MRPS27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPS27
Entrez Gene ID	23107
Full Name	mitochondrial ribosomal protein S27
Synonyms	MRP-S27,S27mt
General protein information	Preferred Names mitochondrial ribosomal protein S27 Names 28S ribosomal protein S27, mitochondrial mitochondrial 28S ribosomal protein S27 mitochondrial small ribosomal subunit protein mS27
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.2
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013].
Disorder MIM:	611989

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286748.1	NP_001273677.1	28S ribosomal protein S27, mitochondrial isoform 1
NM_015084.2	NP_055899.2	28S ribosomal protein S27, mitochondrial isoform 2
NM_001286748.2	NP_001273677.1	28S ribosomal protein S27, mitochondrial isoform 1
NM_015084.3	NP_055899.2	28S ribosomal protein S27, mitochondrial isoform 2
NM_001286751.1	NP_001273680.1	28S ribosomal protein S27, mitochondrial isoform 3
NM_001286751.2	NP_001273680.1	28S ribosomal protein S27, mitochondrial isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MRPS27	XP_517715.2
Rattus norvegicus (Norway rat)	Mrps27	NP_001186121.1
Danio rerio (zebrafish)	mrps27	NP_001003778.1
	MRPS27	XP_001098829.1
Mus musculus (house mouse)	Mrps27	NP_776118.2
Canis lupus familiaris (dog)	MRPS27	XP_852973.1
Bos taurus (cattle)	MRPS27	NP_001033197.1
Gallus gallus (chicken)	MRPS27	XP_424803.1
Xenopus tropicalis (tropical clawed frog)	mrps27	XP_004910871.1
Homo sapiens (human)	MRPS27	NP_055899.2

Gene Ontology
Bibliography

Related articles in PubMed

High MRPS23 expression contributes to hepatocellular carcinoma proliferation and indicates poor survival outcomes.
Pu M, Wang J, Huang Q, Zhao G, Xia C, Shang R, Zhang Z, Bian Z, Yang X, Tao K
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(7)1010428317709127(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.
Davies SM, Lopez Sanchez MI, Narsai R, Shearwood AM, Razif MF, Small ID, Whelan J, Rackham O, Filipovska A
FEBS letters586(20)3555-61(2012 Oct)

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.
Surovtseva YV, Shutt TE, Cotney J, Cimen H, Chen SY, Koc EC, Shadel GS
Proceedings of the National Academy of Sciences of the United States of America108(44)17921-6(2011 Nov)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we verified for the first time that mitochondrial ribosomal protein S23 expression was upregulated in hepatocellular carcinoma. High mitochondrial ribosomal protein S23 levels can predict poor clinical outcomes in hepatocellular carcinoma, and this protein plays a key role in tumor proliferation. Therefore, mitochondrial ribosomal protein S23 may be a potential therapeutic target for hepatocellular carcinoma.
Title: High MRPS23 expression contributes to hepatocellular carcinoma proliferation and indicates poor survival outcomes.

MRPS27 is required for mitochondrial protein synthesis
Title: MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPS27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09212	NM_015084.3 Latest version!	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$174.50-$244.30 $349.00
OHu12397	NM_001286751.2 Latest version!	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu00845	NM_001286748.2 Latest version!	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu00845	NM_001286748.1	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09212	NM_015084.2	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$174.50-$244.30 $349.00
OHu12397	NM_001286751.1	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPS27 ( NM_015084.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055899.2 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09212
Clone ID Related Accession (Same CDS sequence)	NM_015084.2 , NM_015084.3
Accession Version	NM_015084.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC341760.1, AK025417.1 and BM995930.1. On Apr 29, 2008 this sequence version replaced NM_015084.1. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.118629.1, SRR1803613.155316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AGTTTCGACA CAGCCCCAAC 
TGCTGGTACC TGAGAAACTG GACTATCCAC ACCTGGATTA GGCAGTGTCT AAAATATGAT 
GCACAAGACA AAGCCCTATA TACCCTTGTA AATAAGGTTC AATATGGAAT TTTTCCAGAT 
AACTTTACAT TCAATTTACT GATGGATTCT TTCATAAAGA AAGAAAATTA CAAAGATGCT 
TTATCTGTGG TTTTTGAGGT CATGATGCAA GAAGCCTTTG AAGTGCCTTC CACCCAACTT 
CTCTCCCTCT ATGTTTTATT TCATTGCCTG GCAAAGAAGA CAGACTTCAG TTGGGAAGAG 
GAGAGGAACT TTGGTGCATC CCTTTTGCTT CCAGGCCTAA AACAAAAGAA CTCAGTGGGT 
TTCAGTTCCC AGTTGTATGG CTATGCACTT CTTGGGAAGG TGGAGTTGCA GCAAGGGCTA 
CGGGCTGTGT ACCACAACAT GCCTCTGATA TGGAAACCAG GCTACCTTGA CAGAGCCCTT 
CAAGTGATGG AGAAAGTGGC TGCCTCCCCA GAAGACATAA AGCTGTGTAG AGAAGCGCTC 
GATGTGCTGG GTGCAGTGCT GAAGGCTCTG ACTTCAGCTG ATGGGGCTTC AGAGGAGCAG 
TCCCAAAATG ATGAAGACAA CCAGGGGTCA GAAAAACTGG TGGAGCAGTT AGACATCGAG 
GAAACAGAGC AGTCCAAGCT TCCTCAATAC CTGGAACGAT TTAAGGCCTT ACATTCTAAG 
CTTCAAGCTC TGGGCAAAAT TGAGTCAGAA GGTCTTTTAA GTCTGACCAC CCAGCTTGTC 
AAGGAAAAAC TCTCCACCTG TGAAGCAGAG GACATCGCCA CCTATGAGCA GAATCTGCAG 
CAGTGGCATC TAGACCTTGT ACAGTTGATC CAGAGAGAAC AGCAACAGAG GGAGCAAGCG 
AAGCAGGAGT ACCAGGCTCA GAAAGCAGCA AAGGCATCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055899.2
CDS	37..1281
Translation	MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_015084.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015084.2

ORF Insert Sequence:

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AGTTTCGACA CAGCCCCAAC 
TGCTGGTACC TGAGAAACTG GACTATCCAC ACCTGGATTA GGCAGTGTCT AAAATATGAT 
GCACAAGACA AAGCCCTATA TACCCTTGTA AATAAGGTTC AATATGGAAT TTTTCCAGAT 
AACTTTACAT TCAATTTACT GATGGATTCT TTCATAAAGA AAGAAAATTA CAAAGATGCT 
TTATCTGTGG TTTTTGAGGT CATGATGCAA GAAGCCTTTG AAGTGCCTTC CACCCAACTT 
CTCTCCCTCT ATGTTTTATT TCATTGCCTG GCAAAGAAGA CAGACTTCAG TTGGGAAGAG 
GAGAGGAACT TTGGTGCATC CCTTTTGCTT CCAGGCCTAA AACAAAAGAA CTCAGTGGGT 
TTCAGTTCCC AGTTGTATGG CTATGCACTT CTTGGGAAGG TGGAGTTGCA GCAAGGGCTA 
CGGGCTGTGT ACCACAACAT GCCTCTGATA TGGAAACCAG GCTACCTTGA CAGAGCCCTT 
CAAGTGATGG AGAAAGTGGC TGCCTCCCCA GAAGACATAA AGCTGTGTAG AGAAGCGCTC 
GATGTGCTGG GTGCAGTGCT GAAGGCTCTG ACTTCAGCTG ATGGGGCTTC AGAGGAGCAG 
TCCCAAAATG ATGAAGACAA CCAGGGGTCA GAAAAACTGG TGGAGCAGTT AGACATCGAG 
GAAACAGAGC AGTCCAAGCT TCCTCAATAC CTGGAACGAT TTAAGGCCTT ACATTCTAAG 
CTTCAAGCTC TGGGCAAAAT TGAGTCAGAA GGTCTTTTAA GTCTGACCAC CCAGCTTGTC 
AAGGAAAAAC TCTCCACCTG TGAAGCAGAG GACATCGCCA CCTATGAGCA GAATCTGCAG 
CAGTGGCATC TAGACCTTGT ACAGTTGATC CAGAGAGAAC AGCAACAGAG GGAGCAAGCG 
AAGCAGGAGT ACCAGGCTCA GAAAGCAGCA AAGGCATCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS27 ( NM_015084.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055899.2 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09212
Clone ID Related Accession (Same CDS sequence)	NM_015084.2 , NM_015084.3
Accession Version	NM_015084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC341760.1, AK025417.1, BM995930.1 and AC012609.7. On Jun 2, 2019 this sequence version replaced NM_015084.2. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.64992.1, SRR1660807.230461.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AGTTTCGACA CAGCCCCAAC 
TGCTGGTACC TGAGAAACTG GACTATCCAC ACCTGGATTA GGCAGTGTCT AAAATATGAT 
GCACAAGACA AAGCCCTATA TACCCTTGTA AATAAGGTTC AATATGGAAT TTTTCCAGAT 
AACTTTACAT TCAATTTACT GATGGATTCT TTCATAAAGA AAGAAAATTA CAAAGATGCT 
TTATCTGTGG TTTTTGAGGT CATGATGCAA GAAGCCTTTG AAGTGCCTTC CACCCAACTT 
CTCTCCCTCT ATGTTTTATT TCATTGCCTG GCAAAGAAGA CAGACTTCAG TTGGGAAGAG 
GAGAGGAACT TTGGTGCATC CCTTTTGCTT CCAGGCCTAA AACAAAAGAA CTCAGTGGGT 
TTCAGTTCCC AGTTGTATGG CTATGCACTT CTTGGGAAGG TGGAGTTGCA GCAAGGGCTA 
CGGGCTGTGT ACCACAACAT GCCTCTGATA TGGAAACCAG GCTACCTTGA CAGAGCCCTT 
CAAGTGATGG AGAAAGTGGC TGCCTCCCCA GAAGACATAA AGCTGTGTAG AGAAGCGCTC 
GATGTGCTGG GTGCAGTGCT GAAGGCTCTG ACTTCAGCTG ATGGGGCTTC AGAGGAGCAG 
TCCCAAAATG ATGAAGACAA CCAGGGGTCA GAAAAACTGG TGGAGCAGTT AGACATCGAG 
GAAACAGAGC AGTCCAAGCT TCCTCAATAC CTGGAACGAT TTAAGGCCTT ACATTCTAAG 
CTTCAAGCTC TGGGCAAAAT TGAGTCAGAA GGTCTTTTAA GTCTGACCAC CCAGCTTGTC 
AAGGAAAAAC TCTCCACCTG TGAAGCAGAG GACATCGCCA CCTATGAGCA GAATCTGCAG 
CAGTGGCATC TAGACCTTGT ACAGTTGATC CAGAGAGAAC AGCAACAGAG GGAGCAAGCG 
AAGCAGGAGT ACCAGGCTCA GAAAGCAGCA AAGGCATCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055899.2
CDS	20..1264
Translation	MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_015084.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015084.3

ORF Insert Sequence:

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AGTTTCGACA CAGCCCCAAC 
TGCTGGTACC TGAGAAACTG GACTATCCAC ACCTGGATTA GGCAGTGTCT AAAATATGAT 
GCACAAGACA AAGCCCTATA TACCCTTGTA AATAAGGTTC AATATGGAAT TTTTCCAGAT 
AACTTTACAT TCAATTTACT GATGGATTCT TTCATAAAGA AAGAAAATTA CAAAGATGCT 
TTATCTGTGG TTTTTGAGGT CATGATGCAA GAAGCCTTTG AAGTGCCTTC CACCCAACTT 
CTCTCCCTCT ATGTTTTATT TCATTGCCTG GCAAAGAAGA CAGACTTCAG TTGGGAAGAG 
GAGAGGAACT TTGGTGCATC CCTTTTGCTT CCAGGCCTAA AACAAAAGAA CTCAGTGGGT 
TTCAGTTCCC AGTTGTATGG CTATGCACTT CTTGGGAAGG TGGAGTTGCA GCAAGGGCTA 
CGGGCTGTGT ACCACAACAT GCCTCTGATA TGGAAACCAG GCTACCTTGA CAGAGCCCTT 
CAAGTGATGG AGAAAGTGGC TGCCTCCCCA GAAGACATAA AGCTGTGTAG AGAAGCGCTC 
GATGTGCTGG GTGCAGTGCT GAAGGCTCTG ACTTCAGCTG ATGGGGCTTC AGAGGAGCAG 
TCCCAAAATG ATGAAGACAA CCAGGGGTCA GAAAAACTGG TGGAGCAGTT AGACATCGAG 
GAAACAGAGC AGTCCAAGCT TCCTCAATAC CTGGAACGAT TTAAGGCCTT ACATTCTAAG 
CTTCAAGCTC TGGGCAAAAT TGAGTCAGAA GGTCTTTTAA GTCTGACCAC CCAGCTTGTC 
AAGGAAAAAC TCTCCACCTG TGAAGCAGAG GACATCGCCA CCTATGAGCA GAATCTGCAG 
CAGTGGCATC TAGACCTTGT ACAGTTGATC CAGAGAGAAC AGCAACAGAG GGAGCAAGCG 
AAGCAGGAGT ACCAGGCTCA GAAAGCAGCA AAGGCATCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS27 ( NM_001286751.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273680.1 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12397
Clone ID Related Accession (Same CDS sequence)	NM_001286751.1 , NM_001286751.2
Accession Version	NM_001286751.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026406.5, BC011818.1, AC012609.7 and BM995930.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC011818.1, SRR1660805.233858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGATAAAA CATTTGAGAG AAAGTTGCCT GTTAGTTCTT TAACAATATC ACGGCTTATA 
GACAACATTT CCTCTCGGGA AGAGATAGAT CATGCAGAGT ATTACCTTTA CAAGTTTCGA 
CACAGCCCCA ACTGCTGGTA CCTGAGAAAC TGGACTATCC ACACCTGGAT TAGGCAGTGT 
CTAAAATATG ATGCACAAGA CAAAGCCCTA TATACCCTTG TAAATAAGGT TCAATATGGA 
ATTTTTCCAG ATAACTTTAC ATTCAATTTA CTGATGGATT CTTTCATAAA GAAAGAAAAT 
TACAAAGATG CTTTATCTGT GGTTTTTGAG GTCATGATGC AAGAAGCCTT TGAAGTGCCT 
TCCACCCAAC TTCTCTCCCT CTATGTTTTA TTTCATTGCC TGGCAAAGAA GACAGACTTC 
AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA TCCCTTTTGC TTCCAGGCCT AAAACAAAAG 
AACTCAGTGG GTTTCAGTTC CCAGTTGTAT GGCTATGCAC TTCTTGGGAA GGTGGAGTTG 
CAGCAAGGGC TACGGGCTGT GTACCACAAC ATGCCTCTGA TATGGAAACC AGGCTACCTT 
GACAGAGCCC TTCAAGTGAT GGAGAAAGTG GCTGCCTCCC CAGAAGACAT AAAGCTGTGT 
AGAGAAGCGC TCGATGTGCT GGGTGCAGTG CTGAAGGCTC TGACTTCAGC TGATGGGGCT 
TCAGAGGAGC AGTCCCAAAA TGATGAAGAC AACCAGGGGT CAGAAAAACT GGTGGAGCAG 
TTAGACATCG AGGAAACAGA GCAGTCCAAG CTTCCTCAAT ACCTGGAACG ATTTAAGGCC 
TTACATTCTA AGCTTCAAGC TCTGGGCAAA ATTGAGTCAG AAGGTCTTTT AAGTCTGACC 
ACCCAGCTTG TCAAGGAAAA ACTCTCCACC TGTGAAGCAG AGGACATCGC CACCTATGAG 
CAGAATCTGC AGCAGTGGCA TCTAGACCTT GTACAGTTGA TCCAGAGAGA ACAGCAACAG 
AGGGAGCAAG CGAAGCAGGA GTACCAGGCT CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273680.1
CDS	173..1249
Translation	MDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_001286751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286751.1

ORF Insert Sequence:

ATGGATAAAA CATTTGAGAG AAAGTTGCCT GTTAGTTCTT TAACAATATC ACGGCTTATA 
GACAACATTT CCTCTCGGGA AGAGATAGAT CATGCAGAGT ATTACCTTTA CAAGTTTCGA 
CACAGCCCCA ACTGCTGGTA CCTGAGAAAC TGGACTATCC ACACCTGGAT TAGGCAGTGT 
CTAAAATATG ATGCACAAGA CAAAGCCCTA TATACCCTTG TAAATAAGGT TCAATATGGA 
ATTTTTCCAG ATAACTTTAC ATTCAATTTA CTGATGGATT CTTTCATAAA GAAAGAAAAT 
TACAAAGATG CTTTATCTGT GGTTTTTGAG GTCATGATGC AAGAAGCCTT TGAAGTGCCT 
TCCACCCAAC TTCTCTCCCT CTATGTTTTA TTTCATTGCC TGGCAAAGAA GACAGACTTC 
AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA TCCCTTTTGC TTCCAGGCCT AAAACAAAAG 
AACTCAGTGG GTTTCAGTTC CCAGTTGTAT GGCTATGCAC TTCTTGGGAA GGTGGAGTTG 
CAGCAAGGGC TACGGGCTGT GTACCACAAC ATGCCTCTGA TATGGAAACC AGGCTACCTT 
GACAGAGCCC TTCAAGTGAT GGAGAAAGTG GCTGCCTCCC CAGAAGACAT AAAGCTGTGT 
AGAGAAGCGC TCGATGTGCT GGGTGCAGTG CTGAAGGCTC TGACTTCAGC TGATGGGGCT 
TCAGAGGAGC AGTCCCAAAA TGATGAAGAC AACCAGGGGT CAGAAAAACT GGTGGAGCAG 
TTAGACATCG AGGAAACAGA GCAGTCCAAG CTTCCTCAAT ACCTGGAACG ATTTAAGGCC 
TTACATTCTA AGCTTCAAGC TCTGGGCAAA ATTGAGTCAG AAGGTCTTTT AAGTCTGACC 
ACCCAGCTTG TCAAGGAAAA ACTCTCCACC TGTGAAGCAG AGGACATCGC CACCTATGAG 
CAGAATCTGC AGCAGTGGCA TCTAGACCTT GTACAGTTGA TCCAGAGAGA ACAGCAACAG 
AGGGAGCAAG CGAAGCAGGA GTACCAGGCT CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS27 ( NM_001286751.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273680.1 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12397
Clone ID Related Accession (Same CDS sequence)	NM_001286751.1 , NM_001286751.2
Accession Version	NM_001286751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026406.5, BC011818.1, AC012609.7 and BM995930.1. On Jun 2, 2019 this sequence version replaced NM_001286751.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.121891.1, BC011818.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGATAAAA CATTTGAGAG AAAGTTGCCT GTTAGTTCTT TAACAATATC ACGGCTTATA 
GACAACATTT CCTCTCGGGA AGAGATAGAT CATGCAGAGT ATTACCTTTA CAAGTTTCGA 
CACAGCCCCA ACTGCTGGTA CCTGAGAAAC TGGACTATCC ACACCTGGAT TAGGCAGTGT 
CTAAAATATG ATGCACAAGA CAAAGCCCTA TATACCCTTG TAAATAAGGT TCAATATGGA 
ATTTTTCCAG ATAACTTTAC ATTCAATTTA CTGATGGATT CTTTCATAAA GAAAGAAAAT 
TACAAAGATG CTTTATCTGT GGTTTTTGAG GTCATGATGC AAGAAGCCTT TGAAGTGCCT 
TCCACCCAAC TTCTCTCCCT CTATGTTTTA TTTCATTGCC TGGCAAAGAA GACAGACTTC 
AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA TCCCTTTTGC TTCCAGGCCT AAAACAAAAG 
AACTCAGTGG GTTTCAGTTC CCAGTTGTAT GGCTATGCAC TTCTTGGGAA GGTGGAGTTG 
CAGCAAGGGC TACGGGCTGT GTACCACAAC ATGCCTCTGA TATGGAAACC AGGCTACCTT 
GACAGAGCCC TTCAAGTGAT GGAGAAAGTG GCTGCCTCCC CAGAAGACAT AAAGCTGTGT 
AGAGAAGCGC TCGATGTGCT GGGTGCAGTG CTGAAGGCTC TGACTTCAGC TGATGGGGCT 
TCAGAGGAGC AGTCCCAAAA TGATGAAGAC AACCAGGGGT CAGAAAAACT GGTGGAGCAG 
TTAGACATCG AGGAAACAGA GCAGTCCAAG CTTCCTCAAT ACCTGGAACG ATTTAAGGCC 
TTACATTCTA AGCTTCAAGC TCTGGGCAAA ATTGAGTCAG AAGGTCTTTT AAGTCTGACC 
ACCCAGCTTG TCAAGGAAAA ACTCTCCACC TGTGAAGCAG AGGACATCGC CACCTATGAG 
CAGAATCTGC AGCAGTGGCA TCTAGACCTT GTACAGTTGA TCCAGAGAGA ACAGCAACAG 
AGGGAGCAAG CGAAGCAGGA GTACCAGGCT CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273680.1
CDS	156..1232
Translation	MDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_001286751.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286751.2

ORF Insert Sequence:

ATGGATAAAA CATTTGAGAG AAAGTTGCCT GTTAGTTCTT TAACAATATC ACGGCTTATA 
GACAACATTT CCTCTCGGGA AGAGATAGAT CATGCAGAGT ATTACCTTTA CAAGTTTCGA 
CACAGCCCCA ACTGCTGGTA CCTGAGAAAC TGGACTATCC ACACCTGGAT TAGGCAGTGT 
CTAAAATATG ATGCACAAGA CAAAGCCCTA TATACCCTTG TAAATAAGGT TCAATATGGA 
ATTTTTCCAG ATAACTTTAC ATTCAATTTA CTGATGGATT CTTTCATAAA GAAAGAAAAT 
TACAAAGATG CTTTATCTGT GGTTTTTGAG GTCATGATGC AAGAAGCCTT TGAAGTGCCT 
TCCACCCAAC TTCTCTCCCT CTATGTTTTA TTTCATTGCC TGGCAAAGAA GACAGACTTC 
AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA TCCCTTTTGC TTCCAGGCCT AAAACAAAAG 
AACTCAGTGG GTTTCAGTTC CCAGTTGTAT GGCTATGCAC TTCTTGGGAA GGTGGAGTTG 
CAGCAAGGGC TACGGGCTGT GTACCACAAC ATGCCTCTGA TATGGAAACC AGGCTACCTT 
GACAGAGCCC TTCAAGTGAT GGAGAAAGTG GCTGCCTCCC CAGAAGACAT AAAGCTGTGT 
AGAGAAGCGC TCGATGTGCT GGGTGCAGTG CTGAAGGCTC TGACTTCAGC TGATGGGGCT 
TCAGAGGAGC AGTCCCAAAA TGATGAAGAC AACCAGGGGT CAGAAAAACT GGTGGAGCAG 
TTAGACATCG AGGAAACAGA GCAGTCCAAG CTTCCTCAAT ACCTGGAACG ATTTAAGGCC 
TTACATTCTA AGCTTCAAGC TCTGGGCAAA ATTGAGTCAG AAGGTCTTTT AAGTCTGACC 
ACCCAGCTTG TCAAGGAAAA ACTCTCCACC TGTGAAGCAG AGGACATCGC CACCTATGAG 
CAGAATCTGC AGCAGTGGCA TCTAGACCTT GTACAGTTGA TCCAGAGAGA ACAGCAACAG 
AGGGAGCAAG CGAAGCAGGA GTACCAGGCT CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS27 ( NM_001286748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273677.1 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00845
Clone ID Related Accession (Same CDS sequence)	NM_001286748.1 , NM_001286748.2
Accession Version	NM_001286748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026406.5, AK299412.1, BC003632.2 and BM995930.1. On Nov 15, 2013 this sequence version replaced XM_005248471.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.66191.1, SRR1803616.164864.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AAAAGGGAGA ATGCTCATCC 
AGCAATCCAC AGAATTATTC CAGGTTTCGA CACAGCCCCA ACTGCTGGTA CCTGAGAAAC 
TGGACTATCC ACACCTGGAT TAGGCAGTGT CTAAAATATG ATGCACAAGA CAAAGCCCTA 
TATACCCTTG TAAATAAGGT TCAATATGGA ATTTTTCCAG ATAACTTTAC ATTCAATTTA 
CTGATGGATT CTTTCATAAA GAAAGAAAAT TACAAAGATG CTTTATCTGT GGTTTTTGAG 
GTCATGATGC AAGAAGCCTT TGAAGTGCCT TCCACCCAAC TTCTCTCCCT CTATGTTTTA 
TTTCATTGCC TGGCAAAGAA GACAGACTTC AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA 
TCCCTTTTGC TTCCAGGCCT AAAACAAAAG AACTCAGTGG GTTTCAGTTC CCAGTTGTAT 
GGCTATGCAC TTCTTGGGAA GGTGGAGTTG CAGCAAGGGC TACGGGCTGT GTACCACAAC 
ATGCCTCTGA TATGGAAACC AGGCTACCTT GACAGAGCCC TTCAAGTGAT GGAGAAAGTG 
GCTGCCTCCC CAGAAGACAT AAAGCTGTGT AGAGAAGCGC TCGATGTGCT GGGTGCAGTG 
CTGAAGGCTC TGACTTCAGC TGATGGGGCT TCAGAGGAGC AGTCCCAAAA TGATGAAGAC 
AACCAGGGGT CAGAAAAACT GGTGGAGCAG TTAGACATCG AGGAAACAGA GCAGTCCAAG 
CTTCCTCAAT ACCTGGAACG ATTTAAGGCC TTACATTCTA AGCTTCAAGC TCTGGGCAAA 
ATTGAGTCAG AAGGTCTTTT AAGTCTGACC ACCCAGCTTG TCAAGGAAAA ACTCTCCACC 
TGTGAAGCAG AGGACATCGC CACCTATGAG CAGAATCTGC AGCAGTGGCA TCTAGACCTT 
GTACAGTTGA TCCAGAGAGA ACAGCAACAG AGGGAGCAAG CGAAGCAGGA GTACCAGGCT 
CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273677.1
CDS	37..1323
Translation	MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKKGECSSSNPQNYSRFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_001286748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286748.1

ORF Insert Sequence:

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AAAAGGGAGA ATGCTCATCC 
AGCAATCCAC AGAATTATTC CAGGTTTCGA CACAGCCCCA ACTGCTGGTA CCTGAGAAAC 
TGGACTATCC ACACCTGGAT TAGGCAGTGT CTAAAATATG ATGCACAAGA CAAAGCCCTA 
TATACCCTTG TAAATAAGGT TCAATATGGA ATTTTTCCAG ATAACTTTAC ATTCAATTTA 
CTGATGGATT CTTTCATAAA GAAAGAAAAT TACAAAGATG CTTTATCTGT GGTTTTTGAG 
GTCATGATGC AAGAAGCCTT TGAAGTGCCT TCCACCCAAC TTCTCTCCCT CTATGTTTTA 
TTTCATTGCC TGGCAAAGAA GACAGACTTC AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA 
TCCCTTTTGC TTCCAGGCCT AAAACAAAAG AACTCAGTGG GTTTCAGTTC CCAGTTGTAT 
GGCTATGCAC TTCTTGGGAA GGTGGAGTTG CAGCAAGGGC TACGGGCTGT GTACCACAAC 
ATGCCTCTGA TATGGAAACC AGGCTACCTT GACAGAGCCC TTCAAGTGAT GGAGAAAGTG 
GCTGCCTCCC CAGAAGACAT AAAGCTGTGT AGAGAAGCGC TCGATGTGCT GGGTGCAGTG 
CTGAAGGCTC TGACTTCAGC TGATGGGGCT TCAGAGGAGC AGTCCCAAAA TGATGAAGAC 
AACCAGGGGT CAGAAAAACT GGTGGAGCAG TTAGACATCG AGGAAACAGA GCAGTCCAAG 
CTTCCTCAAT ACCTGGAACG ATTTAAGGCC TTACATTCTA AGCTTCAAGC TCTGGGCAAA 
ATTGAGTCAG AAGGTCTTTT AAGTCTGACC ACCCAGCTTG TCAAGGAAAA ACTCTCCACC 
TGTGAAGCAG AGGACATCGC CACCTATGAG CAGAATCTGC AGCAGTGGCA TCTAGACCTT 
GTACAGTTGA TCCAGAGAGA ACAGCAACAG AGGGAGCAAG CGAAGCAGGA GTACCAGGCT 
CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS27 ( NM_001286748.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273677.1 Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00845
Clone ID Related Accession (Same CDS sequence)	NM_001286748.1 , NM_001286748.2
Accession Version	NM_001286748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	28S ribosomal protein S27, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026406.5, AK299412.1, BC003632.2, BM995930.1 and AC012609.7. On Jun 2, 2019 this sequence version replaced NM_001286748.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.66191.1, SRR1803613.30492.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AAAAGGGAGA ATGCTCATCC 
AGCAATCCAC AGAATTATTC CAGGTTTCGA CACAGCCCCA ACTGCTGGTA CCTGAGAAAC 
TGGACTATCC ACACCTGGAT TAGGCAGTGT CTAAAATATG ATGCACAAGA CAAAGCCCTA 
TATACCCTTG TAAATAAGGT TCAATATGGA ATTTTTCCAG ATAACTTTAC ATTCAATTTA 
CTGATGGATT CTTTCATAAA GAAAGAAAAT TACAAAGATG CTTTATCTGT GGTTTTTGAG 
GTCATGATGC AAGAAGCCTT TGAAGTGCCT TCCACCCAAC TTCTCTCCCT CTATGTTTTA 
TTTCATTGCC TGGCAAAGAA GACAGACTTC AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA 
TCCCTTTTGC TTCCAGGCCT AAAACAAAAG AACTCAGTGG GTTTCAGTTC CCAGTTGTAT 
GGCTATGCAC TTCTTGGGAA GGTGGAGTTG CAGCAAGGGC TACGGGCTGT GTACCACAAC 
ATGCCTCTGA TATGGAAACC AGGCTACCTT GACAGAGCCC TTCAAGTGAT GGAGAAAGTG 
GCTGCCTCCC CAGAAGACAT AAAGCTGTGT AGAGAAGCGC TCGATGTGCT GGGTGCAGTG 
CTGAAGGCTC TGACTTCAGC TGATGGGGCT TCAGAGGAGC AGTCCCAAAA TGATGAAGAC 
AACCAGGGGT CAGAAAAACT GGTGGAGCAG TTAGACATCG AGGAAACAGA GCAGTCCAAG 
CTTCCTCAAT ACCTGGAACG ATTTAAGGCC TTACATTCTA AGCTTCAAGC TCTGGGCAAA 
ATTGAGTCAG AAGGTCTTTT AAGTCTGACC ACCCAGCTTG TCAAGGAAAA ACTCTCCACC 
TGTGAAGCAG AGGACATCGC CACCTATGAG CAGAATCTGC AGCAGTGGCA TCTAGACCTT 
GTACAGTTGA TCCAGAGAGA ACAGCAACAG AGGGAGCAAG CGAAGCAGGA GTACCAGGCT 
CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273677.1
CDS	20..1306
Translation	MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKKGECSSSNPQNYSRFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Target ORF information:

RefSeq Version	NM_001286748.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286748.2

ORF Insert Sequence:

ATGGCTGCCT CCATAGTGCG GCGCGGGATG CTCCTGGCGC GGCAAGTGGT TCTTCCTCAG 
CTCTCTCCTG CAGGTAAAAG ATACCTGCTT TCTTCAGCCT ATGTAGACAG CCACAAATGG 
GAAGCAAGAG AAAAAGAACA TTACTGTCTT GCTGATCTTG CATCTTTAAT GGATAAAACA 
TTTGAGAGAA AGTTGCCTGT TAGTTCTTTA ACAATATCAC GGCTTATAGA CAACATTTCC 
TCTCGGGAAG AGATAGATCA TGCAGAGTAT TACCTTTACA AAAAGGGAGA ATGCTCATCC 
AGCAATCCAC AGAATTATTC CAGGTTTCGA CACAGCCCCA ACTGCTGGTA CCTGAGAAAC 
TGGACTATCC ACACCTGGAT TAGGCAGTGT CTAAAATATG ATGCACAAGA CAAAGCCCTA 
TATACCCTTG TAAATAAGGT TCAATATGGA ATTTTTCCAG ATAACTTTAC ATTCAATTTA 
CTGATGGATT CTTTCATAAA GAAAGAAAAT TACAAAGATG CTTTATCTGT GGTTTTTGAG 
GTCATGATGC AAGAAGCCTT TGAAGTGCCT TCCACCCAAC TTCTCTCCCT CTATGTTTTA 
TTTCATTGCC TGGCAAAGAA GACAGACTTC AGTTGGGAAG AGGAGAGGAA CTTTGGTGCA 
TCCCTTTTGC TTCCAGGCCT AAAACAAAAG AACTCAGTGG GTTTCAGTTC CCAGTTGTAT 
GGCTATGCAC TTCTTGGGAA GGTGGAGTTG CAGCAAGGGC TACGGGCTGT GTACCACAAC 
ATGCCTCTGA TATGGAAACC AGGCTACCTT GACAGAGCCC TTCAAGTGAT GGAGAAAGTG 
GCTGCCTCCC CAGAAGACAT AAAGCTGTGT AGAGAAGCGC TCGATGTGCT GGGTGCAGTG 
CTGAAGGCTC TGACTTCAGC TGATGGGGCT TCAGAGGAGC AGTCCCAAAA TGATGAAGAC 
AACCAGGGGT CAGAAAAACT GGTGGAGCAG TTAGACATCG AGGAAACAGA GCAGTCCAAG 
CTTCCTCAAT ACCTGGAACG ATTTAAGGCC TTACATTCTA AGCTTCAAGC TCTGGGCAAA 
ATTGAGTCAG AAGGTCTTTT AAGTCTGACC ACCCAGCTTG TCAAGGAAAA ACTCTCCACC 
TGTGAAGCAG AGGACATCGC CACCTATGAG CAGAATCTGC AGCAGTGGCA TCTAGACCTT 
GTACAGTTGA TCCAGAGAGA ACAGCAACAG AGGGAGCAAG CGAAGCAGGA GTACCAGGCT 
CAGAAAGCAG CAAAGGCATC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High MRPS23 expression contributes to hepatocellular carcinoma proliferation and indicates poor survival outcomes.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(7)1010428317709127(2017 Jul)
Pu M,Wang J,Huang Q,Zhao G,Xia C,Shang R,Zhang Z,Bian Z,Yang X,Tao K

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.
FEBS letters586(20)3555-61(2012 Oct)
Davies SM,Lopez Sanchez MI,Narsai R,Shearwood AM,Razif MF,Small ID,Whelan J,Rackham O,Filipovska A

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.
Proceedings of the National Academy of Sciences of the United States of America108(44)17921-6(2011 Nov)
Surovtseva YV,Shutt TE,Cotney J,Cimen H,Chen SY,Koc EC,Shadel GS

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MRPS27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報