MTCL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTCL1
Entrez Gene ID	23255
Full Name	microtubule crosslinking factor 1
Synonyms	CCDC165,KIAA0802,SOGA2
General protein information	Preferred Names microtubule crosslinking factor 1 Names microtubule cross-linking factor 1 PAR-1-interacting protein SOGA family member 2 coiled-coil domain containing 165 coiled-coil domain-containing protein 165
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.22
Disorder MIM:	615766

mRNA and Protein(s)

mRNA	Protein	Name
XM_005258099.4	XP_005258156.1	microtubule cross-linking factor 1 isoform X4
XM_024451111.1	XP_024306879.1	microtubule cross-linking factor 1 isoform X2
XM_011525640.3	XP_011523942.2	microtubule cross-linking factor 1 isoform X4
XM_024451115.1	XP_024306883.1	microtubule cross-linking factor 1 isoform X7
XM_024451119.1	XP_024306887.1	microtubule cross-linking factor 1 isoform X11
XM_024451121.1	XP_024306889.1	microtubule cross-linking factor 1 isoform X15
XM_005258099.5	XP_005258156.2	microtubule cross-linking factor 1 isoform X16
XM_024451124.1	XP_024306892.1	microtubule cross-linking factor 1 isoform X21
XM_024451125.1	XP_024306893.1	microtubule cross-linking factor 1 isoform X22
XM_024451128.1	XP_024306896.1	microtubule cross-linking factor 1 isoform X24
XM_011525640.2	XP_011523942.1	microtubule cross-linking factor 1 isoform X1
XM_017025671.1	XP_016881160.1	microtubule cross-linking factor 1 isoform X5
NM_015210.4	NP_056025.2	microtubule cross-linking factor 1
XM_024451113.1	XP_024306881.1	microtubule cross-linking factor 1 isoform X5
XM_024451114.1	XP_024306882.1	microtubule cross-linking factor 1 isoform X6
XM_024451116.1	XP_024306884.1	microtubule cross-linking factor 1 isoform X8
XM_024451117.1	XP_024306885.1	microtubule cross-linking factor 1 isoform X9
XM_011525641.3	XP_011523943.2	microtubule cross-linking factor 1 isoform X13
XM_024451120.1	XP_024306888.1	microtubule cross-linking factor 1 isoform X14
XM_024451127.1	XP_024306895.1	microtubule cross-linking factor 1 isoform X23
XM_005258098.3	XP_005258155.1	microtubule cross-linking factor 1 isoform X2
XM_024451118.1	XP_024306886.1	microtubule cross-linking factor 1 isoform X10
XM_017025672.1	XP_016881161.1	microtubule cross-linking factor 1 isoform X18
XM_024451122.1	XP_024306890.1	microtubule cross-linking factor 1 isoform X18
XM_017025674.2	XP_016881163.1	microtubule cross-linking factor 1 isoform X18
NM_015210.3	NP_056025.2	microtubule cross-linking factor 1
XM_024451110.1	XP_024306878.1	microtubule cross-linking factor 1 isoform X1
XM_024451123.1	XP_024306891.1	microtubule cross-linking factor 1 isoform X20
XM_024451126.1	XP_024306894.1	microtubule cross-linking factor 1 isoform X22
XM_011525641.2	XP_011523943.1	microtubule cross-linking factor 1 isoform X3
XM_017025674.1	XP_016881163.1	microtubule cross-linking factor 1 isoform X6
XM_024451112.1	XP_024306880.1	microtubule cross-linking factor 1 isoform X3
XM_005258098.4	XP_005258155.2	microtubule cross-linking factor 1 isoform X12
XM_017025671.2	XP_016881160.1	microtubule cross-linking factor 1 isoform X17
XM_017025673.1	XP_016881162.1	microtubule cross-linking factor 1 isoform X18
XM_017025675.1	XP_016881164.1	microtubule cross-linking factor 1 isoform X19

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTCL1	NP_056025.2
Pan troglodytes (chimpanzee)	SOGA2	XP_001140422.2
Canis lupus familiaris (dog)	SOGA2	XP_005623132.1
Bos taurus (cattle)	CCDC165	XP_005224355.1
Danio rerio (zebrafish)	LOC101884972	XP_005171514.1
	LOC706392	XP_001100204.2
Mus musculus (house mouse)	Soga2	NP_766551.3
Rattus norvegicus (Norway rat)	Soga2	XP_006245692.1
Gallus gallus (chicken)	SOGA2	XP_004939798.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular basis of the microtubule-regulating activity of microtubule crosslinking factor 1.
Kader MA, Satake T, Yoshida M, Hayashi I, Suzuki A
PloS one12(8)e0182641(2017)

A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.
Hyodo T, Ito S, Asano-Inami E, Chen D, Senga T
The FEBS journal283(19)3662-3671(2016 10)

MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane.
Sato Y, Hayashi K, Amano Y, Takahashi M, Yonemura S, Hayashi I, Hirose H, Ohno S, Suzuki A
Nature communications55266(2014 Nov)

The novel PAR-1-binding protein MTCL1 has crucial roles in organizing microtubules in polarizing epithelial cells.
Sato Y, Akitsu M, Amano Y, Yamashita K, Ide M, Shimada K, Yamashita A, Hirano H, Arakawa N, Maki T, Hayashi I, Ohno S, Suzuki A
Journal of cell science126(Pt 20)4671-83(2013 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

microtubule-regulating activity of microtubule crosslinking factor 1
Title: Molecular basis of the microtubule-regulating activity of microtubule crosslinking factor 1.

results suggest that the PPP2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL1.
Title: A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.

MTCL1 cooperates with CLASPs and AKAP450/CG-NAP in the formation of the Golgi-derived microtubules
Title: MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane.

Observational study of gene-disease association. (HuGE Navigator)
Title: Replication of a genome-wide case-control study of esophageal squamous cell carcinoma.

The following MTCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104532	XM_024451127.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu104533	XM_024451128.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu104530	XM_024451124.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu104531	XM_024451126.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu104531	XM_024451125.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu104513	XM_024451110.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104514	XM_024451111.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104515	XM_024451112.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104516	XM_011525640.3 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104517	XM_024451113.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104518	XM_024451114.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104519	XM_024451115.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104520	XM_024451116.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104521	XM_024451117.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104522	XM_024451118.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104523	XM_024451119.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104524	XM_005258098.4 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104525	XM_011525641.3 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104526	XM_024451120.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104527	XM_024451121.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104528	XM_005258099.5 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu104529	XM_024451123.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu87828	XM_017025671.2 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87829	XM_024451122.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87829	XM_017025674.2 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87829	XM_017025673.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87829	XM_017025672.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87830	XM_017025675.1 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu27694	NM_015210.4 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu50999	XM_005258098.3 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu51000	XM_005258099.4 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu65813	XM_011525640.2 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu65814	XM_011525641.2 Latest version!	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu27694	NM_015210.3	Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu87829	XM_017025674.1	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu87828	XM_017025671.1	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTCL1 ( XM_024451127.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306895.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X28, mRNA.

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CloneID	OHu104532
Clone ID Related Accession (Same CDS sequence)	XM_024451127.1
Accession Version	XM_024451127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGGAACTACC TTGTTCTGCA 
CTAGCTCCAT CCCTAGAGCC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCCT 
CCGTCCCGTT GGGCCCCACA TTCCCCCACT GCCTCACAGC CTCAGTCACC CGGAGACCCG 
ACGTCCTTGG AGGAGCATGG TGGCGAGGAG CCGCCCGAGG AGCAGCCACA CCGAGATGCA 
AGCTTGCATG GATTATCACA GTATAATTCA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306895.1
CDS	3611..6586
Translation	MEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451127.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451127.1

ORF Insert Sequence:

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGGAACTACC TTGTTCTGCA 
CTAGCTCCAT CCCTAGAGCC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCCT 
CCGTCCCGTT GGGCCCCACA TTCCCCCACT GCCTCACAGC CTCAGTCACC CGGAGACCCG 
ACGTCCTTGG AGGAGCATGG TGGCGAGGAG CCGCCCGAGG AGCAGCCACA CCGAGATGCA 
AGCTTGCATG GATTATCACA GTATAATTCA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451128.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306896.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OHu104533
Clone ID Related Accession (Same CDS sequence)	XM_024451128.1
Accession Version	XM_024451128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA 
GAGAAGACTG AGAACAAGTT GGGAGAACTA GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG 
AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC CTGGCAGACA GTCACAGCCT GGTCATGGAC 
CTGCGCTGGC AGATCCATCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAACTTCTC 
GACCGCCTGG ACAGAGATCG GCAGGAGTGG GAGCGGCAGA AGAAGGAATT CTTGTGGAGG 
ATAGAGCAGT TGCAGAAAGA GAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
AAAGACGGCA ACGTTCGCCC CTTTCCCCAC CAGGGAAGCC TCCGCATGCC CCGTCCAGTG 
GCCATGTGGC CTTGTGCAGA TGCTGACTCC ATCCCGTTTG AAGACCGGCC GCTGTCCAAG 
CTGAAGGAGT CGGACAGGTG CTCGGCCAGT GAGAATCTCT ACCTGGATGC CTTGTCCCTG 
GATGACGAGC CAGAAGAGCC ACCAGCCCAC AGGCCCGAGA GGGAGTTCAG GAACCGCCTC 
CCTGAGGAAG AAGAAAATCA CAAAGGAAAT CTTCAAAGGG CGGTGTCCGT GTCCTCCATG 
TCTGAGTTCC AGCGTCTAAT GGACATCTCC CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC 
ACCAGCAGCA AGGAGGATGT CACCCCACCC CTGTCTCCAG ACGACCTCAA GTACATCGAG 
GAGTTCAACA AGAGCTGGGA CTACACACCC AACAGGGGCC ACAATGGTGG GGGGCCGGAC 
CTTTGGGCCG ACAGGACCGA GGTGGGGCGG GCAGGGCACG AGGACAGCAC AGAGCCTTTC 
CCCGACTCCT CCTGGTACCT AACCACAAGT GTCACCATGA CCACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCACTGCGG AGCCACGTCC TCACCGAGCA GTCGGGGTTG 
CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC AGGGTCGACA GCATCACGGC GGCAGGTGGT 
GAGGGTCCCT TTCCCACAAG CAGAGCCAGA GGGAGCCCGG GAGACACCAA GGGAGGCCCT 
CCAGAACCCA TGCTCAGCAG GTGGCCTTGC ACCTCCCCCA GGCACTCCCG GGACTATGTG 
GAGGGGGCAC GGCGCCCCCT TGATAGTCCC CTCTGTACCT CCCTGGGGTT TGCCTCCCCA 
CTGCACAGCC TGGAGATGTC CAAGAACTTG AGTGATGACA TGAAGGAGGT GGCCTTCTCT 
GTCAGGAATG CCATCTGCTC CGGCCCTGGC GAGCTGCAAG TCAAGGACAT GGCCTGCCAG 
ACCAATGGGT CCCGGACGAT GGGGACCCAG ACTGTTCAGA CCATCAGTGT GGGCTTGCAG 
ACTGAAGCCC TGCGTGGCAG CGGTGTCACC AGCAGCCCCC ACAAGTGTCT CACTCCAAAG 
GCTGGGGGCG GTGCTACACC CGTGTCGTCT CCTTCCCGGA GCCTTAGGAG CAGACAGGTG 
GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAT 
GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC CCCAAAGCCG ACCAGCCAAA TAACAGGACG 
TCACCAGGGA TGGCCCAGAA AGGGTACAGT GAGTCAGCCT GGGCCCGCTC CACCACCACA 
AGGGAGAGCC CCGTGCACAC CACCATTAAT GATGGCCTCT CCAGCCTCTT CAACATCATT 
GACCACAGCC CCGTGGTGCA GGACCCCTTC CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT 
CGCTCAGCAG AGCCCCGACC AGAGCTGGGC CCAGGCCAGG AAACAGGCAC CAATTCCCGA 
GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG TCAGAGATGT GCAGGGAGGA AGGGGGAGAG 
GGCACGCCAG TGAAGCAGGA CTTATCTGCT CCCCCTGGCT ACACGCTCAC TGAGAACGTG 
GCCCGGATCC TCAACAAGAA GCTGCTGGAA CATGCCTTAA AGGAGGAGAG GAGGCAGGCT 
GCCCACGGGC CCCCGGGTCT CCACAGTGAC AGCCACTCGC TGGGGGACAC AGCCGAGCCA 
GGGCCCATGG AGAACCAAAC TGTCTTGCTA ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306896.1
CDS	1054..3804
Translation	MEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_024451128.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451128.1

ORF Insert Sequence:

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA 
GAGAAGACTG AGAACAAGTT GGGAGAACTA GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG 
AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC CTGGCAGACA GTCACAGCCT GGTCATGGAC 
CTGCGCTGGC AGATCCATCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAACTTCTC 
GACCGCCTGG ACAGAGATCG GCAGGAGTGG GAGCGGCAGA AGAAGGAATT CTTGTGGAGG 
ATAGAGCAGT TGCAGAAAGA GAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
AAAGACGGCA ACGTTCGCCC CTTTCCCCAC CAGGGAAGCC TCCGCATGCC CCGTCCAGTG 
GCCATGTGGC CTTGTGCAGA TGCTGACTCC ATCCCGTTTG AAGACCGGCC GCTGTCCAAG 
CTGAAGGAGT CGGACAGGTG CTCGGCCAGT GAGAATCTCT ACCTGGATGC CTTGTCCCTG 
GATGACGAGC CAGAAGAGCC ACCAGCCCAC AGGCCCGAGA GGGAGTTCAG GAACCGCCTC 
CCTGAGGAAG AAGAAAATCA CAAAGGAAAT CTTCAAAGGG CGGTGTCCGT GTCCTCCATG 
TCTGAGTTCC AGCGTCTAAT GGACATCTCC CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC 
ACCAGCAGCA AGGAGGATGT CACCCCACCC CTGTCTCCAG ACGACCTCAA GTACATCGAG 
GAGTTCAACA AGAGCTGGGA CTACACACCC AACAGGGGCC ACAATGGTGG GGGGCCGGAC 
CTTTGGGCCG ACAGGACCGA GGTGGGGCGG GCAGGGCACG AGGACAGCAC AGAGCCTTTC 
CCCGACTCCT CCTGGTACCT AACCACAAGT GTCACCATGA CCACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCACTGCGG AGCCACGTCC TCACCGAGCA GTCGGGGTTG 
CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC AGGGTCGACA GCATCACGGC GGCAGGTGGT 
GAGGGTCCCT TTCCCACAAG CAGAGCCAGA GGGAGCCCGG GAGACACCAA GGGAGGCCCT 
CCAGAACCCA TGCTCAGCAG GTGGCCTTGC ACCTCCCCCA GGCACTCCCG GGACTATGTG 
GAGGGGGCAC GGCGCCCCCT TGATAGTCCC CTCTGTACCT CCCTGGGGTT TGCCTCCCCA 
CTGCACAGCC TGGAGATGTC CAAGAACTTG AGTGATGACA TGAAGGAGGT GGCCTTCTCT 
GTCAGGAATG CCATCTGCTC CGGCCCTGGC GAGCTGCAAG TCAAGGACAT GGCCTGCCAG 
ACCAATGGGT CCCGGACGAT GGGGACCCAG ACTGTTCAGA CCATCAGTGT GGGCTTGCAG 
ACTGAAGCCC TGCGTGGCAG CGGTGTCACC AGCAGCCCCC ACAAGTGTCT CACTCCAAAG 
GCTGGGGGCG GTGCTACACC CGTGTCGTCT CCTTCCCGGA GCCTTAGGAG CAGACAGGTG 
GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAT 
GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC CCCAAAGCCG ACCAGCCAAA TAACAGGACG 
TCACCAGGGA TGGCCCAGAA AGGGTACAGT GAGTCAGCCT GGGCCCGCTC CACCACCACA 
AGGGAGAGCC CCGTGCACAC CACCATTAAT GATGGCCTCT CCAGCCTCTT CAACATCATT 
GACCACAGCC CCGTGGTGCA GGACCCCTTC CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT 
CGCTCAGCAG AGCCCCGACC AGAGCTGGGC CCAGGCCAGG AAACAGGCAC CAATTCCCGA 
GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG TCAGAGATGT GCAGGGAGGA AGGGGGAGAG 
GGCACGCCAG TGAAGCAGGA CTTATCTGCT CCCCCTGGCT ACACGCTCAC TGAGAACGTG 
GCCCGGATCC TCAACAAGAA GCTGCTGGAA CATGCCTTAA AGGAGGAGAG GAGGCAGGCT 
GCCCACGGGC CCCCGGGTCT CCACAGTGAC AGCCACTCGC TGGGGGACAC AGCCGAGCCA 
GGGCCCATGG AGAACCAAAC TGTCTTGCTA ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451124.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306892.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu104530
Clone ID Related Accession (Same CDS sequence)	XM_024451124.1
Accession Version	XM_024451124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGCCTGGAA GCATTGCTGG CACCCCTGCA GCACCTGCCA 
GGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306892.1
CDS	159..3647
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQSLEALLAPLQHLPGM

Target ORF information:

RefSeq Version	XM_024451124.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451124.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGCCTGGAA GCATTGCTGG CACCCCTGCA GCACCTGCCA 
GGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451125.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306893.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu104531
Clone ID Related Accession (Same CDS sequence)	XM_024451125.1 , XM_024451126.1
Accession Version	XM_024451125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGTTGCAGA AAGAGAACAG TCCCCGGAGA GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC 
GGCAACGTTC GCCCCTTTCC CCACCAGGGA AGCCTCCGCA TGCCCCGTCC AGTGGCCATG 
TGGCCTTGTG CAGATGCTGA CTCCATCCCG TTTGAAGACC GGCCGCTGTC CAAGCTGAAG 
GAGTCGGACA GGTGCTCGGC CAGTGAGAAT CTCTACCTGG ATGCCTTGTC CCTGGATGAC 
GAGCCAGAAG AGCCACCAGC CCACAGGCCC GAGAGGGAGT TCAGGAACCG CCTCCCTGAG 
GAAGAAGAAA ATCACAAAGG AAATCTTCAA AGGGCGGTGT CCGTGTCCTC CATGTCTGAG 
TTCCAGCGTC TAATGGACAT CTCCCCCTTC CTGCCTGAGA AGGGCCTGCC GTCCACCAGC 
AGCAAGGAGG ATGTCACCCC ACCCCTGTCT CCAGACGACC TCAAGTACAT CGAGGAGTTC 
AACAAGAGCT GGGACTACAC ACCCAACAGG GGCCACAATG GTGGGGGGCC GGACCTTTGG 
GCCGACAGGA CCGAGGTGGG GCGGGCAGGG CACGAGGACA GCACAGAGCC TTTCCCCGAC 
TCCTCCTGGT ACCTAACCAC AAGTGTCACC ATGACCACGG ACACCATGAC CAGCCCAGAG 
CACTGCCAGA AGCAGCCACT GCGGAGCCAC GTCCTCACCG AGCAGTCGGG GTTGCGCGTG 
TTACACAGCC CGCCTGCCGT GCGCAGGGTC GACAGCATCA CGGCGGCAGG TGGTGAGGGT 
CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC CCGGGAGACA CCAAGGGAGG CCCTCCAGAA 
CCCATGCTCA GCAGGTGGCC TTGCACCTCC CCCAGGCACT CCCGGGACTA TGTGGAGGGG 
GCACGGCGCC CCCTTGATAG TCCCCTCTGT ACCTCCCTGG GGTTTGCCTC CCCACTGCAC 
AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT GACATGAAGG AGGTGGCCTT CTCTGTCAGG 
AATGCCATCT GCTCCGGCCC TGGCGAGCTG CAAGTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGATGGGGAC CCAGACTGTT CAGACCATCA GTGTGGGCTT GCAGACTGAA 
GCCCTGCGTG GCAGCGGTGT CACCAGCAGC CCCCACAAGT GTCTCACTCC AAAGGCTGGG 
GGCGGTGCTA CACCCGTGTC GTCTCCTTCC CGGAGCCTTA GGAGCAGACA GGTGGCCCCT 
GCCATCGAGA AGGTGCAGGC CAAGTTTGAA CGCACATGCT GCTCCCCCAA GTATGGTTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCCGACCAGC CAAATAACAG GACGTCACCA 
GGGATGGCCC AGAAAGGGTA CAGTGAGTCA GCCTGGGCCC GCTCCACCAC CACAAGGGAG 
AGCCCCGTGC ACACCACCAT TAATGATGGC CTCTCCAGCC TCTTCAACAT CATTGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA 
GCAGAGCCCC GACCAGAGCT GGGCCCAGGC CAGGAAACAG GCACCAATTC CCGAGGAAGG 
TCGCCTAGCC CCATTGGGGT GGGGTCAGAG ATGTGCAGGG AGGAAGGGGG AGAGGGCACG 
CCAGTGAAGC AGGACTTATC TGCTCCCCCT GGCTACACGC TCACTGAGAA CGTGGCCCGG 
ATCCTCAACA AGAAGCTGCT GGAACATGCC TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC 
GGGCCCCCGG GTCTCCACAG TGACAGCCAC TCGCTGGGGG ACACAGCCGA GCCAGGGCCC 
ATGGAGGAAC TACCTTGTTC TGCACTAGCT CCATCCCTAG AGCCCTGCTT CTCCAGGCCC 
GAGAGACCAG CAAACCGTCG CCCTCCGTCC CGTTGGGCCC CACATTCCCC CACTGCCTCA 
CAGCCTCAGT CACCCGGAGA CCCGACGTCC TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC 
GAGGAGCAGC CACACCGAGA TGCAAGCTTG CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306893.1
CDS	3734..6793
Translation	MEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451125.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451125.1

ORF Insert Sequence:

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGTTGCAGA AAGAGAACAG TCCCCGGAGA GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC 
GGCAACGTTC GCCCCTTTCC CCACCAGGGA AGCCTCCGCA TGCCCCGTCC AGTGGCCATG 
TGGCCTTGTG CAGATGCTGA CTCCATCCCG TTTGAAGACC GGCCGCTGTC CAAGCTGAAG 
GAGTCGGACA GGTGCTCGGC CAGTGAGAAT CTCTACCTGG ATGCCTTGTC CCTGGATGAC 
GAGCCAGAAG AGCCACCAGC CCACAGGCCC GAGAGGGAGT TCAGGAACCG CCTCCCTGAG 
GAAGAAGAAA ATCACAAAGG AAATCTTCAA AGGGCGGTGT CCGTGTCCTC CATGTCTGAG 
TTCCAGCGTC TAATGGACAT CTCCCCCTTC CTGCCTGAGA AGGGCCTGCC GTCCACCAGC 
AGCAAGGAGG ATGTCACCCC ACCCCTGTCT CCAGACGACC TCAAGTACAT CGAGGAGTTC 
AACAAGAGCT GGGACTACAC ACCCAACAGG GGCCACAATG GTGGGGGGCC GGACCTTTGG 
GCCGACAGGA CCGAGGTGGG GCGGGCAGGG CACGAGGACA GCACAGAGCC TTTCCCCGAC 
TCCTCCTGGT ACCTAACCAC AAGTGTCACC ATGACCACGG ACACCATGAC CAGCCCAGAG 
CACTGCCAGA AGCAGCCACT GCGGAGCCAC GTCCTCACCG AGCAGTCGGG GTTGCGCGTG 
TTACACAGCC CGCCTGCCGT GCGCAGGGTC GACAGCATCA CGGCGGCAGG TGGTGAGGGT 
CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC CCGGGAGACA CCAAGGGAGG CCCTCCAGAA 
CCCATGCTCA GCAGGTGGCC TTGCACCTCC CCCAGGCACT CCCGGGACTA TGTGGAGGGG 
GCACGGCGCC CCCTTGATAG TCCCCTCTGT ACCTCCCTGG GGTTTGCCTC CCCACTGCAC 
AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT GACATGAAGG AGGTGGCCTT CTCTGTCAGG 
AATGCCATCT GCTCCGGCCC TGGCGAGCTG CAAGTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGATGGGGAC CCAGACTGTT CAGACCATCA GTGTGGGCTT GCAGACTGAA 
GCCCTGCGTG GCAGCGGTGT CACCAGCAGC CCCCACAAGT GTCTCACTCC AAAGGCTGGG 
GGCGGTGCTA CACCCGTGTC GTCTCCTTCC CGGAGCCTTA GGAGCAGACA GGTGGCCCCT 
GCCATCGAGA AGGTGCAGGC CAAGTTTGAA CGCACATGCT GCTCCCCCAA GTATGGTTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCCGACCAGC CAAATAACAG GACGTCACCA 
GGGATGGCCC AGAAAGGGTA CAGTGAGTCA GCCTGGGCCC GCTCCACCAC CACAAGGGAG 
AGCCCCGTGC ACACCACCAT TAATGATGGC CTCTCCAGCC TCTTCAACAT CATTGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA 
GCAGAGCCCC GACCAGAGCT GGGCCCAGGC CAGGAAACAG GCACCAATTC CCGAGGAAGG 
TCGCCTAGCC CCATTGGGGT GGGGTCAGAG ATGTGCAGGG AGGAAGGGGG AGAGGGCACG 
CCAGTGAAGC AGGACTTATC TGCTCCCCCT GGCTACACGC TCACTGAGAA CGTGGCCCGG 
ATCCTCAACA AGAAGCTGCT GGAACATGCC TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC 
GGGCCCCCGG GTCTCCACAG TGACAGCCAC TCGCTGGGGG ACACAGCCGA GCCAGGGCCC 
ATGGAGGAAC TACCTTGTTC TGCACTAGCT CCATCCCTAG AGCCCTGCTT CTCCAGGCCC 
GAGAGACCAG CAAACCGTCG CCCTCCGTCC CGTTGGGCCC CACATTCCCC CACTGCCTCA 
CAGCCTCAGT CACCCGGAGA CCCGACGTCC TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC 
GAGGAGCAGC CACACCGAGA TGCAAGCTTG CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451126.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306894.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu104531
Clone ID Related Accession (Same CDS sequence)	XM_024451125.1 , XM_024451126.1
Accession Version	XM_024451126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGTTGCAGA AAGAGAACAG TCCCCGGAGA GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC 
GGCAACGTTC GCCCCTTTCC CCACCAGGGA AGCCTCCGCA TGCCCCGTCC AGTGGCCATG 
TGGCCTTGTG CAGATGCTGA CTCCATCCCG TTTGAAGACC GGCCGCTGTC CAAGCTGAAG 
GAGTCGGACA GGTGCTCGGC CAGTGAGAAT CTCTACCTGG ATGCCTTGTC CCTGGATGAC 
GAGCCAGAAG AGCCACCAGC CCACAGGCCC GAGAGGGAGT TCAGGAACCG CCTCCCTGAG 
GAAGAAGAAA ATCACAAAGG AAATCTTCAA AGGGCGGTGT CCGTGTCCTC CATGTCTGAG 
TTCCAGCGTC TAATGGACAT CTCCCCCTTC CTGCCTGAGA AGGGCCTGCC GTCCACCAGC 
AGCAAGGAGG ATGTCACCCC ACCCCTGTCT CCAGACGACC TCAAGTACAT CGAGGAGTTC 
AACAAGAGCT GGGACTACAC ACCCAACAGG GGCCACAATG GTGGGGGGCC GGACCTTTGG 
GCCGACAGGA CCGAGGTGGG GCGGGCAGGG CACGAGGACA GCACAGAGCC TTTCCCCGAC 
TCCTCCTGGT ACCTAACCAC AAGTGTCACC ATGACCACGG ACACCATGAC CAGCCCAGAG 
CACTGCCAGA AGCAGCCACT GCGGAGCCAC GTCCTCACCG AGCAGTCGGG GTTGCGCGTG 
TTACACAGCC CGCCTGCCGT GCGCAGGGTC GACAGCATCA CGGCGGCAGG TGGTGAGGGT 
CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC CCGGGAGACA CCAAGGGAGG CCCTCCAGAA 
CCCATGCTCA GCAGGTGGCC TTGCACCTCC CCCAGGCACT CCCGGGACTA TGTGGAGGGG 
GCACGGCGCC CCCTTGATAG TCCCCTCTGT ACCTCCCTGG GGTTTGCCTC CCCACTGCAC 
AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT GACATGAAGG AGGTGGCCTT CTCTGTCAGG 
AATGCCATCT GCTCCGGCCC TGGCGAGCTG CAAGTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGATGGGGAC CCAGACTGTT CAGACCATCA GTGTGGGCTT GCAGACTGAA 
GCCCTGCGTG GCAGCGGTGT CACCAGCAGC CCCCACAAGT GTCTCACTCC AAAGGCTGGG 
GGCGGTGCTA CACCCGTGTC GTCTCCTTCC CGGAGCCTTA GGAGCAGACA GGTGGCCCCT 
GCCATCGAGA AGGTGCAGGC CAAGTTTGAA CGCACATGCT GCTCCCCCAA GTATGGTTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCCGACCAGC CAAATAACAG GACGTCACCA 
GGGATGGCCC AGAAAGGGTA CAGTGAGTCA GCCTGGGCCC GCTCCACCAC CACAAGGGAG 
AGCCCCGTGC ACACCACCAT TAATGATGGC CTCTCCAGCC TCTTCAACAT CATTGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA 
GCAGAGCCCC GACCAGAGCT GGGCCCAGGC CAGGAAACAG GCACCAATTC CCGAGGAAGG 
TCGCCTAGCC CCATTGGGGT GGGGTCAGAG ATGTGCAGGG AGGAAGGGGG AGAGGGCACG 
CCAGTGAAGC AGGACTTATC TGCTCCCCCT GGCTACACGC TCACTGAGAA CGTGGCCCGG 
ATCCTCAACA AGAAGCTGCT GGAACATGCC TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC 
GGGCCCCCGG GTCTCCACAG TGACAGCCAC TCGCTGGGGG ACACAGCCGA GCCAGGGCCC 
ATGGAGGAAC TACCTTGTTC TGCACTAGCT CCATCCCTAG AGCCCTGCTT CTCCAGGCCC 
GAGAGACCAG CAAACCGTCG CCCTCCGTCC CGTTGGGCCC CACATTCCCC CACTGCCTCA 
CAGCCTCAGT CACCCGGAGA CCCGACGTCC TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC 
GAGGAGCAGC CACACCGAGA TGCAAGCTTG CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306894.1
CDS	3611..6670
Translation	MEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451126.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451126.1

ORF Insert Sequence:

ATGGAGGAGG AGCACCTCTA TGCCTTGAGG TGGAAAGAAC TGGAAATGCA CAGCCTGGCT 
TTGCAAAACA CCCTCCATGA GCGAACCTGG AGTGATGAGA AGAATCTGAT GCAGCAGGAG 
CTCCGGTCCT TGAAGCAGAA CATTTTCCTC TTCTACGTCA AACTCAGGTG GCTGCTGAAA 
CACTGGCGGC AAGGGAAGCA GATGGAGGAG GAAGGAGAGG AGTTCACTGA GGGTGAACAT 
CCAGAGACCC TCTCCAGGCT CGGGGAGCTT GGAGTCCAGG GGGGTCACCA GGCGGATGGC 
CCAGACCACG ACAGTGACCG AGGCTGTGGC TTTCCAGTGG GGGAGCACTC CCCACACTCC 
CGGGTGCAGA TTGGAGATCA CAGCTTGCGG CTGCAGACCG CGGACAGGGG ACAGCCCCAC 
AAACAGGTGG TGGAAAACCA GCAGCTGTTC AGCGCCTTCA AGGCCTTGCT GGAGGACTTC 
CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC CGACTACGGC TGCAGCAGCA ATATGCCAGC 
GACAAGGCGG CCTGGGACGT GGAGTGGGCC GTGCTCAAGT GCCGTCTGGA ACAGAATTGT 
TGTGGATATC CCAGAATTAA CATTGAGGAG GAGACTTTAG GCTTCACCAG GCTGCCAGCT 
GGGTCCACGG TAAAAACGTT GAAGAGCCTT GGGTTGCAGA GATTGGAGCT GGAAGAGAAG 
ACTGAGAACA AGTTGGGAGA ACTAGGCTCC TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG 
GAGAGAGAGG TGCACCAGAA GCTCCTGGCA GACAGTCACA GCCTGGTCAT GGACCTGCGC 
TGGCAGATCC ATCACAGCGA GAAGAACTGG AACCGGGAGA AGGTGGAACT TCTCGACCGC 
CTGGACAGAG ATCGGCAGGA GTGGGAGCGG CAGAAGAAGG AATTCTTGTG GAGGATAGAG 
CAGTTGCAGA AAGAGAACAG TCCCCGGAGA GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC 
GGCAACGTTC GCCCCTTTCC CCACCAGGGA AGCCTCCGCA TGCCCCGTCC AGTGGCCATG 
TGGCCTTGTG CAGATGCTGA CTCCATCCCG TTTGAAGACC GGCCGCTGTC CAAGCTGAAG 
GAGTCGGACA GGTGCTCGGC CAGTGAGAAT CTCTACCTGG ATGCCTTGTC CCTGGATGAC 
GAGCCAGAAG AGCCACCAGC CCACAGGCCC GAGAGGGAGT TCAGGAACCG CCTCCCTGAG 
GAAGAAGAAA ATCACAAAGG AAATCTTCAA AGGGCGGTGT CCGTGTCCTC CATGTCTGAG 
TTCCAGCGTC TAATGGACAT CTCCCCCTTC CTGCCTGAGA AGGGCCTGCC GTCCACCAGC 
AGCAAGGAGG ATGTCACCCC ACCCCTGTCT CCAGACGACC TCAAGTACAT CGAGGAGTTC 
AACAAGAGCT GGGACTACAC ACCCAACAGG GGCCACAATG GTGGGGGGCC GGACCTTTGG 
GCCGACAGGA CCGAGGTGGG GCGGGCAGGG CACGAGGACA GCACAGAGCC TTTCCCCGAC 
TCCTCCTGGT ACCTAACCAC AAGTGTCACC ATGACCACGG ACACCATGAC CAGCCCAGAG 
CACTGCCAGA AGCAGCCACT GCGGAGCCAC GTCCTCACCG AGCAGTCGGG GTTGCGCGTG 
TTACACAGCC CGCCTGCCGT GCGCAGGGTC GACAGCATCA CGGCGGCAGG TGGTGAGGGT 
CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC CCGGGAGACA CCAAGGGAGG CCCTCCAGAA 
CCCATGCTCA GCAGGTGGCC TTGCACCTCC CCCAGGCACT CCCGGGACTA TGTGGAGGGG 
GCACGGCGCC CCCTTGATAG TCCCCTCTGT ACCTCCCTGG GGTTTGCCTC CCCACTGCAC 
AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT GACATGAAGG AGGTGGCCTT CTCTGTCAGG 
AATGCCATCT GCTCCGGCCC TGGCGAGCTG CAAGTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGATGGGGAC CCAGACTGTT CAGACCATCA GTGTGGGCTT GCAGACTGAA 
GCCCTGCGTG GCAGCGGTGT CACCAGCAGC CCCCACAAGT GTCTCACTCC AAAGGCTGGG 
GGCGGTGCTA CACCCGTGTC GTCTCCTTCC CGGAGCCTTA GGAGCAGACA GGTGGCCCCT 
GCCATCGAGA AGGTGCAGGC CAAGTTTGAA CGCACATGCT GCTCCCCCAA GTATGGTTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCCGACCAGC CAAATAACAG GACGTCACCA 
GGGATGGCCC AGAAAGGGTA CAGTGAGTCA GCCTGGGCCC GCTCCACCAC CACAAGGGAG 
AGCCCCGTGC ACACCACCAT TAATGATGGC CTCTCCAGCC TCTTCAACAT CATTGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA 
GCAGAGCCCC GACCAGAGCT GGGCCCAGGC CAGGAAACAG GCACCAATTC CCGAGGAAGG 
TCGCCTAGCC CCATTGGGGT GGGGTCAGAG ATGTGCAGGG AGGAAGGGGG AGAGGGCACG 
CCAGTGAAGC AGGACTTATC TGCTCCCCCT GGCTACACGC TCACTGAGAA CGTGGCCCGG 
ATCCTCAACA AGAAGCTGCT GGAACATGCC TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC 
GGGCCCCCGG GTCTCCACAG TGACAGCCAC TCGCTGGGGG ACACAGCCGA GCCAGGGCCC 
ATGGAGGAAC TACCTTGTTC TGCACTAGCT CCATCCCTAG AGCCCTGCTT CTCCAGGCCC 
GAGAGACCAG CAAACCGTCG CCCTCCGTCC CGTTGGGCCC CACATTCCCC CACTGCCTCA 
CAGCCTCAGT CACCCGGAGA CCCGACGTCC TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC 
GAGGAGCAGC CACACCGAGA TGCAAGCTTG CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451110.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306878.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

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CloneID	OHu104513
Clone ID Related Accession (Same CDS sequence)	XM_024451110.1
Accession Version	XM_024451110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGGAA 
CTACCTTGTT CTGCACTAGC TCCATCCCTA GAGCCCTGCT TCTCCAGGCC CGAGAGACCA 
GCAAACCGTC GCCCTCCGTC CCGTTGGGCC CCACATTCCC CCACTGCCTC ACAGCCTCAG 
TCACCCGGAG ACCCGACGTC CTTGGAGGAG CATGGTGGCG AGGAGCCGCC CGAGGAGCAG 
CCACACCGAG ATGCAAGCTT GCATGGATTA TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306878.1
CDS	159..5369
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451110.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451110.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGGAA 
CTACCTTGTT CTGCACTAGC TCCATCCCTA GAGCCCTGCT TCTCCAGGCC CGAGAGACCA 
GCAAACCGTC GCCCTCCGTC CCGTTGGGCC CCACATTCCC CCACTGCCTC ACAGCCTCAG 
TCACCCGGAG ACCCGACGTC CTTGGAGGAG CATGGTGGCG AGGAGCCGCC CGAGGAGCAG 
CCACACCGAG ATGCAAGCTT GCATGGATTA TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451111.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306879.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

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CloneID	OHu104514
Clone ID Related Accession (Same CDS sequence)	XM_024451111.1
Accession Version	XM_024451111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306879.1
CDS	159..5366
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451111.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451112.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306880.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

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CloneID	OHu104515
Clone ID Related Accession (Same CDS sequence)	XM_024451112.1
Accession Version	XM_024451112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG CTCAGGGGCC CCCCCGTTTT ACCTGAGCAG 
AGTGTATCCA TAGAGGAGCT ACAGGGTCAG CTCGTGCAGG CGGCCAGACT GCATCAAGAG 
GAGACAGAGA CATTTACAAA CAAGATCCAT AAGATGGAGG AGGAGCACCT CTATGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAAA ACACCCTCCA TGAGCGAACC 
TGGAGTGATG AGAAGAATCT GATGCAGCAG GAGCTCCGGT CCTTGAAGCA GAACATTTTC 
CTCTTCTACG TCAAACTCAG GTGGCTGCTG AAACACTGGC GGCAAGGGAA GCAGATGGAG 
GAGGAAGGAG AGGAGTTCAC TGAGGGTGAA CATCCAGAGA CCCTCTCCAG GCTCGGGGAG 
CTTGGAGTCC AGGGGGGTCA CCAGGCGGAT GGCCCAGACC ACGACAGTGA CCGAGGCTGT 
GGCTTTCCAG TGGGGGAGCA CTCCCCACAC TCCCGGGTGC AGATTGGAGA TCACAGCTTG 
CGGCTGCAGA CCGCGGACAG GGGACAGCCC CACAAACAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGCGCCT TCAAGGCCTT GCTGGAGGAC TTCCGTGCGG AGCTGCGGGA GGATGAGCGT 
GCCCGACTAC GGCTGCAGCA GCAATATGCC AGCGACAAGG CGGCCTGGGA CGTGGAGTGG 
GCCGTGCTCA AGTGCCGTCT GGAACAGAAT TGTTGTGGAT ATCCCAGAAT TAACATTGAG 
GAGGAGACTT TAGGCTTCAC CAGGCTGCCA GCTGGGTCCA CGGTAAAAAC GTTGAAGAGC 
CTTGGGTTGC AGAGATTGGA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306880.1
CDS	159..5291
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451112.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG CTCAGGGGCC CCCCCGTTTT ACCTGAGCAG 
AGTGTATCCA TAGAGGAGCT ACAGGGTCAG CTCGTGCAGG CGGCCAGACT GCATCAAGAG 
GAGACAGAGA CATTTACAAA CAAGATCCAT AAGATGGAGG AGGAGCACCT CTATGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAAA ACACCCTCCA TGAGCGAACC 
TGGAGTGATG AGAAGAATCT GATGCAGCAG GAGCTCCGGT CCTTGAAGCA GAACATTTTC 
CTCTTCTACG TCAAACTCAG GTGGCTGCTG AAACACTGGC GGCAAGGGAA GCAGATGGAG 
GAGGAAGGAG AGGAGTTCAC TGAGGGTGAA CATCCAGAGA CCCTCTCCAG GCTCGGGGAG 
CTTGGAGTCC AGGGGGGTCA CCAGGCGGAT GGCCCAGACC ACGACAGTGA CCGAGGCTGT 
GGCTTTCCAG TGGGGGAGCA CTCCCCACAC TCCCGGGTGC AGATTGGAGA TCACAGCTTG 
CGGCTGCAGA CCGCGGACAG GGGACAGCCC CACAAACAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGCGCCT TCAAGGCCTT GCTGGAGGAC TTCCGTGCGG AGCTGCGGGA GGATGAGCGT 
GCCCGACTAC GGCTGCAGCA GCAATATGCC AGCGACAAGG CGGCCTGGGA CGTGGAGTGG 
GCCGTGCTCA AGTGCCGTCT GGAACAGAAT TGTTGTGGAT ATCCCAGAAT TAACATTGAG 
GAGGAGACTT TAGGCTTCAC CAGGCTGCCA GCTGGGTCCA CGGTAAAAAC GTTGAAGAGC 
CTTGGGTTGC AGAGATTGGA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_011525640.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523942.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

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CloneID	OHu104516
Clone ID Related Accession (Same CDS sequence)	XM_011525640.3
Accession Version	XM_011525640.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGGGAAGC 
CTCCGCATGC CCCGTCCAGT GGCCATGTGG CCTTGTGCAG ATGCTGACTC CATCCCGTTT 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAG TCGGACAGGT GCTCGGCCAG TGAGAATCTC 
TACCTGGATG CCTTGTCCCT GGATGACGAG CCAGAAGAGC CACCAGCCCA CAGGCCCGAG 
AGGGAGTTCA GGAACCGCCT CCCTGAGGAA GAAGAAAATC ACAAAGGAAA TCTTCAAAGG 
GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC CAGCGTCTAA TGGACATCTC CCCCTTCCTG 
CCTGAGAAGG GCCTGCCGTC CACCAGCAGC AAGGAGGATG TCACCCCACC CCTGTCTCCA 
GACGACCTCA AGTACATCGA GGAGTTCAAC AAGAGCTGGG ACTACACACC CAACAGGGGC 
CACAATGGTG GGGGGCCGGA CCTTTGGGCC GACAGGACCG AGGTGGGGCG GGCAGGGCAC 
GAGGACAGCA CAGAGCCTTT CCCCGACTCC TCCTGGTACC TAACCACAAG TGTCACCATG 
ACCACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCACTGCG GAGCCACGTC 
CTCACCGAGC AGTCGGGGTT GCGCGTGTTA CACAGCCCGC CTGCCGTGCG CAGGGTCGAC 
AGCATCACGG CGGCAGGTGG TGAGGGTCCC TTTCCCACAA GCAGAGCCAG AGGGAGCCCG 
GGAGACACCA AGGGAGGCCC TCCAGAACCC ATGCTCAGCA GGTGGCCTTG CACCTCCCCC 
AGGCACTCCC GGGACTATGT GGAGGGGGCA CGGCGCCCCC TTGATAGTCC CCTCTGTACC 
TCCCTGGGGT TTGCCTCCCC ACTGCACAGC CTGGAGATGT CCAAGAACTT GAGTGATGAC 
ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT GCCATCTGCT CCGGCCCTGG CGAGCTGCAA 
GTCAAGGACA TGGCCTGCCA GACCAATGGG TCCCGGACGA TGGGGACCCA GACTGTTCAG 
ACCATCAGTG TGGGCTTGCA GACTGAAGCC CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC 
CACAAGTGTC TCACTCCAAA GGCTGGGGGC GGTGCTACAC CCGTGTCGTC TCCTTCCCGG 
AGCCTTAGGA GCAGACAGGT GGCCCCTGCC ATCGAGAAGG TGCAGGCCAA GTTTGAACGC 
ACATGCTGCT CCCCCAAGTA TGGTTCTCCC AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC 
GACCAGCCAA ATAACAGGAC GTCACCAGGG ATGGCCCAGA AAGGGTACAG TGAGTCAGCC 
TGGGCCCGCT CCACCACCAC AAGGGAGAGC CCCGTGCACA CCACCATTAA TGATGGCCTC 
TCCAGCCTCT TCAACATCAT TGACCACAGC CCCGTGGTGC AGGACCCCTT CCAGAAGGGG 
CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG 
GAAACAGGCA CCAATTCCCG AGGAAGGTCG CCTAGCCCCA TTGGGGTGGG GTCAGAGATG 
TGCAGGGAGG AAGGGGGAGA GGGCACGCCA GTGAAGCAGG ACTTATCTGC TCCCCCTGGC 
TACACGCTCA CTGAGAACGT GGCCCGGATC CTCAACAAGA AGCTGCTGGA ACATGCCTTA 
AAGGAGGAGA GGAGGCAGGC TGCCCACGGG CCCCCGGGTC TCCACAGTGA CAGCCACTCG 
CTGGGGGACA CAGCCGAGCC AGGGCCCATG GAGGAACTAC CTTGTTCTGC ACTAGCTCCA 
TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGT 
TGGGCCCCAC ATTCCCCCAC TGCCTCACAG CCTCAGTCAC CCGGAGACCC GACGTCCTTG 
GAGGAGCATG GTGGCGAGGA GCCGCCCGAG GAGCAGCCAC ACCGAGATGC AAGCTTGCAT 
GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523942.2
CDS	159..5285
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_011525640.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525640.3

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGGGAAGC 
CTCCGCATGC CCCGTCCAGT GGCCATGTGG CCTTGTGCAG ATGCTGACTC CATCCCGTTT 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAG TCGGACAGGT GCTCGGCCAG TGAGAATCTC 
TACCTGGATG CCTTGTCCCT GGATGACGAG CCAGAAGAGC CACCAGCCCA CAGGCCCGAG 
AGGGAGTTCA GGAACCGCCT CCCTGAGGAA GAAGAAAATC ACAAAGGAAA TCTTCAAAGG 
GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC CAGCGTCTAA TGGACATCTC CCCCTTCCTG 
CCTGAGAAGG GCCTGCCGTC CACCAGCAGC AAGGAGGATG TCACCCCACC CCTGTCTCCA 
GACGACCTCA AGTACATCGA GGAGTTCAAC AAGAGCTGGG ACTACACACC CAACAGGGGC 
CACAATGGTG GGGGGCCGGA CCTTTGGGCC GACAGGACCG AGGTGGGGCG GGCAGGGCAC 
GAGGACAGCA CAGAGCCTTT CCCCGACTCC TCCTGGTACC TAACCACAAG TGTCACCATG 
ACCACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCACTGCG GAGCCACGTC 
CTCACCGAGC AGTCGGGGTT GCGCGTGTTA CACAGCCCGC CTGCCGTGCG CAGGGTCGAC 
AGCATCACGG CGGCAGGTGG TGAGGGTCCC TTTCCCACAA GCAGAGCCAG AGGGAGCCCG 
GGAGACACCA AGGGAGGCCC TCCAGAACCC ATGCTCAGCA GGTGGCCTTG CACCTCCCCC 
AGGCACTCCC GGGACTATGT GGAGGGGGCA CGGCGCCCCC TTGATAGTCC CCTCTGTACC 
TCCCTGGGGT TTGCCTCCCC ACTGCACAGC CTGGAGATGT CCAAGAACTT GAGTGATGAC 
ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT GCCATCTGCT CCGGCCCTGG CGAGCTGCAA 
GTCAAGGACA TGGCCTGCCA GACCAATGGG TCCCGGACGA TGGGGACCCA GACTGTTCAG 
ACCATCAGTG TGGGCTTGCA GACTGAAGCC CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC 
CACAAGTGTC TCACTCCAAA GGCTGGGGGC GGTGCTACAC CCGTGTCGTC TCCTTCCCGG 
AGCCTTAGGA GCAGACAGGT GGCCCCTGCC ATCGAGAAGG TGCAGGCCAA GTTTGAACGC 
ACATGCTGCT CCCCCAAGTA TGGTTCTCCC AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC 
GACCAGCCAA ATAACAGGAC GTCACCAGGG ATGGCCCAGA AAGGGTACAG TGAGTCAGCC 
TGGGCCCGCT CCACCACCAC AAGGGAGAGC CCCGTGCACA CCACCATTAA TGATGGCCTC 
TCCAGCCTCT TCAACATCAT TGACCACAGC CCCGTGGTGC AGGACCCCTT CCAGAAGGGG 
CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG 
GAAACAGGCA CCAATTCCCG AGGAAGGTCG CCTAGCCCCA TTGGGGTGGG GTCAGAGATG 
TGCAGGGAGG AAGGGGGAGA GGGCACGCCA GTGAAGCAGG ACTTATCTGC TCCCCCTGGC 
TACACGCTCA CTGAGAACGT GGCCCGGATC CTCAACAAGA AGCTGCTGGA ACATGCCTTA 
AAGGAGGAGA GGAGGCAGGC TGCCCACGGG CCCCCGGGTC TCCACAGTGA CAGCCACTCG 
CTGGGGGACA CAGCCGAGCC AGGGCCCATG GAGGAACTAC CTTGTTCTGC ACTAGCTCCA 
TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGT 
TGGGCCCCAC ATTCCCCCAC TGCCTCACAG CCTCAGTCAC CCGGAGACCC GACGTCCTTG 
GAGGAGCATG GTGGCGAGGA GCCGCCCGAG GAGCAGCCAC ACCGAGATGC AAGCTTGCAT 
GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451113.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306881.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu104517
Clone ID Related Accession (Same CDS sequence)	XM_024451113.1
Accession Version	XM_024451113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
TTGCAGAAAG AGAACAGTCC CCGGAGAGGT GGCAGTTTCC TCTGTGATCA AAAAGACGGC 
AACGTTCGCC CCTTTCCCCA CCAGGGAAGC CTCCGCATGC CCCGTCCAGT GGCCATGTGG 
CCTTGTGCAG ATGCTGACTC CATCCCGTTT GAAGACCGGC CGCTGTCCAA GCTGAAGGAG 
TCGGACAGGT GCTCGGCCAG TGAGAATCTC TACCTGGATG CCTTGTCCCT GGATGACGAG 
CCAGAAGAGC CACCAGCCCA CAGGCCCGAG AGGGAGTTCA GGAACCGCCT CCCTGAGGAA 
GAAGAAAATC ACAAAGGAAA TCTTCAAAGG GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTAA TGGACATCTC CCCCTTCCTG CCTGAGAAGG GCCTGCCGTC CACCAGCAGC 
AAGGAGGATG TCACCCCACC CCTGTCTCCA GACGACCTCA AGTACATCGA GGAGTTCAAC 
AAGAGCTGGG ACTACACACC CAACAGGGGC CACAATGGTG GGGGGCCGGA CCTTTGGGCC 
GACAGGACCG AGGTGGGGCG GGCAGGGCAC GAGGACAGCA CAGAGCCTTT CCCCGACTCC 
TCCTGGTACC TAACCACAAG TGTCACCATG ACCACGGACA CCATGACCAG CCCAGAGCAC 
TGCCAGAAGC AGCCACTGCG GAGCCACGTC CTCACCGAGC AGTCGGGGTT GCGCGTGTTA 
CACAGCCCGC CTGCCGTGCG CAGGGTCGAC AGCATCACGG CGGCAGGTGG TGAGGGTCCC 
TTTCCCACAA GCAGAGCCAG AGGGAGCCCG GGAGACACCA AGGGAGGCCC TCCAGAACCC 
ATGCTCAGCA GGTGGCCTTG CACCTCCCCC AGGCACTCCC GGGACTATGT GGAGGGGGCA 
CGGCGCCCCC TTGATAGTCC CCTCTGTACC TCCCTGGGGT TTGCCTCCCC ACTGCACAGC 
CTGGAGATGT CCAAGAACTT GAGTGATGAC ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT 
GCCATCTGCT CCGGCCCTGG CGAGCTGCAA GTCAAGGACA TGGCCTGCCA GACCAATGGG 
TCCCGGACGA TGGGGACCCA GACTGTTCAG ACCATCAGTG TGGGCTTGCA GACTGAAGCC 
CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC CACAAGTGTC TCACTCCAAA GGCTGGGGGC 
GGTGCTACAC CCGTGTCGTC TCCTTCCCGG AGCCTTAGGA GCAGACAGGT GGCCCCTGCC 
ATCGAGAAGG TGCAGGCCAA GTTTGAACGC ACATGCTGCT CCCCCAAGTA TGGTTCTCCC 
AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC GACCAGCCAA ATAACAGGAC GTCACCAGGG 
ATGGCCCAGA AAGGGTACAG TGAGTCAGCC TGGGCCCGCT CCACCACCAC AAGGGAGAGC 
CCCGTGCACA CCACCATTAA TGATGGCCTC TCCAGCCTCT TCAACATCAT TGACCACAGC 
CCCGTGGTGC AGGACCCCTT CCAGAAGGGG CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA 
GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG GAAACAGGCA CCAATTCCCG AGGAAGGTCG 
CCTAGCCCCA TTGGGGTGGG GTCAGAGATG TGCAGGGAGG AAGGGGGAGA GGGCACGCCA 
GTGAAGCAGG ACTTATCTGC TCCCCCTGGC TACACGCTCA CTGAGAACGT GGCCCGGATC 
CTCAACAAGA AGCTGCTGGA ACATGCCTTA AAGGAGGAGA GGAGGCAGGC TGCCCACGGG 
CCCCCGGGTC TCCACAGTGA CAGCCACTCG CTGGGGGACA CAGCCGAGCC AGGGCCCATG 
GAGGAACTAC CTTGTTCTGC ACTAGCTCCA TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG 
AGACCAGCAA ACCGTCGCCC TCCGTCCCGT TGGGCCCCAC ATTCCCCCAC TGCCTCACAG 
CCTCAGTCAC CCGGAGACCC GACGTCCTTG GAGGAGCATG GTGGCGAGGA GCCGCCCGAG 
GAGCAGCCAC ACCGAGATGC AAGCTTGCAT GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306881.1
CDS	159..5255
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451113.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451113.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
TTGCAGAAAG AGAACAGTCC CCGGAGAGGT GGCAGTTTCC TCTGTGATCA AAAAGACGGC 
AACGTTCGCC CCTTTCCCCA CCAGGGAAGC CTCCGCATGC CCCGTCCAGT GGCCATGTGG 
CCTTGTGCAG ATGCTGACTC CATCCCGTTT GAAGACCGGC CGCTGTCCAA GCTGAAGGAG 
TCGGACAGGT GCTCGGCCAG TGAGAATCTC TACCTGGATG CCTTGTCCCT GGATGACGAG 
CCAGAAGAGC CACCAGCCCA CAGGCCCGAG AGGGAGTTCA GGAACCGCCT CCCTGAGGAA 
GAAGAAAATC ACAAAGGAAA TCTTCAAAGG GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTAA TGGACATCTC CCCCTTCCTG CCTGAGAAGG GCCTGCCGTC CACCAGCAGC 
AAGGAGGATG TCACCCCACC CCTGTCTCCA GACGACCTCA AGTACATCGA GGAGTTCAAC 
AAGAGCTGGG ACTACACACC CAACAGGGGC CACAATGGTG GGGGGCCGGA CCTTTGGGCC 
GACAGGACCG AGGTGGGGCG GGCAGGGCAC GAGGACAGCA CAGAGCCTTT CCCCGACTCC 
TCCTGGTACC TAACCACAAG TGTCACCATG ACCACGGACA CCATGACCAG CCCAGAGCAC 
TGCCAGAAGC AGCCACTGCG GAGCCACGTC CTCACCGAGC AGTCGGGGTT GCGCGTGTTA 
CACAGCCCGC CTGCCGTGCG CAGGGTCGAC AGCATCACGG CGGCAGGTGG TGAGGGTCCC 
TTTCCCACAA GCAGAGCCAG AGGGAGCCCG GGAGACACCA AGGGAGGCCC TCCAGAACCC 
ATGCTCAGCA GGTGGCCTTG CACCTCCCCC AGGCACTCCC GGGACTATGT GGAGGGGGCA 
CGGCGCCCCC TTGATAGTCC CCTCTGTACC TCCCTGGGGT TTGCCTCCCC ACTGCACAGC 
CTGGAGATGT CCAAGAACTT GAGTGATGAC ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT 
GCCATCTGCT CCGGCCCTGG CGAGCTGCAA GTCAAGGACA TGGCCTGCCA GACCAATGGG 
TCCCGGACGA TGGGGACCCA GACTGTTCAG ACCATCAGTG TGGGCTTGCA GACTGAAGCC 
CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC CACAAGTGTC TCACTCCAAA GGCTGGGGGC 
GGTGCTACAC CCGTGTCGTC TCCTTCCCGG AGCCTTAGGA GCAGACAGGT GGCCCCTGCC 
ATCGAGAAGG TGCAGGCCAA GTTTGAACGC ACATGCTGCT CCCCCAAGTA TGGTTCTCCC 
AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC GACCAGCCAA ATAACAGGAC GTCACCAGGG 
ATGGCCCAGA AAGGGTACAG TGAGTCAGCC TGGGCCCGCT CCACCACCAC AAGGGAGAGC 
CCCGTGCACA CCACCATTAA TGATGGCCTC TCCAGCCTCT TCAACATCAT TGACCACAGC 
CCCGTGGTGC AGGACCCCTT CCAGAAGGGG CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA 
GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG GAAACAGGCA CCAATTCCCG AGGAAGGTCG 
CCTAGCCCCA TTGGGGTGGG GTCAGAGATG TGCAGGGAGG AAGGGGGAGA GGGCACGCCA 
GTGAAGCAGG ACTTATCTGC TCCCCCTGGC TACACGCTCA CTGAGAACGT GGCCCGGATC 
CTCAACAAGA AGCTGCTGGA ACATGCCTTA AAGGAGGAGA GGAGGCAGGC TGCCCACGGG 
CCCCCGGGTC TCCACAGTGA CAGCCACTCG CTGGGGGACA CAGCCGAGCC AGGGCCCATG 
GAGGAACTAC CTTGTTCTGC ACTAGCTCCA TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG 
AGACCAGCAA ACCGTCGCCC TCCGTCCCGT TGGGCCCCAC ATTCCCCCAC TGCCTCACAG 
CCTCAGTCAC CCGGAGACCC GACGTCCTTG GAGGAGCATG GTGGCGAGGA GCCGCCCGAG 
GAGCAGCCAC ACCGAGATGC AAGCTTGCAT GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451114.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306882.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X6, mRNA.

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CloneID	OHu104518
Clone ID Related Accession (Same CDS sequence)	XM_024451114.1
Accession Version	XM_024451114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306882.1
CDS	159..5246
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451114.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451114.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451115.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306883.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X7, mRNA.

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CloneID	OHu104519
Clone ID Related Accession (Same CDS sequence)	XM_024451115.1
Accession Version	XM_024451115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGGTG 
ACAAATGGTG TCTTCTTTGG GCTACTGTTA CCCCATCCTT CTGGGGTCCT CAGGAACTAC 
CTTGTTCTGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306883.1
CDS	159..5213
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEVTNGVFFGLLLPHPSGVLRNYLVLH

Target ORF information:

RefSeq Version	XM_024451115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451115.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGGTG 
ACAAATGGTG TCTTCTTTGG GCTACTGTTA CCCCATCCTT CTGGGGTCCT CAGGAACTAC 
CTTGTTCTGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451116.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306884.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

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CloneID	OHu104520
Clone ID Related Accession (Same CDS sequence)	XM_024451116.1
Accession Version	XM_024451116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGAAC 
CAAACTGTCT TGCTAACTGC CCCCTGGGGA CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306884.1
CDS	159..5174
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_024451116.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451116.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGAATTG TTGTGGATAT 
CCCAGAATTA ACATTGAGGA GGAGACTTTA GGCTTCACCA GGCTGCCAGC TGGGTCCACG 
GTAAAAACGT TGAAGAGCCT TGGGTTGCAG AGATTGGAGC TGGAAGAGAA GACTGAGAAC 
AAGTTGGGAG AACTAGGCTC CTCCGCTGAG AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG 
GTGCACCAGA AGCTCCTGGC AGACAGTCAC AGCCTGGTCA TGGACCTGCG CTGGCAGATC 
CATCACAGCG AGAAGAACTG GAACCGGGAG AAGGTGGAAC TTCTCGACCG CCTGGACAGA 
GATCGGCAGG AGTGGGAGCG GCAGAAGAAG GAATTCTTGT GGAGGATAGA GCAGTTGCAG 
AAAGAGAACA GTCCCCGGAG AGGTGGCAGT TTCCTCTGTG ATCAAAAAGA CGGCAACGTT 
CGCCCCTTTC CCCACCAGGG AAGCCTCCGC ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT 
GCAGATGCTG ACTCCATCCC GTTTGAAGAC CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC 
AGGTGCTCGG CCAGTGAGAA TCTCTACCTG GATGCCTTGT CCCTGGATGA CGAGCCAGAA 
GAGCCACCAG CCCACAGGCC CGAGAGGGAG TTCAGGAACC GCCTCCCTGA GGAAGAAGAA 
AATCACAAAG GAAATCTTCA AAGGGCGGTG TCCGTGTCCT CCATGTCTGA GTTCCAGCGT 
CTAATGGACA TCTCCCCCTT CCTGCCTGAG AAGGGCCTGC CGTCCACCAG CAGCAAGGAG 
GATGTCACCC CACCCCTGTC TCCAGACGAC CTCAAGTACA TCGAGGAGTT CAACAAGAGC 
TGGGACTACA CACCCAACAG GGGCCACAAT GGTGGGGGGC CGGACCTTTG GGCCGACAGG 
ACCGAGGTGG GGCGGGCAGG GCACGAGGAC AGCACAGAGC CTTTCCCCGA CTCCTCCTGG 
TACCTAACCA CAAGTGTCAC CATGACCACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCAC TGCGGAGCCA CGTCCTCACC GAGCAGTCGG GGTTGCGCGT GTTACACAGC 
CCGCCTGCCG TGCGCAGGGT CGACAGCATC ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC 
ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC ACCAAGGGAG GCCCTCCAGA ACCCATGCTC 
AGCAGGTGGC CTTGCACCTC CCCCAGGCAC TCCCGGGACT ATGTGGAGGG GGCACGGCGC 
CCCCTTGATA GTCCCCTCTG TACCTCCCTG GGGTTTGCCT CCCCACTGCA CAGCCTGGAG 
ATGTCCAAGA ACTTGAGTGA TGACATGAAG GAGGTGGCCT TCTCTGTCAG GAATGCCATC 
TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGATGGGGA CCCAGACTGT TCAGACCATC AGTGTGGGCT TGCAGACTGA AGCCCTGCGT 
GGCAGCGGTG TCACCAGCAG CCCCCACAAG TGTCTCACTC CAAAGGCTGG GGGCGGTGCT 
ACACCCGTGT CGTCTCCTTC CCGGAGCCTT AGGAGCAGAC AGGTGGCCCC TGCCATCGAG 
AAGGTGCAGG CCAAGTTTGA ACGCACATGC TGCTCCCCCA AGTATGGTTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCCGACCAG CCAAATAACA GGACGTCACC AGGGATGGCC 
CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC CGCTCCACCA CCACAAGGGA GAGCCCCGTG 
CACACCACCA TTAATGATGG CCTCTCCAGC CTCTTCAACA TCATTGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGCTGCGG GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC 
CGACCAGAGC TGGGCCCAGG CCAGGAAACA GGCACCAATT CCCGAGGAAG GTCGCCTAGC 
CCCATTGGGG TGGGGTCAGA GATGTGCAGG GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG 
CAGGACTTAT CTGCTCCCCC TGGCTACACG CTCACTGAGA ACGTGGCCCG GATCCTCAAC 
AAGAAGCTGC TGGAACATGC CTTAAAGGAG GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG 
GGTCTCCACA GTGACAGCCA CTCGCTGGGG GACACAGCCG AGCCAGGGCC CATGGAGAAC 
CAAACTGTCT TGCTAACTGC CCCCTGGGGA CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451117.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306885.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.

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CloneID	OHu104521
Clone ID Related Accession (Same CDS sequence)	XM_024451117.1
Accession Version	XM_024451117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306885.1
CDS	159..5171
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451117.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451117.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451118.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306886.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu104522
Clone ID Related Accession (Same CDS sequence)	XM_024451118.1
Accession Version	XM_024451118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGCTGGAAGA GAAGACTGAG 
AACAAGTTGG GAGAACTAGG CTCCTCCGCT GAGAGCAAGG GGGCCTTGAA GAAGGAGAGA 
GAGGTGCACC AGAAGCTCCT GGCAGACAGT CACAGCCTGG TCATGGACCT GCGCTGGCAG 
ATCCATCACA GCGAGAAGAA CTGGAACCGG GAGAAGGTGG AACTTCTCGA CCGCCTGGAC 
AGAGATCGGC AGGAGTGGGA GCGGCAGAAG AAGGAATTCT TGTGGAGGAT AGAGCAGTTG 
CAGAAAGAGA ACAGTCCCCG GAGAGGTGGC AGTTTCCTCT GTGATCAAAA AGACGGCAAC 
GTTCGCCCCT TTCCCCACCA GGGAAGCCTC CGCATGCCCC GTCCAGTGGC CATGTGGCCT 
TGTGCAGATG CTGACTCCAT CCCGTTTGAA GACCGGCCGC TGTCCAAGCT GAAGGAGTCG 
GACAGGTGCT CGGCCAGTGA GAATCTCTAC CTGGATGCCT TGTCCCTGGA TGACGAGCCA 
GAAGAGCCAC CAGCCCACAG GCCCGAGAGG GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA 
GAAAATCACA AAGGAAATCT TCAAAGGGCG GTGTCCGTGT CCTCCATGTC TGAGTTCCAG 
CGTCTAATGG ACATCTCCCC CTTCCTGCCT GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG 
GAGGATGTCA CCCCACCCCT GTCTCCAGAC GACCTCAAGT ACATCGAGGA GTTCAACAAG 
AGCTGGGACT ACACACCCAA CAGGGGCCAC AATGGTGGGG GGCCGGACCT TTGGGCCGAC 
AGGACCGAGG TGGGGCGGGC AGGGCACGAG GACAGCACAG AGCCTTTCCC CGACTCCTCC 
TGGTACCTAA CCACAAGTGT CACCATGACC ACGGACACCA TGACCAGCCC AGAGCACTGC 
CAGAAGCAGC CACTGCGGAG CCACGTCCTC ACCGAGCAGT CGGGGTTGCG CGTGTTACAC 
AGCCCGCCTG CCGTGCGCAG GGTCGACAGC ATCACGGCGG CAGGTGGTGA GGGTCCCTTT 
CCCACAAGCA GAGCCAGAGG GAGCCCGGGA GACACCAAGG GAGGCCCTCC AGAACCCATG 
CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG CACTCCCGGG ACTATGTGGA GGGGGCACGG 
CGCCCCCTTG ATAGTCCCCT CTGTACCTCC CTGGGGTTTG CCTCCCCACT GCACAGCCTG 
GAGATGTCCA AGAACTTGAG TGATGACATG AAGGAGGTGG CCTTCTCTGT CAGGAATGCC 
ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC AAGGACATGG CCTGCCAGAC CAATGGGTCC 
CGGACGATGG GGACCCAGAC TGTTCAGACC ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG 
CGTGGCAGCG GTGTCACCAG CAGCCCCCAC AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT 
GCTACACCCG TGTCGTCTCC TTCCCGGAGC CTTAGGAGCA GACAGGTGGC CCCTGCCATC 
GAGAAGGTGC AGGCCAAGTT TGAACGCACA TGCTGCTCCC CCAAGTATGG TTCTCCCAAG 
CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC CAGCCAAATA ACAGGACGTC ACCAGGGATG 
GCCCAGAAAG GGTACAGTGA GTCAGCCTGG GCCCGCTCCA CCACCACAAG GGAGAGCCCC 
GTGCACACCA CCATTAATGA TGGCCTCTCC AGCCTCTTCA ACATCATTGA CCACAGCCCC 
GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG 
CCCCGACCAG AGCTGGGCCC AGGCCAGGAA ACAGGCACCA ATTCCCGAGG AAGGTCGCCT 
AGCCCCATTG GGGTGGGGTC AGAGATGTGC AGGGAGGAAG GGGGAGAGGG CACGCCAGTG 
AAGCAGGACT TATCTGCTCC CCCTGGCTAC ACGCTCACTG AGAACGTGGC CCGGATCCTC 
AACAAGAAGC TGCTGGAACA TGCCTTAAAG GAGGAGAGGA GGCAGGCTGC CCACGGGCCC 
CCGGGTCTCC ACAGTGACAG CCACTCGCTG GGGGACACAG CCGAGCCAGG GCCCATGGAG 
GAACTACCTT GTTCTGCACT AGCTCCATCC CTAGAGCCCT GCTTCTCCAG GCCCGAGAGA 
CCAGCAAACC GTCGCCCTCC GTCCCGTTGG GCCCCACATT CCCCCACTGC CTCACAGCCT 
CAGTCACCCG GAGACCCGAC GTCCTTGGAG GAGCATGGTG GCGAGGAGCC GCCCGAGGAG 
CAGCCACACC GAGATGCAAG CTTGCATGGA TTATCACAGT ATAATTCACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306886.1
CDS	159..5132
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451118.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451118.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGCTGGAAGA GAAGACTGAG 
AACAAGTTGG GAGAACTAGG CTCCTCCGCT GAGAGCAAGG GGGCCTTGAA GAAGGAGAGA 
GAGGTGCACC AGAAGCTCCT GGCAGACAGT CACAGCCTGG TCATGGACCT GCGCTGGCAG 
ATCCATCACA GCGAGAAGAA CTGGAACCGG GAGAAGGTGG AACTTCTCGA CCGCCTGGAC 
AGAGATCGGC AGGAGTGGGA GCGGCAGAAG AAGGAATTCT TGTGGAGGAT AGAGCAGTTG 
CAGAAAGAGA ACAGTCCCCG GAGAGGTGGC AGTTTCCTCT GTGATCAAAA AGACGGCAAC 
GTTCGCCCCT TTCCCCACCA GGGAAGCCTC CGCATGCCCC GTCCAGTGGC CATGTGGCCT 
TGTGCAGATG CTGACTCCAT CCCGTTTGAA GACCGGCCGC TGTCCAAGCT GAAGGAGTCG 
GACAGGTGCT CGGCCAGTGA GAATCTCTAC CTGGATGCCT TGTCCCTGGA TGACGAGCCA 
GAAGAGCCAC CAGCCCACAG GCCCGAGAGG GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA 
GAAAATCACA AAGGAAATCT TCAAAGGGCG GTGTCCGTGT CCTCCATGTC TGAGTTCCAG 
CGTCTAATGG ACATCTCCCC CTTCCTGCCT GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG 
GAGGATGTCA CCCCACCCCT GTCTCCAGAC GACCTCAAGT ACATCGAGGA GTTCAACAAG 
AGCTGGGACT ACACACCCAA CAGGGGCCAC AATGGTGGGG GGCCGGACCT TTGGGCCGAC 
AGGACCGAGG TGGGGCGGGC AGGGCACGAG GACAGCACAG AGCCTTTCCC CGACTCCTCC 
TGGTACCTAA CCACAAGTGT CACCATGACC ACGGACACCA TGACCAGCCC AGAGCACTGC 
CAGAAGCAGC CACTGCGGAG CCACGTCCTC ACCGAGCAGT CGGGGTTGCG CGTGTTACAC 
AGCCCGCCTG CCGTGCGCAG GGTCGACAGC ATCACGGCGG CAGGTGGTGA GGGTCCCTTT 
CCCACAAGCA GAGCCAGAGG GAGCCCGGGA GACACCAAGG GAGGCCCTCC AGAACCCATG 
CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG CACTCCCGGG ACTATGTGGA GGGGGCACGG 
CGCCCCCTTG ATAGTCCCCT CTGTACCTCC CTGGGGTTTG CCTCCCCACT GCACAGCCTG 
GAGATGTCCA AGAACTTGAG TGATGACATG AAGGAGGTGG CCTTCTCTGT CAGGAATGCC 
ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC AAGGACATGG CCTGCCAGAC CAATGGGTCC 
CGGACGATGG GGACCCAGAC TGTTCAGACC ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG 
CGTGGCAGCG GTGTCACCAG CAGCCCCCAC AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT 
GCTACACCCG TGTCGTCTCC TTCCCGGAGC CTTAGGAGCA GACAGGTGGC CCCTGCCATC 
GAGAAGGTGC AGGCCAAGTT TGAACGCACA TGCTGCTCCC CCAAGTATGG TTCTCCCAAG 
CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC CAGCCAAATA ACAGGACGTC ACCAGGGATG 
GCCCAGAAAG GGTACAGTGA GTCAGCCTGG GCCCGCTCCA CCACCACAAG GGAGAGCCCC 
GTGCACACCA CCATTAATGA TGGCCTCTCC AGCCTCTTCA ACATCATTGA CCACAGCCCC 
GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG 
CCCCGACCAG AGCTGGGCCC AGGCCAGGAA ACAGGCACCA ATTCCCGAGG AAGGTCGCCT 
AGCCCCATTG GGGTGGGGTC AGAGATGTGC AGGGAGGAAG GGGGAGAGGG CACGCCAGTG 
AAGCAGGACT TATCTGCTCC CCCTGGCTAC ACGCTCACTG AGAACGTGGC CCGGATCCTC 
AACAAGAAGC TGCTGGAACA TGCCTTAAAG GAGGAGAGGA GGCAGGCTGC CCACGGGCCC 
CCGGGTCTCC ACAGTGACAG CCACTCGCTG GGGGACACAG CCGAGCCAGG GCCCATGGAG 
GAACTACCTT GTTCTGCACT AGCTCCATCC CTAGAGCCCT GCTTCTCCAG GCCCGAGAGA 
CCAGCAAACC GTCGCCCTCC GTCCCGTTGG GCCCCACATT CCCCCACTGC CTCACAGCCT 
CAGTCACCCG GAGACCCGAC GTCCTTGGAG GAGCATGGTG GCGAGGAGCC GCCCGAGGAG 
CAGCCACACC GAGATGCAAG CTTGCATGGA TTATCACAGT ATAATTCACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451119.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306887.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu104523
Clone ID Related Accession (Same CDS sequence)	XM_024451119.1
Accession Version	XM_024451119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
TTGCAGAAAG AGAACAGTCC CCGGAGAGGT GGCAGTTTCC TCTGTGATCA AAAAGACGGC 
AACGTTCGCC CCTTTCCCCA CCAGGGAAGC CTCCGCATGC CCCGTCCAGT GGCCATGTGG 
CCTTGTGCAG ATGCTGACTC CATCCCGTTT GAAGACCGGC CGCTGTCCAA GCTGAAGGAG 
TCGGACAGGT GCTCGGCCAG TGAGAATCTC TACCTGGATG CCTTGTCCCT GGATGACGAG 
CCAGAAGAGC CACCAGCCCA CAGGCCCGAG AGGGAGTTCA GGAACCGCCT CCCTGAGGAA 
GAAGAAAATC ACAAAGGAAA TCTTCAAAGG GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTAA TGGACATCTC CCCCTTCCTG CCTGAGAAGG GCCTGCCGTC CACCAGCAGC 
AAGGAGGATG TCACCCCACC CCTGTCTCCA GACGACCTCA AGTACATCGA GGAGTTCAAC 
AAGAGCTGGG ACTACACACC CAACAGGGGC CACAATGGTG GGGGGCCGGA CCTTTGGGCC 
GACAGGACCG AGGTGGGGCG GGCAGGGCAC GAGGACAGCA CAGAGCCTTT CCCCGACTCC 
TCCTGGTACC TAACCACAAG TGTCACCATG ACCACGGACA CCATGACCAG CCCAGAGCAC 
TGCCAGAAGC AGCCACTGCG GAGCCACGTC CTCACCGAGC AGTCGGGGTT GCGCGTGTTA 
CACAGCCCGC CTGCCGTGCG CAGGGTCGAC AGCATCACGG CGGCAGGTGG TGAGGGTCCC 
TTTCCCACAA GCAGAGCCAG AGGGAGCCCG GGAGACACCA AGGGAGGCCC TCCAGAACCC 
ATGCTCAGCA GGTGGCCTTG CACCTCCCCC AGGCACTCCC GGGACTATGT GGAGGGGGCA 
CGGCGCCCCC TTGATAGTCC CCTCTGTACC TCCCTGGGGT TTGCCTCCCC ACTGCACAGC 
CTGGAGATGT CCAAGAACTT GAGTGATGAC ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT 
GCCATCTGCT CCGGCCCTGG CGAGCTGCAA GTCAAGGACA TGGCCTGCCA GACCAATGGG 
TCCCGGACGA TGGGGACCCA GACTGTTCAG ACCATCAGTG TGGGCTTGCA GACTGAAGCC 
CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC CACAAGTGTC TCACTCCAAA GGCTGGGGGC 
GGTGCTACAC CCGTGTCGTC TCCTTCCCGG AGCCTTAGGA GCAGACAGGT GGCCCCTGCC 
ATCGAGAAGG TGCAGGCCAA GTTTGAACGC ACATGCTGCT CCCCCAAGTA TGGTTCTCCC 
AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC GACCAGCCAA ATAACAGGAC GTCACCAGGG 
ATGGCCCAGA AAGGGTACAG TGAGTCAGCC TGGGCCCGCT CCACCACCAC AAGGGAGAGC 
CCCGTGCACA CCACCATTAA TGATGGCCTC TCCAGCCTCT TCAACATCAT TGACCACAGC 
CCCGTGGTGC AGGACCCCTT CCAGAAGGGG CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA 
GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG GAAACAGGCA CCAATTCCCG AGGAAGGTCG 
CCTAGCCCCA TTGGGGTGGG GTCAGAGATG TGCAGGGAGG AAGGGGGAGA GGGCACGCCA 
GTGAAGCAGG ACTTATCTGC TCCCCCTGGC TACACGCTCA CTGAGAACGT GGCCCGGATC 
CTCAACAAGA AGCTGCTGGA ACATGCCTTA AAGGAGGAGA GGAGGCAGGC TGCCCACGGG 
CCCCCGGGTC TCCACAGTGA CAGCCACTCG CTGGGGGACA CAGCCGAGCC AGGGCCCATG 
GAGAACCAAA CTGTCTTGCT AACTGCCCCC TGGGGACTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306887.1
CDS	159..5060
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_024451119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451119.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
TTGCAGAAAG AGAACAGTCC CCGGAGAGGT GGCAGTTTCC TCTGTGATCA AAAAGACGGC 
AACGTTCGCC CCTTTCCCCA CCAGGGAAGC CTCCGCATGC CCCGTCCAGT GGCCATGTGG 
CCTTGTGCAG ATGCTGACTC CATCCCGTTT GAAGACCGGC CGCTGTCCAA GCTGAAGGAG 
TCGGACAGGT GCTCGGCCAG TGAGAATCTC TACCTGGATG CCTTGTCCCT GGATGACGAG 
CCAGAAGAGC CACCAGCCCA CAGGCCCGAG AGGGAGTTCA GGAACCGCCT CCCTGAGGAA 
GAAGAAAATC ACAAAGGAAA TCTTCAAAGG GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTAA TGGACATCTC CCCCTTCCTG CCTGAGAAGG GCCTGCCGTC CACCAGCAGC 
AAGGAGGATG TCACCCCACC CCTGTCTCCA GACGACCTCA AGTACATCGA GGAGTTCAAC 
AAGAGCTGGG ACTACACACC CAACAGGGGC CACAATGGTG GGGGGCCGGA CCTTTGGGCC 
GACAGGACCG AGGTGGGGCG GGCAGGGCAC GAGGACAGCA CAGAGCCTTT CCCCGACTCC 
TCCTGGTACC TAACCACAAG TGTCACCATG ACCACGGACA CCATGACCAG CCCAGAGCAC 
TGCCAGAAGC AGCCACTGCG GAGCCACGTC CTCACCGAGC AGTCGGGGTT GCGCGTGTTA 
CACAGCCCGC CTGCCGTGCG CAGGGTCGAC AGCATCACGG CGGCAGGTGG TGAGGGTCCC 
TTTCCCACAA GCAGAGCCAG AGGGAGCCCG GGAGACACCA AGGGAGGCCC TCCAGAACCC 
ATGCTCAGCA GGTGGCCTTG CACCTCCCCC AGGCACTCCC GGGACTATGT GGAGGGGGCA 
CGGCGCCCCC TTGATAGTCC CCTCTGTACC TCCCTGGGGT TTGCCTCCCC ACTGCACAGC 
CTGGAGATGT CCAAGAACTT GAGTGATGAC ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT 
GCCATCTGCT CCGGCCCTGG CGAGCTGCAA GTCAAGGACA TGGCCTGCCA GACCAATGGG 
TCCCGGACGA TGGGGACCCA GACTGTTCAG ACCATCAGTG TGGGCTTGCA GACTGAAGCC 
CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC CACAAGTGTC TCACTCCAAA GGCTGGGGGC 
GGTGCTACAC CCGTGTCGTC TCCTTCCCGG AGCCTTAGGA GCAGACAGGT GGCCCCTGCC 
ATCGAGAAGG TGCAGGCCAA GTTTGAACGC ACATGCTGCT CCCCCAAGTA TGGTTCTCCC 
AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC GACCAGCCAA ATAACAGGAC GTCACCAGGG 
ATGGCCCAGA AAGGGTACAG TGAGTCAGCC TGGGCCCGCT CCACCACCAC AAGGGAGAGC 
CCCGTGCACA CCACCATTAA TGATGGCCTC TCCAGCCTCT TCAACATCAT TGACCACAGC 
CCCGTGGTGC AGGACCCCTT CCAGAAGGGG CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA 
GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG GAAACAGGCA CCAATTCCCG AGGAAGGTCG 
CCTAGCCCCA TTGGGGTGGG GTCAGAGATG TGCAGGGAGG AAGGGGGAGA GGGCACGCCA 
GTGAAGCAGG ACTTATCTGC TCCCCCTGGC TACACGCTCA CTGAGAACGT GGCCCGGATC 
CTCAACAAGA AGCTGCTGGA ACATGCCTTA AAGGAGGAGA GGAGGCAGGC TGCCCACGGG 
CCCCCGGGTC TCCACAGTGA CAGCCACTCG CTGGGGGACA CAGCCGAGCC AGGGCCCATG 
GAGAACCAAA CTGTCTTGCT AACTGCCCCC TGGGGACTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005258098.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258155.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.

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CloneID	OHu104524
Clone ID Related Accession (Same CDS sequence)	XM_005258098.4
Accession Version	XM_005258098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258098.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGGAACTACC TTGTTCTGCA 
CTAGCTCCAT CCCTAGAGCC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCCT 
CCGTCCCGTT GGGCCCCACA TTCCCCCACT GCCTCACAGC CTCAGTCACC CGGAGACCCG 
ACGTCCTTGG AGGAGCATGG TGGCGAGGAG CCGCCCGAGG AGCAGCCACA CCGAGATGCA 
AGCTTGCATG GATTATCACA GTATAATTCA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258155.2
CDS	624..5519
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_005258098.4
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258098.4

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGGAACTACC TTGTTCTGCA 
CTAGCTCCAT CCCTAGAGCC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCCT 
CCGTCCCGTT GGGCCCCACA TTCCCCCACT GCCTCACAGC CTCAGTCACC CGGAGACCCG 
ACGTCCTTGG AGGAGCATGG TGGCGAGGAG CCGCCCGAGG AGCAGCCACA CCGAGATGCA 
AGCTTGCATG GATTATCACA GTATAATTCA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_011525641.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523943.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.

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CloneID	OHu104525
Clone ID Related Accession (Same CDS sequence)	XM_011525641.3
Accession Version	XM_011525641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG CTCAGGGGCC CCCCCGTTTT ACCTGAGCAG 
AGTGTATCCA TAGAGGAGCT ACAGGGTCAG CTCGTGCAGG CGGCCAGACT GCATCAAGAG 
GAGACAGAGA CATTTACAAA CAAGATCCAT AAGATGGAGG AGGAGCACCT CTATGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAAA ACACCCTCCA TGAGCGAACC 
TGGAGTGATG AGAAGAATCT GATGCAGCAG GAGCTCCGGT CCTTGAAGCA GAACATTTTC 
CTCTTCTACG TCAAACTCAG GTGGCTGCTG AAACACTGGC GGCAAGGGAA GCAGATGGAG 
GAGGAAGGAG AGGAGTTCAC TGAGGGTGAA CATCCAGAGA CCCTCTCCAG GCTCGGGGAG 
CTTGGAGTCC AGGGGGGTCA CCAGGCGGAT GGCCCAGACC ACGACAGTGA CCGAGGCTGT 
GGCTTTCCAG TGGGGGAGCA CTCCCCACAC TCCCGGGTGC AGATTGGAGA TCACAGCTTG 
CGGCTGCAGA CCGCGGACAG GGGACAGCCC CACAAACAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGCGCCT TCAAGGCCTT GCTGGAGGAC TTCCGTGCGG AGCTGCGGGA GGATGAGCGT 
GCCCGACTAC GGCTGCAGCA GCAATATGCC AGCGACAAGG CGGCCTGGGA CGTGGAGTGG 
GCCGTGCTCA AGTGCCGTCT GGAACAGCTG GAAGAGAAGA CTGAGAACAA GTTGGGAGAA 
CTAGGCTCCT CCGCTGAGAG CAAGGGGGCC TTGAAGAAGG AGAGAGAGGT GCACCAGAAG 
CTCCTGGCAG ACAGTCACAG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGCGAG 
AAGAACTGGA ACCGGGAGAA GGTGGAACTT CTCGACCGCC TGGACAGAGA TCGGCAGGAG 
TGGGAGCGGC AGAAGAAGGA ATTCTTGTGG AGGATAGAGC AGTTGCAGAA AGAGAACAGT 
CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT CAAAAAGACG GCAACGTTCG CCCCTTTCCC 
CACCAGGGAA GCCTCCGCAT GCCCCGTCCA GTGGCCATGT GGCCTTGTGC AGATGCTGAC 
TCCATCCCGT TTGAAGACCG GCCGCTGTCC AAGCTGAAGG AGTCGGACAG GTGCTCGGCC 
AGTGAGAATC TCTACCTGGA TGCCTTGTCC CTGGATGACG AGCCAGAAGA GCCACCAGCC 
CACAGGCCCG AGAGGGAGTT CAGGAACCGC CTCCCTGAGG AAGAAGAAAA TCACAAAGGA 
AATCTTCAAA GGGCGGTGTC CGTGTCCTCC ATGTCTGAGT TCCAGCGTCT AATGGACATC 
TCCCCCTTCC TGCCTGAGAA GGGCCTGCCG TCCACCAGCA GCAAGGAGGA TGTCACCCCA 
CCCCTGTCTC CAGACGACCT CAAGTACATC GAGGAGTTCA ACAAGAGCTG GGACTACACA 
CCCAACAGGG GCCACAATGG TGGGGGGCCG GACCTTTGGG CCGACAGGAC CGAGGTGGGG 
CGGGCAGGGC ACGAGGACAG CACAGAGCCT TTCCCCGACT CCTCCTGGTA CCTAACCACA 
AGTGTCACCA TGACCACGGA CACCATGACC AGCCCAGAGC ACTGCCAGAA GCAGCCACTG 
CGGAGCCACG TCCTCACCGA GCAGTCGGGG TTGCGCGTGT TACACAGCCC GCCTGCCGTG 
CGCAGGGTCG ACAGCATCAC GGCGGCAGGT GGTGAGGGTC CCTTTCCCAC AAGCAGAGCC 
AGAGGGAGCC CGGGAGACAC CAAGGGAGGC CCTCCAGAAC CCATGCTCAG CAGGTGGCCT 
TGCACCTCCC CCAGGCACTC CCGGGACTAT GTGGAGGGGG CACGGCGCCC CCTTGATAGT 
CCCCTCTGTA CCTCCCTGGG GTTTGCCTCC CCACTGCACA GCCTGGAGAT GTCCAAGAAC 
TTGAGTGATG ACATGAAGGA GGTGGCCTTC TCTGTCAGGA ATGCCATCTG CTCCGGCCCT 
GGCGAGCTGC AAGTCAAGGA CATGGCCTGC CAGACCAATG GGTCCCGGAC GATGGGGACC 
CAGACTGTTC AGACCATCAG TGTGGGCTTG CAGACTGAAG CCCTGCGTGG CAGCGGTGTC 
ACCAGCAGCC CCCACAAGTG TCTCACTCCA AAGGCTGGGG GCGGTGCTAC ACCCGTGTCG 
TCTCCTTCCC GGAGCCTTAG GAGCAGACAG GTGGCCCCTG CCATCGAGAA GGTGCAGGCC 
AAGTTTGAAC GCACATGCTG CTCCCCCAAG TATGGTTCTC CCAAGCTGCA GAGGAAGCCC 
CTCCCCAAAG CCGACCAGCC AAATAACAGG ACGTCACCAG GGATGGCCCA GAAAGGGTAC 
AGTGAGTCAG CCTGGGCCCG CTCCACCACC ACAAGGGAGA GCCCCGTGCA CACCACCATT 
AATGATGGCC TCTCCAGCCT CTTCAACATC ATTGACCACA GCCCCGTGGT GCAGGACCCC 
TTCCAGAAGG GGCTGCGGGC CGGCAGTCGG TCTCGCTCAG CAGAGCCCCG ACCAGAGCTG 
GGCCCAGGCC AGGAAACAGG CACCAATTCC CGAGGAAGGT CGCCTAGCCC CATTGGGGTG 
GGGTCAGAGA TGTGCAGGGA GGAAGGGGGA GAGGGCACGC CAGTGAAGCA GGACTTATCT 
GCTCCCCCTG GCTACACGCT CACTGAGAAC GTGGCCCGGA TCCTCAACAA GAAGCTGCTG 
GAACATGCCT TAAAGGAGGA GAGGAGGCAG GCTGCCCACG GGCCCCCGGG TCTCCACAGT 
GACAGCCACT CGCTGGGGGA CACAGCCGAG CCAGGGCCCA TGGAGAACCA AACTGTCTTG 
CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523943.2
CDS	159..4982
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_011525641.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525641.3

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG CTCAGGGGCC CCCCCGTTTT ACCTGAGCAG 
AGTGTATCCA TAGAGGAGCT ACAGGGTCAG CTCGTGCAGG CGGCCAGACT GCATCAAGAG 
GAGACAGAGA CATTTACAAA CAAGATCCAT AAGATGGAGG AGGAGCACCT CTATGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAAA ACACCCTCCA TGAGCGAACC 
TGGAGTGATG AGAAGAATCT GATGCAGCAG GAGCTCCGGT CCTTGAAGCA GAACATTTTC 
CTCTTCTACG TCAAACTCAG GTGGCTGCTG AAACACTGGC GGCAAGGGAA GCAGATGGAG 
GAGGAAGGAG AGGAGTTCAC TGAGGGTGAA CATCCAGAGA CCCTCTCCAG GCTCGGGGAG 
CTTGGAGTCC AGGGGGGTCA CCAGGCGGAT GGCCCAGACC ACGACAGTGA CCGAGGCTGT 
GGCTTTCCAG TGGGGGAGCA CTCCCCACAC TCCCGGGTGC AGATTGGAGA TCACAGCTTG 
CGGCTGCAGA CCGCGGACAG GGGACAGCCC CACAAACAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGCGCCT TCAAGGCCTT GCTGGAGGAC TTCCGTGCGG AGCTGCGGGA GGATGAGCGT 
GCCCGACTAC GGCTGCAGCA GCAATATGCC AGCGACAAGG CGGCCTGGGA CGTGGAGTGG 
GCCGTGCTCA AGTGCCGTCT GGAACAGCTG GAAGAGAAGA CTGAGAACAA GTTGGGAGAA 
CTAGGCTCCT CCGCTGAGAG CAAGGGGGCC TTGAAGAAGG AGAGAGAGGT GCACCAGAAG 
CTCCTGGCAG ACAGTCACAG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGCGAG 
AAGAACTGGA ACCGGGAGAA GGTGGAACTT CTCGACCGCC TGGACAGAGA TCGGCAGGAG 
TGGGAGCGGC AGAAGAAGGA ATTCTTGTGG AGGATAGAGC AGTTGCAGAA AGAGAACAGT 
CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT CAAAAAGACG GCAACGTTCG CCCCTTTCCC 
CACCAGGGAA GCCTCCGCAT GCCCCGTCCA GTGGCCATGT GGCCTTGTGC AGATGCTGAC 
TCCATCCCGT TTGAAGACCG GCCGCTGTCC AAGCTGAAGG AGTCGGACAG GTGCTCGGCC 
AGTGAGAATC TCTACCTGGA TGCCTTGTCC CTGGATGACG AGCCAGAAGA GCCACCAGCC 
CACAGGCCCG AGAGGGAGTT CAGGAACCGC CTCCCTGAGG AAGAAGAAAA TCACAAAGGA 
AATCTTCAAA GGGCGGTGTC CGTGTCCTCC ATGTCTGAGT TCCAGCGTCT AATGGACATC 
TCCCCCTTCC TGCCTGAGAA GGGCCTGCCG TCCACCAGCA GCAAGGAGGA TGTCACCCCA 
CCCCTGTCTC CAGACGACCT CAAGTACATC GAGGAGTTCA ACAAGAGCTG GGACTACACA 
CCCAACAGGG GCCACAATGG TGGGGGGCCG GACCTTTGGG CCGACAGGAC CGAGGTGGGG 
CGGGCAGGGC ACGAGGACAG CACAGAGCCT TTCCCCGACT CCTCCTGGTA CCTAACCACA 
AGTGTCACCA TGACCACGGA CACCATGACC AGCCCAGAGC ACTGCCAGAA GCAGCCACTG 
CGGAGCCACG TCCTCACCGA GCAGTCGGGG TTGCGCGTGT TACACAGCCC GCCTGCCGTG 
CGCAGGGTCG ACAGCATCAC GGCGGCAGGT GGTGAGGGTC CCTTTCCCAC AAGCAGAGCC 
AGAGGGAGCC CGGGAGACAC CAAGGGAGGC CCTCCAGAAC CCATGCTCAG CAGGTGGCCT 
TGCACCTCCC CCAGGCACTC CCGGGACTAT GTGGAGGGGG CACGGCGCCC CCTTGATAGT 
CCCCTCTGTA CCTCCCTGGG GTTTGCCTCC CCACTGCACA GCCTGGAGAT GTCCAAGAAC 
TTGAGTGATG ACATGAAGGA GGTGGCCTTC TCTGTCAGGA ATGCCATCTG CTCCGGCCCT 
GGCGAGCTGC AAGTCAAGGA CATGGCCTGC CAGACCAATG GGTCCCGGAC GATGGGGACC 
CAGACTGTTC AGACCATCAG TGTGGGCTTG CAGACTGAAG CCCTGCGTGG CAGCGGTGTC 
ACCAGCAGCC CCCACAAGTG TCTCACTCCA AAGGCTGGGG GCGGTGCTAC ACCCGTGTCG 
TCTCCTTCCC GGAGCCTTAG GAGCAGACAG GTGGCCCCTG CCATCGAGAA GGTGCAGGCC 
AAGTTTGAAC GCACATGCTG CTCCCCCAAG TATGGTTCTC CCAAGCTGCA GAGGAAGCCC 
CTCCCCAAAG CCGACCAGCC AAATAACAGG ACGTCACCAG GGATGGCCCA GAAAGGGTAC 
AGTGAGTCAG CCTGGGCCCG CTCCACCACC ACAAGGGAGA GCCCCGTGCA CACCACCATT 
AATGATGGCC TCTCCAGCCT CTTCAACATC ATTGACCACA GCCCCGTGGT GCAGGACCCC 
TTCCAGAAGG GGCTGCGGGC CGGCAGTCGG TCTCGCTCAG CAGAGCCCCG ACCAGAGCTG 
GGCCCAGGCC AGGAAACAGG CACCAATTCC CGAGGAAGGT CGCCTAGCCC CATTGGGGTG 
GGGTCAGAGA TGTGCAGGGA GGAAGGGGGA GAGGGCACGC CAGTGAAGCA GGACTTATCT 
GCTCCCCCTG GCTACACGCT CACTGAGAAC GTGGCCCGGA TCCTCAACAA GAAGCTGCTG 
GAACATGCCT TAAAGGAGGA GAGGAGGCAG GCTGCCCACG GGCCCCCGGG TCTCCACAGT 
GACAGCCACT CGCTGGGGGA CACAGCCGAG CCAGGGCCCA TGGAGAACCA AACTGTCTTG 
CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451120.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306888.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.

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CloneID	OHu104526
Clone ID Related Accession (Same CDS sequence)	XM_024451120.1
Accession Version	XM_024451120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGA ACCAAACTGT CTTGCTAACT 
GCCCCCTGGG GACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306888.1
CDS	159..4976
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_024451120.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451120.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC 
CCCGTTTTAC CTGAGCAGAG TGTATCCATA GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG 
GCCAGACTGC ATCAAGAGGA GACAGAGACA TTTACAAACA AGATCCATAA GATGGAGGAG 
GAGCACCTCT ATGCCTTGAG GTGGAAAGAA CTGGAAATGC ACAGCCTGGC TTTGCAAAAC 
ACCCTCCATG AGCGAACCTG GAGTGATGAG AAGAATCTGA TGCAGCAGGA GCTCCGGTCC 
TTGAAGCAGA ACATTTTCCT CTTCTACGTC AAACTCAGGT GGCTGCTGAA ACACTGGCGG 
CAAGGGAAGC AGATGGAGGA GGAAGGAGAG GAGTTCACTG AGGGTGAACA TCCAGAGACC 
CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG GGGGGTCACC AGGCGGATGG CCCAGACCAC 
GACAGTGACC GAGGCTGTGG CTTTCCAGTG GGGGAGCACT CCCCACACTC CCGGGTGCAG 
ATTGGAGATC ACAGCTTGCG GCTGCAGACC GCGGACAGGG GACAGCCCCA CAAACAGGTG 
GTGGAAAACC AGCAGCTGTT CAGCGCCTTC AAGGCCTTGC TGGAGGACTT CCGTGCGGAG 
CTGCGGGAGG ATGAGCGTGC CCGACTACGG CTGCAGCAGC AATATGCCAG CGACAAGGCG 
GCCTGGGACG TGGAGTGGGC CGTGCTCAAG TGCCGTCTGG AACAGCTGGA AGAGAAGACT 
GAGAACAAGT TGGGAGAACT AGGCTCCTCC GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG 
AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC AGTCACAGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGCGAGAA GAACTGGAAC CGGGAGAAGG TGGAACTTCT CGACCGCCTG 
GACAGAGATC GGCAGGAGTG GGAGCGGCAG AAGAAGGAAT TCTTGTGGAG GATAGAGCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGA ACCAAACTGT CTTGCTAACT 
GCCCCCTGGG GACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451121.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306889.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.

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CloneID	OHu104527
Clone ID Related Accession (Same CDS sequence)	XM_024451121.1
Accession Version	XM_024451121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGCTGGAAGA GAAGACTGAG 
AACAAGTTGG GAGAACTAGG CTCCTCCGCT GAGAGCAAGG GGGCCTTGAA GAAGGAGAGA 
GAGGTGCACC AGAAGCTCCT GGCAGACAGT CACAGCCTGG TCATGGACCT GCGCTGGCAG 
ATCCATCACA GCGAGAAGAA CTGGAACCGG GAGAAGGTGG AACTTCTCGA CCGCCTGGAC 
AGAGATCGGC AGGAGTGGGA GCGGCAGAAG AAGGAATTCT TGTGGAGGAT AGAGCAGTTG 
CAGAAAGAGA ACAGTCCCCG GAGAGGTGGC AGTTTCCTCT GTGATCAAAA AGACGGCAAC 
GTTCGCCCCT TTCCCCACCA GGGAAGCCTC CGCATGCCCC GTCCAGTGGC CATGTGGCCT 
TGTGCAGATG CTGACTCCAT CCCGTTTGAA GACCGGCCGC TGTCCAAGCT GAAGGAGTCG 
GACAGGTGCT CGGCCAGTGA GAATCTCTAC CTGGATGCCT TGTCCCTGGA TGACGAGCCA 
GAAGAGCCAC CAGCCCACAG GCCCGAGAGG GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA 
GAAAATCACA AAGGAAATCT TCAAAGGGCG GTGTCCGTGT CCTCCATGTC TGAGTTCCAG 
CGTCTAATGG ACATCTCCCC CTTCCTGCCT GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG 
GAGGATGTCA CCCCACCCCT GTCTCCAGAC GACCTCAAGT ACATCGAGGA GTTCAACAAG 
AGCTGGGACT ACACACCCAA CAGGGGCCAC AATGGTGGGG GGCCGGACCT TTGGGCCGAC 
AGGACCGAGG TGGGGCGGGC AGGGCACGAG GACAGCACAG AGCCTTTCCC CGACTCCTCC 
TGGTACCTAA CCACAAGTGT CACCATGACC ACGGACACCA TGACCAGCCC AGAGCACTGC 
CAGAAGCAGC CACTGCGGAG CCACGTCCTC ACCGAGCAGT CGGGGTTGCG CGTGTTACAC 
AGCCCGCCTG CCGTGCGCAG GGTCGACAGC ATCACGGCGG CAGGTGGTGA GGGTCCCTTT 
CCCACAAGCA GAGCCAGAGG GAGCCCGGGA GACACCAAGG GAGGCCCTCC AGAACCCATG 
CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG CACTCCCGGG ACTATGTGGA GGGGGCACGG 
CGCCCCCTTG ATAGTCCCCT CTGTACCTCC CTGGGGTTTG CCTCCCCACT GCACAGCCTG 
GAGATGTCCA AGAACTTGAG TGATGACATG AAGGAGGTGG CCTTCTCTGT CAGGAATGCC 
ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC AAGGACATGG CCTGCCAGAC CAATGGGTCC 
CGGACGATGG GGACCCAGAC TGTTCAGACC ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG 
CGTGGCAGCG GTGTCACCAG CAGCCCCCAC AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT 
GCTACACCCG TGTCGTCTCC TTCCCGGAGC CTTAGGAGCA GACAGGTGGC CCCTGCCATC 
GAGAAGGTGC AGGCCAAGTT TGAACGCACA TGCTGCTCCC CCAAGTATGG TTCTCCCAAG 
CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC CAGCCAAATA ACAGGACGTC ACCAGGGATG 
GCCCAGAAAG GGTACAGTGA GTCAGCCTGG GCCCGCTCCA CCACCACAAG GGAGAGCCCC 
GTGCACACCA CCATTAATGA TGGCCTCTCC AGCCTCTTCA ACATCATTGA CCACAGCCCC 
GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG 
CCCCGACCAG AGCTGGGCCC AGGCCAGGAA ACAGGCACCA ATTCCCGAGG AAGGTCGCCT 
AGCCCCATTG GGGTGGGGTC AGAGATGTGC AGGGAGGAAG GGGGAGAGGG CACGCCAGTG 
AAGCAGGACT TATCTGCTCC CCCTGGCTAC ACGCTCACTG AGAACGTGGC CCGGATCCTC 
AACAAGAAGC TGCTGGAACA TGCCTTAAAG GAGGAGAGGA GGCAGGCTGC CCACGGGCCC 
CCGGGTCTCC ACAGTGACAG CCACTCGCTG GGGGACACAG CCGAGCCAGG GCCCATGGAG 
AACCAAACTG TCTTGCTAAC TGCCCCCTGG GGACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306889.1
CDS	159..4937
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_024451121.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451121.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
CAGAATGGTG GAATGGAGCG CCCTGGGAAC TGCAGGCCCG CAACCAAAAC CCAAAGGAAG 
CTGGCGCCCC GGCGCAAGGA TGACAGTGCC GATTTGAGGT GCCAGCTCCA GTTTGCCAAA 
GAGGAAGCCT TCCTGATGCG CAAAAAGATG GCCAAGCTAG GAAGGGAGAA GGACGAGCTG 
GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC TATGGGGATG TGGACAGTCC CCTGCCCACG 
GGGGAAGCAG GCGGGCCCCC CAGCACCCGG GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG 
GTGGAGGAGG AAGCCAACAT CTTGGGCCGG AAGATCGTGG AGCTGGAGGT GGAGAACCGT 
GGCCTCAAGG CAGAGATGGA GGACATGCGG GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG 
GACCACGCAC CCAGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCCACTGAG 
CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG GAAGCGGAGT TGCTCCGGAG GTCCATCTCC 
GAGATCGAAG ACCACAACCG GCAACTGACC CACGAGCTCA GCAAGTTTAA GTTTGAGCCT 
CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC 
CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG CAGATCAGCG AGCTCAGCGG CAAGGTGCTC 
AAACTGCAGC ACGAGAACCA CGCGCTGCTG TCCAACATCC AGCGCTGCGA CCTGGCAGCC 
CACCTGGGGC TGCGTGCCCC CAGTCCCCGG GACAGCGATG CCGAGAGTGA TGCGGGCAAG 
AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC 
GGGGAGAGTG ACTCGGAGGA AATGTTTGAG AAGACGTCGG GCTTCGGGAG CGGGAAGCCA 
TCGGAGGCCA GCGAGCCATG CCCCACGGAG CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC 
CTAGTGACCC TAAAACACGA GGCCCAGCGG CTAGAGCGGA CGGTGGAGCG CCTCATCACG 
GACACCGACA GCTTCCTCCA TGATGCGGGG CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT 
GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG GGGGACCAGC AGGAGCCCCA GCTGCTGGGG 
ACCATCAACG CCAAGATGAA GGCTTTCAAG AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGCATTG GGGATGGCCT ATCCCCCTTG CCCCACCTCA CAGAGTCCTC TAGCTTCCTC 
TCCACTGTGA CTTCCGTGTC CCGGGACTCC CCCATCGGGA ACCTGGGGAA GGAGCTGGGC 
CCAGACTTGC AGTCCAGACT GAAAGAGCAG CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG 
GACGAGCGGG AGAGCCTGCG CCTCCGAGCC GCGCGGGAGC TGCACCGCCG CGCAGACGGG 
GACACCGGGA GCCACGGGCT GGGAGGCCAG ACCTGCTTCA GCCTGGAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGCTGGAAGA GAAGACTGAG 
AACAAGTTGG GAGAACTAGG CTCCTCCGCT GAGAGCAAGG GGGCCTTGAA GAAGGAGAGA 
GAGGTGCACC AGAAGCTCCT GGCAGACAGT CACAGCCTGG TCATGGACCT GCGCTGGCAG 
ATCCATCACA GCGAGAAGAA CTGGAACCGG GAGAAGGTGG AACTTCTCGA CCGCCTGGAC 
AGAGATCGGC AGGAGTGGGA GCGGCAGAAG AAGGAATTCT TGTGGAGGAT AGAGCAGTTG 
CAGAAAGAGA ACAGTCCCCG GAGAGGTGGC AGTTTCCTCT GTGATCAAAA AGACGGCAAC 
GTTCGCCCCT TTCCCCACCA GGGAAGCCTC CGCATGCCCC GTCCAGTGGC CATGTGGCCT 
TGTGCAGATG CTGACTCCAT CCCGTTTGAA GACCGGCCGC TGTCCAAGCT GAAGGAGTCG 
GACAGGTGCT CGGCCAGTGA GAATCTCTAC CTGGATGCCT TGTCCCTGGA TGACGAGCCA 
GAAGAGCCAC CAGCCCACAG GCCCGAGAGG GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA 
GAAAATCACA AAGGAAATCT TCAAAGGGCG GTGTCCGTGT CCTCCATGTC TGAGTTCCAG 
CGTCTAATGG ACATCTCCCC CTTCCTGCCT GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG 
GAGGATGTCA CCCCACCCCT GTCTCCAGAC GACCTCAAGT ACATCGAGGA GTTCAACAAG 
AGCTGGGACT ACACACCCAA CAGGGGCCAC AATGGTGGGG GGCCGGACCT TTGGGCCGAC 
AGGACCGAGG TGGGGCGGGC AGGGCACGAG GACAGCACAG AGCCTTTCCC CGACTCCTCC 
TGGTACCTAA CCACAAGTGT CACCATGACC ACGGACACCA TGACCAGCCC AGAGCACTGC 
CAGAAGCAGC CACTGCGGAG CCACGTCCTC ACCGAGCAGT CGGGGTTGCG CGTGTTACAC 
AGCCCGCCTG CCGTGCGCAG GGTCGACAGC ATCACGGCGG CAGGTGGTGA GGGTCCCTTT 
CCCACAAGCA GAGCCAGAGG GAGCCCGGGA GACACCAAGG GAGGCCCTCC AGAACCCATG 
CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG CACTCCCGGG ACTATGTGGA GGGGGCACGG 
CGCCCCCTTG ATAGTCCCCT CTGTACCTCC CTGGGGTTTG CCTCCCCACT GCACAGCCTG 
GAGATGTCCA AGAACTTGAG TGATGACATG AAGGAGGTGG CCTTCTCTGT CAGGAATGCC 
ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC AAGGACATGG CCTGCCAGAC CAATGGGTCC 
CGGACGATGG GGACCCAGAC TGTTCAGACC ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG 
CGTGGCAGCG GTGTCACCAG CAGCCCCCAC AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT 
GCTACACCCG TGTCGTCTCC TTCCCGGAGC CTTAGGAGCA GACAGGTGGC CCCTGCCATC 
GAGAAGGTGC AGGCCAAGTT TGAACGCACA TGCTGCTCCC CCAAGTATGG TTCTCCCAAG 
CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC CAGCCAAATA ACAGGACGTC ACCAGGGATG 
GCCCAGAAAG GGTACAGTGA GTCAGCCTGG GCCCGCTCCA CCACCACAAG GGAGAGCCCC 
GTGCACACCA CCATTAATGA TGGCCTCTCC AGCCTCTTCA ACATCATTGA CCACAGCCCC 
GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG 
CCCCGACCAG AGCTGGGCCC AGGCCAGGAA ACAGGCACCA ATTCCCGAGG AAGGTCGCCT 
AGCCCCATTG GGGTGGGGTC AGAGATGTGC AGGGAGGAAG GGGGAGAGGG CACGCCAGTG 
AAGCAGGACT TATCTGCTCC CCCTGGCTAC ACGCTCACTG AGAACGTGGC CCGGATCCTC 
AACAAGAAGC TGCTGGAACA TGCCTTAAAG GAGGAGAGGA GGCAGGCTGC CCACGGGCCC 
CCGGGTCTCC ACAGTGACAG CCACTCGCTG GGGGACACAG CCGAGCCAGG GCCCATGGAG 
AACCAAACTG TCTTGCTAAC TGCCCCCTGG GGACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005258099.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258156.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu104528
Clone ID Related Accession (Same CDS sequence)	XM_005258099.5
Accession Version	XM_005258099.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258099.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258156.2
CDS	625..5325
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_005258099.5
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258099.5

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTAGATGA ACTCCGTGCT 
GAGATGGAAG AGATGAGAGA CAGTTATTTA GAGGAAGATG TTTACCAGCT GCAGGAACTT 
CGGCGAGAAC TGGACCGCGC TAATAAAAAC TGCCGAATCC TGCAGTACCG TCTTCGGAAA 
GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG ACGGGTCAGG TGGATGGTGA GCTTATTCGA 
AGCCTGGAGC AGGACTTGAA GGTAGCCAAA GATGTATCTG TCAGATTGCA CCACGAACTT 
AAGACGGTGG AGGAAAAGCG CGCTAAAGCT GAGGATGAAA ACGAAACTCT CCGACAGCAG 
ATGATTGAAG TGGAAATATC CAAACAGGCC CTCCAGAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTAA AAAGAAGAAG CACTCGGGAA ATGTACAAGG AGAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAAGC CTTCCTGATG 
CGCAAAAAGA TGGCCAAGCT AGGAAGGGAG AAGGACGAGC TGGAGCAGGA GCTCCAGAAG 
TACAAGTCCC TCTATGGGGA TGTGGACAGT CCCCTGCCCA CGGGGGAAGC AGGCGGGCCC 
CCCAGCACCC GGGAGGCCGA GCTGAAGCTG CGGCTAAAGC TGGTGGAGGA GGAAGCCAAC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAACC GTGGCCTCAA GGCAGAGATG 
GAGGACATGC GGGGCCAGCA GGAGCGGGAG GGCCCGGGTC GGGACCACGC ACCCAGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCCACTG AGCTCCGCCG CCACCTGCAG 
TTTGTAGAAG AGGAAGCGGA GTTGCTCCGG AGGTCCATCT CCGAGATCGA AGACCACAAC 
CGGCAACTGA CCCACGAGCT CAGCAAGTTT AAGTTTGAGC CTCCCCGGGA GCCGGGCTGG 
CTAGGAGAGG GTGCAAGTCC TGGTGCCGGG GGTGGGGCCC CCCTGCAGGA GGAGCTGAAG 
TCAGCCAGGC TGCAGATCAG CGAGCTCAGC GGCAAGGTGC TCAAACTGCA GCACGAGAAC 
CACGCGCTGC TGTCCAACAT CCAGCGCTGC GACCTGGCAG CCCACCTGGG GCTGCGTGCC 
CCCAGTCCCC GGGACAGCGA TGCCGAGAGT GATGCGGGCA AGAAGGAGAG TGATGGGGAG 
GAGAGCCGCC TGCCCCAGCC CAAGCGGGAA GGGCCTGTTG GCGGGGAGAG TGACTCGGAG 
GAAATGTTTG AGAAGACGTC GGGCTTCGGG AGCGGGAAGC CATCGGAGGC CAGCGAGCCA 
TGCCCCACGG AGCTCCTGAA GGCCCGGGAG GACTCTGAGT ACCTAGTGAC CCTAAAACAC 
GAGGCCCAGC GGCTAGAGCG GACGGTGGAG CGCCTCATCA CGGACACCGA CAGCTTCCTC 
CATGATGCGG GGCTGCGGGG TGGTGCGCCC TTACCGGGGC CTGGCCTCCA GGGCGAAGAG 
GAGCAGGGTG AGGGGGACCA GCAGGAGCCC CAGCTGCTGG GGACCATCAA CGCCAAGATG 
AAGGCTTTCA AGAAAGAGCT GCAGGCCTTC CTGGAGCAGG TGAACCGCAT TGGGGATGGC 
CTATCCCCCT TGCCCCACCT CACAGAGTCC TCTAGCTTCC TCTCCACTGT GACTTCCGTG 
TCCCGGGACT CCCCCATCGG GAACCTGGGG AAGGAGCTGG GCCCAGACTT GCAGTCCAGA 
CTGAAAGAGC AGCTGGAGTG GCAGCTCGGG CCGGCCCGAG GGGACGAGCG GGAGAGCCTG 
CGCCTCCGAG CCGCGCGGGA GCTGCACCGC CGCGCAGACG GGGACACCGG GAGCCACGGG 
CTGGGAGGCC AGACCTGCTT CAGCCTGGAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451123.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306891.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu104529
Clone ID Related Accession (Same CDS sequence)	XM_024451123.1
Accession Version	XM_024451123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GAATTGTTGT GGATATCCCA GAATTAACAT TGAGGAGGAG 
ACTTTAGGCT TCACCAGGCT GCCAGCTGGG TCCACGGTAA AAACGTTGAA GAGCCTTGGG 
TTGCAGAGAT TGGAGCTGGA AGAGAAGACT GAGAACAAGT TGGGAGAACT AGGCTCCTCC 
GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC 
AGTCACAGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGCGAGAA GAACTGGAAC 
CGGGAGAAGG TGGAACTTCT CGACCGCCTG GACAGAGATC GGCAGGAGTG GGAGCGGCAG 
AAGAAGGAAT TCTTGTGGAG GATAGAGCAG TTGCAGAAAG AGAACAGTCC CCGGAGAGGT 
GGCAGTTTCC TCTGTGATCA AAAAGACGGC AACGTTCGCC CCTTTCCCCA CCAGGGAAGC 
CTCCGCATGC CCCGTCCAGT GGCCATGTGG CCTTGTGCAG ATGCTGACTC CATCCCGTTT 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAG TCGGACAGGT GCTCGGCCAG TGAGAATCTC 
TACCTGGATG CCTTGTCCCT GGATGACGAG CCAGAAGAGC CACCAGCCCA CAGGCCCGAG 
AGGGAGTTCA GGAACCGCCT CCCTGAGGAA GAAGAAAATC ACAAAGGAAA TCTTCAAAGG 
GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC CAGCGTCTAA TGGACATCTC CCCCTTCCTG 
CCTGAGAAGG GCCTGCCGTC CACCAGCAGC AAGGAGGATG TCACCCCACC CCTGTCTCCA 
GACGACCTCA AGTACATCGA GGAGTTCAAC AAGAGCTGGG ACTACACACC CAACAGGGGC 
CACAATGGTG GGGGGCCGGA CCTTTGGGCC GACAGGACCG AGGTGGGGCG GGCAGGGCAC 
GAGGACAGCA CAGAGCCTTT CCCCGACTCC TCCTGGTACC TAACCACAAG TGTCACCATG 
ACCACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCACTGCG GAGCCACGTC 
CTCACCGAGC AGTCGGGGTT GCGCGTGTTA CACAGCCCGC CTGCCGTGCG CAGGGTCGAC 
AGCATCACGG CGGCAGGTGG TGAGGGTCCC TTTCCCACAA GCAGAGCCAG AGGGAGCCCG 
GGAGACACCA AGGGAGGCCC TCCAGAACCC ATGCTCAGCA GGTGGCCTTG CACCTCCCCC 
AGGCACTCCC GGGACTATGT GGAGGGGGCA CGGCGCCCCC TTGATAGTCC CCTCTGTACC 
TCCCTGGGGT TTGCCTCCCC ACTGCACAGC CTGGAGATGT CCAAGAACTT GAGTGATGAC 
ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT GCCATCTGCT CCGGCCCTGG CGAGCTGCAA 
GTCAAGGACA TGGCCTGCCA GACCAATGGG TCCCGGACGA TGGGGACCCA GACTGTTCAG 
ACCATCAGTG TGGGCTTGCA GACTGAAGCC CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC 
CACAAGTGTC TCACTCCAAA GGCTGGGGGC GGTGCTACAC CCGTGTCGTC TCCTTCCCGG 
AGCCTTAGGA GCAGACAGGT GGCCCCTGCC ATCGAGAAGG TGCAGGCCAA GTTTGAACGC 
ACATGCTGCT CCCCCAAGTA TGGTTCTCCC AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC 
GACCAGCCAA ATAACAGGAC GTCACCAGGG ATGGCCCAGA AAGGGTACAG TGAGTCAGCC 
TGGGCCCGCT CCACCACCAC AAGGGAGAGC CCCGTGCACA CCACCATTAA TGATGGCCTC 
TCCAGCCTCT TCAACATCAT TGACCACAGC CCCGTGGTGC AGGACCCCTT CCAGAAGGGG 
CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG 
GAAACAGGCA CCAATTCCCG AGGAAGGTCG CCTAGCCCCA TTGGGGTGGG GTCAGAGATG 
TGCAGGGAGG AAGGGGGAGA GGGCACGCCA GTGAAGCAGG ACTTATCTGC TCCCCCTGGC 
TACACGCTCA CTGAGAACGT GGCCCGGATC CTCAACAAGA AGCTGCTGGA ACATGCCTTA 
AAGGAGGAGA GGAGGCAGGC TGCCCACGGG CCCCCGGGTC TCCACAGTGA CAGCCACTCG 
CTGGGGGACA CAGCCGAGCC AGGGCCCATG GAGGAACTAC CTTGTTCTGC ACTAGCTCCA 
TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGT 
TGGGCCCCAC ATTCCCCCAC TGCCTCACAG CCTCAGTCAC CCGGAGACCC GACGTCCTTG 
GAGGAGCATG GTGGCGAGGA GCCGCCCGAG GAGCAGCCAC ACCGAGATGC AAGCTTGCAT 
GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306891.1
CDS	79..5025
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451123.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451123.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GAATTGTTGT GGATATCCCA GAATTAACAT TGAGGAGGAG 
ACTTTAGGCT TCACCAGGCT GCCAGCTGGG TCCACGGTAA AAACGTTGAA GAGCCTTGGG 
TTGCAGAGAT TGGAGCTGGA AGAGAAGACT GAGAACAAGT TGGGAGAACT AGGCTCCTCC 
GCTGAGAGCA AGGGGGCCTT GAAGAAGGAG AGAGAGGTGC ACCAGAAGCT CCTGGCAGAC 
AGTCACAGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGCGAGAA GAACTGGAAC 
CGGGAGAAGG TGGAACTTCT CGACCGCCTG GACAGAGATC GGCAGGAGTG GGAGCGGCAG 
AAGAAGGAAT TCTTGTGGAG GATAGAGCAG TTGCAGAAAG AGAACAGTCC CCGGAGAGGT 
GGCAGTTTCC TCTGTGATCA AAAAGACGGC AACGTTCGCC CCTTTCCCCA CCAGGGAAGC 
CTCCGCATGC CCCGTCCAGT GGCCATGTGG CCTTGTGCAG ATGCTGACTC CATCCCGTTT 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAG TCGGACAGGT GCTCGGCCAG TGAGAATCTC 
TACCTGGATG CCTTGTCCCT GGATGACGAG CCAGAAGAGC CACCAGCCCA CAGGCCCGAG 
AGGGAGTTCA GGAACCGCCT CCCTGAGGAA GAAGAAAATC ACAAAGGAAA TCTTCAAAGG 
GCGGTGTCCG TGTCCTCCAT GTCTGAGTTC CAGCGTCTAA TGGACATCTC CCCCTTCCTG 
CCTGAGAAGG GCCTGCCGTC CACCAGCAGC AAGGAGGATG TCACCCCACC CCTGTCTCCA 
GACGACCTCA AGTACATCGA GGAGTTCAAC AAGAGCTGGG ACTACACACC CAACAGGGGC 
CACAATGGTG GGGGGCCGGA CCTTTGGGCC GACAGGACCG AGGTGGGGCG GGCAGGGCAC 
GAGGACAGCA CAGAGCCTTT CCCCGACTCC TCCTGGTACC TAACCACAAG TGTCACCATG 
ACCACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCACTGCG GAGCCACGTC 
CTCACCGAGC AGTCGGGGTT GCGCGTGTTA CACAGCCCGC CTGCCGTGCG CAGGGTCGAC 
AGCATCACGG CGGCAGGTGG TGAGGGTCCC TTTCCCACAA GCAGAGCCAG AGGGAGCCCG 
GGAGACACCA AGGGAGGCCC TCCAGAACCC ATGCTCAGCA GGTGGCCTTG CACCTCCCCC 
AGGCACTCCC GGGACTATGT GGAGGGGGCA CGGCGCCCCC TTGATAGTCC CCTCTGTACC 
TCCCTGGGGT TTGCCTCCCC ACTGCACAGC CTGGAGATGT CCAAGAACTT GAGTGATGAC 
ATGAAGGAGG TGGCCTTCTC TGTCAGGAAT GCCATCTGCT CCGGCCCTGG CGAGCTGCAA 
GTCAAGGACA TGGCCTGCCA GACCAATGGG TCCCGGACGA TGGGGACCCA GACTGTTCAG 
ACCATCAGTG TGGGCTTGCA GACTGAAGCC CTGCGTGGCA GCGGTGTCAC CAGCAGCCCC 
CACAAGTGTC TCACTCCAAA GGCTGGGGGC GGTGCTACAC CCGTGTCGTC TCCTTCCCGG 
AGCCTTAGGA GCAGACAGGT GGCCCCTGCC ATCGAGAAGG TGCAGGCCAA GTTTGAACGC 
ACATGCTGCT CCCCCAAGTA TGGTTCTCCC AAGCTGCAGA GGAAGCCCCT CCCCAAAGCC 
GACCAGCCAA ATAACAGGAC GTCACCAGGG ATGGCCCAGA AAGGGTACAG TGAGTCAGCC 
TGGGCCCGCT CCACCACCAC AAGGGAGAGC CCCGTGCACA CCACCATTAA TGATGGCCTC 
TCCAGCCTCT TCAACATCAT TGACCACAGC CCCGTGGTGC AGGACCCCTT CCAGAAGGGG 
CTGCGGGCCG GCAGTCGGTC TCGCTCAGCA GAGCCCCGAC CAGAGCTGGG CCCAGGCCAG 
GAAACAGGCA CCAATTCCCG AGGAAGGTCG CCTAGCCCCA TTGGGGTGGG GTCAGAGATG 
TGCAGGGAGG AAGGGGGAGA GGGCACGCCA GTGAAGCAGG ACTTATCTGC TCCCCCTGGC 
TACACGCTCA CTGAGAACGT GGCCCGGATC CTCAACAAGA AGCTGCTGGA ACATGCCTTA 
AAGGAGGAGA GGAGGCAGGC TGCCCACGGG CCCCCGGGTC TCCACAGTGA CAGCCACTCG 
CTGGGGGACA CAGCCGAGCC AGGGCCCATG GAGGAACTAC CTTGTTCTGC ACTAGCTCCA 
TCCCTAGAGC CCTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGT 
TGGGCCCCAC ATTCCCCCAC TGCCTCACAG CCTCAGTCAC CCGGAGACCC GACGTCCTTG 
GAGGAGCATG GTGGCGAGGA GCCGCCCGAG GAGCAGCCAC ACCGAGATGC AAGCTTGCAT 
GGATTATCAC AGTATAATTC ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025671.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881160.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

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CloneID	OHu87828
Clone ID Related Accession (Same CDS sequence)	XM_017025671.1 , XM_017025671.2
Accession Version	XM_017025671.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTGG AGGGCCCCCG GCTACGGACC CGCTTGCCCC TTCTCCTGCA ATTACGGAAA 
GAGCTGGGGG CTCTCAGGGT CTCCTGGGCT CCGACATCCC GGGTAGAGGG GGCCGCGCCG 
TTTTCGGATG AGTTAGATGA ACTCCGTGCT GAGATGGAAG AGATGAGAGA CAGTTATTTA 
GAGGAAGATG TTTACCAGCT GCAGGAACTT CGGCGAGAAC TGGACCGCGC TAATAAAAAC 
TGCCGAATCC TGCAGTACCG TCTTCGGAAA GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG 
ACGGGTCAGG TGGATGGTGA GCTTATTCGA AGCCTGGAGC AGGACTTGAA GGTAGCCAAA 
GATGTATCTG TCAGATTGCA CCACGAACTT AAGACGGTGG AGGAAAAGCG CGCTAAAGCT 
GAGGATGAAA ACGAAACTCT CCGACAGCAG ATGATTGAAG TGGAAATATC CAAACAGGCC 
CTCCAGAATG AGCTGGAGAG ACTGAAAGAG AGTTCCCTAA AAAGAAGAAG CACTCGGGAA 
ATGTACAAGG AGAAGAAAAC CTTTAACCAG CAGAATGGTG GAATGGAGCG CCCTGGGAAC 
TGCAGGCCCG CAACCAAAAC CCAAAGGAAG CTGGCGCCCC GGCGCAAGGA TGACAGTGCC 
GATTTGAGGT GCCAGCTCCA GTTTGCCAAA GAGGAAGCCT TCCTGATGCG CAAAAAGATG 
GCCAAGCTAG GAAGGGAGAA GGACGAGCTG GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC 
TATGGGGATG TGGACAGTCC CCTGCCCACG GGGGAAGCAG GCGGGCCCCC CAGCACCCGG 
GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG GTGGAGGAGG AAGCCAACAT CTTGGGCCGG 
AAGATCGTGG AGCTGGAGGT GGAGAACCGT GGCCTCAAGG CAGAGATGGA GGACATGCGG 
GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG GACCACGCAC CCAGCATTCC TACCTCACCC 
TTCGGTGACT CCCTGGAGTC CTCCACTGAG CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG 
GAAGCGGAGT TGCTCCGGAG GTCCATCTCC GAGATCGAAG ACCACAACCG GCAACTGACC 
CACGAGCTCA GCAAGTTTAA GTTTGAGCCT CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT 
GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG 
CAGATCAGCG AGCTCAGCGG CAAGGTGCTC AAACTGCAGC ACGAGAACCA CGCGCTGCTG 
TCCAACATCC AGCGCTGCGA CCTGGCAGCC CACCTGGGGC TGCGTGCCCC CAGTCCCCGG 
GACAGCGATG CCGAGAGTGA TGCGGGCAAG AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG 
CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC GGGGAGAGTG ACTCGGAGGA AATGTTTGAG 
AAGACGTCGG GCTTCGGGAG CGGGAAGCCA TCGGAGGCCA GCGAGCCATG CCCCACGGAG 
CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC CTAGTGACCC TAAAACACGA GGCCCAGCGG 
CTAGAGCGGA CGGTGGAGCG CCTCATCACG GACACCGACA GCTTCCTCCA TGATGCGGGG 
CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG 
GGGGACCAGC AGGAGCCCCA GCTGCTGGGG ACCATCAACG CCAAGATGAA GGCTTTCAAG 
AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG AACCGCATTG GGGATGGCCT ATCCCCCTTG 
CCCCACCTCA CAGAGTCCTC TAGCTTCCTC TCCACTGTGA CTTCCGTGTC CCGGGACTCC 
CCCATCGGGA ACCTGGGGAA GGAGCTGGGC CCAGACTTGC AGTCCAGACT GAAAGAGCAG 
CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG GACGAGCGGG AGAGCCTGCG CCTCCGAGCC 
GCGCGGGAGC TGCACCGCCG CGCAGACGGG GACACCGGGA GCCACGGGCT GGGAGGCCAG 
ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC CCCGTTTTAC CTGAGCAGAG TGTATCCATA 
GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG GCCAGACTGC ATCAAGAGGA GACAGAGACA 
TTTACAAACA AGATCCATAA GATGGAGGAG GAGCACCTCT ATGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAAAAC ACCCTCCATG AGCGAACCTG GAGTGATGAG 
AAGAATCTGA TGCAGCAGGA GCTCCGGTCC TTGAAGCAGA ACATTTTCCT CTTCTACGTC 
AAACTCAGGT GGCTGCTGAA ACACTGGCGG CAAGGGAAGC AGATGGAGGA GGAAGGAGAG 
GAGTTCACTG AGGGTGAACA TCCAGAGACC CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG 
GGGGGTCACC AGGCGGATGG CCCAGACCAC GACAGTGACC GAGGCTGTGG CTTTCCAGTG 
GGGGAGCACT CCCCACACTC CCGGGTGCAG ATTGGAGATC ACAGCTTGCG GCTGCAGACC 
GCGGACAGGG GACAGCCCCA CAAACAGGTG GTGGAAAACC AGCAGCTGTT CAGCGCCTTC 
AAGGCCTTGC TGGAGGACTT CCGTGCGGAG CTGCGGGAGG ATGAGCGTGC CCGACTACGG 
CTGCAGCAGC AATATGCCAG CGACAAGGCG GCCTGGGACG TGGAGTGGGC CGTGCTCAAG 
TGCCGTCTGG AACAGAATTG TTGTGGATAT CCCAGAATTA ACATTGAGGA GGAGACTTTA 
GGCTTCACCA GGCTGCCAGC TGGGTCCACG GTAAAAACGT TGAAGAGCCT TGGGTTGCAG 
AGATTGGAGC TGGAAGAGAA GACTGAGAAC AAGTTGGGAG AACTAGGCTC CTCCGCTGAG 
AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG GTGCACCAGA AGCTCCTGGC AGACAGTCAC 
AGCCTGGTCA TGGACCTGCG CTGGCAGATC CATCACAGCG AGAAGAACTG GAACCGGGAG 
AAGGTGGAAC TTCTCGACCG CCTGGACAGA GATCGGCAGG AGTGGGAGCG GCAGAAGAAG 
GAATTCTTGT GGAGGATAGA GCAGTTGCAG AAAGAGAACA GTCCCCGGAG AGGTGGCAGT 
TTCCTCTGTG ATCAAAAAGA CGGCAACGTT CGCCCCTTTC CCCACCAGGG AAGCCTCCGC 
ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT GCAGATGCTG ACTCCATCCC GTTTGAAGAC 
CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC AGGTGCTCGG CCAGTGAGAA TCTCTACCTG 
GATGCCTTGT CCCTGGATGA CGAGCCAGAA GAGCCACCAG CCCACAGGCC CGAGAGGGAG 
TTCAGGAACC GCCTCCCTGA GGAAGAAGAA AATCACAAAG GAAATCTTCA AAGGGCGGTG 
TCCGTGTCCT CCATGTCTGA GTTCCAGCGT CTAATGGACA TCTCCCCCTT CCTGCCTGAG 
AAGGGCCTGC CGTCCACCAG CAGCAAGGAG GATGTCACCC CACCCCTGTC TCCAGACGAC 
CTCAAGTACA TCGAGGAGTT CAACAAGAGC TGGGACTACA CACCCAACAG GGGCCACAAT 
GGTGGGGGGC CGGACCTTTG GGCCGACAGG ACCGAGGTGG GGCGGGCAGG GCACGAGGAC 
AGCACAGAGC CTTTCCCCGA CTCCTCCTGG TACCTAACCA CAAGTGTCAC CATGACCACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCAC TGCGGAGCCA CGTCCTCACC 
GAGCAGTCGG GGTTGCGCGT GTTACACAGC CCGCCTGCCG TGCGCAGGGT CGACAGCATC 
ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC 
ACCAAGGGAG GCCCTCCAGA ACCCATGCTC AGCAGGTGGC CTTGCACCTC CCCCAGGCAC 
TCCCGGGACT ATGTGGAGGG GGCACGGCGC CCCCTTGATA GTCCCCTCTG TACCTCCCTG 
GGGTTTGCCT CCCCACTGCA CAGCCTGGAG ATGTCCAAGA ACTTGAGTGA TGACATGAAG 
GAGGTGGCCT TCTCTGTCAG GAATGCCATC TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACGATGGGGA CCCAGACTGT TCAGACCATC 
AGTGTGGGCT TGCAGACTGA AGCCCTGCGT GGCAGCGGTG TCACCAGCAG CCCCCACAAG 
TGTCTCACTC CAAAGGCTGG GGGCGGTGCT ACACCCGTGT CGTCTCCTTC CCGGAGCCTT 
AGGAGCAGAC AGGTGGCCCC TGCCATCGAG AAGGTGCAGG CCAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTATGGTTC TCCCAAGCTG CAGAGGAAGC CCCTCCCCAA AGCCGACCAG 
CCAAATAACA GGACGTCACC AGGGATGGCC CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC 
CGCTCCACCA CCACAAGGGA GAGCCCCGTG CACACCACCA TTAATGATGG CCTCTCCAGC 
CTCTTCAACA TCATTGACCA CAGCCCCGTG GTGCAGGACC CCTTCCAGAA GGGGCTGCGG 
GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC CGACCAGAGC TGGGCCCAGG CCAGGAAACA 
GGCACCAATT CCCGAGGAAG GTCGCCTAGC CCCATTGGGG TGGGGTCAGA GATGTGCAGG 
GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG CAGGACTTAT CTGCTCCCCC TGGCTACACG 
CTCACTGAGA ACGTGGCCCG GATCCTCAAC AAGAAGCTGC TGGAACATGC CTTAAAGGAG 
GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG GGTCTCCACA GTGACAGCCA CTCGCTGGGG 
GACACAGCCG AGCCAGGGCC CATGGAGGAA CTACCTTGTT CTGCACTAGC TCCATCCCTA 
GAGCCCTGCT TCTCCAGGCC CGAGAGACCA GCAAACCGTC GCCCTCCGTC CCGTTGGGCC 
CCACATTCCC CCACTGCCTC ACAGCCTCAG TCACCCGGAG ACCCGACGTC CTTGGAGGAG 
CATGGTGGCG AGGAGCCGCC CGAGGAGCAG CCACACCGAG ATGCAAGCTT GCATGGATTA 
TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881160.1
CDS	382..5682
Translation	MPLEGPRLRTRLPLLLQLRKELGALRVSWAPTSRVEGAAPFSDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025671.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025671.1

ORF Insert Sequence:

ATGCCACTGG AGGGCCCCCG GCTACGGACC CGCTTGCCCC TTCTCCTGCA ATTACGGAAA 
GAGCTGGGGG CTCTCAGGGT CTCCTGGGCT CCGACATCCC GGGTAGAGGG GGCCGCGCCG 
TTTTCGGATG AGTTAGATGA ACTCCGTGCT GAGATGGAAG AGATGAGAGA CAGTTATTTA 
GAGGAAGATG TTTACCAGCT GCAGGAACTT CGGCGAGAAC TGGACCGCGC TAATAAAAAC 
TGCCGAATCC TGCAGTACCG TCTTCGGAAA GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG 
ACGGGTCAGG TGGATGGTGA GCTTATTCGA AGCCTGGAGC AGGACTTGAA GGTAGCCAAA 
GATGTATCTG TCAGATTGCA CCACGAACTT AAGACGGTGG AGGAAAAGCG CGCTAAAGCT 
GAGGATGAAA ACGAAACTCT CCGACAGCAG ATGATTGAAG TGGAAATATC CAAACAGGCC 
CTCCAGAATG AGCTGGAGAG ACTGAAAGAG AGTTCCCTAA AAAGAAGAAG CACTCGGGAA 
ATGTACAAGG AGAAGAAAAC CTTTAACCAG CAGAATGGTG GAATGGAGCG CCCTGGGAAC 
TGCAGGCCCG CAACCAAAAC CCAAAGGAAG CTGGCGCCCC GGCGCAAGGA TGACAGTGCC 
GATTTGAGGT GCCAGCTCCA GTTTGCCAAA GAGGAAGCCT TCCTGATGCG CAAAAAGATG 
GCCAAGCTAG GAAGGGAGAA GGACGAGCTG GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC 
TATGGGGATG TGGACAGTCC CCTGCCCACG GGGGAAGCAG GCGGGCCCCC CAGCACCCGG 
GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG GTGGAGGAGG AAGCCAACAT CTTGGGCCGG 
AAGATCGTGG AGCTGGAGGT GGAGAACCGT GGCCTCAAGG CAGAGATGGA GGACATGCGG 
GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG GACCACGCAC CCAGCATTCC TACCTCACCC 
TTCGGTGACT CCCTGGAGTC CTCCACTGAG CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG 
GAAGCGGAGT TGCTCCGGAG GTCCATCTCC GAGATCGAAG ACCACAACCG GCAACTGACC 
CACGAGCTCA GCAAGTTTAA GTTTGAGCCT CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT 
GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG 
CAGATCAGCG AGCTCAGCGG CAAGGTGCTC AAACTGCAGC ACGAGAACCA CGCGCTGCTG 
TCCAACATCC AGCGCTGCGA CCTGGCAGCC CACCTGGGGC TGCGTGCCCC CAGTCCCCGG 
GACAGCGATG CCGAGAGTGA TGCGGGCAAG AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG 
CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC GGGGAGAGTG ACTCGGAGGA AATGTTTGAG 
AAGACGTCGG GCTTCGGGAG CGGGAAGCCA TCGGAGGCCA GCGAGCCATG CCCCACGGAG 
CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC CTAGTGACCC TAAAACACGA GGCCCAGCGG 
CTAGAGCGGA CGGTGGAGCG CCTCATCACG GACACCGACA GCTTCCTCCA TGATGCGGGG 
CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG 
GGGGACCAGC AGGAGCCCCA GCTGCTGGGG ACCATCAACG CCAAGATGAA GGCTTTCAAG 
AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG AACCGCATTG GGGATGGCCT ATCCCCCTTG 
CCCCACCTCA CAGAGTCCTC TAGCTTCCTC TCCACTGTGA CTTCCGTGTC CCGGGACTCC 
CCCATCGGGA ACCTGGGGAA GGAGCTGGGC CCAGACTTGC AGTCCAGACT GAAAGAGCAG 
CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG GACGAGCGGG AGAGCCTGCG CCTCCGAGCC 
GCGCGGGAGC TGCACCGCCG CGCAGACGGG GACACCGGGA GCCACGGGCT GGGAGGCCAG 
ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC CCCGTTTTAC CTGAGCAGAG TGTATCCATA 
GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG GCCAGACTGC ATCAAGAGGA GACAGAGACA 
TTTACAAACA AGATCCATAA GATGGAGGAG GAGCACCTCT ATGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAAAAC ACCCTCCATG AGCGAACCTG GAGTGATGAG 
AAGAATCTGA TGCAGCAGGA GCTCCGGTCC TTGAAGCAGA ACATTTTCCT CTTCTACGTC 
AAACTCAGGT GGCTGCTGAA ACACTGGCGG CAAGGGAAGC AGATGGAGGA GGAAGGAGAG 
GAGTTCACTG AGGGTGAACA TCCAGAGACC CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG 
GGGGGTCACC AGGCGGATGG CCCAGACCAC GACAGTGACC GAGGCTGTGG CTTTCCAGTG 
GGGGAGCACT CCCCACACTC CCGGGTGCAG ATTGGAGATC ACAGCTTGCG GCTGCAGACC 
GCGGACAGGG GACAGCCCCA CAAACAGGTG GTGGAAAACC AGCAGCTGTT CAGCGCCTTC 
AAGGCCTTGC TGGAGGACTT CCGTGCGGAG CTGCGGGAGG ATGAGCGTGC CCGACTACGG 
CTGCAGCAGC AATATGCCAG CGACAAGGCG GCCTGGGACG TGGAGTGGGC CGTGCTCAAG 
TGCCGTCTGG AACAGAATTG TTGTGGATAT CCCAGAATTA ACATTGAGGA GGAGACTTTA 
GGCTTCACCA GGCTGCCAGC TGGGTCCACG GTAAAAACGT TGAAGAGCCT TGGGTTGCAG 
AGATTGGAGC TGGAAGAGAA GACTGAGAAC AAGTTGGGAG AACTAGGCTC CTCCGCTGAG 
AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG GTGCACCAGA AGCTCCTGGC AGACAGTCAC 
AGCCTGGTCA TGGACCTGCG CTGGCAGATC CATCACAGCG AGAAGAACTG GAACCGGGAG 
AAGGTGGAAC TTCTCGACCG CCTGGACAGA GATCGGCAGG AGTGGGAGCG GCAGAAGAAG 
GAATTCTTGT GGAGGATAGA GCAGTTGCAG AAAGAGAACA GTCCCCGGAG AGGTGGCAGT 
TTCCTCTGTG ATCAAAAAGA CGGCAACGTT CGCCCCTTTC CCCACCAGGG AAGCCTCCGC 
ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT GCAGATGCTG ACTCCATCCC GTTTGAAGAC 
CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC AGGTGCTCGG CCAGTGAGAA TCTCTACCTG 
GATGCCTTGT CCCTGGATGA CGAGCCAGAA GAGCCACCAG CCCACAGGCC CGAGAGGGAG 
TTCAGGAACC GCCTCCCTGA GGAAGAAGAA AATCACAAAG GAAATCTTCA AAGGGCGGTG 
TCCGTGTCCT CCATGTCTGA GTTCCAGCGT CTAATGGACA TCTCCCCCTT CCTGCCTGAG 
AAGGGCCTGC CGTCCACCAG CAGCAAGGAG GATGTCACCC CACCCCTGTC TCCAGACGAC 
CTCAAGTACA TCGAGGAGTT CAACAAGAGC TGGGACTACA CACCCAACAG GGGCCACAAT 
GGTGGGGGGC CGGACCTTTG GGCCGACAGG ACCGAGGTGG GGCGGGCAGG GCACGAGGAC 
AGCACAGAGC CTTTCCCCGA CTCCTCCTGG TACCTAACCA CAAGTGTCAC CATGACCACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCAC TGCGGAGCCA CGTCCTCACC 
GAGCAGTCGG GGTTGCGCGT GTTACACAGC CCGCCTGCCG TGCGCAGGGT CGACAGCATC 
ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC 
ACCAAGGGAG GCCCTCCAGA ACCCATGCTC AGCAGGTGGC CTTGCACCTC CCCCAGGCAC 
TCCCGGGACT ATGTGGAGGG GGCACGGCGC CCCCTTGATA GTCCCCTCTG TACCTCCCTG 
GGGTTTGCCT CCCCACTGCA CAGCCTGGAG ATGTCCAAGA ACTTGAGTGA TGACATGAAG 
GAGGTGGCCT TCTCTGTCAG GAATGCCATC TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACGATGGGGA CCCAGACTGT TCAGACCATC 
AGTGTGGGCT TGCAGACTGA AGCCCTGCGT GGCAGCGGTG TCACCAGCAG CCCCCACAAG 
TGTCTCACTC CAAAGGCTGG GGGCGGTGCT ACACCCGTGT CGTCTCCTTC CCGGAGCCTT 
AGGAGCAGAC AGGTGGCCCC TGCCATCGAG AAGGTGCAGG CCAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTATGGTTC TCCCAAGCTG CAGAGGAAGC CCCTCCCCAA AGCCGACCAG 
CCAAATAACA GGACGTCACC AGGGATGGCC CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC 
CGCTCCACCA CCACAAGGGA GAGCCCCGTG CACACCACCA TTAATGATGG CCTCTCCAGC 
CTCTTCAACA TCATTGACCA CAGCCCCGTG GTGCAGGACC CCTTCCAGAA GGGGCTGCGG 
GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC CGACCAGAGC TGGGCCCAGG CCAGGAAACA 
GGCACCAATT CCCGAGGAAG GTCGCCTAGC CCCATTGGGG TGGGGTCAGA GATGTGCAGG 
GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG CAGGACTTAT CTGCTCCCCC TGGCTACACG 
CTCACTGAGA ACGTGGCCCG GATCCTCAAC AAGAAGCTGC TGGAACATGC CTTAAAGGAG 
GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG GGTCTCCACA GTGACAGCCA CTCGCTGGGG 
GACACAGCCG AGCCAGGGCC CATGGAGGAA CTACCTTGTT CTGCACTAGC TCCATCCCTA 
GAGCCCTGCT TCTCCAGGCC CGAGAGACCA GCAAACCGTC GCCCTCCGTC CCGTTGGGCC 
CCACATTCCC CCACTGCCTC ACAGCCTCAG TCACCCGGAG ACCCGACGTC CTTGGAGGAG 
CATGGTGGCG AGGAGCCGCC CGAGGAGCAG CCACACCGAG ATGCAAGCTT GCATGGATTA 
TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025671.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881160.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X17, mRNA.

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CloneID	OHu87828
Clone ID Related Accession (Same CDS sequence)	XM_017025671.1 , XM_017025671.2
Accession Version	XM_017025671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTGG AGGGCCCCCG GCTACGGACC CGCTTGCCCC TTCTCCTGCA ATTACGGAAA 
GAGCTGGGGG CTCTCAGGGT CTCCTGGGCT CCGACATCCC GGGTAGAGGG GGCCGCGCCG 
TTTTCGGATG AGTTAGATGA ACTCCGTGCT GAGATGGAAG AGATGAGAGA CAGTTATTTA 
GAGGAAGATG TTTACCAGCT GCAGGAACTT CGGCGAGAAC TGGACCGCGC TAATAAAAAC 
TGCCGAATCC TGCAGTACCG TCTTCGGAAA GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG 
ACGGGTCAGG TGGATGGTGA GCTTATTCGA AGCCTGGAGC AGGACTTGAA GGTAGCCAAA 
GATGTATCTG TCAGATTGCA CCACGAACTT AAGACGGTGG AGGAAAAGCG CGCTAAAGCT 
GAGGATGAAA ACGAAACTCT CCGACAGCAG ATGATTGAAG TGGAAATATC CAAACAGGCC 
CTCCAGAATG AGCTGGAGAG ACTGAAAGAG AGTTCCCTAA AAAGAAGAAG CACTCGGGAA 
ATGTACAAGG AGAAGAAAAC CTTTAACCAG CAGAATGGTG GAATGGAGCG CCCTGGGAAC 
TGCAGGCCCG CAACCAAAAC CCAAAGGAAG CTGGCGCCCC GGCGCAAGGA TGACAGTGCC 
GATTTGAGGT GCCAGCTCCA GTTTGCCAAA GAGGAAGCCT TCCTGATGCG CAAAAAGATG 
GCCAAGCTAG GAAGGGAGAA GGACGAGCTG GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC 
TATGGGGATG TGGACAGTCC CCTGCCCACG GGGGAAGCAG GCGGGCCCCC CAGCACCCGG 
GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG GTGGAGGAGG AAGCCAACAT CTTGGGCCGG 
AAGATCGTGG AGCTGGAGGT GGAGAACCGT GGCCTCAAGG CAGAGATGGA GGACATGCGG 
GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG GACCACGCAC CCAGCATTCC TACCTCACCC 
TTCGGTGACT CCCTGGAGTC CTCCACTGAG CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG 
GAAGCGGAGT TGCTCCGGAG GTCCATCTCC GAGATCGAAG ACCACAACCG GCAACTGACC 
CACGAGCTCA GCAAGTTTAA GTTTGAGCCT CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT 
GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG 
CAGATCAGCG AGCTCAGCGG CAAGGTGCTC AAACTGCAGC ACGAGAACCA CGCGCTGCTG 
TCCAACATCC AGCGCTGCGA CCTGGCAGCC CACCTGGGGC TGCGTGCCCC CAGTCCCCGG 
GACAGCGATG CCGAGAGTGA TGCGGGCAAG AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG 
CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC GGGGAGAGTG ACTCGGAGGA AATGTTTGAG 
AAGACGTCGG GCTTCGGGAG CGGGAAGCCA TCGGAGGCCA GCGAGCCATG CCCCACGGAG 
CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC CTAGTGACCC TAAAACACGA GGCCCAGCGG 
CTAGAGCGGA CGGTGGAGCG CCTCATCACG GACACCGACA GCTTCCTCCA TGATGCGGGG 
CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG 
GGGGACCAGC AGGAGCCCCA GCTGCTGGGG ACCATCAACG CCAAGATGAA GGCTTTCAAG 
AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG AACCGCATTG GGGATGGCCT ATCCCCCTTG 
CCCCACCTCA CAGAGTCCTC TAGCTTCCTC TCCACTGTGA CTTCCGTGTC CCGGGACTCC 
CCCATCGGGA ACCTGGGGAA GGAGCTGGGC CCAGACTTGC AGTCCAGACT GAAAGAGCAG 
CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG GACGAGCGGG AGAGCCTGCG CCTCCGAGCC 
GCGCGGGAGC TGCACCGCCG CGCAGACGGG GACACCGGGA GCCACGGGCT GGGAGGCCAG 
ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC CCCGTTTTAC CTGAGCAGAG TGTATCCATA 
GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG GCCAGACTGC ATCAAGAGGA GACAGAGACA 
TTTACAAACA AGATCCATAA GATGGAGGAG GAGCACCTCT ATGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAAAAC ACCCTCCATG AGCGAACCTG GAGTGATGAG 
AAGAATCTGA TGCAGCAGGA GCTCCGGTCC TTGAAGCAGA ACATTTTCCT CTTCTACGTC 
AAACTCAGGT GGCTGCTGAA ACACTGGCGG CAAGGGAAGC AGATGGAGGA GGAAGGAGAG 
GAGTTCACTG AGGGTGAACA TCCAGAGACC CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG 
GGGGGTCACC AGGCGGATGG CCCAGACCAC GACAGTGACC GAGGCTGTGG CTTTCCAGTG 
GGGGAGCACT CCCCACACTC CCGGGTGCAG ATTGGAGATC ACAGCTTGCG GCTGCAGACC 
GCGGACAGGG GACAGCCCCA CAAACAGGTG GTGGAAAACC AGCAGCTGTT CAGCGCCTTC 
AAGGCCTTGC TGGAGGACTT CCGTGCGGAG CTGCGGGAGG ATGAGCGTGC CCGACTACGG 
CTGCAGCAGC AATATGCCAG CGACAAGGCG GCCTGGGACG TGGAGTGGGC CGTGCTCAAG 
TGCCGTCTGG AACAGAATTG TTGTGGATAT CCCAGAATTA ACATTGAGGA GGAGACTTTA 
GGCTTCACCA GGCTGCCAGC TGGGTCCACG GTAAAAACGT TGAAGAGCCT TGGGTTGCAG 
AGATTGGAGC TGGAAGAGAA GACTGAGAAC AAGTTGGGAG AACTAGGCTC CTCCGCTGAG 
AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG GTGCACCAGA AGCTCCTGGC AGACAGTCAC 
AGCCTGGTCA TGGACCTGCG CTGGCAGATC CATCACAGCG AGAAGAACTG GAACCGGGAG 
AAGGTGGAAC TTCTCGACCG CCTGGACAGA GATCGGCAGG AGTGGGAGCG GCAGAAGAAG 
GAATTCTTGT GGAGGATAGA GCAGTTGCAG AAAGAGAACA GTCCCCGGAG AGGTGGCAGT 
TTCCTCTGTG ATCAAAAAGA CGGCAACGTT CGCCCCTTTC CCCACCAGGG AAGCCTCCGC 
ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT GCAGATGCTG ACTCCATCCC GTTTGAAGAC 
CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC AGGTGCTCGG CCAGTGAGAA TCTCTACCTG 
GATGCCTTGT CCCTGGATGA CGAGCCAGAA GAGCCACCAG CCCACAGGCC CGAGAGGGAG 
TTCAGGAACC GCCTCCCTGA GGAAGAAGAA AATCACAAAG GAAATCTTCA AAGGGCGGTG 
TCCGTGTCCT CCATGTCTGA GTTCCAGCGT CTAATGGACA TCTCCCCCTT CCTGCCTGAG 
AAGGGCCTGC CGTCCACCAG CAGCAAGGAG GATGTCACCC CACCCCTGTC TCCAGACGAC 
CTCAAGTACA TCGAGGAGTT CAACAAGAGC TGGGACTACA CACCCAACAG GGGCCACAAT 
GGTGGGGGGC CGGACCTTTG GGCCGACAGG ACCGAGGTGG GGCGGGCAGG GCACGAGGAC 
AGCACAGAGC CTTTCCCCGA CTCCTCCTGG TACCTAACCA CAAGTGTCAC CATGACCACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCAC TGCGGAGCCA CGTCCTCACC 
GAGCAGTCGG GGTTGCGCGT GTTACACAGC CCGCCTGCCG TGCGCAGGGT CGACAGCATC 
ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC 
ACCAAGGGAG GCCCTCCAGA ACCCATGCTC AGCAGGTGGC CTTGCACCTC CCCCAGGCAC 
TCCCGGGACT ATGTGGAGGG GGCACGGCGC CCCCTTGATA GTCCCCTCTG TACCTCCCTG 
GGGTTTGCCT CCCCACTGCA CAGCCTGGAG ATGTCCAAGA ACTTGAGTGA TGACATGAAG 
GAGGTGGCCT TCTCTGTCAG GAATGCCATC TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACGATGGGGA CCCAGACTGT TCAGACCATC 
AGTGTGGGCT TGCAGACTGA AGCCCTGCGT GGCAGCGGTG TCACCAGCAG CCCCCACAAG 
TGTCTCACTC CAAAGGCTGG GGGCGGTGCT ACACCCGTGT CGTCTCCTTC CCGGAGCCTT 
AGGAGCAGAC AGGTGGCCCC TGCCATCGAG AAGGTGCAGG CCAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTATGGTTC TCCCAAGCTG CAGAGGAAGC CCCTCCCCAA AGCCGACCAG 
CCAAATAACA GGACGTCACC AGGGATGGCC CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC 
CGCTCCACCA CCACAAGGGA GAGCCCCGTG CACACCACCA TTAATGATGG CCTCTCCAGC 
CTCTTCAACA TCATTGACCA CAGCCCCGTG GTGCAGGACC CCTTCCAGAA GGGGCTGCGG 
GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC CGACCAGAGC TGGGCCCAGG CCAGGAAACA 
GGCACCAATT CCCGAGGAAG GTCGCCTAGC CCCATTGGGG TGGGGTCAGA GATGTGCAGG 
GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG CAGGACTTAT CTGCTCCCCC TGGCTACACG 
CTCACTGAGA ACGTGGCCCG GATCCTCAAC AAGAAGCTGC TGGAACATGC CTTAAAGGAG 
GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG GGTCTCCACA GTGACAGCCA CTCGCTGGGG 
GACACAGCCG AGCCAGGGCC CATGGAGGAA CTACCTTGTT CTGCACTAGC TCCATCCCTA 
GAGCCCTGCT TCTCCAGGCC CGAGAGACCA GCAAACCGTC GCCCTCCGTC CCGTTGGGCC 
CCACATTCCC CCACTGCCTC ACAGCCTCAG TCACCCGGAG ACCCGACGTC CTTGGAGGAG 
CATGGTGGCG AGGAGCCGCC CGAGGAGCAG CCACACCGAG ATGCAAGCTT GCATGGATTA 
TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881160.1
CDS	381..5681
Translation	MPLEGPRLRTRLPLLLQLRKELGALRVSWAPTSRVEGAAPFSDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025671.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025671.2

ORF Insert Sequence:

ATGCCACTGG AGGGCCCCCG GCTACGGACC CGCTTGCCCC TTCTCCTGCA ATTACGGAAA 
GAGCTGGGGG CTCTCAGGGT CTCCTGGGCT CCGACATCCC GGGTAGAGGG GGCCGCGCCG 
TTTTCGGATG AGTTAGATGA ACTCCGTGCT GAGATGGAAG AGATGAGAGA CAGTTATTTA 
GAGGAAGATG TTTACCAGCT GCAGGAACTT CGGCGAGAAC TGGACCGCGC TAATAAAAAC 
TGCCGAATCC TGCAGTACCG TCTTCGGAAA GCCGAGCAGA AAAGCCTGAA AGTGGCTGAG 
ACGGGTCAGG TGGATGGTGA GCTTATTCGA AGCCTGGAGC AGGACTTGAA GGTAGCCAAA 
GATGTATCTG TCAGATTGCA CCACGAACTT AAGACGGTGG AGGAAAAGCG CGCTAAAGCT 
GAGGATGAAA ACGAAACTCT CCGACAGCAG ATGATTGAAG TGGAAATATC CAAACAGGCC 
CTCCAGAATG AGCTGGAGAG ACTGAAAGAG AGTTCCCTAA AAAGAAGAAG CACTCGGGAA 
ATGTACAAGG AGAAGAAAAC CTTTAACCAG CAGAATGGTG GAATGGAGCG CCCTGGGAAC 
TGCAGGCCCG CAACCAAAAC CCAAAGGAAG CTGGCGCCCC GGCGCAAGGA TGACAGTGCC 
GATTTGAGGT GCCAGCTCCA GTTTGCCAAA GAGGAAGCCT TCCTGATGCG CAAAAAGATG 
GCCAAGCTAG GAAGGGAGAA GGACGAGCTG GAGCAGGAGC TCCAGAAGTA CAAGTCCCTC 
TATGGGGATG TGGACAGTCC CCTGCCCACG GGGGAAGCAG GCGGGCCCCC CAGCACCCGG 
GAGGCCGAGC TGAAGCTGCG GCTAAAGCTG GTGGAGGAGG AAGCCAACAT CTTGGGCCGG 
AAGATCGTGG AGCTGGAGGT GGAGAACCGT GGCCTCAAGG CAGAGATGGA GGACATGCGG 
GGCCAGCAGG AGCGGGAGGG CCCGGGTCGG GACCACGCAC CCAGCATTCC TACCTCACCC 
TTCGGTGACT CCCTGGAGTC CTCCACTGAG CTCCGCCGCC ACCTGCAGTT TGTAGAAGAG 
GAAGCGGAGT TGCTCCGGAG GTCCATCTCC GAGATCGAAG ACCACAACCG GCAACTGACC 
CACGAGCTCA GCAAGTTTAA GTTTGAGCCT CCCCGGGAGC CGGGCTGGCT AGGAGAGGGT 
GCAAGTCCTG GTGCCGGGGG TGGGGCCCCC CTGCAGGAGG AGCTGAAGTC AGCCAGGCTG 
CAGATCAGCG AGCTCAGCGG CAAGGTGCTC AAACTGCAGC ACGAGAACCA CGCGCTGCTG 
TCCAACATCC AGCGCTGCGA CCTGGCAGCC CACCTGGGGC TGCGTGCCCC CAGTCCCCGG 
GACAGCGATG CCGAGAGTGA TGCGGGCAAG AAGGAGAGTG ATGGGGAGGA GAGCCGCCTG 
CCCCAGCCCA AGCGGGAAGG GCCTGTTGGC GGGGAGAGTG ACTCGGAGGA AATGTTTGAG 
AAGACGTCGG GCTTCGGGAG CGGGAAGCCA TCGGAGGCCA GCGAGCCATG CCCCACGGAG 
CTCCTGAAGG CCCGGGAGGA CTCTGAGTAC CTAGTGACCC TAAAACACGA GGCCCAGCGG 
CTAGAGCGGA CGGTGGAGCG CCTCATCACG GACACCGACA GCTTCCTCCA TGATGCGGGG 
CTGCGGGGTG GTGCGCCCTT ACCGGGGCCT GGCCTCCAGG GCGAAGAGGA GCAGGGTGAG 
GGGGACCAGC AGGAGCCCCA GCTGCTGGGG ACCATCAACG CCAAGATGAA GGCTTTCAAG 
AAAGAGCTGC AGGCCTTCCT GGAGCAGGTG AACCGCATTG GGGATGGCCT ATCCCCCTTG 
CCCCACCTCA CAGAGTCCTC TAGCTTCCTC TCCACTGTGA CTTCCGTGTC CCGGGACTCC 
CCCATCGGGA ACCTGGGGAA GGAGCTGGGC CCAGACTTGC AGTCCAGACT GAAAGAGCAG 
CTGGAGTGGC AGCTCGGGCC GGCCCGAGGG GACGAGCGGG AGAGCCTGCG CCTCCGAGCC 
GCGCGGGAGC TGCACCGCCG CGCAGACGGG GACACCGGGA GCCACGGGCT GGGAGGCCAG 
ACCTGCTTCA GCCTGGAGCT CAGGGGCCCC CCCGTTTTAC CTGAGCAGAG TGTATCCATA 
GAGGAGCTAC AGGGTCAGCT CGTGCAGGCG GCCAGACTGC ATCAAGAGGA GACAGAGACA 
TTTACAAACA AGATCCATAA GATGGAGGAG GAGCACCTCT ATGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAAAAC ACCCTCCATG AGCGAACCTG GAGTGATGAG 
AAGAATCTGA TGCAGCAGGA GCTCCGGTCC TTGAAGCAGA ACATTTTCCT CTTCTACGTC 
AAACTCAGGT GGCTGCTGAA ACACTGGCGG CAAGGGAAGC AGATGGAGGA GGAAGGAGAG 
GAGTTCACTG AGGGTGAACA TCCAGAGACC CTCTCCAGGC TCGGGGAGCT TGGAGTCCAG 
GGGGGTCACC AGGCGGATGG CCCAGACCAC GACAGTGACC GAGGCTGTGG CTTTCCAGTG 
GGGGAGCACT CCCCACACTC CCGGGTGCAG ATTGGAGATC ACAGCTTGCG GCTGCAGACC 
GCGGACAGGG GACAGCCCCA CAAACAGGTG GTGGAAAACC AGCAGCTGTT CAGCGCCTTC 
AAGGCCTTGC TGGAGGACTT CCGTGCGGAG CTGCGGGAGG ATGAGCGTGC CCGACTACGG 
CTGCAGCAGC AATATGCCAG CGACAAGGCG GCCTGGGACG TGGAGTGGGC CGTGCTCAAG 
TGCCGTCTGG AACAGAATTG TTGTGGATAT CCCAGAATTA ACATTGAGGA GGAGACTTTA 
GGCTTCACCA GGCTGCCAGC TGGGTCCACG GTAAAAACGT TGAAGAGCCT TGGGTTGCAG 
AGATTGGAGC TGGAAGAGAA GACTGAGAAC AAGTTGGGAG AACTAGGCTC CTCCGCTGAG 
AGCAAGGGGG CCTTGAAGAA GGAGAGAGAG GTGCACCAGA AGCTCCTGGC AGACAGTCAC 
AGCCTGGTCA TGGACCTGCG CTGGCAGATC CATCACAGCG AGAAGAACTG GAACCGGGAG 
AAGGTGGAAC TTCTCGACCG CCTGGACAGA GATCGGCAGG AGTGGGAGCG GCAGAAGAAG 
GAATTCTTGT GGAGGATAGA GCAGTTGCAG AAAGAGAACA GTCCCCGGAG AGGTGGCAGT 
TTCCTCTGTG ATCAAAAAGA CGGCAACGTT CGCCCCTTTC CCCACCAGGG AAGCCTCCGC 
ATGCCCCGTC CAGTGGCCAT GTGGCCTTGT GCAGATGCTG ACTCCATCCC GTTTGAAGAC 
CGGCCGCTGT CCAAGCTGAA GGAGTCGGAC AGGTGCTCGG CCAGTGAGAA TCTCTACCTG 
GATGCCTTGT CCCTGGATGA CGAGCCAGAA GAGCCACCAG CCCACAGGCC CGAGAGGGAG 
TTCAGGAACC GCCTCCCTGA GGAAGAAGAA AATCACAAAG GAAATCTTCA AAGGGCGGTG 
TCCGTGTCCT CCATGTCTGA GTTCCAGCGT CTAATGGACA TCTCCCCCTT CCTGCCTGAG 
AAGGGCCTGC CGTCCACCAG CAGCAAGGAG GATGTCACCC CACCCCTGTC TCCAGACGAC 
CTCAAGTACA TCGAGGAGTT CAACAAGAGC TGGGACTACA CACCCAACAG GGGCCACAAT 
GGTGGGGGGC CGGACCTTTG GGCCGACAGG ACCGAGGTGG GGCGGGCAGG GCACGAGGAC 
AGCACAGAGC CTTTCCCCGA CTCCTCCTGG TACCTAACCA CAAGTGTCAC CATGACCACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCAC TGCGGAGCCA CGTCCTCACC 
GAGCAGTCGG GGTTGCGCGT GTTACACAGC CCGCCTGCCG TGCGCAGGGT CGACAGCATC 
ACGGCGGCAG GTGGTGAGGG TCCCTTTCCC ACAAGCAGAG CCAGAGGGAG CCCGGGAGAC 
ACCAAGGGAG GCCCTCCAGA ACCCATGCTC AGCAGGTGGC CTTGCACCTC CCCCAGGCAC 
TCCCGGGACT ATGTGGAGGG GGCACGGCGC CCCCTTGATA GTCCCCTCTG TACCTCCCTG 
GGGTTTGCCT CCCCACTGCA CAGCCTGGAG ATGTCCAAGA ACTTGAGTGA TGACATGAAG 
GAGGTGGCCT TCTCTGTCAG GAATGCCATC TGCTCCGGCC CTGGCGAGCT GCAAGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACGATGGGGA CCCAGACTGT TCAGACCATC 
AGTGTGGGCT TGCAGACTGA AGCCCTGCGT GGCAGCGGTG TCACCAGCAG CCCCCACAAG 
TGTCTCACTC CAAAGGCTGG GGGCGGTGCT ACACCCGTGT CGTCTCCTTC CCGGAGCCTT 
AGGAGCAGAC AGGTGGCCCC TGCCATCGAG AAGGTGCAGG CCAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTATGGTTC TCCCAAGCTG CAGAGGAAGC CCCTCCCCAA AGCCGACCAG 
CCAAATAACA GGACGTCACC AGGGATGGCC CAGAAAGGGT ACAGTGAGTC AGCCTGGGCC 
CGCTCCACCA CCACAAGGGA GAGCCCCGTG CACACCACCA TTAATGATGG CCTCTCCAGC 
CTCTTCAACA TCATTGACCA CAGCCCCGTG GTGCAGGACC CCTTCCAGAA GGGGCTGCGG 
GCCGGCAGTC GGTCTCGCTC AGCAGAGCCC CGACCAGAGC TGGGCCCAGG CCAGGAAACA 
GGCACCAATT CCCGAGGAAG GTCGCCTAGC CCCATTGGGG TGGGGTCAGA GATGTGCAGG 
GAGGAAGGGG GAGAGGGCAC GCCAGTGAAG CAGGACTTAT CTGCTCCCCC TGGCTACACG 
CTCACTGAGA ACGTGGCCCG GATCCTCAAC AAGAAGCTGC TGGAACATGC CTTAAAGGAG 
GAGAGGAGGC AGGCTGCCCA CGGGCCCCCG GGTCTCCACA GTGACAGCCA CTCGCTGGGG 
GACACAGCCG AGCCAGGGCC CATGGAGGAA CTACCTTGTT CTGCACTAGC TCCATCCCTA 
GAGCCCTGCT TCTCCAGGCC CGAGAGACCA GCAAACCGTC GCCCTCCGTC CCGTTGGGCC 
CCACATTCCC CCACTGCCTC ACAGCCTCAG TCACCCGGAG ACCCGACGTC CTTGGAGGAG 
CATGGTGGCG AGGAGCCGCC CGAGGAGCAG CCACACCGAG ATGCAAGCTT GCATGGATTA 
TCACAGTATA ATTCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025672.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881161.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X18, mRNA.

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CloneID	OHu87829
Clone ID Related Accession (Same CDS sequence)	XM_017025672.1 , XM_024451122.1 , XM_017025674.2 , XM_017025674.1 , XM_017025673.1
Accession Version	XM_017025672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881161.1
CDS	136..5283
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025672.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025672.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024451122.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306890.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X20, mRNA.

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CloneID	OHu87829
Clone ID Related Accession (Same CDS sequence)	XM_017025672.1 , XM_024451122.1 , XM_017025674.2 , XM_017025674.1 , XM_017025673.1
Accession Version	XM_024451122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306890.1
CDS	79..5226
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_024451122.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451122.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025674.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881163.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X21, mRNA.

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CloneID	OHu87829
Clone ID Related Accession (Same CDS sequence)	XM_017025672.1 , XM_024451122.1 , XM_017025674.2 , XM_017025674.1 , XM_017025673.1
Accession Version	XM_017025674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881163.1
CDS	893..6040
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025674.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025674.2

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025674.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881163.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu87829
Clone ID Related Accession (Same CDS sequence)	XM_017025672.1 , XM_024451122.1 , XM_017025674.2 , XM_017025674.1 , XM_017025673.1
Accession Version	XM_017025674.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881163.1
CDS	190..5337
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025674.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025674.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025673.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881162.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu87829
Clone ID Related Accession (Same CDS sequence)	XM_017025672.1 , XM_024451122.1 , XM_017025674.2 , XM_017025674.1 , XM_017025673.1
Accession Version	XM_017025673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881162.1
CDS	143..5290
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025673.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025673.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GTTGCAGAAA 
GAGAACAGTC CCCGGAGAGG TGGCAGTTTC CTCTGTGATC AAAAAGACGG CAACGTTCGC 
CCCTTTCCCC ACCAGGGAAG CCTCCGCATG CCCCGTCCAG TGGCCATGTG GCCTTGTGCA 
GATGCTGACT CCATCCCGTT TGAAGACCGG CCGCTGTCCA AGCTGAAGGA GTCGGACAGG 
TGCTCGGCCA GTGAGAATCT CTACCTGGAT GCCTTGTCCC TGGATGACGA GCCAGAAGAG 
CCACCAGCCC ACAGGCCCGA GAGGGAGTTC AGGAACCGCC TCCCTGAGGA AGAAGAAAAT 
CACAAAGGAA ATCTTCAAAG GGCGGTGTCC GTGTCCTCCA TGTCTGAGTT CCAGCGTCTA 
ATGGACATCT CCCCCTTCCT GCCTGAGAAG GGCCTGCCGT CCACCAGCAG CAAGGAGGAT 
GTCACCCCAC CCCTGTCTCC AGACGACCTC AAGTACATCG AGGAGTTCAA CAAGAGCTGG 
GACTACACAC CCAACAGGGG CCACAATGGT GGGGGGCCGG ACCTTTGGGC CGACAGGACC 
GAGGTGGGGC GGGCAGGGCA CGAGGACAGC ACAGAGCCTT TCCCCGACTC CTCCTGGTAC 
CTAACCACAA GTGTCACCAT GACCACGGAC ACCATGACCA GCCCAGAGCA CTGCCAGAAG 
CAGCCACTGC GGAGCCACGT CCTCACCGAG CAGTCGGGGT TGCGCGTGTT ACACAGCCCG 
CCTGCCGTGC GCAGGGTCGA CAGCATCACG GCGGCAGGTG GTGAGGGTCC CTTTCCCACA 
AGCAGAGCCA GAGGGAGCCC GGGAGACACC AAGGGAGGCC CTCCAGAACC CATGCTCAGC 
AGGTGGCCTT GCACCTCCCC CAGGCACTCC CGGGACTATG TGGAGGGGGC ACGGCGCCCC 
CTTGATAGTC CCCTCTGTAC CTCCCTGGGG TTTGCCTCCC CACTGCACAG CCTGGAGATG 
TCCAAGAACT TGAGTGATGA CATGAAGGAG GTGGCCTTCT CTGTCAGGAA TGCCATCTGC 
TCCGGCCCTG GCGAGCTGCA AGTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ATGGGGACCC AGACTGTTCA GACCATCAGT GTGGGCTTGC AGACTGAAGC CCTGCGTGGC 
AGCGGTGTCA CCAGCAGCCC CCACAAGTGT CTCACTCCAA AGGCTGGGGG CGGTGCTACA 
CCCGTGTCGT CTCCTTCCCG GAGCCTTAGG AGCAGACAGG TGGCCCCTGC CATCGAGAAG 
GTGCAGGCCA AGTTTGAACG CACATGCTGC TCCCCCAAGT ATGGTTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC CGACCAGCCA AATAACAGGA CGTCACCAGG GATGGCCCAG 
AAAGGGTACA GTGAGTCAGC CTGGGCCCGC TCCACCACCA CAAGGGAGAG CCCCGTGCAC 
ACCACCATTA ATGATGGCCT CTCCAGCCTC TTCAACATCA TTGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GCTGCGGGCC GGCAGTCGGT CTCGCTCAGC AGAGCCCCGA 
CCAGAGCTGG GCCCAGGCCA GGAAACAGGC ACCAATTCCC GAGGAAGGTC GCCTAGCCCC 
ATTGGGGTGG GGTCAGAGAT GTGCAGGGAG GAAGGGGGAG AGGGCACGCC AGTGAAGCAG 
GACTTATCTG CTCCCCCTGG CTACACGCTC ACTGAGAACG TGGCCCGGAT CCTCAACAAG 
AAGCTGCTGG AACATGCCTT AAAGGAGGAG AGGAGGCAGG CTGCCCACGG GCCCCCGGGT 
CTCCACAGTG ACAGCCACTC GCTGGGGGAC ACAGCCGAGC CAGGGCCCAT GGAGGAACTA 
CCTTGTTCTG CACTAGCTCC ATCCCTAGAG CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC CTCCGTCCCG TTGGGCCCCA CATTCCCCCA CTGCCTCACA GCCTCAGTCA 
CCCGGAGACC CGACGTCCTT GGAGGAGCAT GGTGGCGAGG AGCCGCCCGA GGAGCAGCCA 
CACCGAGATG CAAGCTTGCA TGGATTATCA CAGTATAATT CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_017025675.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881164.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X22, mRNA.

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CloneID	OHu87830
Clone ID Related Accession (Same CDS sequence)	XM_017025675.1
Accession Version	XM_017025675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGAATTGT TGTGGATATC CCAGAATTAA CATTGAGGAG 
GAGACTTTAG GCTTCACCAG GCTGCCAGCT GGGTCCACGG TAAAAACGTT GAAGAGCCTT 
GGGTTGCAGA GATTGGAGCT GGAAGAGAAG ACTGAGAACA AGTTGGGAGA ACTAGGCTCC 
TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG GAGAGAGAGG TGCACCAGAA GCTCCTGGCA 
GACAGTCACA GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGCGA GAAGAACTGG 
AACCGGGAGA AGGTGGAACT TCTCGACCGC CTGGACAGAG ATCGGCAGGA GTGGGAGCGG 
CAGAAGAAGG AATTCTTGTG GAGGATAGAG CAGTTGCAGA AAGAGAACAG TCCCCGGAGA 
GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC GGCAACGTTC GCCCCTTTCC CCACCAGGGA 
AGCCTCCGCA TGCCCCGTCC AGTGGCCATG TGGCCTTGTG CAGATGCTGA CTCCATCCCG 
TTTGAAGACC GGCCGCTGTC CAAGCTGAAG GAGTCGGACA GGTGCTCGGC CAGTGAGAAT 
CTCTACCTGG ATGCCTTGTC CCTGGATGAC GAGCCAGAAG AGCCACCAGC CCACAGGCCC 
GAGAGGGAGT TCAGGAACCG CCTCCCTGAG GAAGAAGAAA ATCACAAAGG AAATCTTCAA 
AGGGCGGTGT CCGTGTCCTC CATGTCTGAG TTCCAGCGTC TAATGGACAT CTCCCCCTTC 
CTGCCTGAGA AGGGCCTGCC GTCCACCAGC AGCAAGGAGG ATGTCACCCC ACCCCTGTCT 
CCAGACGACC TCAAGTACAT CGAGGAGTTC AACAAGAGCT GGGACTACAC ACCCAACAGG 
GGCCACAATG GTGGGGGGCC GGACCTTTGG GCCGACAGGA CCGAGGTGGG GCGGGCAGGG 
CACGAGGACA GCACAGAGCC TTTCCCCGAC TCCTCCTGGT ACCTAACCAC AAGTGTCACC 
ATGACCACGG ACACCATGAC CAGCCCAGAG CACTGCCAGA AGCAGCCACT GCGGAGCCAC 
GTCCTCACCG AGCAGTCGGG GTTGCGCGTG TTACACAGCC CGCCTGCCGT GCGCAGGGTC 
GACAGCATCA CGGCGGCAGG TGGTGAGGGT CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC 
CCGGGAGACA CCAAGGGAGG CCCTCCAGAA CCCATGCTCA GCAGGTGGCC TTGCACCTCC 
CCCAGGCACT CCCGGGACTA TGTGGAGGGG GCACGGCGCC CCCTTGATAG TCCCCTCTGT 
ACCTCCCTGG GGTTTGCCTC CCCACTGCAC AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCTGTCAGG AATGCCATCT GCTCCGGCCC TGGCGAGCTG 
CAAGTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGATGGGGAC CCAGACTGTT 
CAGACCATCA GTGTGGGCTT GCAGACTGAA GCCCTGCGTG GCAGCGGTGT CACCAGCAGC 
CCCCACAAGT GTCTCACTCC AAAGGCTGGG GGCGGTGCTA CACCCGTGTC GTCTCCTTCC 
CGGAGCCTTA GGAGCAGACA GGTGGCCCCT GCCATCGAGA AGGTGCAGGC CAAGTTTGAA 
CGCACATGCT GCTCCCCCAA GTATGGTTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCCGACCAGC CAAATAACAG GACGTCACCA GGGATGGCCC AGAAAGGGTA CAGTGAGTCA 
GCCTGGGCCC GCTCCACCAC CACAAGGGAG AGCCCCGTGC ACACCACCAT TAATGATGGC 
CTCTCCAGCC TCTTCAACAT CATTGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA GCAGAGCCCC GACCAGAGCT GGGCCCAGGC 
CAGGAAACAG GCACCAATTC CCGAGGAAGG TCGCCTAGCC CCATTGGGGT GGGGTCAGAG 
ATGTGCAGGG AGGAAGGGGG AGAGGGCACG CCAGTGAAGC AGGACTTATC TGCTCCCCCT 
GGCTACACGC TCACTGAGAA CGTGGCCCGG ATCCTCAACA AGAAGCTGCT GGAACATGCC 
TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC GGGCCCCCGG GTCTCCACAG TGACAGCCAC 
TCGCTGGGGG ACACAGCCGA GCCAGGGCCC ATGGAGGAAC TACCTTGTTC TGCACTAGCT 
CCATCCCTAG AGCCCTGCTT CTCCAGGCCC GAGAGACCAG CAAACCGTCG CCCTCCGTCC 
CGTTGGGCCC CACATTCCCC CACTGCCTCA CAGCCTCAGT CACCCGGAGA CCCGACGTCC 
TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC GAGGAGCAGC CACACCGAGA TGCAAGCTTG 
CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881164.1
CDS	143..5212
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_017025675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025675.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGAATTGT TGTGGATATC CCAGAATTAA CATTGAGGAG 
GAGACTTTAG GCTTCACCAG GCTGCCAGCT GGGTCCACGG TAAAAACGTT GAAGAGCCTT 
GGGTTGCAGA GATTGGAGCT GGAAGAGAAG ACTGAGAACA AGTTGGGAGA ACTAGGCTCC 
TCCGCTGAGA GCAAGGGGGC CTTGAAGAAG GAGAGAGAGG TGCACCAGAA GCTCCTGGCA 
GACAGTCACA GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGCGA GAAGAACTGG 
AACCGGGAGA AGGTGGAACT TCTCGACCGC CTGGACAGAG ATCGGCAGGA GTGGGAGCGG 
CAGAAGAAGG AATTCTTGTG GAGGATAGAG CAGTTGCAGA AAGAGAACAG TCCCCGGAGA 
GGTGGCAGTT TCCTCTGTGA TCAAAAAGAC GGCAACGTTC GCCCCTTTCC CCACCAGGGA 
AGCCTCCGCA TGCCCCGTCC AGTGGCCATG TGGCCTTGTG CAGATGCTGA CTCCATCCCG 
TTTGAAGACC GGCCGCTGTC CAAGCTGAAG GAGTCGGACA GGTGCTCGGC CAGTGAGAAT 
CTCTACCTGG ATGCCTTGTC CCTGGATGAC GAGCCAGAAG AGCCACCAGC CCACAGGCCC 
GAGAGGGAGT TCAGGAACCG CCTCCCTGAG GAAGAAGAAA ATCACAAAGG AAATCTTCAA 
AGGGCGGTGT CCGTGTCCTC CATGTCTGAG TTCCAGCGTC TAATGGACAT CTCCCCCTTC 
CTGCCTGAGA AGGGCCTGCC GTCCACCAGC AGCAAGGAGG ATGTCACCCC ACCCCTGTCT 
CCAGACGACC TCAAGTACAT CGAGGAGTTC AACAAGAGCT GGGACTACAC ACCCAACAGG 
GGCCACAATG GTGGGGGGCC GGACCTTTGG GCCGACAGGA CCGAGGTGGG GCGGGCAGGG 
CACGAGGACA GCACAGAGCC TTTCCCCGAC TCCTCCTGGT ACCTAACCAC AAGTGTCACC 
ATGACCACGG ACACCATGAC CAGCCCAGAG CACTGCCAGA AGCAGCCACT GCGGAGCCAC 
GTCCTCACCG AGCAGTCGGG GTTGCGCGTG TTACACAGCC CGCCTGCCGT GCGCAGGGTC 
GACAGCATCA CGGCGGCAGG TGGTGAGGGT CCCTTTCCCA CAAGCAGAGC CAGAGGGAGC 
CCGGGAGACA CCAAGGGAGG CCCTCCAGAA CCCATGCTCA GCAGGTGGCC TTGCACCTCC 
CCCAGGCACT CCCGGGACTA TGTGGAGGGG GCACGGCGCC CCCTTGATAG TCCCCTCTGT 
ACCTCCCTGG GGTTTGCCTC CCCACTGCAC AGCCTGGAGA TGTCCAAGAA CTTGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCTGTCAGG AATGCCATCT GCTCCGGCCC TGGCGAGCTG 
CAAGTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGATGGGGAC CCAGACTGTT 
CAGACCATCA GTGTGGGCTT GCAGACTGAA GCCCTGCGTG GCAGCGGTGT CACCAGCAGC 
CCCCACAAGT GTCTCACTCC AAAGGCTGGG GGCGGTGCTA CACCCGTGTC GTCTCCTTCC 
CGGAGCCTTA GGAGCAGACA GGTGGCCCCT GCCATCGAGA AGGTGCAGGC CAAGTTTGAA 
CGCACATGCT GCTCCCCCAA GTATGGTTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCCGACCAGC CAAATAACAG GACGTCACCA GGGATGGCCC AGAAAGGGTA CAGTGAGTCA 
GCCTGGGCCC GCTCCACCAC CACAAGGGAG AGCCCCGTGC ACACCACCAT TAATGATGGC 
CTCTCCAGCC TCTTCAACAT CATTGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGCTGCGGG CCGGCAGTCG GTCTCGCTCA GCAGAGCCCC GACCAGAGCT GGGCCCAGGC 
CAGGAAACAG GCACCAATTC CCGAGGAAGG TCGCCTAGCC CCATTGGGGT GGGGTCAGAG 
ATGTGCAGGG AGGAAGGGGG AGAGGGCACG CCAGTGAAGC AGGACTTATC TGCTCCCCCT 
GGCTACACGC TCACTGAGAA CGTGGCCCGG ATCCTCAACA AGAAGCTGCT GGAACATGCC 
TTAAAGGAGG AGAGGAGGCA GGCTGCCCAC GGGCCCCCGG GTCTCCACAG TGACAGCCAC 
TCGCTGGGGG ACACAGCCGA GCCAGGGCCC ATGGAGGAAC TACCTTGTTC TGCACTAGCT 
CCATCCCTAG AGCCCTGCTT CTCCAGGCCC GAGAGACCAG CAAACCGTCG CCCTCCGTCC 
CGTTGGGCCC CACATTCCCC CACTGCCTCA CAGCCTCAGT CACCCGGAGA CCCGACGTCC 
TTGGAGGAGC ATGGTGGCGA GGAGCCGCCC GAGGAGCAGC CACACCGAGA TGCAAGCTTG 
CATGGATTAT CACAGTATAA TTCACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( NM_015210.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056025.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.

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CloneID	OHu27694
Clone ID Related Accession (Same CDS sequence)	NM_015210.4 , NM_015210.3
Accession Version	NM_015210.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131528.1, AP000864.4, AB018345.2 and AP001531.4. On Jun 2, 2019 this sequence version replaced NM_015210.3. ##Evidence-Data-START## Transcript exon combination :: AK131528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056025.2
CDS	133..4893
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	NM_015210.4
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015210.4

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( NM_015210.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056025.2 Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.

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CloneID	OHu27694
Clone ID Related Accession (Same CDS sequence)	NM_015210.4 , NM_015210.3
Accession Version	NM_015210.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131528.1, AP000864.4 and AB018345.2. On Dec 20, 2007 this sequence version replaced NM_015210.2. ##Evidence-Data-START## Transcript exon combination :: AK131528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056025.2
CDS	143..4903
Translation	MEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	NM_015210.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015210.3

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005258098.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258155.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

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CloneID	OHu50999
Clone ID Related Accession (Same CDS sequence)	XM_005258098.3
Accession Version	XM_005258098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258155.1
CDS	86..5998
Translation	METLNGPAGGGAPDAKLQPPGQHHRHHHLHPVAERRRLHRAPSPARPFLKDLHARPAAPGPAVPSSGRAPAPAAPRSPNLAGKAPPSPGSLAAPGRLSRRSGGVPGAKDKPPPGAGARAAGGAKAALGSRRAARVAPAEPLSRAGKPPGAEPPSAAAKGRKAKRGSRAPPARTVGPPTPAARIPAVTLAVTSVAGSPARCSRISHTDSSSDLSDCPSEPLSDEQRLLPAASSDAESGTGSSDREPPRGAPTPSPAARGAPPGSPEPPALLAAPLAAGACPGGRSIPSGVSGGFAGPGVAEDVRGRSPPERPVPGTPKEPSLGEQSRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_005258098.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258098.3

ORF Insert Sequence:

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGG AACTACCTTG TTCTGCACTA 
GCTCCATCCC TAGAGCCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCCTCCG 
TCCCGTTGGG CCCCACATTC CCCCACTGCC TCACAGCCTC AGTCACCCGG AGACCCGACG 
TCCTTGGAGG AGCATGGTGG CGAGGAGCCG CCCGAGGAGC AGCCACACCG AGATGCAAGC 
TTGCATGGAT TATCACAGTA TAATTCACTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005258099.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258156.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

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CloneID	OHu51000
Clone ID Related Accession (Same CDS sequence)	XM_005258099.4
Accession Version	XM_005258099.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258099.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGA ACCAAACTGT CTTGCTAACT 
GCCCCCTGGG GACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258156.1
CDS	86..5803
Translation	METLNGPAGGGAPDAKLQPPGQHHRHHHLHPVAERRRLHRAPSPARPFLKDLHARPAAPGPAVPSSGRAPAPAAPRSPNLAGKAPPSPGSLAAPGRLSRRSGGVPGAKDKPPPGAGARAAGGAKAALGSRRAARVAPAEPLSRAGKPPGAEPPSAAAKGRKAKRGSRAPPARTVGPPTPAARIPAVTLAVTSVAGSPARCSRISHTDSSSDLSDCPSEPLSDEQRLLPAASSDAESGTGSSDREPPRGAPTPSPAARGAPPGSPEPPALLAAPLAAGACPGGRSIPSGVSGGFAGPGVAEDVRGRSPPERPVPGTPKEPSLGEQSRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLEMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_005258099.4
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258099.4

ORF Insert Sequence:

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGATG GAGGAGGAGC ACCTCTATGC CTTGAGGTGG 
AAAGAACTGG AAATGCACAG CCTGGCTTTG CAAAACACCC TCCATGAGCG AACCTGGAGT 
GATGAGAAGA ATCTGATGCA GCAGGAGCTC CGGTCCTTGA AGCAGAACAT TTTCCTCTTC 
TACGTCAAAC TCAGGTGGCT GCTGAAACAC TGGCGGCAAG GGAAGCAGAT GGAGGAGGAA 
GGAGAGGAGT TCACTGAGGG TGAACATCCA GAGACCCTCT CCAGGCTCGG GGAGCTTGGA 
GTCCAGGGGG GTCACCAGGC GGATGGCCCA GACCACGACA GTGACCGAGG CTGTGGCTTT 
CCAGTGGGGG AGCACTCCCC ACACTCCCGG GTGCAGATTG GAGATCACAG CTTGCGGCTG 
CAGACCGCGG ACAGGGGACA GCCCCACAAA CAGGTGGTGG AAAACCAGCA GCTGTTCAGC 
GCCTTCAAGG CCTTGCTGGA GGACTTCCGT GCGGAGCTGC GGGAGGATGA GCGTGCCCGA 
CTACGGCTGC AGCAGCAATA TGCCAGCGAC AAGGCGGCCT GGGACGTGGA GTGGGCCGTG 
CTCAAGTGCC GTCTGGAACA GCTGGAAGAG AAGACTGAGA ACAAGTTGGG AGAACTAGGC 
TCCTCCGCTG AGAGCAAGGG GGCCTTGAAG AAGGAGAGAG AGGTGCACCA GAAGCTCCTG 
GCAGACAGTC ACAGCCTGGT CATGGACCTG CGCTGGCAGA TCCATCACAG CGAGAAGAAC 
TGGAACCGGG AGAAGGTGGA ACTTCTCGAC CGCCTGGACA GAGATCGGCA GGAGTGGGAG 
CGGCAGAAGA AGGAATTCTT GTGGAGGATA GAGCAGTTGC AGAAAGAGAA CAGTCCCCGG 
AGAGGTGGCA GTTTCCTCTG TGATCAAAAA GACGGCAACG TTCGCCCCTT TCCCCACCAG 
GGAAGCCTCC GCATGCCCCG TCCAGTGGCC ATGTGGCCTT GTGCAGATGC TGACTCCATC 
CCGTTTGAAG ACCGGCCGCT GTCCAAGCTG AAGGAGTCGG ACAGGTGCTC GGCCAGTGAG 
AATCTCTACC TGGATGCCTT GTCCCTGGAT GACGAGCCAG AAGAGCCACC AGCCCACAGG 
CCCGAGAGGG AGTTCAGGAA CCGCCTCCCT GAGGAAGAAG AAAATCACAA AGGAAATCTT 
CAAAGGGCGG TGTCCGTGTC CTCCATGTCT GAGTTCCAGC GTCTAATGGA CATCTCCCCC 
TTCCTGCCTG AGAAGGGCCT GCCGTCCACC AGCAGCAAGG AGGATGTCAC CCCACCCCTG 
TCTCCAGACG ACCTCAAGTA CATCGAGGAG TTCAACAAGA GCTGGGACTA CACACCCAAC 
AGGGGCCACA ATGGTGGGGG GCCGGACCTT TGGGCCGACA GGACCGAGGT GGGGCGGGCA 
GGGCACGAGG ACAGCACAGA GCCTTTCCCC GACTCCTCCT GGTACCTAAC CACAAGTGTC 
ACCATGACCA CGGACACCAT GACCAGCCCA GAGCACTGCC AGAAGCAGCC ACTGCGGAGC 
CACGTCCTCA CCGAGCAGTC GGGGTTGCGC GTGTTACACA GCCCGCCTGC CGTGCGCAGG 
GTCGACAGCA TCACGGCGGC AGGTGGTGAG GGTCCCTTTC CCACAAGCAG AGCCAGAGGG 
AGCCCGGGAG ACACCAAGGG AGGCCCTCCA GAACCCATGC TCAGCAGGTG GCCTTGCACC 
TCCCCCAGGC ACTCCCGGGA CTATGTGGAG GGGGCACGGC GCCCCCTTGA TAGTCCCCTC 
TGTACCTCCC TGGGGTTTGC CTCCCCACTG CACAGCCTGG AGATGTCCAA GAACTTGAGT 
GATGACATGA AGGAGGTGGC CTTCTCTGTC AGGAATGCCA TCTGCTCCGG CCCTGGCGAG 
CTGCAAGTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGATGGG GACCCAGACT 
GTTCAGACCA TCAGTGTGGG CTTGCAGACT GAAGCCCTGC GTGGCAGCGG TGTCACCAGC 
AGCCCCCACA AGTGTCTCAC TCCAAAGGCT GGGGGCGGTG CTACACCCGT GTCGTCTCCT 
TCCCGGAGCC TTAGGAGCAG ACAGGTGGCC CCTGCCATCG AGAAGGTGCA GGCCAAGTTT 
GAACGCACAT GCTGCTCCCC CAAGTATGGT TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCCGACC AGCCAAATAA CAGGACGTCA CCAGGGATGG CCCAGAAAGG GTACAGTGAG 
TCAGCCTGGG CCCGCTCCAC CACCACAAGG GAGAGCCCCG TGCACACCAC CATTAATGAT 
GGCCTCTCCA GCCTCTTCAA CATCATTGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGCTGC GGGCCGGCAG TCGGTCTCGC TCAGCAGAGC CCCGACCAGA GCTGGGCCCA 
GGCCAGGAAA CAGGCACCAA TTCCCGAGGA AGGTCGCCTA GCCCCATTGG GGTGGGGTCA 
GAGATGTGCA GGGAGGAAGG GGGAGAGGGC ACGCCAGTGA AGCAGGACTT ATCTGCTCCC 
CCTGGCTACA CGCTCACTGA GAACGTGGCC CGGATCCTCA ACAAGAAGCT GCTGGAACAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCTGCC CACGGGCCCC CGGGTCTCCA CAGTGACAGC 
CACTCGCTGG GGGACACAGC CGAGCCAGGG CCCATGGAGA ACCAAACTGT CTTGCTAACT 
GCCCCCTGGG GACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_011525640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523942.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

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CloneID	OHu65813
Clone ID Related Accession (Same CDS sequence)	XM_011525640.2
Accession Version	XM_011525640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GGGAAGCCTC 
CGCATGCCCC GTCCAGTGGC CATGTGGCCT TGTGCAGATG CTGACTCCAT CCCGTTTGAA 
GACCGGCCGC TGTCCAAGCT GAAGGAGTCG GACAGGTGCT CGGCCAGTGA GAATCTCTAC 
CTGGATGCCT TGTCCCTGGA TGACGAGCCA GAAGAGCCAC CAGCCCACAG GCCCGAGAGG 
GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA GAAAATCACA AAGGAAATCT TCAAAGGGCG 
GTGTCCGTGT CCTCCATGTC TGAGTTCCAG CGTCTAATGG ACATCTCCCC CTTCCTGCCT 
GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG GAGGATGTCA CCCCACCCCT GTCTCCAGAC 
GACCTCAAGT ACATCGAGGA GTTCAACAAG AGCTGGGACT ACACACCCAA CAGGGGCCAC 
AATGGTGGGG GGCCGGACCT TTGGGCCGAC AGGACCGAGG TGGGGCGGGC AGGGCACGAG 
GACAGCACAG AGCCTTTCCC CGACTCCTCC TGGTACCTAA CCACAAGTGT CACCATGACC 
ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CACTGCGGAG CCACGTCCTC 
ACCGAGCAGT CGGGGTTGCG CGTGTTACAC AGCCCGCCTG CCGTGCGCAG GGTCGACAGC 
ATCACGGCGG CAGGTGGTGA GGGTCCCTTT CCCACAAGCA GAGCCAGAGG GAGCCCGGGA 
GACACCAAGG GAGGCCCTCC AGAACCCATG CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG 
CACTCCCGGG ACTATGTGGA GGGGGCACGG CGCCCCCTTG ATAGTCCCCT CTGTACCTCC 
CTGGGGTTTG CCTCCCCACT GCACAGCCTG GAGATGTCCA AGAACTTGAG TGATGACATG 
AAGGAGGTGG CCTTCTCTGT CAGGAATGCC ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC 
AAGGACATGG CCTGCCAGAC CAATGGGTCC CGGACGATGG GGACCCAGAC TGTTCAGACC 
ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG CGTGGCAGCG GTGTCACCAG CAGCCCCCAC 
AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT GCTACACCCG TGTCGTCTCC TTCCCGGAGC 
CTTAGGAGCA GACAGGTGGC CCCTGCCATC GAGAAGGTGC AGGCCAAGTT TGAACGCACA 
TGCTGCTCCC CCAAGTATGG TTCTCCCAAG CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC 
CAGCCAAATA ACAGGACGTC ACCAGGGATG GCCCAGAAAG GGTACAGTGA GTCAGCCTGG 
GCCCGCTCCA CCACCACAAG GGAGAGCCCC GTGCACACCA CCATTAATGA TGGCCTCTCC 
AGCCTCTTCA ACATCATTGA CCACAGCCCC GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG 
CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG CCCCGACCAG AGCTGGGCCC AGGCCAGGAA 
ACAGGCACCA ATTCCCGAGG AAGGTCGCCT AGCCCCATTG GGGTGGGGTC AGAGATGTGC 
AGGGAGGAAG GGGGAGAGGG CACGCCAGTG AAGCAGGACT TATCTGCTCC CCCTGGCTAC 
ACGCTCACTG AGAACGTGGC CCGGATCCTC AACAAGAAGC TGCTGGAACA TGCCTTAAAG 
GAGGAGAGGA GGCAGGCTGC CCACGGGCCC CCGGGTCTCC ACAGTGACAG CCACTCGCTG 
GGGGACACAG CCGAGCCAGG GCCCATGGAG GAACTACCTT GTTCTGCACT AGCTCCATCC 
CTAGAGCCCT GCTTCTCCAG GCCCGAGAGA CCAGCAAACC GTCGCCCTCC GTCCCGTTGG 
GCCCCACATT CCCCCACTGC CTCACAGCCT CAGTCACCCG GAGACCCGAC GTCCTTGGAG 
GAGCATGGTG GCGAGGAGCC GCCCGAGGAG CAGCCACACC GAGATGCAAG CTTGCATGGA 
TTATCACAGT ATAATTCACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523942.1
CDS	86..6229
Translation	METLNGPAGGGAPDAKLQPPGQHHRHHHLHPVAERRRLHRAPSPARPFLKDLHARPAAPGPAVPSSGRAPAPAAPRSPNLAGKAPPSPGSLAAPGRLSRRSGGVPGAKDKPPPGAGARAAGGAKAALGSRRAARVAPAEPLSRAGKPPGAEPPSAAAKGRKAKRGSRAPPARTVGPPTPAARIPAVTLAVTSVAGSPARCSRISHTDSSSDLSDCPSEPLSDEQRLLPAASSDAESGTGSSDREPPRGAPTPSPAARGAPPGSPEPPALLAAPLAAGACPGGRSIPSGVSGGFAGPGVAEDVRGRSPPERPVPGTPKEPSLGEQSRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQQNGGMERPGNCRPATKTQRKLAPRRKDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQNCCGYPRINIEEETLGFTRLPAGSTVKTLKSLGLQRLELEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL

Target ORF information:

RefSeq Version	XM_011525640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525640.2

ORF Insert Sequence:

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGCAG 
AATGGTGGAA TGGAGCGCCC TGGGAACTGC AGGCCCGCAA CCAAAACCCA AAGGAAGCTG 
GCGCCCCGGC GCAAGGATGA CAGTGCCGAT TTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAAGCCTTCC TGATGCGCAA AAAGATGGCC AAGCTAGGAA GGGAGAAGGA CGAGCTGGAG 
CAGGAGCTCC AGAAGTACAA GTCCCTCTAT GGGGATGTGG ACAGTCCCCT GCCCACGGGG 
GAAGCAGGCG GGCCCCCCAG CACCCGGGAG GCCGAGCTGA AGCTGCGGCT AAAGCTGGTG 
GAGGAGGAAG CCAACATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAACCGTGGC 
CTCAAGGCAG AGATGGAGGA CATGCGGGGC CAGCAGGAGC GGGAGGGCCC GGGTCGGGAC 
CACGCACCCA GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC CACTGAGCTC 
CGCCGCCACC TGCAGTTTGT AGAAGAGGAA GCGGAGTTGC TCCGGAGGTC CATCTCCGAG 
ATCGAAGACC ACAACCGGCA ACTGACCCAC GAGCTCAGCA AGTTTAAGTT TGAGCCTCCC 
CGGGAGCCGG GCTGGCTAGG AGAGGGTGCA AGTCCTGGTG CCGGGGGTGG GGCCCCCCTG 
CAGGAGGAGC TGAAGTCAGC CAGGCTGCAG ATCAGCGAGC TCAGCGGCAA GGTGCTCAAA 
CTGCAGCACG AGAACCACGC GCTGCTGTCC AACATCCAGC GCTGCGACCT GGCAGCCCAC 
CTGGGGCTGC GTGCCCCCAG TCCCCGGGAC AGCGATGCCG AGAGTGATGC GGGCAAGAAG 
GAGAGTGATG GGGAGGAGAG CCGCCTGCCC CAGCCCAAGC GGGAAGGGCC TGTTGGCGGG 
GAGAGTGACT CGGAGGAAAT GTTTGAGAAG ACGTCGGGCT TCGGGAGCGG GAAGCCATCG 
GAGGCCAGCG AGCCATGCCC CACGGAGCTC CTGAAGGCCC GGGAGGACTC TGAGTACCTA 
GTGACCCTAA AACACGAGGC CCAGCGGCTA GAGCGGACGG TGGAGCGCCT CATCACGGAC 
ACCGACAGCT TCCTCCATGA TGCGGGGCTG CGGGGTGGTG CGCCCTTACC GGGGCCTGGC 
CTCCAGGGCG AAGAGGAGCA GGGTGAGGGG GACCAGCAGG AGCCCCAGCT GCTGGGGACC 
ATCAACGCCA AGATGAAGGC TTTCAAGAAA GAGCTGCAGG CCTTCCTGGA GCAGGTGAAC 
CGCATTGGGG ATGGCCTATC CCCCTTGCCC CACCTCACAG AGTCCTCTAG CTTCCTCTCC 
ACTGTGACTT CCGTGTCCCG GGACTCCCCC ATCGGGAACC TGGGGAAGGA GCTGGGCCCA 
GACTTGCAGT CCAGACTGAA AGAGCAGCTG GAGTGGCAGC TCGGGCCGGC CCGAGGGGAC 
GAGCGGGAGA GCCTGCGCCT CCGAGCCGCG CGGGAGCTGC ACCGCCGCGC AGACGGGGAC 
ACCGGGAGCC ACGGGCTGGG AGGCCAGACC TGCTTCAGCC TGGAGCTCAG GGGCCCCCCC 
GTTTTACCTG AGCAGAGTGT ATCCATAGAG GAGCTACAGG GTCAGCTCGT GCAGGCGGCC 
AGACTGCATC AAGAGGAGAC AGAGACATTT ACAAACAAGA TCCATAAGAT GGAGGAGGAG 
CACCTCTATG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAAAACACC 
CTCCATGAGC GAACCTGGAG TGATGAGAAG AATCTGATGC AGCAGGAGCT CCGGTCCTTG 
AAGCAGAACA TTTTCCTCTT CTACGTCAAA CTCAGGTGGC TGCTGAAACA CTGGCGGCAA 
GGGAAGCAGA TGGAGGAGGA AGGAGAGGAG TTCACTGAGG GTGAACATCC AGAGACCCTC 
TCCAGGCTCG GGGAGCTTGG AGTCCAGGGG GGTCACCAGG CGGATGGCCC AGACCACGAC 
AGTGACCGAG GCTGTGGCTT TCCAGTGGGG GAGCACTCCC CACACTCCCG GGTGCAGATT 
GGAGATCACA GCTTGCGGCT GCAGACCGCG GACAGGGGAC AGCCCCACAA ACAGGTGGTG 
GAAAACCAGC AGCTGTTCAG CGCCTTCAAG GCCTTGCTGG AGGACTTCCG TGCGGAGCTG 
CGGGAGGATG AGCGTGCCCG ACTACGGCTG CAGCAGCAAT ATGCCAGCGA CAAGGCGGCC 
TGGGACGTGG AGTGGGCCGT GCTCAAGTGC CGTCTGGAAC AGAATTGTTG TGGATATCCC 
AGAATTAACA TTGAGGAGGA GACTTTAGGC TTCACCAGGC TGCCAGCTGG GTCCACGGTA 
AAAACGTTGA AGAGCCTTGG GTTGCAGAGA TTGGAGCTGG AAGAGAAGAC TGAGAACAAG 
TTGGGAGAAC TAGGCTCCTC CGCTGAGAGC AAGGGGGCCT TGAAGAAGGA GAGAGAGGTG 
CACCAGAAGC TCCTGGCAGA CAGTCACAGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGCGAGA AGAACTGGAA CCGGGAGAAG GTGGAACTTC TCGACCGCCT GGACAGAGAT 
CGGCAGGAGT GGGAGCGGCA GAAGAAGGAA TTCTTGTGGA GGATAGAGCA GGGAAGCCTC 
CGCATGCCCC GTCCAGTGGC CATGTGGCCT TGTGCAGATG CTGACTCCAT CCCGTTTGAA 
GACCGGCCGC TGTCCAAGCT GAAGGAGTCG GACAGGTGCT CGGCCAGTGA GAATCTCTAC 
CTGGATGCCT TGTCCCTGGA TGACGAGCCA GAAGAGCCAC CAGCCCACAG GCCCGAGAGG 
GAGTTCAGGA ACCGCCTCCC TGAGGAAGAA GAAAATCACA AAGGAAATCT TCAAAGGGCG 
GTGTCCGTGT CCTCCATGTC TGAGTTCCAG CGTCTAATGG ACATCTCCCC CTTCCTGCCT 
GAGAAGGGCC TGCCGTCCAC CAGCAGCAAG GAGGATGTCA CCCCACCCCT GTCTCCAGAC 
GACCTCAAGT ACATCGAGGA GTTCAACAAG AGCTGGGACT ACACACCCAA CAGGGGCCAC 
AATGGTGGGG GGCCGGACCT TTGGGCCGAC AGGACCGAGG TGGGGCGGGC AGGGCACGAG 
GACAGCACAG AGCCTTTCCC CGACTCCTCC TGGTACCTAA CCACAAGTGT CACCATGACC 
ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CACTGCGGAG CCACGTCCTC 
ACCGAGCAGT CGGGGTTGCG CGTGTTACAC AGCCCGCCTG CCGTGCGCAG GGTCGACAGC 
ATCACGGCGG CAGGTGGTGA GGGTCCCTTT CCCACAAGCA GAGCCAGAGG GAGCCCGGGA 
GACACCAAGG GAGGCCCTCC AGAACCCATG CTCAGCAGGT GGCCTTGCAC CTCCCCCAGG 
CACTCCCGGG ACTATGTGGA GGGGGCACGG CGCCCCCTTG ATAGTCCCCT CTGTACCTCC 
CTGGGGTTTG CCTCCCCACT GCACAGCCTG GAGATGTCCA AGAACTTGAG TGATGACATG 
AAGGAGGTGG CCTTCTCTGT CAGGAATGCC ATCTGCTCCG GCCCTGGCGA GCTGCAAGTC 
AAGGACATGG CCTGCCAGAC CAATGGGTCC CGGACGATGG GGACCCAGAC TGTTCAGACC 
ATCAGTGTGG GCTTGCAGAC TGAAGCCCTG CGTGGCAGCG GTGTCACCAG CAGCCCCCAC 
AAGTGTCTCA CTCCAAAGGC TGGGGGCGGT GCTACACCCG TGTCGTCTCC TTCCCGGAGC 
CTTAGGAGCA GACAGGTGGC CCCTGCCATC GAGAAGGTGC AGGCCAAGTT TGAACGCACA 
TGCTGCTCCC CCAAGTATGG TTCTCCCAAG CTGCAGAGGA AGCCCCTCCC CAAAGCCGAC 
CAGCCAAATA ACAGGACGTC ACCAGGGATG GCCCAGAAAG GGTACAGTGA GTCAGCCTGG 
GCCCGCTCCA CCACCACAAG GGAGAGCCCC GTGCACACCA CCATTAATGA TGGCCTCTCC 
AGCCTCTTCA ACATCATTGA CCACAGCCCC GTGGTGCAGG ACCCCTTCCA GAAGGGGCTG 
CGGGCCGGCA GTCGGTCTCG CTCAGCAGAG CCCCGACCAG AGCTGGGCCC AGGCCAGGAA 
ACAGGCACCA ATTCCCGAGG AAGGTCGCCT AGCCCCATTG GGGTGGGGTC AGAGATGTGC 
AGGGAGGAAG GGGGAGAGGG CACGCCAGTG AAGCAGGACT TATCTGCTCC CCCTGGCTAC 
ACGCTCACTG AGAACGTGGC CCGGATCCTC AACAAGAAGC TGCTGGAACA TGCCTTAAAG 
GAGGAGAGGA GGCAGGCTGC CCACGGGCCC CCGGGTCTCC ACAGTGACAG CCACTCGCTG 
GGGGACACAG CCGAGCCAGG GCCCATGGAG GAACTACCTT GTTCTGCACT AGCTCCATCC 
CTAGAGCCCT GCTTCTCCAG GCCCGAGAGA CCAGCAAACC GTCGCCCTCC GTCCCGTTGG 
GCCCCACATT CCCCCACTGC CTCACAGCCT CAGTCACCCG GAGACCCGAC GTCCTTGGAG 
GAGCATGGTG GCGAGGAGCC GCCCGAGGAG CAGCCACACC GAGATGCAAG CTTGCATGGA 
TTATCACAGT ATAATTCACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_011525641.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523943.1 Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

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CloneID	OHu65814
Clone ID Related Accession (Same CDS sequence)	XM_011525641.2
Accession Version	XM_011525641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule cross-linking factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523943.1
CDS	86..5926
Translation	METLNGPAGGGAPDAKLQPPGQHHRHHHLHPVAERRRLHRAPSPARPFLKDLHARPAAPGPAVPSSGRAPAPAAPRSPNLAGKAPPSPGSLAAPGRLSRRSGGVPGAKDKPPPGAGARAAGGAKAALGSRRAARVAPAEPLSRAGKPPGAEPPSAAAKGRKAKRGSRAPPARTVGPPTPAARIPAVTLAVTSVAGSPARCSRISHTDSSSDLSDCPSEPLSDEQRLLPAASSDAESGTGSSDREPPRGAPTPSPAARGAPPGSPEPPALLAAPLAAGACPGGRSIPSGVSGGFAGPGVAEDVRGRSPPERPVPGTPKEPSLGEQSRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELKTVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRSTREMYKEKKTFNQDDSADLRCQLQFAKEEAFLMRKKMAKLGREKDELEQELQKYKSLYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRGQQEREGPGRDHAPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPPREPGWLGEGASPGAGGGAPLQEELKSARLQISELSGKVLKLQHENHALLSNIQRCDLAAHLGLRAPSPRDSDAESDAGKKESDGEESRLPQPKREGPVGGESDSEEMFEKTSGFGSGKPSEASEPCPTELLKAREDSEYLVTLKHEAQRLERTVERLITDTDSFLHDAGLRGGAPLPGPGLQGEEEQGEGDQQEPQLLGTINAKMKAFKKELQAFLEQVNRIGDGLSPLPHLTESSSFLSTVTSVSRDSPIGNLGKELGPDLQSRLKEQLEWQLGPARGDERESLRLRAARELHRRADGDTGSHGLGGQTCFSLELRGPPVLPEQSVSIEELQGQLVQAARLHQEETETFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDEKNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEEFTEGEHPETLSRLGELGVQGGHQADGPDHDSDRGCGFPVGEHSPHSRVQIGDHSLRLQTADRGQPHKQVVENQQLFSAFKALLEDFRAELREDERARLRLQQQYASDKAAWDVEWAVLKCRLEQLEEKTENKLGELGSSAESKGALKKEREVHQKLLADSHSLVMDLRWQIHHSEKNWNREKVELLDRLDRDRQEWERQKKEFLWRIEQLQKENSPRRGGSFLCDQKDGNVRPFPHQGSLRMPRPVAMWPCADADSIPFEDRPLSKLKESDRCSASENLYLDALSLDDEPEEPPAHRPEREFRNRLPEEEENHKGNLQRAVSVSSMSEFQRLMDISPFLPEKGLPSTSSKEDVTPPLSPDDLKYIEEFNKSWDYTPNRGHNGGGPDLWADRTEVGRAGHEDSTEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSHVLTEQSGLRVLHSPPAVRRVDSITAAGGEGPFPTSRARGSPGDTKGGPPEPMLSRWPCTSPRHSRDYVEGARRPLDSPLCTSLGFASPLHSLEMSKNLSDDMKEVAFSVRNAICSGPGELQVKDMACQTNGSRTMGTQTVQTISVGLQTEALRGSGVTSSPHKCLTPKAGGGATPVSSPSRSLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGMAQKGYSESAWARSTTTRESPVHTTINDGLSSLFNIIDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_011525641.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525641.2

ORF Insert Sequence:

ATGGAGACAC TGAACGGCCC CGCGGGCGGA GGTGCCCCGG ACGCGAAGCT GCAGCCGCCC 
GGCCAGCACC ACCGCCACCA CCACCTCCAC CCGGTGGCCG AAAGGCGGCG GCTGCACCGT 
GCGCCCTCGC CCGCCAGACC CTTCCTCAAG GACCTGCACG CCCGGCCCGC CGCGCCCGGC 
CCGGCCGTCC CCTCCTCGGG CCGAGCCCCG GCTCCCGCCG CCCCGCGCTC GCCCAACCTC 
GCGGGCAAAG CGCCGCCCTC GCCGGGGTCC CTGGCCGCGC CCGGCCGCCT CTCTCGGCGC 
AGTGGCGGCG TCCCGGGCGC GAAGGACAAG CCCCCGCCGG GCGCCGGGGC CAGAGCGGCG 
GGCGGTGCCA AGGCTGCCCT GGGAAGCAGG AGGGCGGCGC GCGTGGCGCC CGCGGAGCCG 
CTGTCCCGGG CTGGGAAGCC TCCCGGAGCC GAGCCGCCGT CTGCCGCTGC CAAGGGCCGC 
AAAGCCAAGC GCGGCTCCCG GGCGCCACCC GCCCGCACCG TCGGCCCCCC GACCCCGGCC 
GCCCGGATCC CTGCCGTGAC CCTCGCGGTG ACCTCGGTGG CCGGGTCCCC GGCCCGCTGC 
TCGCGCATCA GCCACACGGA CAGCAGCTCC GACCTCTCTG ACTGCCCCTC CGAACCCCTG 
TCCGACGAGC AGCGCCTGCT CCCTGCCGCC AGCAGCGACG CCGAATCCGG CACGGGCTCC 
AGCGACCGTG AACCCCCACG AGGAGCGCCG ACGCCGAGCC CCGCAGCCCG AGGAGCGCCG 
CCGGGCAGCC CCGAGCCCCC AGCGCTCCTC GCCGCGCCCC TCGCCGCGGG CGCCTGTCCC 
GGGGGCCGGA GCATCCCGAG CGGGGTTTCG GGGGGTTTCG CGGGGCCCGG CGTGGCGGAG 
GATGTCCGGG GGCGCTCGCC ACCGGAGCGA CCAGTCCCCG GGACCCCCAA GGAGCCCAGC 
CTGGGCGAGC AGTCTCGGCT CGTGCCAGCG GCGGAGGAAG AAGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TAGATGAACT CCGTGCTGAG 
ATGGAAGAGA TGAGAGACAG TTATTTAGAG GAAGATGTTT ACCAGCTGCA GGAACTTCGG 
CGAGAACTGG ACCGCGCTAA TAAAAACTGC CGAATCCTGC AGTACCGTCT TCGGAAAGCC 
GAGCAGAAAA GCCTGAAAGT GGCTGAGACG GGTCAGGTGG ATGGTGAGCT TATTCGAAGC 
CTGGAGCAGG ACTTGAAGGT AGCCAAAGAT GTATCTGTCA GATTGCACCA CGAACTTAAG 
ACGGTGGAGG AAAAGCGCGC TAAAGCTGAG GATGAAAACG AAACTCTCCG ACAGCAGATG 
ATTGAAGTGG AAATATCCAA ACAGGCCCTC CAGAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTAAAAA GAAGAAGCAC TCGGGAAATG TACAAGGAGA AGAAAACCTT TAACCAGGAT 
GACAGTGCCG ATTTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAAGCCTT CCTGATGCGC 
AAAAAGATGG CCAAGCTAGG AAGGGAGAAG GACGAGCTGG AGCAGGAGCT CCAGAAGTAC 
AAGTCCCTCT ATGGGGATGT GGACAGTCCC CTGCCCACGG GGGAAGCAGG CGGGCCCCCC 
AGCACCCGGG AGGCCGAGCT GAAGCTGCGG CTAAAGCTGG TGGAGGAGGA AGCCAACATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAACCGTG GCCTCAAGGC AGAGATGGAG 
GACATGCGGG GCCAGCAGGA GCGGGAGGGC CCGGGTCGGG ACCACGCACC CAGCATTCCT 
ACCTCACCCT TCGGTGACTC CCTGGAGTCC TCCACTGAGC TCCGCCGCCA CCTGCAGTTT 
GTAGAAGAGG AAGCGGAGTT GCTCCGGAGG TCCATCTCCG AGATCGAAGA CCACAACCGG 
CAACTGACCC ACGAGCTCAG CAAGTTTAAG TTTGAGCCTC CCCGGGAGCC GGGCTGGCTA 
GGAGAGGGTG CAAGTCCTGG TGCCGGGGGT GGGGCCCCCC TGCAGGAGGA GCTGAAGTCA 
GCCAGGCTGC AGATCAGCGA GCTCAGCGGC AAGGTGCTCA AACTGCAGCA CGAGAACCAC 
GCGCTGCTGT CCAACATCCA GCGCTGCGAC CTGGCAGCCC ACCTGGGGCT GCGTGCCCCC 
AGTCCCCGGG ACAGCGATGC CGAGAGTGAT GCGGGCAAGA AGGAGAGTGA TGGGGAGGAG 
AGCCGCCTGC CCCAGCCCAA GCGGGAAGGG CCTGTTGGCG GGGAGAGTGA CTCGGAGGAA 
ATGTTTGAGA AGACGTCGGG CTTCGGGAGC GGGAAGCCAT CGGAGGCCAG CGAGCCATGC 
CCCACGGAGC TCCTGAAGGC CCGGGAGGAC TCTGAGTACC TAGTGACCCT AAAACACGAG 
GCCCAGCGGC TAGAGCGGAC GGTGGAGCGC CTCATCACGG ACACCGACAG CTTCCTCCAT 
GATGCGGGGC TGCGGGGTGG TGCGCCCTTA CCGGGGCCTG GCCTCCAGGG CGAAGAGGAG 
CAGGGTGAGG GGGACCAGCA GGAGCCCCAG CTGCTGGGGA CCATCAACGC CAAGATGAAG 
GCTTTCAAGA AAGAGCTGCA GGCCTTCCTG GAGCAGGTGA ACCGCATTGG GGATGGCCTA 
TCCCCCTTGC CCCACCTCAC AGAGTCCTCT AGCTTCCTCT CCACTGTGAC TTCCGTGTCC 
CGGGACTCCC CCATCGGGAA CCTGGGGAAG GAGCTGGGCC CAGACTTGCA GTCCAGACTG 
AAAGAGCAGC TGGAGTGGCA GCTCGGGCCG GCCCGAGGGG ACGAGCGGGA GAGCCTGCGC 
CTCCGAGCCG CGCGGGAGCT GCACCGCCGC GCAGACGGGG ACACCGGGAG CCACGGGCTG 
GGAGGCCAGA CCTGCTTCAG CCTGGAGCTC AGGGGCCCCC CCGTTTTACC TGAGCAGAGT 
GTATCCATAG AGGAGCTACA GGGTCAGCTC GTGCAGGCGG CCAGACTGCA TCAAGAGGAG 
ACAGAGACAT TTACAAACAA GATCCATAAG ATGGAGGAGG AGCACCTCTA TGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAAAACA CCCTCCATGA GCGAACCTGG 
AGTGATGAGA AGAATCTGAT GCAGCAGGAG CTCCGGTCCT TGAAGCAGAA CATTTTCCTC 
TTCTACGTCA AACTCAGGTG GCTGCTGAAA CACTGGCGGC AAGGGAAGCA GATGGAGGAG 
GAAGGAGAGG AGTTCACTGA GGGTGAACAT CCAGAGACCC TCTCCAGGCT CGGGGAGCTT 
GGAGTCCAGG GGGGTCACCA GGCGGATGGC CCAGACCACG ACAGTGACCG AGGCTGTGGC 
TTTCCAGTGG GGGAGCACTC CCCACACTCC CGGGTGCAGA TTGGAGATCA CAGCTTGCGG 
CTGCAGACCG CGGACAGGGG ACAGCCCCAC AAACAGGTGG TGGAAAACCA GCAGCTGTTC 
AGCGCCTTCA AGGCCTTGCT GGAGGACTTC CGTGCGGAGC TGCGGGAGGA TGAGCGTGCC 
CGACTACGGC TGCAGCAGCA ATATGCCAGC GACAAGGCGG CCTGGGACGT GGAGTGGGCC 
GTGCTCAAGT GCCGTCTGGA ACAGCTGGAA GAGAAGACTG AGAACAAGTT GGGAGAACTA 
GGCTCCTCCG CTGAGAGCAA GGGGGCCTTG AAGAAGGAGA GAGAGGTGCA CCAGAAGCTC 
CTGGCAGACA GTCACAGCCT GGTCATGGAC CTGCGCTGGC AGATCCATCA CAGCGAGAAG 
AACTGGAACC GGGAGAAGGT GGAACTTCTC GACCGCCTGG ACAGAGATCG GCAGGAGTGG 
GAGCGGCAGA AGAAGGAATT CTTGTGGAGG ATAGAGCAGT TGCAGAAAGA GAACAGTCCC 
CGGAGAGGTG GCAGTTTCCT CTGTGATCAA AAAGACGGCA ACGTTCGCCC CTTTCCCCAC 
CAGGGAAGCC TCCGCATGCC CCGTCCAGTG GCCATGTGGC CTTGTGCAGA TGCTGACTCC 
ATCCCGTTTG AAGACCGGCC GCTGTCCAAG CTGAAGGAGT CGGACAGGTG CTCGGCCAGT 
GAGAATCTCT ACCTGGATGC CTTGTCCCTG GATGACGAGC CAGAAGAGCC ACCAGCCCAC 
AGGCCCGAGA GGGAGTTCAG GAACCGCCTC CCTGAGGAAG AAGAAAATCA CAAAGGAAAT 
CTTCAAAGGG CGGTGTCCGT GTCCTCCATG TCTGAGTTCC AGCGTCTAAT GGACATCTCC 
CCCTTCCTGC CTGAGAAGGG CCTGCCGTCC ACCAGCAGCA AGGAGGATGT CACCCCACCC 
CTGTCTCCAG ACGACCTCAA GTACATCGAG GAGTTCAACA AGAGCTGGGA CTACACACCC 
AACAGGGGCC ACAATGGTGG GGGGCCGGAC CTTTGGGCCG ACAGGACCGA GGTGGGGCGG 
GCAGGGCACG AGGACAGCAC AGAGCCTTTC CCCGACTCCT CCTGGTACCT AACCACAAGT 
GTCACCATGA CCACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCACTGCGG 
AGCCACGTCC TCACCGAGCA GTCGGGGTTG CGCGTGTTAC ACAGCCCGCC TGCCGTGCGC 
AGGGTCGACA GCATCACGGC GGCAGGTGGT GAGGGTCCCT TTCCCACAAG CAGAGCCAGA 
GGGAGCCCGG GAGACACCAA GGGAGGCCCT CCAGAACCCA TGCTCAGCAG GTGGCCTTGC 
ACCTCCCCCA GGCACTCCCG GGACTATGTG GAGGGGGCAC GGCGCCCCCT TGATAGTCCC 
CTCTGTACCT CCCTGGGGTT TGCCTCCCCA CTGCACAGCC TGGAGATGTC CAAGAACTTG 
AGTGATGACA TGAAGGAGGT GGCCTTCTCT GTCAGGAATG CCATCTGCTC CGGCCCTGGC 
GAGCTGCAAG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACGAT GGGGACCCAG 
ACTGTTCAGA CCATCAGTGT GGGCTTGCAG ACTGAAGCCC TGCGTGGCAG CGGTGTCACC 
AGCAGCCCCC ACAAGTGTCT CACTCCAAAG GCTGGGGGCG GTGCTACACC CGTGTCGTCT 
CCTTCCCGGA GCCTTAGGAG CAGACAGGTG GCCCCTGCCA TCGAGAAGGT GCAGGCCAAG 
TTTGAACGCA CATGCTGCTC CCCCAAGTAT GGTTCTCCCA AGCTGCAGAG GAAGCCCCTC 
CCCAAAGCCG ACCAGCCAAA TAACAGGACG TCACCAGGGA TGGCCCAGAA AGGGTACAGT 
GAGTCAGCCT GGGCCCGCTC CACCACCACA AGGGAGAGCC CCGTGCACAC CACCATTAAT 
GATGGCCTCT CCAGCCTCTT CAACATCATT GACCACAGCC CCGTGGTGCA GGACCCCTTC 
CAGAAGGGGC TGCGGGCCGG CAGTCGGTCT CGCTCAGCAG AGCCCCGACC AGAGCTGGGC 
CCAGGCCAGG AAACAGGCAC CAATTCCCGA GGAAGGTCGC CTAGCCCCAT TGGGGTGGGG 
TCAGAGATGT GCAGGGAGGA AGGGGGAGAG GGCACGCCAG TGAAGCAGGA CTTATCTGCT 
CCCCCTGGCT ACACGCTCAC TGAGAACGTG GCCCGGATCC TCAACAAGAA GCTGCTGGAA 
CATGCCTTAA AGGAGGAGAG GAGGCAGGCT GCCCACGGGC CCCCGGGTCT CCACAGTGAC 
AGCCACTCGC TGGGGGACAC AGCCGAGCCA GGGCCCATGG AGAACCAAAC TGTCTTGCTA 
ACTGCCCCCT GGGGACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular basis of the microtubule-regulating activity of microtubule crosslinking factor 1.
PloS one12(8)e0182641(2017)
Kader MA,Satake T,Yoshida M,Hayashi I,Suzuki A

A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.
The FEBS journal283(19)3662-3671(2016 10)
Hyodo T,Ito S,Asano-Inami E,Chen D,Senga T

MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane.
Nature communications55266(2014 Nov)
Sato Y,Hayashi K,Amano Y,Takahashi M,Yonemura S,Hayashi I,Hirose H,Ohno S,Suzuki A

The novel PAR-1-binding protein MTCL1 has crucial roles in organizing microtubules in polarizing epithelial cells.
Journal of cell science126(Pt 20)4671-83(2013 Oct)
Sato Y,Akitsu M,Amano Y,Yamashita K,Ide M,Shimada K,Yamashita A,Hirano H,Arakawa N,Maki T,Hayashi I,Ohno S,Suzuki A

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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MTCL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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