ALDOB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALDOB
Entrez Gene ID	229
Full Name	aldolase, fructose-bisphosphate B
Synonyms	ALDB,ALDO2
General protein information	Preferred Names aldolase, fructose-bisphosphate B Names fructose-bisphosphate aldolase B aldolase 2 aldolase B, fructose-bisphosphatase aldolase B, fructose-bisphosphate liver-type aldolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q31.1
Summary	Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008].
Disorder MIM:	612724
Disorder Html:	Fructose intolerance, hereditary, 229600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000035.3	NP_000026.2	fructose-bisphosphate aldolase B
NM_000035.4	NP_000026.2	fructose-bisphosphate aldolase B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALDOB	NP_000026.2
Gallus gallus (chicken)	ALDOB	NP_001007978.1
Canis lupus familiaris (dog)	ALDOB	XP_867333.3
Bos taurus (cattle)	ALDOB	NP_001029657.1
Mus musculus (house mouse)	Aldob	NP_659152.1
Rattus norvegicus (Norway rat)	Aldob	NP_036628.2
Danio rerio (zebrafish)	aldob	NP_919348.3
	ALDOB	XP_001111095.1
Pan troglodytes (chimpanzee)	ALDOB	XP_001136159.1
Xenopus tropicalis (tropical clawed frog)	aldob	NP_989131.1

Gene Ontology
Bibliography

Related articles in PubMed

Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma.
Li Q, Li Y, Xu J, Wang S, Xu Y, Li X, Cai S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(1)397-406(2017)

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.
Tao QF, Yuan SX, Yang F, Yang S, Yang Y, Yuan JH, Wang ZG, Xu QG, Lin KY, Cai J, Yu J, Huang WL, Teng XL, Zhou CC, Wang F, Sun SH, Zhou WP
Molecular cancer14170(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing Aldolase B activated epithelial markers and repressed mesenchymal markers, indicating inactivation of Aldolase B may lead to inhibition of epithelial-mesenchymal transition
Title: Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma.

The downregulation of ALDOB could indicate a poor prognosis for HCC patients, and therefore, ALDOB might be considered a prognostic biomarker for HCC, especially at the early stage.
Title: Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling.
Title: Aldolase positively regulates of the canonical Wnt signaling pathway.

Single nucleotide polymorphisms in ALDOB, MAP3K1, and MEF2C are associated with cataract.
Title: Electronic medical records and genomics (eMERGE) network exploration in cataract: several new potential susceptibility loci.

both of exogenous and endogenous ALDOB proteins bind to hepatitis B surface antigen and colocalize in the cytoplasm in vitro and inhibit apoptosis of cisplatin-induced HepG2 cells.
Title: ALDOB acts as a novel HBsAg-binding protein and its coexistence inhibits cisplatin-induced HepG2 cell apoptosis.

The following ALDOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALDOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18947	NM_000035.4 Latest version!	Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu18947	NM_000035.3	Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALDOB ( NM_000035.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000026.2 Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.

Price & Availability↑

CloneID	OHu18947
Clone ID Related Accession (Same CDS sequence)	NM_000035.3 , NM_000035.4
Accession Version	NM_000035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	fructose-bisphosphate aldolase B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02747.1, AL353621.19 and AW242415.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2008 this sequence version replaced NM_000035.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02747.1, X01098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG 
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA 
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA 
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA 
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC 
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG 
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA 
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT 
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT 
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC 
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC 
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA 
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC 
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000026.2
CDS	83..1177
Translation	MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

Target ORF information:

RefSeq Version	NM_000035.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000035.3

ORF Insert Sequence:

ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG 
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA 
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA 
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA 
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC 
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG 
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA 
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT 
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT 
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC 
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC 
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA 
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC 
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALDOB ( NM_000035.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000026.2 Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.

Price & Availability↑

CloneID	OHu18947
Clone ID Related Accession (Same CDS sequence)	NM_000035.3 , NM_000035.4
Accession Version	NM_000035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	fructose-bisphosphate aldolase B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02747.1, AL353621.19 and AW242415.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2018 this sequence version replaced NM_000035.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290795.1, SRR5189664.143737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000647789.2/ ENSP00000497767.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG 
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA 
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA 
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA 
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC 
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG 
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA 
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT 
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT 
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC 
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC 
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA 
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC 
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000026.2
CDS	77..1171
Translation	MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

Target ORF information:

RefSeq Version	NM_000035.4
Organism	Homo sapiens(human)
Definition	Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000035.4

ORF Insert Sequence:

ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG 
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA 
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC 
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA 
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA 
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC 
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG 
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA 
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT 
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT 
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC 
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC 
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC 
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA 
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC 
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(1)397-406(2017)
Li Q,Li Y,Xu J,Wang S,Xu Y,Li X,Cai S

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.
Molecular cancer14170(2015 Sep)
Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP

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ALDOB cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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