FLG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FLG
Entrez Gene ID	2312
Full Name	filaggrin
Synonyms	ATOD2
General protein information	Preferred Names filaggrin Names filaggrin epidermal filaggrin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Summary	The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009].
Disorder MIM:	135940
Disorder Html:	Ichthyosis vulgaris, 146700 (3); {Dermatitis, atopic, susceptibility to, 2}, 605803 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002016.2	NP_002007.1	filaggrin
NM_002016.1	NP_002007.1	filaggrin

Pathways from BioSystems

Homologies

	LOC712658	XP_001101725.2
Pan troglodytes (chimpanzee)	FLG	XP_003949644.1
Homo sapiens (human)	FLG	NP_002007.1

Gene Ontology

The following FLG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22241	NM_002016.2 Latest version!	Homo sapiens filaggrin (FLG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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OHu22241	NM_002016.1	Homo sapiens filaggrin (FLG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLG ( NM_002016.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002007.1 Homo sapiens filaggrin (FLG), mRNA.

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CloneID	OHu22241
Clone ID Related Accession (Same CDS sequence)	NM_002016.2 , NM_002016.1
Accession Version	NM_002016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	filaggrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356504.5. On Aug 31, 2019 this sequence version replaced NM_002016.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L01090.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTACTC TCCTGGAAAA CATCTTTGCC ATAATTAATC TTTTCAAGCA ATATTCAAAA 
AAAGATAAAA ACACTGACAC ATTGAGTAAA AAAGAGCTGA AGGAACTTCT GGAAAAGGAA 
TTTCGGCAAA TCCTGAAGAA TCCAGATGAC CCAGATATGG TTGATGTCTT CATGGATCAC 
TTGGATATAG ACCACAACAA GAAAATTGAC TTCACTGAGT TTCTTCTGAT GGTATTCAAG 
TTGGCTCAAG CATATTATGA GTCTACCAGA AAAGAGAATT TACCGATATC AGGACACAAG 
CACAGAAAGC ACAGTCATCA TGATAAACAT GAAGATAATA AACAGGAAGA AAACAAAGAA 
AACAGAAAAA GACCCTCAAG TCTGGAAAGA AGAAACAATA GAAAAGGGAA TAAGGGAAGA 
TCCAAGAGCC CAAGAGAAAC AGGGGGGAAA AGGCATGAAT CTAGTTCTGA AAAAAAAGAA 
AGAAAAGGAT ATTCACCTAC TCATAGAGAA GAAGAATATG GAAAAAACCA TCATAACTCA 
AGTAAAAAAG AGAAAAACAA GACTGAAAAT ACTAGATTAG GAGACAATAG GAAGAGGCTA 
AGTGAAAGAC TTGAAGAGAA AGAAGACAAT GAAGAAGGAG TATATGATTA TGAAAATACA 
GGAAGAATGA CTCAAAAATG GATACAATCA GGCCATATTG CCACATATTA CACAATCCAG 
GATGAAGCCT ATGACACCAC TGATAGTCTA TTAGAAGAAA ACAAAATATA TGAAAGATCA 
AGGTCATCTG ATGGCAAATC ATCATCTCAA GTGAACAGGT CAAGACATGA AAATACAAGC 
CAGGTACCAT TGCAGGAGTC CAGGACAAGA AAGCGTAGGG GATCCAGAGT TAGCCAGGAC 
AGGGACAGTG AGGGACACTC AGAAGACTCT GAGAGGCACT CTGGGTCGGC TTCCAGAAAC 
CATCATGGAT CTGCGTGGGA GCAGTCAAGA GATGGCTCCA GACACCCCAG GTCCCATGAT 
GAAGACAGAG CCAGTCATGG GCACTCTGCA GACAGCTCCA GACAATCAGG CACTCGTCAC 
GCAGAGACTT CCTCTCGTGG ACAGACTGCA TCATCCCATG AACAGGCAAG ATCAAGTCCA 
GGAGAAAGAC ATGGATCCGG CCACCAGCAG TCAGCAGACA GCTCCAGACA CTCAGCCACT 
GGGCGCGGGC AAGCTTCATC TGCAGTCAGC GATCGTGGAC ACCGGGGGTC TAGCGGTAGT 
CAGGCCAGTG ACAGTGAGGG ACATTCAGAA AACTCAGACA CACAATCAGT GTCAGGCCAC 
GGAAAGGCTG GGCTGAGACA GCAGAGCCAC CAAGAGTCCA CACGTGGCCG GTCAGGGGAA 
CGGTCTGGAC GTTCAGGGTC TTCCCTCTAC CAGGTGAGCA CTCATGAACA GCCTGACTCT 
GCCCATGGAC GGACCGGGAC CAGCACTGGA GGAAGACAAG GATCGCACCA CGAGCAGGCA 
CGAGACAGCT CCAGGCATTC AGCGTCCCAA GAGGGTCAGG ACACCATTCG TGGACACCCG 
GGGTCAAGCA GAGGAGGAAG GCAGGGATCC CACCACGAGC AATCGGTAAA TAGGTCTGGA 
CACTCAGGTT CCCATCACAG CCACACCACA TCCCAGGGAA GGTCTGATGC CTCCCATGGG 
CAGTCAGGAT CCAGAAGTGC AAGCAGACAA ACACGAAATG AGGAACAATC AGGAGACGGC 
ACCAGGCACT CAGGGTCACG TCATCATGAA GCTTCCTCTC AGGCTGACAG CTCTAGACAC 
TCACAGGTGG GCCAGGGACA ATCATCGGGG CCCAGGACAA GTAGGAACCA GGGATCCAGT 
GTTAGCCAGG ACAGTGACAG TCAGGGACAC TCAGAAGACT CTGAGAGGTG GTCTGGGTCT 
GCTTCCAGAA ACCATCATGG ATCTGCTCAG GAGCAGTCAA GAGATGGCTC CAGACACCCC 
AGGTCCCATC ACGAAGACAG AGCTGGTCAT GGGCACTCTG CAGACAGCTC CAGAAAATCA 
GGCACTCGTC ACACACAGAA TTCCTCTAGT GGACAGGCTG CGTCATCCCA TGAACAGGCA 
AGATCAAGTG CAGGAGAAAG ACATGGATCC CGCCACCAGC TCCAGTCAGC AGACAGCTCC 
AGACACTCAG GCACTGGGCA CGGACAAGCT TCATCTGCAG TCAGAGACAG TGGACACCGA 
GGGTCCAGTG GTAGTCAGGC CACTGACAGT GAGGGACATT CAGAAGACTC AGACACACAG 
TCAGTGTCAG GCCATGGACA GGCTGGTCAC CATCAGCAGA GCCACCAAGA GTCCGCACGT 
GACCGGTCAG GGGAAAGGTC TCGACGTTCA GGGTCTTTCC TCTACCAGGT GAGCACTCAT 
AAACAGTCTG AGTCCTCCCA TGGATGGACA GGGCCCAGCA CTGGAGTAAG ACAAGGATCC 
CACCATGAGC AGGCACGAGA CAACTCCAGG CACTCAGCAT CCCAAGATGG TCAGGACACC 
ATTCGTGGAC ACCCGGGGTC AAGCAGAAGA GGAAGGCAGG GGTCCCACCA CGAGCAATCG 
GTAGATAGGT CTGGACACTC AGGGTCCCAT CACAGCCACA CCACATCCCA GGGAAGGTCT 
GATGCCTCCC GTGGGCAGTC AGGATCCAGA AGTGCAAGCA GAACAACACG TAATGAGGAA 
CAATCAAGAG ACGGCTCCAG GCACTCAGGG TCACGTCACC ATGAAGCTTC CTCTCATGCC 
GACATCTCTA GACACTCACA GGCAGGCCAG GGACAATCAG AGGGGTCCAG GACAAGCAGG 
CGCCAGGGAT CCAGTGTTAG CCAGGACAGT GACAGTGAGG GACATTCAGA AGACTCTGAG 
AGGTGGTCTG GGTCTGCTTC CAGAAACCAT CGTGGATCTG CTCAGGAGCA GTCAAGACAT 
GGCTCCAGAC ACCCCAGGTC CCATCACGAA GACAGAGCCG GTCACGGGCA CTCTGCAGAC 
AGCTCCAGAC AATCAGGAAC TCCTCACGCA GAGACTTCCT CTGGTGGACA GGCTGCGTCA 
TCCCATGAAC AGGCAAGATC AAGTCCAGGA GAAAGACACG GATCCCGCCA CCAGCAGTCA 
GCAGACAGCT CCAGACACTC AGGCATTCCG CGCAGACAAG CTTCATCTGC AGTCAGAGAC 
AGTGGACACT GGGGGTCCAG TGGTAGTCAG GCCAGTGATA GTGAGGGACA TTCAGAGGAG 
TCAGACACAC AGTCAGTGTC AGGCCATGGA CAGGATGGGC CCCATCAGCA GAGCCACCAA 
GAGTCCGCAC GTGACTGGTC AGGGGGAAGG TCTGGACGTT CAGGGTCTTT CATCTACCAG 
GTGAGCACTC ATGAACAGTC TGAGTCTGCC CATGGGCGGA CCAGGACCAG CACTGGACGA 
AGACAAGGAT CCCACCACGA GCAGGCACGA GACAGCTCCA GGCACTCAGC GTCCCAAGAG 
GGTCAGGACA CCATTCGTGC ACACCCGGGG TCAAGGAGAG GAGGAAGGCA GGGATCCCAC 
CATGAGCAAT CGGTAGATAG ATCTGGACAC TCAGGGTCCC ATCACAGCCA CACCACATCC 
CAGGGAAGGT CTGATGCCTC CCATGGGCAG TCAGGATCCA GAAGTGCAAG CAGACAAACT 
CGTAAGGACA AACAATCAGG AGACGGCTCC AGGCACTCAG GGTCACGTCA CCATGAAGCT 
GCCTCTTGGG CTGACAGCTC TAGACACTCA CAGGTGGGAC AGGAACAATC ATCGGGGTCC 
AGGACAAGCA GGCACCAGGG ATCCAGTGTT AGCCAGGACA GTGACAGTGA GAGACACTCA 
GACGACTCCG AGAGGTTGTC TGGGTCTGCT TCCAGAAACC ATCATGGATC TTCTCGGGAG 
CAGTCAAGAG ATGGCTCCAG ACACCCTGGG TTCCATCAAG AAGACAGAGC CAGTCACGGG 
CACTCTGCAG ACAGCTCCAG ACAATCAGGC ACTCATCACA CAGAGTCTTC CTCTCATGGA 
CAGGCTGTGT CATCCCATGA ACAGGCAAGA TCAAGTCCAG GAGAAAGACA TGGATCCCGC 
CACCAGCAGT CAGCAGACAG CTCCAGACAC TCAGGCATTG GGCACAGACA AGCTTCATCT 
GCAGTCAGAG ACAGTGGACA CCGAGGGTCC AGTGGTAGTC AGGTCACTAA CAGTGAGGGA 
CATTCAGAAG ACTCAGACAC ACAGTCAGTG TCAGCCCACG GACAAGCTGG GCCCCATCAG 
CAGAGCCACA AAGAGTCCGC ACGTGGCCAG TCAGGGGAAA GCTCTGGACG TTCAAGGTCT 
TTCCTCTACC AGGTGAGCTC TCATGAACAG TCTGAGTCCA CACACGGACA GACTGCACCC 
AGCACTGGAG GAAGACAAGG ATCCCGCCAT GAGCAGGCAC GAAACAGCTC TAGGCACTCA 
GCATCCCAAG ACGGTCAGGA CACCATTCGT GGACACCCGG GGTCAAGCAG AGGAGGAAGG 
CAGGGATCCT ACCACGAGCA ATCAGTAGAT AGGTCTGGAC ACTCAGGGTA CCATCACAGC 
CACACCACAC CCCAGGGAAG GTCTGATGCC TCCCATGGGC AGTCAGGACC CAGAAGTGCA 
AGCAGGCAAA CAAGAAATGA GGAACAATCA GGAGACGGCT CCAGGCACTC AGGGTCACGT 
CACCATGAAC CTTCCACTCG GGCCGGCAGC TCTAGACACT CACAGGTGGG CCAGGGAGAA 
TCAGCGGGGT CCAAGACAAG CAGGCGCCAG GGATCCAGTG TTAGTCAGGA CAGGGACAGT 
GAGGGACACT CAGAAGACTC TGAGAGGCGG TCTGAGTCGG CTTCCAGAAA CCATTATGGA 
TCTGCTCGGG AGCAGTCAAG ACATGGCTCC AGGAACCCCA GGTCCCATCA AGAAGATAGA 
GCCAGTCATG GGCACTCTGC AGAGAGCTCC AGACAATCAG GCACTCGTCA TGCAGAGACT 
TCCTCTGGTG GACAGGCTGC ATCATCCCAG GAACAGGCAA GGTCAAGTCC AGGAGAAAGA 
CATGGATCCC GCCACCAGCA GTCAGCAGAC AGCTCCACAG ACTCAGGCAC TGGGCGCAGA 
CAAGATTCAT CTGTAGTCGG AGACAGTGGA AACCGAGGGT CCAGTGGTAG CCAGGCCAGT 
GACAGCGAGG GACACTCAGA AGAGTCAGAC ACACAGTCAG TGTCAGCCCA CGGACAGGCT 
GGGCCCCATC AGCAGAGCCA CCAAGAGTCC ACACGTGGCC AGTCAGGGGA AAGGTCTGGA 
CGTTCAGGGT CTTTCCTCTA CCAGGTGAGC ACTCATGAAC AGTCTGAGTC CGCCCATGGA 
CGCACAGGGC CCAGCACTGG AGGAAGACAA AGATCCCGCC ACGAGCAGGC ACGAGACAGC 
TCCAGGCACT CAGCGTCCCA AGAGGGTCAG GACACCATTC GTGGACACCC AGGGTCAAGC 
AGAGGAGGAA GGCAGGGATC CCACTATGAG CAATCGGTAG ATAGTTCTGG ACACTCAGGG 
TCTCATCACA GCCACACCAC GTCCCAGGAA AGGTCTGATG TCTCCCGTGG GCAGTCAGGA 
TCCAGAAGTG TCAGCAGACA AACACGTAAT GAGAAACAAT CAGGAGACGG CTCCAGGCAC 
TCAGGGTCGC GTCACCATGA AGCTTCCTCT CGGGCCGACA GCTCTAGACA CTCGCAGGTG 
GGCCAGGGAC AATCATCAGG GCCCAGGACA AGCAGGAACC AGGGATCCAG TGTTAGCCAG 
GACAGTGACA GTCAGGGACA CTCAGAAGAC TCTGAGAGGT GGTCTGGGTC TGCTTCCAGA 
AACCATCTTG GATCTGCTTG GGAGCAGTCA AGAGATGGCT CCAGACACCC TGGGTCCCAT 
CACGAAGACA GAGCCGGTCA CGGGCACTCT GCAGACAGCT CCAGACAATC AGGCACTCGT 
CACACAGAGT CTTCCTCTCG TGGACAGGCT GCGTCATCCC ATGAACAGGC AAGATCAAGT 
GCAGGAGAAA GACATGGATC CCACCACCAG CTCCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ATGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTACAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCACAGGGAA AAGCTGGGCC CCATCAGCAG AGCCACAAAG AGTCCGCACG TGGCCAGTCA 
GGGGAAAGCT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCCACCC ATGGACAGTC TGCGCCCAGC ACTGGAGGAA GACAAGGATC CCATTATGAT 
CAGGCACAAG ACAGCTCCAG GCACTCAGCA TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGC CAAGCAGAGG AGGAAGACAG GGGTCCCACC AAGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCTCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CGTGGGCAGT CAGGATCCAG AAGTGCAAGC AGAAAAACAT ATGACAAGGA ACAATCAGGA 
GATGGCTCTA GGCACTCAGG GTCGCATCAT CATGAAGCTT CCTCTTGGGC CGACAGCTCT 
AGACACTCAC TGGTGGGCCA GGGACAATCA TCAGGGCCCA GGACAAGCAG GCCCCGGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GGACACTCAG AAGATTCTGA GAGGCGGTCT 
GGGTCTGCGT CCAGAAACCA TCATGGATCT GCTCAGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCAGGT CCCATCACGA AGACAGAGCC GGTCATGGGC ACTCTGCAGA GAGCTCCAGA 
CAATCAGGCA CTCATCATGC AGAGAATTCC TCTGGTGGAC AGGCTGCATC ATCCCATGAA 
CAGGCAAGAT CAAGTGCAGG AGAGAGACAC GGATCCCACC ACCAGCAGTC AGCAGACAGC 
TCCAGACACT CAGGCATTGG GCACGGACAA GCTTCATCTG CAGTCAGAGA CAGTGGACAC 
CGAGGGTCCA GTGGTAGTCA GGCCAGTGAC AGTGAGGGAC ATTCAGAAGA CTCAGACACA 
CAGTCAGTGT CAGCCCACGG ACAGGCTGGG CCCCATCAGC AGAGCCACCA AGAGTCCACA 
CGTGGCCGGT CAGCAGGAAG GTCTGGACGT TCAGGGTCTT TCCTCTACCA GGTGAGCACT 
CATGAACAGT CTGAGTCCGC CCATGGACGG ACCGGGACCA GCACTGGAGG AAGACAAGGA 
TCCCACCACA AGCAGGCACG AGACAGCTCC AGGCACTCAA CGTCCCAAGA GGGTCAGGAC 
ACCATTCATG GACACCCGGG GTCAAGCAGT GGAGGAAGGC AGGGATCCCA CTACGAGCAA 
TTGGTAGATA GATCTGGACA CTCAGGGTCT CATCACAGCC ACACCACATC CCAGGGAAGG 
TCTGATGCCT CCCATGGGCA CTCAGGATCC AGAAGTGCAA GCAGACAAAC TCGTAACGAT 
GAACAATCAG GAGACGGCTC CAGGCACTCA GGGTCGCGTC ACCATGAAGC TTCCTCTCGG 
GCCGACAGCT CTGGACACTC GCAGGTGGGC CAGGGACAAT CAGAGGGGCC CAGGACAAGC 
AGGAACTGGG GATCCAGTTT TAGCCAGGAC AGTGACAGTC AGGGACACTC AGAAGACTCT 
GAGAGGTGGT CTGGGTCTGC TTCCAGAAAC CATCATGGAT CTGCTCAGGA GCAGCTAAGA 
GATGGCTCCA GACACCCCAG GTCCCATCAA GAAGACAGAG CTGGTCATGG GCACTCTGCA 
GACAGCTCCA GACAATCAGG CACTCGTCAC ACACAGACTT CCTCTGGTGG ACAGGCTGCA 
TCATCCCATG AACAGGCAAG ATCAAGTGCA GGAGAAAGAC ATGGATCCCA CCACCAGCAG 
TCAGCAGACA GCTCCAGACA CTCAGGCATT GGGCACGGAC AAGCTTCATC TGCAGTCAGA 
GACAGTGGAC ACCGAGGGTA CAGTGGTAGT CAGGCCAGTG ACAATGAGGG ACATTCAGAA 
GACTCAGACA CACAGTCAGT GTCAGCCCAC GGACAGGCTG GGTCCCATCA GCAGAGCCAC 
CAAGAGTCCG CACGTGGCCG GTCAGGGGAA ACGTCTGGAC ATTCAGGATC TTTCCTCTAC 
CAGGTGAGCA CTCATGAACA GTCTGAGTCC TCCCATGGAT GGACGGGGCC CAGCACTAGA 
GGAAGACAAG GATCCCGCCA TGAGCAGGCA CAAGACAGCT CCAGGCACTC AGCATCCCAA 
GACGGTCAGG ACACCATTCG TGGACACCCG GGGTCAAGCA GAGGAGGAAG GCAGGGGTAC 
CACCACGAGC ATTCGGTAGA TAGCTCTGGA CACTCAGGGT CCCATCACAG CCACACCACA 
TCCCAGGGAA GGTCTGATGC CTCCCGTGGG CAGTCAGGAT CCAGAAGTGC AAGCAGAACA 
ACACGTAATG AGGAACAATC AGGAGACGGC TCCAGGCACT CAGGGTCGCG TCACCATGAA 
GCTTCCACTC ATGCCGACAT CTCTAGACAC TCACAGGCAG TCCAGGGACA ATCAGAGGGG 
TCCAGGAGAA GCAGGCGCCA GGGATCCAGT GTGAGCCAGG ACAGTGACAG TGAGGGACAT 
TCAGAAGACT CTGAGAGGTG GTCTGGGTCT GCTTCCAGAA ACCATCATGG ATCTGCTCAG 
GAGCAGCTAA GAGATGGCTC CAGACACCCC AGGTCCCATC AAGAAGACAG AGCTGGTCAT 
GGGCACTCTG CAGACAGCTC CAGACAATCA GGCACTCGTC ACACACAGAC TTCCTCTGGT 
GGACAGGCTG CATCATCCCA TGAACAGGCA AGATCAAGTG CAGGAGAAAG ACATGGATCC 
CACCACCAGC AGTCAGCAGA CAGCTCCAGA CACTCAGGCA TTGGGCACGG ACAAGCTTCA 
TCTGCAGTCA GAGACAGTGG ACACCGAGGG TACAGTGGTA GTCAGGCCAG TGACAATGAG 
GGACATTCAG AAGACTCAGA CACACAGTCA GTGTCAGCCC ACGGACAGGC TGGGTCCCAT 
CAGCAGAGCC ACCAAGAGTC CGCACGTGGC CGGTCAGGGG AAACGTCTGG ACATTCAGGA 
TCTTTCCTCT ACCAGGTGAG CACTCATGAA CAGTCTGAGT CCTCCCATGG ATGGACGGGG 
CCCAGCACTA GAGGAAGACA AGGATCCCGC CATGAGCAGG CACAAGACAG CTCCAGGCAC 
TCAGCATCCC AATACGGTCA GGACACCATT CGTGGACACC CGGGGTCAAG CAGAGGAGGA 
AGGCAGGGGT ACCACCACGA GCATTCGGTA GATAGCTCTG GACACTCAGG GTCCCATCAC 
AGCCACACCA CATCCCAGGG AAGGTCTGAT GCCTCCCGTG GGCAGTCAGG ATCCAGAAGT 
GCAAGCAGAA CAACACGTAA TGAGGAACAA TCAGGAGACA GCTCCAGGCA CTCAGTGTCA 
CGTCACCATG AAGCTTCCAC TCATGCCGAC ATCTCTAGAC ACTCACAGGC AGTCCAGGGA 
CAATCAGAGG GGTCCAGGAG AAGCAGGCGC CAGGGATCCA GTGTGAGCCA GGACAGTGAC 
AGTGAGGGAC ATTCAGAAGA CTCTGAGAGG TGGTCTGGGT CTGCTTCCAG AAACCATCGT 
GGATCTGTTC AGGAGCAGTC AAGGCACGGC TCCAGACACC CCAGGTCCCA TCACGAAGAC 
AGAGCCGGTC ACGGGCACTC TGCAGACCGC TCCAGACAAT CAGGCACTCG TCACGCAGAG 
ACTTCCTCTG GTGGACAGGC TGCATCATCC CATGAACAGG CAAGATCAAG TCCAGGAGAG 
AGACACGGAT CCCGCCACCA GCAGTCAGCA GACAGCTCCA GACACTCAGG CATTCCGCGT 
GGACAAGCTT CATCTGCAGT CAGAGACAGT AGACACTGGG GGTCCAGTGG TAGTCAGGCC 
AGTGATAGTG AGGGACATTC AGAAGAGTCA GACACACAGT CAGTGTCAGG CCATGGACAG 
GCTGGGCCCC ATCAGCAGAG CCACCAAGAG TCCGCACGTG ACCGGTCAGG GGGAAGGTCT 
GGACGTTCAG GGTCTTTCCT CTACCAGGTG AGCACTCATG AACAGTCTGA GTCTGCCCAT 
GGGCGGACCA GGACCAGCAC TGGACGAAGA CAAGGATCCC ACCACGAGCA GGCACGAGAC 
AGCTCCAGGC ACTCAGCGTC CCAAGAGGGT CAGGACACCA TTCGTGGACA CCCGGGGTCA 
AGCAGAAGAG GAAGGCAGGG ATCCCACTAC GAGCAATCGG TAGATAGGTC TGGACACTCA 
GGGTCCCATC ACAGCCACAC CACATCCCAG GGAAGGTCTG ATGCCTCCCG TGGGCAGTCA 
GGATCCAGAA GTGCCAGCAG ACAAACTCGT AATGACGAAC AATCAGGAGA TGGCTCCAGG 
CACTCATGGT CGCATCACCA TGAAGCTTCC ACTCAGGCGG ACAGCTCTAG ACACTCACAG 
TCCGGCCAGG GACAATCAGC GGGGCCCAGG ACAAGCAGGA ACCAGGGATC CAGTGTTAGC 
CAGGACAGTG ACAGTCAGGG ACACTCAGAA GACTCTGAGA GGTGGTCTGG GTCTGCTTCC 
AGAAACCATC GTGGATCTGC TCAGGAGCAG TCAAGAGATG GCTCCAGACA CCCCACGTCC 
CATCACGAAG ACAGAGCCGG TCACGGGCAC TCTGCAGAGA GCTCCAGACA ATCAGGCACT 
CATCATGCAG AGAATTCCTC TGGTGGACAG GCTGCATCAT CCCATGAACA GGCAAGATCA 
AGTGCAGGAG AGAGACATGG ATCCCACCAC CAGCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ACGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTCCAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCCCACGGAC AGGCTGGGCC CCATCAGCAG AGCCACCAAG AGTCCACACG TGGCCGGTCA 
GCAGGAAGGT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCTGCCC ATGGACGGGC TGGGCCCAGT ACTGGAGGAA GACAAGGATC CCGCCACGAG 
CAGGCACGAG ACAGCTCCAG GCACTCAGCG TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGT CAAGGAGAGG AGGAAGACAG GGATCCTACC ACGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCCCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CATGGGCAGT CAGGATCCAG AAGTGCAAGC AGAGAAACAC GTAATGAGGA ACAGTCAGGA 
GACGGCTCCA GGCACTCAGG GTCGCGTCAC CATGAAGCTT CCACTCAGGC TGACAGCTCT 
AGACACTCAC AGTCCGGCCA GGGTGAATCA GCGGGGTCCA GGAGAAGCAG GCGCCAGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GCATACCCAG AGGACTCTGA GAGGCGATCT 
GAGTCTGCTT CCAGAAACCA TCATGGATCT TCTCGGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCGGAT CCTCTCACCG CGATACAGCC AGTCATGTAC AGTCTTCACC TGTACAGTCA 
GACTCTAGTA CCGCTAAGGA ACATGGTCAC TTTAGTAGTC TTTCACAAGA TTCTGCGTAT 
CACTCAGGAA TACAGTCACG TGGCAGTCCT CACAGTTCTA GTTCTTATCA TTATCAATCT 
GAGGGCACTG AAAGGCAAAA AGGTCAATCA GGTTTAGTTT GGAGACATGG CAGCTATGGT 
AGTGCAGATT ATGATTATGG TGAATCCGGG TTTAGACACT CTCAGCACGG AAGTGTTAGT 
TACAATTCCA ATCCTGTTGT TTTCAAGGAA AGATCTGATA TCTGTAAAGC AAGTGCGTTT 
GGTAAAGATC ATCCAAGGTA TTATGCAACG TATATTAATA AGGACCCAGG TTTATGTGGC 
CATTCTAGTG ATATATCGAA ACAACTGGGA TTTAGTCAGT CACAGAGATA CTATTACTAT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002007.1
CDS	73..12258
Translation	MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQILKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHRKHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKERKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYENTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRHENTSQVPLQESRTRKRRGSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSRHPRSHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSADSSRHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSHQESTRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRNEEQSGDGTRHSGSRHHEASSQADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSADSSRKSGTRHTQNSSSGQAASSHEQARSSAGERHGSRHQLQSADSSRHSGTGHGQASSAVRDSGHRGSSGSQATDSEGHSEDSDTQSVSGHGQAGHHQQSHQESARDRSGERSRRSGSFLYQVSTHKQSESSHGWTGPSTGVRQGSHHEQARDNSRHSASQDGQDTIRGHPGSSRRGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSRDGSRHSGSRHHEASSHADISRHSQAGQGQSEGSRTSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSAQEQSRHGSRHPRSHHEDRAGHGHSADSSRQSGTPHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRRQASSAVRDSGHWGSSGSQASDSEGHSEESDTQSVSGHGQDGPHQQSHQESARDWSGGRSGRSGSFIYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRAHPGSRRGGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRKDKQSGDGSRHSGSRHHEAASWADSSRHSQVGQEQSSGSRTSRHQGSSVSQDSDSERHSDDSERLSGSASRNHHGSSREQSRDGSRHPGFHQEDRASHGHSADSSRQSGTHHTESSSHGQAVSSHEQARSSPGERHGSRHQQSADSSRHSGIGHRQASSAVRDSGHRGSSGSQVTNSEGHSEDSDTQSVSAHGQAGPHQQSHKESARGQSGESSGRSRSFLYQVSSHEQSESTHGQTAPSTGGRQGSRHEQARNSSRHSASQDGQDTIRGHPGSSRGGRQGSYHEQSVDRSGHSGYHHSHTTPQGRSDASHGQSGPRSASRQTRNEEQSGDGSRHSGSRHHEPSTRAGSSRHSQVGQGESAGSKTSRRQGSSVSQDRDSEGHSEDSERRSESASRNHYGSAREQSRHGSRNPRSHQEDRASHGHSAESSRQSGTRHAETSSGGQAASSQEQARSSPGERHGSRHQQSADSSTDSGTGRRQDSSVVGDSGNRGSSGSQASDSEGHSEESDTQSVSAHGQAGPHQQSHQESTRGQSGERSGRSGSFLYQVSTHEQSESAHGRTGPSTGGRQRSRHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHYEQSVDSSGHSGSHHSHTTSQERSDVSRGQSGSRSVSRQTRNEKQSGDGSRHSGSRHHEASSRADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHLGSAWEQSRDGSRHPGSHHEDRAGHGHSADSSRQSGTRHTESSSRGQAASSHEQARSSAGERHGSHHQLQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDSEGHSEDSDTQSVSAQGKAGPHQQSHKESARGQSGESSGRSGSFLYQVSTHEQSESTHGQSAPSTGGRQGSHYDQAQDSSRHSASQEGQDTIRGHPGPSRGGRQGSHQEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRKTYDKEQSGDGSRHSGSHHHEASSWADSSRHSLVGQGQSSGPRTSRPRGSSVSQDSDSEGHSEDSERRSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRTGTSTGGRQGSHHKQARDSSRHSTSQEGQDTIHGHPGSSSGGRQGSHYEQLVDRSGHSGSHHSHTTSQGRSDASHGHSGSRSASRQTRNDEQSGDGSRHSGSRHHEASSRADSSGHSQVGQGQSEGPRTSRNWGSSFSQDSDSQGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQDGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDGSRHSGSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQYGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDSSRHSVSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSVQEQSRHGSRHPRSHHEDRAGHGHSADRSRQSGTRHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRGQASSAVRDSRHWGSSGSQASDSEGHSEESDTQSVSGHGQAGPHQQSHQESARDRSGGRSGRSGSFLYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRRGRQGSHYEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRQTRNDEQSGDGSRHSWSHHHEASTQADSSRHSQSGQGQSAGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHRGSAQEQSRDGSRHPTSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRAGPSTGGRQGSRHEQARDSSRHSASQEGQDTIRGHPGSRRGGRQGSYHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRETRNEEQSGDGSRHSGSRHHEASTQADSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE

Target ORF information:

RefSeq Version	NM_002016.2
Organism	Homo sapiens(human)
Definition	Homo sapiens filaggrin (FLG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002016.2

ORF Insert Sequence:

ATGTCTACTC TCCTGGAAAA CATCTTTGCC ATAATTAATC TTTTCAAGCA ATATTCAAAA 
AAAGATAAAA ACACTGACAC ATTGAGTAAA AAAGAGCTGA AGGAACTTCT GGAAAAGGAA 
TTTCGGCAAA TCCTGAAGAA TCCAGATGAC CCAGATATGG TTGATGTCTT CATGGATCAC 
TTGGATATAG ACCACAACAA GAAAATTGAC TTCACTGAGT TTCTTCTGAT GGTATTCAAG 
TTGGCTCAAG CATATTATGA GTCTACCAGA AAAGAGAATT TACCGATATC AGGACACAAG 
CACAGAAAGC ACAGTCATCA TGATAAACAT GAAGATAATA AACAGGAAGA AAACAAAGAA 
AACAGAAAAA GACCCTCAAG TCTGGAAAGA AGAAACAATA GAAAAGGGAA TAAGGGAAGA 
TCCAAGAGCC CAAGAGAAAC AGGGGGGAAA AGGCATGAAT CTAGTTCTGA AAAAAAAGAA 
AGAAAAGGAT ATTCACCTAC TCATAGAGAA GAAGAATATG GAAAAAACCA TCATAACTCA 
AGTAAAAAAG AGAAAAACAA GACTGAAAAT ACTAGATTAG GAGACAATAG GAAGAGGCTA 
AGTGAAAGAC TTGAAGAGAA AGAAGACAAT GAAGAAGGAG TATATGATTA TGAAAATACA 
GGAAGAATGA CTCAAAAATG GATACAATCA GGCCATATTG CCACATATTA CACAATCCAG 
GATGAAGCCT ATGACACCAC TGATAGTCTA TTAGAAGAAA ACAAAATATA TGAAAGATCA 
AGGTCATCTG ATGGCAAATC ATCATCTCAA GTGAACAGGT CAAGACATGA AAATACAAGC 
CAGGTACCAT TGCAGGAGTC CAGGACAAGA AAGCGTAGGG GATCCAGAGT TAGCCAGGAC 
AGGGACAGTG AGGGACACTC AGAAGACTCT GAGAGGCACT CTGGGTCGGC TTCCAGAAAC 
CATCATGGAT CTGCGTGGGA GCAGTCAAGA GATGGCTCCA GACACCCCAG GTCCCATGAT 
GAAGACAGAG CCAGTCATGG GCACTCTGCA GACAGCTCCA GACAATCAGG CACTCGTCAC 
GCAGAGACTT CCTCTCGTGG ACAGACTGCA TCATCCCATG AACAGGCAAG ATCAAGTCCA 
GGAGAAAGAC ATGGATCCGG CCACCAGCAG TCAGCAGACA GCTCCAGACA CTCAGCCACT 
GGGCGCGGGC AAGCTTCATC TGCAGTCAGC GATCGTGGAC ACCGGGGGTC TAGCGGTAGT 
CAGGCCAGTG ACAGTGAGGG ACATTCAGAA AACTCAGACA CACAATCAGT GTCAGGCCAC 
GGAAAGGCTG GGCTGAGACA GCAGAGCCAC CAAGAGTCCA CACGTGGCCG GTCAGGGGAA 
CGGTCTGGAC GTTCAGGGTC TTCCCTCTAC CAGGTGAGCA CTCATGAACA GCCTGACTCT 
GCCCATGGAC GGACCGGGAC CAGCACTGGA GGAAGACAAG GATCGCACCA CGAGCAGGCA 
CGAGACAGCT CCAGGCATTC AGCGTCCCAA GAGGGTCAGG ACACCATTCG TGGACACCCG 
GGGTCAAGCA GAGGAGGAAG GCAGGGATCC CACCACGAGC AATCGGTAAA TAGGTCTGGA 
CACTCAGGTT CCCATCACAG CCACACCACA TCCCAGGGAA GGTCTGATGC CTCCCATGGG 
CAGTCAGGAT CCAGAAGTGC AAGCAGACAA ACACGAAATG AGGAACAATC AGGAGACGGC 
ACCAGGCACT CAGGGTCACG TCATCATGAA GCTTCCTCTC AGGCTGACAG CTCTAGACAC 
TCACAGGTGG GCCAGGGACA ATCATCGGGG CCCAGGACAA GTAGGAACCA GGGATCCAGT 
GTTAGCCAGG ACAGTGACAG TCAGGGACAC TCAGAAGACT CTGAGAGGTG GTCTGGGTCT 
GCTTCCAGAA ACCATCATGG ATCTGCTCAG GAGCAGTCAA GAGATGGCTC CAGACACCCC 
AGGTCCCATC ACGAAGACAG AGCTGGTCAT GGGCACTCTG CAGACAGCTC CAGAAAATCA 
GGCACTCGTC ACACACAGAA TTCCTCTAGT GGACAGGCTG CGTCATCCCA TGAACAGGCA 
AGATCAAGTG CAGGAGAAAG ACATGGATCC CGCCACCAGC TCCAGTCAGC AGACAGCTCC 
AGACACTCAG GCACTGGGCA CGGACAAGCT TCATCTGCAG TCAGAGACAG TGGACACCGA 
GGGTCCAGTG GTAGTCAGGC CACTGACAGT GAGGGACATT CAGAAGACTC AGACACACAG 
TCAGTGTCAG GCCATGGACA GGCTGGTCAC CATCAGCAGA GCCACCAAGA GTCCGCACGT 
GACCGGTCAG GGGAAAGGTC TCGACGTTCA GGGTCTTTCC TCTACCAGGT GAGCACTCAT 
AAACAGTCTG AGTCCTCCCA TGGATGGACA GGGCCCAGCA CTGGAGTAAG ACAAGGATCC 
CACCATGAGC AGGCACGAGA CAACTCCAGG CACTCAGCAT CCCAAGATGG TCAGGACACC 
ATTCGTGGAC ACCCGGGGTC AAGCAGAAGA GGAAGGCAGG GGTCCCACCA CGAGCAATCG 
GTAGATAGGT CTGGACACTC AGGGTCCCAT CACAGCCACA CCACATCCCA GGGAAGGTCT 
GATGCCTCCC GTGGGCAGTC AGGATCCAGA AGTGCAAGCA GAACAACACG TAATGAGGAA 
CAATCAAGAG ACGGCTCCAG GCACTCAGGG TCACGTCACC ATGAAGCTTC CTCTCATGCC 
GACATCTCTA GACACTCACA GGCAGGCCAG GGACAATCAG AGGGGTCCAG GACAAGCAGG 
CGCCAGGGAT CCAGTGTTAG CCAGGACAGT GACAGTGAGG GACATTCAGA AGACTCTGAG 
AGGTGGTCTG GGTCTGCTTC CAGAAACCAT CGTGGATCTG CTCAGGAGCA GTCAAGACAT 
GGCTCCAGAC ACCCCAGGTC CCATCACGAA GACAGAGCCG GTCACGGGCA CTCTGCAGAC 
AGCTCCAGAC AATCAGGAAC TCCTCACGCA GAGACTTCCT CTGGTGGACA GGCTGCGTCA 
TCCCATGAAC AGGCAAGATC AAGTCCAGGA GAAAGACACG GATCCCGCCA CCAGCAGTCA 
GCAGACAGCT CCAGACACTC AGGCATTCCG CGCAGACAAG CTTCATCTGC AGTCAGAGAC 
AGTGGACACT GGGGGTCCAG TGGTAGTCAG GCCAGTGATA GTGAGGGACA TTCAGAGGAG 
TCAGACACAC AGTCAGTGTC AGGCCATGGA CAGGATGGGC CCCATCAGCA GAGCCACCAA 
GAGTCCGCAC GTGACTGGTC AGGGGGAAGG TCTGGACGTT CAGGGTCTTT CATCTACCAG 
GTGAGCACTC ATGAACAGTC TGAGTCTGCC CATGGGCGGA CCAGGACCAG CACTGGACGA 
AGACAAGGAT CCCACCACGA GCAGGCACGA GACAGCTCCA GGCACTCAGC GTCCCAAGAG 
GGTCAGGACA CCATTCGTGC ACACCCGGGG TCAAGGAGAG GAGGAAGGCA GGGATCCCAC 
CATGAGCAAT CGGTAGATAG ATCTGGACAC TCAGGGTCCC ATCACAGCCA CACCACATCC 
CAGGGAAGGT CTGATGCCTC CCATGGGCAG TCAGGATCCA GAAGTGCAAG CAGACAAACT 
CGTAAGGACA AACAATCAGG AGACGGCTCC AGGCACTCAG GGTCACGTCA CCATGAAGCT 
GCCTCTTGGG CTGACAGCTC TAGACACTCA CAGGTGGGAC AGGAACAATC ATCGGGGTCC 
AGGACAAGCA GGCACCAGGG ATCCAGTGTT AGCCAGGACA GTGACAGTGA GAGACACTCA 
GACGACTCCG AGAGGTTGTC TGGGTCTGCT TCCAGAAACC ATCATGGATC TTCTCGGGAG 
CAGTCAAGAG ATGGCTCCAG ACACCCTGGG TTCCATCAAG AAGACAGAGC CAGTCACGGG 
CACTCTGCAG ACAGCTCCAG ACAATCAGGC ACTCATCACA CAGAGTCTTC CTCTCATGGA 
CAGGCTGTGT CATCCCATGA ACAGGCAAGA TCAAGTCCAG GAGAAAGACA TGGATCCCGC 
CACCAGCAGT CAGCAGACAG CTCCAGACAC TCAGGCATTG GGCACAGACA AGCTTCATCT 
GCAGTCAGAG ACAGTGGACA CCGAGGGTCC AGTGGTAGTC AGGTCACTAA CAGTGAGGGA 
CATTCAGAAG ACTCAGACAC ACAGTCAGTG TCAGCCCACG GACAAGCTGG GCCCCATCAG 
CAGAGCCACA AAGAGTCCGC ACGTGGCCAG TCAGGGGAAA GCTCTGGACG TTCAAGGTCT 
TTCCTCTACC AGGTGAGCTC TCATGAACAG TCTGAGTCCA CACACGGACA GACTGCACCC 
AGCACTGGAG GAAGACAAGG ATCCCGCCAT GAGCAGGCAC GAAACAGCTC TAGGCACTCA 
GCATCCCAAG ACGGTCAGGA CACCATTCGT GGACACCCGG GGTCAAGCAG AGGAGGAAGG 
CAGGGATCCT ACCACGAGCA ATCAGTAGAT AGGTCTGGAC ACTCAGGGTA CCATCACAGC 
CACACCACAC CCCAGGGAAG GTCTGATGCC TCCCATGGGC AGTCAGGACC CAGAAGTGCA 
AGCAGGCAAA CAAGAAATGA GGAACAATCA GGAGACGGCT CCAGGCACTC AGGGTCACGT 
CACCATGAAC CTTCCACTCG GGCCGGCAGC TCTAGACACT CACAGGTGGG CCAGGGAGAA 
TCAGCGGGGT CCAAGACAAG CAGGCGCCAG GGATCCAGTG TTAGTCAGGA CAGGGACAGT 
GAGGGACACT CAGAAGACTC TGAGAGGCGG TCTGAGTCGG CTTCCAGAAA CCATTATGGA 
TCTGCTCGGG AGCAGTCAAG ACATGGCTCC AGGAACCCCA GGTCCCATCA AGAAGATAGA 
GCCAGTCATG GGCACTCTGC AGAGAGCTCC AGACAATCAG GCACTCGTCA TGCAGAGACT 
TCCTCTGGTG GACAGGCTGC ATCATCCCAG GAACAGGCAA GGTCAAGTCC AGGAGAAAGA 
CATGGATCCC GCCACCAGCA GTCAGCAGAC AGCTCCACAG ACTCAGGCAC TGGGCGCAGA 
CAAGATTCAT CTGTAGTCGG AGACAGTGGA AACCGAGGGT CCAGTGGTAG CCAGGCCAGT 
GACAGCGAGG GACACTCAGA AGAGTCAGAC ACACAGTCAG TGTCAGCCCA CGGACAGGCT 
GGGCCCCATC AGCAGAGCCA CCAAGAGTCC ACACGTGGCC AGTCAGGGGA AAGGTCTGGA 
CGTTCAGGGT CTTTCCTCTA CCAGGTGAGC ACTCATGAAC AGTCTGAGTC CGCCCATGGA 
CGCACAGGGC CCAGCACTGG AGGAAGACAA AGATCCCGCC ACGAGCAGGC ACGAGACAGC 
TCCAGGCACT CAGCGTCCCA AGAGGGTCAG GACACCATTC GTGGACACCC AGGGTCAAGC 
AGAGGAGGAA GGCAGGGATC CCACTATGAG CAATCGGTAG ATAGTTCTGG ACACTCAGGG 
TCTCATCACA GCCACACCAC GTCCCAGGAA AGGTCTGATG TCTCCCGTGG GCAGTCAGGA 
TCCAGAAGTG TCAGCAGACA AACACGTAAT GAGAAACAAT CAGGAGACGG CTCCAGGCAC 
TCAGGGTCGC GTCACCATGA AGCTTCCTCT CGGGCCGACA GCTCTAGACA CTCGCAGGTG 
GGCCAGGGAC AATCATCAGG GCCCAGGACA AGCAGGAACC AGGGATCCAG TGTTAGCCAG 
GACAGTGACA GTCAGGGACA CTCAGAAGAC TCTGAGAGGT GGTCTGGGTC TGCTTCCAGA 
AACCATCTTG GATCTGCTTG GGAGCAGTCA AGAGATGGCT CCAGACACCC TGGGTCCCAT 
CACGAAGACA GAGCCGGTCA CGGGCACTCT GCAGACAGCT CCAGACAATC AGGCACTCGT 
CACACAGAGT CTTCCTCTCG TGGACAGGCT GCGTCATCCC ATGAACAGGC AAGATCAAGT 
GCAGGAGAAA GACATGGATC CCACCACCAG CTCCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ATGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTACAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCACAGGGAA AAGCTGGGCC CCATCAGCAG AGCCACAAAG AGTCCGCACG TGGCCAGTCA 
GGGGAAAGCT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCCACCC ATGGACAGTC TGCGCCCAGC ACTGGAGGAA GACAAGGATC CCATTATGAT 
CAGGCACAAG ACAGCTCCAG GCACTCAGCA TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGC CAAGCAGAGG AGGAAGACAG GGGTCCCACC AAGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCTCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CGTGGGCAGT CAGGATCCAG AAGTGCAAGC AGAAAAACAT ATGACAAGGA ACAATCAGGA 
GATGGCTCTA GGCACTCAGG GTCGCATCAT CATGAAGCTT CCTCTTGGGC CGACAGCTCT 
AGACACTCAC TGGTGGGCCA GGGACAATCA TCAGGGCCCA GGACAAGCAG GCCCCGGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GGACACTCAG AAGATTCTGA GAGGCGGTCT 
GGGTCTGCGT CCAGAAACCA TCATGGATCT GCTCAGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCAGGT CCCATCACGA AGACAGAGCC GGTCATGGGC ACTCTGCAGA GAGCTCCAGA 
CAATCAGGCA CTCATCATGC AGAGAATTCC TCTGGTGGAC AGGCTGCATC ATCCCATGAA 
CAGGCAAGAT CAAGTGCAGG AGAGAGACAC GGATCCCACC ACCAGCAGTC AGCAGACAGC 
TCCAGACACT CAGGCATTGG GCACGGACAA GCTTCATCTG CAGTCAGAGA CAGTGGACAC 
CGAGGGTCCA GTGGTAGTCA GGCCAGTGAC AGTGAGGGAC ATTCAGAAGA CTCAGACACA 
CAGTCAGTGT CAGCCCACGG ACAGGCTGGG CCCCATCAGC AGAGCCACCA AGAGTCCACA 
CGTGGCCGGT CAGCAGGAAG GTCTGGACGT TCAGGGTCTT TCCTCTACCA GGTGAGCACT 
CATGAACAGT CTGAGTCCGC CCATGGACGG ACCGGGACCA GCACTGGAGG AAGACAAGGA 
TCCCACCACA AGCAGGCACG AGACAGCTCC AGGCACTCAA CGTCCCAAGA GGGTCAGGAC 
ACCATTCATG GACACCCGGG GTCAAGCAGT GGAGGAAGGC AGGGATCCCA CTACGAGCAA 
TTGGTAGATA GATCTGGACA CTCAGGGTCT CATCACAGCC ACACCACATC CCAGGGAAGG 
TCTGATGCCT CCCATGGGCA CTCAGGATCC AGAAGTGCAA GCAGACAAAC TCGTAACGAT 
GAACAATCAG GAGACGGCTC CAGGCACTCA GGGTCGCGTC ACCATGAAGC TTCCTCTCGG 
GCCGACAGCT CTGGACACTC GCAGGTGGGC CAGGGACAAT CAGAGGGGCC CAGGACAAGC 
AGGAACTGGG GATCCAGTTT TAGCCAGGAC AGTGACAGTC AGGGACACTC AGAAGACTCT 
GAGAGGTGGT CTGGGTCTGC TTCCAGAAAC CATCATGGAT CTGCTCAGGA GCAGCTAAGA 
GATGGCTCCA GACACCCCAG GTCCCATCAA GAAGACAGAG CTGGTCATGG GCACTCTGCA 
GACAGCTCCA GACAATCAGG CACTCGTCAC ACACAGACTT CCTCTGGTGG ACAGGCTGCA 
TCATCCCATG AACAGGCAAG ATCAAGTGCA GGAGAAAGAC ATGGATCCCA CCACCAGCAG 
TCAGCAGACA GCTCCAGACA CTCAGGCATT GGGCACGGAC AAGCTTCATC TGCAGTCAGA 
GACAGTGGAC ACCGAGGGTA CAGTGGTAGT CAGGCCAGTG ACAATGAGGG ACATTCAGAA 
GACTCAGACA CACAGTCAGT GTCAGCCCAC GGACAGGCTG GGTCCCATCA GCAGAGCCAC 
CAAGAGTCCG CACGTGGCCG GTCAGGGGAA ACGTCTGGAC ATTCAGGATC TTTCCTCTAC 
CAGGTGAGCA CTCATGAACA GTCTGAGTCC TCCCATGGAT GGACGGGGCC CAGCACTAGA 
GGAAGACAAG GATCCCGCCA TGAGCAGGCA CAAGACAGCT CCAGGCACTC AGCATCCCAA 
GACGGTCAGG ACACCATTCG TGGACACCCG GGGTCAAGCA GAGGAGGAAG GCAGGGGTAC 
CACCACGAGC ATTCGGTAGA TAGCTCTGGA CACTCAGGGT CCCATCACAG CCACACCACA 
TCCCAGGGAA GGTCTGATGC CTCCCGTGGG CAGTCAGGAT CCAGAAGTGC AAGCAGAACA 
ACACGTAATG AGGAACAATC AGGAGACGGC TCCAGGCACT CAGGGTCGCG TCACCATGAA 
GCTTCCACTC ATGCCGACAT CTCTAGACAC TCACAGGCAG TCCAGGGACA ATCAGAGGGG 
TCCAGGAGAA GCAGGCGCCA GGGATCCAGT GTGAGCCAGG ACAGTGACAG TGAGGGACAT 
TCAGAAGACT CTGAGAGGTG GTCTGGGTCT GCTTCCAGAA ACCATCATGG ATCTGCTCAG 
GAGCAGCTAA GAGATGGCTC CAGACACCCC AGGTCCCATC AAGAAGACAG AGCTGGTCAT 
GGGCACTCTG CAGACAGCTC CAGACAATCA GGCACTCGTC ACACACAGAC TTCCTCTGGT 
GGACAGGCTG CATCATCCCA TGAACAGGCA AGATCAAGTG CAGGAGAAAG ACATGGATCC 
CACCACCAGC AGTCAGCAGA CAGCTCCAGA CACTCAGGCA TTGGGCACGG ACAAGCTTCA 
TCTGCAGTCA GAGACAGTGG ACACCGAGGG TACAGTGGTA GTCAGGCCAG TGACAATGAG 
GGACATTCAG AAGACTCAGA CACACAGTCA GTGTCAGCCC ACGGACAGGC TGGGTCCCAT 
CAGCAGAGCC ACCAAGAGTC CGCACGTGGC CGGTCAGGGG AAACGTCTGG ACATTCAGGA 
TCTTTCCTCT ACCAGGTGAG CACTCATGAA CAGTCTGAGT CCTCCCATGG ATGGACGGGG 
CCCAGCACTA GAGGAAGACA AGGATCCCGC CATGAGCAGG CACAAGACAG CTCCAGGCAC 
TCAGCATCCC AATACGGTCA GGACACCATT CGTGGACACC CGGGGTCAAG CAGAGGAGGA 
AGGCAGGGGT ACCACCACGA GCATTCGGTA GATAGCTCTG GACACTCAGG GTCCCATCAC 
AGCCACACCA CATCCCAGGG AAGGTCTGAT GCCTCCCGTG GGCAGTCAGG ATCCAGAAGT 
GCAAGCAGAA CAACACGTAA TGAGGAACAA TCAGGAGACA GCTCCAGGCA CTCAGTGTCA 
CGTCACCATG AAGCTTCCAC TCATGCCGAC ATCTCTAGAC ACTCACAGGC AGTCCAGGGA 
CAATCAGAGG GGTCCAGGAG AAGCAGGCGC CAGGGATCCA GTGTGAGCCA GGACAGTGAC 
AGTGAGGGAC ATTCAGAAGA CTCTGAGAGG TGGTCTGGGT CTGCTTCCAG AAACCATCGT 
GGATCTGTTC AGGAGCAGTC AAGGCACGGC TCCAGACACC CCAGGTCCCA TCACGAAGAC 
AGAGCCGGTC ACGGGCACTC TGCAGACCGC TCCAGACAAT CAGGCACTCG TCACGCAGAG 
ACTTCCTCTG GTGGACAGGC TGCATCATCC CATGAACAGG CAAGATCAAG TCCAGGAGAG 
AGACACGGAT CCCGCCACCA GCAGTCAGCA GACAGCTCCA GACACTCAGG CATTCCGCGT 
GGACAAGCTT CATCTGCAGT CAGAGACAGT AGACACTGGG GGTCCAGTGG TAGTCAGGCC 
AGTGATAGTG AGGGACATTC AGAAGAGTCA GACACACAGT CAGTGTCAGG CCATGGACAG 
GCTGGGCCCC ATCAGCAGAG CCACCAAGAG TCCGCACGTG ACCGGTCAGG GGGAAGGTCT 
GGACGTTCAG GGTCTTTCCT CTACCAGGTG AGCACTCATG AACAGTCTGA GTCTGCCCAT 
GGGCGGACCA GGACCAGCAC TGGACGAAGA CAAGGATCCC ACCACGAGCA GGCACGAGAC 
AGCTCCAGGC ACTCAGCGTC CCAAGAGGGT CAGGACACCA TTCGTGGACA CCCGGGGTCA 
AGCAGAAGAG GAAGGCAGGG ATCCCACTAC GAGCAATCGG TAGATAGGTC TGGACACTCA 
GGGTCCCATC ACAGCCACAC CACATCCCAG GGAAGGTCTG ATGCCTCCCG TGGGCAGTCA 
GGATCCAGAA GTGCCAGCAG ACAAACTCGT AATGACGAAC AATCAGGAGA TGGCTCCAGG 
CACTCATGGT CGCATCACCA TGAAGCTTCC ACTCAGGCGG ACAGCTCTAG ACACTCACAG 
TCCGGCCAGG GACAATCAGC GGGGCCCAGG ACAAGCAGGA ACCAGGGATC CAGTGTTAGC 
CAGGACAGTG ACAGTCAGGG ACACTCAGAA GACTCTGAGA GGTGGTCTGG GTCTGCTTCC 
AGAAACCATC GTGGATCTGC TCAGGAGCAG TCAAGAGATG GCTCCAGACA CCCCACGTCC 
CATCACGAAG ACAGAGCCGG TCACGGGCAC TCTGCAGAGA GCTCCAGACA ATCAGGCACT 
CATCATGCAG AGAATTCCTC TGGTGGACAG GCTGCATCAT CCCATGAACA GGCAAGATCA 
AGTGCAGGAG AGAGACATGG ATCCCACCAC CAGCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ACGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTCCAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCCCACGGAC AGGCTGGGCC CCATCAGCAG AGCCACCAAG AGTCCACACG TGGCCGGTCA 
GCAGGAAGGT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCTGCCC ATGGACGGGC TGGGCCCAGT ACTGGAGGAA GACAAGGATC CCGCCACGAG 
CAGGCACGAG ACAGCTCCAG GCACTCAGCG TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGT CAAGGAGAGG AGGAAGACAG GGATCCTACC ACGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCCCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CATGGGCAGT CAGGATCCAG AAGTGCAAGC AGAGAAACAC GTAATGAGGA ACAGTCAGGA 
GACGGCTCCA GGCACTCAGG GTCGCGTCAC CATGAAGCTT CCACTCAGGC TGACAGCTCT 
AGACACTCAC AGTCCGGCCA GGGTGAATCA GCGGGGTCCA GGAGAAGCAG GCGCCAGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GCATACCCAG AGGACTCTGA GAGGCGATCT 
GAGTCTGCTT CCAGAAACCA TCATGGATCT TCTCGGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCGGAT CCTCTCACCG CGATACAGCC AGTCATGTAC AGTCTTCACC TGTACAGTCA 
GACTCTAGTA CCGCTAAGGA ACATGGTCAC TTTAGTAGTC TTTCACAAGA TTCTGCGTAT 
CACTCAGGAA TACAGTCACG TGGCAGTCCT CACAGTTCTA GTTCTTATCA TTATCAATCT 
GAGGGCACTG AAAGGCAAAA AGGTCAATCA GGTTTAGTTT GGAGACATGG CAGCTATGGT 
AGTGCAGATT ATGATTATGG TGAATCCGGG TTTAGACACT CTCAGCACGG AAGTGTTAGT 
TACAATTCCA ATCCTGTTGT TTTCAAGGAA AGATCTGATA TCTGTAAAGC AAGTGCGTTT 
GGTAAAGATC ATCCAAGGTA TTATGCAACG TATATTAATA AGGACCCAGG TTTATGTGGC 
CATTCTAGTG ATATATCGAA ACAACTGGGA TTTAGTCAGT CACAGAGATA CTATTACTAT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLG ( NM_002016.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_002007.1 Homo sapiens filaggrin (FLG), mRNA.

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CloneID	OHu22241
Clone ID Related Accession (Same CDS sequence)	NM_002016.2 , NM_002016.1
Accession Version	NM_002016.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	filaggrin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356504.5. This sequence is a reference standard in the RefSeqGene project. On or before Feb 21, 2005 this sequence version replaced XM_378901.2, XM_048104.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L01090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTACTC TCCTGGAAAA CATCTTTGCC ATAATTAATC TTTTCAAGCA ATATTCAAAA 
AAAGATAAAA ACACTGACAC ATTGAGTAAA AAAGAGCTGA AGGAACTTCT GGAAAAGGAA 
TTTCGGCAAA TCCTGAAGAA TCCAGATGAC CCAGATATGG TTGATGTCTT CATGGATCAC 
TTGGATATAG ACCACAACAA GAAAATTGAC TTCACTGAGT TTCTTCTGAT GGTATTCAAG 
TTGGCTCAAG CATATTATGA GTCTACCAGA AAAGAGAATT TACCGATATC AGGACACAAG 
CACAGAAAGC ACAGTCATCA TGATAAACAT GAAGATAATA AACAGGAAGA AAACAAAGAA 
AACAGAAAAA GACCCTCAAG TCTGGAAAGA AGAAACAATA GAAAAGGGAA TAAGGGAAGA 
TCCAAGAGCC CAAGAGAAAC AGGGGGGAAA AGGCATGAAT CTAGTTCTGA AAAAAAAGAA 
AGAAAAGGAT ATTCACCTAC TCATAGAGAA GAAGAATATG GAAAAAACCA TCATAACTCA 
AGTAAAAAAG AGAAAAACAA GACTGAAAAT ACTAGATTAG GAGACAATAG GAAGAGGCTA 
AGTGAAAGAC TTGAAGAGAA AGAAGACAAT GAAGAAGGAG TATATGATTA TGAAAATACA 
GGAAGAATGA CTCAAAAATG GATACAATCA GGCCATATTG CCACATATTA CACAATCCAG 
GATGAAGCCT ATGACACCAC TGATAGTCTA TTAGAAGAAA ACAAAATATA TGAAAGATCA 
AGGTCATCTG ATGGCAAATC ATCATCTCAA GTGAACAGGT CAAGACATGA AAATACAAGC 
CAGGTACCAT TGCAGGAGTC CAGGACAAGA AAGCGTAGGG GATCCAGAGT TAGCCAGGAC 
AGGGACAGTG AGGGACACTC AGAAGACTCT GAGAGGCACT CTGGGTCGGC TTCCAGAAAC 
CATCATGGAT CTGCGTGGGA GCAGTCAAGA GATGGCTCCA GACACCCCAG GTCCCATGAT 
GAAGACAGAG CCAGTCATGG GCACTCTGCA GACAGCTCCA GACAATCAGG CACTCGTCAC 
GCAGAGACTT CCTCTCGTGG ACAGACTGCA TCATCCCATG AACAGGCAAG ATCAAGTCCA 
GGAGAAAGAC ATGGATCCGG CCACCAGCAG TCAGCAGACA GCTCCAGACA CTCAGCCACT 
GGGCGCGGGC AAGCTTCATC TGCAGTCAGC GATCGTGGAC ACCGGGGGTC TAGCGGTAGT 
CAGGCCAGTG ACAGTGAGGG ACATTCAGAA AACTCAGACA CACAATCAGT GTCAGGCCAC 
GGAAAGGCTG GGCTGAGACA GCAGAGCCAC CAAGAGTCCA CACGTGGCCG GTCAGGGGAA 
CGGTCTGGAC GTTCAGGGTC TTCCCTCTAC CAGGTGAGCA CTCATGAACA GCCTGACTCT 
GCCCATGGAC GGACCGGGAC CAGCACTGGA GGAAGACAAG GATCGCACCA CGAGCAGGCA 
CGAGACAGCT CCAGGCATTC AGCGTCCCAA GAGGGTCAGG ACACCATTCG TGGACACCCG 
GGGTCAAGCA GAGGAGGAAG GCAGGGATCC CACCACGAGC AATCGGTAAA TAGGTCTGGA 
CACTCAGGTT CCCATCACAG CCACACCACA TCCCAGGGAA GGTCTGATGC CTCCCATGGG 
CAGTCAGGAT CCAGAAGTGC AAGCAGACAA ACACGAAATG AGGAACAATC AGGAGACGGC 
ACCAGGCACT CAGGGTCACG TCATCATGAA GCTTCCTCTC AGGCTGACAG CTCTAGACAC 
TCACAGGTGG GCCAGGGACA ATCATCGGGG CCCAGGACAA GTAGGAACCA GGGATCCAGT 
GTTAGCCAGG ACAGTGACAG TCAGGGACAC TCAGAAGACT CTGAGAGGTG GTCTGGGTCT 
GCTTCCAGAA ACCATCATGG ATCTGCTCAG GAGCAGTCAA GAGATGGCTC CAGACACCCC 
AGGTCCCATC ACGAAGACAG AGCTGGTCAT GGGCACTCTG CAGACAGCTC CAGAAAATCA 
GGCACTCGTC ACACACAGAA TTCCTCTAGT GGACAGGCTG CGTCATCCCA TGAACAGGCA 
AGATCAAGTG CAGGAGAAAG ACATGGATCC CGCCACCAGC TCCAGTCAGC AGACAGCTCC 
AGACACTCAG GCACTGGGCA CGGACAAGCT TCATCTGCAG TCAGAGACAG TGGACACCGA 
GGGTCCAGTG GTAGTCAGGC CACTGACAGT GAGGGACATT CAGAAGACTC AGACACACAG 
TCAGTGTCAG GCCATGGACA GGCTGGTCAC CATCAGCAGA GCCACCAAGA GTCCGCACGT 
GACCGGTCAG GGGAAAGGTC TCGACGTTCA GGGTCTTTCC TCTACCAGGT GAGCACTCAT 
AAACAGTCTG AGTCCTCCCA TGGATGGACA GGGCCCAGCA CTGGAGTAAG ACAAGGATCC 
CACCATGAGC AGGCACGAGA CAACTCCAGG CACTCAGCAT CCCAAGATGG TCAGGACACC 
ATTCGTGGAC ACCCGGGGTC AAGCAGAAGA GGAAGGCAGG GGTCCCACCA CGAGCAATCG 
GTAGATAGGT CTGGACACTC AGGGTCCCAT CACAGCCACA CCACATCCCA GGGAAGGTCT 
GATGCCTCCC GTGGGCAGTC AGGATCCAGA AGTGCAAGCA GAACAACACG TAATGAGGAA 
CAATCAAGAG ACGGCTCCAG GCACTCAGGG TCACGTCACC ATGAAGCTTC CTCTCATGCC 
GACATCTCTA GACACTCACA GGCAGGCCAG GGACAATCAG AGGGGTCCAG GACAAGCAGG 
CGCCAGGGAT CCAGTGTTAG CCAGGACAGT GACAGTGAGG GACATTCAGA AGACTCTGAG 
AGGTGGTCTG GGTCTGCTTC CAGAAACCAT CGTGGATCTG CTCAGGAGCA GTCAAGACAT 
GGCTCCAGAC ACCCCAGGTC CCATCACGAA GACAGAGCCG GTCACGGGCA CTCTGCAGAC 
AGCTCCAGAC AATCAGGAAC TCCTCACGCA GAGACTTCCT CTGGTGGACA GGCTGCGTCA 
TCCCATGAAC AGGCAAGATC AAGTCCAGGA GAAAGACACG GATCCCGCCA CCAGCAGTCA 
GCAGACAGCT CCAGACACTC AGGCATTCCG CGCAGACAAG CTTCATCTGC AGTCAGAGAC 
AGTGGACACT GGGGGTCCAG TGGTAGTCAG GCCAGTGATA GTGAGGGACA TTCAGAGGAG 
TCAGACACAC AGTCAGTGTC AGGCCATGGA CAGGATGGGC CCCATCAGCA GAGCCACCAA 
GAGTCCGCAC GTGACTGGTC AGGGGGAAGG TCTGGACGTT CAGGGTCTTT CATCTACCAG 
GTGAGCACTC ATGAACAGTC TGAGTCTGCC CATGGGCGGA CCAGGACCAG CACTGGACGA 
AGACAAGGAT CCCACCACGA GCAGGCACGA GACAGCTCCA GGCACTCAGC GTCCCAAGAG 
GGTCAGGACA CCATTCGTGC ACACCCGGGG TCAAGGAGAG GAGGAAGGCA GGGATCCCAC 
CATGAGCAAT CGGTAGATAG ATCTGGACAC TCAGGGTCCC ATCACAGCCA CACCACATCC 
CAGGGAAGGT CTGATGCCTC CCATGGGCAG TCAGGATCCA GAAGTGCAAG CAGACAAACT 
CGTAAGGACA AACAATCAGG AGACGGCTCC AGGCACTCAG GGTCACGTCA CCATGAAGCT 
GCCTCTTGGG CTGACAGCTC TAGACACTCA CAGGTGGGAC AGGAACAATC ATCGGGGTCC 
AGGACAAGCA GGCACCAGGG ATCCAGTGTT AGCCAGGACA GTGACAGTGA GAGACACTCA 
GACGACTCCG AGAGGTTGTC TGGGTCTGCT TCCAGAAACC ATCATGGATC TTCTCGGGAG 
CAGTCAAGAG ATGGCTCCAG ACACCCTGGG TTCCATCAAG AAGACAGAGC CAGTCACGGG 
CACTCTGCAG ACAGCTCCAG ACAATCAGGC ACTCATCACA CAGAGTCTTC CTCTCATGGA 
CAGGCTGTGT CATCCCATGA ACAGGCAAGA TCAAGTCCAG GAGAAAGACA TGGATCCCGC 
CACCAGCAGT CAGCAGACAG CTCCAGACAC TCAGGCATTG GGCACAGACA AGCTTCATCT 
GCAGTCAGAG ACAGTGGACA CCGAGGGTCC AGTGGTAGTC AGGTCACTAA CAGTGAGGGA 
CATTCAGAAG ACTCAGACAC ACAGTCAGTG TCAGCCCACG GACAAGCTGG GCCCCATCAG 
CAGAGCCACA AAGAGTCCGC ACGTGGCCAG TCAGGGGAAA GCTCTGGACG TTCAAGGTCT 
TTCCTCTACC AGGTGAGCTC TCATGAACAG TCTGAGTCCA CACACGGACA GACTGCACCC 
AGCACTGGAG GAAGACAAGG ATCCCGCCAT GAGCAGGCAC GAAACAGCTC TAGGCACTCA 
GCATCCCAAG ACGGTCAGGA CACCATTCGT GGACACCCGG GGTCAAGCAG AGGAGGAAGG 
CAGGGATCCT ACCACGAGCA ATCAGTAGAT AGGTCTGGAC ACTCAGGGTA CCATCACAGC 
CACACCACAC CCCAGGGAAG GTCTGATGCC TCCCATGGGC AGTCAGGACC CAGAAGTGCA 
AGCAGGCAAA CAAGAAATGA GGAACAATCA GGAGACGGCT CCAGGCACTC AGGGTCACGT 
CACCATGAAC CTTCCACTCG GGCCGGCAGC TCTAGACACT CACAGGTGGG CCAGGGAGAA 
TCAGCGGGGT CCAAGACAAG CAGGCGCCAG GGATCCAGTG TTAGTCAGGA CAGGGACAGT 
GAGGGACACT CAGAAGACTC TGAGAGGCGG TCTGAGTCGG CTTCCAGAAA CCATTATGGA 
TCTGCTCGGG AGCAGTCAAG ACATGGCTCC AGGAACCCCA GGTCCCATCA AGAAGATAGA 
GCCAGTCATG GGCACTCTGC AGAGAGCTCC AGACAATCAG GCACTCGTCA TGCAGAGACT 
TCCTCTGGTG GACAGGCTGC ATCATCCCAG GAACAGGCAA GGTCAAGTCC AGGAGAAAGA 
CATGGATCCC GCCACCAGCA GTCAGCAGAC AGCTCCACAG ACTCAGGCAC TGGGCGCAGA 
CAAGATTCAT CTGTAGTCGG AGACAGTGGA AACCGAGGGT CCAGTGGTAG CCAGGCCAGT 
GACAGCGAGG GACACTCAGA AGAGTCAGAC ACACAGTCAG TGTCAGCCCA CGGACAGGCT 
GGGCCCCATC AGCAGAGCCA CCAAGAGTCC ACACGTGGCC AGTCAGGGGA AAGGTCTGGA 
CGTTCAGGGT CTTTCCTCTA CCAGGTGAGC ACTCATGAAC AGTCTGAGTC CGCCCATGGA 
CGCACAGGGC CCAGCACTGG AGGAAGACAA AGATCCCGCC ACGAGCAGGC ACGAGACAGC 
TCCAGGCACT CAGCGTCCCA AGAGGGTCAG GACACCATTC GTGGACACCC AGGGTCAAGC 
AGAGGAGGAA GGCAGGGATC CCACTATGAG CAATCGGTAG ATAGTTCTGG ACACTCAGGG 
TCTCATCACA GCCACACCAC GTCCCAGGAA AGGTCTGATG TCTCCCGTGG GCAGTCAGGA 
TCCAGAAGTG TCAGCAGACA AACACGTAAT GAGAAACAAT CAGGAGACGG CTCCAGGCAC 
TCAGGGTCGC GTCACCATGA AGCTTCCTCT CGGGCCGACA GCTCTAGACA CTCGCAGGTG 
GGCCAGGGAC AATCATCAGG GCCCAGGACA AGCAGGAACC AGGGATCCAG TGTTAGCCAG 
GACAGTGACA GTCAGGGACA CTCAGAAGAC TCTGAGAGGT GGTCTGGGTC TGCTTCCAGA 
AACCATCTTG GATCTGCTTG GGAGCAGTCA AGAGATGGCT CCAGACACCC TGGGTCCCAT 
CACGAAGACA GAGCCGGTCA CGGGCACTCT GCAGACAGCT CCAGACAATC AGGCACTCGT 
CACACAGAGT CTTCCTCTCG TGGACAGGCT GCGTCATCCC ATGAACAGGC AAGATCAAGT 
GCAGGAGAAA GACATGGATC CCACCACCAG CTCCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ATGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTACAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCACAGGGAA AAGCTGGGCC CCATCAGCAG AGCCACAAAG AGTCCGCACG TGGCCAGTCA 
GGGGAAAGCT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCCACCC ATGGACAGTC TGCGCCCAGC ACTGGAGGAA GACAAGGATC CCATTATGAT 
CAGGCACAAG ACAGCTCCAG GCACTCAGCA TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGC CAAGCAGAGG AGGAAGACAG GGGTCCCACC AAGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCTCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CGTGGGCAGT CAGGATCCAG AAGTGCAAGC AGAAAAACAT ATGACAAGGA ACAATCAGGA 
GATGGCTCTA GGCACTCAGG GTCGCATCAT CATGAAGCTT CCTCTTGGGC CGACAGCTCT 
AGACACTCAC TGGTGGGCCA GGGACAATCA TCAGGGCCCA GGACAAGCAG GCCCCGGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GGACACTCAG AAGATTCTGA GAGGCGGTCT 
GGGTCTGCGT CCAGAAACCA TCATGGATCT GCTCAGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCAGGT CCCATCACGA AGACAGAGCC GGTCATGGGC ACTCTGCAGA GAGCTCCAGA 
CAATCAGGCA CTCATCATGC AGAGAATTCC TCTGGTGGAC AGGCTGCATC ATCCCATGAA 
CAGGCAAGAT CAAGTGCAGG AGAGAGACAC GGATCCCACC ACCAGCAGTC AGCAGACAGC 
TCCAGACACT CAGGCATTGG GCACGGACAA GCTTCATCTG CAGTCAGAGA CAGTGGACAC 
CGAGGGTCCA GTGGTAGTCA GGCCAGTGAC AGTGAGGGAC ATTCAGAAGA CTCAGACACA 
CAGTCAGTGT CAGCCCACGG ACAGGCTGGG CCCCATCAGC AGAGCCACCA AGAGTCCACA 
CGTGGCCGGT CAGCAGGAAG GTCTGGACGT TCAGGGTCTT TCCTCTACCA GGTGAGCACT 
CATGAACAGT CTGAGTCCGC CCATGGACGG ACCGGGACCA GCACTGGAGG AAGACAAGGA 
TCCCACCACA AGCAGGCACG AGACAGCTCC AGGCACTCAA CGTCCCAAGA GGGTCAGGAC 
ACCATTCATG GACACCCGGG GTCAAGCAGT GGAGGAAGGC AGGGATCCCA CTACGAGCAA 
TTGGTAGATA GATCTGGACA CTCAGGGTCT CATCACAGCC ACACCACATC CCAGGGAAGG 
TCTGATGCCT CCCATGGGCA CTCAGGATCC AGAAGTGCAA GCAGACAAAC TCGTAACGAT 
GAACAATCAG GAGACGGCTC CAGGCACTCA GGGTCGCGTC ACCATGAAGC TTCCTCTCGG 
GCCGACAGCT CTGGACACTC GCAGGTGGGC CAGGGACAAT CAGAGGGGCC CAGGACAAGC 
AGGAACTGGG GATCCAGTTT TAGCCAGGAC AGTGACAGTC AGGGACACTC AGAAGACTCT 
GAGAGGTGGT CTGGGTCTGC TTCCAGAAAC CATCATGGAT CTGCTCAGGA GCAGCTAAGA 
GATGGCTCCA GACACCCCAG GTCCCATCAA GAAGACAGAG CTGGTCATGG GCACTCTGCA 
GACAGCTCCA GACAATCAGG CACTCGTCAC ACACAGACTT CCTCTGGTGG ACAGGCTGCA 
TCATCCCATG AACAGGCAAG ATCAAGTGCA GGAGAAAGAC ATGGATCCCA CCACCAGCAG 
TCAGCAGACA GCTCCAGACA CTCAGGCATT GGGCACGGAC AAGCTTCATC TGCAGTCAGA 
GACAGTGGAC ACCGAGGGTA CAGTGGTAGT CAGGCCAGTG ACAATGAGGG ACATTCAGAA 
GACTCAGACA CACAGTCAGT GTCAGCCCAC GGACAGGCTG GGTCCCATCA GCAGAGCCAC 
CAAGAGTCCG CACGTGGCCG GTCAGGGGAA ACGTCTGGAC ATTCAGGATC TTTCCTCTAC 
CAGGTGAGCA CTCATGAACA GTCTGAGTCC TCCCATGGAT GGACGGGGCC CAGCACTAGA 
GGAAGACAAG GATCCCGCCA TGAGCAGGCA CAAGACAGCT CCAGGCACTC AGCATCCCAA 
GACGGTCAGG ACACCATTCG TGGACACCCG GGGTCAAGCA GAGGAGGAAG GCAGGGGTAC 
CACCACGAGC ATTCGGTAGA TAGCTCTGGA CACTCAGGGT CCCATCACAG CCACACCACA 
TCCCAGGGAA GGTCTGATGC CTCCCGTGGG CAGTCAGGAT CCAGAAGTGC AAGCAGAACA 
ACACGTAATG AGGAACAATC AGGAGACGGC TCCAGGCACT CAGGGTCGCG TCACCATGAA 
GCTTCCACTC ATGCCGACAT CTCTAGACAC TCACAGGCAG TCCAGGGACA ATCAGAGGGG 
TCCAGGAGAA GCAGGCGCCA GGGATCCAGT GTGAGCCAGG ACAGTGACAG TGAGGGACAT 
TCAGAAGACT CTGAGAGGTG GTCTGGGTCT GCTTCCAGAA ACCATCATGG ATCTGCTCAG 
GAGCAGCTAA GAGATGGCTC CAGACACCCC AGGTCCCATC AAGAAGACAG AGCTGGTCAT 
GGGCACTCTG CAGACAGCTC CAGACAATCA GGCACTCGTC ACACACAGAC TTCCTCTGGT 
GGACAGGCTG CATCATCCCA TGAACAGGCA AGATCAAGTG CAGGAGAAAG ACATGGATCC 
CACCACCAGC AGTCAGCAGA CAGCTCCAGA CACTCAGGCA TTGGGCACGG ACAAGCTTCA 
TCTGCAGTCA GAGACAGTGG ACACCGAGGG TACAGTGGTA GTCAGGCCAG TGACAATGAG 
GGACATTCAG AAGACTCAGA CACACAGTCA GTGTCAGCCC ACGGACAGGC TGGGTCCCAT 
CAGCAGAGCC ACCAAGAGTC CGCACGTGGC CGGTCAGGGG AAACGTCTGG ACATTCAGGA 
TCTTTCCTCT ACCAGGTGAG CACTCATGAA CAGTCTGAGT CCTCCCATGG ATGGACGGGG 
CCCAGCACTA GAGGAAGACA AGGATCCCGC CATGAGCAGG CACAAGACAG CTCCAGGCAC 
TCAGCATCCC AATACGGTCA GGACACCATT CGTGGACACC CGGGGTCAAG CAGAGGAGGA 
AGGCAGGGGT ACCACCACGA GCATTCGGTA GATAGCTCTG GACACTCAGG GTCCCATCAC 
AGCCACACCA CATCCCAGGG AAGGTCTGAT GCCTCCCGTG GGCAGTCAGG ATCCAGAAGT 
GCAAGCAGAA CAACACGTAA TGAGGAACAA TCAGGAGACA GCTCCAGGCA CTCAGTGTCA 
CGTCACCATG AAGCTTCCAC TCATGCCGAC ATCTCTAGAC ACTCACAGGC AGTCCAGGGA 
CAATCAGAGG GGTCCAGGAG AAGCAGGCGC CAGGGATCCA GTGTGAGCCA GGACAGTGAC 
AGTGAGGGAC ATTCAGAAGA CTCTGAGAGG TGGTCTGGGT CTGCTTCCAG AAACCATCGT 
GGATCTGTTC AGGAGCAGTC AAGGCACGGC TCCAGACACC CCAGGTCCCA TCACGAAGAC 
AGAGCCGGTC ACGGGCACTC TGCAGACCGC TCCAGACAAT CAGGCACTCG TCACGCAGAG 
ACTTCCTCTG GTGGACAGGC TGCATCATCC CATGAACAGG CAAGATCAAG TCCAGGAGAG 
AGACACGGAT CCCGCCACCA GCAGTCAGCA GACAGCTCCA GACACTCAGG CATTCCGCGT 
GGACAAGCTT CATCTGCAGT CAGAGACAGT AGACACTGGG GGTCCAGTGG TAGTCAGGCC 
AGTGATAGTG AGGGACATTC AGAAGAGTCA GACACACAGT CAGTGTCAGG CCATGGACAG 
GCTGGGCCCC ATCAGCAGAG CCACCAAGAG TCCGCACGTG ACCGGTCAGG GGGAAGGTCT 
GGACGTTCAG GGTCTTTCCT CTACCAGGTG AGCACTCATG AACAGTCTGA GTCTGCCCAT 
GGGCGGACCA GGACCAGCAC TGGACGAAGA CAAGGATCCC ACCACGAGCA GGCACGAGAC 
AGCTCCAGGC ACTCAGCGTC CCAAGAGGGT CAGGACACCA TTCGTGGACA CCCGGGGTCA 
AGCAGAAGAG GAAGGCAGGG ATCCCACTAC GAGCAATCGG TAGATAGGTC TGGACACTCA 
GGGTCCCATC ACAGCCACAC CACATCCCAG GGAAGGTCTG ATGCCTCCCG TGGGCAGTCA 
GGATCCAGAA GTGCCAGCAG ACAAACTCGT AATGACGAAC AATCAGGAGA TGGCTCCAGG 
CACTCATGGT CGCATCACCA TGAAGCTTCC ACTCAGGCGG ACAGCTCTAG ACACTCACAG 
TCCGGCCAGG GACAATCAGC GGGGCCCAGG ACAAGCAGGA ACCAGGGATC CAGTGTTAGC 
CAGGACAGTG ACAGTCAGGG ACACTCAGAA GACTCTGAGA GGTGGTCTGG GTCTGCTTCC 
AGAAACCATC GTGGATCTGC TCAGGAGCAG TCAAGAGATG GCTCCAGACA CCCCACGTCC 
CATCACGAAG ACAGAGCCGG TCACGGGCAC TCTGCAGAGA GCTCCAGACA ATCAGGCACT 
CATCATGCAG AGAATTCCTC TGGTGGACAG GCTGCATCAT CCCATGAACA GGCAAGATCA 
AGTGCAGGAG AGAGACATGG ATCCCACCAC CAGCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ACGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTCCAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCCCACGGAC AGGCTGGGCC CCATCAGCAG AGCCACCAAG AGTCCACACG TGGCCGGTCA 
GCAGGAAGGT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCTGCCC ATGGACGGGC TGGGCCCAGT ACTGGAGGAA GACAAGGATC CCGCCACGAG 
CAGGCACGAG ACAGCTCCAG GCACTCAGCG TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGT CAAGGAGAGG AGGAAGACAG GGATCCTACC ACGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCCCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CATGGGCAGT CAGGATCCAG AAGTGCAAGC AGAGAAACAC GTAATGAGGA ACAGTCAGGA 
GACGGCTCCA GGCACTCAGG GTCGCGTCAC CATGAAGCTT CCACTCAGGC TGACAGCTCT 
AGACACTCAC AGTCCGGCCA GGGTGAATCA GCGGGGTCCA GGAGAAGCAG GCGCCAGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GCATACCCAG AGGACTCTGA GAGGCGATCT 
GAGTCTGCTT CCAGAAACCA TCATGGATCT TCTCGGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCGGAT CCTCTCACCG CGATACAGCC AGTCATGTAC AGTCTTCACC TGTACAGTCA 
GACTCTAGTA CCGCTAAGGA ACATGGTCAC TTTAGTAGTC TTTCACAAGA TTCTGCGTAT 
CACTCAGGAA TACAGTCACG TGGCAGTCCT CACAGTTCTA GTTCTTATCA TTATCAATCT 
GAGGGCACTG AAAGGCAAAA AGGTCAATCA GGTTTAGTTT GGAGACATGG CAGCTATGGT 
AGTGCAGATT ATGATTATGG TGAATCCGGG TTTAGACACT CTCAGCACGG AAGTGTTAGT 
TACAATTCCA ATCCTGTTGT TTTCAAGGAA AGATCTGATA TCTGTAAAGC AAGTGCGTTT 
GGTAAAGATC ATCCAAGGTA TTATGCAACG TATATTAATA AGGACCCAGG TTTATGTGGC 
CATTCTAGTG ATATATCGAA ACAACTGGGA TTTAGTCAGT CACAGAGATA CTATTACTAT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002007.1
CDS	37..12222
Translation	MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQILKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHRKHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKERKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYENTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRHENTSQVPLQESRTRKRRGSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSRHPRSHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSADSSRHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSHQESTRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRNEEQSGDGTRHSGSRHHEASSQADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSADSSRKSGTRHTQNSSSGQAASSHEQARSSAGERHGSRHQLQSADSSRHSGTGHGQASSAVRDSGHRGSSGSQATDSEGHSEDSDTQSVSGHGQAGHHQQSHQESARDRSGERSRRSGSFLYQVSTHKQSESSHGWTGPSTGVRQGSHHEQARDNSRHSASQDGQDTIRGHPGSSRRGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSRDGSRHSGSRHHEASSHADISRHSQAGQGQSEGSRTSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSAQEQSRHGSRHPRSHHEDRAGHGHSADSSRQSGTPHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRRQASSAVRDSGHWGSSGSQASDSEGHSEESDTQSVSGHGQDGPHQQSHQESARDWSGGRSGRSGSFIYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRAHPGSRRGGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRKDKQSGDGSRHSGSRHHEAASWADSSRHSQVGQEQSSGSRTSRHQGSSVSQDSDSERHSDDSERLSGSASRNHHGSSREQSRDGSRHPGFHQEDRASHGHSADSSRQSGTHHTESSSHGQAVSSHEQARSSPGERHGSRHQQSADSSRHSGIGHRQASSAVRDSGHRGSSGSQVTNSEGHSEDSDTQSVSAHGQAGPHQQSHKESARGQSGESSGRSRSFLYQVSSHEQSESTHGQTAPSTGGRQGSRHEQARNSSRHSASQDGQDTIRGHPGSSRGGRQGSYHEQSVDRSGHSGYHHSHTTPQGRSDASHGQSGPRSASRQTRNEEQSGDGSRHSGSRHHEPSTRAGSSRHSQVGQGESAGSKTSRRQGSSVSQDRDSEGHSEDSERRSESASRNHYGSAREQSRHGSRNPRSHQEDRASHGHSAESSRQSGTRHAETSSGGQAASSQEQARSSPGERHGSRHQQSADSSTDSGTGRRQDSSVVGDSGNRGSSGSQASDSEGHSEESDTQSVSAHGQAGPHQQSHQESTRGQSGERSGRSGSFLYQVSTHEQSESAHGRTGPSTGGRQRSRHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHYEQSVDSSGHSGSHHSHTTSQERSDVSRGQSGSRSVSRQTRNEKQSGDGSRHSGSRHHEASSRADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHLGSAWEQSRDGSRHPGSHHEDRAGHGHSADSSRQSGTRHTESSSRGQAASSHEQARSSAGERHGSHHQLQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDSEGHSEDSDTQSVSAQGKAGPHQQSHKESARGQSGESSGRSGSFLYQVSTHEQSESTHGQSAPSTGGRQGSHYDQAQDSSRHSASQEGQDTIRGHPGPSRGGRQGSHQEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRKTYDKEQSGDGSRHSGSHHHEASSWADSSRHSLVGQGQSSGPRTSRPRGSSVSQDSDSEGHSEDSERRSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRTGTSTGGRQGSHHKQARDSSRHSTSQEGQDTIHGHPGSSSGGRQGSHYEQLVDRSGHSGSHHSHTTSQGRSDASHGHSGSRSASRQTRNDEQSGDGSRHSGSRHHEASSRADSSGHSQVGQGQSEGPRTSRNWGSSFSQDSDSQGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQDGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDGSRHSGSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQYGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDSSRHSVSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSVQEQSRHGSRHPRSHHEDRAGHGHSADRSRQSGTRHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRGQASSAVRDSRHWGSSGSQASDSEGHSEESDTQSVSGHGQAGPHQQSHQESARDRSGGRSGRSGSFLYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRRGRQGSHYEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRQTRNDEQSGDGSRHSWSHHHEASTQADSSRHSQSGQGQSAGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHRGSAQEQSRDGSRHPTSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRAGPSTGGRQGSRHEQARDSSRHSASQEGQDTIRGHPGSRRGGRQGSYHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRETRNEEQSGDGSRHSGSRHHEASTQADSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE

Target ORF information:

RefSeq Version	NM_002016.1
Organism	Homo sapiens(human)
Definition	Homo sapiens filaggrin (FLG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002016.1

ORF Insert Sequence:

ATGTCTACTC TCCTGGAAAA CATCTTTGCC ATAATTAATC TTTTCAAGCA ATATTCAAAA 
AAAGATAAAA ACACTGACAC ATTGAGTAAA AAAGAGCTGA AGGAACTTCT GGAAAAGGAA 
TTTCGGCAAA TCCTGAAGAA TCCAGATGAC CCAGATATGG TTGATGTCTT CATGGATCAC 
TTGGATATAG ACCACAACAA GAAAATTGAC TTCACTGAGT TTCTTCTGAT GGTATTCAAG 
TTGGCTCAAG CATATTATGA GTCTACCAGA AAAGAGAATT TACCGATATC AGGACACAAG 
CACAGAAAGC ACAGTCATCA TGATAAACAT GAAGATAATA AACAGGAAGA AAACAAAGAA 
AACAGAAAAA GACCCTCAAG TCTGGAAAGA AGAAACAATA GAAAAGGGAA TAAGGGAAGA 
TCCAAGAGCC CAAGAGAAAC AGGGGGGAAA AGGCATGAAT CTAGTTCTGA AAAAAAAGAA 
AGAAAAGGAT ATTCACCTAC TCATAGAGAA GAAGAATATG GAAAAAACCA TCATAACTCA 
AGTAAAAAAG AGAAAAACAA GACTGAAAAT ACTAGATTAG GAGACAATAG GAAGAGGCTA 
AGTGAAAGAC TTGAAGAGAA AGAAGACAAT GAAGAAGGAG TATATGATTA TGAAAATACA 
GGAAGAATGA CTCAAAAATG GATACAATCA GGCCATATTG CCACATATTA CACAATCCAG 
GATGAAGCCT ATGACACCAC TGATAGTCTA TTAGAAGAAA ACAAAATATA TGAAAGATCA 
AGGTCATCTG ATGGCAAATC ATCATCTCAA GTGAACAGGT CAAGACATGA AAATACAAGC 
CAGGTACCAT TGCAGGAGTC CAGGACAAGA AAGCGTAGGG GATCCAGAGT TAGCCAGGAC 
AGGGACAGTG AGGGACACTC AGAAGACTCT GAGAGGCACT CTGGGTCGGC TTCCAGAAAC 
CATCATGGAT CTGCGTGGGA GCAGTCAAGA GATGGCTCCA GACACCCCAG GTCCCATGAT 
GAAGACAGAG CCAGTCATGG GCACTCTGCA GACAGCTCCA GACAATCAGG CACTCGTCAC 
GCAGAGACTT CCTCTCGTGG ACAGACTGCA TCATCCCATG AACAGGCAAG ATCAAGTCCA 
GGAGAAAGAC ATGGATCCGG CCACCAGCAG TCAGCAGACA GCTCCAGACA CTCAGCCACT 
GGGCGCGGGC AAGCTTCATC TGCAGTCAGC GATCGTGGAC ACCGGGGGTC TAGCGGTAGT 
CAGGCCAGTG ACAGTGAGGG ACATTCAGAA AACTCAGACA CACAATCAGT GTCAGGCCAC 
GGAAAGGCTG GGCTGAGACA GCAGAGCCAC CAAGAGTCCA CACGTGGCCG GTCAGGGGAA 
CGGTCTGGAC GTTCAGGGTC TTCCCTCTAC CAGGTGAGCA CTCATGAACA GCCTGACTCT 
GCCCATGGAC GGACCGGGAC CAGCACTGGA GGAAGACAAG GATCGCACCA CGAGCAGGCA 
CGAGACAGCT CCAGGCATTC AGCGTCCCAA GAGGGTCAGG ACACCATTCG TGGACACCCG 
GGGTCAAGCA GAGGAGGAAG GCAGGGATCC CACCACGAGC AATCGGTAAA TAGGTCTGGA 
CACTCAGGTT CCCATCACAG CCACACCACA TCCCAGGGAA GGTCTGATGC CTCCCATGGG 
CAGTCAGGAT CCAGAAGTGC AAGCAGACAA ACACGAAATG AGGAACAATC AGGAGACGGC 
ACCAGGCACT CAGGGTCACG TCATCATGAA GCTTCCTCTC AGGCTGACAG CTCTAGACAC 
TCACAGGTGG GCCAGGGACA ATCATCGGGG CCCAGGACAA GTAGGAACCA GGGATCCAGT 
GTTAGCCAGG ACAGTGACAG TCAGGGACAC TCAGAAGACT CTGAGAGGTG GTCTGGGTCT 
GCTTCCAGAA ACCATCATGG ATCTGCTCAG GAGCAGTCAA GAGATGGCTC CAGACACCCC 
AGGTCCCATC ACGAAGACAG AGCTGGTCAT GGGCACTCTG CAGACAGCTC CAGAAAATCA 
GGCACTCGTC ACACACAGAA TTCCTCTAGT GGACAGGCTG CGTCATCCCA TGAACAGGCA 
AGATCAAGTG CAGGAGAAAG ACATGGATCC CGCCACCAGC TCCAGTCAGC AGACAGCTCC 
AGACACTCAG GCACTGGGCA CGGACAAGCT TCATCTGCAG TCAGAGACAG TGGACACCGA 
GGGTCCAGTG GTAGTCAGGC CACTGACAGT GAGGGACATT CAGAAGACTC AGACACACAG 
TCAGTGTCAG GCCATGGACA GGCTGGTCAC CATCAGCAGA GCCACCAAGA GTCCGCACGT 
GACCGGTCAG GGGAAAGGTC TCGACGTTCA GGGTCTTTCC TCTACCAGGT GAGCACTCAT 
AAACAGTCTG AGTCCTCCCA TGGATGGACA GGGCCCAGCA CTGGAGTAAG ACAAGGATCC 
CACCATGAGC AGGCACGAGA CAACTCCAGG CACTCAGCAT CCCAAGATGG TCAGGACACC 
ATTCGTGGAC ACCCGGGGTC AAGCAGAAGA GGAAGGCAGG GGTCCCACCA CGAGCAATCG 
GTAGATAGGT CTGGACACTC AGGGTCCCAT CACAGCCACA CCACATCCCA GGGAAGGTCT 
GATGCCTCCC GTGGGCAGTC AGGATCCAGA AGTGCAAGCA GAACAACACG TAATGAGGAA 
CAATCAAGAG ACGGCTCCAG GCACTCAGGG TCACGTCACC ATGAAGCTTC CTCTCATGCC 
GACATCTCTA GACACTCACA GGCAGGCCAG GGACAATCAG AGGGGTCCAG GACAAGCAGG 
CGCCAGGGAT CCAGTGTTAG CCAGGACAGT GACAGTGAGG GACATTCAGA AGACTCTGAG 
AGGTGGTCTG GGTCTGCTTC CAGAAACCAT CGTGGATCTG CTCAGGAGCA GTCAAGACAT 
GGCTCCAGAC ACCCCAGGTC CCATCACGAA GACAGAGCCG GTCACGGGCA CTCTGCAGAC 
AGCTCCAGAC AATCAGGAAC TCCTCACGCA GAGACTTCCT CTGGTGGACA GGCTGCGTCA 
TCCCATGAAC AGGCAAGATC AAGTCCAGGA GAAAGACACG GATCCCGCCA CCAGCAGTCA 
GCAGACAGCT CCAGACACTC AGGCATTCCG CGCAGACAAG CTTCATCTGC AGTCAGAGAC 
AGTGGACACT GGGGGTCCAG TGGTAGTCAG GCCAGTGATA GTGAGGGACA TTCAGAGGAG 
TCAGACACAC AGTCAGTGTC AGGCCATGGA CAGGATGGGC CCCATCAGCA GAGCCACCAA 
GAGTCCGCAC GTGACTGGTC AGGGGGAAGG TCTGGACGTT CAGGGTCTTT CATCTACCAG 
GTGAGCACTC ATGAACAGTC TGAGTCTGCC CATGGGCGGA CCAGGACCAG CACTGGACGA 
AGACAAGGAT CCCACCACGA GCAGGCACGA GACAGCTCCA GGCACTCAGC GTCCCAAGAG 
GGTCAGGACA CCATTCGTGC ACACCCGGGG TCAAGGAGAG GAGGAAGGCA GGGATCCCAC 
CATGAGCAAT CGGTAGATAG ATCTGGACAC TCAGGGTCCC ATCACAGCCA CACCACATCC 
CAGGGAAGGT CTGATGCCTC CCATGGGCAG TCAGGATCCA GAAGTGCAAG CAGACAAACT 
CGTAAGGACA AACAATCAGG AGACGGCTCC AGGCACTCAG GGTCACGTCA CCATGAAGCT 
GCCTCTTGGG CTGACAGCTC TAGACACTCA CAGGTGGGAC AGGAACAATC ATCGGGGTCC 
AGGACAAGCA GGCACCAGGG ATCCAGTGTT AGCCAGGACA GTGACAGTGA GAGACACTCA 
GACGACTCCG AGAGGTTGTC TGGGTCTGCT TCCAGAAACC ATCATGGATC TTCTCGGGAG 
CAGTCAAGAG ATGGCTCCAG ACACCCTGGG TTCCATCAAG AAGACAGAGC CAGTCACGGG 
CACTCTGCAG ACAGCTCCAG ACAATCAGGC ACTCATCACA CAGAGTCTTC CTCTCATGGA 
CAGGCTGTGT CATCCCATGA ACAGGCAAGA TCAAGTCCAG GAGAAAGACA TGGATCCCGC 
CACCAGCAGT CAGCAGACAG CTCCAGACAC TCAGGCATTG GGCACAGACA AGCTTCATCT 
GCAGTCAGAG ACAGTGGACA CCGAGGGTCC AGTGGTAGTC AGGTCACTAA CAGTGAGGGA 
CATTCAGAAG ACTCAGACAC ACAGTCAGTG TCAGCCCACG GACAAGCTGG GCCCCATCAG 
CAGAGCCACA AAGAGTCCGC ACGTGGCCAG TCAGGGGAAA GCTCTGGACG TTCAAGGTCT 
TTCCTCTACC AGGTGAGCTC TCATGAACAG TCTGAGTCCA CACACGGACA GACTGCACCC 
AGCACTGGAG GAAGACAAGG ATCCCGCCAT GAGCAGGCAC GAAACAGCTC TAGGCACTCA 
GCATCCCAAG ACGGTCAGGA CACCATTCGT GGACACCCGG GGTCAAGCAG AGGAGGAAGG 
CAGGGATCCT ACCACGAGCA ATCAGTAGAT AGGTCTGGAC ACTCAGGGTA CCATCACAGC 
CACACCACAC CCCAGGGAAG GTCTGATGCC TCCCATGGGC AGTCAGGACC CAGAAGTGCA 
AGCAGGCAAA CAAGAAATGA GGAACAATCA GGAGACGGCT CCAGGCACTC AGGGTCACGT 
CACCATGAAC CTTCCACTCG GGCCGGCAGC TCTAGACACT CACAGGTGGG CCAGGGAGAA 
TCAGCGGGGT CCAAGACAAG CAGGCGCCAG GGATCCAGTG TTAGTCAGGA CAGGGACAGT 
GAGGGACACT CAGAAGACTC TGAGAGGCGG TCTGAGTCGG CTTCCAGAAA CCATTATGGA 
TCTGCTCGGG AGCAGTCAAG ACATGGCTCC AGGAACCCCA GGTCCCATCA AGAAGATAGA 
GCCAGTCATG GGCACTCTGC AGAGAGCTCC AGACAATCAG GCACTCGTCA TGCAGAGACT 
TCCTCTGGTG GACAGGCTGC ATCATCCCAG GAACAGGCAA GGTCAAGTCC AGGAGAAAGA 
CATGGATCCC GCCACCAGCA GTCAGCAGAC AGCTCCACAG ACTCAGGCAC TGGGCGCAGA 
CAAGATTCAT CTGTAGTCGG AGACAGTGGA AACCGAGGGT CCAGTGGTAG CCAGGCCAGT 
GACAGCGAGG GACACTCAGA AGAGTCAGAC ACACAGTCAG TGTCAGCCCA CGGACAGGCT 
GGGCCCCATC AGCAGAGCCA CCAAGAGTCC ACACGTGGCC AGTCAGGGGA AAGGTCTGGA 
CGTTCAGGGT CTTTCCTCTA CCAGGTGAGC ACTCATGAAC AGTCTGAGTC CGCCCATGGA 
CGCACAGGGC CCAGCACTGG AGGAAGACAA AGATCCCGCC ACGAGCAGGC ACGAGACAGC 
TCCAGGCACT CAGCGTCCCA AGAGGGTCAG GACACCATTC GTGGACACCC AGGGTCAAGC 
AGAGGAGGAA GGCAGGGATC CCACTATGAG CAATCGGTAG ATAGTTCTGG ACACTCAGGG 
TCTCATCACA GCCACACCAC GTCCCAGGAA AGGTCTGATG TCTCCCGTGG GCAGTCAGGA 
TCCAGAAGTG TCAGCAGACA AACACGTAAT GAGAAACAAT CAGGAGACGG CTCCAGGCAC 
TCAGGGTCGC GTCACCATGA AGCTTCCTCT CGGGCCGACA GCTCTAGACA CTCGCAGGTG 
GGCCAGGGAC AATCATCAGG GCCCAGGACA AGCAGGAACC AGGGATCCAG TGTTAGCCAG 
GACAGTGACA GTCAGGGACA CTCAGAAGAC TCTGAGAGGT GGTCTGGGTC TGCTTCCAGA 
AACCATCTTG GATCTGCTTG GGAGCAGTCA AGAGATGGCT CCAGACACCC TGGGTCCCAT 
CACGAAGACA GAGCCGGTCA CGGGCACTCT GCAGACAGCT CCAGACAATC AGGCACTCGT 
CACACAGAGT CTTCCTCTCG TGGACAGGCT GCGTCATCCC ATGAACAGGC AAGATCAAGT 
GCAGGAGAAA GACATGGATC CCACCACCAG CTCCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ATGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTACAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCACAGGGAA AAGCTGGGCC CCATCAGCAG AGCCACAAAG AGTCCGCACG TGGCCAGTCA 
GGGGAAAGCT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCCACCC ATGGACAGTC TGCGCCCAGC ACTGGAGGAA GACAAGGATC CCATTATGAT 
CAGGCACAAG ACAGCTCCAG GCACTCAGCA TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGC CAAGCAGAGG AGGAAGACAG GGGTCCCACC AAGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCTCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CGTGGGCAGT CAGGATCCAG AAGTGCAAGC AGAAAAACAT ATGACAAGGA ACAATCAGGA 
GATGGCTCTA GGCACTCAGG GTCGCATCAT CATGAAGCTT CCTCTTGGGC CGACAGCTCT 
AGACACTCAC TGGTGGGCCA GGGACAATCA TCAGGGCCCA GGACAAGCAG GCCCCGGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GGACACTCAG AAGATTCTGA GAGGCGGTCT 
GGGTCTGCGT CCAGAAACCA TCATGGATCT GCTCAGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCAGGT CCCATCACGA AGACAGAGCC GGTCATGGGC ACTCTGCAGA GAGCTCCAGA 
CAATCAGGCA CTCATCATGC AGAGAATTCC TCTGGTGGAC AGGCTGCATC ATCCCATGAA 
CAGGCAAGAT CAAGTGCAGG AGAGAGACAC GGATCCCACC ACCAGCAGTC AGCAGACAGC 
TCCAGACACT CAGGCATTGG GCACGGACAA GCTTCATCTG CAGTCAGAGA CAGTGGACAC 
CGAGGGTCCA GTGGTAGTCA GGCCAGTGAC AGTGAGGGAC ATTCAGAAGA CTCAGACACA 
CAGTCAGTGT CAGCCCACGG ACAGGCTGGG CCCCATCAGC AGAGCCACCA AGAGTCCACA 
CGTGGCCGGT CAGCAGGAAG GTCTGGACGT TCAGGGTCTT TCCTCTACCA GGTGAGCACT 
CATGAACAGT CTGAGTCCGC CCATGGACGG ACCGGGACCA GCACTGGAGG AAGACAAGGA 
TCCCACCACA AGCAGGCACG AGACAGCTCC AGGCACTCAA CGTCCCAAGA GGGTCAGGAC 
ACCATTCATG GACACCCGGG GTCAAGCAGT GGAGGAAGGC AGGGATCCCA CTACGAGCAA 
TTGGTAGATA GATCTGGACA CTCAGGGTCT CATCACAGCC ACACCACATC CCAGGGAAGG 
TCTGATGCCT CCCATGGGCA CTCAGGATCC AGAAGTGCAA GCAGACAAAC TCGTAACGAT 
GAACAATCAG GAGACGGCTC CAGGCACTCA GGGTCGCGTC ACCATGAAGC TTCCTCTCGG 
GCCGACAGCT CTGGACACTC GCAGGTGGGC CAGGGACAAT CAGAGGGGCC CAGGACAAGC 
AGGAACTGGG GATCCAGTTT TAGCCAGGAC AGTGACAGTC AGGGACACTC AGAAGACTCT 
GAGAGGTGGT CTGGGTCTGC TTCCAGAAAC CATCATGGAT CTGCTCAGGA GCAGCTAAGA 
GATGGCTCCA GACACCCCAG GTCCCATCAA GAAGACAGAG CTGGTCATGG GCACTCTGCA 
GACAGCTCCA GACAATCAGG CACTCGTCAC ACACAGACTT CCTCTGGTGG ACAGGCTGCA 
TCATCCCATG AACAGGCAAG ATCAAGTGCA GGAGAAAGAC ATGGATCCCA CCACCAGCAG 
TCAGCAGACA GCTCCAGACA CTCAGGCATT GGGCACGGAC AAGCTTCATC TGCAGTCAGA 
GACAGTGGAC ACCGAGGGTA CAGTGGTAGT CAGGCCAGTG ACAATGAGGG ACATTCAGAA 
GACTCAGACA CACAGTCAGT GTCAGCCCAC GGACAGGCTG GGTCCCATCA GCAGAGCCAC 
CAAGAGTCCG CACGTGGCCG GTCAGGGGAA ACGTCTGGAC ATTCAGGATC TTTCCTCTAC 
CAGGTGAGCA CTCATGAACA GTCTGAGTCC TCCCATGGAT GGACGGGGCC CAGCACTAGA 
GGAAGACAAG GATCCCGCCA TGAGCAGGCA CAAGACAGCT CCAGGCACTC AGCATCCCAA 
GACGGTCAGG ACACCATTCG TGGACACCCG GGGTCAAGCA GAGGAGGAAG GCAGGGGTAC 
CACCACGAGC ATTCGGTAGA TAGCTCTGGA CACTCAGGGT CCCATCACAG CCACACCACA 
TCCCAGGGAA GGTCTGATGC CTCCCGTGGG CAGTCAGGAT CCAGAAGTGC AAGCAGAACA 
ACACGTAATG AGGAACAATC AGGAGACGGC TCCAGGCACT CAGGGTCGCG TCACCATGAA 
GCTTCCACTC ATGCCGACAT CTCTAGACAC TCACAGGCAG TCCAGGGACA ATCAGAGGGG 
TCCAGGAGAA GCAGGCGCCA GGGATCCAGT GTGAGCCAGG ACAGTGACAG TGAGGGACAT 
TCAGAAGACT CTGAGAGGTG GTCTGGGTCT GCTTCCAGAA ACCATCATGG ATCTGCTCAG 
GAGCAGCTAA GAGATGGCTC CAGACACCCC AGGTCCCATC AAGAAGACAG AGCTGGTCAT 
GGGCACTCTG CAGACAGCTC CAGACAATCA GGCACTCGTC ACACACAGAC TTCCTCTGGT 
GGACAGGCTG CATCATCCCA TGAACAGGCA AGATCAAGTG CAGGAGAAAG ACATGGATCC 
CACCACCAGC AGTCAGCAGA CAGCTCCAGA CACTCAGGCA TTGGGCACGG ACAAGCTTCA 
TCTGCAGTCA GAGACAGTGG ACACCGAGGG TACAGTGGTA GTCAGGCCAG TGACAATGAG 
GGACATTCAG AAGACTCAGA CACACAGTCA GTGTCAGCCC ACGGACAGGC TGGGTCCCAT 
CAGCAGAGCC ACCAAGAGTC CGCACGTGGC CGGTCAGGGG AAACGTCTGG ACATTCAGGA 
TCTTTCCTCT ACCAGGTGAG CACTCATGAA CAGTCTGAGT CCTCCCATGG ATGGACGGGG 
CCCAGCACTA GAGGAAGACA AGGATCCCGC CATGAGCAGG CACAAGACAG CTCCAGGCAC 
TCAGCATCCC AATACGGTCA GGACACCATT CGTGGACACC CGGGGTCAAG CAGAGGAGGA 
AGGCAGGGGT ACCACCACGA GCATTCGGTA GATAGCTCTG GACACTCAGG GTCCCATCAC 
AGCCACACCA CATCCCAGGG AAGGTCTGAT GCCTCCCGTG GGCAGTCAGG ATCCAGAAGT 
GCAAGCAGAA CAACACGTAA TGAGGAACAA TCAGGAGACA GCTCCAGGCA CTCAGTGTCA 
CGTCACCATG AAGCTTCCAC TCATGCCGAC ATCTCTAGAC ACTCACAGGC AGTCCAGGGA 
CAATCAGAGG GGTCCAGGAG AAGCAGGCGC CAGGGATCCA GTGTGAGCCA GGACAGTGAC 
AGTGAGGGAC ATTCAGAAGA CTCTGAGAGG TGGTCTGGGT CTGCTTCCAG AAACCATCGT 
GGATCTGTTC AGGAGCAGTC AAGGCACGGC TCCAGACACC CCAGGTCCCA TCACGAAGAC 
AGAGCCGGTC ACGGGCACTC TGCAGACCGC TCCAGACAAT CAGGCACTCG TCACGCAGAG 
ACTTCCTCTG GTGGACAGGC TGCATCATCC CATGAACAGG CAAGATCAAG TCCAGGAGAG 
AGACACGGAT CCCGCCACCA GCAGTCAGCA GACAGCTCCA GACACTCAGG CATTCCGCGT 
GGACAAGCTT CATCTGCAGT CAGAGACAGT AGACACTGGG GGTCCAGTGG TAGTCAGGCC 
AGTGATAGTG AGGGACATTC AGAAGAGTCA GACACACAGT CAGTGTCAGG CCATGGACAG 
GCTGGGCCCC ATCAGCAGAG CCACCAAGAG TCCGCACGTG ACCGGTCAGG GGGAAGGTCT 
GGACGTTCAG GGTCTTTCCT CTACCAGGTG AGCACTCATG AACAGTCTGA GTCTGCCCAT 
GGGCGGACCA GGACCAGCAC TGGACGAAGA CAAGGATCCC ACCACGAGCA GGCACGAGAC 
AGCTCCAGGC ACTCAGCGTC CCAAGAGGGT CAGGACACCA TTCGTGGACA CCCGGGGTCA 
AGCAGAAGAG GAAGGCAGGG ATCCCACTAC GAGCAATCGG TAGATAGGTC TGGACACTCA 
GGGTCCCATC ACAGCCACAC CACATCCCAG GGAAGGTCTG ATGCCTCCCG TGGGCAGTCA 
GGATCCAGAA GTGCCAGCAG ACAAACTCGT AATGACGAAC AATCAGGAGA TGGCTCCAGG 
CACTCATGGT CGCATCACCA TGAAGCTTCC ACTCAGGCGG ACAGCTCTAG ACACTCACAG 
TCCGGCCAGG GACAATCAGC GGGGCCCAGG ACAAGCAGGA ACCAGGGATC CAGTGTTAGC 
CAGGACAGTG ACAGTCAGGG ACACTCAGAA GACTCTGAGA GGTGGTCTGG GTCTGCTTCC 
AGAAACCATC GTGGATCTGC TCAGGAGCAG TCAAGAGATG GCTCCAGACA CCCCACGTCC 
CATCACGAAG ACAGAGCCGG TCACGGGCAC TCTGCAGAGA GCTCCAGACA ATCAGGCACT 
CATCATGCAG AGAATTCCTC TGGTGGACAG GCTGCATCAT CCCATGAACA GGCAAGATCA 
AGTGCAGGAG AGAGACATGG ATCCCACCAC CAGCAGTCAG CAGACAGCTC CAGACACTCA 
GGCATTGGGC ACGGACAAGC TTCATCTGCA GTCAGAGACA GTGGACACCG AGGGTCCAGT 
GGTAGTCAGG CCAGTGACAG TGAGGGACAT TCAGAAGACT CAGACACACA GTCAGTGTCA 
GCCCACGGAC AGGCTGGGCC CCATCAGCAG AGCCACCAAG AGTCCACACG TGGCCGGTCA 
GCAGGAAGGT CTGGACGTTC AGGGTCTTTC CTCTACCAGG TGAGCACTCA TGAACAGTCT 
GAGTCTGCCC ATGGACGGGC TGGGCCCAGT ACTGGAGGAA GACAAGGATC CCGCCACGAG 
CAGGCACGAG ACAGCTCCAG GCACTCAGCG TCCCAAGAGG GTCAGGACAC CATTCGTGGA 
CACCCGGGGT CAAGGAGAGG AGGAAGACAG GGATCCTACC ACGAGCAATC GGTAGATAGG 
TCTGGACACT CAGGGTCCCA TCACAGCCAC ACCACATCCC AGGGAAGGTC TGATGCCTCC 
CATGGGCAGT CAGGATCCAG AAGTGCAAGC AGAGAAACAC GTAATGAGGA ACAGTCAGGA 
GACGGCTCCA GGCACTCAGG GTCGCGTCAC CATGAAGCTT CCACTCAGGC TGACAGCTCT 
AGACACTCAC AGTCCGGCCA GGGTGAATCA GCGGGGTCCA GGAGAAGCAG GCGCCAGGGA 
TCCAGTGTTA GCCAGGACAG TGACAGTGAG GCATACCCAG AGGACTCTGA GAGGCGATCT 
GAGTCTGCTT CCAGAAACCA TCATGGATCT TCTCGGGAGC AGTCAAGAGA TGGCTCCAGA 
CACCCCGGAT CCTCTCACCG CGATACAGCC AGTCATGTAC AGTCTTCACC TGTACAGTCA 
GACTCTAGTA CCGCTAAGGA ACATGGTCAC TTTAGTAGTC TTTCACAAGA TTCTGCGTAT 
CACTCAGGAA TACAGTCACG TGGCAGTCCT CACAGTTCTA GTTCTTATCA TTATCAATCT 
GAGGGCACTG AAAGGCAAAA AGGTCAATCA GGTTTAGTTT GGAGACATGG CAGCTATGGT 
AGTGCAGATT ATGATTATGG TGAATCCGGG TTTAGACACT CTCAGCACGG AAGTGTTAGT 
TACAATTCCA ATCCTGTTGT TTTCAAGGAA AGATCTGATA TCTGTAAAGC AAGTGCGTTT 
GGTAAAGATC ATCCAAGGTA TTATGCAACG TATATTAATA AGGACCCAGG TTTATGTGGC 
CATTCTAGTG ATATATCGAA ACAACTGGGA TTTAGTCAGT CACAGAGATA CTATTACTAT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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FLG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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