COBL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COBL
Entrez Gene ID	23242
Full Name	cordon-bleu WH2 repeat protein
General protein information	Preferred Names cordon-bleu WH2 repeat protein Names protein cordon-bleu
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p12.1
Summary	This gene encodes a protein that contains WH2 domains (WASP, Wiskott-Aldrich syndrome protein, homology domain-2) that interact with actin. The encoded actin regulator protein is required for growth and assembly of brush border microvilli that play a role in maintaining intestinal homeostasis. A similar protein in mouse functions in midbrain neural tube closure. A pseudogene of this gene is located on chromosome X. [provided by RefSeq, Oct 2016].
Disorder MIM:	610317

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346442.2	NP_001333371.1	protein cordon-bleu isoform e
NM_015198.5	NP_056013.2	protein cordon-bleu isoform b
NM_001346441.2	NP_001333370.1	protein cordon-bleu isoform d
XM_005271751.1	XP_005271808.1	protein cordon-bleu isoform X4
XM_011515238.1	XP_011513540.1	protein cordon-bleu isoform X8
XM_011515239.1	XP_011513541.1	protein cordon-bleu isoform X9
XM_011515241.1	XP_011513543.1	protein cordon-bleu isoform X11
NM_001287438.2	NP_001274367.1	protein cordon-bleu isoform c
NM_001346444.1	NP_001333373.1	protein cordon-bleu isoform g
XM_011515234.1	XP_011513536.1	protein cordon-bleu isoform X1
XM_005271750.1	XP_005271807.1	protein cordon-bleu isoform X3
XM_011515236.1	XP_011513538.1	protein cordon-bleu isoform X5
XM_017011898.1	XP_016867387.1	protein cordon-bleu isoform X7
NM_015198.4	NP_056013.2	protein cordon-bleu isoform b
NM_001346442.1	NP_001333371.1	protein cordon-bleu isoform e
NM_001287438.3	NP_001274367.1	protein cordon-bleu isoform c
NM_001346443.2	NP_001333372.1	protein cordon-bleu isoform f
NM_001346441.1	NP_001333370.1	protein cordon-bleu isoform d
NM_001346443.1	NP_001333372.1	protein cordon-bleu isoform f
NM_001287436.2	NP_001274365.1	protein cordon-bleu isoform a
XM_011515235.1	XP_011513537.1	protein cordon-bleu isoform X2
XM_011515237.1	XP_011513539.1	protein cordon-bleu isoform X6
XM_011515240.1	XP_011513542.1	protein cordon-bleu isoform X10

Pathways from BioSystems

Homologies

	COBL	XP_001082572.2
Bos taurus (cattle)	COBL	XP_003585983.2
Gallus gallus (chicken)	COBL	XP_004935203.1
Pan troglodytes (chimpanzee)	COBL	XP_003318477.1
Rattus norvegicus (Norway rat)	Cobl	NP_001100706.1
Xenopus tropicalis (tropical clawed frog)	cobl	NP_001135515.1
Homo sapiens (human)	COBL	NP_001274365.1
Canis lupus familiaris (dog)	COBL	XP_005631063.1
Mus musculus (house mouse)	Cobl	NP_766084.3

Gene Ontology
Bibliography

Related articles in PubMed

Two novel loci, COBL and SLC10A2, for Alzheimer's disease in African Americans.
Mez J, Chung J, Jun G, Kriegel J, Bourlas AP, Sherva R, Logue MW, Barnes LL, Bennett DA, Buxbaum JD, Byrd GS, Crane PK, Ertekin-Taner N, Evans D, Fallin MD, Foroud T, Goate A, Graff-Radford NR, Hall KS, Kamboh MI, Kukull WA, Larson EB, Manly JJ, , Haines JL, Mayeux R, Pericak-Vance MA, Schellenberg GD, Lunetta KL, Farrer LA
Alzheimer's & dementia : the journal of the Alzheimer's Association13(2)119-129(2017 Feb)

COBL is a novel hotspot for IKZF1 deletions in childhood acute lymphoblastic leukemia.
Lopes BA, Meyer C, Barbosa TC, Zur Stadt U, Horstmann M, Venn NC, Heatley S, White DL, Sutton R, Pombo-de-Oliveira MS, Marschalek R, Emerenciano M
Oncotarget7(33)53064-53073(2016 Aug)

The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.
Hou W, Izadi M, Nemitz S, Haag N, Kessels MM, Qualmann B
PLoS biology13(9)e1002233(2015)

Mutagenetic and electron microscopy analysis of actin filament severing by Cordon-Bleu, a WH2 domain protein.
Jiao Y, Walker M, Trinick J, Pernier J, Montaville P, Carlier MF
Cytoskeleton (Hoboken, N.J.)71(3)170-83(2014 Mar)

Association of granulomatosis with polyangiitis (Wegener's) with HLA-DPB1*04 and SEMA6A gene variants: evidence from genome-wide analysis.
Xie G, Roshandel D, Sherva R, Monach PA, Lu EY, Kung T, Carrington K, Zhang SS, Pulit SL, Ripke S, Carette S, Dellaripa PF, Edberg JC, Hoffman GS, Khalidi N, Langford CA, Mahr AD, St Clair EW, Seo P, Specks U, Spiera RF, Stone JH, Ytterberg SR, Raychaudhuri S, de Bakker PI, Farrer LA, Amos CI, Merkel PA, Siminovitch KA
Arthritis and rheumatism65(9)2457-68(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two single-nucleotide polymorphisms at novel loci, rs112404845 (P = 3.8 x 10-8), upstream of COBL, and rs16961023 (P = 4.6 x 10-8), downstream of SLC10A2, obtained genome-wide significant evidence of association with the posterior liability of late-onset Alzheimer's disease in African Americans.
Title: Two novel loci, COBL and SLC10A2, for Alzheimer's disease in African Americans.

Data show that six patients had large interstitial deletions starting within intronic regions of COBL at diagnosis, which is ~611 Kb downstream of IKZF1, suggesting that COBL is a hotspot for IKZF1 deletion (DeltaIKZF1).
Title: COBL is a novel hotspot for IKZF1 deletions in childhood acute lymphoblastic leukemia.

Ca2+/Calmodulin modulates Cobl's actin binding properties and furthermore promotes Cobl's previously identified interactions with the membrane-shaping F-BAR protein syndapin I, which accumulated with Cobl at nascent dendritic protrusion sites.
Title: The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.

Filament severing displayed the greatest stringency and was abolished in all mutated forms of Cobl-KAB.
Title: Mutagenetic and electron microscopy analysis of actin filament severing by Cordon-Bleu, a WH2 domain protein.

Cordon-Bleu works as a dynamizer of actin assembly by combining many properties of profilin with weak filament nucleating and powerful filament severing activities and sequestration of ADP-actin, which generate oscillatory polymerization kinetics.
Title: Cordon-Bleu uses WH2 domains as multifunctional dynamizers of actin filament assembly.

The following COBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06114	NM_001287438.3 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu101284	NM_001346444.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu101282	NM_001346441.2 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu101283	NM_001346443.2 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu97125	XM_017011898.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu06113	NM_001287436.2 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu06870	NM_015198.5 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu41131	NM_001346442.2 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu41126	XM_005271751.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu41132	XM_011515241.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65779	XM_011515236.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65780	XM_011515237.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65781	XM_011515238.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65782	XM_011515239.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65783	XM_011515240.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu41125	XM_005271750.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu65777	XM_011515234.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu65778	XM_011515235.1 Latest version!	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu06870	NM_015198.4	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu41131	NM_001346442.1	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu06114	NM_001287438.2	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu101282	NM_001346441.1	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu101283	NM_001346443.1	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COBL ( NM_001287438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274367.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06114
Clone ID Related Accession (Same CDS sequence)	NM_001287438.2 , NM_001287438.3
Accession Version	NM_001287438.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC111496.1, BC094695.1 and DA245843.1. On Oct 13, 2016 this sequence version replaced NM_001287438.1. Transcript Variant: This variant (3) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094695.1, SRR1803611.206621.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TTGCTGTGCG TCATTCCCTA CTCAGGCCAA GCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274367.1
CDS	229..1368
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVYCCASFPTQAKRF

Target ORF information:

RefSeq Version	NM_001287438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287438.2

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TTGCTGTGCG TCATTCCCTA CTCAGGCCAA GCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001287438.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274367.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06114
Clone ID Related Accession (Same CDS sequence)	NM_001287438.2 , NM_001287438.3
Accession Version	NM_001287438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC111496.1, BC094695.1 and DA245843.1. On May 31, 2019 this sequence version replaced NM_001287438.2. Transcript Variant: This variant (3) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094695.1, SRR1803611.206621.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TTGCTGTGCG TCATTCCCTA CTCAGGCCAA GCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274367.1
CDS	177..1316
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVYCCASFPTQAKRF

Target ORF information:

RefSeq Version	NM_001287438.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287438.3

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TTGCTGTGCG TCATTCCCTA CTCAGGCCAA GCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346444.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333373.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu101284
Clone ID Related Accession (Same CDS sequence)	NM_001346444.1
Accession Version	NM_001346444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC111496.1 and AC004414.1. On Oct 13, 2016 this sequence version replaced XM_017011901.1. Transcript Variant: This variant (7) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (g) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111496.1, SRR1803613.215637.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TGGAGCAGCT GAAGCTGTGA TAAGACTTCT GAGCTTGCTT 
CTGAATACCA CAGCCCCGGG CACAGCAAAG CCAAGAACCC TGTGGATGAG TGAGGGCCGC 
AGTTCTCTAC ACAATCCTGA AATCAAGTGT TCCTGTTTCT CTTCCTCCTC CCCATTTCCC 
CAGTCACTGC TCAGCCTTCT CTTGGGTCTT TCAAGCATTT GTGAATGCAT GTGCACACTT 
AGACACACAC ACGCACACAC ACACAACCCA TCTTGTGTCT TACCTACCTC TGAAATATTG 
GCATTTGGGA GACAATCCCT AATCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333373.1
CDS	229..1638
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVYGAAEAVIRLLSLLLNTTAPGTAKPRTLWMSEGRSSLHNPEIKCSCFSSSSPFPQSLLSLLLGLSSICECMCTLRHTHAHTHNPSCVLPTSEILAFGRQSLIS

Target ORF information:

RefSeq Version	NM_001346444.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346444.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTTTA TGGAGCAGCT GAAGCTGTGA TAAGACTTCT GAGCTTGCTT 
CTGAATACCA CAGCCCCGGG CACAGCAAAG CCAAGAACCC TGTGGATGAG TGAGGGCCGC 
AGTTCTCTAC ACAATCCTGA AATCAAGTGT TCCTGTTTCT CTTCCTCCTC CCCATTTCCC 
CAGTCACTGC TCAGCCTTCT CTTGGGTCTT TCAAGCATTT GTGAATGCAT GTGCACACTT 
AGACACACAC ACGCACACAC ACACAACCCA TCTTGTGTCT TACCTACCTC TGAAATATTG 
GCATTTGGGA GACAATCCCT AATCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333370.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101282
Clone ID Related Accession (Same CDS sequence)	NM_001346441.2 , NM_001346441.1
Accession Version	NM_001346441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, AC012372.4, AC004414.1 and BC045771.1. On May 31, 2019 this sequence version replaced NM_001346441.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.30356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333370.1
CDS	177..3869
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346441.2

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346441.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333370.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101282
Clone ID Related Accession (Same CDS sequence)	NM_001346441.2 , NM_001346441.1
Accession Version	NM_001346441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, AC012372.4, AC004414.1 and BC045771.1. On Oct 13, 2016 this sequence version replaced XM_017011899.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.30356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333370.1
CDS	229..3921
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346441.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346441.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346443.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333372.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.

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CloneID	OHu101283
Clone ID Related Accession (Same CDS sequence)	NM_001346443.2 , NM_001346443.1
Accession Version	NM_001346443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, AC012372.4, AC004414.1 and BC045771.1. On May 31, 2019 this sequence version replaced NM_001346443.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (f) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.52106.1, SRR1803611.45429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333372.1
CDS	177..3698
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346443.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346443.2

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346443.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333372.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.

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CloneID	OHu101283
Clone ID Related Accession (Same CDS sequence)	NM_001346443.2 , NM_001346443.1
Accession Version	NM_001346443.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, AC012372.4, AC004414.1 and BC045771.1. On Oct 13, 2016 this sequence version replaced XM_017011900.1. Transcript Variant: This variant (6) lacks two alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (f) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.52106.1, SRR1803611.45429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333372.1
CDS	229..3750
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346443.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346443.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_017011898.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867387.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu97125
Clone ID Related Accession (Same CDS sequence)	XM_017011898.1
Accession Version	XM_017011898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGG CTGTTTAACG 
ACCCCCAACT CCCCATCCAT GCACTCACGT TCTCTTACGC TGGGTCCATC CCTCTCGCTG 
GGCAGCATCT CAGGGGTGTC CGTGAAGTCG GAGATGAAGA AGCGCCGAGC CCCTCCTCCT 
CCAGGTTCAG GGCCACCTGT GCAAGACAAG GCATCGGAAA AGGTATCTCT TGGGTCACAG 
ATTGATTTAC AGAAGAAGAA GCGGCGAGCG CCAGCTCCCC CTCCACCACA GCCACCACCA 
CCGAGTCCCC TGATCCCCAA CCGCACTGAG GATAAGGAGG AGAACAGGAA GAGCACGATG 
GTAAGCCTGC CCCTGGGGTC TGGCAGCCAC TGCAGCCCGG ATGGAGCCCC GCAGGTGCTG 
TCAGAGGCGG AGGAGACCGT GTCAGTTGGC AGCTGTTTTG CGTCGGAGGA CACGACCGAG 
GACTCAGGAG TGATGAGTTC CCCCTCAGAC ATCGTCTCTC TGGACTCGCA GCAGGACAGC 
ATGAAATACA AAGACAAGTG GGCCACAGAC CAGGAAGACT GCAGTGACCA GGACCTCGCT 
GGAACCCCAG ACCTGGGTCC CCAGAAGAGC CCCTTGTGGG AGAAGAATGG CTCTGAGAAC 
TCACATCTGA GAACTGAAAA AGCTGTCACA GCCTCAAATG ATGAAGAAGA CCTATTAATT 
GCTGGAGAGT TCCGTAAAAC CCTTGCAGAA CTTGATGAAG ACCTGGAAGA AATGGAAGAC 
AGCTATGAAA CAGACACCAG CTCCCTCACC AGCTCCATCC ATGGTGCATC CAACCACTGC 
CCACAGGATG CCATGATCCC TCACGGCGAC ACAGATGCAA TCCCAGTAAC ATTCATAGGG 
GAAGTTTCAG ATGATCCTGT GGATTCGGGG TTGTTTTCCA ATAGAAACAA CAATGCTGGG 
TCTTTCGACT CGGAGGGTGT TGCCAGCAGG AGAGACTCCC TGGCGCCACT TCAAGCTGAG 
CACAGCCAGC CCCATGAAAA GGCAAGGGAG GAAGTACCTG CCCTGCACCC CGCTTCCCAT 
GACGTCGGAA AAGGAATCCG TGTGGCCTTA TCTAACATCT CTAAAGATGG GAATCTAATG 
GAAACGGCGC CCAGGGTGAC TTCTTTTGCT TCGAATCTTC ACACAGACAA CCTAAATGCA 
AAAGTGAAAG ACAAAGTGTA TGGCTGTGCT GACGGGGAGA GGACTCAAGC CACAGAGAGA 
GTGAATTCCC AACCGGTGAA TGAAAAGGAC AGCAACGATA AAAACGCTGC CTTGGCACCA 
ACATCATGGC ACCAACGAGG CCAAAACCCA GGGAAAAGCT ACAGACTTAA GCACGGCCTC 
ACAACGTATA AAATCATTCC TCCAAAGTCA GAGATGCGAT GTTACGACAG AGATGTGTCC 
CTCTCCACCG GAGCCATTAA GATTGACGAG CTGGGGAACT TGGTGAGTCC TCACGCCACC 
GGCATCAGGA TCATTTCCCT GTCTTCGTCT GTGCCTGAAG CAGAATCTCA GCCCATTGGG 
AAAGTCAGAG AGTTCTGGAG GTGCAACTCT GTGGAAAAAC ACCTGGGCCG ACCCTCAGAG 
AGCTCTGCCA GGGGACCCCC CTCCACCCCT GTGCCCACGC AGACGCAGAA TCCAGAGAGC 
AGACTCCAAG CAGACCCCAA GCCAATATCG CCCCAGCAGA AGTCTGCCCA CCATGAGGGC 
CGGAACCCCC TAGGGGAGGG GAGAAACCAG CCCCCCACCA TGGGCATGGG TCACGTGAGG 
GTGCCAGCAG CTCACACCAC AGAAGTCACA TTTCTCAAGC CTCAGAGAAG AACGTCCAGC 
CAGTATGTGG CCTCTGCCAT TGCCAAGCGC ATAGGAGCCC CAAAAGTCCA TGCTGATGTG 
GTGAGGCCAC ACGGTTATGC CGAGAAGGGT TATGCAGGCA AGGCGCCAGT GCTGGCTGCA 
CCACCTGTCA CTGTGAAAGA TGACAGGACA TCCAGCCCAC ACTCAGAGAC ACAAGGATGG 
AAGGATGGTG CCCAGTGGCC CTGTGTCACT CCTCCCAACA ACCACGGGGA AGATTTGGCA 
GTGGGAGCCC CTCCTAGGGG GGAGGTCATT GGCCCACACA GGAAGTTGTC TACTCAGGAC 
AGACCTGCTG CTATCCACAG AAGCTCCTGT TTCTCACTGG TTCAGTCTTC CCAGAGGGAT 
CGTGTTTCTG TGGGACAGAG CTGTGGTTTC AGTGGAAAGC AAAGCACAAG TAGCCAGGAG 
GCCAGCTCAG CATCTGAGCC CAGACGCGCA CCTGATGGTA CAGACCCACC CCCTCCACAC 
ACATCTGACA CTCAGGCCTG CAGCAGGGAG CTGGTCAATG GCTCTGTGCG CGCCCCAGGC 
CACGGCGAGC CTTCCCACCC TCCAGGAGGG TCTGGCACAG AAAGCCACAT TCTCCTAGAA 
AGAGAGGAAA AGCCCAGTGT GTTCTCTACA GATGGAAATG AAACAGACAG TATTTGGCCA 
CCCAGCATTT TTGGGCCGAA GAAAAAATTC AAACCTGTTG TCCAGAGACC AGTCCCAAAA 
GACACATCCC TGCACTCTGC CCTGATGGAA GCCATCCACT CTGCGGGAGG GAAGGACAGA 
CTACGCAAGA CGGCAGAACA CACCGGGGAG GGCAGGCCAG CGAAACTGTC CTACACGGAG 
GCAGAGGGCG AACGATCTGC ACTGCTGGCA GCTATCCGCG GGCACAGCGG CACCTGCAGC 
CTGAGGAAGG TGGCATCCTC TGCTTCTGAG GAGCTCCAGA GCTTCCGAGA TGCCGCACTC 
TCTGCTCAGG GCTCGGAAAG TCCTCTGCTA GAAGACCTTG GTCTTCTGTC CCCACCAGCC 
ATTCCCCCAC CACCCCCTCC ACCCTCCCAG GCTCTCTCTG CACCAAGGAC GGCCTCCAGG 
TTCAGCACGG GCACCCTCAG CAACACCGCA GACGCAAGGC AAGCCTTGAT GGACGCCATC 
CGCTCCGGCA CAGGGGCTGC GAGACTGAGA AAGGTGCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867387.1
CDS	177..4007
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_017011898.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011898.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGG CTGTTTAACG 
ACCCCCAACT CCCCATCCAT GCACTCACGT TCTCTTACGC TGGGTCCATC CCTCTCGCTG 
GGCAGCATCT CAGGGGTGTC CGTGAAGTCG GAGATGAAGA AGCGCCGAGC CCCTCCTCCT 
CCAGGTTCAG GGCCACCTGT GCAAGACAAG GCATCGGAAA AGGTATCTCT TGGGTCACAG 
ATTGATTTAC AGAAGAAGAA GCGGCGAGCG CCAGCTCCCC CTCCACCACA GCCACCACCA 
CCGAGTCCCC TGATCCCCAA CCGCACTGAG GATAAGGAGG AGAACAGGAA GAGCACGATG 
GTAAGCCTGC CCCTGGGGTC TGGCAGCCAC TGCAGCCCGG ATGGAGCCCC GCAGGTGCTG 
TCAGAGGCGG AGGAGACCGT GTCAGTTGGC AGCTGTTTTG CGTCGGAGGA CACGACCGAG 
GACTCAGGAG TGATGAGTTC CCCCTCAGAC ATCGTCTCTC TGGACTCGCA GCAGGACAGC 
ATGAAATACA AAGACAAGTG GGCCACAGAC CAGGAAGACT GCAGTGACCA GGACCTCGCT 
GGAACCCCAG ACCTGGGTCC CCAGAAGAGC CCCTTGTGGG AGAAGAATGG CTCTGAGAAC 
TCACATCTGA GAACTGAAAA AGCTGTCACA GCCTCAAATG ATGAAGAAGA CCTATTAATT 
GCTGGAGAGT TCCGTAAAAC CCTTGCAGAA CTTGATGAAG ACCTGGAAGA AATGGAAGAC 
AGCTATGAAA CAGACACCAG CTCCCTCACC AGCTCCATCC ATGGTGCATC CAACCACTGC 
CCACAGGATG CCATGATCCC TCACGGCGAC ACAGATGCAA TCCCAGTAAC ATTCATAGGG 
GAAGTTTCAG ATGATCCTGT GGATTCGGGG TTGTTTTCCA ATAGAAACAA CAATGCTGGG 
TCTTTCGACT CGGAGGGTGT TGCCAGCAGG AGAGACTCCC TGGCGCCACT TCAAGCTGAG 
CACAGCCAGC CCCATGAAAA GGCAAGGGAG GAAGTACCTG CCCTGCACCC CGCTTCCCAT 
GACGTCGGAA AAGGAATCCG TGTGGCCTTA TCTAACATCT CTAAAGATGG GAATCTAATG 
GAAACGGCGC CCAGGGTGAC TTCTTTTGCT TCGAATCTTC ACACAGACAA CCTAAATGCA 
AAAGTGAAAG ACAAAGTGTA TGGCTGTGCT GACGGGGAGA GGACTCAAGC CACAGAGAGA 
GTGAATTCCC AACCGGTGAA TGAAAAGGAC AGCAACGATA AAAACGCTGC CTTGGCACCA 
ACATCATGGC ACCAACGAGG CCAAAACCCA GGGAAAAGCT ACAGACTTAA GCACGGCCTC 
ACAACGTATA AAATCATTCC TCCAAAGTCA GAGATGCGAT GTTACGACAG AGATGTGTCC 
CTCTCCACCG GAGCCATTAA GATTGACGAG CTGGGGAACT TGGTGAGTCC TCACGCCACC 
GGCATCAGGA TCATTTCCCT GTCTTCGTCT GTGCCTGAAG CAGAATCTCA GCCCATTGGG 
AAAGTCAGAG AGTTCTGGAG GTGCAACTCT GTGGAAAAAC ACCTGGGCCG ACCCTCAGAG 
AGCTCTGCCA GGGGACCCCC CTCCACCCCT GTGCCCACGC AGACGCAGAA TCCAGAGAGC 
AGACTCCAAG CAGACCCCAA GCCAATATCG CCCCAGCAGA AGTCTGCCCA CCATGAGGGC 
CGGAACCCCC TAGGGGAGGG GAGAAACCAG CCCCCCACCA TGGGCATGGG TCACGTGAGG 
GTGCCAGCAG CTCACACCAC AGAAGTCACA TTTCTCAAGC CTCAGAGAAG AACGTCCAGC 
CAGTATGTGG CCTCTGCCAT TGCCAAGCGC ATAGGAGCCC CAAAAGTCCA TGCTGATGTG 
GTGAGGCCAC ACGGTTATGC CGAGAAGGGT TATGCAGGCA AGGCGCCAGT GCTGGCTGCA 
CCACCTGTCA CTGTGAAAGA TGACAGGACA TCCAGCCCAC ACTCAGAGAC ACAAGGATGG 
AAGGATGGTG CCCAGTGGCC CTGTGTCACT CCTCCCAACA ACCACGGGGA AGATTTGGCA 
GTGGGAGCCC CTCCTAGGGG GGAGGTCATT GGCCCACACA GGAAGTTGTC TACTCAGGAC 
AGACCTGCTG CTATCCACAG AAGCTCCTGT TTCTCACTGG TTCAGTCTTC CCAGAGGGAT 
CGTGTTTCTG TGGGACAGAG CTGTGGTTTC AGTGGAAAGC AAAGCACAAG TAGCCAGGAG 
GCCAGCTCAG CATCTGAGCC CAGACGCGCA CCTGATGGTA CAGACCCACC CCCTCCACAC 
ACATCTGACA CTCAGGCCTG CAGCAGGGAG CTGGTCAATG GCTCTGTGCG CGCCCCAGGC 
CACGGCGAGC CTTCCCACCC TCCAGGAGGG TCTGGCACAG AAAGCCACAT TCTCCTAGAA 
AGAGAGGAAA AGCCCAGTGT GTTCTCTACA GATGGAAATG AAACAGACAG TATTTGGCCA 
CCCAGCATTT TTGGGCCGAA GAAAAAATTC AAACCTGTTG TCCAGAGACC AGTCCCAAAA 
GACACATCCC TGCACTCTGC CCTGATGGAA GCCATCCACT CTGCGGGAGG GAAGGACAGA 
CTACGCAAGA CGGCAGAACA CACCGGGGAG GGCAGGCCAG CGAAACTGTC CTACACGGAG 
GCAGAGGGCG AACGATCTGC ACTGCTGGCA GCTATCCGCG GGCACAGCGG CACCTGCAGC 
CTGAGGAAGG TGGCATCCTC TGCTTCTGAG GAGCTCCAGA GCTTCCGAGA TGCCGCACTC 
TCTGCTCAGG GCTCGGAAAG TCCTCTGCTA GAAGACCTTG GTCTTCTGTC CCCACCAGCC 
ATTCCCCCAC CACCCCCTCC ACCCTCCCAG GCTCTCTCTG CACCAAGGAC GGCCTCCAGG 
TTCAGCACGG GCACCCTCAG CAACACCGCA GACGCAAGGC AAGCCTTGAT GGACGCCATC 
CGCTCCGGCA CAGGGGCTGC GAGACTGAGA AAGGTGCCCT TGCTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001287436.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274365.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 1, mRNA.

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CloneID	OHu06113
Clone ID Related Accession (Same CDS sequence)	NM_001287436.2
Accession Version	NM_001287436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC111496.1, BC136441.1, DA245843.1, BC150263.1 and CR936689.1. This sequence is a reference standard in the RefSeqGene project. On Oct 13, 2016 this sequence version replaced NM_001287436.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGCTCT CTCTGCACCA AGGACGGCCT CCAGGTTCAG CACGGGCACC 
CTCAGCAACA CCGCAGACGC AAGGCAAGCC TTGATGGACG CCATCCGCTC CGGCACAGGG 
GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274365.1
CDS	229..4044
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_001287436.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287436.2

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGCTCT CTCTGCACCA AGGACGGCCT CCAGGTTCAG CACGGGCACC 
CTCAGCAACA CCGCAGACGC AAGGCAAGCC TTGATGGACG CCATCCGCTC CGGCACAGGG 
GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_015198.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056013.2 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06870
Clone ID Related Accession (Same CDS sequence)	NM_015198.5 , NM_015198.4
Accession Version	NM_015198.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC111496.1, AB014533.1, DA245843.1, BC045771.1 and AC004414.1. On Nov 23, 2018 this sequence version replaced NM_015198.4. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150263.1, AB014533.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265136.12/ ENSP00000265136.7 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056013.2
CDS	177..3962
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_015198.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015198.5

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_015198.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056013.2 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06870
Clone ID Related Accession (Same CDS sequence)	NM_015198.5 , NM_015198.4
Accession Version	NM_015198.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC111496.1, AB014533.1, DA245843.1, BC045771.1 and AC004414.1. On Oct 13, 2016 this sequence version replaced NM_015198.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150263.1, AB014533.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056013.2
CDS	229..4014
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_015198.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015198.4

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346442.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333371.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.

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CloneID	OHu41131
Clone ID Related Accession (Same CDS sequence)	NM_001346442.2 , NM_001346442.1
Accession Version	NM_001346442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC144099.1, AC004414.1 and BC045771.1. On May 31, 2019 this sequence version replaced NM_001346442.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (e) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.189413.1, SRR1803615.139158.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333371.1
CDS	177..3821
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346442.2

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( NM_001346442.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333371.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.

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CloneID	OHu41131
Clone ID Related Accession (Same CDS sequence)	NM_001346442.2 , NM_001346442.1
Accession Version	NM_001346442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005535.2, BC144099.1, AC004414.1 and BC045771.1. On Oct 13, 2016 this sequence version replaced XM_005271756.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (e) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.189413.1, SRR1660807.104534.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333371.1
CDS	229..3873
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	NM_001346442.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346442.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_005271751.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271808.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu41126
Clone ID Related Accession (Same CDS sequence)	XM_005271751.1
Accession Version	XM_005271751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGGC ATCCTCTGCT TCTGAGGAGC TCCAGAGCTT CCGAGATGCC 
GCACTCTCTG CTCAGGGCTC GGAAAGTCCT CTGCTAGAAG ACCTTGGTCT TCTGTCCCCA 
CCAGCCATTC CCCCACCACC CCCTCCACCC TCCCAGGCTC TCTCTGCACC AAGGACGGCC 
TCCAGGTTCA GCACGGGCAC CCTCAGCAAC ACCGCAGACG CAAGGCAAGC CTTGATGGAC 
GCCATCCGCT CCGGCACAGG GGCTGCGAGA CTGAGAAAGG TGCCCTTGCT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271808.1
CDS	177..4133
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_005271751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271751.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGGC ATCCTCTGCT TCTGAGGAGC TCCAGAGCTT CCGAGATGCC 
GCACTCTCTG CTCAGGGCTC GGAAAGTCCT CTGCTAGAAG ACCTTGGTCT TCTGTCCCCA 
CCAGCCATTC CCCCACCACC CCCTCCACCC TCCCAGGCTC TCTCTGCACC AAGGACGGCC 
TCCAGGTTCA GCACGGGCAC CCTCAGCAAC ACCGCAGACG CAAGGCAAGC CTTGATGGAC 
GCCATCCGCT CCGGCACAGG GGCTGCGAGA CTGAGAAAGG TGCCCTTGCT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515241.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513543.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu41132
Clone ID Related Accession (Same CDS sequence)	XM_011515241.1
Accession Version	XM_011515241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCAC GTTCTCTTAC GCTGGGTCCA TCCCTCTCGC TGGGCAGCAT CTCAGGGGTG 
TCCGTGAAGT CGGAGATGAA GAAGCGCCGA GCCCCTCCTC CTCCAGGTTC AGGGCCACCT 
GTGCAAGACA AGGCATCGGA AAAGGTATCT CTTGGGTCAC AGATTGATTT ACAGAAGAAG 
AAGCGGCGAG CGCCAGCTCC CCCTCCACCA CAGCCACCAC CACCGAGTCC CCTGATCCCC 
AACCGCACTG AGGATAAGGA GGAGAACAGG AAGAGCACGA TGGGTGGTGG ACGGCAGGTG 
CCACAAAAGC CTCCCAGAGG CACTGCTCGG GGTCCACCCC AGTTAGTGCT TCCCCCACCC 
CCGCCCTACC CTCCTCCCGA CACAGACGTG GTGGAGCCTC TCAGCTTTCC CGGGGAAGGC 
GCTGGTTCCG AGGCCTCAGA CCCGATACCC AAACTAAGCC TGCCCCTGGG GTCTGGCAGC 
CACTGCAGCC CGGATGGAGC CCCGCAGGTG CTGTCAGAGG CGGAGGAGAC CGTGTCAGTT 
GGCAGCTGTT TTGCGTCGGA GGACACGACC GAGGACTCAG GAGTGATGAG TTCCCCCTCA 
GACATCGTCT CTCTGGACTC GCAGCAGGAC AGCATGAAAT ACAAAGACAA GTGGGCCACA 
GACCAGGAAG ACTGCAGTGA CCAGGACCTC GCTGGAACCC CAGACCTGGG TCCCCAGAAG 
AGCCCCTTGT GGGAGAAGAA TGGCTCTGAG AACTCACATC TGAGAACTGA AAAAGCTGTC 
ACAGCCTCAA ATGATGAAGA AGACCTATTA ATTGCTGGAG AGTTCCGTAA AACCCTTGCA 
GAACTTGATG AAGACCTGGA AGAAATGGAA GACAGCTATG AAACAGACAC CAGCTCCCTC 
ACCAGCTCCA TCCATGGTGC ATCCAACCAC TGCCCACAGG ATGCCATGAT CCCTCACGGC 
GACACAGATG CAATCCCAGT AACATTCATA GGGGAAGTTT CAGATGATCC TGTGGATTCG 
GGGTTGTTTT CCAATAGAAA CAACAATGCT GGGTCTTTCG ACTCGGAGGG TGTTGCCAGC 
AGGAGAGACT CCCTGGCGCC ACTTCAAGCT GAGCACAGCC AGCCCCATGA AAAGGCAAGG 
GAGGAAGTAC CTGCCCTGCA CCCCGCTTCC CATGACGTCG GAAAAGGAAT CCGTGTGGCC 
TTATCTAACA TCTCTAAAGA TGGGAATCTA ATGGAAACGG CGCCCAGGGT GACTTCTTTT 
GCTTCGAATC TTCACACAGA CAACCTAAAT GCAAAAGTGA AAGACAAAGT GTATGGCTGT 
GCTGACGGGG AGAGGACTCA AGCCACAGAG AGAGTGAATT CCCAACCGGT GAATGAAAAG 
GACAGCAACG ATAAAAACGC TGCCTTGGCA CCAACATCAT GGCACCAACG AGGCCAAAAC 
CCAGGGAAAA GCTACAGACT TAAGCACGGC CTCACAACGT ATAAAATCAT TCCTCCAAAG 
TCAGAGATGC GATGTTACGA CAGAGATGTG TCCCTCTCCA CCGGAGCCAT TAAGATTGAC 
GAGCTGGGGA ACTTGGTGAG TCCTCACGCC ACCGGCATCA GGATCATTTC CCTGTCTTCG 
TCTGTGCCTG AAGCAGAATC TCAGCCCATT GGGAAAGTCA GAGAGTTCTG GAGGTGCAAC 
TCTGTGGAAA AACACCTGGG CCGACCCTCA GAGAGCTCTG CCAGGGGACC CCCCTCCACC 
CCTGTGCCCA CGCAGACGCA GAATCCAGAG AGCAGACTCC AAGCAGACCC CAAGCCAATA 
TCGCCCCAGC AGAAGTCTGC CCACCATGAG GGCCGGAACC CCCTAGGGGA GGGGAGAAAC 
CAGCCCCCCA CCATGGGCAT GGGTCACGTG AGGGTGCCAG CAGCTCACAC CACAGAAGTC 
ACATTTCTCA AGCCTCAGAG AAGAACGTCC AGCCAGTATG TGGCCTCTGC CATTGCCAAG 
CGCATAGGAG CCCCAAAAGT CCATGCTGAT GTGGTGAGGC CACACGGTTA TGCCGAGAAG 
GGTTATGCAG GCAAGGCGCC AGTGCTGGCT GCACCACCTG TCACTGTGAA AGATGACAGG 
ACATCCAGCC CACACTCAGA GACACAAGGA TGGAAGGATG GTGCCCAGTG GCCCTGTGTC 
ACTCCTCCCA ACAACCACGG GGAAGATTTG GCAGTGGGAG CCCCTCCTAG GGGGGAGGTC 
ATTGGCCCAC ACAGGAAGTT GTCTACTCAG GACAGACCTG CTGCTATCCA CAGAAGCTCC 
TGTTTCTCAC TGGTTCAGTC TTCCCAGAGG GATCGTGTTT CTGTGGGACA GAGCTGTGGT 
TTCAGTGGAA AGCAAAGCAC AAGTAGCCAG GAGGCCAGCT CAGCATCTGA GCCCAGACGC 
GCACCTGATG GTACAGACCC ACCCCCTCCA CACACATCTG ACACTCAGGC CTGCAGCAGG 
GAGCTGGTCA ATGGCTCTGT GCGCGCCCCA GGCCACGGCG AGCCTTCCCA CCCTCCAGGA 
GGGTCTGGCA CAGAAAGCCA CATTCTCCTA GAAAGAGAGG AAAAGCCCAG TGTGTTCTCT 
ACAGATGGAA ATGAAACAGA CAGTATTTGG CCACCCAGCA TTTTTGGGCC GAAGAAAAAA 
TTCAAACCTG TTGTCCAGAG ACCAGTCCCA AAAGACACAT CCCTGCACTC TGCCCTGATG 
GAAGCCATCC ACTCTGCGGG AGGGAAGGAC AGACTACGCA AGACGGCAGA ACACACCGGG 
GAGGGCAGGC CAGCGAAACT GTCCTACACG GAGGCAGAGG GCGAACGATC TGCACTGCTG 
GCAGCTATCC GCGGGCACAG CGGCACCTGC AGCCTGAGGA AGGTGGCATC CTCTGCTTCT 
GAGGAGCTCC AGAGCTTCCG AGATGCCGCA CTCTCTGCTC AGGGCTCGGA AAGTCCTCTG 
CTAGAAGACC TTGGTCTTCT GTCCCCACCA GCCATTCCCC CACCACCCCC TCCACCCTCC 
CAGGCTCTCT CTGCACCAAG GACGGCCTCC AGGTTCAGCA CGGGCACCCT CAGCAACACC 
GCAGACGCAA GGCAAGCCTT GATGGACGCC ATCCGCTCCG GCACAGGGGC TGCGAGACTG 
AGAAAGGTGC CCTTGCTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513543.1
CDS	114..3257
Translation	MHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515241.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515241.1

ORF Insert Sequence:

ATGCACTCAC GTTCTCTTAC GCTGGGTCCA TCCCTCTCGC TGGGCAGCAT CTCAGGGGTG 
TCCGTGAAGT CGGAGATGAA GAAGCGCCGA GCCCCTCCTC CTCCAGGTTC AGGGCCACCT 
GTGCAAGACA AGGCATCGGA AAAGGTATCT CTTGGGTCAC AGATTGATTT ACAGAAGAAG 
AAGCGGCGAG CGCCAGCTCC CCCTCCACCA CAGCCACCAC CACCGAGTCC CCTGATCCCC 
AACCGCACTG AGGATAAGGA GGAGAACAGG AAGAGCACGA TGGGTGGTGG ACGGCAGGTG 
CCACAAAAGC CTCCCAGAGG CACTGCTCGG GGTCCACCCC AGTTAGTGCT TCCCCCACCC 
CCGCCCTACC CTCCTCCCGA CACAGACGTG GTGGAGCCTC TCAGCTTTCC CGGGGAAGGC 
GCTGGTTCCG AGGCCTCAGA CCCGATACCC AAACTAAGCC TGCCCCTGGG GTCTGGCAGC 
CACTGCAGCC CGGATGGAGC CCCGCAGGTG CTGTCAGAGG CGGAGGAGAC CGTGTCAGTT 
GGCAGCTGTT TTGCGTCGGA GGACACGACC GAGGACTCAG GAGTGATGAG TTCCCCCTCA 
GACATCGTCT CTCTGGACTC GCAGCAGGAC AGCATGAAAT ACAAAGACAA GTGGGCCACA 
GACCAGGAAG ACTGCAGTGA CCAGGACCTC GCTGGAACCC CAGACCTGGG TCCCCAGAAG 
AGCCCCTTGT GGGAGAAGAA TGGCTCTGAG AACTCACATC TGAGAACTGA AAAAGCTGTC 
ACAGCCTCAA ATGATGAAGA AGACCTATTA ATTGCTGGAG AGTTCCGTAA AACCCTTGCA 
GAACTTGATG AAGACCTGGA AGAAATGGAA GACAGCTATG AAACAGACAC CAGCTCCCTC 
ACCAGCTCCA TCCATGGTGC ATCCAACCAC TGCCCACAGG ATGCCATGAT CCCTCACGGC 
GACACAGATG CAATCCCAGT AACATTCATA GGGGAAGTTT CAGATGATCC TGTGGATTCG 
GGGTTGTTTT CCAATAGAAA CAACAATGCT GGGTCTTTCG ACTCGGAGGG TGTTGCCAGC 
AGGAGAGACT CCCTGGCGCC ACTTCAAGCT GAGCACAGCC AGCCCCATGA AAAGGCAAGG 
GAGGAAGTAC CTGCCCTGCA CCCCGCTTCC CATGACGTCG GAAAAGGAAT CCGTGTGGCC 
TTATCTAACA TCTCTAAAGA TGGGAATCTA ATGGAAACGG CGCCCAGGGT GACTTCTTTT 
GCTTCGAATC TTCACACAGA CAACCTAAAT GCAAAAGTGA AAGACAAAGT GTATGGCTGT 
GCTGACGGGG AGAGGACTCA AGCCACAGAG AGAGTGAATT CCCAACCGGT GAATGAAAAG 
GACAGCAACG ATAAAAACGC TGCCTTGGCA CCAACATCAT GGCACCAACG AGGCCAAAAC 
CCAGGGAAAA GCTACAGACT TAAGCACGGC CTCACAACGT ATAAAATCAT TCCTCCAAAG 
TCAGAGATGC GATGTTACGA CAGAGATGTG TCCCTCTCCA CCGGAGCCAT TAAGATTGAC 
GAGCTGGGGA ACTTGGTGAG TCCTCACGCC ACCGGCATCA GGATCATTTC CCTGTCTTCG 
TCTGTGCCTG AAGCAGAATC TCAGCCCATT GGGAAAGTCA GAGAGTTCTG GAGGTGCAAC 
TCTGTGGAAA AACACCTGGG CCGACCCTCA GAGAGCTCTG CCAGGGGACC CCCCTCCACC 
CCTGTGCCCA CGCAGACGCA GAATCCAGAG AGCAGACTCC AAGCAGACCC CAAGCCAATA 
TCGCCCCAGC AGAAGTCTGC CCACCATGAG GGCCGGAACC CCCTAGGGGA GGGGAGAAAC 
CAGCCCCCCA CCATGGGCAT GGGTCACGTG AGGGTGCCAG CAGCTCACAC CACAGAAGTC 
ACATTTCTCA AGCCTCAGAG AAGAACGTCC AGCCAGTATG TGGCCTCTGC CATTGCCAAG 
CGCATAGGAG CCCCAAAAGT CCATGCTGAT GTGGTGAGGC CACACGGTTA TGCCGAGAAG 
GGTTATGCAG GCAAGGCGCC AGTGCTGGCT GCACCACCTG TCACTGTGAA AGATGACAGG 
ACATCCAGCC CACACTCAGA GACACAAGGA TGGAAGGATG GTGCCCAGTG GCCCTGTGTC 
ACTCCTCCCA ACAACCACGG GGAAGATTTG GCAGTGGGAG CCCCTCCTAG GGGGGAGGTC 
ATTGGCCCAC ACAGGAAGTT GTCTACTCAG GACAGACCTG CTGCTATCCA CAGAAGCTCC 
TGTTTCTCAC TGGTTCAGTC TTCCCAGAGG GATCGTGTTT CTGTGGGACA GAGCTGTGGT 
TTCAGTGGAA AGCAAAGCAC AAGTAGCCAG GAGGCCAGCT CAGCATCTGA GCCCAGACGC 
GCACCTGATG GTACAGACCC ACCCCCTCCA CACACATCTG ACACTCAGGC CTGCAGCAGG 
GAGCTGGTCA ATGGCTCTGT GCGCGCCCCA GGCCACGGCG AGCCTTCCCA CCCTCCAGGA 
GGGTCTGGCA CAGAAAGCCA CATTCTCCTA GAAAGAGAGG AAAAGCCCAG TGTGTTCTCT 
ACAGATGGAA ATGAAACAGA CAGTATTTGG CCACCCAGCA TTTTTGGGCC GAAGAAAAAA 
TTCAAACCTG TTGTCCAGAG ACCAGTCCCA AAAGACACAT CCCTGCACTC TGCCCTGATG 
GAAGCCATCC ACTCTGCGGG AGGGAAGGAC AGACTACGCA AGACGGCAGA ACACACCGGG 
GAGGGCAGGC CAGCGAAACT GTCCTACACG GAGGCAGAGG GCGAACGATC TGCACTGCTG 
GCAGCTATCC GCGGGCACAG CGGCACCTGC AGCCTGAGGA AGGTGGCATC CTCTGCTTCT 
GAGGAGCTCC AGAGCTTCCG AGATGCCGCA CTCTCTGCTC AGGGCTCGGA AAGTCCTCTG 
CTAGAAGACC TTGGTCTTCT GTCCCCACCA GCCATTCCCC CACCACCCCC TCCACCCTCC 
CAGGCTCTCT CTGCACCAAG GACGGCCTCC AGGTTCAGCA CGGGCACCCT CAGCAACACC 
GCAGACGCAA GGCAAGCCTT GATGGACGCC ATCCGCTCCG GCACAGGGGC TGCGAGACTG 
AGAAAGGTGC CCTTGCTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513538.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu65779
Clone ID Related Accession (Same CDS sequence)	XM_011515236.1
Accession Version	XM_011515236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGCTCT CTCTGCACCA AGGACGGCCT CCAGGTTCAG CACGGGCACC 
CTCAGCAACA CCGCAGACGC AAGGCAAGCC TTGATGGACG CCATCCGCTC CGGCACAGGG 
GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513538.1
CDS	177..4112
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515236.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGCTCT CTCTGCACCA AGGACGGCCT CCAGGTTCAG CACGGGCACC 
CTCAGCAACA CCGCAGACGC AAGGCAAGCC TTGATGGACG CCATCCGCTC CGGCACAGGG 
GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515237.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513539.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA.

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CloneID	OHu65780
Clone ID Related Accession (Same CDS sequence)	XM_011515237.1
Accession Version	XM_011515237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513539.1
CDS	177..4082
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515237.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515237.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGTGGCA TCCTCTGCTT CTGAGGAGCT CCAGAGCTTC 
CGAGATGCCG CACTCTCTGC TCAGGGCTCG GAAAGTCCTC TGCTAGAAGA CCTTGGTCTT 
CTGTCCCCAC CAGCCATTCC CCCACCACCC CCTCCACCCT CCCAGGCTCT CTCTGCACCA 
AGGACGGCCT CCAGGTTCAG CACGGGCACC CTCAGCAACA CCGCAGACGC AAGGCAAGCC 
TTGATGGACG CCATCCGCTC CGGCACAGGG GCTGCGAGAC TGAGAAAGGT GCCCTTGCTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515238.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513540.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA.

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CloneID	OHu65781
Clone ID Related Accession (Same CDS sequence)	XM_011515238.1
Accession Version	XM_011515238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513540.1
CDS	177..3989
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515238.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGCC CTTGCTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513541.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu65782
Clone ID Related Accession (Same CDS sequence)	XM_011515239.1
Accession Version	XM_011515239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513541.1
CDS	177..3941
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515239.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGTAAG CCTGCCCCTG GGGTCTGGCA GCCACTGCAG CCCGGATGGA 
GCCCCGCAGG TGCTGTCAGA GGCGGAGGAG ACCGTGTCAG TTGGCAGCTG TTTTGCGTCG 
GAGGACACGA CCGAGGACTC AGGAGTGATG AGTTCCCCCT CAGACATCGT CTCTCTGGAC 
TCGCAGCAGG ACAGCATGAA ATACAAAGAC AAGTGGGCCA CAGACCAGGA AGACTGCAGT 
GACCAGGACC TCGCTGGAAC CCCAGACCTG GGTCCCCAGA AGAGCCCCTT GTGGGAGAAG 
AATGGCTCTG AGAACTCACA TCTGAGAACT GAAAAAGCTG TCACAGCCTC AAATGATGAA 
GAAGACCTAT TAATTGCTGG AGAGTTCCGT AAAACCCTTG CAGAACTTGA TGAAGACCTG 
GAAGAAATGG AAGACAGCTA TGAAACAGAC ACCAGCTCCC TCACCAGCTC CATCCATGGT 
GCATCCAACC ACTGCCCACA GGATGCCATG ATCCCTCACG GCGACACAGA TGCAATCCCA 
GTAACATTCA TAGGGGAAGT TTCAGATGAT CCTGTGGATT CGGGGTTGTT TTCCAATAGA 
AACAACAATG CTGGGTCTTT CGACTCGGAG GGTGTTGCCA GCAGGAGAGA CTCCCTGGCG 
CCACTTCAAG CTGAGCACAG CCAGCCCCAT GAAAAGGCAA GGGAGGAAGT ACCTGCCCTG 
CACCCCGCTT CCCATGACGT CGGAAAAGGA ATCCGTGTGG CCTTATCTAA CATCTCTAAA 
GATGGGAATC TAATGGAAAC GGCGCCCAGG GTGACTTCTT TTGCTTCGAA TCTTCACACA 
GACAACCTAA ATGCAAAAGT GAAAGACAAA GTGTATGGCT GTGCTGACGG GGAGAGGACT 
CAAGCCACAG AGAGAGTGAA TTCCCAACCG GTGAATGAAA AGGACAGCAA CGATAAAAAC 
GCTGCCTTGG CACCAACATC ATGGCACCAA CGAGGCCAAA ACCCAGGGAA AAGCTACAGA 
CTTAAGCACG GCCTCACAAC GTATAAAATC ATTCCTCCAA AGTCAGAGAT GCGATGTTAC 
GACAGAGATG TGTCCCTCTC CACCGGAGCC ATTAAGATTG ACGAGCTGGG GAACTTGGTG 
AGTCCTCACG CCACCGGCAT CAGGATCATT TCCCTGTCTT CGTCTGTGCC TGAAGCAGAA 
TCTCAGCCCA TTGGGAAAGT CAGAGAGTTC TGGAGGTGCA ACTCTGTGGA AAAACACCTG 
GGCCGACCCT CAGAGAGCTC TGCCAGGGGA CCCCCCTCCA CCCCTGTGCC CACGCAGACG 
CAGAATCCAG AGAGCAGACT CCAAGCAGAC CCCAAGCCAA TATCGCCCCA GCAGAAGTCT 
GCCCACCATG AGGGCCGGAA CCCCCTAGGG GAGGGGAGAA ACCAGCCCCC CACCATGGGC 
ATGGGTCACG TGAGGGTGCC AGCAGCTCAC ACCACAGAAG TCACATTTCT CAAGCCTCAG 
AGAAGAACGT CCAGCCAGTA TGTGGCCTCT GCCATTGCCA AGCGCATAGG AGCCCCAAAA 
GTCCATGCTG ATGTGGTGAG GCCACACGGT TATGCCGAGA AGGGTTATGC AGGCAAGGCG 
CCAGTGCTGG CTGCACCACC TGTCACTGTG AAAGATGACA GGACATCCAG CCCACACTCA 
GAGACACAAG GATGGAAGGA TGGTGCCCAG TGGCCCTGTG TCACTCCTCC CAACAACCAC 
GGGGAAGATT TGGCAGTGGG AGCCCCTCCT AGGGGGGAGG TCATTGGCCC ACACAGGAAG 
TTGTCTACTC AGGACAGACC TGCTGCTATC CACAGAAGCT CCTGTTTCTC ACTGGTTCAG 
TCTTCCCAGA GGGATCGTGT TTCTGTGGGA CAGAGCTGTG GTTTCAGTGG AAAGCAAAGC 
ACAAGTAGCC AGGAGGCCAG CTCAGCATCT GAGCCCAGAC GCGCACCTGA TGGTACAGAC 
CCACCCCCTC CACACACATC TGACACTCAG GCCTGCAGCA GGGAGCTGGT CAATGGCTCT 
GTGCGCGCCC CAGGCCACGG CGAGCCTTCC CACCCTCCAG GAGGGTCTGG CACAGAAAGC 
CACATTCTCC TAGAAAGAGA GGAAAAGCCC AGTGTGTTCT CTACAGATGG AAATGAAACA 
GACAGTATTT GGCCACCCAG CATTTTTGGG CCGAAGAAAA AATTCAAACC TGTTGTCCAG 
AGACCAGTCC CAAAAGACAC ATCCCTGCAC TCTGCCCTGA TGGAAGCCAT CCACTCTGCG 
GGAGGGAAGG ACAGACTACG CAAGACGGCA GAACACACCG GGGAGGGCAG GCCAGCGAAA 
CTGTCCTACA CGGAGGCAGA GGGCGAACGA TCTGCACTGC TGGCAGCTAT CCGCGGGCAC 
AGCGGCACCT GCAGCCTGAG GAAGGCTCTC TCTGCACCAA GGACGGCCTC CAGGTTCAGC 
ACGGGCACCC TCAGCAACAC CGCAGACGCA AGGCAAGCCT TGATGGACGC CATCCGCTCC 
GGCACAGGGG CTGCGAGACT GAGAAAGGTG CCCTTGCTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515240.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513542.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu65783
Clone ID Related Accession (Same CDS sequence)	XM_011515240.1
Accession Version	XM_011515240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGCTGAGC AGCTCGTGCT GTCGGGTGCA GACAGCGATG AGGACACCTC CAGGGCTGCC 
CCAGGAAGGG GTTTGAACGG CTGTTTAACG ACCCCCAACT CCCCATCCAT GCACTCACGT 
TCTCTTACGC TGGGTCCATC CCTCTCGCTG GGCAGCATCT CAGGGGTGTC CGTGAAGTCG 
GAGATGAAGA AGCGCCGAGC CCCTCCTCCT CCAGGTTCAG GGCCACCTGT GCAAGACAAG 
GCATCGGAAA AGGTATCTCT TGGGTCACAG ATTGATTTAC AGAAGAAGAA GCGGCGAGCG 
CCAGCTCCCC CTCCACCACA GCCACCACCA CCGAGTCCCC TGATCCCCAA CCGCACTGAG 
GATAAGGAGG AGAACAGGAA GAGCACGATG GTAAGCCTGC CCCTGGGGTC TGGCAGCCAC 
TGCAGCCCGG ATGGAGCCCC GCAGGTGCTG TCAGAGGCGG AGGAGACCGT GTCAGTTGGC 
AGCTGTTTTG CGTCGGAGGA CACGACCGAG GACTCAGGAG TGATGAGTTC CCCCTCAGAC 
ATCGTCTCTC TGGACTCGCA GCAGGACAGC ATGAAATACA AAGACAAGTG GGCCACAGAC 
CAGGAAGACT GCAGTGACCA GGACCTCGCT GGAACCCCAG ACCTGGGTCC CCAGAAGAGC 
CCCTTGTGGG AGAAGAATGG CTCTGAGAAC TCACATCTGA GAACTGAAAA AGCTGTCACA 
GCCTCAAATG ATGAAGAAGA CCTATTAATT GCTGGAGAGT TCCGTAAAAC CCTTGCAGAA 
CTTGATGAAG ACCTGGAAGA AATGGAAGAC AGCTATGAAA CAGACACCAG CTCCCTCACC 
AGCTCCATCC ATGGTGCATC CAACCACTGC CCACAGGATG CCATGATCCC TCACGGCGAC 
ACAGATGCAA TCCCAGTAAC ATTCATAGGG GAAGTTTCAG ATGATCCTGT GGATTCGGGG 
TTGTTTTCCA ATAGAAACAA CAATGCTGGG TCTTTCGACT CGGAGGGTGT TGCCAGCAGG 
AGAGACTCCC TGGCGCCACT TCAAGCTGAG CACAGCCAGC CCCATGAAAA GGCAAGGGAG 
GAAGTACCTG CCCTGCACCC CGCTTCCCAT GACGTCGGAA AAGGAATCCG TGTGGCCTTA 
TCTAACATCT CTAAAGATGG GAATCTAATG GAAACGGCGC CCAGGGTGAC TTCTTTTGCT 
TCGAATCTTC ACACAGACAA CCTAAATGCA AAAGTGAAAG ACAAAGTGTA TGGCTGTGCT 
GACGGGGAGA GGACTCAAGC CACAGAGAGA GTGAATTCCC AACCGGTGAA TGAAAAGGAC 
AGCAACGATA AAAACGCTGC CTTGGCACCA ACATCATGGC ACCAACGAGG CCAAAACCCA 
GGGAAAAGCT ACAGACTTAA GCACGGCCTC ACAACGTATA AAATCATTCC TCCAAAGTCA 
GAGATGCGAT GTTACGACAG AGATGTGTCC CTCTCCACCG GAGCCATTAA GATTGACGAG 
CTGGGGAACT TGGTGAGTCC TCACGCCACC GGCATCAGGA TCATTTCCCT GTCTTCGTCT 
GTGCCTGAAG CAGAATCTCA GCCCATTGGG AAAGTCAGAG AGTTCTGGAG GTGCAACTCT 
GTGGAAAAAC ACCTGGGCCG ACCCTCAGAG AGCTCTGCCA GGGGACCCCC CTCCACCCCT 
GTGCCCACGC AGACGCAGAA TCCAGAGAGC AGACTCCAAG CAGACCCCAA GCCAATATCG 
CCCCAGCAGA AGTCTGCCCA CCATGAGGGC CGGAACCCCC TAGGGGAGGG GAGAAACCAG 
CCCCCCACCA TGGGCATGGG TCACGTGAGG GTGCCAGCAG CTCACACCAC AGAAGTCACA 
TTTCTCAAGC CTCAGAGAAG AACGTCCAGC CAGTATGTGG CCTCTGCCAT TGCCAAGCGC 
ATAGGAGCCC CAAAAGTCCA TGCTGATGTG GTGAGGCCAC ACGGTTATGC CGAGAAGGGT 
TATGCAGGCA AGGCGCCAGT GCTGGCTGCA CCACCTGTCA CTGTGAAAGA TGACAGGACA 
TCCAGCCCAC ACTCAGAGAC ACAAGGATGG AAGGATGGTG CCCAGTGGCC CTGTGTCACT 
CCTCCCAACA ACCACGGGGA AGATTTGGCA GTGGGAGCCC CTCCTAGGGG GGAGGTCATT 
GGCCCACACA GGAAGTTGTC TACTCAGGAC AGACCTGCTG CTATCCACAG AAGCTCCTGT 
TTCTCACTGG TTCAGTCTTC CCAGAGGGAT CGTGTTTCTG TGGGACAGAG CTGTGGTTTC 
AGTGGAAAGC AAAGCACAAG TAGCCAGGAG GCCAGCTCAG CATCTGAGCC CAGACGCGCA 
CCTGATGGTA CAGACCCACC CCCTCCACAC ACATCTGACA CTCAGGCCTG CAGCAGGGAG 
CTGGTCAATG GCTCTGTGCG CGCCCCAGGC CACGGCGAGC CTTCCCACCC TCCAGGAGGG 
TCTGGCACAG AAAGCCACAT TCTCCTAGAA AGAGAGGAAA AGCCCAGTGT GTTCTCTACA 
GATGGAAATG AAACAGACAG TATTTGGCCA CCCAGCATTT TTGGGCCGAA GAAAAAATTC 
AAACCTGTTG TCCAGAGACC AGTCCCAAAA GACACATCCC TGCACTCTGC CCTGATGGAA 
GCCATCCACT CTGCGGGAGG GAAGGACAGA CTACGCAAGA CGGCAGAACA CACCGGGGAG 
GGCAGGCCAG CGAAACTGTC CTACACGGAG GCAGAGGGCG AACGATCTGC ACTGCTGGCA 
GCTATCCGCG GGCACAGCGG CACCTGCAGC CTGAGGAAGG CTCTCTCTGC ACCAAGGACG 
GCCTCCAGGT TCAGCACGGG CACCCTCAGC AACACCGCAG ACGCAAGGCA AGCCTTGATG 
GACGCCATCC GCTCCGGCAC AGGGGCTGCG AGACTGAGAA AGGTGCCCTT GCTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513542.1
CDS	177..3896
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMVSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515240.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGCTGAGC AGCTCGTGCT GTCGGGTGCA GACAGCGATG AGGACACCTC CAGGGCTGCC 
CCAGGAAGGG GTTTGAACGG CTGTTTAACG ACCCCCAACT CCCCATCCAT GCACTCACGT 
TCTCTTACGC TGGGTCCATC CCTCTCGCTG GGCAGCATCT CAGGGGTGTC CGTGAAGTCG 
GAGATGAAGA AGCGCCGAGC CCCTCCTCCT CCAGGTTCAG GGCCACCTGT GCAAGACAAG 
GCATCGGAAA AGGTATCTCT TGGGTCACAG ATTGATTTAC AGAAGAAGAA GCGGCGAGCG 
CCAGCTCCCC CTCCACCACA GCCACCACCA CCGAGTCCCC TGATCCCCAA CCGCACTGAG 
GATAAGGAGG AGAACAGGAA GAGCACGATG GTAAGCCTGC CCCTGGGGTC TGGCAGCCAC 
TGCAGCCCGG ATGGAGCCCC GCAGGTGCTG TCAGAGGCGG AGGAGACCGT GTCAGTTGGC 
AGCTGTTTTG CGTCGGAGGA CACGACCGAG GACTCAGGAG TGATGAGTTC CCCCTCAGAC 
ATCGTCTCTC TGGACTCGCA GCAGGACAGC ATGAAATACA AAGACAAGTG GGCCACAGAC 
CAGGAAGACT GCAGTGACCA GGACCTCGCT GGAACCCCAG ACCTGGGTCC CCAGAAGAGC 
CCCTTGTGGG AGAAGAATGG CTCTGAGAAC TCACATCTGA GAACTGAAAA AGCTGTCACA 
GCCTCAAATG ATGAAGAAGA CCTATTAATT GCTGGAGAGT TCCGTAAAAC CCTTGCAGAA 
CTTGATGAAG ACCTGGAAGA AATGGAAGAC AGCTATGAAA CAGACACCAG CTCCCTCACC 
AGCTCCATCC ATGGTGCATC CAACCACTGC CCACAGGATG CCATGATCCC TCACGGCGAC 
ACAGATGCAA TCCCAGTAAC ATTCATAGGG GAAGTTTCAG ATGATCCTGT GGATTCGGGG 
TTGTTTTCCA ATAGAAACAA CAATGCTGGG TCTTTCGACT CGGAGGGTGT TGCCAGCAGG 
AGAGACTCCC TGGCGCCACT TCAAGCTGAG CACAGCCAGC CCCATGAAAA GGCAAGGGAG 
GAAGTACCTG CCCTGCACCC CGCTTCCCAT GACGTCGGAA AAGGAATCCG TGTGGCCTTA 
TCTAACATCT CTAAAGATGG GAATCTAATG GAAACGGCGC CCAGGGTGAC TTCTTTTGCT 
TCGAATCTTC ACACAGACAA CCTAAATGCA AAAGTGAAAG ACAAAGTGTA TGGCTGTGCT 
GACGGGGAGA GGACTCAAGC CACAGAGAGA GTGAATTCCC AACCGGTGAA TGAAAAGGAC 
AGCAACGATA AAAACGCTGC CTTGGCACCA ACATCATGGC ACCAACGAGG CCAAAACCCA 
GGGAAAAGCT ACAGACTTAA GCACGGCCTC ACAACGTATA AAATCATTCC TCCAAAGTCA 
GAGATGCGAT GTTACGACAG AGATGTGTCC CTCTCCACCG GAGCCATTAA GATTGACGAG 
CTGGGGAACT TGGTGAGTCC TCACGCCACC GGCATCAGGA TCATTTCCCT GTCTTCGTCT 
GTGCCTGAAG CAGAATCTCA GCCCATTGGG AAAGTCAGAG AGTTCTGGAG GTGCAACTCT 
GTGGAAAAAC ACCTGGGCCG ACCCTCAGAG AGCTCTGCCA GGGGACCCCC CTCCACCCCT 
GTGCCCACGC AGACGCAGAA TCCAGAGAGC AGACTCCAAG CAGACCCCAA GCCAATATCG 
CCCCAGCAGA AGTCTGCCCA CCATGAGGGC CGGAACCCCC TAGGGGAGGG GAGAAACCAG 
CCCCCCACCA TGGGCATGGG TCACGTGAGG GTGCCAGCAG CTCACACCAC AGAAGTCACA 
TTTCTCAAGC CTCAGAGAAG AACGTCCAGC CAGTATGTGG CCTCTGCCAT TGCCAAGCGC 
ATAGGAGCCC CAAAAGTCCA TGCTGATGTG GTGAGGCCAC ACGGTTATGC CGAGAAGGGT 
TATGCAGGCA AGGCGCCAGT GCTGGCTGCA CCACCTGTCA CTGTGAAAGA TGACAGGACA 
TCCAGCCCAC ACTCAGAGAC ACAAGGATGG AAGGATGGTG CCCAGTGGCC CTGTGTCACT 
CCTCCCAACA ACCACGGGGA AGATTTGGCA GTGGGAGCCC CTCCTAGGGG GGAGGTCATT 
GGCCCACACA GGAAGTTGTC TACTCAGGAC AGACCTGCTG CTATCCACAG AAGCTCCTGT 
TTCTCACTGG TTCAGTCTTC CCAGAGGGAT CGTGTTTCTG TGGGACAGAG CTGTGGTTTC 
AGTGGAAAGC AAAGCACAAG TAGCCAGGAG GCCAGCTCAG CATCTGAGCC CAGACGCGCA 
CCTGATGGTA CAGACCCACC CCCTCCACAC ACATCTGACA CTCAGGCCTG CAGCAGGGAG 
CTGGTCAATG GCTCTGTGCG CGCCCCAGGC CACGGCGAGC CTTCCCACCC TCCAGGAGGG 
TCTGGCACAG AAAGCCACAT TCTCCTAGAA AGAGAGGAAA AGCCCAGTGT GTTCTCTACA 
GATGGAAATG AAACAGACAG TATTTGGCCA CCCAGCATTT TTGGGCCGAA GAAAAAATTC 
AAACCTGTTG TCCAGAGACC AGTCCCAAAA GACACATCCC TGCACTCTGC CCTGATGGAA 
GCCATCCACT CTGCGGGAGG GAAGGACAGA CTACGCAAGA CGGCAGAACA CACCGGGGAG 
GGCAGGCCAG CGAAACTGTC CTACACGGAG GCAGAGGGCG AACGATCTGC ACTGCTGGCA 
GCTATCCGCG GGCACAGCGG CACCTGCAGC CTGAGGAAGG CTCTCTCTGC ACCAAGGACG 
GCCTCCAGGT TCAGCACGGG CACCCTCAGC AACACCGCAG ACGCAAGGCA AGCCTTGATG 
GACGCCATCC GCTCCGGCAC AGGGGCTGCG AGACTGAGAA AGGTGCCCTT GCTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_005271750.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271807.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

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CloneID	OHu41125
Clone ID Related Accession (Same CDS sequence)	XM_005271750.1
Accession Version	XM_005271750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGG CTGTTTAACG 
ACCCCCAACT CCCCATCCAT GCACTCACGT TCTCTTACGC TGGGTCCATC CCTCTCGCTG 
GGCAGCATCT CAGGGGTGTC CGTGAAGTCG GAGATGAAGA AGCGCCGAGC CCCTCCTCCT 
CCAGGTTCAG GGCCACCTGT GCAAGACAAG GCATCGGAAA AGGTATCTCT TGGGTCACAG 
ATTGATTTAC AGAAGAAGAA GCGGCGAGCG CCAGCTCCCC CTCCACCACA GCCACCACCA 
CCGAGTCCCC TGATCCCCAA CCGCACTGAG GATAAGGAGG AGAACAGGAA GAGCACGATG 
GGTGGTGGAC GGCAGGTGCC ACAAAAGCCT CCCAGAGGCA CTGCTCGGGG TCCACCCCAG 
TTAGTGCTTC CCCCACCCCC GCCCTACCCT CCTCCCGACA CAGACGTGGT GGAGCCTCTC 
AGCTTTCCCG GGGAAGGCGC TGGTTCCGAG GCCTCAGACC CGATACCCAA ACTAAGCCTG 
CCCCTGGGGT CTGGCAGCCA CTGCAGCCCG GATGGAGCCC CGCAGGTGCT GTCAGAGGCG 
GAGGAGACCG TGTCAGTTGG CAGCTGTTTT GCGTCGGAGG ACACGACCGA GGACTCAGGA 
GTGATGAGTT CCCCCTCAGA CATCGTCTCT CTGGACTCGC AGCAGGACAG CATGAAATAC 
AAAGACAAGT GGGCCACAGA CCAGGAAGAC TGCAGTGACC AGGACCTCGC TGGAACCCCA 
GACCTGGGTC CCCAGAAGAG CCCCTTGTGG GAGAAGAATG GCTCTGAGAA CTCACATCTG 
AGAACTGAAA AAGCTGTCAC AGCCTCAAAT GATGAAGAAG ACCTATTAAT TGCTGGAGAG 
TTCCGTAAAA CCCTTGCAGA ACTTGATGAA GACCTGGAAG AAATGGAAGA CAGCTATGAA 
ACAGACACCA GCTCCCTCAC CAGCTCCATC CATGGTGCAT CCAACCACTG CCCACAGGAT 
GCCATGATCC CTCACGGCGA CACAGATGCA ATCCCAGTAA CATTCATAGG GGAAGTTTCA 
GATGATCCTG TGGATTCGGG GTTGTTTTCC AATAGAAACA ACAATGCTGG GTCTTTCGAC 
TCGGAGGGTG TTGCCAGCAG GAGAGACTCC CTGGCGCCAC TTCAAGCTGA GCACAGCCAG 
CCCCATGAAA AGGCAAGGGA GGAAGTACCT GCCCTGCACC CCGCTTCCCA TGACGTCGGA 
AAAGGAATCC GTGTGGCCTT ATCTAACATC TCTAAAGATG GGAATCTAAT GGAAACGGCG 
CCCAGGGTGA CTTCTTTTGC TTCGAATCTT CACACAGACA ACCTAAATGC AAAAGTGAAA 
GACAAAGTGT ATGGCTGTGC TGACGGGGAG AGGACTCAAG CCACAGAGAG AGTGAATTCC 
CAACCGGTGA ATGAAAAGGA CAGCAACGAT AAAAACGCTG CCTTGGCACC AACATCATGG 
CACCAACGAG GCCAAAACCC AGGGAAAAGC TACAGACTTA AGCACGGCCT CACAACGTAT 
AAAATCATTC CTCCAAAGTC AGAGATGCGA TGTTACGACA GAGATGTGTC CCTCTCCACC 
GGAGCCATTA AGATTGACGA GCTGGGGAAC TTGGTGAGTC CTCACGCCAC CGGCATCAGG 
ATCATTTCCC TGTCTTCGTC TGTGCCTGAA GCAGAATCTC AGCCCATTGG GAAAGTCAGA 
GAGTTCTGGA GGTGCAACTC TGTGGAAAAA CACCTGGGCC GACCCTCAGA GAGCTCTGCC 
AGGGGACCCC CCTCCACCCC TGTGCCCACG CAGACGCAGA ATCCAGAGAG CAGACTCCAA 
GCAGACCCCA AGCCAATATC GCCCCAGCAG AAGTCTGCCC ACCATGAGGG CCGGAACCCC 
CTAGGGGAGG GGAGAAACCA GCCCCCCACC ATGGGCATGG GTCACGTGAG GGTGCCAGCA 
GCTCACACCA CAGAAGTCAC ATTTCTCAAG CCTCAGAGAA GAACGTCCAG CCAGTATGTG 
GCCTCTGCCA TTGCCAAGCG CATAGGAGCC CCAAAAGTCC ATGCTGATGT GGTGAGGCCA 
CACGGTTATG CCGAGAAGGG TTATGCAGGC AAGGCGCCAG TGCTGGCTGC ACCACCTGTC 
ACTGTGAAAG ATGACAGGAC ATCCAGCCCA CACTCAGAGA CACAAGGATG GAAGGATGGT 
GCCCAGTGGC CCTGTGTCAC TCCTCCCAAC AACCACGGGG AAGATTTGGC AGTGGGAGCC 
CCTCCTAGGG GGGAGGTCAT TGGCCCACAC AGGAAGTTGT CTACTCAGGA CAGACCTGCT 
GCTATCCACA GAAGCTCCTG TTTCTCACTG GTTCAGTCTT CCCAGAGGGA TCGTGTTTCT 
GTGGGACAGA GCTGTGGTTT CAGTGGAAAG CAAAGCACAA GTAGCCAGGA GGCCAGCTCA 
GCATCTGAGC CCAGACGCGC ACCTGATGGT ACAGACCCAC CCCCTCCACA CACATCTGAC 
ACTCAGGCCT GCAGCAGGGA GCTGGTCAAT GGCTCTGTGC GCGCCCCAGG CCACGGCGAG 
CCTTCCCACC CTCCAGGAGG GTCTGGCACA GAAAGCCACA TTCTCCTAGA AAGAGAGGAA 
AAGCCCAGTG TGTTCTCTAC AGATGGAAAT GAAACAGACA GTATTTGGCC ACCCAGCATT 
TTTGGGCCGA AGAAAAAATT CAAACCTGTT GTCCAGAGAC CAGTCCCAAA AGACACATCC 
CTGCACTCTG CCCTGATGGA AGCCATCCAC TCTGCGGGAG GGAAGGACAG ACTACGCAAG 
ACGGCAGAAC ACACCGGGGA GGGCAGGCCA GCGAAACTGT CCTACACGGA GGCAGAGGGC 
GAACGATCTG CACTGCTGGC AGCTATCCGC GGGCACAGCG GCACCTGCAG CCTGAGGAAG 
GTGGCATCCT CTGCTTCTGA GGAGCTCCAG AGCTTCCGAG ATGCCGCACT CTCTGCTCAG 
GGCTCGGAAA GTCCTCTGCT AGAAGACCTT GGTCTTCTGT CCCCACCAGC CATTCCCCCA 
CCACCCCCTC CACCCTCCCA GGCTCTCTCT GCACCAAGGA CGGCCTCCAG GTTCAGCACG 
GGCACCCTCA GCAACACCGC AGACGCAAGG CAAGCCTTGA TGGACGCCAT CCGCTCCGGC 
ACAGGGGCTG CGAGACTGAG AAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271807.1
CDS	177..4178
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_005271750.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271750.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGG CTGTTTAACG 
ACCCCCAACT CCCCATCCAT GCACTCACGT TCTCTTACGC TGGGTCCATC CCTCTCGCTG 
GGCAGCATCT CAGGGGTGTC CGTGAAGTCG GAGATGAAGA AGCGCCGAGC CCCTCCTCCT 
CCAGGTTCAG GGCCACCTGT GCAAGACAAG GCATCGGAAA AGGTATCTCT TGGGTCACAG 
ATTGATTTAC AGAAGAAGAA GCGGCGAGCG CCAGCTCCCC CTCCACCACA GCCACCACCA 
CCGAGTCCCC TGATCCCCAA CCGCACTGAG GATAAGGAGG AGAACAGGAA GAGCACGATG 
GGTGGTGGAC GGCAGGTGCC ACAAAAGCCT CCCAGAGGCA CTGCTCGGGG TCCACCCCAG 
TTAGTGCTTC CCCCACCCCC GCCCTACCCT CCTCCCGACA CAGACGTGGT GGAGCCTCTC 
AGCTTTCCCG GGGAAGGCGC TGGTTCCGAG GCCTCAGACC CGATACCCAA ACTAAGCCTG 
CCCCTGGGGT CTGGCAGCCA CTGCAGCCCG GATGGAGCCC CGCAGGTGCT GTCAGAGGCG 
GAGGAGACCG TGTCAGTTGG CAGCTGTTTT GCGTCGGAGG ACACGACCGA GGACTCAGGA 
GTGATGAGTT CCCCCTCAGA CATCGTCTCT CTGGACTCGC AGCAGGACAG CATGAAATAC 
AAAGACAAGT GGGCCACAGA CCAGGAAGAC TGCAGTGACC AGGACCTCGC TGGAACCCCA 
GACCTGGGTC CCCAGAAGAG CCCCTTGTGG GAGAAGAATG GCTCTGAGAA CTCACATCTG 
AGAACTGAAA AAGCTGTCAC AGCCTCAAAT GATGAAGAAG ACCTATTAAT TGCTGGAGAG 
TTCCGTAAAA CCCTTGCAGA ACTTGATGAA GACCTGGAAG AAATGGAAGA CAGCTATGAA 
ACAGACACCA GCTCCCTCAC CAGCTCCATC CATGGTGCAT CCAACCACTG CCCACAGGAT 
GCCATGATCC CTCACGGCGA CACAGATGCA ATCCCAGTAA CATTCATAGG GGAAGTTTCA 
GATGATCCTG TGGATTCGGG GTTGTTTTCC AATAGAAACA ACAATGCTGG GTCTTTCGAC 
TCGGAGGGTG TTGCCAGCAG GAGAGACTCC CTGGCGCCAC TTCAAGCTGA GCACAGCCAG 
CCCCATGAAA AGGCAAGGGA GGAAGTACCT GCCCTGCACC CCGCTTCCCA TGACGTCGGA 
AAAGGAATCC GTGTGGCCTT ATCTAACATC TCTAAAGATG GGAATCTAAT GGAAACGGCG 
CCCAGGGTGA CTTCTTTTGC TTCGAATCTT CACACAGACA ACCTAAATGC AAAAGTGAAA 
GACAAAGTGT ATGGCTGTGC TGACGGGGAG AGGACTCAAG CCACAGAGAG AGTGAATTCC 
CAACCGGTGA ATGAAAAGGA CAGCAACGAT AAAAACGCTG CCTTGGCACC AACATCATGG 
CACCAACGAG GCCAAAACCC AGGGAAAAGC TACAGACTTA AGCACGGCCT CACAACGTAT 
AAAATCATTC CTCCAAAGTC AGAGATGCGA TGTTACGACA GAGATGTGTC CCTCTCCACC 
GGAGCCATTA AGATTGACGA GCTGGGGAAC TTGGTGAGTC CTCACGCCAC CGGCATCAGG 
ATCATTTCCC TGTCTTCGTC TGTGCCTGAA GCAGAATCTC AGCCCATTGG GAAAGTCAGA 
GAGTTCTGGA GGTGCAACTC TGTGGAAAAA CACCTGGGCC GACCCTCAGA GAGCTCTGCC 
AGGGGACCCC CCTCCACCCC TGTGCCCACG CAGACGCAGA ATCCAGAGAG CAGACTCCAA 
GCAGACCCCA AGCCAATATC GCCCCAGCAG AAGTCTGCCC ACCATGAGGG CCGGAACCCC 
CTAGGGGAGG GGAGAAACCA GCCCCCCACC ATGGGCATGG GTCACGTGAG GGTGCCAGCA 
GCTCACACCA CAGAAGTCAC ATTTCTCAAG CCTCAGAGAA GAACGTCCAG CCAGTATGTG 
GCCTCTGCCA TTGCCAAGCG CATAGGAGCC CCAAAAGTCC ATGCTGATGT GGTGAGGCCA 
CACGGTTATG CCGAGAAGGG TTATGCAGGC AAGGCGCCAG TGCTGGCTGC ACCACCTGTC 
ACTGTGAAAG ATGACAGGAC ATCCAGCCCA CACTCAGAGA CACAAGGATG GAAGGATGGT 
GCCCAGTGGC CCTGTGTCAC TCCTCCCAAC AACCACGGGG AAGATTTGGC AGTGGGAGCC 
CCTCCTAGGG GGGAGGTCAT TGGCCCACAC AGGAAGTTGT CTACTCAGGA CAGACCTGCT 
GCTATCCACA GAAGCTCCTG TTTCTCACTG GTTCAGTCTT CCCAGAGGGA TCGTGTTTCT 
GTGGGACAGA GCTGTGGTTT CAGTGGAAAG CAAAGCACAA GTAGCCAGGA GGCCAGCTCA 
GCATCTGAGC CCAGACGCGC ACCTGATGGT ACAGACCCAC CCCCTCCACA CACATCTGAC 
ACTCAGGCCT GCAGCAGGGA GCTGGTCAAT GGCTCTGTGC GCGCCCCAGG CCACGGCGAG 
CCTTCCCACC CTCCAGGAGG GTCTGGCACA GAAAGCCACA TTCTCCTAGA AAGAGAGGAA 
AAGCCCAGTG TGTTCTCTAC AGATGGAAAT GAAACAGACA GTATTTGGCC ACCCAGCATT 
TTTGGGCCGA AGAAAAAATT CAAACCTGTT GTCCAGAGAC CAGTCCCAAA AGACACATCC 
CTGCACTCTG CCCTGATGGA AGCCATCCAC TCTGCGGGAG GGAAGGACAG ACTACGCAAG 
ACGGCAGAAC ACACCGGGGA GGGCAGGCCA GCGAAACTGT CCTACACGGA GGCAGAGGGC 
GAACGATCTG CACTGCTGGC AGCTATCCGC GGGCACAGCG GCACCTGCAG CCTGAGGAAG 
GTGGCATCCT CTGCTTCTGA GGAGCTCCAG AGCTTCCGAG ATGCCGCACT CTCTGCTCAG 
GGCTCGGAAA GTCCTCTGCT AGAAGACCTT GGTCTTCTGT CCCCACCAGC CATTCCCCCA 
CCACCCCCTC CACCCTCCCA GGCTCTCTCT GCACCAAGGA CGGCCTCCAG GTTCAGCACG 
GGCACCCTCA GCAACACCGC AGACGCAAGG CAAGCCTTGA TGGACGCCAT CCGCTCCGGC 
ACAGGGGCTG CGAGACTGAG AAAGGTGCCC TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515234.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513536.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu65777
Clone ID Related Accession (Same CDS sequence)	XM_011515234.1
Accession Version	XM_011515234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGGC ATCCTCTGCT TCTGAGGAGC TCCAGAGCTT CCGAGATGCC 
GCACTCTCTG CTCAGGGCTC GGAAAGTCCT CTGCTAGAAG ACCTTGGTCT TCTGTCCCCA 
CCAGCCATTC CCCCACCACC CCCTCCACCC TCCCAGGCTC TCTCTGCACC AAGGACGGCC 
TCCAGGTTCA GCACGGGCAC CCTCAGCAAC ACCGCAGACG CAAGGCAAGC CTTGATGGAC 
GCCATCCGCT CCGGCACAGG GGCTGCGAGA CTGAGAAAGG TGCCCTTGCT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513536.1
CDS	177..4253
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRVRLSKTQSEPSLSCQETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515234.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGTTCGT CTGAGCAAAA CTCAGTCCGA GCCTTCCCTG 
AGCTGTCAAG AAACCTTTAG GAAATCATCA CTTGGCAATG ATGAGACAGA TAAAGAGAAG 
AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAGTAAGGC TGAGCAGCTC 
GTGCTGTCGG GTGCAGACAG CGATGAGGAC ACCTCCAGGG CTGCCCCAGG AAGGGGTTTG 
AACGGCTGTT TAACGACCCC CAACTCCCCA TCCATGCACT CACGTTCTCT TACGCTGGGT 
CCATCCCTCT CGCTGGGCAG CATCTCAGGG GTGTCCGTGA AGTCGGAGAT GAAGAAGCGC 
CGAGCCCCTC CTCCTCCAGG TTCAGGGCCA CCTGTGCAAG ACAAGGCATC GGAAAAGGTA 
TCTCTTGGGT CACAGATTGA TTTACAGAAG AAGAAGCGGC GAGCGCCAGC TCCCCCTCCA 
CCACAGCCAC CACCACCGAG TCCCCTGATC CCCAACCGCA CTGAGGATAA GGAGGAGAAC 
AGGAAGAGCA CGATGGGTGG TGGACGGCAG GTGCCACAAA AGCCTCCCAG AGGCACTGCT 
CGGGGTCCAC CCCAGTTAGT GCTTCCCCCA CCCCCGCCCT ACCCTCCTCC CGACACAGAC 
GTGGTGGAGC CTCTCAGCTT TCCCGGGGAA GGCGCTGGTT CCGAGGCCTC AGACCCGATA 
CCCAAACTAA GCCTGCCCCT GGGGTCTGGC AGCCACTGCA GCCCGGATGG AGCCCCGCAG 
GTGCTGTCAG AGGCGGAGGA GACCGTGTCA GTTGGCAGCT GTTTTGCGTC GGAGGACACG 
ACCGAGGACT CAGGAGTGAT GAGTTCCCCC TCAGACATCG TCTCTCTGGA CTCGCAGCAG 
GACAGCATGA AATACAAAGA CAAGTGGGCC ACAGACCAGG AAGACTGCAG TGACCAGGAC 
CTCGCTGGAA CCCCAGACCT GGGTCCCCAG AAGAGCCCCT TGTGGGAGAA GAATGGCTCT 
GAGAACTCAC ATCTGAGAAC TGAAAAAGCT GTCACAGCCT CAAATGATGA AGAAGACCTA 
TTAATTGCTG GAGAGTTCCG TAAAACCCTT GCAGAACTTG ATGAAGACCT GGAAGAAATG 
GAAGACAGCT ATGAAACAGA CACCAGCTCC CTCACCAGCT CCATCCATGG TGCATCCAAC 
CACTGCCCAC AGGATGCCAT GATCCCTCAC GGCGACACAG ATGCAATCCC AGTAACATTC 
ATAGGGGAAG TTTCAGATGA TCCTGTGGAT TCGGGGTTGT TTTCCAATAG AAACAACAAT 
GCTGGGTCTT TCGACTCGGA GGGTGTTGCC AGCAGGAGAG ACTCCCTGGC GCCACTTCAA 
GCTGAGCACA GCCAGCCCCA TGAAAAGGCA AGGGAGGAAG TACCTGCCCT GCACCCCGCT 
TCCCATGACG TCGGAAAAGG AATCCGTGTG GCCTTATCTA ACATCTCTAA AGATGGGAAT 
CTAATGGAAA CGGCGCCCAG GGTGACTTCT TTTGCTTCGA ATCTTCACAC AGACAACCTA 
AATGCAAAAG TGAAAGACAA AGTGTATGGC TGTGCTGACG GGGAGAGGAC TCAAGCCACA 
GAGAGAGTGA ATTCCCAACC GGTGAATGAA AAGGACAGCA ACGATAAAAA CGCTGCCTTG 
GCACCAACAT CATGGCACCA ACGAGGCCAA AACCCAGGGA AAAGCTACAG ACTTAAGCAC 
GGCCTCACAA CGTATAAAAT CATTCCTCCA AAGTCAGAGA TGCGATGTTA CGACAGAGAT 
GTGTCCCTCT CCACCGGAGC CATTAAGATT GACGAGCTGG GGAACTTGGT GAGTCCTCAC 
GCCACCGGCA TCAGGATCAT TTCCCTGTCT TCGTCTGTGC CTGAAGCAGA ATCTCAGCCC 
ATTGGGAAAG TCAGAGAGTT CTGGAGGTGC AACTCTGTGG AAAAACACCT GGGCCGACCC 
TCAGAGAGCT CTGCCAGGGG ACCCCCCTCC ACCCCTGTGC CCACGCAGAC GCAGAATCCA 
GAGAGCAGAC TCCAAGCAGA CCCCAAGCCA ATATCGCCCC AGCAGAAGTC TGCCCACCAT 
GAGGGCCGGA ACCCCCTAGG GGAGGGGAGA AACCAGCCCC CCACCATGGG CATGGGTCAC 
GTGAGGGTGC CAGCAGCTCA CACCACAGAA GTCACATTTC TCAAGCCTCA GAGAAGAACG 
TCCAGCCAGT ATGTGGCCTC TGCCATTGCC AAGCGCATAG GAGCCCCAAA AGTCCATGCT 
GATGTGGTGA GGCCACACGG TTATGCCGAG AAGGGTTATG CAGGCAAGGC GCCAGTGCTG 
GCTGCACCAC CTGTCACTGT GAAAGATGAC AGGACATCCA GCCCACACTC AGAGACACAA 
GGATGGAAGG ATGGTGCCCA GTGGCCCTGT GTCACTCCTC CCAACAACCA CGGGGAAGAT 
TTGGCAGTGG GAGCCCCTCC TAGGGGGGAG GTCATTGGCC CACACAGGAA GTTGTCTACT 
CAGGACAGAC CTGCTGCTAT CCACAGAAGC TCCTGTTTCT CACTGGTTCA GTCTTCCCAG 
AGGGATCGTG TTTCTGTGGG ACAGAGCTGT GGTTTCAGTG GAAAGCAAAG CACAAGTAGC 
CAGGAGGCCA GCTCAGCATC TGAGCCCAGA CGCGCACCTG ATGGTACAGA CCCACCCCCT 
CCACACACAT CTGACACTCA GGCCTGCAGC AGGGAGCTGG TCAATGGCTC TGTGCGCGCC 
CCAGGCCACG GCGAGCCTTC CCACCCTCCA GGAGGGTCTG GCACAGAAAG CCACATTCTC 
CTAGAAAGAG AGGAAAAGCC CAGTGTGTTC TCTACAGATG GAAATGAAAC AGACAGTATT 
TGGCCACCCA GCATTTTTGG GCCGAAGAAA AAATTCAAAC CTGTTGTCCA GAGACCAGTC 
CCAAAAGACA CATCCCTGCA CTCTGCCCTG ATGGAAGCCA TCCACTCTGC GGGAGGGAAG 
GACAGACTAC GCAAGACGGC AGAACACACC GGGGAGGGCA GGCCAGCGAA ACTGTCCTAC 
ACGGAGGCAG AGGGCGAACG ATCTGCACTG CTGGCAGCTA TCCGCGGGCA CAGCGGCACC 
TGCAGCCTGA GGAAGGTGGC ATCCTCTGCT TCTGAGGAGC TCCAGAGCTT CCGAGATGCC 
GCACTCTCTG CTCAGGGCTC GGAAAGTCCT CTGCTAGAAG ACCTTGGTCT TCTGTCCCCA 
CCAGCCATTC CCCCACCACC CCCTCCACCC TCCCAGGCTC TCTCTGCACC AAGGACGGCC 
TCCAGGTTCA GCACGGGCAC CCTCAGCAAC ACCGCAGACG CAAGGCAAGC CTTGATGGAC 
GCCATCCGCT CCGGCACAGG GGCTGCGAGA CTGAGAAAGG TGCCCTTGCT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COBL ( XM_011515235.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513537.1 Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu65778
Clone ID Related Accession (Same CDS sequence)	XM_011515235.1
Accession Version	XM_011515235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein cordon-bleu isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGCTGAGC AGCTCGTGCT GTCGGGTGCA GACAGCGATG AGGACACCTC CAGGGCTGCC 
CCAGGAAGGG GTTTGAACGG CTGTTTAACG ACCCCCAACT CCCCATCCAT GCACTCACGT 
TCTCTTACGC TGGGTCCATC CCTCTCGCTG GGCAGCATCT CAGGGGTGTC CGTGAAGTCG 
GAGATGAAGA AGCGCCGAGC CCCTCCTCCT CCAGGTTCAG GGCCACCTGT GCAAGACAAG 
GCATCGGAAA AGGTATCTCT TGGGTCACAG ATTGATTTAC AGAAGAAGAA GCGGCGAGCG 
CCAGCTCCCC CTCCACCACA GCCACCACCA CCGAGTCCCC TGATCCCCAA CCGCACTGAG 
GATAAGGAGG AGAACAGGAA GAGCACGATG GGTGGTGGAC GGCAGGTGCC ACAAAAGCCT 
CCCAGAGGCA CTGCTCGGGG TCCACCCCAG TTAGTGCTTC CCCCACCCCC GCCCTACCCT 
CCTCCCGACA CAGACGTGGT GGAGCCTCTC AGCTTTCCCG GGGAAGGCGC TGGTTCCGAG 
GCCTCAGACC CGATACCCAA ACTAAGCCTG CCCCTGGGGT CTGGCAGCCA CTGCAGCCCG 
GATGGAGCCC CGCAGGTGCT GTCAGAGGCG GAGGAGACCG TGTCAGTTGG CAGCTGTTTT 
GCGTCGGAGG ACACGACCGA GGACTCAGGA GTGATGAGTT CCCCCTCAGA CATCGTCTCT 
CTGGACTCGC AGCAGGACAG CATGAAATAC AAAGACAAGT GGGCCACAGA CCAGGAAGAC 
TGCAGTGACC AGGACCTCGC TGGAACCCCA GACCTGGGTC CCCAGAAGAG CCCCTTGTGG 
GAGAAGAATG GCTCTGAGAA CTCACATCTG AGAACTGAAA AAGCTGTCAC AGCCTCAAAT 
GATGAAGAAG ACCTATTAAT TGCTGGAGAG TTCCGTAAAA CCCTTGCAGA ACTTGATGAA 
GACCTGGAAG AAATGGAAGA CAGCTATGAA ACAGACACCA GCTCCCTCAC CAGCTCCATC 
CATGGTGCAT CCAACCACTG CCCACAGGAT GCCATGATCC CTCACGGCGA CACAGATGCA 
ATCCCAGTAA CATTCATAGG GGAAGTTTCA GATGATCCTG TGGATTCGGG GTTGTTTTCC 
AATAGAAACA ACAATGCTGG GTCTTTCGAC TCGGAGGGTG TTGCCAGCAG GAGAGACTCC 
CTGGCGCCAC TTCAAGCTGA GCACAGCCAG CCCCATGAAA AGGCAAGGGA GGAAGTACCT 
GCCCTGCACC CCGCTTCCCA TGACGTCGGA AAAGGAATCC GTGTGGCCTT ATCTAACATC 
TCTAAAGATG GGAATCTAAT GGAAACGGCG CCCAGGGTGA CTTCTTTTGC TTCGAATCTT 
CACACAGACA ACCTAAATGC AAAAGTGAAA GACAAAGTGT ATGGCTGTGC TGACGGGGAG 
AGGACTCAAG CCACAGAGAG AGTGAATTCC CAACCGGTGA ATGAAAAGGA CAGCAACGAT 
AAAAACGCTG CCTTGGCACC AACATCATGG CACCAACGAG GCCAAAACCC AGGGAAAAGC 
TACAGACTTA AGCACGGCCT CACAACGTAT AAAATCATTC CTCCAAAGTC AGAGATGCGA 
TGTTACGACA GAGATGTGTC CCTCTCCACC GGAGCCATTA AGATTGACGA GCTGGGGAAC 
TTGGTGAGTC CTCACGCCAC CGGCATCAGG ATCATTTCCC TGTCTTCGTC TGTGCCTGAA 
GCAGAATCTC AGCCCATTGG GAAAGTCAGA GAGTTCTGGA GGTGCAACTC TGTGGAAAAA 
CACCTGGGCC GACCCTCAGA GAGCTCTGCC AGGGGACCCC CCTCCACCCC TGTGCCCACG 
CAGACGCAGA ATCCAGAGAG CAGACTCCAA GCAGACCCCA AGCCAATATC GCCCCAGCAG 
AAGTCTGCCC ACCATGAGGG CCGGAACCCC CTAGGGGAGG GGAGAAACCA GCCCCCCACC 
ATGGGCATGG GTCACGTGAG GGTGCCAGCA GCTCACACCA CAGAAGTCAC ATTTCTCAAG 
CCTCAGAGAA GAACGTCCAG CCAGTATGTG GCCTCTGCCA TTGCCAAGCG CATAGGAGCC 
CCAAAAGTCC ATGCTGATGT GGTGAGGCCA CACGGTTATG CCGAGAAGGG TTATGCAGGC 
AAGGCGCCAG TGCTGGCTGC ACCACCTGTC ACTGTGAAAG ATGACAGGAC ATCCAGCCCA 
CACTCAGAGA CACAAGGATG GAAGGATGGT GCCCAGTGGC CCTGTGTCAC TCCTCCCAAC 
AACCACGGGG AAGATTTGGC AGTGGGAGCC CCTCCTAGGG GGGAGGTCAT TGGCCCACAC 
AGGAAGTTGT CTACTCAGGA CAGACCTGCT GCTATCCACA GAAGCTCCTG TTTCTCACTG 
GTTCAGTCTT CCCAGAGGGA TCGTGTTTCT GTGGGACAGA GCTGTGGTTT CAGTGGAAAG 
CAAAGCACAA GTAGCCAGGA GGCCAGCTCA GCATCTGAGC CCAGACGCGC ACCTGATGGT 
ACAGACCCAC CCCCTCCACA CACATCTGAC ACTCAGGCCT GCAGCAGGGA GCTGGTCAAT 
GGCTCTGTGC GCGCCCCAGG CCACGGCGAG CCTTCCCACC CTCCAGGAGG GTCTGGCACA 
GAAAGCCACA TTCTCCTAGA AAGAGAGGAA AAGCCCAGTG TGTTCTCTAC AGATGGAAAT 
GAAACAGACA GTATTTGGCC ACCCAGCATT TTTGGGCCGA AGAAAAAATT CAAACCTGTT 
GTCCAGAGAC CAGTCCCAAA AGACACATCC CTGCACTCTG CCCTGATGGA AGCCATCCAC 
TCTGCGGGAG GGAAGGACAG ACTACGCAAG ACGGCAGAAC ACACCGGGGA GGGCAGGCCA 
GCGAAACTGT CCTACACGGA GGCAGAGGGC GAACGATCTG CACTGCTGGC AGCTATCCGC 
GGGCACAGCG GCACCTGCAG CCTGAGGAAG GTGGCATCCT CTGCTTCTGA GGAGCTCCAG 
AGCTTCCGAG ATGCCGCACT CTCTGCTCAG GGCTCGGAAA GTCCTCTGCT AGAAGACCTT 
GGTCTTCTGT CCCCACCAGC CATTCCCCCA CCACCCCCTC CACCCTCCCA GGCTCTCTCT 
GCACCAAGGA CGGCCTCCAG GTTCAGCACG GGCACCCTCA GCAACACCGC AGACGCAAGG 
CAAGCCTTGA TGGACGCCAT CCGCTCCGGC ACAGGGGCTG CGAGACTGAG AAAGGTGCCC 
TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513537.1
CDS	177..4208
Translation	MDAPRASAAKPPTGRKMKARAPPPPGKAATLHVHSDQKPPHDGALGSQQNLVRMKEALRASTMDVTVVLPSGLEKRSVLNGSHAMMDLLVELCLQNHLNPSHHALEIRSSETQQPLSFKPNTLIGTLNVHTVFLKEKVPEEKVKPGPPKVPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVSPEHVVLLRDNIAGEELELSKSLNELGIKELYAWDNRRETFRKSSLGNDETDKEKKKFLGFFKVNKRSNSKAEQLVLSGADSDEDTSRAAPGRGLNGCLTTPNSPSMHSRSLTLGPSLSLGSISGVSVKSEMKKRRAPPPPGSGPPVQDKASEKVSLGSQIDLQKKKRRAPAPPPPQPPPPSPLIPNRTEDKEENRKSTMGGGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVVEPLSFPGEGAGSEASDPIPKLSLPLGSGSHCSPDGAPQVLSEAEETVSVGSCFASEDTTEDSGVMSSPSDIVSLDSQQDSMKYKDKWATDQEDCSDQDLAGTPDLGPQKSPLWEKNGSENSHLRTEKAVTASNDEEDLLIAGEFRKTLAELDEDLEEMEDSYETDTSSLTSSIHGASNHCPQDAMIPHGDTDAIPVTFIGEVSDDPVDSGLFSNRNNNAGSFDSEGVASRRDSLAPLQAEHSQPHEKAREEVPALHPASHDVGKGIRVALSNISKDGNLMETAPRVTSFASNLHTDNLNAKVKDKVYGCADGERTQATERVNSQPVNEKDSNDKNAALAPTSWHQRGQNPGKSYRLKHGLTTYKIIPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHATGIRIISLSSSVPEAESQPIGKVREFWRCNSVEKHLGRPSESSARGPPSTPVPTQTQNPESRLQADPKPISPQQKSAHHEGRNPLGEGRNQPPTMGMGHVRVPAAHTTEVTFLKPQRRTSSQYVASAIAKRIGAPKVHADVVRPHGYAEKGYAGKAPVLAAPPVTVKDDRTSSPHSETQGWKDGAQWPCVTPPNNHGEDLAVGAPPRGEVIGPHRKLSTQDRPAAIHRSSCFSLVQSSQRDRVSVGQSCGFSGKQSTSSQEASSASEPRRAPDGTDPPPPHTSDTQACSRELVNGSVRAPGHGEPSHPPGGSGTESHILLEREEKPSVFSTDGNETDSIWPPSIFGPKKKFKPVVQRPVPKDTSLHSALMEAIHSAGGKDRLRKTAEHTGEGRPAKLSYTEAEGERSALLAAIRGHSGTCSLRKVASSASEELQSFRDAALSAQGSESPLLEDLGLLSPPAIPPPPPPPSQALSAPRTASRFSTGTLSNTADARQALMDAIRSGTGAARLRKVPLLV

Target ORF information:

RefSeq Version	XM_011515235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515235.1

ORF Insert Sequence:

ATGGACGCGC CGCGCGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGT 
GCTCCCCCAC CTCCTGGAAA GGCTGCCACT CTGCATGTGC ACAGTGACCA GAAGCCCCCC 
CACGATGGGG CCCTCGGGTC GCAGCAGAAC TTGGTTCGCA TGAAGGAGGC GCTGAGGGCC 
AGCACCATGG ACGTCACCGT GGTCCTGCCT AGTGGGCTGG AGAAGAGGAG CGTGCTCAAT 
GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCA 
TCCCACCATG CCCTTGAAAT TCGGTCTTCA GAAACCCAAC AACCTTTGAG TTTTAAGCCA 
AATACTTTGA TTGGGACCCT GAATGTGCAT ACTGTGTTTC TGAAAGAAAA AGTTCCTGAA 
GAGAAGGTTA AGCCTGGTCC CCCTAAGGTG CCTGAGAAAT CTGTGCGTTT GGTCGTGAAT 
TACCTGCGGA CACAAAAAGC TGTTGTGCGT GTGAGCCCTG AGGTTCCTCT CCAGAATATT 
CTCCCAGTCA TTTGTGCAAA GTGTGAGGTC AGCCCAGAGC ACGTGGTTCT CCTCAGGGAC 
AACATTGCCG GAGAGGAGCT GGAGCTGTCC AAGTCCCTGA ACGAGCTCGG GATAAAGGAG 
CTCTACGCGT GGGACAACAG AAGAGAAACC TTTAGGAAAT CATCACTTGG CAATGATGAG 
ACAGATAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAGT 
AAGGCTGAGC AGCTCGTGCT GTCGGGTGCA GACAGCGATG AGGACACCTC CAGGGCTGCC 
CCAGGAAGGG GTTTGAACGG CTGTTTAACG ACCCCCAACT CCCCATCCAT GCACTCACGT 
TCTCTTACGC TGGGTCCATC CCTCTCGCTG GGCAGCATCT CAGGGGTGTC CGTGAAGTCG 
GAGATGAAGA AGCGCCGAGC CCCTCCTCCT CCAGGTTCAG GGCCACCTGT GCAAGACAAG 
GCATCGGAAA AGGTATCTCT TGGGTCACAG ATTGATTTAC AGAAGAAGAA GCGGCGAGCG 
CCAGCTCCCC CTCCACCACA GCCACCACCA CCGAGTCCCC TGATCCCCAA CCGCACTGAG 
GATAAGGAGG AGAACAGGAA GAGCACGATG GGTGGTGGAC GGCAGGTGCC ACAAAAGCCT 
CCCAGAGGCA CTGCTCGGGG TCCACCCCAG TTAGTGCTTC CCCCACCCCC GCCCTACCCT 
CCTCCCGACA CAGACGTGGT GGAGCCTCTC AGCTTTCCCG GGGAAGGCGC TGGTTCCGAG 
GCCTCAGACC CGATACCCAA ACTAAGCCTG CCCCTGGGGT CTGGCAGCCA CTGCAGCCCG 
GATGGAGCCC CGCAGGTGCT GTCAGAGGCG GAGGAGACCG TGTCAGTTGG CAGCTGTTTT 
GCGTCGGAGG ACACGACCGA GGACTCAGGA GTGATGAGTT CCCCCTCAGA CATCGTCTCT 
CTGGACTCGC AGCAGGACAG CATGAAATAC AAAGACAAGT GGGCCACAGA CCAGGAAGAC 
TGCAGTGACC AGGACCTCGC TGGAACCCCA GACCTGGGTC CCCAGAAGAG CCCCTTGTGG 
GAGAAGAATG GCTCTGAGAA CTCACATCTG AGAACTGAAA AAGCTGTCAC AGCCTCAAAT 
GATGAAGAAG ACCTATTAAT TGCTGGAGAG TTCCGTAAAA CCCTTGCAGA ACTTGATGAA 
GACCTGGAAG AAATGGAAGA CAGCTATGAA ACAGACACCA GCTCCCTCAC CAGCTCCATC 
CATGGTGCAT CCAACCACTG CCCACAGGAT GCCATGATCC CTCACGGCGA CACAGATGCA 
ATCCCAGTAA CATTCATAGG GGAAGTTTCA GATGATCCTG TGGATTCGGG GTTGTTTTCC 
AATAGAAACA ACAATGCTGG GTCTTTCGAC TCGGAGGGTG TTGCCAGCAG GAGAGACTCC 
CTGGCGCCAC TTCAAGCTGA GCACAGCCAG CCCCATGAAA AGGCAAGGGA GGAAGTACCT 
GCCCTGCACC CCGCTTCCCA TGACGTCGGA AAAGGAATCC GTGTGGCCTT ATCTAACATC 
TCTAAAGATG GGAATCTAAT GGAAACGGCG CCCAGGGTGA CTTCTTTTGC TTCGAATCTT 
CACACAGACA ACCTAAATGC AAAAGTGAAA GACAAAGTGT ATGGCTGTGC TGACGGGGAG 
AGGACTCAAG CCACAGAGAG AGTGAATTCC CAACCGGTGA ATGAAAAGGA CAGCAACGAT 
AAAAACGCTG CCTTGGCACC AACATCATGG CACCAACGAG GCCAAAACCC AGGGAAAAGC 
TACAGACTTA AGCACGGCCT CACAACGTAT AAAATCATTC CTCCAAAGTC AGAGATGCGA 
TGTTACGACA GAGATGTGTC CCTCTCCACC GGAGCCATTA AGATTGACGA GCTGGGGAAC 
TTGGTGAGTC CTCACGCCAC CGGCATCAGG ATCATTTCCC TGTCTTCGTC TGTGCCTGAA 
GCAGAATCTC AGCCCATTGG GAAAGTCAGA GAGTTCTGGA GGTGCAACTC TGTGGAAAAA 
CACCTGGGCC GACCCTCAGA GAGCTCTGCC AGGGGACCCC CCTCCACCCC TGTGCCCACG 
CAGACGCAGA ATCCAGAGAG CAGACTCCAA GCAGACCCCA AGCCAATATC GCCCCAGCAG 
AAGTCTGCCC ACCATGAGGG CCGGAACCCC CTAGGGGAGG GGAGAAACCA GCCCCCCACC 
ATGGGCATGG GTCACGTGAG GGTGCCAGCA GCTCACACCA CAGAAGTCAC ATTTCTCAAG 
CCTCAGAGAA GAACGTCCAG CCAGTATGTG GCCTCTGCCA TTGCCAAGCG CATAGGAGCC 
CCAAAAGTCC ATGCTGATGT GGTGAGGCCA CACGGTTATG CCGAGAAGGG TTATGCAGGC 
AAGGCGCCAG TGCTGGCTGC ACCACCTGTC ACTGTGAAAG ATGACAGGAC ATCCAGCCCA 
CACTCAGAGA CACAAGGATG GAAGGATGGT GCCCAGTGGC CCTGTGTCAC TCCTCCCAAC 
AACCACGGGG AAGATTTGGC AGTGGGAGCC CCTCCTAGGG GGGAGGTCAT TGGCCCACAC 
AGGAAGTTGT CTACTCAGGA CAGACCTGCT GCTATCCACA GAAGCTCCTG TTTCTCACTG 
GTTCAGTCTT CCCAGAGGGA TCGTGTTTCT GTGGGACAGA GCTGTGGTTT CAGTGGAAAG 
CAAAGCACAA GTAGCCAGGA GGCCAGCTCA GCATCTGAGC CCAGACGCGC ACCTGATGGT 
ACAGACCCAC CCCCTCCACA CACATCTGAC ACTCAGGCCT GCAGCAGGGA GCTGGTCAAT 
GGCTCTGTGC GCGCCCCAGG CCACGGCGAG CCTTCCCACC CTCCAGGAGG GTCTGGCACA 
GAAAGCCACA TTCTCCTAGA AAGAGAGGAA AAGCCCAGTG TGTTCTCTAC AGATGGAAAT 
GAAACAGACA GTATTTGGCC ACCCAGCATT TTTGGGCCGA AGAAAAAATT CAAACCTGTT 
GTCCAGAGAC CAGTCCCAAA AGACACATCC CTGCACTCTG CCCTGATGGA AGCCATCCAC 
TCTGCGGGAG GGAAGGACAG ACTACGCAAG ACGGCAGAAC ACACCGGGGA GGGCAGGCCA 
GCGAAACTGT CCTACACGGA GGCAGAGGGC GAACGATCTG CACTGCTGGC AGCTATCCGC 
GGGCACAGCG GCACCTGCAG CCTGAGGAAG GTGGCATCCT CTGCTTCTGA GGAGCTCCAG 
AGCTTCCGAG ATGCCGCACT CTCTGCTCAG GGCTCGGAAA GTCCTCTGCT AGAAGACCTT 
GGTCTTCTGT CCCCACCAGC CATTCCCCCA CCACCCCCTC CACCCTCCCA GGCTCTCTCT 
GCACCAAGGA CGGCCTCCAG GTTCAGCACG GGCACCCTCA GCAACACCGC AGACGCAAGG 
CAAGCCTTGA TGGACGCCAT CCGCTCCGGC ACAGGGGCTG CGAGACTGAG AAAGGTGCCC 
TTGCTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Two novel loci, COBL and SLC10A2, for Alzheimer's disease in African Americans.
Alzheimer's & dementia : the journal of the Alzheimer's Association13(2)119-129(2017 Feb)
Mez J,Chung J,Jun G,Kriegel J,Bourlas AP,Sherva R,Logue MW,Barnes LL,Bennett DA,Buxbaum JD,Byrd GS,Crane PK,Ertekin-Taner N,Evans D,Fallin MD,Foroud T,Goate A,Graff-Radford NR,Hall KS,Kamboh MI,Kukull WA,Larson EB,Manly JJ,,Haines JL,Mayeux R,Pericak-Vance MA,Schellenberg GD,Lunetta KL,Farrer LA

COBL is a novel hotspot for IKZF1 deletions in childhood acute lymphoblastic leukemia.
Oncotarget7(33)53064-53073(2016 Aug)
Lopes BA,Meyer C,Barbosa TC,Zur Stadt U,Horstmann M,Venn NC,Heatley S,White DL,Sutton R,Pombo-de-Oliveira MS,Marschalek R,Emerenciano M

The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.
PLoS biology13(9)e1002233(2015)
Hou W,Izadi M,Nemitz S,Haag N,Kessels MM,Qualmann B

Mutagenetic and electron microscopy analysis of actin filament severing by Cordon-Bleu, a WH2 domain protein.
Cytoskeleton (Hoboken, N.J.)71(3)170-83(2014 Mar)
Jiao Y,Walker M,Trinick J,Pernier J,Montaville P,Carlier MF

Association of granulomatosis with polyangiitis (Wegener's) with HLA-DPB1*04 and SEMA6A gene variants: evidence from genome-wide analysis.
Arthritis and rheumatism65(9)2457-68(2013 Sep)
Xie G,Roshandel D,Sherva R,Monach PA,Lu EY,Kung T,Carrington K,Zhang SS,Pulit SL,Ripke S,Carette S,Dellaripa PF,Edberg JC,Hoffman GS,Khalidi N,Langford CA,Mahr AD,St Clair EW,Seo P,Specks U,Spiera RF,Stone JH,Ytterberg SR,Raychaudhuri S,de Bakker PI,Farrer LA,Amos CI,Merkel PA,Siminovitch KA

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COBL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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