RPIA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPIA
Entrez Gene ID	22934
Full Name	ribose 5-phosphate isomerase A
Synonyms	RPI,RPIAD
General protein information	Preferred Names ribose 5-phosphate isomerase A Names ribose-5-phosphate isomerase phosphoriboisomerase ribose 5-phosphate epimerase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p11.2
Summary	The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18. [provided by RefSeq, Mar 2010].
Disorder MIM:	180430
Disorder Html:	?Ribose 5-phosphate isomerase deficiency, 608611 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_144563.2	NP_653164.2	ribose-5-phosphate isomerase
NM_144563.3	NP_653164.2	ribose-5-phosphate isomerase

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPIA	NP_653164.2
Danio rerio (zebrafish)	rpia	NP_001007290.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011457	XP_554756.3
Arabidopsis thaliana (thale cress)	RSW10	NP_177266.1
Xenopus tropicalis (tropical clawed frog)	rpia	XP_004919660.1
	RPIA	XP_001094618.2
Mus musculus (house mouse)	Rpia	NP_033101.2
Gallus gallus (chicken)	RPIA	NP_001026341.1
Kluyveromyces lactis	KLLA0C13541g	XP_452804.1
Schizosaccharomyces pombe (fission yeast)	SPAC144.12	NP_594673.1
Arabidopsis thaliana (thale cress)	RPI2	NP_178238.1
Bos taurus (cattle)	RPIA	NP_001030510.2
Drosophila melanogaster (fruit fly)	Rpi	NP_001246480.1
Pan troglodytes (chimpanzee)	RPIA	XP_525809.2
Saccharomyces cerevisiae (baker's yeast)	RKI1	NP_014738.1
Canis lupus familiaris (dog)	RPIA	XP_003639654.1
Rattus norvegicus (Norway rat)	Rpia	NP_001102102.1
Caenorhabditis elegans (roundworm)	rpia-1	NP_498556.1
Eremothecium gossypii	AGOS_ACL077C	NP_983327.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.
Heintze J, Costa JR, Weber M, Ketteler R
Cellular signalling28(9)1380-8(2016 Sep)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results are consistent with a model in which RPIA suppresses autophagy and LC3 processing by modulation of redox signaling.
Title: Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.

CRC cells that overexpressed miR124 or with knockdown of RPIA or PRPS1 had reduced DNA synthesis and proliferation, whereas cells incubated with an inhibitor of miR124 had significantly increased DNA synthesis and proliferation and formed more colonies.
Title: MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.

Study provides new insight into the molecular mechanisms by which RPIA overexpression can induce oncogenesis in hepatocellular carcinoma.
Title: Ribose-5-phosphate isomerase A regulates hepatocarcinogenesis via PP2A and ERK signaling.

In this work, through an in silico comparative analysis between the genomes of Leishmania major and Homo sapiens, the enzyme ribose 5-phosphate isomerase (R5PI) was indicated as a promising molecular target.
Title: Structural modeling and docking studies of ribose 5-phosphate isomerase from Leishmania major and Homo sapiens: a comparative analysis for Leishmaniasis treatment.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

The following RPIA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPIA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18642	NM_144563.3 Latest version!	Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu18642	NM_144563.2	Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPIA ( NM_144563.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653164.2 Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.

Price & Availability↑

CloneID	OHu18642
Clone ID Related Accession (Same CDS sequence)	NM_144563.2 , NM_144563.3
Accession Version	NM_144563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	ribose-5-phosphate isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB989690.1, BC015529.1 and BF063086.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2006 this sequence version replaced NM_144563.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY050633.1, SRR1163658.391040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCGCC CCGGGCCCTT CAGCACCCTC TACGGGCGGG TCTTGGCCCC GCTGCCCGGG 
AGGGCCGGGG GCGCGGCCTC CGGCGGAGGA GGGAACAGCT GGGACCTCCC GGGTTCCCAC 
GTGCGGCTGC CGGGGCGTGC ACAGTCTGGG ACCCGTGGCG GTGCTGGCAA CACAAGCACC 
AGCTGCGGGG ACTCCAACAG CATCTGCCCG GCCCCCTCCA CGATGTCCAA GGCCGAGGAG 
GCCAAGAAGC TGGCGGGCCG CGCGGCTGTG GAGAACCACG TGAGGAATAA CCAAGTGCTG 
GGAATTGGAA GTGGTTCTAC AATTGTCCAT GCTGTGCAGC GAATAGCTGA AAGGGTGAAG 
CAAGAGAATC TGAACCTCGT CTGTATTCCC ACTTCCTTCC AGGCCCGCCA GCTCATCCTG 
CAGTATGGCT TGACCCTCAG TGATCTGGAT CGACACCCAG AGATCGACCT TGCCATCGAT 
GGTGCTGATG AAGTAGATGC TGATCTCAAT CTCATCAAGG GTGGCGGAGG CTGCCTGACC 
CAGGAGAAGA TTGTGGCTGG CTATGCTAGT CGCTTCATCG TGATCGCTGA TTTCAGGAAA 
GATTCGAAGA ATCTCGGGGA TCAGTGGCAC AAGGGAATCC CCATCGAGGT CATCCCAATG 
GCCTATGTCC CAGTGAGCCG AGCTGTGAGC CAGAAGTTTG GGGGCGTGGT TGAACTTCGA 
ATGGCTGTCA ACAAGGCTGG TCCTGTGGTG ACAGATAATG GGAATTTTAT CTTGGACTGG 
AAGTTTGACC GGGTACACAA ATGGAGTGAA GTGAATACAG CTATCAAAAT GATCCCAGGT 
GTGGTGGACA CAGGCCTATT CATCAACATG GCTGAGAGAG TCTACTTTGG GATGCAGGAT 
GGCTCAGTGA ACATGAGGGA GAAGCCTTTC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653164.2
CDS	42..977
Translation	MQRPGPFSTLYGRVLAPLPGRAGGAASGGGGNSWDLPGSHVRLPGRAQSGTRGGAGNTSTSCGDSNSICPAPSTMSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREKPFC

Target ORF information:

RefSeq Version	NM_144563.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144563.2

ORF Insert Sequence:

ATGCAGCGCC CCGGGCCCTT CAGCACCCTC TACGGGCGGG TCTTGGCCCC GCTGCCCGGG 
AGGGCCGGGG GCGCGGCCTC CGGCGGAGGA GGGAACAGCT GGGACCTCCC GGGTTCCCAC 
GTGCGGCTGC CGGGGCGTGC ACAGTCTGGG ACCCGTGGCG GTGCTGGCAA CACAAGCACC 
AGCTGCGGGG ACTCCAACAG CATCTGCCCG GCCCCCTCCA CGATGTCCAA GGCCGAGGAG 
GCCAAGAAGC TGGCGGGCCG CGCGGCTGTG GAGAACCACG TGAGGAATAA CCAAGTGCTG 
GGAATTGGAA GTGGTTCTAC AATTGTCCAT GCTGTGCAGC GAATAGCTGA AAGGGTGAAG 
CAAGAGAATC TGAACCTCGT CTGTATTCCC ACTTCCTTCC AGGCCCGCCA GCTCATCCTG 
CAGTATGGCT TGACCCTCAG TGATCTGGAT CGACACCCAG AGATCGACCT TGCCATCGAT 
GGTGCTGATG AAGTAGATGC TGATCTCAAT CTCATCAAGG GTGGCGGAGG CTGCCTGACC 
CAGGAGAAGA TTGTGGCTGG CTATGCTAGT CGCTTCATCG TGATCGCTGA TTTCAGGAAA 
GATTCGAAGA ATCTCGGGGA TCAGTGGCAC AAGGGAATCC CCATCGAGGT CATCCCAATG 
GCCTATGTCC CAGTGAGCCG AGCTGTGAGC CAGAAGTTTG GGGGCGTGGT TGAACTTCGA 
ATGGCTGTCA ACAAGGCTGG TCCTGTGGTG ACAGATAATG GGAATTTTAT CTTGGACTGG 
AAGTTTGACC GGGTACACAA ATGGAGTGAA GTGAATACAG CTATCAAAAT GATCCCAGGT 
GTGGTGGACA CAGGCCTATT CATCAACATG GCTGAGAGAG TCTACTTTGG GATGCAGGAT 
GGCTCAGTGA ACATGAGGGA GAAGCCTTTC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPIA ( NM_144563.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653164.2 Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.

Price & Availability↑

CloneID	OHu18642
Clone ID Related Accession (Same CDS sequence)	NM_144563.2 , NM_144563.3
Accession Version	NM_144563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ribose-5-phosphate isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB989690.1, BC015529.1 and BF063086.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_144563.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.218873.1, SRR3476690.319943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000283646.5/ ENSP00000283646.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCGCC CCGGGCCCTT CAGCACCCTC TACGGGCGGG TCTTGGCCCC GCTGCCCGGG 
AGGGCCGGGG GCGCGGCCTC CGGCGGAGGA GGGAACAGCT GGGACCTCCC GGGTTCCCAC 
GTGCGGCTGC CGGGGCGTGC ACAGTCTGGG ACCCGTGGCG GTGCTGGCAA CACAAGCACC 
AGCTGCGGGG ACTCCAACAG CATCTGCCCG GCCCCCTCCA CGATGTCCAA GGCCGAGGAG 
GCCAAGAAGC TGGCGGGCCG CGCGGCTGTG GAGAACCACG TGAGGAATAA CCAAGTGCTG 
GGAATTGGAA GTGGTTCTAC AATTGTCCAT GCTGTGCAGC GAATAGCTGA AAGGGTGAAG 
CAAGAGAATC TGAACCTCGT CTGTATTCCC ACTTCCTTCC AGGCCCGCCA GCTCATCCTG 
CAGTATGGCT TGACCCTCAG TGATCTGGAT CGACACCCAG AGATCGACCT TGCCATCGAT 
GGTGCTGATG AAGTAGATGC TGATCTCAAT CTCATCAAGG GTGGCGGAGG CTGCCTGACC 
CAGGAGAAGA TTGTGGCTGG CTATGCTAGT CGCTTCATCG TGATCGCTGA TTTCAGGAAA 
GATTCGAAGA ATCTCGGGGA TCAGTGGCAC AAGGGAATCC CCATCGAGGT CATCCCAATG 
GCCTATGTCC CAGTGAGCCG AGCTGTGAGC CAGAAGTTTG GGGGCGTGGT TGAACTTCGA 
ATGGCTGTCA ACAAGGCTGG TCCTGTGGTG ACAGATAATG GGAATTTTAT CTTGGACTGG 
AAGTTTGACC GGGTACACAA ATGGAGTGAA GTGAATACAG CTATCAAAAT GATCCCAGGT 
GTGGTGGACA CAGGCCTATT CATCAACATG GCTGAGAGAG TCTACTTTGG GATGCAGGAT 
GGCTCAGTGA ACATGAGGGA GAAGCCTTTC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653164.2
CDS	27..962
Translation	MQRPGPFSTLYGRVLAPLPGRAGGAASGGGGNSWDLPGSHVRLPGRAQSGTRGGAGNTSTSCGDSNSICPAPSTMSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREKPFC

Target ORF information:

RefSeq Version	NM_144563.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribose 5-phosphate isomerase A (RPIA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144563.3

ORF Insert Sequence:

ATGCAGCGCC CCGGGCCCTT CAGCACCCTC TACGGGCGGG TCTTGGCCCC GCTGCCCGGG 
AGGGCCGGGG GCGCGGCCTC CGGCGGAGGA GGGAACAGCT GGGACCTCCC GGGTTCCCAC 
GTGCGGCTGC CGGGGCGTGC ACAGTCTGGG ACCCGTGGCG GTGCTGGCAA CACAAGCACC 
AGCTGCGGGG ACTCCAACAG CATCTGCCCG GCCCCCTCCA CGATGTCCAA GGCCGAGGAG 
GCCAAGAAGC TGGCGGGCCG CGCGGCTGTG GAGAACCACG TGAGGAATAA CCAAGTGCTG 
GGAATTGGAA GTGGTTCTAC AATTGTCCAT GCTGTGCAGC GAATAGCTGA AAGGGTGAAG 
CAAGAGAATC TGAACCTCGT CTGTATTCCC ACTTCCTTCC AGGCCCGCCA GCTCATCCTG 
CAGTATGGCT TGACCCTCAG TGATCTGGAT CGACACCCAG AGATCGACCT TGCCATCGAT 
GGTGCTGATG AAGTAGATGC TGATCTCAAT CTCATCAAGG GTGGCGGAGG CTGCCTGACC 
CAGGAGAAGA TTGTGGCTGG CTATGCTAGT CGCTTCATCG TGATCGCTGA TTTCAGGAAA 
GATTCGAAGA ATCTCGGGGA TCAGTGGCAC AAGGGAATCC CCATCGAGGT CATCCCAATG 
GCCTATGTCC CAGTGAGCCG AGCTGTGAGC CAGAAGTTTG GGGGCGTGGT TGAACTTCGA 
ATGGCTGTCA ACAAGGCTGG TCCTGTGGTG ACAGATAATG GGAATTTTAT CTTGGACTGG 
AAGTTTGACC GGGTACACAA ATGGAGTGAA GTGAATACAG CTATCAAAAT GATCCCAGGT 
GTGGTGGACA CAGGCCTATT CATCAACATG GCTGAGAGAG TCTACTTTGG GATGCAGGAT 
GGCTCAGTGA ACATGAGGGA GAAGCCTTTC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.
Cellular signalling28(9)1380-8(2016 Sep)
Heintze J,Costa JR,Weber M,Ketteler R

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

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RPIA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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