NUP205 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP205
Entrez Gene ID	23165
Full Name	nucleoporin 205
Synonyms	C7orf14,NPHS13
General protein information	Preferred Names nucleoporin 205 Names nuclear pore complex protein Nup205 205 kDa nucleoporin nucleoporin Nup205
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q33
Summary	This gene encodes a nucleoporin, which is a subunit of the nuclear pore complex that functions in active transport of proteins, RNAs and ribonucleoprotein particles between the nucleus and cytoplasm. Mutations in this gene are associated with steroid-resistant nephrotic syndrome. [provided by RefSeq, Jul 2016].
Disorder MIM:	614352
Disorder Html:	?Nephrotic syndrome, type 13, 616893 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329434.1	NP_001316363.1	nuclear pore complex protein Nup205 isoform 2
NM_015135.3	NP_055950.2	nuclear pore complex protein Nup205 isoform 1
NM_015135.2	NP_055950.1	nuclear pore complex protein Nup205 isoform 1
NM_001329434.2	NP_001316363.2	nuclear pore complex protein Nup205 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NUP205	XP_416176.2
Arabidopsis thaliana (thale cress)	AT5G51200	NP_199933.5
Pan troglodytes (chimpanzee)	NUP205	XP_527899.3
Rattus norvegicus (Norway rat)	Nup205	NP_001102090.1
Drosophila melanogaster (fruit fly)	Nup205	NP_728308.1
	NUP205	XP_001105911.1
Bos taurus (cattle)	NUP205	NP_001124403.1
Mus musculus (house mouse)	Nup205	NP_081789.1
Danio rerio (zebrafish)	nup205	NP_001003859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008090	XP_317367.4
Xenopus tropicalis (tropical clawed frog)	nup205	NP_001135596.1
Homo sapiens (human)	NUP205	NP_055950.1
Canis lupus familiaris (dog)	NUP205	XP_005629638.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.
Braun DA, Sadowski CE, Kohl S, Lovric S, Astrinidis SA, Pabst WL, Gee HY, Ashraf S, Lawson JA, Shril S, Airik M, Tan W, Schapiro D, Rao J, Choi WI, Hermle T, Kemper MJ, Pohl M, Ozaltin F, Konrad M, Bogdanovic R, B?scher R, Helmchen U, Serdaroglu E, Lifton RP, Antonin W, Hildebrandt F
Nature genetics48(4)457-65(2016 Apr)

Interaction of adenovirus type 5 E4orf4 with the nuclear pore subunit Nup205 is required for proper viral gene expression.
Lu Y, Kucharski TJ, Gamache I, Blanchette P, Branton PE, Teodoro JG
Journal of virology88(22)13249-59(2014 Nov)

Seventy-five genetic loci influencing the human red blood cell.
van der Harst P, Zhang W, Mateo Leach I, Rendon A, Verweij N, Sehmi J, Paul DS, Elling U, Allayee H, Li X, Radhakrishnan A, Tan ST, Voss K, Weichenberger CX, Albers CA, Al-Hussani A, Asselbergs FW, Ciullo M, Danjou F, Dina C, Esko T, Evans DM, Franke L, G?gele M, Hartiala J, Hersch M, Holm H, Hottenga JJ, Kanoni S, Kleber ME, Lagou V, Langenberg C, Lopez LM, Lyytik?inen LP, Melander O, Murgia F, Nolte IM, O'Reilly PF, Padmanabhan S, Parsa A, Pirastu N, Porcu E, Portas L, Prokopenko I, Ried JS, Shin SY, Tang CS, Teumer A, Traglia M, Ulivi S, Westra HJ, Yang J, Zhao JH, Anni F, Abdellaoui A, Attwood A, Balkau B, Bandinelli S, Bastardot F, Benyamin B, Boehm BO, Cookson WO, Das D, de Bakker PI, de Boer RA, de Geus EJ, de Moor MH, Dimitriou M, Domingues FS, D?ring A, Engstr?m G, Eyjolfsson GI, Ferrucci L, Fischer K, Galanello R, Garner SF, Genser B, Gibson QD, Girotto G, Gudbjartsson DF, Harris SE, Hartikainen AL, Hastie CE, Hedblad B, Illig T, Jolley J, K?h?nen M, Kema IP, Kemp JP, Liang L, Lloyd-Jones H, Loos RJ, Meacham S, Medland SE, Meisinger C, Memari Y, Mihailov E, Miller K, Moffatt MF, Nauck M, Novatchkova M, Nutile T, Olafsson I, Onundarson PT, Parracciani D, Penninx BW, Perseu L, Piga A, Pistis G, Pouta A, Puc U, Raitakari O, Ring SM, Robino A, Ruggiero D, Ruokonen A, Saint-Pierre A, Sala C, Salumets A, Sambrook J, Schepers H, Schmidt CO, Sillj? HH, Sladek R, Smit JH, Starr JM, Stephens J, Sulem P, Tanaka T, Thorsteinsdottir U, Tragante V, van Gilst WH, van Pelt LJ, van Veldhuisen DJ, V?lker U, Whitfield JB, Willemsen G, Winkelmann BR, Wirnsberger G, Algra A, Cucca F, d'Adamo AP, Danesh J, Deary IJ, Dominiczak AF, Elliott P, Fortina P, Froguel P, Gasparini P, Greinacher A, Hazen SL, Jarvelin MR, Khaw KT, Lehtim?ki T, Maerz W, Martin NG, Metspalu A, Mitchell BD, Montgomery GW, Moore C, Navis G, Pirastu M, Pramstaller PP, Ramirez-Solis R, Schadt E, Scott J, Shuldiner AR, Smith GD, Smith JG, Snieder H, Sorice R, Spector TD, Stefansson K, Stumvoll M, Tang WH, Toniolo D, T?njes A, Visscher PM, Vollenweider P, Wareham NJ, Wolffenbuttel BH, Boomsma DI, Beckmann JS, Dedoussis GV, Deloukas P, Ferreira MA, Sanna S, Uda M, Hicks AA, Penninger JM, Gieger C, Kooner JS, Ouwehand WH, Soranzo N, Chambers JC
Nature492(7429)369-75(2012 Dec)

Genome-wide association identifies three new susceptibility loci for Paget's disease of bone.
Albagha OM, Wani SE, Visconti MR, Alonso N, Goodman K, Brandi ML, Cundy T, Chung PY, Dargie R, Devogelaer JP, Falchetti A, Fraser WD, Gennari L, Gianfrancesco F, Hooper MJ, Van Hul W, Isaia G, Nicholson GC, Nuti R, Papapoulos S, Montes Jdel P, Ratajczak T, Rea SL, Rendina D, Gonzalez-Sarmiento R, Di Stefano M, Ward LC, Walsh JP, Ralston SH,
Nature genetics43(7)685-9(2011 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NUP93 knockdown reduced the presence of NUP205 in the nuclear pore compex, and, reciprocally, a NUP205 alteration abrogated NUP93 interaction.
Title: Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.

These data show for first time that adenovirus type 5 E4orf4 interacts with and modifies the nuclear pore complex and that Nup205-E4orf4 binding is required for normal regulation of viral gene expression and viral replication.
Title: Interaction of adenovirus type 5 E4orf4 with the nuclear pore subunit Nup205 is required for proper viral gene expression.

The following NUP205 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP205 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu101481	NM_001329434.1 Latest version!	Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu41457	NM_001329434.2 Latest version!	Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu107288	NM_015135.3 Latest version!	Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu12858	NM_015135.2 Latest version!	Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP205 ( NM_001329434.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316363.1 Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101481
Clone ID Related Accession (Same CDS sequence)	NM_001329434.1
Accession Version	NM_001329434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup205 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093107.3 and BC146784.1. On Jul 12, 2016 this sequence version replaced XM_005250235.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.137816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TCGCAATTGC TGACAACGTT TTCCTGTTCC TCATGGAATC TGTAGTGGTA 
TCAGAATACT TTTATCAGGA AGAATTTTAT ATTCGCAGAG TCCATAATCT CATCACAGAT 
TTCCTTGCAC TTATGCCAAT GAAGGTGAAA CAGCTGAGGA ATCGGGCAGA TGAAGATGCT 
CGAATGATTC ACATGAGTAT GCAGATGGGT AATGAACCCC CCATTTCACT TAGAAGGGAC 
CTGGAACACT TAATGCTTTT GATTGGCGAG CTATATAAAA AGAACCCTTT TCATCTGGAG 
CTTGCTCTAG AATATTGGTG TCCCACAGAG CCTCTTCAGA CTCCGACTAT CATGGGCTCT 
TATCTAGGGG TGGCTCATCA GCGGCCCCCT CAACGCCAGG TTGTCTTGTC AAAGTTTGTT 
AGGCAAATGG GTGACCTGTT GCCTCCAACT ATTTATATTC CTTATTTGAA GATGCTCCAG 
GGATTGGCCA ATGGGCCTCA GTGTGCCCAC TACTGTTTCA GCCTGCTCAA AGTCAATGGT 
AGTAGTCATG TTGAAAATAT TCAGGGAGCA GGTGGCAGTC CTGTTTCCTG GGAACATTTC 
TTTCACTCCT TGATGCTTTA CCACGAACAC CTTCGGAAGG ATCTTCCAAG TGCAGATAGT 
GTCCAGTACC GTCACCTTCC TTCCCGTGGC ATCACCCAGA AGGAGCAAGA TGGATTGATT 
GCTTTTTTGC AGCTCACGTC TACCATCATT ACTTGGAGTG AAAATGCTCG CTTGGCACTC 
TGTGAACACC CTCAGTGGAC CCCTGTTGTG GTGATTCTGG GACTCCTCCA ATGCAGTATT 
CCCCCTGTCC TAAAAGCTGA GCTACTGAAG ACACTCGCAG CTTTTGGAAA ATCTCCTGAA 
ATTGCTGCTT CCCTCTGGCA GTCATTGGAA TACACTCAGA TACTGCAGAC CGTGAGGATT 
CCAAGCCAAA GGCAAGCTAT TGGTATTGAG GTTGAACTAA ATGAAATAGA ATCCCGGTGT 
GAAGAATACC CATTGACTCG GGCCTTTTGC CAGCTTATTA GTACTCTGGT GGAGAGCTCA 
TTTCCTTCTA ATTTGGGTGC TGGACTGCGG CCCCCTGGCT TTGACCCTTA TTTGCAGTTC 
CTTAGAGACT CTGTGTTTCT ACGATTCCGT ACAAGAGCTT ACCGGAGAGC AGCTGAAAAG 
TGGGAAGTTG CTGAGGTGGT TTTGGAGGTG TTTTATAAAT TGCTCAGAGA TTATGAGCCT 
CAGCTTGAAG ATTTTGTAGA CCAGTTTGTG GAACTACAAG GAGAAGAAAT CATAGCCTAT 
AAGCCACCTG GATTTAGTCT GATGTATCAT CTGCTGAATG AGTCACCAAT GTTGGAGCTT 
GCTCTCAGTT TACTGGAAGA AGGAGTTAAG CAGCTTGACA CCTATGCCCC CTTTCCTGGG 
AAAAAACACC TGGAGAAAGC AGTACAGCAT TGCCTTGCAC TTCTCAATCT TACTCTGCAA 
AAGGAAAATC TTTTTATGGA CCTTCTAAGA GAGAGTCAAC TGGCTCTAAT AGTCTGTCCT 
TTAGAACAGC TTTTGCAGGG AATTAATCCC AGAACTAAGA AGGCAGATAA TGTGGTAAAC 
ATTGCCAGAT ACCTATATCA TGGCAATACT AATCCAGAAT TGGCTTTTGA AAGTGCCAAG 
ATCCTCTGTT GTATCTCTTG CAACTCTAAT ATTCAGATAA AGTTGGTTGG AGATTTCACA 
CATGACCAGA GTATAAGTCA GAAACTAATG GCTGGATTTG TGGAGTGTTT GGATTGTGAA 
GATGCAGAAG AATTTGTACG TCTGGAAGAG GGATCAGAAC TTGAAAAGAA ATTAGTTGCA 
ATTCGTCATG AAACAAGAAT CCACATCTTG AATCTTCTCA TTACCTCTCT GGAATGCAAT 
CCACCCAATC TTGCTCTCTA CCTGTTGGGC TTTGAATTGA AAAAACCTGT CAGTACTACA 
AACCTACAAG ATCCAGGAGT GTTAGGTTGC CCTCGGACAT GCCTTCACGC CATTCTAAAC 
ATCTTGGAGA AAGGAACGGA AGGGAGAACA GGCCCAGTGG CGGTGCGAGA ATCTCCTCAG 
CTGGCTGAGC TATGTTACCA GGTCATATAT CAGTTATGTG CATGCTCTGA TACATCTGGT 
CCTACTATGA GGTACTTGAG AACCAGCCAG GATTTCTTAT TTTCCCAGTT GCAGTATCTA 
CCATTTTCTA ACAAAGAATA TGAAATATCT ATGCTGAACC AGATGTCATG GCTTATGAAA 
ACTGCCTCAA TAGAGCTAAG GGTAACCTCT CTGAATCGTC AGCGGTCACA TACCCAGAGG 
CTCCTACACC TCTTACTGGA TGACATGCCA GTGAAACCAT ACTCAGATGG TGAAGGAGGA 
ATAGAAGATG AAAACAGGTC TGTTTCTGGG TTCCTTCACT TTGACACTGC TACAAAAGTA 
CGTCGAAAAA TTCTAAATAT TCTTGACTCG ATTGACTTCA GTCAGGAGAT CCCTGAGCCT 
TTGCAGTTGG ATTTTTTTGA TCGGGCCCAG ATTGAACAAG TTATTGCTAA CTGTGAACAC 
AAGAATTTAC GGGGACAGAC AGTCTGCAAT GTCAAGCTTC TTCATAGGGT TCTTGTAGCT 
GAAGTAAATG CCCTTCAGGG TATGGCAGCC ATAGGACAGA GACCTCTACT AATGGAGGAA 
ATCAGCACTG TACTTCAGTA TGTGGTAGGA AGAAATAAAT TGCTGCAGTG TCTTCATGCA 
AAACGTCATG CTCTGGAGTC GTGGAGGCAA CTAGTAGAAA TTATACTGAC AGCTTGTCCC 
CAGGACCTCA TTCAGGCAGA GGATCGACAA CTGATTATTC GTGATATTTT ACAAGATGTG 
CATGATAAGA TACTGGATGA TGAAGCTGCG CAAGAGTTAA TGCCTGTGGT CGCCGGGGCA 
GTGTTCACAC TGACTGCTCA CCTAAGCCAG GCCGTCCTCA CTGAACAGAA GCAAACATCA 
GTCTTGGGAC CAGCAGAGGC CCATTACGCT TTTATGCTTG ATAGTTGCTT CACCTCACCT 
CCTCCTGAAG AGAACCCATT AGTGGGTTTT GCTTCTATTG GAGATTCTTC ACTTTACATC 
ATATTGAAGA AACTGTTAGA CTTCATTTTG AAGACAGGTG GTGGATTCCA ACGAGTGAGG 
ACTCACTTGT ATGGCTCTCT GCTTTATTAC TTACAGATTG CCCAGAGACC TGATGAACCA 
GACACCTTAG AAGCAGCCAA GAAAACCATG TGGGAAAGGC TGACAGCCCC TGAAGATGTA 
TTTAGCAAAT TACAGCGAGA AAACATAGCC ATTATTGAAA GTTATGGCGC CGCCCTCATG 
GAAGTGGTCT GTCGAGATGC TTGTGATGGT CATGAGATTG GAAGGATGCT GGCCCTGGCT 
CTACTTGATA GAATTGTCTC CGTGGATAAA CAGCAGCAGT GGCTTTTGTA TCTTTCTAAC 
AGTGGCTACT TGAAGGTCCT CGTAGACAGC TTGGTAGAAG ATGACCGTAC TTTGCAGAGC 
TTACTCACCC CACAGCCTCC CCTTTTAAAA GCACTTTATA CTTATGAATC TAAAATGGCA 
TTTCTCACAA GAGTGGCAAA GATACAGCAG GGTGCATTAG AGCTGCTAAG ATCAGGGGTG 
ATTGTGAGAC TAGCTCAATG CCAAGTCTAT GACATGCGCC CAGAAACGGA CCCGCAGAGC 
ATGTTTGGCA TGAGAGACCC TCCAATGTTC ATCCCTACCC CAGTGGATCG CTACCGCCAG 
ATTCTCCTCC CAGCTCTCCA GCTGTGCCAG GTCATCCTCA CATCTAGTAT GGCCCAGCAC 
TTGCAGGCAG CAGGGCAGGT ATTGCAGTTT CTTATTTCAC ATTCTGATAC CATACAAGCA 
ATTCTGCGCT GTCAGGATGT TAGTGCTGGG TCTTTGCAGG AATTGGCTCT GCTGACAGGA 
ATTATAAGTA AAGCAGCACT TCCTGGAATA TTAAGTGAAC TTGACGTTGA TGTAAATGAA 
GGGTCTCTAA TGGAGCTACA GGGACATATT GGAAGATTCC AGCGCCAGTG CTTAGGACTA 
CTAAGTCGCT TTGGTGGCTC TGACAGACTG CGTCAGTTTA AATTTCAAGA CGATAATGTG 
GAGGGAGATA AAGTAAGCAA GAAAGATGAG ATTGAACTGG CTATGCAGCA GATTTGTGCC 
AATGTAATGG AATATTGCCA GTCACTCATG TTACAGAGTT CCCCTACCTT CCAGCATGCT 
GTGTGTCTCT TCACTCCTAG CCTTTCAGAA ACAGTTAATA GAGATGGACC GCGGCAAGAT 
ACCCAAGCTC CAGTGGTTCC ATACTGGCGC CTGCCTGGTT TAGGCATTAT CATCTACCTG 
CTGAAACAGA GTGCTAATGA TTTCTTCAGC TATTATGACA GTCATCGACA GAGTGTCAGC 
AAGCTACAAA ATGTAGAGCA GCTTCCCCCA GATGAGATAA AAGAGTTGTG TCAGTCTGTG 
ATGCCTGCTG GTGTTGATAA AATCTCCACT GCTCAGAAAT ATGTTCTAGC AAGACGGCGC 
TTGGTGAAGG TGATCAACAA TCGAGCTAAA CTGCTTTCCC TTTGTTCTTT TATCATAGAG 
ACCTGCCTAT TTATTCTTTG GCGCCATCTG GAGTACTACT TGTTACATTG CATGCCCACG 
GATTCTCAAG ATTCCTTATT TGCCTCGAGA ACCTTGTTTA AAAGCAGAAG ACTGCAAGAT 
TCCTTCGCCT CAGAAACCAA TCTAGATTTT AGAAGTGGGC TGGCTATAGT GAGCCAACAT 
GATTTAGACC AGCTTCAGGC TGATGCAATC AACGCTTTTG GAGAATCACT ACAAAAGAAA 
CTTCTGGACA TTGAAGGATT ATATTCAAAA GTTCGATCTC GATATAGTTT CATACAGGCT 
CTTGTCAGAC GTATCCGTGG CCTCTTGAGG ATATCAAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316363.1
CDS	1117..6081
Translation	MAELAIADNVFLFLMESVVVSEYFYQEEFYIRRVHNLITDFLALMPMKVKQLRNRADEDARMIHMSMQMGNEPPISLRRDLEHLMLLIGELYKKNPFHLELALEYWCPTEPLQTPTIMGSYLGVAHQRPPQRQVVLSKFVRQMGDLLPPTIYIPYLKMLQGLANGPQCAHYCFSLLKVNGSSHVENIQGAGGSPVSWEHFFHSLMLYHEHLRKDLPSADSVQYRHLPSRGITQKEQDGLIAFLQLTSTIITWSENARLALCEHPQWTPVVVILGLLQCSIPPVLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRIPSQRQAIGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPSNLGAGLRPPGFDPYLQFLRDSVFLRFRTRAYRRAAEKWEVAEVVLEVFYKLLRDYEPQLEDFVDQFVELQGEEIIAYKPPGFSLMYHLLNESPMLELALSLLEEGVKQLDTYAPFPGKKHLEKAVQHCLALLNLTLQKENLFMDLLRESQLALIVCPLEQLLQGINPRTKKADNVVNIARYLYHGNTNPELAFESAKILCCISCNSNIQIKLVGDFTHDQSISQKLMAGFVECLDCEDAEEFVRLEEGSELEKKLVAIRHETRIHILNLLITSLECNPPNLALYLLGFELKKPVSTTNLQDPGVLGCPRTCLHAILNILEKGTEGRTGPVAVRESPQLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFSQLQYLPFSNKEYEISMLNQMSWLMKTASIELRVTSLNRQRSHTQRLLHLLLDDMPVKPYSDGEGGIEDENRSVSGFLHFDTATKVRRKILNILDSIDFSQEIPEPLQLDFFDRAQIEQVIANCEHKNLRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEISTVLQYVVGRNKLLQCLHAKRHALESWRQLVEIILTACPQDLIQAEDRQLIIRDILQDVHDKILDDEAAQELMPVVAGAVFTLTAHLSQAVLTEQKQTSVLGPAEAHYAFMLDSCFTSPPPEENPLVGFASIGDSSLYIILKKLLDFILKTGGGFQRVRTHLYGSLLYYLQIAQRPDEPDTLEAAKKTMWERLTAPEDVFSKLQRENIAIIESYGAALMEVVCRDACDGHEIGRMLALALLDRIVSVDKQQQWLLYLSNSGYLKVLVDSLVEDDRTLQSLLTPQPPLLKALYTYESKMAFLTRVAKIQQGALELLRSGVIVRLAQCQVYDMRPETDPQSMFGMRDPPMFIPTPVDRYRQILLPALQLCQVILTSSMAQHLQAAGQVLQFLISHSDTIQAILRCQDVSAGSLQELALLTGIISKAALPGILSELDVDVNEGSLMELQGHIGRFQRQCLGLLSRFGGSDRLRQFKFQDDNVEGDKVSKKDEIELAMQQICANVMEYCQSLMLQSSPTFQHAVCLFTPSLSETVNRDGPRQDTQAPVVPYWRLPGLGIIIYLLKQSANDFFSYYDSHRQSVSKLQNVEQLPPDEIKELCQSVMPAGVDKISTAQKYVLARRRLVKVINNRAKLLSLCSFIIETCLFILWRHLEYYLLHCMPTDSQDSLFASRTLFKSRRLQDSFASETNLDFRSGLAIVSQHDLDQLQADAINAFGESLQKKLLDIEGLYSKVRSRYSFIQALVRRIRGLLRISRN

Target ORF information:

RefSeq Version	NM_001329434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329434.1

ORF Insert Sequence:

ATGGCAGAAC TCGCAATTGC TGACAACGTT TTCCTGTTCC TCATGGAATC TGTAGTGGTA 
TCAGAATACT TTTATCAGGA AGAATTTTAT ATTCGCAGAG TCCATAATCT CATCACAGAT 
TTCCTTGCAC TTATGCCAAT GAAGGTGAAA CAGCTGAGGA ATCGGGCAGA TGAAGATGCT 
CGAATGATTC ACATGAGTAT GCAGATGGGT AATGAACCCC CCATTTCACT TAGAAGGGAC 
CTGGAACACT TAATGCTTTT GATTGGCGAG CTATATAAAA AGAACCCTTT TCATCTGGAG 
CTTGCTCTAG AATATTGGTG TCCCACAGAG CCTCTTCAGA CTCCGACTAT CATGGGCTCT 
TATCTAGGGG TGGCTCATCA GCGGCCCCCT CAACGCCAGG TTGTCTTGTC AAAGTTTGTT 
AGGCAAATGG GTGACCTGTT GCCTCCAACT ATTTATATTC CTTATTTGAA GATGCTCCAG 
GGATTGGCCA ATGGGCCTCA GTGTGCCCAC TACTGTTTCA GCCTGCTCAA AGTCAATGGT 
AGTAGTCATG TTGAAAATAT TCAGGGAGCA GGTGGCAGTC CTGTTTCCTG GGAACATTTC 
TTTCACTCCT TGATGCTTTA CCACGAACAC CTTCGGAAGG ATCTTCCAAG TGCAGATAGT 
GTCCAGTACC GTCACCTTCC TTCCCGTGGC ATCACCCAGA AGGAGCAAGA TGGATTGATT 
GCTTTTTTGC AGCTCACGTC TACCATCATT ACTTGGAGTG AAAATGCTCG CTTGGCACTC 
TGTGAACACC CTCAGTGGAC CCCTGTTGTG GTGATTCTGG GACTCCTCCA ATGCAGTATT 
CCCCCTGTCC TAAAAGCTGA GCTACTGAAG ACACTCGCAG CTTTTGGAAA ATCTCCTGAA 
ATTGCTGCTT CCCTCTGGCA GTCATTGGAA TACACTCAGA TACTGCAGAC CGTGAGGATT 
CCAAGCCAAA GGCAAGCTAT TGGTATTGAG GTTGAACTAA ATGAAATAGA ATCCCGGTGT 
GAAGAATACC CATTGACTCG GGCCTTTTGC CAGCTTATTA GTACTCTGGT GGAGAGCTCA 
TTTCCTTCTA ATTTGGGTGC TGGACTGCGG CCCCCTGGCT TTGACCCTTA TTTGCAGTTC 
CTTAGAGACT CTGTGTTTCT ACGATTCCGT ACAAGAGCTT ACCGGAGAGC AGCTGAAAAG 
TGGGAAGTTG CTGAGGTGGT TTTGGAGGTG TTTTATAAAT TGCTCAGAGA TTATGAGCCT 
CAGCTTGAAG ATTTTGTAGA CCAGTTTGTG GAACTACAAG GAGAAGAAAT CATAGCCTAT 
AAGCCACCTG GATTTAGTCT GATGTATCAT CTGCTGAATG AGTCACCAAT GTTGGAGCTT 
GCTCTCAGTT TACTGGAAGA AGGAGTTAAG CAGCTTGACA CCTATGCCCC CTTTCCTGGG 
AAAAAACACC TGGAGAAAGC AGTACAGCAT TGCCTTGCAC TTCTCAATCT TACTCTGCAA 
AAGGAAAATC TTTTTATGGA CCTTCTAAGA GAGAGTCAAC TGGCTCTAAT AGTCTGTCCT 
TTAGAACAGC TTTTGCAGGG AATTAATCCC AGAACTAAGA AGGCAGATAA TGTGGTAAAC 
ATTGCCAGAT ACCTATATCA TGGCAATACT AATCCAGAAT TGGCTTTTGA AAGTGCCAAG 
ATCCTCTGTT GTATCTCTTG CAACTCTAAT ATTCAGATAA AGTTGGTTGG AGATTTCACA 
CATGACCAGA GTATAAGTCA GAAACTAATG GCTGGATTTG TGGAGTGTTT GGATTGTGAA 
GATGCAGAAG AATTTGTACG TCTGGAAGAG GGATCAGAAC TTGAAAAGAA ATTAGTTGCA 
ATTCGTCATG AAACAAGAAT CCACATCTTG AATCTTCTCA TTACCTCTCT GGAATGCAAT 
CCACCCAATC TTGCTCTCTA CCTGTTGGGC TTTGAATTGA AAAAACCTGT CAGTACTACA 
AACCTACAAG ATCCAGGAGT GTTAGGTTGC CCTCGGACAT GCCTTCACGC CATTCTAAAC 
ATCTTGGAGA AAGGAACGGA AGGGAGAACA GGCCCAGTGG CGGTGCGAGA ATCTCCTCAG 
CTGGCTGAGC TATGTTACCA GGTCATATAT CAGTTATGTG CATGCTCTGA TACATCTGGT 
CCTACTATGA GGTACTTGAG AACCAGCCAG GATTTCTTAT TTTCCCAGTT GCAGTATCTA 
CCATTTTCTA ACAAAGAATA TGAAATATCT ATGCTGAACC AGATGTCATG GCTTATGAAA 
ACTGCCTCAA TAGAGCTAAG GGTAACCTCT CTGAATCGTC AGCGGTCACA TACCCAGAGG 
CTCCTACACC TCTTACTGGA TGACATGCCA GTGAAACCAT ACTCAGATGG TGAAGGAGGA 
ATAGAAGATG AAAACAGGTC TGTTTCTGGG TTCCTTCACT TTGACACTGC TACAAAAGTA 
CGTCGAAAAA TTCTAAATAT TCTTGACTCG ATTGACTTCA GTCAGGAGAT CCCTGAGCCT 
TTGCAGTTGG ATTTTTTTGA TCGGGCCCAG ATTGAACAAG TTATTGCTAA CTGTGAACAC 
AAGAATTTAC GGGGACAGAC AGTCTGCAAT GTCAAGCTTC TTCATAGGGT TCTTGTAGCT 
GAAGTAAATG CCCTTCAGGG TATGGCAGCC ATAGGACAGA GACCTCTACT AATGGAGGAA 
ATCAGCACTG TACTTCAGTA TGTGGTAGGA AGAAATAAAT TGCTGCAGTG TCTTCATGCA 
AAACGTCATG CTCTGGAGTC GTGGAGGCAA CTAGTAGAAA TTATACTGAC AGCTTGTCCC 
CAGGACCTCA TTCAGGCAGA GGATCGACAA CTGATTATTC GTGATATTTT ACAAGATGTG 
CATGATAAGA TACTGGATGA TGAAGCTGCG CAAGAGTTAA TGCCTGTGGT CGCCGGGGCA 
GTGTTCACAC TGACTGCTCA CCTAAGCCAG GCCGTCCTCA CTGAACAGAA GCAAACATCA 
GTCTTGGGAC CAGCAGAGGC CCATTACGCT TTTATGCTTG ATAGTTGCTT CACCTCACCT 
CCTCCTGAAG AGAACCCATT AGTGGGTTTT GCTTCTATTG GAGATTCTTC ACTTTACATC 
ATATTGAAGA AACTGTTAGA CTTCATTTTG AAGACAGGTG GTGGATTCCA ACGAGTGAGG 
ACTCACTTGT ATGGCTCTCT GCTTTATTAC TTACAGATTG CCCAGAGACC TGATGAACCA 
GACACCTTAG AAGCAGCCAA GAAAACCATG TGGGAAAGGC TGACAGCCCC TGAAGATGTA 
TTTAGCAAAT TACAGCGAGA AAACATAGCC ATTATTGAAA GTTATGGCGC CGCCCTCATG 
GAAGTGGTCT GTCGAGATGC TTGTGATGGT CATGAGATTG GAAGGATGCT GGCCCTGGCT 
CTACTTGATA GAATTGTCTC CGTGGATAAA CAGCAGCAGT GGCTTTTGTA TCTTTCTAAC 
AGTGGCTACT TGAAGGTCCT CGTAGACAGC TTGGTAGAAG ATGACCGTAC TTTGCAGAGC 
TTACTCACCC CACAGCCTCC CCTTTTAAAA GCACTTTATA CTTATGAATC TAAAATGGCA 
TTTCTCACAA GAGTGGCAAA GATACAGCAG GGTGCATTAG AGCTGCTAAG ATCAGGGGTG 
ATTGTGAGAC TAGCTCAATG CCAAGTCTAT GACATGCGCC CAGAAACGGA CCCGCAGAGC 
ATGTTTGGCA TGAGAGACCC TCCAATGTTC ATCCCTACCC CAGTGGATCG CTACCGCCAG 
ATTCTCCTCC CAGCTCTCCA GCTGTGCCAG GTCATCCTCA CATCTAGTAT GGCCCAGCAC 
TTGCAGGCAG CAGGGCAGGT ATTGCAGTTT CTTATTTCAC ATTCTGATAC CATACAAGCA 
ATTCTGCGCT GTCAGGATGT TAGTGCTGGG TCTTTGCAGG AATTGGCTCT GCTGACAGGA 
ATTATAAGTA AAGCAGCACT TCCTGGAATA TTAAGTGAAC TTGACGTTGA TGTAAATGAA 
GGGTCTCTAA TGGAGCTACA GGGACATATT GGAAGATTCC AGCGCCAGTG CTTAGGACTA 
CTAAGTCGCT TTGGTGGCTC TGACAGACTG CGTCAGTTTA AATTTCAAGA CGATAATGTG 
GAGGGAGATA AAGTAAGCAA GAAAGATGAG ATTGAACTGG CTATGCAGCA GATTTGTGCC 
AATGTAATGG AATATTGCCA GTCACTCATG TTACAGAGTT CCCCTACCTT CCAGCATGCT 
GTGTGTCTCT TCACTCCTAG CCTTTCAGAA ACAGTTAATA GAGATGGACC GCGGCAAGAT 
ACCCAAGCTC CAGTGGTTCC ATACTGGCGC CTGCCTGGTT TAGGCATTAT CATCTACCTG 
CTGAAACAGA GTGCTAATGA TTTCTTCAGC TATTATGACA GTCATCGACA GAGTGTCAGC 
AAGCTACAAA ATGTAGAGCA GCTTCCCCCA GATGAGATAA AAGAGTTGTG TCAGTCTGTG 
ATGCCTGCTG GTGTTGATAA AATCTCCACT GCTCAGAAAT ATGTTCTAGC AAGACGGCGC 
TTGGTGAAGG TGATCAACAA TCGAGCTAAA CTGCTTTCCC TTTGTTCTTT TATCATAGAG 
ACCTGCCTAT TTATTCTTTG GCGCCATCTG GAGTACTACT TGTTACATTG CATGCCCACG 
GATTCTCAAG ATTCCTTATT TGCCTCGAGA ACCTTGTTTA AAAGCAGAAG ACTGCAAGAT 
TCCTTCGCCT CAGAAACCAA TCTAGATTTT AGAAGTGGGC TGGCTATAGT GAGCCAACAT 
GATTTAGACC AGCTTCAGGC TGATGCAATC AACGCTTTTG GAGAATCACT ACAAAAGAAA 
CTTCTGGACA TTGAAGGATT ATATTCAAAA GTTCGATCTC GATATAGTTT CATACAGGCT 
CTTGTCAGAC GTATCCGTGG CCTCTTGAGG ATATCAAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP205 ( NM_001329434.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316363.2 Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.

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CloneID	OHu41457
Clone ID Related Accession (Same CDS sequence)	NM_001329434.2
Accession Version	NM_001329434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup205 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093107.3 and KF458614.1. On May 31, 2019 this sequence version replaced NM_001329434.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.137816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TCGCAATTGC TGACAACGTT TTCCTGTTCC TCATGGAATC TGTAGTGGTA 
TCAGAATACT TTTATCAGGA AGAATTTTAT ATTCGCAGAG TCCATAATCT CATCACAGAT 
TTCCTTGCAC TTATGCCAAT GAAGGTGAAA CAGCTGAGGA ATCGGGCAGA TGAAGATGCT 
CGAATGATTC ACATGAGTAT GCAGATGGGT AATGAACCCC CCATTTCACT TAGAAGGGAC 
CTGGAACACT TAATGCTTTT GATTGGCGAG CTATATAAAA AGAACCCTTT TCATCTGGAG 
CTTGCTCTAG AATATTGGTG TCCCACAGAG CCTCTTCAGA CTCCGACTAT CATGGGCTCT 
TATCTAGGGG TGGCTCATCA GCGGCCCCCT CAACGCCAGG TTGTCTTGTC AAAGTTTGTT 
AGGCAAATGG GTGACCTGTT GCCTCCAACT ATTTATATTC CTTATTTGAA GATGCTCCAG 
GGATTGGCCA ATGGGCCTCA GTGTGCCCAC TACTGTTTCA GCCTGCTCAA AGTCAATGGT 
AGTAGTCATG TTGAAAATAT TCAGGGAGCA GGTGGCAGTC CTGTTTCCTG GGAACATTTC 
TTTCACTCCT TGATGCTTTA CCACGAACAC CTTCGGAAGG ATCTTCCAAG TGCAGATAGT 
GTCCAGTACC GTCACCTTCC TTCCCGTGGC ATCACCCAGA AGGAGCAAGA TGGATTGATT 
GCTTTTTTGC AGCTCACGTC TACCATCATT ACTTGGAGTG AAAATGCTCG CTTGGCACTC 
TGTGAACACC CTCAGTGGAC CCCTGTTGTG GTGATTCTGG GACTCCTCCA ATGCAGTATT 
CCCCCTGTCC TAAAAGCTGA GCTACTGAAG ACACTCGCAG CTTTTGGAAA ATCTCCTGAA 
ATTGCTGCTT CCCTCTGGCA GTCATTGGAA TACACTCAGA TACTGCAGAC CGTGAGGATT 
CCAAGCCAAA GGCAAGCTAT TGGTATTGAG GTTGAACTAA ATGAAATAGA ATCCCGGTGT 
GAAGAATACC CATTGACTCG GGCCTTTTGC CAGCTTATTA GTACTCTGGT GGAGAGCTCA 
TTTCCTTCTA ATTTGGGTGC TGGACTGCGG CCCCCTGGCT TTGACCCTTA TTTGCAGTTC 
CTTAGAGACT CTGTGTTTCT ACGATTCCGT ACAAGAGCTT ACCGGAGAGC AGCTGAAAAG 
TGGGAAGTTG CTGAGGTGGT TTTGGAGGTG TTTTATAAAT TGCTCAGAGA TTATGAGCCT 
CAGCTTGAAG ATTTTGTAGA CCAGTTTGTG GAACTACAAG GAGAAGAAAT CATAGCCTAT 
AAGCCACCTG GATTTAGTCT GATGTATCAT CTGCTGAATG AGTCACCAAT GTTGGAGCTT 
GCTCTCAGTT TACTGGAAGA AGGAGTTAAG CAGCTTGACA CCTATGCCCC CTTTCCTGGG 
AAAAAACACC TGGAGAAAGC AGTACAGCAT TGCCTTGCAC TTCTCAATCT TACTCTGCAA 
AAGGAAAATC TTTTTATGGA CCTTCTAAGA GAGAGTCAAC TGGCTCTAAT AGTCTGTCCT 
TTAGAACAGC TTTTGCAGGG AATTAATCCC AGAACTAAGA AGGCAGATAA TGTGGTAAAC 
ATTGCCAGAT ACCTATATCA TGGCAATACT AATCCAGAAT TGGCTTTTGA AAGTGCCAAG 
ATCCTCTGTT GTATCTCTTG CAACTCTAAT ATTCAGATAA AGTTGGTTGG AGATTTCACA 
CATGACCAGA GTATAAGTCA GAAACTAATG GCTGGATTTG TGGAGTGTTT GGATTGTGAA 
GATGCAGAAG AATTTGTACG TCTGGAAGAG GGATCAGAAC TTGAAAAGAA ATTAGTTGCA 
ATTCGTCATG AAACAAGAAT CCACATCTTG AATCTTCTCA TTACCTCTCT GGAATGCAAT 
CCACCCAATC TTGCTCTCTA CCTGTTGGGC TTTGAATTGA AAAAACCTGT CAGTACTACA 
AACCTACAAG ATCCAGGAGT GTTAGGTTGC CCTCGGACAT GCCTTCACGC CATTCTAAAC 
ATCTTGGAGA AAGGAACGGA AGGGAGAACA GGCCCAGTGG CGGTGCGAGA ATCTCCTCAG 
CTGGCTGAGC TATGTTACCA GGTCATATAT CAGTTATGTG CATGCTCTGA TACATCTGGT 
CCTACTATGA GGTACTTGAG AACCAGCCAG GATTTCTTAT TTTCCCAGTT GCAGTATCTA 
CCATTTTCTA ACAAAGAATA TGAAATATCT ATGCTGAACC AGATGTCATG GCTTATGAAA 
ACTGCCTCAA TAGAGCTAAG GGTAACCTCT CTGAATCGTC AGCGGTCACA TACCCAGAGG 
CTCCTACACC TCTTACTGGA TGACATGCCA GTGAAACCAT ACTCAGATGG TGAAGGAGGA 
ATAGAAGATG AAAACAGGTC TGTTTCTGGG TTCCTTCACT TTGACACTGC TACAAAAGTA 
CGTCGAAAAA TTCTAAATAT TCTTGACTCG ATTGACTTCA GTCAGGAGAT CCCTGAGCCT 
TTGCAGTTGG ATTTTTTTGA TCGGGCCCAG ATTGAACAAG TTATTGCTAA CTGTGAACAC 
AAGAATTTAC GGGGACAGAC AGTCTGCAAT GTCAAGCTTC TTCATAGGGT TCTTGTAGCT 
GAAGTAAATG CCCTTCAGGG TATGGCAGCC ATAGGACAGA GACCTCTACT AATGGAGGAA 
ATCAGCACTG TACTTCAGTA TGTGGTAGGA AGAAATAAAT TGCTGCAGTG TCTTCATGCA 
AAACGTCATG CTCTGGAGTC GTGGAGGCAA CTAGTAGAAA TTATACTGAC AGCTTGTCCC 
CAGGACCTCA TTCAGGCAGA GGATCGACAA CTGATTATTC GTGATATTTT ACAAGATGTG 
CATGATAAGA TACTGGATGA TGAAGCTGCG CAAGAGTTAA TGCCTGTGGT CGCCGGGGCA 
GTGTTCACAC TGACTGCTCA CCTAAGCCAG GCCGTCCTCA CTGAACAGAA GGAAACATCA 
GTCTTGGGAC CAGCAGAGGC CCATTACGCT TTTATGCTTG ATAGTTGCTT CACCTCACCT 
CCTCCTGAAG AGAACCCATT AGTGGGTTTT GCTTCTATTG GAGATTCTTC ACTTTACATC 
ATATTGAAGA AACTGTTAGA CTTCATTTTG AAGACAGGTG GTGGATTCCA ACGAGTGAGG 
ACTCACTTGT ATGGCTCTCT GCTTTATTAC TTACAGATTG CCCAGAGACC TGATGAACCA 
GACACCTTAG AAGCAGCCAA GAAAACCATG TGGGAAAGGC TGACAGCCCC TGAAGATGTA 
TTTAGCAAAT TACAGCGAGA AAACATAGCC ATTATTGAAA GTTATGGCGC CGCCCTCATG 
GAAGTGGTCT GTCGAGATGC TTGTGATGGT CATGAGATTG GAAGGATGCT GGCCCTGGCT 
CTACTTGATA GAATTGTCTC CGTGGATAAA CAGCAGCAGT GGCTTTTGTA TCTTTCTAAC 
AGTGGCTACT TGAAGGTCCT CGTAGACAGC TTGGTAGAAG ATGACCGTAC TTTGCAGAGC 
TTACTCACCC CACAGCCTCC CCTTTTAAAA GCACTTTATA CTTATGAATC TAAAATGGCA 
TTTCTCACAA GAGTGGCAAA GATACAGCAG GGTGCATTAG AGCTGCTAAG ATCAGGGGTG 
ATTGTGAGAC TAGCTCAATG CCAAGTCTAT GACATGCGCC CAGAAACGGA CCCGCAGAGC 
ATGTTTGGCA TGAGAGACCC TCCAATGTTC ATCCCTACCC CAGTGGATCG CTACCGCCAG 
ATTCTCCTCC CAGCTCTCCA GCTGTGCCAG GTCATCCTCA CATCTAGTAT GGCCCAGCAC 
TTGCAGGCAG CAGGGCAGGT ATTGCAGTTT CTTATTTCAC ATTCTGATAC CATACAAGCA 
ATTCTGCGCT GTCAGGATGT TAGTGCTGGG TCTTTGCAGG AATTGGCTCT GCTGACAGGA 
ATTATAAGTA AAGCAGCACT TCCTGGAATA TTAAGTGAAC TTGACGTTGA TGTAAATGAA 
GGGTCTCTAA TGGAGCTACA GGGACATATT GGAAGATTCC AGCGCCAGTG CTTAGGACTA 
CTAAGTCGCT TTGGTGGCTC TGACAGACTG CGTCAGTTTA AATTTCAAGA CGATAATGTG 
GAGGGAGATA AAGTAAGCAA GAAAGATGAG ATTGAACTGG CTATGCAGCA GATTTGTGCC 
AATGTAATGG AATATTGCCA GTCACTCATG TTACAGAGTT CCCCTACCTT CCAGCATGCT 
GTGTGTCTCT TCACTCCTAG CCTTTCAGAA ACAGTTAATA GAGATGGACC GCGGCAAGAT 
ACCCAAGCTC CAGTGGTTCC ATACTGGCGC CTGCCTGGTT TAGGCATTAT CATCTACCTG 
CTGAAACAGA GTGCTAATGA TTTCTTCAGC TATTATGACA GTCATCGACA GAGTGTCAGC 
AAGCTACAAA ATGTAGAGCA GCTTCCCCCA GATGAGATAA AAGAGTTGTG TCAGTCTGTG 
ATGCCTGCTG GTGTTGATAA AATCTCCACT GCTCAGAAAT ATGTTCTAGC AAGACGGCGC 
TTGGTGAAGG TGATCAACAA TCGAGCTAAA CTGCTTTCCC TTTGTTCTTT TATCATAGAG 
ACCTGCCTAT TTATTCTTTG GCGCCATCTG GAGTACTACT TGTTACATTG CATGCCCACG 
GATTCTCAAG ATTCCTTATT TGCCTCGAGA ACCTTGTTTA AAAGCAGAAG ACTGCAAGAT 
TCCTTCGCCT CAGAAACCAA TCTAGATTTT AGAAGTGGGC TGGCTATAGT GAGCCAACAT 
GATTTAGACC AGCTTCAGGC TGATGCAATC AACGCTTTTG GAGAATCACT ACAAAAGAAA 
CTTCTGGACA TTGAAGGATT ATATTCAAAA GTTCGATCTC GATATAGTTT CATACAGGCT 
CTTGTCAGAC GTATCCGTGG CCTCTTGAGG ATATCAAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316363.2
CDS	1114..6078
Translation	MAELAIADNVFLFLMESVVVSEYFYQEEFYIRRVHNLITDFLALMPMKVKQLRNRADEDARMIHMSMQMGNEPPISLRRDLEHLMLLIGELYKKNPFHLELALEYWCPTEPLQTPTIMGSYLGVAHQRPPQRQVVLSKFVRQMGDLLPPTIYIPYLKMLQGLANGPQCAHYCFSLLKVNGSSHVENIQGAGGSPVSWEHFFHSLMLYHEHLRKDLPSADSVQYRHLPSRGITQKEQDGLIAFLQLTSTIITWSENARLALCEHPQWTPVVVILGLLQCSIPPVLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRIPSQRQAIGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPSNLGAGLRPPGFDPYLQFLRDSVFLRFRTRAYRRAAEKWEVAEVVLEVFYKLLRDYEPQLEDFVDQFVELQGEEIIAYKPPGFSLMYHLLNESPMLELALSLLEEGVKQLDTYAPFPGKKHLEKAVQHCLALLNLTLQKENLFMDLLRESQLALIVCPLEQLLQGINPRTKKADNVVNIARYLYHGNTNPELAFESAKILCCISCNSNIQIKLVGDFTHDQSISQKLMAGFVECLDCEDAEEFVRLEEGSELEKKLVAIRHETRIHILNLLITSLECNPPNLALYLLGFELKKPVSTTNLQDPGVLGCPRTCLHAILNILEKGTEGRTGPVAVRESPQLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFSQLQYLPFSNKEYEISMLNQMSWLMKTASIELRVTSLNRQRSHTQRLLHLLLDDMPVKPYSDGEGGIEDENRSVSGFLHFDTATKVRRKILNILDSIDFSQEIPEPLQLDFFDRAQIEQVIANCEHKNLRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEISTVLQYVVGRNKLLQCLHAKRHALESWRQLVEIILTACPQDLIQAEDRQLIIRDILQDVHDKILDDEAAQELMPVVAGAVFTLTAHLSQAVLTEQKETSVLGPAEAHYAFMLDSCFTSPPPEENPLVGFASIGDSSLYIILKKLLDFILKTGGGFQRVRTHLYGSLLYYLQIAQRPDEPDTLEAAKKTMWERLTAPEDVFSKLQRENIAIIESYGAALMEVVCRDACDGHEIGRMLALALLDRIVSVDKQQQWLLYLSNSGYLKVLVDSLVEDDRTLQSLLTPQPPLLKALYTYESKMAFLTRVAKIQQGALELLRSGVIVRLAQCQVYDMRPETDPQSMFGMRDPPMFIPTPVDRYRQILLPALQLCQVILTSSMAQHLQAAGQVLQFLISHSDTIQAILRCQDVSAGSLQELALLTGIISKAALPGILSELDVDVNEGSLMELQGHIGRFQRQCLGLLSRFGGSDRLRQFKFQDDNVEGDKVSKKDEIELAMQQICANVMEYCQSLMLQSSPTFQHAVCLFTPSLSETVNRDGPRQDTQAPVVPYWRLPGLGIIIYLLKQSANDFFSYYDSHRQSVSKLQNVEQLPPDEIKELCQSVMPAGVDKISTAQKYVLARRRLVKVINNRAKLLSLCSFIIETCLFILWRHLEYYLLHCMPTDSQDSLFASRTLFKSRRLQDSFASETNLDFRSGLAIVSQHDLDQLQADAINAFGESLQKKLLDIEGLYSKVRSRYSFIQALVRRIRGLLRISRN

Target ORF information:

RefSeq Version	NM_001329434.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 205 (NUP205), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329434.2

ORF Insert Sequence:

ATGGCAGAAC TCGCAATTGC TGACAACGTT TTCCTGTTCC TCATGGAATC TGTAGTGGTA 
TCAGAATACT TTTATCAGGA AGAATTTTAT ATTCGCAGAG TCCATAATCT CATCACAGAT 
TTCCTTGCAC TTATGCCAAT GAAGGTGAAA CAGCTGAGGA ATCGGGCAGA TGAAGATGCT 
CGAATGATTC ACATGAGTAT GCAGATGGGT AATGAACCCC CCATTTCACT TAGAAGGGAC 
CTGGAACACT TAATGCTTTT GATTGGCGAG CTATATAAAA AGAACCCTTT TCATCTGGAG 
CTTGCTCTAG AATATTGGTG TCCCACAGAG CCTCTTCAGA CTCCGACTAT CATGGGCTCT 
TATCTAGGGG TGGCTCATCA GCGGCCCCCT CAACGCCAGG TTGTCTTGTC AAAGTTTGTT 
AGGCAAATGG GTGACCTGTT GCCTCCAACT ATTTATATTC CTTATTTGAA GATGCTCCAG 
GGATTGGCCA ATGGGCCTCA GTGTGCCCAC TACTGTTTCA GCCTGCTCAA AGTCAATGGT 
AGTAGTCATG TTGAAAATAT TCAGGGAGCA GGTGGCAGTC CTGTTTCCTG GGAACATTTC 
TTTCACTCCT TGATGCTTTA CCACGAACAC CTTCGGAAGG ATCTTCCAAG TGCAGATAGT 
GTCCAGTACC GTCACCTTCC TTCCCGTGGC ATCACCCAGA AGGAGCAAGA TGGATTGATT 
GCTTTTTTGC AGCTCACGTC TACCATCATT ACTTGGAGTG AAAATGCTCG CTTGGCACTC 
TGTGAACACC CTCAGTGGAC CCCTGTTGTG GTGATTCTGG GACTCCTCCA ATGCAGTATT 
CCCCCTGTCC TAAAAGCTGA GCTACTGAAG ACACTCGCAG CTTTTGGAAA ATCTCCTGAA 
ATTGCTGCTT CCCTCTGGCA GTCATTGGAA TACACTCAGA TACTGCAGAC CGTGAGGATT 
CCAAGCCAAA GGCAAGCTAT TGGTATTGAG GTTGAACTAA ATGAAATAGA ATCCCGGTGT 
GAAGAATACC CATTGACTCG GGCCTTTTGC CAGCTTATTA GTACTCTGGT GGAGAGCTCA 
TTTCCTTCTA ATTTGGGTGC TGGACTGCGG CCCCCTGGCT TTGACCCTTA TTTGCAGTTC 
CTTAGAGACT CTGTGTTTCT ACGATTCCGT ACAAGAGCTT ACCGGAGAGC AGCTGAAAAG 
TGGGAAGTTG CTGAGGTGGT TTTGGAGGTG TTTTATAAAT TGCTCAGAGA TTATGAGCCT 
CAGCTTGAAG ATTTTGTAGA CCAGTTTGTG GAACTACAAG GAGAAGAAAT CATAGCCTAT 
AAGCCACCTG GATTTAGTCT GATGTATCAT CTGCTGAATG AGTCACCAAT GTTGGAGCTT 
GCTCTCAGTT TACTGGAAGA AGGAGTTAAG CAGCTTGACA CCTATGCCCC CTTTCCTGGG 
AAAAAACACC TGGAGAAAGC AGTACAGCAT TGCCTTGCAC TTCTCAATCT TACTCTGCAA 
AAGGAAAATC TTTTTATGGA CCTTCTAAGA GAGAGTCAAC TGGCTCTAAT AGTCTGTCCT 
TTAGAACAGC TTTTGCAGGG AATTAATCCC AGAACTAAGA AGGCAGATAA TGTGGTAAAC 
ATTGCCAGAT ACCTATATCA TGGCAATACT AATCCAGAAT TGGCTTTTGA AAGTGCCAAG 
ATCCTCTGTT GTATCTCTTG CAACTCTAAT ATTCAGATAA AGTTGGTTGG AGATTTCACA 
CATGACCAGA GTATAAGTCA GAAACTAATG GCTGGATTTG TGGAGTGTTT GGATTGTGAA 
GATGCAGAAG AATTTGTACG TCTGGAAGAG GGATCAGAAC TTGAAAAGAA ATTAGTTGCA 
ATTCGTCATG AAACAAGAAT CCACATCTTG AATCTTCTCA TTACCTCTCT GGAATGCAAT 
CCACCCAATC TTGCTCTCTA CCTGTTGGGC TTTGAATTGA AAAAACCTGT CAGTACTACA 
AACCTACAAG ATCCAGGAGT GTTAGGTTGC CCTCGGACAT GCCTTCACGC CATTCTAAAC 
ATCTTGGAGA AAGGAACGGA AGGGAGAACA GGCCCAGTGG CGGTGCGAGA ATCTCCTCAG 
CTGGCTGAGC TATGTTACCA GGTCATATAT CAGTTATGTG CATGCTCTGA TACATCTGGT 
CCTACTATGA GGTACTTGAG AACCAGCCAG GATTTCTTAT TTTCCCAGTT GCAGTATCTA 
CCATTTTCTA ACAAAGAATA TGAAATATCT ATGCTGAACC AGATGTCATG GCTTATGAAA 
ACTGCCTCAA TAGAGCTAAG GGTAACCTCT CTGAATCGTC AGCGGTCACA TACCCAGAGG 
CTCCTACACC TCTTACTGGA TGACATGCCA GTGAAACCAT ACTCAGATGG TGAAGGAGGA 
ATAGAAGATG AAAACAGGTC TGTTTCTGGG TTCCTTCACT TTGACACTGC TACAAAAGTA 
CGTCGAAAAA TTCTAAATAT TCTTGACTCG ATTGACTTCA GTCAGGAGAT CCCTGAGCCT 
TTGCAGTTGG ATTTTTTTGA TCGGGCCCAG ATTGAACAAG TTATTGCTAA CTGTGAACAC 
AAGAATTTAC GGGGACAGAC AGTCTGCAAT GTCAAGCTTC TTCATAGGGT TCTTGTAGCT 
GAAGTAAATG CCCTTCAGGG TATGGCAGCC ATAGGACAGA GACCTCTACT AATGGAGGAA 
ATCAGCACTG TACTTCAGTA TGTGGTAGGA AGAAATAAAT TGCTGCAGTG TCTTCATGCA 
AAACGTCATG CTCTGGAGTC GTGGAGGCAA CTAGTAGAAA TTATACTGAC AGCTTGTCCC 
CAGGACCTCA TTCAGGCAGA GGATCGACAA CTGATTATTC GTGATATTTT ACAAGATGTG 
CATGATAAGA TACTGGATGA TGAAGCTGCG CAAGAGTTAA TGCCTGTGGT CGCCGGGGCA 
GTGTTCACAC TGACTGCTCA CCTAAGCCAG GCCGTCCTCA CTGAACAGAA GGAAACATCA 
GTCTTGGGAC CAGCAGAGGC CCATTACGCT TTTATGCTTG ATAGTTGCTT CACCTCACCT 
CCTCCTGAAG AGAACCCATT AGTGGGTTTT GCTTCTATTG GAGATTCTTC ACTTTACATC 
ATATTGAAGA AACTGTTAGA CTTCATTTTG AAGACAGGTG GTGGATTCCA ACGAGTGAGG 
ACTCACTTGT ATGGCTCTCT GCTTTATTAC TTACAGATTG CCCAGAGACC TGATGAACCA 
GACACCTTAG AAGCAGCCAA GAAAACCATG TGGGAAAGGC TGACAGCCCC TGAAGATGTA 
TTTAGCAAAT TACAGCGAGA AAACATAGCC ATTATTGAAA GTTATGGCGC CGCCCTCATG 
GAAGTGGTCT GTCGAGATGC TTGTGATGGT CATGAGATTG GAAGGATGCT GGCCCTGGCT 
CTACTTGATA GAATTGTCTC CGTGGATAAA CAGCAGCAGT GGCTTTTGTA TCTTTCTAAC 
AGTGGCTACT TGAAGGTCCT CGTAGACAGC TTGGTAGAAG ATGACCGTAC TTTGCAGAGC 
TTACTCACCC CACAGCCTCC CCTTTTAAAA GCACTTTATA CTTATGAATC TAAAATGGCA 
TTTCTCACAA GAGTGGCAAA GATACAGCAG GGTGCATTAG AGCTGCTAAG ATCAGGGGTG 
ATTGTGAGAC TAGCTCAATG CCAAGTCTAT GACATGCGCC CAGAAACGGA CCCGCAGAGC 
ATGTTTGGCA TGAGAGACCC TCCAATGTTC ATCCCTACCC CAGTGGATCG CTACCGCCAG 
ATTCTCCTCC CAGCTCTCCA GCTGTGCCAG GTCATCCTCA CATCTAGTAT GGCCCAGCAC 
TTGCAGGCAG CAGGGCAGGT ATTGCAGTTT CTTATTTCAC ATTCTGATAC CATACAAGCA 
ATTCTGCGCT GTCAGGATGT TAGTGCTGGG TCTTTGCAGG AATTGGCTCT GCTGACAGGA 
ATTATAAGTA AAGCAGCACT TCCTGGAATA TTAAGTGAAC TTGACGTTGA TGTAAATGAA 
GGGTCTCTAA TGGAGCTACA GGGACATATT GGAAGATTCC AGCGCCAGTG CTTAGGACTA 
CTAAGTCGCT TTGGTGGCTC TGACAGACTG CGTCAGTTTA AATTTCAAGA CGATAATGTG 
GAGGGAGATA AAGTAAGCAA GAAAGATGAG ATTGAACTGG CTATGCAGCA GATTTGTGCC 
AATGTAATGG AATATTGCCA GTCACTCATG TTACAGAGTT CCCCTACCTT CCAGCATGCT 
GTGTGTCTCT TCACTCCTAG CCTTTCAGAA ACAGTTAATA GAGATGGACC GCGGCAAGAT 
ACCCAAGCTC CAGTGGTTCC ATACTGGCGC CTGCCTGGTT TAGGCATTAT CATCTACCTG 
CTGAAACAGA GTGCTAATGA TTTCTTCAGC TATTATGACA GTCATCGACA GAGTGTCAGC 
AAGCTACAAA ATGTAGAGCA GCTTCCCCCA GATGAGATAA AAGAGTTGTG TCAGTCTGTG 
ATGCCTGCTG GTGTTGATAA AATCTCCACT GCTCAGAAAT ATGTTCTAGC AAGACGGCGC 
TTGGTGAAGG TGATCAACAA TCGAGCTAAA CTGCTTTCCC TTTGTTCTTT TATCATAGAG 
ACCTGCCTAT TTATTCTTTG GCGCCATCTG GAGTACTACT TGTTACATTG CATGCCCACG 
GATTCTCAAG ATTCCTTATT TGCCTCGAGA ACCTTGTTTA AAAGCAGAAG ACTGCAAGAT 
TCCTTCGCCT CAGAAACCAA TCTAGATTTT AGAAGTGGGC TGGCTATAGT GAGCCAACAT 
GATTTAGACC AGCTTCAGGC TGATGCAATC AACGCTTTTG GAGAATCACT ACAAAAGAAA 
CTTCTGGACA TTGAAGGATT ATATTCAAAA GTTCGATCTC GATATAGTTT CATACAGGCT 
CTTGTCAGAC GTATCCGTGG CCTCTTGAGG ATATCAAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP205 ( NM_015135.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055950.2 Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.

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CloneID	OHu107288
Clone ID Related Accession (Same CDS sequence)	NM_015135.3
Accession Version	NM_015135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup205 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093107.3 and KF458614.1. On Nov 23, 2018 this sequence version replaced NM_015135.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.51997.1, D86978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285968.11/ ENSP00000285968.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACGC CTTTGGCGGT AAATTCGGCT GCTAGTCTAT GGGGTCCTTA CAAAGACATT 
TGGCATAAAG TGGGAAATGC TCTTTGGAGA AGACAACCTG AAGCTGTTCA CCTTCTTGAT 
AAGATTTTGA AGAAACACAA ACCTGACTTC ATCTCATTGT TCAAAAATCC GCCAAAAAAT 
GTTCAACAGC ATGAGAAGGT TCAGAAAGCC AGTACAGAGG GAGTCGCCAT TCAGGGTCAA 
CAGGGAACTC GACTTCTTCC TGAACAGCTC ATTAAAGAAG CCTTTATTCT CAGTGACCTT 
TTTGATATTG GAGAATTGGC AGCTGTTGAG CTTCTTCTTG CTGGAGAGCA TCAACAGCCA 
CATTTTCCTG GCCTTACCAG AGGATTAGTA GCTGTTCTTC TGTACTGGGA TGGAAAGCGA 
TGCATTGCGA ATTCCTTGAA AGCCTTGATA CAGTCTAGAC GGGGAAAGAC ATGGACCCTA 
GAACTCAGTC CAGAGCTGGC TTCCATGACA ACACGCTTTA CAGATGAGCT GATGGAGCAA 
GGATTGACTT ATAAAGTTCT TACGCTGGTG TCACAGATTG ATGTGAATAA TGAGTTTGAG 
AAACTACAGC GAGAGAGAGG TTTGGGCAGT GAAAAACATC GCAAAGAGGT TTCTGATCTC 
ATTAAGGAGT GCAGGCAGTC TCTTGCAGAA AGCCTTTTTG CCTGGGCTTG CCAGTCACCT 
TTAGGCAAAG AAGACACTCT CCTCCTCATT GGACATTTGG AAAGAGTGAC AGTTGAGGCT 
AATGGCTCAC TGGATGCAGT GAATCTGGCT CTTCTTATGG CGCTTCTATA CTGTTTTGAT 
ATCAGTTTTA TAGAGCAAAG CACAGAGGAA CGAGATGATA TGATTCATCA ACTTCCACTG 
TTGACAGAAA AACAGTACAT TGCAACAATT CACTCTCGTC TTCAGGACTC ACAGCTTTGG 
AAACTGCCTG GGCTCCAAGC CACTGTTAGA CTTGCCTGGG CGCTGGCATT GAGGGGAATA 
TCCCAGCTAC CTGATGTGAC AGCTCTGGCA GAATTCACAG AGGCAGATGA AGCAATGGCA 
GAACTCGCAA TTGCTGACAA CGTTTTCCTG TTCCTCATGG AATCTGTAGT GGTATCAGAA 
TACTTTTATC AGGAAGAATT TTATATTCGC AGAGTCCATA ATCTCATCAC AGATTTCCTT 
GCACTTATGC CAATGAAGGT GAAACAGCTG AGGAATCGGG CAGATGAAGA TGCTCGAATG 
ATTCACATGA GTATGCAGAT GGGTAATGAA CCCCCCATTT CACTTAGAAG GGACCTGGAA 
CACTTAATGC TTTTGATTGG CGAGCTATAT AAAAAGAACC CTTTTCATCT GGAGCTTGCT 
CTAGAATATT GGTGTCCCAC AGAGCCTCTT CAGACTCCGA CTATCATGGG CTCTTATCTA 
GGGGTGGCTC ATCAGCGGCC CCCTCAACGC CAGGTTGTCT TGTCAAAGTT TGTTAGGCAA 
ATGGGTGACC TGTTGCCTCC AACTATTTAT ATTCCTTATT TGAAGATGCT CCAGGGATTG 
GCCAATGGGC CTCAGTGTGC CCACTACTGT TTCAGCCTGC TCAAAGTCAA TGGTAGTAGT 
CATGTTGAAA ATATTCAGGG AGCAGGTGGC AGTCCTGTTT CCTGGGAACA TTTCTTTCAC 
TCCTTGATGC TTTACCACGA ACACCTTCGG AAGGATCTTC CAAGTGCAGA TAGTGTCCAG 
TACCGTCACC TTCCTTCCCG TGGCATCACC CAGAAGGAGC AAGATGGATT GATTGCTTTT 
TTGCAGCTCA CGTCTACCAT CATTACTTGG AGTGAAAATG CTCGCTTGGC ACTCTGTGAA 
CACCCTCAGT GGACCCCTGT TGTGGTGATT CTGGGACTCC TCCAATGCAG TATTCCCCCT 
GTCCTAAAAG CTGAGCTACT GAAGACACTC GCAGCTTTTG GAAAATCTCC TGAAATTGCT 
GCTTCCCTCT GGCAGTCATT GGAATACACT CAGATACTGC AGACCGTGAG GATTCCAAGC 
CAAAGGCAAG CTATTGGTAT TGAGGTTGAA CTAAATGAAA TAGAATCCCG GTGTGAAGAA 
TACCCATTGA CTCGGGCCTT TTGCCAGCTT ATTAGTACTC TGGTGGAGAG CTCATTTCCT 
TCTAATTTGG GTGCTGGACT GCGGCCCCCT GGCTTTGACC CTTATTTGCA GTTCCTTAGA 
GACTCTGTGT TTCTACGATT CCGTACAAGA GCTTACCGGA GAGCAGCTGA AAAGTGGGAA 
GTTGCTGAGG TGGTTTTGGA GGTGTTTTAT AAATTGCTCA GAGATTATGA GCCTCAGCTT 
GAAGATTTTG TAGACCAGTT TGTGGAACTA CAAGGAGAAG AAATCATAGC CTATAAGCCA 
CCTGGATTTA GTCTGATGTA TCATCTGCTG AATGAGTCAC CAATGTTGGA GCTTGCTCTC 
AGTTTACTGG AAGAAGGAGT TAAGCAGCTT GACACCTATG CCCCCTTTCC TGGGAAAAAA 
CACCTGGAGA AAGCAGTACA GCATTGCCTT GCACTTCTCA ATCTTACTCT GCAAAAGGAA 
AATCTTTTTA TGGACCTTCT AAGAGAGAGT CAACTGGCTC TAATAGTCTG TCCTTTAGAA 
CAGCTTTTGC AGGGAATTAA TCCCAGAACT AAGAAGGCAG ATAATGTGGT AAACATTGCC 
AGATACCTAT ATCATGGCAA TACTAATCCA GAATTGGCTT TTGAAAGTGC CAAGATCCTC 
TGTTGTATCT CTTGCAACTC TAATATTCAG ATAAAGTTGG TTGGAGATTT CACACATGAC 
CAGAGTATAA GTCAGAAACT AATGGCTGGA TTTGTGGAGT GTTTGGATTG TGAAGATGCA 
GAAGAATTTG TACGTCTGGA AGAGGGATCA GAACTTGAAA AGAAATTAGT TGCAATTCGT 
CATGAAACAA GAATCCACAT CTTGAATCTT CTCATTACCT CTCTGGAATG CAATCCACCC 
AATCTTGCTC TCTACCTGTT GGGCTTTGAA TTGAAAAAAC CTGTCAGTAC TACAAACCTA 
CAAGATCCAG GAGTGTTAGG TTGCCCTCGG ACATGCCTTC ACGCCATTCT AAACATCTTG 
GAGAAAGGAA CGGAAGGGAG AACAGGCCCA GTGGCGGTGC GAGAATCTCC TCAGCTGGCT 
GAGCTATGTT ACCAGGTCAT ATATCAGTTA TGTGCATGCT CTGATACATC TGGTCCTACT 
ATGAGGTACT TGAGAACCAG CCAGGATTTC TTATTTTCCC AGTTGCAGTA TCTACCATTT 
TCTAACAAAG AATATGAAAT ATCTATGCTG AACCAGATGT CATGGCTTAT GAAAACTGCC 
TCAATAGAGC TAAGGGTAAC CTCTCTGAAT CGTCAGCGGT CACATACCCA GAGGCTCCTA 
CACCTCTTAC TGGATGACAT GCCAGTGAAA CCATACTCAG ATGGTGAAGG AGGAATAGAA 
GATGAAAACA GGTCTGTTTC TGGGTTCCTT CACTTTGACA CTGCTACAAA AGTACGTCGA 
AAAATTCTAA ATATTCTTGA CTCGATTGAC TTCAGTCAGG AGATCCCTGA GCCTTTGCAG 
TTGGATTTTT TTGATCGGGC CCAGATTGAA CAAGTTATTG CTAACTGTGA ACACAAGAAT 
TTACGGGGAC AGACAGTCTG CAATGTCAAG CTTCTTCATA GGGTTCTTGT AGCTGAAGTA 
AATGCCCTTC AGGGTATGGC AGCCATAGGA CAGAGACCTC TACTAATGGA GGAAATCAGC 
ACTGTACTTC AGTATGTGGT AGGAAGAAAT AAATTGCTGC AGTGTCTTCA TGCAAAACGT 
CATGCTCTGG AGTCGTGGAG GCAACTAGTA GAAATTATAC TGACAGCTTG TCCCCAGGAC 
CTCATTCAGG CAGAGGATCG ACAACTGATT ATTCGTGATA TTTTACAAGA TGTGCATGAT 
AAGATACTGG ATGATGAAGC TGCGCAAGAG TTAATGCCTG TGGTCGCCGG GGCAGTGTTC 
ACACTGACTG CTCACCTAAG CCAGGCCGTC CTCACTGAAC AGAAGGAAAC ATCAGTCTTG 
GGACCAGCAG AGGCCCATTA CGCTTTTATG CTTGATAGTT GCTTCACCTC ACCTCCTCCT 
GAAGAGAACC CATTAGTGGG TTTTGCTTCT ATTGGAGATT CTTCACTTTA CATCATATTG 
AAGAAACTGT TAGACTTCAT TTTGAAGACA GGTGGTGGAT TCCAACGAGT GAGGACTCAC 
TTGTATGGCT CTCTGCTTTA TTACTTACAG ATTGCCCAGA GACCTGATGA ACCAGACACC 
TTAGAAGCAG CCAAGAAAAC CATGTGGGAA AGGCTGACAG CCCCTGAAGA TGTATTTAGC 
AAATTACAGC GAGAAAACAT AGCCATTATT GAAAGTTATG GCGCCGCCCT CATGGAAGTG 
GTCTGTCGAG ATGCTTGTGA TGGTCATGAG ATTGGAAGGA TGCTGGCCCT GGCTCTACTT 
GATAGAATTG TCTCCGTGGA TAAACAGCAG CAGTGGCTTT TGTATCTTTC TAACAGTGGC 
TACTTGAAGG TCCTCGTAGA CAGCTTGGTA GAAGATGACC GTACTTTGCA GAGCTTACTC 
ACCCCACAGC CTCCCCTTTT AAAAGCACTT TATACTTATG AATCTAAAAT GGCATTTCTC 
ACAAGAGTGG CAAAGATACA GCAGGGTGCA TTAGAGCTGC TAAGATCAGG GGTGATTGTG 
AGACTAGCTC AATGCCAAGT CTATGACATG CGCCCAGAAA CGGACCCGCA GAGCATGTTT 
GGCATGAGAG ACCCTCCAAT GTTCATCCCT ACCCCAGTGG ATCGCTACCG CCAGATTCTC 
CTCCCAGCTC TCCAGCTGTG CCAGGTCATC CTCACATCTA GTATGGCCCA GCACTTGCAG 
GCAGCAGGGC AGGTATTGCA GTTTCTTATT TCACATTCTG ATACCATACA AGCAATTCTG 
CGCTGTCAGG ATGTTAGTGC TGGGTCTTTG CAGGAATTGG CTCTGCTGAC AGGAATTATA 
AGTAAAGCAG CACTTCCTGG AATATTAAGT GAACTTGACG TTGATGTAAA TGAAGGGTCT 
CTAATGGAGC TACAGGGACA TATTGGAAGA TTCCAGCGCC AGTGCTTAGG ACTACTAAGT 
CGCTTTGGTG GCTCTGACAG ACTGCGTCAG TTTAAATTTC AAGACGATAA TGTGGAGGGA 
GATAAAGTAA GCAAGAAAGA TGAGATTGAA CTGGCTATGC AGCAGATTTG TGCCAATGTA 
ATGGAATATT GCCAGTCACT CATGTTACAG AGTTCCCCTA CCTTCCAGCA TGCTGTGTGT 
CTCTTCACTC CTAGCCTTTC AGAAACAGTT AATAGAGATG GACCGCGGCA AGATACCCAA 
GCTCCAGTGG TTCCATACTG GCGCCTGCCT GGTTTAGGCA TTATCATCTA CCTGCTGAAA 
CAGAGTGCTA ATGATTTCTT CAGCTATTAT GACAGTCATC GACAGAGTGT CAGCAAGCTA 
CAAAATGTAG AGCAGCTTCC CCCAGATGAG ATAAAAGAGT TGTGTCAGTC TGTGATGCCT 
GCTGGTGTTG ATAAAATCTC CACTGCTCAG AAATATGTTC TAGCAAGACG GCGCTTGGTG 
AAGGTGATCA ACAATCGAGC TAAACTGCTT TCCCTTTGTT CTTTTATCAT AGAGACCTGC 
CTATTTATTC TTTGGCGCCA TCTGGAGTAC TACTTGTTAC ATTGCATGCC CACGGATTCT 
CAAGATTCCT TATTTGCCTC GAGAACCTTG TTTAAAAGCA GAAGACTGCA AGATTCCTTC 
GCCTCAGAAA CCAATCTAGA TTTTAGAAGT GGGCTGGCTA TAGTGAGCCA ACATGATTTA 
GACCAGCTTC AGGCTGATGC AATCAACGCT TTTGGAGAAT CACTACAAAA GAAACTTCTG 
GACATTGAAG GATTATATTC AAAAGTTCGA TCTCGATATA GTTTCATACA GGCTCTTGTC 
AGACGTATCC GTGGCCTCTT GAGGATATCA AGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055950.2
CDS	29..6067
Translation	MATPLAVNSAASLWGPYKDIWHKVGNALWRRQPEAVHLLDKILKKHKPDFISLFKNPPKNVQQHEKVQKASTEGVAIQGQQGTRLLPEQLIKEAFILSDLFDIGELAAVELLLAGEHQQPHFPGLTRGLVAVLLYWDGKRCIANSLKALIQSRRGKTWTLELSPELASMTTRFTDELMEQGLTYKVLTLVSQIDVNNEFEKLQRERGLGSEKHRKEVSDLIKECRQSLAESLFAWACQSPLGKEDTLLLIGHLERVTVEANGSLDAVNLALLMALLYCFDISFIEQSTEERDDMIHQLPLLTEKQYIATIHSRLQDSQLWKLPGLQATVRLAWALALRGISQLPDVTALAEFTEADEAMAELAIADNVFLFLMESVVVSEYFYQEEFYIRRVHNLITDFLALMPMKVKQLRNRADEDARMIHMSMQMGNEPPISLRRDLEHLMLLIGELYKKNPFHLELALEYWCPTEPLQTPTIMGSYLGVAHQRPPQRQVVLSKFVRQMGDLLPPTIYIPYLKMLQGLANGPQCAHYCFSLLKVNGSSHVENIQGAGGSPVSWEHFFHSLMLYHEHLRKDLPSADSVQYRHLPSRGITQKEQDGLIAFLQLTSTIITWSENARLALCEHPQWTPVVVILGLLQCSIPPVLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRIPSQRQAIGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPSNLGAGLRPPGFDPYLQFLRDSVFLRFRTRAYRRAAEKWEVAEVVLEVFYKLLRDYEPQLEDFVDQFVELQGEEIIAYKPPGFSLMYHLLNESPMLELALSLLEEGVKQLDTYAPFPGKKHLEKAVQHCLALLNLTLQKENLFMDLLRESQLALIVCPLEQLLQGINPRTKKADNVVNIARYLYHGNTNPELAFESAKILCCISCNSNIQIKLVGDFTHDQSISQKLMAGFVECLDCEDAEEFVRLEEGSELEKKLVAIRHETRIHILNLLITSLECNPPNLALYLLGFELKKPVSTTNLQDPGVLGCPRTCLHAILNILEKGTEGRTGPVAVRESPQLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFSQLQYLPFSNKEYEISMLNQMSWLMKTASIELRVTSLNRQRSHTQRLLHLLLDDMPVKPYSDGEGGIEDENRSVSGFLHFDTATKVRRKILNILDSIDFSQEIPEPLQLDFFDRAQIEQVIANCEHKNLRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEISTVLQYVVGRNKLLQCLHAKRHALESWRQLVEIILTACPQDLIQAEDRQLIIRDILQDVHDKILDDEAAQELMPVVAGAVFTLTAHLSQAVLTEQKETSVLGPAEAHYAFMLDSCFTSPPPEENPLVGFASIGDSSLYIILKKLLDFILKTGGGFQRVRTHLYGSLLYYLQIAQRPDEPDTLEAAKKTMWERLTAPEDVFSKLQRENIAIIESYGAALMEVVCRDACDGHEIGRMLALALLDRIVSVDKQQQWLLYLSNSGYLKVLVDSLVEDDRTLQSLLTPQPPLLKALYTYESKMAFLTRVAKIQQGALELLRSGVIVRLAQCQVYDMRPETDPQSMFGMRDPPMFIPTPVDRYRQILLPALQLCQVILTSSMAQHLQAAGQVLQFLISHSDTIQAILRCQDVSAGSLQELALLTGIISKAALPGILSELDVDVNEGSLMELQGHIGRFQRQCLGLLSRFGGSDRLRQFKFQDDNVEGDKVSKKDEIELAMQQICANVMEYCQSLMLQSSPTFQHAVCLFTPSLSETVNRDGPRQDTQAPVVPYWRLPGLGIIIYLLKQSANDFFSYYDSHRQSVSKLQNVEQLPPDEIKELCQSVMPAGVDKISTAQKYVLARRRLVKVINNRAKLLSLCSFIIETCLFILWRHLEYYLLHCMPTDSQDSLFASRTLFKSRRLQDSFASETNLDFRSGLAIVSQHDLDQLQADAINAFGESLQKKLLDIEGLYSKVRSRYSFIQALVRRIRGLLRISRN

Target ORF information:

RefSeq Version	NM_015135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015135.3

ORF Insert Sequence:

ATGGCGACGC CTTTGGCGGT AAATTCGGCT GCTAGTCTAT GGGGTCCTTA CAAAGACATT 
TGGCATAAAG TGGGAAATGC TCTTTGGAGA AGACAACCTG AAGCTGTTCA CCTTCTTGAT 
AAGATTTTGA AGAAACACAA ACCTGACTTC ATCTCATTGT TCAAAAATCC GCCAAAAAAT 
GTTCAACAGC ATGAGAAGGT TCAGAAAGCC AGTACAGAGG GAGTCGCCAT TCAGGGTCAA 
CAGGGAACTC GACTTCTTCC TGAACAGCTC ATTAAAGAAG CCTTTATTCT CAGTGACCTT 
TTTGATATTG GAGAATTGGC AGCTGTTGAG CTTCTTCTTG CTGGAGAGCA TCAACAGCCA 
CATTTTCCTG GCCTTACCAG AGGATTAGTA GCTGTTCTTC TGTACTGGGA TGGAAAGCGA 
TGCATTGCGA ATTCCTTGAA AGCCTTGATA CAGTCTAGAC GGGGAAAGAC ATGGACCCTA 
GAACTCAGTC CAGAGCTGGC TTCCATGACA ACACGCTTTA CAGATGAGCT GATGGAGCAA 
GGATTGACTT ATAAAGTTCT TACGCTGGTG TCACAGATTG ATGTGAATAA TGAGTTTGAG 
AAACTACAGC GAGAGAGAGG TTTGGGCAGT GAAAAACATC GCAAAGAGGT TTCTGATCTC 
ATTAAGGAGT GCAGGCAGTC TCTTGCAGAA AGCCTTTTTG CCTGGGCTTG CCAGTCACCT 
TTAGGCAAAG AAGACACTCT CCTCCTCATT GGACATTTGG AAAGAGTGAC AGTTGAGGCT 
AATGGCTCAC TGGATGCAGT GAATCTGGCT CTTCTTATGG CGCTTCTATA CTGTTTTGAT 
ATCAGTTTTA TAGAGCAAAG CACAGAGGAA CGAGATGATA TGATTCATCA ACTTCCACTG 
TTGACAGAAA AACAGTACAT TGCAACAATT CACTCTCGTC TTCAGGACTC ACAGCTTTGG 
AAACTGCCTG GGCTCCAAGC CACTGTTAGA CTTGCCTGGG CGCTGGCATT GAGGGGAATA 
TCCCAGCTAC CTGATGTGAC AGCTCTGGCA GAATTCACAG AGGCAGATGA AGCAATGGCA 
GAACTCGCAA TTGCTGACAA CGTTTTCCTG TTCCTCATGG AATCTGTAGT GGTATCAGAA 
TACTTTTATC AGGAAGAATT TTATATTCGC AGAGTCCATA ATCTCATCAC AGATTTCCTT 
GCACTTATGC CAATGAAGGT GAAACAGCTG AGGAATCGGG CAGATGAAGA TGCTCGAATG 
ATTCACATGA GTATGCAGAT GGGTAATGAA CCCCCCATTT CACTTAGAAG GGACCTGGAA 
CACTTAATGC TTTTGATTGG CGAGCTATAT AAAAAGAACC CTTTTCATCT GGAGCTTGCT 
CTAGAATATT GGTGTCCCAC AGAGCCTCTT CAGACTCCGA CTATCATGGG CTCTTATCTA 
GGGGTGGCTC ATCAGCGGCC CCCTCAACGC CAGGTTGTCT TGTCAAAGTT TGTTAGGCAA 
ATGGGTGACC TGTTGCCTCC AACTATTTAT ATTCCTTATT TGAAGATGCT CCAGGGATTG 
GCCAATGGGC CTCAGTGTGC CCACTACTGT TTCAGCCTGC TCAAAGTCAA TGGTAGTAGT 
CATGTTGAAA ATATTCAGGG AGCAGGTGGC AGTCCTGTTT CCTGGGAACA TTTCTTTCAC 
TCCTTGATGC TTTACCACGA ACACCTTCGG AAGGATCTTC CAAGTGCAGA TAGTGTCCAG 
TACCGTCACC TTCCTTCCCG TGGCATCACC CAGAAGGAGC AAGATGGATT GATTGCTTTT 
TTGCAGCTCA CGTCTACCAT CATTACTTGG AGTGAAAATG CTCGCTTGGC ACTCTGTGAA 
CACCCTCAGT GGACCCCTGT TGTGGTGATT CTGGGACTCC TCCAATGCAG TATTCCCCCT 
GTCCTAAAAG CTGAGCTACT GAAGACACTC GCAGCTTTTG GAAAATCTCC TGAAATTGCT 
GCTTCCCTCT GGCAGTCATT GGAATACACT CAGATACTGC AGACCGTGAG GATTCCAAGC 
CAAAGGCAAG CTATTGGTAT TGAGGTTGAA CTAAATGAAA TAGAATCCCG GTGTGAAGAA 
TACCCATTGA CTCGGGCCTT TTGCCAGCTT ATTAGTACTC TGGTGGAGAG CTCATTTCCT 
TCTAATTTGG GTGCTGGACT GCGGCCCCCT GGCTTTGACC CTTATTTGCA GTTCCTTAGA 
GACTCTGTGT TTCTACGATT CCGTACAAGA GCTTACCGGA GAGCAGCTGA AAAGTGGGAA 
GTTGCTGAGG TGGTTTTGGA GGTGTTTTAT AAATTGCTCA GAGATTATGA GCCTCAGCTT 
GAAGATTTTG TAGACCAGTT TGTGGAACTA CAAGGAGAAG AAATCATAGC CTATAAGCCA 
CCTGGATTTA GTCTGATGTA TCATCTGCTG AATGAGTCAC CAATGTTGGA GCTTGCTCTC 
AGTTTACTGG AAGAAGGAGT TAAGCAGCTT GACACCTATG CCCCCTTTCC TGGGAAAAAA 
CACCTGGAGA AAGCAGTACA GCATTGCCTT GCACTTCTCA ATCTTACTCT GCAAAAGGAA 
AATCTTTTTA TGGACCTTCT AAGAGAGAGT CAACTGGCTC TAATAGTCTG TCCTTTAGAA 
CAGCTTTTGC AGGGAATTAA TCCCAGAACT AAGAAGGCAG ATAATGTGGT AAACATTGCC 
AGATACCTAT ATCATGGCAA TACTAATCCA GAATTGGCTT TTGAAAGTGC CAAGATCCTC 
TGTTGTATCT CTTGCAACTC TAATATTCAG ATAAAGTTGG TTGGAGATTT CACACATGAC 
CAGAGTATAA GTCAGAAACT AATGGCTGGA TTTGTGGAGT GTTTGGATTG TGAAGATGCA 
GAAGAATTTG TACGTCTGGA AGAGGGATCA GAACTTGAAA AGAAATTAGT TGCAATTCGT 
CATGAAACAA GAATCCACAT CTTGAATCTT CTCATTACCT CTCTGGAATG CAATCCACCC 
AATCTTGCTC TCTACCTGTT GGGCTTTGAA TTGAAAAAAC CTGTCAGTAC TACAAACCTA 
CAAGATCCAG GAGTGTTAGG TTGCCCTCGG ACATGCCTTC ACGCCATTCT AAACATCTTG 
GAGAAAGGAA CGGAAGGGAG AACAGGCCCA GTGGCGGTGC GAGAATCTCC TCAGCTGGCT 
GAGCTATGTT ACCAGGTCAT ATATCAGTTA TGTGCATGCT CTGATACATC TGGTCCTACT 
ATGAGGTACT TGAGAACCAG CCAGGATTTC TTATTTTCCC AGTTGCAGTA TCTACCATTT 
TCTAACAAAG AATATGAAAT ATCTATGCTG AACCAGATGT CATGGCTTAT GAAAACTGCC 
TCAATAGAGC TAAGGGTAAC CTCTCTGAAT CGTCAGCGGT CACATACCCA GAGGCTCCTA 
CACCTCTTAC TGGATGACAT GCCAGTGAAA CCATACTCAG ATGGTGAAGG AGGAATAGAA 
GATGAAAACA GGTCTGTTTC TGGGTTCCTT CACTTTGACA CTGCTACAAA AGTACGTCGA 
AAAATTCTAA ATATTCTTGA CTCGATTGAC TTCAGTCAGG AGATCCCTGA GCCTTTGCAG 
TTGGATTTTT TTGATCGGGC CCAGATTGAA CAAGTTATTG CTAACTGTGA ACACAAGAAT 
TTACGGGGAC AGACAGTCTG CAATGTCAAG CTTCTTCATA GGGTTCTTGT AGCTGAAGTA 
AATGCCCTTC AGGGTATGGC AGCCATAGGA CAGAGACCTC TACTAATGGA GGAAATCAGC 
ACTGTACTTC AGTATGTGGT AGGAAGAAAT AAATTGCTGC AGTGTCTTCA TGCAAAACGT 
CATGCTCTGG AGTCGTGGAG GCAACTAGTA GAAATTATAC TGACAGCTTG TCCCCAGGAC 
CTCATTCAGG CAGAGGATCG ACAACTGATT ATTCGTGATA TTTTACAAGA TGTGCATGAT 
AAGATACTGG ATGATGAAGC TGCGCAAGAG TTAATGCCTG TGGTCGCCGG GGCAGTGTTC 
ACACTGACTG CTCACCTAAG CCAGGCCGTC CTCACTGAAC AGAAGGAAAC ATCAGTCTTG 
GGACCAGCAG AGGCCCATTA CGCTTTTATG CTTGATAGTT GCTTCACCTC ACCTCCTCCT 
GAAGAGAACC CATTAGTGGG TTTTGCTTCT ATTGGAGATT CTTCACTTTA CATCATATTG 
AAGAAACTGT TAGACTTCAT TTTGAAGACA GGTGGTGGAT TCCAACGAGT GAGGACTCAC 
TTGTATGGCT CTCTGCTTTA TTACTTACAG ATTGCCCAGA GACCTGATGA ACCAGACACC 
TTAGAAGCAG CCAAGAAAAC CATGTGGGAA AGGCTGACAG CCCCTGAAGA TGTATTTAGC 
AAATTACAGC GAGAAAACAT AGCCATTATT GAAAGTTATG GCGCCGCCCT CATGGAAGTG 
GTCTGTCGAG ATGCTTGTGA TGGTCATGAG ATTGGAAGGA TGCTGGCCCT GGCTCTACTT 
GATAGAATTG TCTCCGTGGA TAAACAGCAG CAGTGGCTTT TGTATCTTTC TAACAGTGGC 
TACTTGAAGG TCCTCGTAGA CAGCTTGGTA GAAGATGACC GTACTTTGCA GAGCTTACTC 
ACCCCACAGC CTCCCCTTTT AAAAGCACTT TATACTTATG AATCTAAAAT GGCATTTCTC 
ACAAGAGTGG CAAAGATACA GCAGGGTGCA TTAGAGCTGC TAAGATCAGG GGTGATTGTG 
AGACTAGCTC AATGCCAAGT CTATGACATG CGCCCAGAAA CGGACCCGCA GAGCATGTTT 
GGCATGAGAG ACCCTCCAAT GTTCATCCCT ACCCCAGTGG ATCGCTACCG CCAGATTCTC 
CTCCCAGCTC TCCAGCTGTG CCAGGTCATC CTCACATCTA GTATGGCCCA GCACTTGCAG 
GCAGCAGGGC AGGTATTGCA GTTTCTTATT TCACATTCTG ATACCATACA AGCAATTCTG 
CGCTGTCAGG ATGTTAGTGC TGGGTCTTTG CAGGAATTGG CTCTGCTGAC AGGAATTATA 
AGTAAAGCAG CACTTCCTGG AATATTAAGT GAACTTGACG TTGATGTAAA TGAAGGGTCT 
CTAATGGAGC TACAGGGACA TATTGGAAGA TTCCAGCGCC AGTGCTTAGG ACTACTAAGT 
CGCTTTGGTG GCTCTGACAG ACTGCGTCAG TTTAAATTTC AAGACGATAA TGTGGAGGGA 
GATAAAGTAA GCAAGAAAGA TGAGATTGAA CTGGCTATGC AGCAGATTTG TGCCAATGTA 
ATGGAATATT GCCAGTCACT CATGTTACAG AGTTCCCCTA CCTTCCAGCA TGCTGTGTGT 
CTCTTCACTC CTAGCCTTTC AGAAACAGTT AATAGAGATG GACCGCGGCA AGATACCCAA 
GCTCCAGTGG TTCCATACTG GCGCCTGCCT GGTTTAGGCA TTATCATCTA CCTGCTGAAA 
CAGAGTGCTA ATGATTTCTT CAGCTATTAT GACAGTCATC GACAGAGTGT CAGCAAGCTA 
CAAAATGTAG AGCAGCTTCC CCCAGATGAG ATAAAAGAGT TGTGTCAGTC TGTGATGCCT 
GCTGGTGTTG ATAAAATCTC CACTGCTCAG AAATATGTTC TAGCAAGACG GCGCTTGGTG 
AAGGTGATCA ACAATCGAGC TAAACTGCTT TCCCTTTGTT CTTTTATCAT AGAGACCTGC 
CTATTTATTC TTTGGCGCCA TCTGGAGTAC TACTTGTTAC ATTGCATGCC CACGGATTCT 
CAAGATTCCT TATTTGCCTC GAGAACCTTG TTTAAAAGCA GAAGACTGCA AGATTCCTTC 
GCCTCAGAAA CCAATCTAGA TTTTAGAAGT GGGCTGGCTA TAGTGAGCCA ACATGATTTA 
GACCAGCTTC AGGCTGATGC AATCAACGCT TTTGGAGAAT CACTACAAAA GAAACTTCTG 
GACATTGAAG GATTATATTC AAAAGTTCGA TCTCGATATA GTTTCATACA GGCTCTTGTC 
AGACGTATCC GTGGCCTCTT GAGGATATCA AGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP205 ( NM_015135.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055950.1 Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.

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CloneID	OHu12858
Clone ID Related Accession (Same CDS sequence)	NM_015135.2
Accession Version	NM_015135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup205 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA111772.1, BC146784.1 and AC093107.3. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2009 this sequence version replaced NM_015135.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146784.1, SRR1660807.166184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGC CTTTGGCGGT AAATTCGGCT GCTAGTCTAT GGGGTCCTTA CAAAGACATT 
TGGCATAAAG TGGGAAATGC TCTTTGGAGA AGACAACCTG AAGCTGTTCA CCTTCTTGAT 
AAGATTTTGA AGAAACACAA ACCTGACTTC ATCTCATTGT TCAAAAATCC GCCAAAAAAT 
GTTCAACAGC ATGAGAAGGT TCAGAAAGCC AGTACAGAGG GAGTCGCCAT TCAGGGTCAA 
CAGGGAACTC GACTTCTTCC TGAACAGCTC ATTAAAGAAG CCTTTATTCT CAGTGACCTT 
TTTGATATTG GAGAATTGGC AGCTGTTGAG CTTCTTCTTG CTGGAGAGCA TCAACAGCCA 
CATTTTCCTG GCCTTACCAG AGGATTAGTA GCTGTTCTTC TGTACTGGGA TGGAAAGCGA 
TGCATTGCGA ATTCCTTGAA AGCCTTGATA CAGTCTAGAC GGGGAAAGAC ATGGACCCTA 
GAACTCAGTC CAGAGCTGGC TTCCATGACA ACACGCTTTA CAGATGAGCT GATGGAGCAA 
GGATTGACTT ATAAAGTTCT TACGCTGGTG TCACAGATTG ATGTGAATAA TGAGTTTGAG 
AAACTACAGC GAGAGAGAGG TTTGGGCAGT GAAAAACATC GCAAAGAGGT TTCTGATCTC 
ATTAAGGAGT GCAGGCAGTC TCTTGCAGAA AGCCTTTTTG CCTGGGCTTG CCAGTCACCT 
TTAGGCAAAG AAGACACTCT CCTCCTCATT GGACATTTGG AAAGAGTGAC AGTTGAGGCT 
AATGGCTCAC TGGATGCAGT GAATCTGGCT CTTCTTATGG CGCTTCTATA CTGTTTTGAT 
ATCAGTTTTA TAGAGCAAAG CACAGAGGAA CGAGATGATA TGATTCATCA ACTTCCACTG 
TTGACAGAAA AACAGTACAT TGCAACAATT CACTCTCGTC TTCAGGACTC ACAGCTTTGG 
AAACTGCCTG GGCTCCAAGC CACTGTTAGA CTTGCCTGGG CGCTGGCATT GAGGGGAATA 
TCCCAGCTAC CTGATGTGAC AGCTCTGGCA GAATTCACAG AGGCAGATGA AGCAATGGCA 
GAACTCGCAA TTGCTGACAA CGTTTTCCTG TTCCTCATGG AATCTGTAGT GGTATCAGAA 
TACTTTTATC AGGAAGAATT TTATATTCGC AGAGTCCATA ATCTCATCAC AGATTTCCTT 
GCACTTATGC CAATGAAGGT GAAACAGCTG AGGAATCGGG CAGATGAAGA TGCTCGAATG 
ATTCACATGA GTATGCAGAT GGGTAATGAA CCCCCCATTT CACTTAGAAG GGACCTGGAA 
CACTTAATGC TTTTGATTGG CGAGCTATAT AAAAAGAACC CTTTTCATCT GGAGCTTGCT 
CTAGAATATT GGTGTCCCAC AGAGCCTCTT CAGACTCCGA CTATCATGGG CTCTTATCTA 
GGGGTGGCTC ATCAGCGGCC CCCTCAACGC CAGGTTGTCT TGTCAAAGTT TGTTAGGCAA 
ATGGGTGACC TGTTGCCTCC AACTATTTAT ATTCCTTATT TGAAGATGCT CCAGGGATTG 
GCCAATGGGC CTCAGTGTGC CCACTACTGT TTCAGCCTGC TCAAAGTCAA TGGTAGTAGT 
CATGTTGAAA ATATTCAGGG AGCAGGTGGC AGTCCTGTTT CCTGGGAACA TTTCTTTCAC 
TCCTTGATGC TTTACCACGA ACACCTTCGG AAGGATCTTC CAAGTGCAGA TAGTGTCCAG 
TACCGTCACC TTCCTTCCCG TGGCATCACC CAGAAGGAGC AAGATGGATT GATTGCTTTT 
TTGCAGCTCA CGTCTACCAT CATTACTTGG AGTGAAAATG CTCGCTTGGC ACTCTGTGAA 
CACCCTCAGT GGACCCCTGT TGTGGTGATT CTGGGACTCC TCCAATGCAG TATTCCCCCT 
GTCCTAAAAG CTGAGCTACT GAAGACACTC GCAGCTTTTG GAAAATCTCC TGAAATTGCT 
GCTTCCCTCT GGCAGTCATT GGAATACACT CAGATACTGC AGACCGTGAG GATTCCAAGC 
CAAAGGCAAG CTATTGGTAT TGAGGTTGAA CTAAATGAAA TAGAATCCCG GTGTGAAGAA 
TACCCATTGA CTCGGGCCTT TTGCCAGCTT ATTAGTACTC TGGTGGAGAG CTCATTTCCT 
TCTAATTTGG GTGCTGGACT GCGGCCCCCT GGCTTTGACC CTTATTTGCA GTTCCTTAGA 
GACTCTGTGT TTCTACGATT CCGTACAAGA GCTTACCGGA GAGCAGCTGA AAAGTGGGAA 
GTTGCTGAGG TGGTTTTGGA GGTGTTTTAT AAATTGCTCA GAGATTATGA GCCTCAGCTT 
GAAGATTTTG TAGACCAGTT TGTGGAACTA CAAGGAGAAG AAATCATAGC CTATAAGCCA 
CCTGGATTTA GTCTGATGTA TCATCTGCTG AATGAGTCAC CAATGTTGGA GCTTGCTCTC 
AGTTTACTGG AAGAAGGAGT TAAGCAGCTT GACACCTATG CCCCCTTTCC TGGGAAAAAA 
CACCTGGAGA AAGCAGTACA GCATTGCCTT GCACTTCTCA ATCTTACTCT GCAAAAGGAA 
AATCTTTTTA TGGACCTTCT AAGAGAGAGT CAACTGGCTC TAATAGTCTG TCCTTTAGAA 
CAGCTTTTGC AGGGAATTAA TCCCAGAACT AAGAAGGCAG ATAATGTGGT AAACATTGCC 
AGATACCTAT ATCATGGCAA TACTAATCCA GAATTGGCTT TTGAAAGTGC CAAGATCCTC 
TGTTGTATCT CTTGCAACTC TAATATTCAG ATAAAGTTGG TTGGAGATTT CACACATGAC 
CAGAGTATAA GTCAGAAACT AATGGCTGGA TTTGTGGAGT GTTTGGATTG TGAAGATGCA 
GAAGAATTTG TACGTCTGGA AGAGGGATCA GAACTTGAAA AGAAATTAGT TGCAATTCGT 
CATGAAACAA GAATCCACAT CTTGAATCTT CTCATTACCT CTCTGGAATG CAATCCACCC 
AATCTTGCTC TCTACCTGTT GGGCTTTGAA TTGAAAAAAC CTGTCAGTAC TACAAACCTA 
CAAGATCCAG GAGTGTTAGG TTGCCCTCGG ACATGCCTTC ACGCCATTCT AAACATCTTG 
GAGAAAGGAA CGGAAGGGAG AACAGGCCCA GTGGCGGTGC GAGAATCTCC TCAGCTGGCT 
GAGCTATGTT ACCAGGTCAT ATATCAGTTA TGTGCATGCT CTGATACATC TGGTCCTACT 
ATGAGGTACT TGAGAACCAG CCAGGATTTC TTATTTTCCC AGTTGCAGTA TCTACCATTT 
TCTAACAAAG AATATGAAAT ATCTATGCTG AACCAGATGT CATGGCTTAT GAAAACTGCC 
TCAATAGAGC TAAGGGTAAC CTCTCTGAAT CGTCAGCGGT CACATACCCA GAGGCTCCTA 
CACCTCTTAC TGGATGACAT GCCAGTGAAA CCATACTCAG ATGGTGAAGG AGGAATAGAA 
GATGAAAACA GGTCTGTTTC TGGGTTCCTT CACTTTGACA CTGCTACAAA AGTACGTCGA 
AAAATTCTAA ATATTCTTGA CTCGATTGAC TTCAGTCAGG AGATCCCTGA GCCTTTGCAG 
TTGGATTTTT TTGATCGGGC CCAGATTGAA CAAGTTATTG CTAACTGTGA ACACAAGAAT 
TTACGGGGAC AGACAGTCTG CAATGTCAAG CTTCTTCATA GGGTTCTTGT AGCTGAAGTA 
AATGCCCTTC AGGGTATGGC AGCCATAGGA CAGAGACCTC TACTAATGGA GGAAATCAGC 
ACTGTACTTC AGTATGTGGT AGGAAGAAAT AAATTGCTGC AGTGTCTTCA TGCAAAACGT 
CATGCTCTGG AGTCGTGGAG GCAACTAGTA GAAATTATAC TGACAGCTTG TCCCCAGGAC 
CTCATTCAGG CAGAGGATCG ACAACTGATT ATTCGTGATA TTTTACAAGA TGTGCATGAT 
AAGATACTGG ATGATGAAGC TGCGCAAGAG TTAATGCCTG TGGTCGCCGG GGCAGTGTTC 
ACACTGACTG CTCACCTAAG CCAGGCCGTC CTCACTGAAC AGAAGCAAAC ATCAGTCTTG 
GGACCAGCAG AGGCCCATTA CGCTTTTATG CTTGATAGTT GCTTCACCTC ACCTCCTCCT 
GAAGAGAACC CATTAGTGGG TTTTGCTTCT ATTGGAGATT CTTCACTTTA CATCATATTG 
AAGAAACTGT TAGACTTCAT TTTGAAGACA GGTGGTGGAT TCCAACGAGT GAGGACTCAC 
TTGTATGGCT CTCTGCTTTA TTACTTACAG ATTGCCCAGA GACCTGATGA ACCAGACACC 
TTAGAAGCAG CCAAGAAAAC CATGTGGGAA AGGCTGACAG CCCCTGAAGA TGTATTTAGC 
AAATTACAGC GAGAAAACAT AGCCATTATT GAAAGTTATG GCGCCGCCCT CATGGAAGTG 
GTCTGTCGAG ATGCTTGTGA TGGTCATGAG ATTGGAAGGA TGCTGGCCCT GGCTCTACTT 
GATAGAATTG TCTCCGTGGA TAAACAGCAG CAGTGGCTTT TGTATCTTTC TAACAGTGGC 
TACTTGAAGG TCCTCGTAGA CAGCTTGGTA GAAGATGACC GTACTTTGCA GAGCTTACTC 
ACCCCACAGC CTCCCCTTTT AAAAGCACTT TATACTTATG AATCTAAAAT GGCATTTCTC 
ACAAGAGTGG CAAAGATACA GCAGGGTGCA TTAGAGCTGC TAAGATCAGG GGTGATTGTG 
AGACTAGCTC AATGCCAAGT CTATGACATG CGCCCAGAAA CGGACCCGCA GAGCATGTTT 
GGCATGAGAG ACCCTCCAAT GTTCATCCCT ACCCCAGTGG ATCGCTACCG CCAGATTCTC 
CTCCCAGCTC TCCAGCTGTG CCAGGTCATC CTCACATCTA GTATGGCCCA GCACTTGCAG 
GCAGCAGGGC AGGTATTGCA GTTTCTTATT TCACATTCTG ATACCATACA AGCAATTCTG 
CGCTGTCAGG ATGTTAGTGC TGGGTCTTTG CAGGAATTGG CTCTGCTGAC AGGAATTATA 
AGTAAAGCAG CACTTCCTGG AATATTAAGT GAACTTGACG TTGATGTAAA TGAAGGGTCT 
CTAATGGAGC TACAGGGACA TATTGGAAGA TTCCAGCGCC AGTGCTTAGG ACTACTAAGT 
CGCTTTGGTG GCTCTGACAG ACTGCGTCAG TTTAAATTTC AAGACGATAA TGTGGAGGGA 
GATAAAGTAA GCAAGAAAGA TGAGATTGAA CTGGCTATGC AGCAGATTTG TGCCAATGTA 
ATGGAATATT GCCAGTCACT CATGTTACAG AGTTCCCCTA CCTTCCAGCA TGCTGTGTGT 
CTCTTCACTC CTAGCCTTTC AGAAACAGTT AATAGAGATG GACCGCGGCA AGATACCCAA 
GCTCCAGTGG TTCCATACTG GCGCCTGCCT GGTTTAGGCA TTATCATCTA CCTGCTGAAA 
CAGAGTGCTA ATGATTTCTT CAGCTATTAT GACAGTCATC GACAGAGTGT CAGCAAGCTA 
CAAAATGTAG AGCAGCTTCC CCCAGATGAG ATAAAAGAGT TGTGTCAGTC TGTGATGCCT 
GCTGGTGTTG ATAAAATCTC CACTGCTCAG AAATATGTTC TAGCAAGACG GCGCTTGGTG 
AAGGTGATCA ACAATCGAGC TAAACTGCTT TCCCTTTGTT CTTTTATCAT AGAGACCTGC 
CTATTTATTC TTTGGCGCCA TCTGGAGTAC TACTTGTTAC ATTGCATGCC CACGGATTCT 
CAAGATTCCT TATTTGCCTC GAGAACCTTG TTTAAAAGCA GAAGACTGCA AGATTCCTTC 
GCCTCAGAAA CCAATCTAGA TTTTAGAAGT GGGCTGGCTA TAGTGAGCCA ACATGATTTA 
GACCAGCTTC AGGCTGATGC AATCAACGCT TTTGGAGAAT CACTACAAAA GAAACTTCTG 
GACATTGAAG GATTATATTC AAAAGTTCGA TCTCGATATA GTTTCATACA GGCTCTTGTC 
AGACGTATCC GTGGCCTCTT GAGGATATCA AGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055950.1
CDS	32..6070
Translation	MATPLAVNSAASLWGPYKDIWHKVGNALWRRQPEAVHLLDKILKKHKPDFISLFKNPPKNVQQHEKVQKASTEGVAIQGQQGTRLLPEQLIKEAFILSDLFDIGELAAVELLLAGEHQQPHFPGLTRGLVAVLLYWDGKRCIANSLKALIQSRRGKTWTLELSPELASMTTRFTDELMEQGLTYKVLTLVSQIDVNNEFEKLQRERGLGSEKHRKEVSDLIKECRQSLAESLFAWACQSPLGKEDTLLLIGHLERVTVEANGSLDAVNLALLMALLYCFDISFIEQSTEERDDMIHQLPLLTEKQYIATIHSRLQDSQLWKLPGLQATVRLAWALALRGISQLPDVTALAEFTEADEAMAELAIADNVFLFLMESVVVSEYFYQEEFYIRRVHNLITDFLALMPMKVKQLRNRADEDARMIHMSMQMGNEPPISLRRDLEHLMLLIGELYKKNPFHLELALEYWCPTEPLQTPTIMGSYLGVAHQRPPQRQVVLSKFVRQMGDLLPPTIYIPYLKMLQGLANGPQCAHYCFSLLKVNGSSHVENIQGAGGSPVSWEHFFHSLMLYHEHLRKDLPSADSVQYRHLPSRGITQKEQDGLIAFLQLTSTIITWSENARLALCEHPQWTPVVVILGLLQCSIPPVLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRIPSQRQAIGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPSNLGAGLRPPGFDPYLQFLRDSVFLRFRTRAYRRAAEKWEVAEVVLEVFYKLLRDYEPQLEDFVDQFVELQGEEIIAYKPPGFSLMYHLLNESPMLELALSLLEEGVKQLDTYAPFPGKKHLEKAVQHCLALLNLTLQKENLFMDLLRESQLALIVCPLEQLLQGINPRTKKADNVVNIARYLYHGNTNPELAFESAKILCCISCNSNIQIKLVGDFTHDQSISQKLMAGFVECLDCEDAEEFVRLEEGSELEKKLVAIRHETRIHILNLLITSLECNPPNLALYLLGFELKKPVSTTNLQDPGVLGCPRTCLHAILNILEKGTEGRTGPVAVRESPQLAELCYQVIYQLCACSDTSGPTMRYLRTSQDFLFSQLQYLPFSNKEYEISMLNQMSWLMKTASIELRVTSLNRQRSHTQRLLHLLLDDMPVKPYSDGEGGIEDENRSVSGFLHFDTATKVRRKILNILDSIDFSQEIPEPLQLDFFDRAQIEQVIANCEHKNLRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEISTVLQYVVGRNKLLQCLHAKRHALESWRQLVEIILTACPQDLIQAEDRQLIIRDILQDVHDKILDDEAAQELMPVVAGAVFTLTAHLSQAVLTEQKQTSVLGPAEAHYAFMLDSCFTSPPPEENPLVGFASIGDSSLYIILKKLLDFILKTGGGFQRVRTHLYGSLLYYLQIAQRPDEPDTLEAAKKTMWERLTAPEDVFSKLQRENIAIIESYGAALMEVVCRDACDGHEIGRMLALALLDRIVSVDKQQQWLLYLSNSGYLKVLVDSLVEDDRTLQSLLTPQPPLLKALYTYESKMAFLTRVAKIQQGALELLRSGVIVRLAQCQVYDMRPETDPQSMFGMRDPPMFIPTPVDRYRQILLPALQLCQVILTSSMAQHLQAAGQVLQFLISHSDTIQAILRCQDVSAGSLQELALLTGIISKAALPGILSELDVDVNEGSLMELQGHIGRFQRQCLGLLSRFGGSDRLRQFKFQDDNVEGDKVSKKDEIELAMQQICANVMEYCQSLMLQSSPTFQHAVCLFTPSLSETVNRDGPRQDTQAPVVPYWRLPGLGIIIYLLKQSANDFFSYYDSHRQSVSKLQNVEQLPPDEIKELCQSVMPAGVDKISTAQKYVLARRRLVKVINNRAKLLSLCSFIIETCLFILWRHLEYYLLHCMPTDSQDSLFASRTLFKSRRLQDSFASETNLDFRSGLAIVSQHDLDQLQADAINAFGESLQKKLLDIEGLYSKVRSRYSFIQALVRRIRGLLRISRN

Target ORF information:

RefSeq Version	NM_015135.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 205 (NUP205), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015135.2

ORF Insert Sequence:

ATGGCGACGC CTTTGGCGGT AAATTCGGCT GCTAGTCTAT GGGGTCCTTA CAAAGACATT 
TGGCATAAAG TGGGAAATGC TCTTTGGAGA AGACAACCTG AAGCTGTTCA CCTTCTTGAT 
AAGATTTTGA AGAAACACAA ACCTGACTTC ATCTCATTGT TCAAAAATCC GCCAAAAAAT 
GTTCAACAGC ATGAGAAGGT TCAGAAAGCC AGTACAGAGG GAGTCGCCAT TCAGGGTCAA 
CAGGGAACTC GACTTCTTCC TGAACAGCTC ATTAAAGAAG CCTTTATTCT CAGTGACCTT 
TTTGATATTG GAGAATTGGC AGCTGTTGAG CTTCTTCTTG CTGGAGAGCA TCAACAGCCA 
CATTTTCCTG GCCTTACCAG AGGATTAGTA GCTGTTCTTC TGTACTGGGA TGGAAAGCGA 
TGCATTGCGA ATTCCTTGAA AGCCTTGATA CAGTCTAGAC GGGGAAAGAC ATGGACCCTA 
GAACTCAGTC CAGAGCTGGC TTCCATGACA ACACGCTTTA CAGATGAGCT GATGGAGCAA 
GGATTGACTT ATAAAGTTCT TACGCTGGTG TCACAGATTG ATGTGAATAA TGAGTTTGAG 
AAACTACAGC GAGAGAGAGG TTTGGGCAGT GAAAAACATC GCAAAGAGGT TTCTGATCTC 
ATTAAGGAGT GCAGGCAGTC TCTTGCAGAA AGCCTTTTTG CCTGGGCTTG CCAGTCACCT 
TTAGGCAAAG AAGACACTCT CCTCCTCATT GGACATTTGG AAAGAGTGAC AGTTGAGGCT 
AATGGCTCAC TGGATGCAGT GAATCTGGCT CTTCTTATGG CGCTTCTATA CTGTTTTGAT 
ATCAGTTTTA TAGAGCAAAG CACAGAGGAA CGAGATGATA TGATTCATCA ACTTCCACTG 
TTGACAGAAA AACAGTACAT TGCAACAATT CACTCTCGTC TTCAGGACTC ACAGCTTTGG 
AAACTGCCTG GGCTCCAAGC CACTGTTAGA CTTGCCTGGG CGCTGGCATT GAGGGGAATA 
TCCCAGCTAC CTGATGTGAC AGCTCTGGCA GAATTCACAG AGGCAGATGA AGCAATGGCA 
GAACTCGCAA TTGCTGACAA CGTTTTCCTG TTCCTCATGG AATCTGTAGT GGTATCAGAA 
TACTTTTATC AGGAAGAATT TTATATTCGC AGAGTCCATA ATCTCATCAC AGATTTCCTT 
GCACTTATGC CAATGAAGGT GAAACAGCTG AGGAATCGGG CAGATGAAGA TGCTCGAATG 
ATTCACATGA GTATGCAGAT GGGTAATGAA CCCCCCATTT CACTTAGAAG GGACCTGGAA 
CACTTAATGC TTTTGATTGG CGAGCTATAT AAAAAGAACC CTTTTCATCT GGAGCTTGCT 
CTAGAATATT GGTGTCCCAC AGAGCCTCTT CAGACTCCGA CTATCATGGG CTCTTATCTA 
GGGGTGGCTC ATCAGCGGCC CCCTCAACGC CAGGTTGTCT TGTCAAAGTT TGTTAGGCAA 
ATGGGTGACC TGTTGCCTCC AACTATTTAT ATTCCTTATT TGAAGATGCT CCAGGGATTG 
GCCAATGGGC CTCAGTGTGC CCACTACTGT TTCAGCCTGC TCAAAGTCAA TGGTAGTAGT 
CATGTTGAAA ATATTCAGGG AGCAGGTGGC AGTCCTGTTT CCTGGGAACA TTTCTTTCAC 
TCCTTGATGC TTTACCACGA ACACCTTCGG AAGGATCTTC CAAGTGCAGA TAGTGTCCAG 
TACCGTCACC TTCCTTCCCG TGGCATCACC CAGAAGGAGC AAGATGGATT GATTGCTTTT 
TTGCAGCTCA CGTCTACCAT CATTACTTGG AGTGAAAATG CTCGCTTGGC ACTCTGTGAA 
CACCCTCAGT GGACCCCTGT TGTGGTGATT CTGGGACTCC TCCAATGCAG TATTCCCCCT 
GTCCTAAAAG CTGAGCTACT GAAGACACTC GCAGCTTTTG GAAAATCTCC TGAAATTGCT 
GCTTCCCTCT GGCAGTCATT GGAATACACT CAGATACTGC AGACCGTGAG GATTCCAAGC 
CAAAGGCAAG CTATTGGTAT TGAGGTTGAA CTAAATGAAA TAGAATCCCG GTGTGAAGAA 
TACCCATTGA CTCGGGCCTT TTGCCAGCTT ATTAGTACTC TGGTGGAGAG CTCATTTCCT 
TCTAATTTGG GTGCTGGACT GCGGCCCCCT GGCTTTGACC CTTATTTGCA GTTCCTTAGA 
GACTCTGTGT TTCTACGATT CCGTACAAGA GCTTACCGGA GAGCAGCTGA AAAGTGGGAA 
GTTGCTGAGG TGGTTTTGGA GGTGTTTTAT AAATTGCTCA GAGATTATGA GCCTCAGCTT 
GAAGATTTTG TAGACCAGTT TGTGGAACTA CAAGGAGAAG AAATCATAGC CTATAAGCCA 
CCTGGATTTA GTCTGATGTA TCATCTGCTG AATGAGTCAC CAATGTTGGA GCTTGCTCTC 
AGTTTACTGG AAGAAGGAGT TAAGCAGCTT GACACCTATG CCCCCTTTCC TGGGAAAAAA 
CACCTGGAGA AAGCAGTACA GCATTGCCTT GCACTTCTCA ATCTTACTCT GCAAAAGGAA 
AATCTTTTTA TGGACCTTCT AAGAGAGAGT CAACTGGCTC TAATAGTCTG TCCTTTAGAA 
CAGCTTTTGC AGGGAATTAA TCCCAGAACT AAGAAGGCAG ATAATGTGGT AAACATTGCC 
AGATACCTAT ATCATGGCAA TACTAATCCA GAATTGGCTT TTGAAAGTGC CAAGATCCTC 
TGTTGTATCT CTTGCAACTC TAATATTCAG ATAAAGTTGG TTGGAGATTT CACACATGAC 
CAGAGTATAA GTCAGAAACT AATGGCTGGA TTTGTGGAGT GTTTGGATTG TGAAGATGCA 
GAAGAATTTG TACGTCTGGA AGAGGGATCA GAACTTGAAA AGAAATTAGT TGCAATTCGT 
CATGAAACAA GAATCCACAT CTTGAATCTT CTCATTACCT CTCTGGAATG CAATCCACCC 
AATCTTGCTC TCTACCTGTT GGGCTTTGAA TTGAAAAAAC CTGTCAGTAC TACAAACCTA 
CAAGATCCAG GAGTGTTAGG TTGCCCTCGG ACATGCCTTC ACGCCATTCT AAACATCTTG 
GAGAAAGGAA CGGAAGGGAG AACAGGCCCA GTGGCGGTGC GAGAATCTCC TCAGCTGGCT 
GAGCTATGTT ACCAGGTCAT ATATCAGTTA TGTGCATGCT CTGATACATC TGGTCCTACT 
ATGAGGTACT TGAGAACCAG CCAGGATTTC TTATTTTCCC AGTTGCAGTA TCTACCATTT 
TCTAACAAAG AATATGAAAT ATCTATGCTG AACCAGATGT CATGGCTTAT GAAAACTGCC 
TCAATAGAGC TAAGGGTAAC CTCTCTGAAT CGTCAGCGGT CACATACCCA GAGGCTCCTA 
CACCTCTTAC TGGATGACAT GCCAGTGAAA CCATACTCAG ATGGTGAAGG AGGAATAGAA 
GATGAAAACA GGTCTGTTTC TGGGTTCCTT CACTTTGACA CTGCTACAAA AGTACGTCGA 
AAAATTCTAA ATATTCTTGA CTCGATTGAC TTCAGTCAGG AGATCCCTGA GCCTTTGCAG 
TTGGATTTTT TTGATCGGGC CCAGATTGAA CAAGTTATTG CTAACTGTGA ACACAAGAAT 
TTACGGGGAC AGACAGTCTG CAATGTCAAG CTTCTTCATA GGGTTCTTGT AGCTGAAGTA 
AATGCCCTTC AGGGTATGGC AGCCATAGGA CAGAGACCTC TACTAATGGA GGAAATCAGC 
ACTGTACTTC AGTATGTGGT AGGAAGAAAT AAATTGCTGC AGTGTCTTCA TGCAAAACGT 
CATGCTCTGG AGTCGTGGAG GCAACTAGTA GAAATTATAC TGACAGCTTG TCCCCAGGAC 
CTCATTCAGG CAGAGGATCG ACAACTGATT ATTCGTGATA TTTTACAAGA TGTGCATGAT 
AAGATACTGG ATGATGAAGC TGCGCAAGAG TTAATGCCTG TGGTCGCCGG GGCAGTGTTC 
ACACTGACTG CTCACCTAAG CCAGGCCGTC CTCACTGAAC AGAAGCAAAC ATCAGTCTTG 
GGACCAGCAG AGGCCCATTA CGCTTTTATG CTTGATAGTT GCTTCACCTC ACCTCCTCCT 
GAAGAGAACC CATTAGTGGG TTTTGCTTCT ATTGGAGATT CTTCACTTTA CATCATATTG 
AAGAAACTGT TAGACTTCAT TTTGAAGACA GGTGGTGGAT TCCAACGAGT GAGGACTCAC 
TTGTATGGCT CTCTGCTTTA TTACTTACAG ATTGCCCAGA GACCTGATGA ACCAGACACC 
TTAGAAGCAG CCAAGAAAAC CATGTGGGAA AGGCTGACAG CCCCTGAAGA TGTATTTAGC 
AAATTACAGC GAGAAAACAT AGCCATTATT GAAAGTTATG GCGCCGCCCT CATGGAAGTG 
GTCTGTCGAG ATGCTTGTGA TGGTCATGAG ATTGGAAGGA TGCTGGCCCT GGCTCTACTT 
GATAGAATTG TCTCCGTGGA TAAACAGCAG CAGTGGCTTT TGTATCTTTC TAACAGTGGC 
TACTTGAAGG TCCTCGTAGA CAGCTTGGTA GAAGATGACC GTACTTTGCA GAGCTTACTC 
ACCCCACAGC CTCCCCTTTT AAAAGCACTT TATACTTATG AATCTAAAAT GGCATTTCTC 
ACAAGAGTGG CAAAGATACA GCAGGGTGCA TTAGAGCTGC TAAGATCAGG GGTGATTGTG 
AGACTAGCTC AATGCCAAGT CTATGACATG CGCCCAGAAA CGGACCCGCA GAGCATGTTT 
GGCATGAGAG ACCCTCCAAT GTTCATCCCT ACCCCAGTGG ATCGCTACCG CCAGATTCTC 
CTCCCAGCTC TCCAGCTGTG CCAGGTCATC CTCACATCTA GTATGGCCCA GCACTTGCAG 
GCAGCAGGGC AGGTATTGCA GTTTCTTATT TCACATTCTG ATACCATACA AGCAATTCTG 
CGCTGTCAGG ATGTTAGTGC TGGGTCTTTG CAGGAATTGG CTCTGCTGAC AGGAATTATA 
AGTAAAGCAG CACTTCCTGG AATATTAAGT GAACTTGACG TTGATGTAAA TGAAGGGTCT 
CTAATGGAGC TACAGGGACA TATTGGAAGA TTCCAGCGCC AGTGCTTAGG ACTACTAAGT 
CGCTTTGGTG GCTCTGACAG ACTGCGTCAG TTTAAATTTC AAGACGATAA TGTGGAGGGA 
GATAAAGTAA GCAAGAAAGA TGAGATTGAA CTGGCTATGC AGCAGATTTG TGCCAATGTA 
ATGGAATATT GCCAGTCACT CATGTTACAG AGTTCCCCTA CCTTCCAGCA TGCTGTGTGT 
CTCTTCACTC CTAGCCTTTC AGAAACAGTT AATAGAGATG GACCGCGGCA AGATACCCAA 
GCTCCAGTGG TTCCATACTG GCGCCTGCCT GGTTTAGGCA TTATCATCTA CCTGCTGAAA 
CAGAGTGCTA ATGATTTCTT CAGCTATTAT GACAGTCATC GACAGAGTGT CAGCAAGCTA 
CAAAATGTAG AGCAGCTTCC CCCAGATGAG ATAAAAGAGT TGTGTCAGTC TGTGATGCCT 
GCTGGTGTTG ATAAAATCTC CACTGCTCAG AAATATGTTC TAGCAAGACG GCGCTTGGTG 
AAGGTGATCA ACAATCGAGC TAAACTGCTT TCCCTTTGTT CTTTTATCAT AGAGACCTGC 
CTATTTATTC TTTGGCGCCA TCTGGAGTAC TACTTGTTAC ATTGCATGCC CACGGATTCT 
CAAGATTCCT TATTTGCCTC GAGAACCTTG TTTAAAAGCA GAAGACTGCA AGATTCCTTC 
GCCTCAGAAA CCAATCTAGA TTTTAGAAGT GGGCTGGCTA TAGTGAGCCA ACATGATTTA 
GACCAGCTTC AGGCTGATGC AATCAACGCT TTTGGAGAAT CACTACAAAA GAAACTTCTG 
GACATTGAAG GATTATATTC AAAAGTTCGA TCTCGATATA GTTTCATACA GGCTCTTGTC 
AGACGTATCC GTGGCCTCTT GAGGATATCA AGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.
Nature genetics48(4)457-65(2016 Apr)
Braun DA,Sadowski CE,Kohl S,Lovric S,Astrinidis SA,Pabst WL,Gee HY,Ashraf S,Lawson JA,Shril S,Airik M,Tan W,Schapiro D,Rao J,Choi WI,Hermle T,Kemper MJ,Pohl M,Ozaltin F,Konrad M,Bogdanovic R,B?scher R,Helmchen U,Serdaroglu E,Lifton RP,Antonin W,Hildebrandt F

Interaction of adenovirus type 5 E4orf4 with the nuclear pore subunit Nup205 is required for proper viral gene expression.
Journal of virology88(22)13249-59(2014 Nov)
Lu Y,Kucharski TJ,Gamache I,Blanchette P,Branton PE,Teodoro JG

Seventy-five genetic loci influencing the human red blood cell.
Nature492(7429)369-75(2012 Dec)
van der Harst P,Zhang W,Mateo Leach I,Rendon A,Verweij N,Sehmi J,Paul DS,Elling U,Allayee H,Li X,Radhakrishnan A,Tan ST,Voss K,Weichenberger CX,Albers CA,Al-Hussani A,Asselbergs FW,Ciullo M,Danjou F,Dina C,Esko T,Evans DM,Franke L,G?gele M,Hartiala J,Hersch M,Holm H,Hottenga JJ,Kanoni S,Kleber ME,Lagou V,Langenberg C,Lopez LM,Lyytik?inen LP,Melander O,Murgia F,Nolte IM,O'Reilly PF,Padmanabhan S,Parsa A,Pirastu N,Porcu E,Portas L,Prokopenko I,Ried JS,Shin SY,Tang CS,Teumer A,Traglia M,Ulivi S,Westra HJ,Yang J,Zhao JH,Anni F,Abdellaoui A,Attwood A,Balkau B,Bandinelli S,Bastardot F,Benyamin B,Boehm BO,Cookson WO,Das D,de Bakker PI,de Boer RA,de Geus EJ,de Moor MH,Dimitriou M,Domingues FS,D?ring A,Engstr?m G,Eyjolfsson GI,Ferrucci L,Fischer K,Galanello R,Garner SF,Genser B,Gibson QD,Girotto G,Gudbjartsson DF,Harris SE,Hartikainen AL,Hastie CE,Hedblad B,Illig T,Jolley J,K?h?nen M,Kema IP,Kemp JP,Liang L,Lloyd-Jones H,Loos RJ,Meacham S,Medland SE,Meisinger C,Memari Y,Mihailov E,Miller K,Moffatt MF,Nauck M,Novatchkova M,Nutile T,Olafsson I,Onundarson PT,Parracciani D,Penninx BW,Perseu L,Piga A,Pistis G,Pouta A,Puc U,Raitakari O,Ring SM,Robino A,Ruggiero D,Ruokonen A,Saint-Pierre A,Sala C,Salumets A,Sambrook J,Schepers H,Schmidt CO,Sillj? HH,Sladek R,Smit JH,Starr JM,Stephens J,Sulem P,Tanaka T,Thorsteinsdottir U,Tragante V,van Gilst WH,van Pelt LJ,van Veldhuisen DJ,V?lker U,Whitfield JB,Willemsen G,Winkelmann BR,Wirnsberger G,Algra A,Cucca F,d'Adamo AP,Danesh J,Deary IJ,Dominiczak AF,Elliott P,Fortina P,Froguel P,Gasparini P,Greinacher A,Hazen SL,Jarvelin MR,Khaw KT,Lehtim?ki T,Maerz W,Martin NG,Metspalu A,Mitchell BD,Montgomery GW,Moore C,Navis G,Pirastu M,Pramstaller PP,Ramirez-Solis R,Schadt E,Scott J,Shuldiner AR,Smith GD,Smith JG,Snieder H,Sorice R,Spector TD,Stefansson K,Stumvoll M,Tang WH,Toniolo D,T?njes A,Visscher PM,Vollenweider P,Wareham NJ,Wolffenbuttel BH,Boomsma DI,Beckmann JS,Dedoussis GV,Deloukas P,Ferreira MA,Sanna S,Uda M,Hicks AA,Penninger JM,Gieger C,Kooner JS,Ouwehand WH,Soranzo N,Chambers JC

Genome-wide association identifies three new susceptibility loci for Paget's disease of bone.
Nature genetics43(7)685-9(2011 May)
Albagha OM,Wani SE,Visconti MR,Alonso N,Goodman K,Brandi ML,Cundy T,Chung PY,Dargie R,Devogelaer JP,Falchetti A,Fraser WD,Gennari L,Gianfrancesco F,Hooper MJ,Van Hul W,Isaia G,Nicholson GC,Nuti R,Papapoulos S,Montes Jdel P,Ratajczak T,Rea SL,Rendina D,Gonzalez-Sarmiento R,Di Stefano M,Ward LC,Walsh JP,Ralston SH,

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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NUP205 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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