TBC1D2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBC1D2B
Entrez Gene ID	23102
Full Name	TBC1 domain family member 2B
General protein information	Preferred Names TBC1 domain family member 2B Names TBC1 domain family member 2B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.3-q25.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_015079.6	NP_055894.6	TBC1 domain family member 2B isoform b
XM_011521387.2	XP_011519689.1	TBC1 domain family member 2B isoform X1
NM_015079.5	NP_055894.6	TBC1 domain family member 2B isoform b
XM_017022022.1	XP_016877511.1	TBC1 domain family member 2B isoform X2
NM_144572.1	NP_653173.1	TBC1 domain family member 2B isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TBC1D2B	XP_002696708.2
Homo sapiens (human)	TBC1D2B	NP_653173.1
Canis lupus familiaris (dog)	TBC1D2B	XP_005618492.1
Caenorhabditis elegans (roundworm)	tbc-2	NP_495156.1
Pan troglodytes (chimpanzee)	TBC1D2B	XP_510522.4
Gallus gallus (chicken)	TBC1D2B	XP_413750.4
Mus musculus (house mouse)	Tbc1d2b	NP_919315.2
	TBC1D2B	XP_001106607.1
Rattus norvegicus (Norway rat)	Tbc1d2b	NP_001101645.1
Danio rerio (zebrafish)	tbc1d2b	XP_002666912.2
Xenopus tropicalis (tropical clawed frog)	tbc1d2b	NP_001039198.1

Gene Ontology
Bibliography

Related articles in PubMed

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.
Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I
Molecular and cellular biology32(9)1733-44(2012 May)

Network organization of the human autophagy system.
Behrends C, Sowa ME, Gygi SP, Harper JW
Nature466(7302)68-76(2010 Jul)

Functional dissection of Rab GTPases involved in primary cilium formation.
Yoshimura S, Egerer J, Fuchs E, Haas AK, Barr FA
The Journal of cell biology178(3)363-9(2007 Jul)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following TBC1D2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06809	NM_015079.6 Latest version!	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu48179	XM_017022022.1 Latest version!	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu06321	NM_144572.1 Latest version!	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu65436	XM_011521387.2 Latest version!	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu06809	NM_015079.5	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBC1D2B ( NM_015079.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_055894.6 Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06809
Clone ID Related Accession (Same CDS sequence)	NM_015079.6 , NM_015079.5
Accession Version	NM_015079.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	TBC1 domain family member 2B isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090260.13, AB028978.1, AC104758.12 and AL109773.1. On Jun 2, 2019 this sequence version replaced NM_015079.5. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region due to the use of an alternate splice site in the terminal exon, compared to variant 1. The resulting protein (isoform b) has a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC152465.1, AB028978.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC 
GGAACCGACG CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055894.6
CDS	60..2804
Translation	MPGAGARAEEGGGGGEGAAQGAAAEPGAGPAREPARLCGYLQKLSGKGPLRGYRSRWFVFDARRCYLYYFKSPQDALPLGHLDIADACFSYQGPDEAAEPGTEPPAHFQVHSAGAVTVLKAPNRQLMTYWLQELQQKRWEYCNSLDMVKWDSRTSPTPGDFPKGLVARDNTDLIYPHPNASAEKARNVLAVETVPGELVGEQAANQPAPGHPNSINFYSLKQWGNELKNSMSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEGNKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEELEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKESLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARSGTDAPTTWRKSGWS

Target ORF information:

RefSeq Version	NM_015079.6
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015079.6

ORF Insert Sequence:

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC 
GGAACCGACG CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D2B ( NM_015079.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055894.6 Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06809
Clone ID Related Accession (Same CDS sequence)	NM_015079.6 , NM_015079.5
Accession Version	NM_015079.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	TBC1 domain family member 2B isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090260.13, AB028978.1, AC104758.12 and AL109773.1. On Jul 10, 2008 this sequence version replaced NM_015079.4. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region due to the use of an alternate splice site in the terminal exon, compared to variant 1. The resulting protein (isoform b) has a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC152465.1, AB028978.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC 
GGAACCGACG CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055894.6
CDS	1..2745
Translation	MPGAGARAEEGGGGGEGAAQGAAAEPGAGPAREPARLCGYLQKLSGKGPLRGYRSRWFVFDARRCYLYYFKSPQDALPLGHLDIADACFSYQGPDEAAEPGTEPPAHFQVHSAGAVTVLKAPNRQLMTYWLQELQQKRWEYCNSLDMVKWDSRTSPTPGDFPKGLVARDNTDLIYPHPNASAEKARNVLAVETVPGELVGEQAANQPAPGHPNSINFYSLKQWGNELKNSMSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEGNKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEELEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKESLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARSGTDAPTTWRKSGWS

Target ORF information:

RefSeq Version	NM_015079.5
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015079.5

ORF Insert Sequence:

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGATCC 
GGAACCGACG CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D2B ( XM_017022022.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877511.1 Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X4, mRNA.

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CloneID	OHu48179
Clone ID Related Accession (Same CDS sequence)	XM_017022022.1
Accession Version	XM_017022022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	TBC1 domain family member 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCTT TCCGTCCTGG GAGAGGACAT AATGATAGTC GGAGGACTGT GTTTTATACC 
AATGAAGAGT GGGAACTTTT AGACCCAACC CCTAAGGACC TAGAGGAGTC CATAGTACAG 
GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA AACAAAGGAG TAACTGGCTC AGGATTCCCC 
TTTGATTTTG GACGTAACCC CTACAAAGGA AAGCGCCCTT TGAAAGACAT AATTGGGTCG 
TACAAAAATC GTCACAGCAG TGGTGACCCT TCAAGTGAAG GCACATCAGG CAGTGGCAGC 
GTCAGCATCA GGAAGCCGGC CTCCGAAATG CAACTGCAGG TCCAGAGCCA GCAGGAAGAG 
CTGGAACAGT TAAAGAAAGA CCTGTCCAGT CAGAAGGAGC TTGTTCGACT GCTCCAGCAG 
ACAGTCCGGT CATCCCAGTA TGACAAGTAT TTCACAAGCA GCCGGCTCTG TGAGGGGGTC 
CCAAAGGACA CGCTCGAGCT TCTGCACCAA AAGGATGATC AGATTCTGGG CCTTACCAGC 
CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG AGTCTTCAGC AGGAAGTAAG GACGCTGAAG 
AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG GGAATGCTCA TGGAGACCAT CCAAGCCAAG 
GACGAGGTCA TCATCAAGCT CAGCGAGGGC GAGGGCAACG GGCCTCCTCC CACCGTGGCG 
CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC AGGGACCAGC TGGAACTGGA CAGGCTGAAA 
GATAATCTAC AGGGGTACAA AACCCAAAAC AAATTTCTAA ATAAGGAGAT TTTGGAACTC 
TCAGCTCTAC GAAGAAATGC AGAAAGGAGA GAGAGGGATC TGATGGCAAA GTATTCTAGC 
CTGGAAGCCA AGCTCTGCCA GATAGAAAGT AAATACCTGA TATTGCTCCA AGAAATGAAG 
ACACCAGTGT GCTCAGAAGA CCAGGGGCCC ACCCGGGAGG TCATAGCCCA GTTGCTGGAG 
GATGCTCTGC AGGTTGAGAG CCAAGAGCAG CCGGAGCAAG CATTTGTTAA ACCTCATCTT 
GTCAGTGAAT ATGATATTTA TGGGTTCAGG ACTGTACCTG AGGATGATGA GGAAGAGAAA 
TTGGTTGCCA AGGTCCGCGC GTTGGATCTG AAGACTCTCT ACCTCACAGA AAACCAGGAA 
GTCTCCACTG GGGTCAAGTG GGAAAACTAT TTTGCAAGTA CAGTGAACAG GGAGATGATG 
TGCTCTCCAG AGTTAAAAAA CCTCATCCGT GCGGGCATTC CCCACGAGCA CCGTTCCAAG 
GTGTGGAAGT GGTGTGTGGA CCGTCACACC AGGAAGTTCA AGGACAACAC TGAGCCTGGC 
CACTTCCAGA CCTTGCTGCA GAAGGCGCTG GAGAAACAGA ACCCAGCCTC CAAGCAGATT 
GAGCTGGACT TGCTGCGAAC TCTGCCCAAC AACAAACATT ACTCCTGCCC CACCTCAGAA 
GGCATACAGA AGTTACGCAA TGTCCTCCTC GCCTTCTCCT GGCGGAATCC AGATATCGGC 
TACTGTCAAG GCCTAAACAG GTTGGTGGCA GTGGCCCTCC TGTACCTGGA ACAAGAAGAT 
GCTTTCTGGT GTCTCGTTAC CATAGTGGAA GTTTTCATGC CTCGAGACTA TTATACAAAG 
ACTCTTTTAG GATCCCAGGT GGACCAGCGG GTGTTCAGAG ACCTTATGAG TGAGAAGCTG 
CCTCGGTTGC ATGGCCACTT TGAACAGTAC AAAGTCGACT ACACTCTCAT CACTTTCAAC 
TGGTTTCTGG TGGTATTTGT GGATAGTGTC GTTAGTGACA TCCTCTTTAA AATATGGGAC 
TCTTTCCTTT ATGAAGGACC AAAGGTTATT TTCCGTTTTG CTCTGGCACT TTTTAAGTAC 
AAGGAAGAGG AGATTTTGAA ATTGCAAGAT TCGATGTCTA TATTTAAGTA TCTCCGCTAC 
TTCACTCGCA CTATCCTTGA TGCTAGGAAG CTGATCAGTA TCTCCTTTGG GGACCTGAAC 
CCTTTCCCCC TACGCCAGAT CCGGAACCGA CGCGCCTACC ACTTGGAGAA AGTCCGGCTG 
GAGCTGACCG AGCTGGAGGC CATCCGTGAG GACTTCCTGC GTGAGCGGGA CACCAGCCCT 
GACAAGGGTG AGCTGGTCAG TGACGAGGAG GAGGATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877511.1
CDS	712..2913
Translation	MSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEGNKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEELEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKESLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARKLISISFGDLNPFPLRQIRNRRAYHLEKVRLELTELEAIREDFLRERDTSPDKGELVSDEEEDT

Target ORF information:

RefSeq Version	XM_017022022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022022.1

ORF Insert Sequence:

ATGTCTTCTT TCCGTCCTGG GAGAGGACAT AATGATAGTC GGAGGACTGT GTTTTATACC 
AATGAAGAGT GGGAACTTTT AGACCCAACC CCTAAGGACC TAGAGGAGTC CATAGTACAG 
GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA AACAAAGGAG TAACTGGCTC AGGATTCCCC 
TTTGATTTTG GACGTAACCC CTACAAAGGA AAGCGCCCTT TGAAAGACAT AATTGGGTCG 
TACAAAAATC GTCACAGCAG TGGTGACCCT TCAAGTGAAG GCACATCAGG CAGTGGCAGC 
GTCAGCATCA GGAAGCCGGC CTCCGAAATG CAACTGCAGG TCCAGAGCCA GCAGGAAGAG 
CTGGAACAGT TAAAGAAAGA CCTGTCCAGT CAGAAGGAGC TTGTTCGACT GCTCCAGCAG 
ACAGTCCGGT CATCCCAGTA TGACAAGTAT TTCACAAGCA GCCGGCTCTG TGAGGGGGTC 
CCAAAGGACA CGCTCGAGCT TCTGCACCAA AAGGATGATC AGATTCTGGG CCTTACCAGC 
CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG AGTCTTCAGC AGGAAGTAAG GACGCTGAAG 
AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG GGAATGCTCA TGGAGACCAT CCAAGCCAAG 
GACGAGGTCA TCATCAAGCT CAGCGAGGGC GAGGGCAACG GGCCTCCTCC CACCGTGGCG 
CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC AGGGACCAGC TGGAACTGGA CAGGCTGAAA 
GATAATCTAC AGGGGTACAA AACCCAAAAC AAATTTCTAA ATAAGGAGAT TTTGGAACTC 
TCAGCTCTAC GAAGAAATGC AGAAAGGAGA GAGAGGGATC TGATGGCAAA GTATTCTAGC 
CTGGAAGCCA AGCTCTGCCA GATAGAAAGT AAATACCTGA TATTGCTCCA AGAAATGAAG 
ACACCAGTGT GCTCAGAAGA CCAGGGGCCC ACCCGGGAGG TCATAGCCCA GTTGCTGGAG 
GATGCTCTGC AGGTTGAGAG CCAAGAGCAG CCGGAGCAAG CATTTGTTAA ACCTCATCTT 
GTCAGTGAAT ATGATATTTA TGGGTTCAGG ACTGTACCTG AGGATGATGA GGAAGAGAAA 
TTGGTTGCCA AGGTCCGCGC GTTGGATCTG AAGACTCTCT ACCTCACAGA AAACCAGGAA 
GTCTCCACTG GGGTCAAGTG GGAAAACTAT TTTGCAAGTA CAGTGAACAG GGAGATGATG 
TGCTCTCCAG AGTTAAAAAA CCTCATCCGT GCGGGCATTC CCCACGAGCA CCGTTCCAAG 
GTGTGGAAGT GGTGTGTGGA CCGTCACACC AGGAAGTTCA AGGACAACAC TGAGCCTGGC 
CACTTCCAGA CCTTGCTGCA GAAGGCGCTG GAGAAACAGA ACCCAGCCTC CAAGCAGATT 
GAGCTGGACT TGCTGCGAAC TCTGCCCAAC AACAAACATT ACTCCTGCCC CACCTCAGAA 
GGCATACAGA AGTTACGCAA TGTCCTCCTC GCCTTCTCCT GGCGGAATCC AGATATCGGC 
TACTGTCAAG GCCTAAACAG GTTGGTGGCA GTGGCCCTCC TGTACCTGGA ACAAGAAGAT 
GCTTTCTGGT GTCTCGTTAC CATAGTGGAA GTTTTCATGC CTCGAGACTA TTATACAAAG 
ACTCTTTTAG GATCCCAGGT GGACCAGCGG GTGTTCAGAG ACCTTATGAG TGAGAAGCTG 
CCTCGGTTGC ATGGCCACTT TGAACAGTAC AAAGTCGACT ACACTCTCAT CACTTTCAAC 
TGGTTTCTGG TGGTATTTGT GGATAGTGTC GTTAGTGACA TCCTCTTTAA AATATGGGAC 
TCTTTCCTTT ATGAAGGACC AAAGGTTATT TTCCGTTTTG CTCTGGCACT TTTTAAGTAC 
AAGGAAGAGG AGATTTTGAA ATTGCAAGAT TCGATGTCTA TATTTAAGTA TCTCCGCTAC 
TTCACTCGCA CTATCCTTGA TGCTAGGAAG CTGATCAGTA TCTCCTTTGG GGACCTGAAC 
CCTTTCCCCC TACGCCAGAT CCGGAACCGA CGCGCCTACC ACTTGGAGAA AGTCCGGCTG 
GAGCTGACCG AGCTGGAGGC CATCCGTGAG GACTTCCTGC GTGAGCGGGA CACCAGCCCT 
GACAAGGGTG AGCTGGTCAG TGACGAGGAG GAGGATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D2B ( NM_144572.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_653173.1 Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 1, mRNA.

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CloneID	OHu06321
Clone ID Related Accession (Same CDS sequence)	NM_144572.1
Accession Version	NM_144572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	TBC1 domain family member 2B isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090260.13, AB028978.1, BC140766.1, AC104758.12 and AL109773.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.138483.1, SRR1803614.83206.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGGAAG 
CTGATCAGTA TCTCCTTTGG GGACCTGAAC CCTTTCCCCC TACGCCAGAT CCGGAACCGA 
CGCGCCTACC ACTTGGAGAA AGTCCGGCTG GAGCTGACCG AGCTGGAGGC CATCCGTGAG 
GACTTCCTGC GTGAGCGGGA CACCAGCCCT GACAAGGGTG AGCTGGTCAG TGACGAGGAG 
GAGGATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653173.1
CDS	1..2892
Translation	MPGAGARAEEGGGGGEGAAQGAAAEPGAGPAREPARLCGYLQKLSGKGPLRGYRSRWFVFDARRCYLYYFKSPQDALPLGHLDIADACFSYQGPDEAAEPGTEPPAHFQVHSAGAVTVLKAPNRQLMTYWLQELQQKRWEYCNSLDMVKWDSRTSPTPGDFPKGLVARDNTDLIYPHPNASAEKARNVLAVETVPGELVGEQAANQPAPGHPNSINFYSLKQWGNELKNSMSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEGNKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEELEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKESLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARKLISISFGDLNPFPLRQIRNRRAYHLEKVRLELTELEAIREDFLRERDTSPDKGELVSDEEEDT

Target ORF information:

RefSeq Version	NM_144572.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144572.1

ORF Insert Sequence:

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA GATAATCTAC AGGGGTACAA AACCCAAAAC 
AAATTTCTAA ATAAGGAGAT TTTGGAACTC TCAGCTCTAC GAAGAAATGC AGAAAGGAGA 
GAGAGGGATC TGATGGCAAA GTATTCTAGC CTGGAAGCCA AGCTCTGCCA GATAGAAAGT 
AAATACCTGA TATTGCTCCA AGAAATGAAG ACACCAGTGT GCTCAGAAGA CCAGGGGCCC 
ACCCGGGAGG TCATAGCCCA GTTGCTGGAG GATGCTCTGC AGGTTGAGAG CCAAGAGCAG 
CCGGAGCAAG CATTTGTTAA ACCTCATCTT GTCAGTGAAT ATGATATTTA TGGGTTCAGG 
ACTGTACCTG AGGATGATGA GGAAGAGAAA TTGGTTGCCA AGGTCCGCGC GTTGGATCTG 
AAGACTCTCT ACCTCACAGA AAACCAGGAA GTCTCCACTG GGGTCAAGTG GGAAAACTAT 
TTTGCAAGTA CAGTGAACAG GGAGATGATG TGCTCTCCAG AGTTAAAAAA CCTCATCCGT 
GCGGGCATTC CCCACGAGCA CCGTTCCAAG GTGTGGAAGT GGTGTGTGGA CCGTCACACC 
AGGAAGTTCA AGGACAACAC TGAGCCTGGC CACTTCCAGA CCTTGCTGCA GAAGGCGCTG 
GAGAAACAGA ACCCAGCCTC CAAGCAGATT GAGCTGGACT TGCTGCGAAC TCTGCCCAAC 
AACAAACATT ACTCCTGCCC CACCTCAGAA GGCATACAGA AGTTACGCAA TGTCCTCCTC 
GCCTTCTCCT GGCGGAATCC AGATATCGGC TACTGTCAAG GCCTAAACAG GTTGGTGGCA 
GTGGCCCTCC TGTACCTGGA ACAAGAAGAT GCTTTCTGGT GTCTCGTTAC CATAGTGGAA 
GTTTTCATGC CTCGAGACTA TTATACAAAG ACTCTTTTAG GATCCCAGGT GGACCAGCGG 
GTGTTCAGAG ACCTTATGAG TGAGAAGCTG CCTCGGTTGC ATGGCCACTT TGAACAGTAC 
AAAGTCGACT ACACTCTCAT CACTTTCAAC TGGTTTCTGG TGGTATTTGT GGATAGTGTC 
GTTAGTGACA TCCTCTTTAA AATATGGGAC TCTTTCCTTT ATGAAGGACC AAAGGTTATT 
TTCCGTTTTG CTCTGGCACT TTTTAAGTAC AAGGAAGAGG AGATTTTGAA ATTGCAAGAT 
TCGATGTCTA TATTTAAGTA TCTCCGCTAC TTCACTCGCA CTATCCTTGA TGCTAGGAAG 
CTGATCAGTA TCTCCTTTGG GGACCTGAAC CCTTTCCCCC TACGCCAGAT CCGGAACCGA 
CGCGCCTACC ACTTGGAGAA AGTCCGGCTG GAGCTGACCG AGCTGGAGGC CATCCGTGAG 
GACTTCCTGC GTGAGCGGGA CACCAGCCCT GACAAGGGTG AGCTGGTCAG TGACGAGGAG 
GAGGATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D2B ( XM_011521387.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519689.1 Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X1, mRNA.

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CloneID	OHu65436
Clone ID Related Accession (Same CDS sequence)	XM_011521387.2
Accession Version	XM_011521387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	TBC1 domain family member 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA TATTCTAGCC TGGAAGCCAA GCTCTGCCAG 
ATAGAAAGTA AATACCTGAT ATTGCTCCAA GAAATGAAGA CACCAGTGTG CTCAGAAGAC 
CAGGGGCCCA CCCGGGAGGT CATAGCCCAG TTGCTGGAGG ATGCTCTGCA GGTTGAGAGC 
CAAGAGCAGC CGGAGCAAGC ATTTGTTAAA CCTCATCTTG TCAGTGAATA TGATATTTAT 
GGGTTCAGGA CTGTACCTGA GGATGATGAG GAAGAGAAAT TGGTTGCCAA GGTCCGCGCG 
TTGGATCTGA AGACTCTCTA CCTCACAGAA AACCAGGAAG TCTCCACTGG GGTCAAGTGG 
GAAAACTATT TTGCAAGTAC AGTGAACAGG GAGATGATGT GCTCTCCAGA GTTAAAAAAC 
CTCATCCGTG CGGGCATTCC CCACGAGCAC CGTTCCAAGG TGTGGAAGTG GTGTGTGGAC 
CGTCACACCA GGAAGTTCAA GGACAACACT GAGCCTGGCC ACTTCCAGAC CTTGCTGCAG 
AAGGCGCTGG AGAAACAGAA CCCAGCCTCC AAGCAGATTG AGCTGGACTT GCTGCGAACT 
CTGCCCAACA ACAAACATTA CTCCTGCCCC ACCTCAGAAG GCATACAGAA GTTACGCAAT 
GTCCTCCTCG CCTTCTCCTG GCGGAATCCA GATATCGGCT ACTGTCAAGG CCTAAACAGG 
TTGGTGGCAG TGGCCCTCCT GTACCTGGAA CAAGAAGATG CTTTCTGGTG TCTCGTTACC 
ATAGTGGAAG TTTTCATGCC TCGAGACTAT TATACAAAGA CTCTTTTAGG ATCCCAGGTG 
GACCAGCGGG TGTTCAGAGA CCTTATGAGT GAGAAGCTGC CTCGGTTGCA TGGCCACTTT 
GAACAGTACA AAGTCGACTA CACTCTCATC ACTTTCAACT GGTTTCTGGT GGTATTTGTG 
GATAGTGTCG TTAGTGACAT CCTCTTTAAA ATATGGGACT CTTTCCTTTA TGAAGGACCA 
AAGGTTATTT TCCGTTTTGC TCTGGCACTT TTTAAGTACA AGGAAGAGGA GATTTTGAAA 
TTGCAAGATT CGATGTCTAT ATTTAAGTAT CTCCGCTACT TCACTCGCAC TATCCTTGAT 
GCTAGGAAGC TGATCAGTAT CTCCTTTGGG GACCTGAACC CTTTCCCCCT ACGCCAGATC 
CGGAACCGAC GCGCCTACCA CTTGGAGAAA GTCCGGCTGG AGCTGACCGA GCTGGAGGCC 
ATCCGTGAGG ACTTCCTGCG TGAGCGGGAC ACCAGCCCTG ACAAGGGTGA GCTGGTCAGT 
GACGAGGAGG AGGATACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519689.1
CDS	57..2837
Translation	MPGAGARAEEGGGGGEGAAQGAAAEPGAGPAREPARLCGYLQKLSGKGPLRGYRSRWFVFDARRCYLYYFKSPQDALPLGHLDIADACFSYQGPDEAAEPGTEPPAHFQVHSAGAVTVLKAPNRQLMTYWLQELQQKRWEYCNSLDMVKWDSRTSPTPGDFPKGLVARDNTDLIYPHPNASAEKARNVLAVETVPGELVGEQAANQPAPGHPNSINFYSLKQWGNELKNSMSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEGNKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEELEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKESLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARKLISISFGDLNPFPLRQIRNRRAYHLEKVRLELTELEAIREDFLRERDTSPDKGELVSDEEEDT

Target ORF information:

RefSeq Version	XM_011521387.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TBC1 domain family member 2B (TBC1D2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521387.2

ORF Insert Sequence:

ATGCCGGGGG CCGGAGCCCG GGCGGAGGAG GGCGGCGGCG GCGGCGAGGG CGCGGCGCAG 
GGGGCGGCCG CGGAGCCCGG GGCGGGTCCG GCGCGGGAGC CAGCGCGGCT GTGTGGCTAT 
CTGCAGAAGC TGTCGGGCAA GGGCCCCCTG CGTGGCTACC GCAGCCGCTG GTTCGTGTTC 
GACGCGCGCC GCTGCTACCT TTACTATTTC AAGAGTCCGC AGGACGCGCT GCCCCTCGGC 
CACTTGGACA TCGCGGACGC CTGCTTCAGC TACCAGGGCC CCGACGAGGC GGCGGAGCCG 
GGCACGGAGC CGCCCGCGCA CTTCCAGGTG CACAGCGCGG GAGCCGTCAC GGTGCTCAAG 
GCTCCCAATC GTCAACTCAT GACTTACTGG TTACAGGAGC TTCAGCAGAA GAGATGGGAA 
TATTGTAACA GTCTTGACAT GGTCAAGTGG GACAGCAGGA CCTCTCCAAC TCCCGGGGAT 
TTTCCTAAGG GTCTTGTAGC CAGAGATAAC ACTGATTTAA TTTACCCACA CCCAAATGCT 
TCTGCAGAAA AAGCCAGAAA TGTCCTAGCT GTGGAGACTG TGCCTGGAGA GCTGGTGGGA 
GAACAAGCTG CAAATCAGCC CGCCCCAGGG CATCCAAATT CCATTAATTT TTACTCTTTG 
AAACAGTGGG GCAATGAGCT CAAGAATTCG ATGTCTTCTT TCCGTCCTGG GAGAGGACAT 
AATGATAGTC GGAGGACTGT GTTTTATACC AATGAAGAGT GGGAACTTTT AGACCCAACC 
CCTAAGGACC TAGAGGAGTC CATAGTACAG GAAGAAAAGA AGAAGCTGAC CCCTGAAGGA 
AACAAAGGAG TAACTGGCTC AGGATTCCCC TTTGATTTTG GACGTAACCC CTACAAAGGA 
AAGCGCCCTT TGAAAGACAT AATTGGGTCG TACAAAAATC GTCACAGCAG TGGTGACCCT 
TCAAGTGAAG GCACATCAGG CAGTGGCAGC GTCAGCATCA GGAAGCCGGC CTCCGAAATG 
CAACTGCAGG TCCAGAGCCA GCAGGAAGAG CTGGAACAGT TAAAGAAAGA CCTGTCCAGT 
CAGAAGGAGC TTGTTCGACT GCTCCAGCAG ACAGTCCGGT CATCCCAGTA TGACAAGTAT 
TTCACAAGCA GCCGGCTCTG TGAGGGGGTC CCAAAGGACA CGCTCGAGCT TCTGCACCAA 
AAGGATGATC AGATTCTGGG CCTTACCAGC CAGCTGGAGA GGTTCAGCTT GGAGAAGGAG 
AGTCTTCAGC AGGAAGTAAG GACGCTGAAG AGCAAAGTGG GCGAGCTCAA CGAGCAGCTG 
GGAATGCTCA TGGAGACCAT CCAAGCCAAG GACGAGGTCA TCATCAAGCT CAGCGAGGGC 
GAGGGCAACG GGCCTCCTCC CACCGTGGCG CCCAGCTCCC CTTCGGTTGT GCCTGTTGCC 
AGGGACCAGC TGGAACTGGA CAGGCTGAAA TATTCTAGCC TGGAAGCCAA GCTCTGCCAG 
ATAGAAAGTA AATACCTGAT ATTGCTCCAA GAAATGAAGA CACCAGTGTG CTCAGAAGAC 
CAGGGGCCCA CCCGGGAGGT CATAGCCCAG TTGCTGGAGG ATGCTCTGCA GGTTGAGAGC 
CAAGAGCAGC CGGAGCAAGC ATTTGTTAAA CCTCATCTTG TCAGTGAATA TGATATTTAT 
GGGTTCAGGA CTGTACCTGA GGATGATGAG GAAGAGAAAT TGGTTGCCAA GGTCCGCGCG 
TTGGATCTGA AGACTCTCTA CCTCACAGAA AACCAGGAAG TCTCCACTGG GGTCAAGTGG 
GAAAACTATT TTGCAAGTAC AGTGAACAGG GAGATGATGT GCTCTCCAGA GTTAAAAAAC 
CTCATCCGTG CGGGCATTCC CCACGAGCAC CGTTCCAAGG TGTGGAAGTG GTGTGTGGAC 
CGTCACACCA GGAAGTTCAA GGACAACACT GAGCCTGGCC ACTTCCAGAC CTTGCTGCAG 
AAGGCGCTGG AGAAACAGAA CCCAGCCTCC AAGCAGATTG AGCTGGACTT GCTGCGAACT 
CTGCCCAACA ACAAACATTA CTCCTGCCCC ACCTCAGAAG GCATACAGAA GTTACGCAAT 
GTCCTCCTCG CCTTCTCCTG GCGGAATCCA GATATCGGCT ACTGTCAAGG CCTAAACAGG 
TTGGTGGCAG TGGCCCTCCT GTACCTGGAA CAAGAAGATG CTTTCTGGTG TCTCGTTACC 
ATAGTGGAAG TTTTCATGCC TCGAGACTAT TATACAAAGA CTCTTTTAGG ATCCCAGGTG 
GACCAGCGGG TGTTCAGAGA CCTTATGAGT GAGAAGCTGC CTCGGTTGCA TGGCCACTTT 
GAACAGTACA AAGTCGACTA CACTCTCATC ACTTTCAACT GGTTTCTGGT GGTATTTGTG 
GATAGTGTCG TTAGTGACAT CCTCTTTAAA ATATGGGACT CTTTCCTTTA TGAAGGACCA 
AAGGTTATTT TCCGTTTTGC TCTGGCACTT TTTAAGTACA AGGAAGAGGA GATTTTGAAA 
TTGCAAGATT CGATGTCTAT ATTTAAGTAT CTCCGCTACT TCACTCGCAC TATCCTTGAT 
GCTAGGAAGC TGATCAGTAT CTCCTTTGGG GACCTGAACC CTTTCCCCCT ACGCCAGATC 
CGGAACCGAC GCGCCTACCA CTTGGAGAAA GTCCGGCTGG AGCTGACCGA GCTGGAGGCC 
ATCCGTGAGG ACTTCCTGCG TGAGCGGGAC ACCAGCCCTG ACAAGGGTGA GCTGGTCAGT 
GACGAGGAGG AGGATACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.
Molecular and cellular biology32(9)1733-44(2012 May)
Popovic D,Akutsu M,Novak I,Harper JW,Behrends C,Dikic I

Network organization of the human autophagy system.
Nature466(7302)68-76(2010 Jul)
Behrends C,Sowa ME,Gygi SP,Harper JW

Functional dissection of Rab GTPases involved in primary cilium formation.
The Journal of cell biology178(3)363-9(2007 Jul)
Yoshimura S,Egerer J,Fuchs E,Haas AK,Barr FA

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

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TBC1D2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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