGGTGG AAAAGATGGG CCGAGAAGCT GTGGAGCTAG GGCATGGGGA GGTGAACATC
    ACAGGGGTGG AAGAGAACAC CCTGATTGCC AGCCTTTGTG ATCTCCTGGA AAGGATCTGG
    AGTCATGGAC TACAAGTGAA ACAGGGGAAA TCAGCCTTAT GGTCCCACCT GTTACATTAT
    CAGGACAACC GGCAGAGAAA ACTCACATCA GGAAGCCTCA GTACCTCAGG AATACTTCTT
    GATTCAGAAC GTAGGAAGTC TGATGCCAGC TCACTCATGC CTCCCCTGAG GATCTCCCTG
    ATTCAGGATA TGAGGCACAT CCAGAACATC GGGGAAATCA AGACTGATGT GGGAAAGGCC
    AGAGCATGGG TGCGACTGTC CATGGAAAAA AAGTTACTTT CCAGACACCT GAAGCAGCTC
    CTCTCAGACC ATGAGCTCAC CAAAAAGTTA TATAAGCGCT ATGCCTTCCT GCGCTGTGAT
    GACGAGAAGG AGCAGTTCCT CTATCACCTC CTGTCTTTCA ATGCCGTCGA TTACTTTTGC
    TTCACCAATG TCTTCACAAC TATCCTGATC CCGTACCACA TTCTGATCGT ACCAAGCAAG
    AAGCTGGGGG GCTCCATGTT CACTGCCAAC CCATGGATCT GTATATCAGG AGAATTGGGT
    GAGACACAGA TCATGCAGAT TCCCAGGAAT GTGCTAGAGA TGACCTTCGA GTGCCAGAAC
    TTGGGGAAGC TTACTACTGT CCAGATTGGC CATGATAACT CTGGGCTGTA TGCCAAATGG
    CTGGTGGAGT ATGTGATGGT CAGGAATGAG ATCACAGGAC ATACCTACAA GTTCCCGTGT
    GGCCGGTGGT TAGGGAAGGG CATGGATGAT GGAAGCCTGG AGCGGATCCT AGTTGGGGAG
    CTGCTCACAT CCCAGCCTGA GGTGGATGAG AGGCCATGCC GGACCCCGCC GCTGCAGCAG
    TCCCCCAGTG TCATCCGGAG GCTTGTTACC ATCTCACCCA ACAACAAGCC CAAGCTGAAC
    ACTGGGCAGA TCCAGGAGTC CATCGGGGAG GCAGTCAATG GCATTGTGAA GCACTTCCAT
    AAGCCTGAGA AAGAGCGAGG CAGTCTGACG CTGTTGCTCT GTGGAGAGTG TGGCCTTGTC
    TCGGCCTTGG AACAGGCTTT CCAGCATGGA TTTAAATCGC CCCGGCTCTT CAAAAATGTC
    TTCATTTGGG ATTTCCTGGA AAAAGCACAA ACCTATTATG AGACATTAGA GAAGAATGAA
    GTAGTCCCTG AGGAAAACTG GCATACAAGA GCCCGGAACT TCTGCCGATT TGTCACTGCA
    ATCAACAATA CTCCCCGGAA CATCGGCAAG GATGGCAAGT TTCAGATGCT GGTGTGCTTG
    GGAGCCAGAG ATCACCTCCT ACACCACTGG ATTGCCCTGC TGGCTGACTG CCCCATCACT
    GCACACATGT ATGAGGATGT GGCACTGATC AAAGACCATA CACTTGTCAA TTCCTTGATT
    CGTGTGCTGC AGACATTGCA GGAGTTCAAC ATCACGCTGG AGACGTCCCT TGTCAAGGGC
    ATCGACATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02229
    Clone ID Related Accession (Same CDS sequence) NM_001243254.1 , NM_001243254.2
    Accession Version NM_001243254.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN999250.1, AK295881.1 and AL117448.1. Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK295881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGAGAT CACCTCCTAC
    ACCACTGGAT TGCCCTGCTG GCTGACTGCC CCATCACTGC ACACATGTAT GAGGATGTGG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230183.1
    CDS258..3983
    Translation

    Target ORF information:

    RefSeq Version NM_001243254.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGAGAT CACCTCCTAC
    ACCACTGGAT TGCCCTGCTG GCTGACTGCC CCATCACTGC ACACATGTAT GAGGATGTGG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02229
    Clone ID Related Accession (Same CDS sequence) NM_001243254.1 , NM_001243254.2
    Accession Version NM_001243254.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, DN999250.1, AK295881.1 and AL117448.1. On May 31, 2019 this sequence version replaced NM_001243254.1. Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK295881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGAGAT CACCTCCTAC
    ACCACTGGAT TGCCCTGCTG GCTGACTGCC CCATCACTGC ACACATGTAT GAGGATGTGG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230183.1
    CDS282..4007
    Translation

    Target ORF information:

    RefSeq Version NM_001243254.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243254.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGAGAT CACCTCCTAC
    ACCACTGGAT TGCCCTGCTG GCTGACTGCC CCATCACTGC ACACATGTAT GAGGATGTGG
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06879
    Clone ID Related Accession (Same CDS sequence) NM_001348748.1 , NM_015213.4 , NM_015213.3
    Accession Version NM_001348748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, AP006259.1 and AC079296.5. Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1, and encodes the longest isoform (1). Variants 1 and 5 encode the same protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC071596.1, SRR1803614.104931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001335677.1
    CDS80..3943
    Translation

    Target ORF information:

    RefSeq Version NM_001348748.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001348748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06879
    Clone ID Related Accession (Same CDS sequence) NM_001348748.1 , NM_015213.4 , NM_015213.3
    Accession Version NM_015213.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055845.15, DN999250.1, AB029014.1 and AL117448.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015213.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AL117448.1, SRR1660803.41769.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328194.8/ ENSP00000328524.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056028.2
    CDS282..4145
    Translation

    Target ORF information:

    RefSeq Version NM_015213.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015213.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06879
    Clone ID Related Accession (Same CDS sequence) NM_001348748.1 , NM_015213.4 , NM_015213.3
    Accession Version NM_015213.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN999250.1, AB029014.1 and AL117448.1. This sequence is a reference standard in the RefSeqGene project. On Aug 6, 2011 this sequence version replaced NM_015213.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AL117448.1, SRR1660803.41769.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056028.2
    CDS258..4121
    Translation

    Target ORF information:

    RefSeq Version NM_015213.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44799
    Clone ID Related Accession (Same CDS sequence) XM_005252832.1
    Accession Version XM_005252832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DENN domain-containing protein 5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG GGTTATCATG AAGATTAAAT CTCTTATGTC AGTGCCAGCA
    CATGCTGAGT GTTATGTGAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005252889.1
    CDS267..4190
    Translation

    Target ORF information:

    RefSeq Version XM_005252832.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005252832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGTGGCG GCGGCGGCGG AGGGGGCTCG GCGCCCAGTC GCTTCGCCGA CTACTTTGTC 
    ATCTGCGGAC TGGACACGGA GACCGGGCTG GAGCCGGACG AGCTGTCGGC ATTATGCCAG
    TACATACAGG CTTCTAAAGC CAGGGATGGT GCCAGCCCTT TCATTTCAAG TACGACTGAA
    GGAGAAAATT TTGAGCAGAC ACCATTGAGA AGAACATTCA AATCTAAGGT CCTTGCACGA
    TATCCTGAGA ACGTAGAATG GAATCCCTTT GACCAAGATG CAGTAGGAAT GCTATGTATG
    CCGAAAGGGC TGGCATTCAA GACCCAGGCT GATCCCAGGG AGCCCCAATT CCATGCCTTT
    ATTATCACAA GGGAGGATGG CTCTCGGACA TTTGGGTTTG CCCTCACATT TTATGAAGAG
    GTGACTAGCA AGCAGATCTG CAGTGCAATG CAGACCCTCT ACCACATGCA CAATGCTGAG
    TATGATGTCC TACATGCTCC CCCTGCTGAT GACAGAGACC AGAGCAGCAT GGAGGATGGT
    GAAGACACTC CTGTGACCAA ACTGCAGCGC TTCAACTCCT ATGACATTAG CCGGGACACT
    CTCTACGTCT CTAAGTGCAT CTGCCTCATC ACACCCATGT CTTTCATGAA GGCATGTCGG
    AGCGTGCTGG AGCAACTCCA CCAGGCAGTC ACTTCACCTC AGCCCCCTCC ACTGCCCCTT
    GAGAGCTACA TATACAACGT ACTCTACGAG GTGCCGCTCC CACCTCCTGG CCGGTCCTTG
    AAGTTTTCTG GGGTCTATGG GCCAATAATC TGCCAGAGAC CAAGTACCAA TGAGCTTCCC
    CTATTTGACT TTCCTGTCAA AGAGGTTTTT GAACTGCTCG GGGTGGAGAA TGTGTTTCAG
    CTTTTTACTT GTGCCCTTCT GGAGTTTCAA ATCCTGCTCT ACTCACAGCA TTACCAGAGA
    CTGATGACTG TGGCGGAGAC GATTACAGCT CTCATGTTTC CTTTCCAGTG GCAGCATGTC
    TATGTCCCTA TTCTCCCAGC TTCTCTCCTG CATTTCTTAG ATGCTCCTGT TCCATACCTG
    ATGGGTTTGC ATTCCAATGG CCTGGATGAC CGGTCAAAGC TGGAGCTGCC TCAAGAGGCT
    AACCTCTGCT TTGTGGACAT TGACAACCAC TTCATTGAGT TGCCAGAGGA CTTGCCACAG
    TTCCCCAACA AATTGGAGTT TGTCCAGGAA GTCTCTGAGA TTCTCATGGC ATTTGGAATT
    CCCCCTGAAG GGAATCTTCA TTGCAGTGAG AGTGCCTCCA AGCTGAAGAG GCTGCGGGCC
    TCTGAGCTTG TCTCGGACAA GAGGAATGGG AACATTGCTG GCTCCCCTTT GCATTCCTAC
    GAGCTTCTTA AGGAGAATGA AACTATTGCC CGGCTGCAAG CCTTGGTCAA GAGAACTGGG
    GTGAGCCTGG AAAAGTTGGA AGTGCGTGAA GACCCCAGCA GCAATAAGGA TCTCAAAGTT
    CAGTGTGATG AAGAAGAACT CAGGATTTAC CAGCTAAACA TTCAGATCCG GGAAGTTTTT
    GCAAATCGTT TCACTCAGAT GTTTGCAGAT TATGAGGTGT TTGTCATCCA ACCCAGCCAG
    GATAAGGAAT CCTGGTTTAC CAACAGGGAG CAAATGCAAA ACTTTGATAA AGCATCTTTT
    CTGTCAGATC AGCCTGAGCC CTACCTGCCC TTCCTCTCAA GATTCCTGGA GACCCAGATG
    TTTGCATCTT TCATTGACAA CAAAATAATG TGTCATGATG ATGATGATAA AGACCCTGTA
    CTCCGGGTAT TTGATTCCCG AGTTGACAAG ATCAGGCTGT TGAATGTTCG GACACCTACT
    CTCCGTACAT CCATGTACCA GAAGTGTACC ACTGTGGATG AAGCAGAGAA AGCAATTGAG
    CTGCGTCTGG CAAAAATTGA CCATACTGCA ATTCACCCAC ATTTACTTGA CATGAAGATT
    GGACAAGGGA AATATGAGCC GGGCTTCTTC CCTAAGCTGC AGTCTGATGT ACTTTCCACT
    GGGCCAGCCA GCAACAAGTG GACGAAAAGG AATGCCCCTG CCCAGTGGAG GCGGAAAGAT
    CGGCAGAAGC AGCACACAGA ACACCTGCGT TTAGATAATG ACCAGAGGGA GAAGTACATC
    CAGGAAGCCA GGACTATGGG CAGCACTATC CGCCAGCCCA AACTGTCCAA CCTCTCTCCA
    TCAGTGATTG CCCAGACCAA TTGGAAGTTT GTAGAGGGCC TGCTGAAGGA ATGCCGCAAT
    AAGACCAAGA GGATGCTGGT GGAAAAGATG GGCCGAGAAG CTGTGGAGCT AGGGCATGGG
    GAGGTGAACA TCACAGGGGT GGAAGAGAAC ACCCTGATTG CCAGCCTTTG TGATCTCCTG
    GAAAGGATCT GGAGTCATGG ACTACAAGTG AAACAGGGGA AATCAGCCTT ATGGTCCCAC
    CTGTTACATT ATCAGGACAA CCGGCAGAGA AAACTCACAT CAGGAAGCCT CAGTACCTCA
    GGAATACTTC TTGATTCAGA ACGTAGGAAG TCTGATGCCA GCTCACTCAT GCCTCCCCTG
    AGGATCTCCC TGATTCAGGA TATGAGGCAC ATCCAGAACA TCGGGGAAAT CAAGACTGAT
    GTGGGAAAGG CCAGAGCATG GGTGCGACTG TCCATGGAAA AAAAGTTACT TTCCAGACAC
    CTGAAGCAGC TCCTCTCAGA CCATGAGCTC ACCAAAAAGT TATATAAGCG CTATGCCTTC
    CTGCGCTGTG ATGACGAGAA GGAGCAGTTC CTCTATCACC TCCTGTCTTT CAATGCCGTC
    GATTACTTTT GCTTCACCAA TGTCTTCACA ACTATCCTGA TCCCGTACCA CATTCTGATC
    GTACCAAGCA AGAAGCTGGG GGGCTCCATG TTCACTGCCA ACCCATGGAT CTGTATATCA
    GGAGAATTGG GTGAGACACA GATCATGCAG ATTCCCAGGA ATGTGCTAGA GATGACCTTC
    GAGTGCCAGA ACTTGGGGAA GCTTACTACT GTCCAGATTG GCCATGATAA CTCTGGGCTG
    TATGCCAAAT GGCTGGTGGA GTATGTGATG GTCAGGAATG AGATCACAGG ACATACCTAC
    AAGTTCCCGT GTGGCCGGTG GTTAGGGAAG GGCATGGATG ATGGAAGCCT GGAGCGGATC
    CTAGTTGGGG AGCTGCTCAC ATCCCAGCCT GAGGTGGATG AGAGGCCATG CCGGACCCCG
    CCGCTGCAGC AGTCCCCCAG TGTCATCCGG AGGCTTGTTA CCATCTCACC CAACAACAAG
    CCCAAGCTGA ACACTGGGCA GATCCAGGAG TCCATCGGGG AGGCAGTCAA TGGCATTGTG
    AAGCACTTCC ATAAGCCTGA GAAAGAGCGA GGCAGTCTGA CGCTGTTGCT CTGTGGAGAG
    TGTGGCCTTG TCTCGGCCTT GGAACAGGCT TTCCAGCATG GATTTAAATC GCCCCGGCTC
    TTCAAAAATG TCTTCATTTG GGATTTCCTG GAAAAAGCAC AAACCTATTA TGAGACATTA
    GAGAAGAATG AAGTAGTCCC TGAGGAAAAC TGGCATACAA GAGCCCGGAA CTTCTGCCGA
    TTTGTCACTG CAATCAACAA TACTCCCCGG AACATCGGCA AGGATGGCAA GTTTCAGATG
    CTGGTGTGCT TGGGAGCCAG GGTTATCATG AAGATTAAAT CTCTTATGTC AGTGCCAGCA
    CATGCTGAGT GTTATGTGAG AGATCACCTC CTACACCACT GGATTGCCCT GCTGGCTGAC
    TGCCCCATCA CTGCACACAT GTATGAGGAT GTGGCACTGA TCAAAGACCA TACACTTGTC
    AATTCCTTGA TTCGTGTGCT GCAGACATTG CAGGAGTTCA ACATCACGCT GGAGACGTCC
    CTTGTCAAGG GCATCGACAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.