WAPL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WAPL
Entrez Gene ID	23063
Full Name	WAPL cohesin release factor
Synonyms	FOE,KIAA0261,WAPAL
General protein information	Preferred Names WAPL cohesin release factor Names wings apart-like protein homolog friend of EBNA2 (Epstein-Barr virus nuclear protein 2) friend of EBNA2 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.2
Disorder MIM:	610754

mRNA and Protein(s)

mRNA	Protein	Name
NM_015045.3	NP_055860.1	wings apart-like protein homolog isoform 1
XM_017015980.1	XP_016871469.1	wings apart-like protein homolog isoform X3
NM_015045.5	NP_055860.1	wings apart-like protein homolog isoform 1
NM_015045.4	NP_055860.1	wings apart-like protein homolog isoform 1
XM_011539548.1	XP_011537850.1	wings apart-like protein homolog isoform X1
XM_017015979.1	XP_016871468.1	wings apart-like protein homolog isoform X2
XM_017015981.1	XP_016871470.1	wings apart-like protein homolog isoform X4
NM_001318328.1	NP_001305257.1	wings apart-like protein homolog isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	WAPAL	NP_055860.1
Pan troglodytes (chimpanzee)	WAPAL	XP_003312721.1
	WAPAL	XP_002805691.1
Canis lupus familiaris (dog)	WAPAL	XP_864834.3
Mus musculus (house mouse)	Wapal	NP_001004436.2
Gallus gallus (chicken)	WAPAL	NP_001186440.1
Danio rerio (zebrafish)	wapala	XP_688605.5
Xenopus tropicalis (tropical clawed frog)	wapal	XP_002937603.2
Bos taurus (cattle)	WAPAL	XP_005226580.1
Caenorhabditis elegans (roundworm)	wapl-1	NP_500567.1
Rattus norvegicus (Norway rat)	Wapal	XP_006252785.1

Gene Ontology
Bibliography

Related articles in PubMed

A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion.
Liang C, Chen Q, Yi Q, Zhang M, Yan H, Zhang B, Zhou L, Zhang Z, Qi F, Ye S, Wang F
EMBO reports19(1)43-56(2018 Jan)

Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.
Wutz G, V?rnai C, Nagasaka K, Cisneros DA, Stocsits RR, Tang W, Schoenfelder S, Jessberger G, Muhar M, Hossain MJ, Walther N, Koch B, Kueblbeck M, Ellenberg J, Zuber J, Fraser P, Peters JM
The EMBO journal36(24)3573-3599(2017 12)

The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension.
Haarhuis JHI, van der Weide RH, Blomen VA, Y??ez-Cuna JO, Amendola M, van Ruiten MS, Krijger PHL, Teunissen H, Medema RH, van Steensel B, Brummelkamp TR, de Wit E, Rowland BD
Cell169(4)693-707.e14(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL.
Title: Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.

Study shows that chromatin loop size can be increased and that the duration with which cohesin embraces DNA determines the degree to which loops are enlarged. Cohesin's DNA release factor WAPL restricts this loop extension and also prevents looping between incorrectly oriented CTCF sites.
Title: The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension.

Rs7083506 and rs11202058 polymorphisms of hWAPL and their haplotype T-A were associated with cervical cancer.
Title: Relationship between hWAPL polymorphisms and cervical cancer susceptibility.

WAPL-depleted cells undergo anaphase with segregation errors, resulting in micronuclei and DNA damage. Stable WAPL depletion arrests cells in a p53-dependent manner but causes p53-deficient cells to become highly aneuploid.
Title: WAPL-mediated removal of cohesin protects against segregation errors and aneuploidy.

Wapl-C interacts with other cohesin subunits and possibly unknown effectors to trigger cohesin release from chromatin
Title: Structure of the human cohesin inhibitor Wapl.

The following WAPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu91236	XM_017015981.1 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu06785	NM_015045.5 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu91235	XM_017015980.1 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44161	NM_001318328.1 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu65376	XM_017015979.1 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65377	XM_011539548.1 Latest version!	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu06785	NM_015045.3	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu06785	NM_015045.4	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WAPL ( XM_017015981.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871470.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91236
Clone ID Related Accession (Same CDS sequence)	XM_017015981.1
Accession Version	XM_017015981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCCA CAAAAGCTGT ATATAATGCC AGACATTGGA ATCATCCAGA TTCAGAAGAA 
CTGCCTGGGC CACCAGTAGT AAAACCTCAG AGTGTCACAG TGAGGCTGTC TTCAAAGGAA 
CCAAATCAAA AAGATGATGG AGTTTTTAAG GCTCCTGCAC CACCATCCAA AGTGATAAAA 
ACTGTGACAA TACCTACTCA GCCCTACCAA GATATAGTTA CTGCACTGAA ATGCAGACGA 
GAAGACAAAG AATTATATAC TGTTGTTCAG CACGTGAAGC ACTTCAACGA TGTTGTAGAA 
TTTGGTGAAA ATCAAGAGTT CACTGATGAC ATTGAGTACT TGTTAAGTGG CTTAAAGAGC 
ACTCAGCCTC TAAACACACG TTGCCTTAGT GTTATTAGCT TGGCTACTAA ATGTGCCATG 
CCCAGTTTTC GAATGCACCT GAGAGCACAT GGGATGGTAG CAATGGTCTT TAAAACCTTG 
GATGATTCCC AGCACCATCA GAATCTGTCC CTCTGTACAG CTGCCCTCAT GTATATACTG 
AGTAGAGATC GTTTGAACAT GGATCTTGAT AGAGCTAGCT TAGATCTAAT GATTCGACTT 
TTGGAACTGG AACAAGATGC TTCATCAGCC AAGCTACTGA ATGAAAAAGA CATGAACAAA 
ATTAAAGAAA AAATCCGAAG GCTCTGTGAA ACTGTACACA ACAAGCATCT TGATCTAGAA 
AATATAACGA CTGGGCATTT AGCTATGGAG ACATTATTAT CCCTTACTTC TAAACGAGCA 
GGAGACTGGT TTAAAGAAGA ACTCCGGCTT TTGGGTGGTC TGGATCATAT TGTAGATAAA 
GTAAAAGAAT GTGTGGATCA TTTAAGTAGA GATGAGGATG AAGAGAAACT GGTAGCCTCA 
CTATGGGGAG CAGAGAGATG TTTACGAGTT TTAGAAAGTG TAACTGTGCA TAATCCCGAA 
AATCAAAGCT ACTTGATAGC ATATAAAGAT TCCCAACTTA TTGTTTCATC AGCTAAAGCA 
TTACAGCATT GTGAAGAACT GATTCAGCAG TACAACCGTG CTGAGGACAG CATATGCTTA 
GCTGACAGTA AGCCTCTGCC TCACCAGAAT GTAACTAACC ATGTAGGCAA AGCAGTGGAG 
GACTGCATGA GGGCCATCAT CGGGGTGTTG CTTAATTTAA CTAATGATAA TGAGTGGGGC 
AGCACCAAAA CAGGAGAGCA GGACGGTCTC ATAGGCACAG CGCTGAACTG TGTGCTTCAG 
GTTCCAAAGT ACCTACCTCA GGAGCAGAGA TTTGATATTC GAGTGCTGGG CTTAGGTCTG 
CTGATAAATC TAGTGGAGTA TAGTGCTCGG AATCGGCACT GTCTTGTCAA CATGGAAACA 
TCGTGCTCTT TTGATTCTTC CATCTGTAGT GGAGAAGGGG ATGATAGTTT AAGGATAGGT 
GGACAAGTTC ATGCTGTCCA GGCTTTAGTG CAGCTATTCC TTGAGCGAGA GCGGGCAGCC 
CAGCTAGCAG AAAGTAAAAC AGATGAGTTG ATCAAAGATG CTCCCACCAC TCAGCATGAT 
AAGAGTGGAG AGTGGCAAGA AACAAGTGGA GAAATACAGT GGGTGTCAAC TGAAAAGACT 
GATGGTACAG AAGAGAAACA TAAGAAGGAG GAGGAGGATG AAGAACTTGA CCTCAATAAA 
GCCCTTCAGC ATGCCGGCAA ACACATGGAG GATTGCATTG TGGCCTCCTA CACAGCACTA 
CTTCTTGGGT GTCTCTGCCA GGAAAGTCCA ATCAATGTAA CCACTGTGCG GGAATATCTG 
CCAGAAGGAG ACTTTTCAAT AATGACAGAG ATGCTCAAAA AATTTTTGAG TTTTATGAAT 
CTCACTTGTG CTGTTGGAAC AACTGGCCAG AAATCTATCT CTAGAGTGAT TGAATATTTG 
GAACATTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871470.1
CDS	1671..3602
Translation	MSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_017015981.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015981.1

ORF Insert Sequence:

ATGTCACCCA CAAAAGCTGT ATATAATGCC AGACATTGGA ATCATCCAGA TTCAGAAGAA 
CTGCCTGGGC CACCAGTAGT AAAACCTCAG AGTGTCACAG TGAGGCTGTC TTCAAAGGAA 
CCAAATCAAA AAGATGATGG AGTTTTTAAG GCTCCTGCAC CACCATCCAA AGTGATAAAA 
ACTGTGACAA TACCTACTCA GCCCTACCAA GATATAGTTA CTGCACTGAA ATGCAGACGA 
GAAGACAAAG AATTATATAC TGTTGTTCAG CACGTGAAGC ACTTCAACGA TGTTGTAGAA 
TTTGGTGAAA ATCAAGAGTT CACTGATGAC ATTGAGTACT TGTTAAGTGG CTTAAAGAGC 
ACTCAGCCTC TAAACACACG TTGCCTTAGT GTTATTAGCT TGGCTACTAA ATGTGCCATG 
CCCAGTTTTC GAATGCACCT GAGAGCACAT GGGATGGTAG CAATGGTCTT TAAAACCTTG 
GATGATTCCC AGCACCATCA GAATCTGTCC CTCTGTACAG CTGCCCTCAT GTATATACTG 
AGTAGAGATC GTTTGAACAT GGATCTTGAT AGAGCTAGCT TAGATCTAAT GATTCGACTT 
TTGGAACTGG AACAAGATGC TTCATCAGCC AAGCTACTGA ATGAAAAAGA CATGAACAAA 
ATTAAAGAAA AAATCCGAAG GCTCTGTGAA ACTGTACACA ACAAGCATCT TGATCTAGAA 
AATATAACGA CTGGGCATTT AGCTATGGAG ACATTATTAT CCCTTACTTC TAAACGAGCA 
GGAGACTGGT TTAAAGAAGA ACTCCGGCTT TTGGGTGGTC TGGATCATAT TGTAGATAAA 
GTAAAAGAAT GTGTGGATCA TTTAAGTAGA GATGAGGATG AAGAGAAACT GGTAGCCTCA 
CTATGGGGAG CAGAGAGATG TTTACGAGTT TTAGAAAGTG TAACTGTGCA TAATCCCGAA 
AATCAAAGCT ACTTGATAGC ATATAAAGAT TCCCAACTTA TTGTTTCATC AGCTAAAGCA 
TTACAGCATT GTGAAGAACT GATTCAGCAG TACAACCGTG CTGAGGACAG CATATGCTTA 
GCTGACAGTA AGCCTCTGCC TCACCAGAAT GTAACTAACC ATGTAGGCAA AGCAGTGGAG 
GACTGCATGA GGGCCATCAT CGGGGTGTTG CTTAATTTAA CTAATGATAA TGAGTGGGGC 
AGCACCAAAA CAGGAGAGCA GGACGGTCTC ATAGGCACAG CGCTGAACTG TGTGCTTCAG 
GTTCCAAAGT ACCTACCTCA GGAGCAGAGA TTTGATATTC GAGTGCTGGG CTTAGGTCTG 
CTGATAAATC TAGTGGAGTA TAGTGCTCGG AATCGGCACT GTCTTGTCAA CATGGAAACA 
TCGTGCTCTT TTGATTCTTC CATCTGTAGT GGAGAAGGGG ATGATAGTTT AAGGATAGGT 
GGACAAGTTC ATGCTGTCCA GGCTTTAGTG CAGCTATTCC TTGAGCGAGA GCGGGCAGCC 
CAGCTAGCAG AAAGTAAAAC AGATGAGTTG ATCAAAGATG CTCCCACCAC TCAGCATGAT 
AAGAGTGGAG AGTGGCAAGA AACAAGTGGA GAAATACAGT GGGTGTCAAC TGAAAAGACT 
GATGGTACAG AAGAGAAACA TAAGAAGGAG GAGGAGGATG AAGAACTTGA CCTCAATAAA 
GCCCTTCAGC ATGCCGGCAA ACACATGGAG GATTGCATTG TGGCCTCCTA CACAGCACTA 
CTTCTTGGGT GTCTCTGCCA GGAAAGTCCA ATCAATGTAA CCACTGTGCG GGAATATCTG 
CCAGAAGGAG ACTTTTCAAT AATGACAGAG ATGCTCAAAA AATTTTTGAG TTTTATGAAT 
CTCACTTGTG CTGTTGGAAC AACTGGCCAG AAATCTATCT CTAGAGTGAT TGAATATTTG 
GAACATTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( NM_015045.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055860.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06785
Clone ID Related Accession (Same CDS sequence)	NM_015045.3 , NM_015045.5 , NM_015045.4
Accession Version	NM_015045.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731569.18 and AL844892.5. On Aug 31, 2019 this sequence version replaced NM_015045.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB065003.1, SRR1660803.171190.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000298767.10/ ENSP00000298767.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055860.1
CDS	451..4023
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_015045.5
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015045.5

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_017015980.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871469.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.

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CloneID	OHu91235
Clone ID Related Accession (Same CDS sequence)	XM_017015980.1
Accession Version	XM_017015980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
TTATATACTG TTGTTCAGCA CGTGAAGCAC TTCAACGATG TTGTAGAATT TGGTGAAAAT 
CAAGAGTTCA CTGATGACAT TGAGTACTTG TTAAGTGGCT TAAAGAGCAC TCAGCCTCTA 
AACACACGTT GCCTTAGTGT TATTAGCTTG GCTACTAAAT GTGCCATGCC CAGTTTTCGA 
ATGCACCTGA GAGCACATGG GATGGTAGCA ATGGTCTTTA AAACCTTGGA TGATTCCCAG 
CACCATCAGA ATCTGTCCCT CTGTACAGCT GCCCTCATGT ATATACTGAG TAGAGATCGT 
TTGAACATGG ATCTTGATAG AGCTAGCTTA GATCTAATGA TTCGACTTTT GGAACTGGAA 
CAAGATGCTT CATCAGCCAA GCTACTGAAT GAAAAAGACA TGAACAAAAT TAAAGAAAAA 
ATCCGAAGGC TCTGTGAAAC TGTACACAAC AAGCATCTTG ATCTAGAAAA TATAACGACT 
GGGCATTTAG CTATGGAGAC ATTATTATCC CTTACTTCTA AACGAGCAGG AGACTGGTTT 
AAAGAAGAAC TCCGGCTTTT GGGTGGTCTG GATCATATTG TAGATAAAGT AAAAGAATGT 
GTGGATCATT TAAGTAGAGA TGAGGATGAA GAGAAACTGG TAGCCTCACT ATGGGGAGCA 
GAGAGATGTT TACGAGTTTT AGAAAGTGTA ACTGTGCATA ATCCCGAAAA TCAAAGCTAC 
TTGATAGCAT ATAAAGATTC CCAACTTATT GTTTCATCAG CTAAAGCATT ACAGCATTGT 
GAAGAACTGA TTCAGCAGTA CAACCGTGCT GAGGACAGCA TATGCTTAGC TGACAGTAAG 
CCTCTGCCTC ACCAGAATGT AACTAACCAT GTAGGCAAAG CAGTGGAGGA CTGCATGAGG 
GCCATCATCG GGGTGTTGCT TAATTTAACT AATGATAATG AGTGGGGCAG CACCAAAACA 
GGAGAGCAGG ACGGTCTCAT AGGCACAGCG CTGAACTGTG TGCTTCAGGT TCCAAAGTAC 
CTACCTCAGG AGCAGAGATT TGATATTCGA GTGCTGGGCT TAGGTCTGCT GATAAATCTA 
GTGGAGTATA GTGCTCGGAA TCGGCACTGT CTTGTCAACA TGGAAACATC GTGCTCTTTT 
GATTCTTCCA TCTGTAGTGG AGAAGGGGAT GATAGTTTAA GGATAGGTGG ACAAGTTCAT 
GCTGTCCAGG CTTTAGTGCA GCTATTCCTT GAGCGAGAGC GGGCAGCCCA GCTAGCAGAA 
AGTAAAACAG ATGAGTTGAT CAAAGATGCT CCCACCACTC AGCATGATAA GAGTGGAGAG 
TGGCAAGAAA CAAGTGGAGA AATACAGTGG GTGTCAACTG AAAAGACTGA TGGTACAGAA 
GAGAAACATA AGAAGGAGGA GGAGGATGAA GAACTTGACC TCAATAAAGC CCTTCAGCAT 
GCCGGCAAAC ACATGGAGGA TTGCATTGTG GCCTCCTACA CAGCACTACT TCTTGGGTGT 
CTCTGCCAGG AAAGTCCAAT CAATGTAACC ACTGTGCGGG AATATCTGCC AGAAGGAGAC 
TTTTCAATAA TGACAGAGAT GCTCAAAAAA TTTTTGAGTT TTATGAATCT CACTTGTGCT 
GTTGGAACAA CTGGCCAGAA ATCTATCTCT AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871469.1
CDS	436..3855
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTLYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_017015980.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015980.1

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
TTATATACTG TTGTTCAGCA CGTGAAGCAC TTCAACGATG TTGTAGAATT TGGTGAAAAT 
CAAGAGTTCA CTGATGACAT TGAGTACTTG TTAAGTGGCT TAAAGAGCAC TCAGCCTCTA 
AACACACGTT GCCTTAGTGT TATTAGCTTG GCTACTAAAT GTGCCATGCC CAGTTTTCGA 
ATGCACCTGA GAGCACATGG GATGGTAGCA ATGGTCTTTA AAACCTTGGA TGATTCCCAG 
CACCATCAGA ATCTGTCCCT CTGTACAGCT GCCCTCATGT ATATACTGAG TAGAGATCGT 
TTGAACATGG ATCTTGATAG AGCTAGCTTA GATCTAATGA TTCGACTTTT GGAACTGGAA 
CAAGATGCTT CATCAGCCAA GCTACTGAAT GAAAAAGACA TGAACAAAAT TAAAGAAAAA 
ATCCGAAGGC TCTGTGAAAC TGTACACAAC AAGCATCTTG ATCTAGAAAA TATAACGACT 
GGGCATTTAG CTATGGAGAC ATTATTATCC CTTACTTCTA AACGAGCAGG AGACTGGTTT 
AAAGAAGAAC TCCGGCTTTT GGGTGGTCTG GATCATATTG TAGATAAAGT AAAAGAATGT 
GTGGATCATT TAAGTAGAGA TGAGGATGAA GAGAAACTGG TAGCCTCACT ATGGGGAGCA 
GAGAGATGTT TACGAGTTTT AGAAAGTGTA ACTGTGCATA ATCCCGAAAA TCAAAGCTAC 
TTGATAGCAT ATAAAGATTC CCAACTTATT GTTTCATCAG CTAAAGCATT ACAGCATTGT 
GAAGAACTGA TTCAGCAGTA CAACCGTGCT GAGGACAGCA TATGCTTAGC TGACAGTAAG 
CCTCTGCCTC ACCAGAATGT AACTAACCAT GTAGGCAAAG CAGTGGAGGA CTGCATGAGG 
GCCATCATCG GGGTGTTGCT TAATTTAACT AATGATAATG AGTGGGGCAG CACCAAAACA 
GGAGAGCAGG ACGGTCTCAT AGGCACAGCG CTGAACTGTG TGCTTCAGGT TCCAAAGTAC 
CTACCTCAGG AGCAGAGATT TGATATTCGA GTGCTGGGCT TAGGTCTGCT GATAAATCTA 
GTGGAGTATA GTGCTCGGAA TCGGCACTGT CTTGTCAACA TGGAAACATC GTGCTCTTTT 
GATTCTTCCA TCTGTAGTGG AGAAGGGGAT GATAGTTTAA GGATAGGTGG ACAAGTTCAT 
GCTGTCCAGG CTTTAGTGCA GCTATTCCTT GAGCGAGAGC GGGCAGCCCA GCTAGCAGAA 
AGTAAAACAG ATGAGTTGAT CAAAGATGCT CCCACCACTC AGCATGATAA GAGTGGAGAG 
TGGCAAGAAA CAAGTGGAGA AATACAGTGG GTGTCAACTG AAAAGACTGA TGGTACAGAA 
GAGAAACATA AGAAGGAGGA GGAGGATGAA GAACTTGACC TCAATAAAGC CCTTCAGCAT 
GCCGGCAAAC ACATGGAGGA TTGCATTGTG GCCTCCTACA CAGCACTACT TCTTGGGTGT 
CTCTGCCAGG AAAGTCCAAT CAATGTAACC ACTGTGCGGG AATATCTGCC AGAAGGAGAC 
TTTTCAATAA TGACAGAGAT GCTCAAAAAA TTTTTGAGTT TTATGAATCT CACTTGTGCT 
GTTGGAACAA CTGGCCAGAA ATCTATCTCT AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( NM_015045.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055860.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

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CloneID	OHu06785
Clone ID Related Accession (Same CDS sequence)	NM_015045.3 , NM_015045.5 , NM_015045.4
Accession Version	NM_015045.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB065003.1, AK290138.1, BC140858.1, AL844892.5, BI438983.1 and BC036507.1. On Jan 1, 2016 this sequence version replaced NM_015045.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB065003.1, SRR1660803.171190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055860.1
CDS	443..4015
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_015045.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015045.3

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( NM_015045.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055860.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

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CloneID	OHu06785
Clone ID Related Accession (Same CDS sequence)	NM_015045.3 , NM_015045.5 , NM_015045.4
Accession Version	NM_015045.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731569.18, AB065003.1, AK290138.1, BC140858.1, AL844892.5, BI438983.1 and BC036507.1. On Nov 22, 2018 this sequence version replaced NM_015045.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB065003.1, SRR1660803.171190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055860.1
CDS	451..4023
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_015045.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015045.4

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAAAAC TTGGATTTTA CAGAGGACTT GCCTGGTGTG 
CCTGAAAGTG TGAAGAAGCC CATAAATAAA CAAGGAGATA AATCAAAGGA AAATACCAGA 
AAGATTTTTA GTGGCCCCAA ACGGTCACCC ACAAAAGCTG TATATAATGC CAGACATTGG 
AATCATCCAG ATTCAGAAGA ACTGCCTGGG CCACCAGTAG TAAAACCTCA GAGTGTCACA 
GTGAGGCTGT CTTCAAAGGA ACCAAATCAA AAAGATGATG GAGTTTTTAA GGCTCCTGCA 
CCACCATCCA AAGTGATAAA AACTGTGACA ATACCTACTC AGCCCTACCA AGATATAGTT 
ACTGCACTGA AATGCAGACG AGAAGACAAA GAATTATATA CTGTTGTTCA GCACGTGAAG 
CACTTCAACG ATGTTGTAGA ATTTGGTGAA AATCAAGAGT TCACTGATGA CATTGAGTAC 
TTGTTAAGTG GCTTAAAGAG CACTCAGCCT CTAAACACAC GTTGCCTTAG TGTTATTAGC 
TTGGCTACTA AATGTGCCAT GCCCAGTTTT CGAATGCACC TGAGAGCACA TGGGATGGTA 
GCAATGGTCT TTAAAACCTT GGATGATTCC CAGCACCATC AGAATCTGTC CCTCTGTACA 
GCTGCCCTCA TGTATATACT GAGTAGAGAT CGTTTGAACA TGGATCTTGA TAGAGCTAGC 
TTAGATCTAA TGATTCGACT TTTGGAACTG GAACAAGATG CTTCATCAGC CAAGCTACTG 
AATGAAAAAG ACATGAACAA AATTAAAGAA AAAATCCGAA GGCTCTGTGA AACTGTACAC 
AACAAGCATC TTGATCTAGA AAATATAACG ACTGGGCATT TAGCTATGGA GACATTATTA 
TCCCTTACTT CTAAACGAGC AGGAGACTGG TTTAAAGAAG AACTCCGGCT TTTGGGTGGT 
CTGGATCATA TTGTAGATAA AGTAAAAGAA TGTGTGGATC ATTTAAGTAG AGATGAGGAT 
GAAGAGAAAC TGGTAGCCTC ACTATGGGGA GCAGAGAGAT GTTTACGAGT TTTAGAAAGT 
GTAACTGTGC ATAATCCCGA AAATCAAAGC TACTTGATAG CATATAAAGA TTCCCAACTT 
ATTGTTTCAT CAGCTAAAGC ATTACAGCAT TGTGAAGAAC TGATTCAGCA GTACAACCGT 
GCTGAGGACA GCATATGCTT AGCTGACAGT AAGCCTCTGC CTCACCAGAA TGTAACTAAC 
CATGTAGGCA AAGCAGTGGA GGACTGCATG AGGGCCATCA TCGGGGTGTT GCTTAATTTA 
ACTAATGATA ATGAGTGGGG CAGCACCAAA ACAGGAGAGC AGGACGGTCT CATAGGCACA 
GCGCTGAACT GTGTGCTTCA GGTTCCAAAG TACCTACCTC AGGAGCAGAG ATTTGATATT 
CGAGTGCTGG GCTTAGGTCT GCTGATAAAT CTAGTGGAGT ATAGTGCTCG GAATCGGCAC 
TGTCTTGTCA ACATGGAAAC ATCGTGCTCT TTTGATTCTT CCATCTGTAG TGGAGAAGGG 
GATGATAGTT TAAGGATAGG TGGACAAGTT CATGCTGTCC AGGCTTTAGT GCAGCTATTC 
CTTGAGCGAG AGCGGGCAGC CCAGCTAGCA GAAAGTAAAA CAGATGAGTT GATCAAAGAT 
GCTCCCACCA CTCAGCATGA TAAGAGTGGA GAGTGGCAAG AAACAAGTGG AGAAATACAG 
TGGGTGTCAA CTGAAAAGAC TGATGGTACA GAAGAGAAAC ATAAGAAGGA GGAGGAGGAT 
GAAGAACTTG ACCTCAATAA AGCCCTTCAG CATGCCGGCA AACACATGGA GGATTGCATT 
GTGGCCTCCT ACACAGCACT ACTTCTTGGG TGTCTCTGCC AGGAAAGTCC AATCAATGTA 
ACCACTGTGC GGGAATATCT GCCAGAAGGA GACTTTTCAA TAATGACAGA GATGCTCAAA 
AAATTTTTGA GTTTTATGAA TCTCACTTGT GCTGTTGGAA CAACTGGCCA GAAATCTATC 
TCTAGAGTGA TTGAATATTT GGAACATTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( NM_001318328.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305257.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 2, mRNA.

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CloneID	OHu44161
Clone ID Related Accession (Same CDS sequence)	NM_001318328.1
Accession Version	NM_001318328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB065003.1, AK290138.1, BC140858.1, AL844892.5, BI438983.1 and BC036507.1. On Jan 1, 2016 this sequence version replaced XM_006717729.2. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.31477.1, BC140858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAGGAC TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG 
CCCATAAATA AACAAGGAGA TAAATCAAAG GAAAATACCA GAAAGATTTT TAGTGGCCCC 
AAACGGTCAC CCACAAAAGC TGTATATAAT GCCAGACATT GGAATCATCC AGATTCAGAA 
GAACTGCCTG GGCCACCAGT AGTAAAACCT CAGAGTGTCA CAGTGAGGCT GTCTTCAAAG 
GAACCAAATC AAAAAGATGA TGGAGTTTTT AAGGCTCCTG CACCACCATC CAAAGTGATA 
AAAACTGTGA CAATACCTAC TCAGCCCTAC CAAGATATAG TTACTGCACT GAAATGCAGA 
CGAGAAGACA AAGAATTATA TACTGTTGTT CAGCACGTGA AGCACTTCAA CGATGTTGTA 
GAATTTGGTG AAAATCAAGA GTTCACTGAT GACATTGAGT ACTTGTTAAG TGGCTTAAAG 
AGCACTCAGC CTCTAAACAC ACGTTGCCTT AGTGTTATTA GCTTGGCTAC TAAATGTGCC 
ATGCCCAGTT TTCGAATGCA CCTGAGAGCA CATGGGATGG TAGCAATGGT CTTTAAAACC 
TTGGATGATT CCCAGCACCA TCAGAATCTG TCCCTCTGTA CAGCTGCCCT CATGTATATA 
CTGAGTAGAG ATCGTTTGAA CATGGATCTT GATAGAGCTA GCTTAGATCT AATGATTCGA 
CTTTTGGAAC TGGAACAAGA TGCTTCATCA GCCAAGCTAC TGAATGAAAA AGACATGAAC 
AAAATTAAAG AAAAAATCCG AAGGCTCTGT GAAACTGTAC ACAACAAGCA TCTTGATCTA 
GAAAATATAA CGACTGGGCA TTTAGCTATG GAGACATTAT TATCCCTTAC TTCTAAACGA 
GCAGGAGACT GGTTTAAAGA AGAACTCCGG CTTTTGGGTG GTCTGGATCA TATTGTAGAT 
AAAGTAAAAG AATGTGTGGA TCATTTAAGT AGAGATGAGG ATGAAGAGAA ACTGGTAGCC 
TCACTATGGG GAGCAGAGAG ATGTTTACGA GTTTTAGAAA GTGTAACTGT GCATAATCCC 
GAAAATCAAA GCTACTTGAT AGCATATAAA GATTCCCAAC TTATTGTTTC ATCAGCTAAA 
GCATTACAGC ATTGTGAAGA ACTGATTCAG CAGTACAACC GTGCTGAGGA CAGCATATGC 
TTAGCTGACA GTAAGCCTCT GCCTCACCAG AATGTAACTA ACCATGTAGG CAAAGCAGTG 
GAGGACTGCA TGAGGGCCAT CATCGGGGTG TTGCTTAATT TAACTAATGA TAATGAGTGG 
GGCAGCACCA AAACAGGAGA GCAGGACGGT CTCATAGGCA CAGCGCTGAA CTGTGTGCTT 
CAGGTTCCAA AGTACCTACC TCAGGAGCAG AGATTTGATA TTCGAGTGCT GGGCTTAGGT 
CTGCTGATAA ATCTAGTGGA GTATAGTGCT CGGAATCGGC ACTGTCTTGT CAACATGGAA 
ACATCGTGCT CTTTTGATTC TTCCATCTGT AGTGGAGAAG GGGATGATAG TTTAAGGATA 
GGTGGACAAG TTCATGCTGT CCAGGCTTTA GTGCAGCTAT TCCTTGAGCG AGAGCGGGCA 
GCCCAGCTAG CAGAAAGTAA AACAGATGAG TTGATCAAAG ATGCTCCCAC CACTCAGCAT 
GATAAGAGTG GAGAGTGGCA AGAAACAAGT GGAGAAATAC AGTGGGTGTC AACTGAAAAG 
ACTGATGGTA CAGAAGAGAA ACATAAGAAG GAGGAGGAGG ATGAAGAACT TGACCTCAAT 
AAAGCCCTTC AGCATGCCGG CAAACACATG GAGGATTGCA TTGTGGCCTC CTACACAGCA 
CTACTTCTTG GGTGTCTCTG CCAGGAAAGT CCAATCAATG TAACCACTGT GCGGGAATAT 
CTGCCAGAAG GAGACTTTTC AATAATGACA GAGATGCTCA AAAAATTTTT GAGTTTTATG 
AATCTCACTT GTGCTGTTGG AACAACTGGC CAGAAATCTA TCTCTAGAGT GATTGAATAT 
TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305257.1
CDS	443..3997
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_001318328.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318328.1

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGAGGAC TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG 
CCCATAAATA AACAAGGAGA TAAATCAAAG GAAAATACCA GAAAGATTTT TAGTGGCCCC 
AAACGGTCAC CCACAAAAGC TGTATATAAT GCCAGACATT GGAATCATCC AGATTCAGAA 
GAACTGCCTG GGCCACCAGT AGTAAAACCT CAGAGTGTCA CAGTGAGGCT GTCTTCAAAG 
GAACCAAATC AAAAAGATGA TGGAGTTTTT AAGGCTCCTG CACCACCATC CAAAGTGATA 
AAAACTGTGA CAATACCTAC TCAGCCCTAC CAAGATATAG TTACTGCACT GAAATGCAGA 
CGAGAAGACA AAGAATTATA TACTGTTGTT CAGCACGTGA AGCACTTCAA CGATGTTGTA 
GAATTTGGTG AAAATCAAGA GTTCACTGAT GACATTGAGT ACTTGTTAAG TGGCTTAAAG 
AGCACTCAGC CTCTAAACAC ACGTTGCCTT AGTGTTATTA GCTTGGCTAC TAAATGTGCC 
ATGCCCAGTT TTCGAATGCA CCTGAGAGCA CATGGGATGG TAGCAATGGT CTTTAAAACC 
TTGGATGATT CCCAGCACCA TCAGAATCTG TCCCTCTGTA CAGCTGCCCT CATGTATATA 
CTGAGTAGAG ATCGTTTGAA CATGGATCTT GATAGAGCTA GCTTAGATCT AATGATTCGA 
CTTTTGGAAC TGGAACAAGA TGCTTCATCA GCCAAGCTAC TGAATGAAAA AGACATGAAC 
AAAATTAAAG AAAAAATCCG AAGGCTCTGT GAAACTGTAC ACAACAAGCA TCTTGATCTA 
GAAAATATAA CGACTGGGCA TTTAGCTATG GAGACATTAT TATCCCTTAC TTCTAAACGA 
GCAGGAGACT GGTTTAAAGA AGAACTCCGG CTTTTGGGTG GTCTGGATCA TATTGTAGAT 
AAAGTAAAAG AATGTGTGGA TCATTTAAGT AGAGATGAGG ATGAAGAGAA ACTGGTAGCC 
TCACTATGGG GAGCAGAGAG ATGTTTACGA GTTTTAGAAA GTGTAACTGT GCATAATCCC 
GAAAATCAAA GCTACTTGAT AGCATATAAA GATTCCCAAC TTATTGTTTC ATCAGCTAAA 
GCATTACAGC ATTGTGAAGA ACTGATTCAG CAGTACAACC GTGCTGAGGA CAGCATATGC 
TTAGCTGACA GTAAGCCTCT GCCTCACCAG AATGTAACTA ACCATGTAGG CAAAGCAGTG 
GAGGACTGCA TGAGGGCCAT CATCGGGGTG TTGCTTAATT TAACTAATGA TAATGAGTGG 
GGCAGCACCA AAACAGGAGA GCAGGACGGT CTCATAGGCA CAGCGCTGAA CTGTGTGCTT 
CAGGTTCCAA AGTACCTACC TCAGGAGCAG AGATTTGATA TTCGAGTGCT GGGCTTAGGT 
CTGCTGATAA ATCTAGTGGA GTATAGTGCT CGGAATCGGC ACTGTCTTGT CAACATGGAA 
ACATCGTGCT CTTTTGATTC TTCCATCTGT AGTGGAGAAG GGGATGATAG TTTAAGGATA 
GGTGGACAAG TTCATGCTGT CCAGGCTTTA GTGCAGCTAT TCCTTGAGCG AGAGCGGGCA 
GCCCAGCTAG CAGAAAGTAA AACAGATGAG TTGATCAAAG ATGCTCCCAC CACTCAGCAT 
GATAAGAGTG GAGAGTGGCA AGAAACAAGT GGAGAAATAC AGTGGGTGTC AACTGAAAAG 
ACTGATGGTA CAGAAGAGAA ACATAAGAAG GAGGAGGAGG ATGAAGAACT TGACCTCAAT 
AAAGCCCTTC AGCATGCCGG CAAACACATG GAGGATTGCA TTGTGGCCTC CTACACAGCA 
CTACTTCTTG GGTGTCTCTG CCAGGAAAGT CCAATCAATG TAACCACTGT GCGGGAATAT 
CTGCCAGAAG GAGACTTTTC AATAATGACA GAGATGCTCA AAAAATTTTT GAGTTTTATG 
AATCTCACTT GTGCTGTTGG AACAACTGGC CAGAAATCTA TCTCTAGAGT GATTGAATAT 
TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_017015979.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871468.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

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CloneID	OHu65376
Clone ID Related Accession (Same CDS sequence)	XM_017015979.1
Accession Version	XM_017015979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGTTTTG ATTACCATTT CAGAAAACTT GGATTTTACA 
GAGGACTTGC CTGGTGTGCC TGAAAGTGTG AAGAAGCCCA TAAATAAACA AGGAGATAAA 
TCAAAGGAAA ATACCAGAAA GATTTTTAGT GGCCCCAAAC GGTCACCCAC AAAAGCTGTA 
TATAATGCCA GACATTGGAA TCATCCAGAT TCAGAAGAAC TGCCTGGGCC ACCAGTAGTA 
AAACCTCAGA GTGTCACATT ATATACTGTT GTTCAGCACG TGAAGCACTT CAACGATGTT 
GTAGAATTTG GTGAAAATCA AGAGTTCACT GATGACATTG AGTACTTGTT AAGTGGCTTA 
AAGAGCACTC AGCCTCTAAA CACACGTTGC CTTAGTGTTA TTAGCTTGGC TACTAAATGT 
GCCATGCCCA GTTTTCGAAT GCACCTGAGA GCACATGGGA TGGTAGCAAT GGTCTTTAAA 
ACCTTGGATG ATTCCCAGCA CCATCAGAAT CTGTCCCTCT GTACAGCTGC CCTCATGTAT 
ATACTGAGTA GAGATCGTTT GAACATGGAT CTTGATAGAG CTAGCTTAGA TCTAATGATT 
CGACTTTTGG AACTGGAACA AGATGCTTCA TCAGCCAAGC TACTGAATGA AAAAGACATG 
AACAAAATTA AAGAAAAAAT CCGAAGGCTC TGTGAAACTG TACACAACAA GCATCTTGAT 
CTAGAAAATA TAACGACTGG GCATTTAGCT ATGGAGACAT TATTATCCCT TACTTCTAAA 
CGAGCAGGAG ACTGGTTTAA AGAAGAACTC CGGCTTTTGG GTGGTCTGGA TCATATTGTA 
GATAAAGTAA AAGAATGTGT GGATCATTTA AGTAGAGATG AGGATGAAGA GAAACTGGTA 
GCCTCACTAT GGGGAGCAGA GAGATGTTTA CGAGTTTTAG AAAGTGTAAC TGTGCATAAT 
CCCGAAAATC AAAGCTACTT GATAGCATAT AAAGATTCCC AACTTATTGT TTCATCAGCT 
AAAGCATTAC AGCATTGTGA AGAACTGATT CAGCAGTACA ACCGTGCTGA GGACAGCATA 
TGCTTAGCTG ACAGTAAGCC TCTGCCTCAC CAGAATGTAA CTAACCATGT AGGCAAAGCA 
GTGGAGGACT GCATGAGGGC CATCATCGGG GTGTTGCTTA ATTTAACTAA TGATAATGAG 
TGGGGCAGCA CCAAAACAGG AGAGCAGGAC GGTCTCATAG GCACAGCGCT GAACTGTGTG 
CTTCAGGTTC CAAAGTACCT ACCTCAGGAG CAGAGATTTG ATATTCGAGT GCTGGGCTTA 
GGTCTGCTGA TAAATCTAGT GGAGTATAGT GCTCGGAATC GGCACTGTCT TGTCAACATG 
GAAACATCGT GCTCTTTTGA TTCTTCCATC TGTAGTGGAG AAGGGGATGA TAGTTTAAGG 
ATAGGTGGAC AAGTTCATGC TGTCCAGGCT TTAGTGCAGC TATTCCTTGA GCGAGAGCGG 
GCAGCCCAGC TAGCAGAAAG TAAAACAGAT GAGTTGATCA AAGATGCTCC CACCACTCAG 
CATGATAAGA GTGGAGAGTG GCAAGAAACA AGTGGAGAAA TACAGTGGGT GTCAACTGAA 
AAGACTGATG GTACAGAAGA GAAACATAAG AAGGAGGAGG AGGATGAAGA ACTTGACCTC 
AATAAAGCCC TTCAGCATGC CGGCAAACAC ATGGAGGATT GCATTGTGGC CTCCTACACA 
GCACTACTTC TTGGGTGTCT CTGCCAGGAA AGTCCAATCA ATGTAACCAC TGTGCGGGAA 
TATCTGCCAG AAGGAGACTT TTCAATAATG ACAGAGATGC TCAAAAAATT TTTGAGTTTT 
ATGAATCTCA CTTGTGCTGT TGGAACAACT GGCCAGAAAT CTATCTCTAG AGTGATTGAA 
TATTTGGAAC ATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871468.1
CDS	475..3912
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAVLITISENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTLYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_017015979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015979.1

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGTTTTG ATTACCATTT CAGAAAACTT GGATTTTACA 
GAGGACTTGC CTGGTGTGCC TGAAAGTGTG AAGAAGCCCA TAAATAAACA AGGAGATAAA 
TCAAAGGAAA ATACCAGAAA GATTTTTAGT GGCCCCAAAC GGTCACCCAC AAAAGCTGTA 
TATAATGCCA GACATTGGAA TCATCCAGAT TCAGAAGAAC TGCCTGGGCC ACCAGTAGTA 
AAACCTCAGA GTGTCACATT ATATACTGTT GTTCAGCACG TGAAGCACTT CAACGATGTT 
GTAGAATTTG GTGAAAATCA AGAGTTCACT GATGACATTG AGTACTTGTT AAGTGGCTTA 
AAGAGCACTC AGCCTCTAAA CACACGTTGC CTTAGTGTTA TTAGCTTGGC TACTAAATGT 
GCCATGCCCA GTTTTCGAAT GCACCTGAGA GCACATGGGA TGGTAGCAAT GGTCTTTAAA 
ACCTTGGATG ATTCCCAGCA CCATCAGAAT CTGTCCCTCT GTACAGCTGC CCTCATGTAT 
ATACTGAGTA GAGATCGTTT GAACATGGAT CTTGATAGAG CTAGCTTAGA TCTAATGATT 
CGACTTTTGG AACTGGAACA AGATGCTTCA TCAGCCAAGC TACTGAATGA AAAAGACATG 
AACAAAATTA AAGAAAAAAT CCGAAGGCTC TGTGAAACTG TACACAACAA GCATCTTGAT 
CTAGAAAATA TAACGACTGG GCATTTAGCT ATGGAGACAT TATTATCCCT TACTTCTAAA 
CGAGCAGGAG ACTGGTTTAA AGAAGAACTC CGGCTTTTGG GTGGTCTGGA TCATATTGTA 
GATAAAGTAA AAGAATGTGT GGATCATTTA AGTAGAGATG AGGATGAAGA GAAACTGGTA 
GCCTCACTAT GGGGAGCAGA GAGATGTTTA CGAGTTTTAG AAAGTGTAAC TGTGCATAAT 
CCCGAAAATC AAAGCTACTT GATAGCATAT AAAGATTCCC AACTTATTGT TTCATCAGCT 
AAAGCATTAC AGCATTGTGA AGAACTGATT CAGCAGTACA ACCGTGCTGA GGACAGCATA 
TGCTTAGCTG ACAGTAAGCC TCTGCCTCAC CAGAATGTAA CTAACCATGT AGGCAAAGCA 
GTGGAGGACT GCATGAGGGC CATCATCGGG GTGTTGCTTA ATTTAACTAA TGATAATGAG 
TGGGGCAGCA CCAAAACAGG AGAGCAGGAC GGTCTCATAG GCACAGCGCT GAACTGTGTG 
CTTCAGGTTC CAAAGTACCT ACCTCAGGAG CAGAGATTTG ATATTCGAGT GCTGGGCTTA 
GGTCTGCTGA TAAATCTAGT GGAGTATAGT GCTCGGAATC GGCACTGTCT TGTCAACATG 
GAAACATCGT GCTCTTTTGA TTCTTCCATC TGTAGTGGAG AAGGGGATGA TAGTTTAAGG 
ATAGGTGGAC AAGTTCATGC TGTCCAGGCT TTAGTGCAGC TATTCCTTGA GCGAGAGCGG 
GCAGCCCAGC TAGCAGAAAG TAAAACAGAT GAGTTGATCA AAGATGCTCC CACCACTCAG 
CATGATAAGA GTGGAGAGTG GCAAGAAACA AGTGGAGAAA TACAGTGGGT GTCAACTGAA 
AAGACTGATG GTACAGAAGA GAAACATAAG AAGGAGGAGG AGGATGAAGA ACTTGACCTC 
AATAAAGCCC TTCAGCATGC CGGCAAACAC ATGGAGGATT GCATTGTGGC CTCCTACACA 
GCACTACTTC TTGGGTGTCT CTGCCAGGAA AGTCCAATCA ATGTAACCAC TGTGCGGGAA 
TATCTGCCAG AAGGAGACTT TTCAATAATG ACAGAGATGC TCAAAAAATT TTTGAGTTTT 
ATGAATCTCA CTTGTGCTGT TGGAACAACT GGCCAGAAAT CTATCTCTAG AGTGATTGAA 
TATTTGGAAC ATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_011539548.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537850.1 Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

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CloneID	OHu65377
Clone ID Related Accession (Same CDS sequence)	XM_011539548.1
Accession Version	XM_011539548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	wings apart-like protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGTTTTG ATTACCATTT CAGAAAACTT GGATTTTACA 
GAGGACTTGC CTGGTGTGCC TGAAAGTGTG AAGAAGCCCA TAAATAAACA AGGAGATAAA 
TCAAAGGAAA ATACCAGAAA GATTTTTAGT GGCCCCAAAC GGTCACCCAC AAAAGCTGTA 
TATAATGCCA GACATTGGAA TCATCCAGAT TCAGAAGAAC TGCCTGGGCC ACCAGTAGTA 
AAACCTCAGA GTGTCACAGT GAGGCTGTCT TCAAAGGAAC CAAATCAAAA AGATGATGGA 
GTTTTTAAGG CTCCTGCACC ACCATCCAAA GTGATAAAAA CTGTGACAAT ACCTACTCAG 
CCCTACCAAG ATATAGTTAC TGCACTGAAA TGCAGACGAG AAGACAAAGA ATTATATACT 
GTTGTTCAGC ACGTGAAGCA CTTCAACGAT GTTGTAGAAT TTGGTGAAAA TCAAGAGTTC 
ACTGATGACA TTGAGTACTT GTTAAGTGGC TTAAAGAGCA CTCAGCCTCT AAACACACGT 
TGCCTTAGTG TTATTAGCTT GGCTACTAAA TGTGCCATGC CCAGTTTTCG AATGCACCTG 
AGAGCACATG GGATGGTAGC AATGGTCTTT AAAACCTTGG ATGATTCCCA GCACCATCAG 
AATCTGTCCC TCTGTACAGC TGCCCTCATG TATATACTGA GTAGAGATCG TTTGAACATG 
GATCTTGATA GAGCTAGCTT AGATCTAATG ATTCGACTTT TGGAACTGGA ACAAGATGCT 
TCATCAGCCA AGCTACTGAA TGAAAAAGAC ATGAACAAAA TTAAAGAAAA AATCCGAAGG 
CTCTGTGAAA CTGTACACAA CAAGCATCTT GATCTAGAAA ATATAACGAC TGGGCATTTA 
GCTATGGAGA CATTATTATC CCTTACTTCT AAACGAGCAG GAGACTGGTT TAAAGAAGAA 
CTCCGGCTTT TGGGTGGTCT GGATCATATT GTAGATAAAG TAAAAGAATG TGTGGATCAT 
TTAAGTAGAG ATGAGGATGA AGAGAAACTG GTAGCCTCAC TATGGGGAGC AGAGAGATGT 
TTACGAGTTT TAGAAAGTGT AACTGTGCAT AATCCCGAAA ATCAAAGCTA CTTGATAGCA 
TATAAAGATT CCCAACTTAT TGTTTCATCA GCTAAAGCAT TACAGCATTG TGAAGAACTG 
ATTCAGCAGT ACAACCGTGC TGAGGACAGC ATATGCTTAG CTGACAGTAA GCCTCTGCCT 
CACCAGAATG TAACTAACCA TGTAGGCAAA GCAGTGGAGG ACTGCATGAG GGCCATCATC 
GGGGTGTTGC TTAATTTAAC TAATGATAAT GAGTGGGGCA GCACCAAAAC AGGAGAGCAG 
GACGGTCTCA TAGGCACAGC GCTGAACTGT GTGCTTCAGG TTCCAAAGTA CCTACCTCAG 
GAGCAGAGAT TTGATATTCG AGTGCTGGGC TTAGGTCTGC TGATAAATCT AGTGGAGTAT 
AGTGCTCGGA ATCGGCACTG TCTTGTCAAC ATGGAAACAT CGTGCTCTTT TGATTCTTCC 
ATCTGTAGTG GAGAAGGGGA TGATAGTTTA AGGATAGGTG GACAAGTTCA TGCTGTCCAG 
GCTTTAGTGC AGCTATTCCT TGAGCGAGAG CGGGCAGCCC AGCTAGCAGA AAGTAAAACA 
GATGAGTTGA TCAAAGATGC TCCCACCACT CAGCATGATA AGAGTGGAGA GTGGCAAGAA 
ACAAGTGGAG AAATACAGTG GGTGTCAACT GAAAAGACTG ATGGTACAGA AGAGAAACAT 
AAGAAGGAGG AGGAGGATGA AGAACTTGAC CTCAATAAAG CCCTTCAGCA TGCCGGCAAA 
CACATGGAGG ATTGCATTGT GGCCTCCTAC ACAGCACTAC TTCTTGGGTG TCTCTGCCAG 
GAAAGTCCAA TCAATGTAAC CACTGTGCGG GAATATCTGC CAGAAGGAGA CTTTTCAATA 
ATGACAGAGA TGCTCAAAAA ATTTTTGAGT TTTATGAATC TCACTTGTGC TGTTGGAACA 
ACTGGCCAGA AATCTATCTC TAGAGTGATT GAATATTTGG AACATTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537850.1
CDS	475..4065
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAVLITISENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_011539548.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539548.1

ORF Insert Sequence:

ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGTG GAAATGGCAG TTCAAAATTC 
GATGAAGTCT TTTCCAACAA ACGGACTACC CTTAGCACAA AATGGGGAGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AATTCCGAAG 
AAACCTAAAG TGGAAGAAGA AAGTACTGGA GATCCTTTTG GATTTGATAG TGATGATGAG 
TCTCTACCAG TTTCTTCAAA GAATTTAGCC CAGGTTAAGT GTTCCTCTTA TTCAGAATCT 
AGTGAAGCTG CTCAGTTGGA AGAGGTCACT TCAGTACTTG AAGCTAATAG CAAAATTAGT 
CATGTGGTCG TTGAAGACAC TGTCGTTTCT GATAAATGCT TCCCTTTGGA GGACACTTTA 
CTTGGGAAAG AAAAGAGCAC AAACCGAATT GTAGAAGATG ATGCAAGCAT AAGTAGCTGT 
AATAAATTAA TAACTTCAGA TAAAGTGGAG AATTTTCATG AAGAACATGA AAAGAATAGT 
CACCATATTC ACAAAAATGC TGATGACAGT ACTAAGAAAC CCAATGCAGA AACTACAGTG 
GCTTCTGAAA TCAAGGAAAC AAATGATACT TGGAACTCCC AGTTTGGGAA AAGGCCAGAA 
TCACCATCAG AAATATCTCC AATCAAGGGA TCTGTTAGAA CTGGTTTGTT TGAATGGGAT 
AATGATTTTG AAGATATCAG ATCAGAAGAC TGTATTTTAA GTTTGGATAG TGATCCCCTT 
TTGGAGATGA AGGATGACGA TTTTAAAAAT CGATTGGAAA ATCTGAATGA AGCCATTGAG 
GAAGATATTG TACAAAGTGT TCTTAGGCCA ACCAACTGTA GGACGTACTG TAGGGCCAAT 
AAAACGAAAT CCTCCCAAGG AGCATCAAAT TTTGATAAGC TGATGGACGG CACCAGTCAG 
GCCTTAGCCA AAGCAAACAG TGAATCGAGT AAAGATGGCC TGAATCAGGC AAAGAAAGGG 
GGTGTAAGTT GTGGGACCAG TTTTAGAGGG ACAGTTGGAC GGACTAGAGA TTACACTGTT 
TTACATCCAT CTTGCTTGTC AGTTTGTAAT GTTACCATAC AGGATACTAT GGAACGCAGC 
ATGGATGAGT TCACTGCATC CACTCCTGCA GATTTGGGAG AAGCTGGTCG TCTCAGAAAA 
AAGGCAGATA TTGCAACTTC TAAGACTACT ACTAGATTTC GACCTAGTAA TACTAAATCC 
AAAAAGGATG TTAAACTTGA ATTTTTTGGT TTTGAAGATC ATGAGACAGG AGGTGATGAA 
GGAGGTTCTG GAAGTTCTAA TTACAAAATT AAGTATTTTG GCTTTGATGA TCTCAGTGAA 
AGCGAAGATG ATGAAGATGA TGACTGTCAA GTAGAAAGAA AGACAAGCAA AAAAAGAACT 
AAAACAGCTC CATCACCCTC CTTGCAGCCT CCCCCAGAAA GCAATGATAA TTCCCAGGAC 
AGTCAGTCTG GTACTAACAA TGCAGTTTTG ATTACCATTT CAGAAAACTT GGATTTTACA 
GAGGACTTGC CTGGTGTGCC TGAAAGTGTG AAGAAGCCCA TAAATAAACA AGGAGATAAA 
TCAAAGGAAA ATACCAGAAA GATTTTTAGT GGCCCCAAAC GGTCACCCAC AAAAGCTGTA 
TATAATGCCA GACATTGGAA TCATCCAGAT TCAGAAGAAC TGCCTGGGCC ACCAGTAGTA 
AAACCTCAGA GTGTCACAGT GAGGCTGTCT TCAAAGGAAC CAAATCAAAA AGATGATGGA 
GTTTTTAAGG CTCCTGCACC ACCATCCAAA GTGATAAAAA CTGTGACAAT ACCTACTCAG 
CCCTACCAAG ATATAGTTAC TGCACTGAAA TGCAGACGAG AAGACAAAGA ATTATATACT 
GTTGTTCAGC ACGTGAAGCA CTTCAACGAT GTTGTAGAAT TTGGTGAAAA TCAAGAGTTC 
ACTGATGACA TTGAGTACTT GTTAAGTGGC TTAAAGAGCA CTCAGCCTCT AAACACACGT 
TGCCTTAGTG TTATTAGCTT GGCTACTAAA TGTGCCATGC CCAGTTTTCG AATGCACCTG 
AGAGCACATG GGATGGTAGC AATGGTCTTT AAAACCTTGG ATGATTCCCA GCACCATCAG 
AATCTGTCCC TCTGTACAGC TGCCCTCATG TATATACTGA GTAGAGATCG TTTGAACATG 
GATCTTGATA GAGCTAGCTT AGATCTAATG ATTCGACTTT TGGAACTGGA ACAAGATGCT 
TCATCAGCCA AGCTACTGAA TGAAAAAGAC ATGAACAAAA TTAAAGAAAA AATCCGAAGG 
CTCTGTGAAA CTGTACACAA CAAGCATCTT GATCTAGAAA ATATAACGAC TGGGCATTTA 
GCTATGGAGA CATTATTATC CCTTACTTCT AAACGAGCAG GAGACTGGTT TAAAGAAGAA 
CTCCGGCTTT TGGGTGGTCT GGATCATATT GTAGATAAAG TAAAAGAATG TGTGGATCAT 
TTAAGTAGAG ATGAGGATGA AGAGAAACTG GTAGCCTCAC TATGGGGAGC AGAGAGATGT 
TTACGAGTTT TAGAAAGTGT AACTGTGCAT AATCCCGAAA ATCAAAGCTA CTTGATAGCA 
TATAAAGATT CCCAACTTAT TGTTTCATCA GCTAAAGCAT TACAGCATTG TGAAGAACTG 
ATTCAGCAGT ACAACCGTGC TGAGGACAGC ATATGCTTAG CTGACAGTAA GCCTCTGCCT 
CACCAGAATG TAACTAACCA TGTAGGCAAA GCAGTGGAGG ACTGCATGAG GGCCATCATC 
GGGGTGTTGC TTAATTTAAC TAATGATAAT GAGTGGGGCA GCACCAAAAC AGGAGAGCAG 
GACGGTCTCA TAGGCACAGC GCTGAACTGT GTGCTTCAGG TTCCAAAGTA CCTACCTCAG 
GAGCAGAGAT TTGATATTCG AGTGCTGGGC TTAGGTCTGC TGATAAATCT AGTGGAGTAT 
AGTGCTCGGA ATCGGCACTG TCTTGTCAAC ATGGAAACAT CGTGCTCTTT TGATTCTTCC 
ATCTGTAGTG GAGAAGGGGA TGATAGTTTA AGGATAGGTG GACAAGTTCA TGCTGTCCAG 
GCTTTAGTGC AGCTATTCCT TGAGCGAGAG CGGGCAGCCC AGCTAGCAGA AAGTAAAACA 
GATGAGTTGA TCAAAGATGC TCCCACCACT CAGCATGATA AGAGTGGAGA GTGGCAAGAA 
ACAAGTGGAG AAATACAGTG GGTGTCAACT GAAAAGACTG ATGGTACAGA AGAGAAACAT 
AAGAAGGAGG AGGAGGATGA AGAACTTGAC CTCAATAAAG CCCTTCAGCA TGCCGGCAAA 
CACATGGAGG ATTGCATTGT GGCCTCCTAC ACAGCACTAC TTCTTGGGTG TCTCTGCCAG 
GAAAGTCCAA TCAATGTAAC CACTGTGCGG GAATATCTGC CAGAAGGAGA CTTTTCAATA 
ATGACAGAGA TGCTCAAAAA ATTTTTGAGT TTTATGAATC TCACTTGTGC TGTTGGAACA 
ACTGGCCAGA AATCTATCTC TAGAGTGATT GAATATTTGG AACATTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion.
EMBO reports19(1)43-56(2018 Jan)
Liang C,Chen Q,Yi Q,Zhang M,Yan H,Zhang B,Zhou L,Zhang Z,Qi F,Ye S,Wang F

Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.
The EMBO journal36(24)3573-3599(2017 12)
Wutz G,V?rnai C,Nagasaka K,Cisneros DA,Stocsits RR,Tang W,Schoenfelder S,Jessberger G,Muhar M,Hossain MJ,Walther N,Koch B,Kueblbeck M,Ellenberg J,Zuber J,Fraser P,Peters JM

The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension.
Cell169(4)693-707.e14(2017 May)
Haarhuis JHI,van der Weide RH,Blomen VA,Y??ez-Cuna JO,Amendola M,van Ruiten MS,Krijger PHL,Teunissen H,Medema RH,van Steensel B,Brummelkamp TR,de Wit E,Rowland BD

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WAPL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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